Hydrogels with multifunctionalities,including sufficient bonding strength,injectability and self-healing capacity,responsive-adhesive ability,fault-tolerant and repeated tissue adhesion,are urgently demanded for invas...Hydrogels with multifunctionalities,including sufficient bonding strength,injectability and self-healing capacity,responsive-adhesive ability,fault-tolerant and repeated tissue adhesion,are urgently demanded for invasive wound closure and wound healing.Motivated by the adhesive mechanism of mussel and brown algae,bioinspired dynamic bonds cross-linked multifunctional hydrogel adhesive is designed based on sodium alginate(SA),gelatin(GT)and protocatechualdehyde,with ferric ions added,for sutureless post-wound-closure.The dynamic hydrogel cross-linked through Schiff base bond,catechol-Fe coordinate bond and the strong interaction between GT with temperature-dependent phase transition and SA,endows the resulting hydrogel with sufficient mechanical and adhesive strength for efficient wound closure,injectability and self-healing capacity,and repeated closure of reopened wounds.Moreover,the temperature-dependent adhesive properties endowed mispositioning hydrogel to be removed/repositioned,which is conducive for the fault-tolerant adhesion of the hydrogel adhesives during surgery.Besides,the hydrogels present good biocompatibility,near-infrared-assisted photothermal antibacterial activity,antioxidation and repeated thermo-responsive reversible adhesion and good hemostatic effect.The in vivo incision closure evaluation demonstrated their capability to promote the post-wound-closure and wound healing of the incisions,indicating that the developed reversible adhesive hydrogel dressing could serve as versatile tissue sealant.展开更多
Due to the lack of an ideal material for TMJ(temporomandibular joint)disc perforation and local inflammation interfering with tissue regeneration,a functional TGI/HA-CS(tilapia type I gelatin/hyaluronic acid-chondroit...Due to the lack of an ideal material for TMJ(temporomandibular joint)disc perforation and local inflammation interfering with tissue regeneration,a functional TGI/HA-CS(tilapia type I gelatin/hyaluronic acid-chondroitin sulfate)double network hydrogel was constructed in this paper.It was not only multiply bionic in its composition,structure and mechanical strength,but also endowed with the ability to immunomodulate microenvironment and simultaneously induce in situ repair of defected TMJ discs.On the one hand,it inhibited inflammatory effects of inflammasome in macrophages,reduced the extracellular matrix(ECM)-degrading enzymes secreted by chondrocytes,reversed the local inflammatory state,promoted the proliferation of TMJ disc cells and induced fibrochondrogenic differentiation of synovium-derived mesenchymal stem cells(SMSCs).On the other hand,it gave an impetus to repairing a relatively-large(6 mm-sized)defect in mini pigs’TMJ discs in a rapid and high-quality manner,which suggested a promising clinical application.展开更多
基金supported by the National Natural Science Foundation of China (No. 51973172)Natural Science Foundation of Shaanxi Province (Nos. 2020JC-03 and 2019TD-020)+2 种基金the State Key Laboratory for Mechanical Behavior of Materials,the World-Class Universities (Disciplines) and Characteristic Development Guidance Funds for the Central UniversitiesFundamental Research Funds for the Central Universitiesthe Opening Project of the Key Laboratory of Shaanxi Province for Craniofacial Precision Medicine Research,College of Stomatology,Xi’an Jiaotong University (No. 2019LHM-KFKT008).
文摘Hydrogels with multifunctionalities,including sufficient bonding strength,injectability and self-healing capacity,responsive-adhesive ability,fault-tolerant and repeated tissue adhesion,are urgently demanded for invasive wound closure and wound healing.Motivated by the adhesive mechanism of mussel and brown algae,bioinspired dynamic bonds cross-linked multifunctional hydrogel adhesive is designed based on sodium alginate(SA),gelatin(GT)and protocatechualdehyde,with ferric ions added,for sutureless post-wound-closure.The dynamic hydrogel cross-linked through Schiff base bond,catechol-Fe coordinate bond and the strong interaction between GT with temperature-dependent phase transition and SA,endows the resulting hydrogel with sufficient mechanical and adhesive strength for efficient wound closure,injectability and self-healing capacity,and repeated closure of reopened wounds.Moreover,the temperature-dependent adhesive properties endowed mispositioning hydrogel to be removed/repositioned,which is conducive for the fault-tolerant adhesion of the hydrogel adhesives during surgery.Besides,the hydrogels present good biocompatibility,near-infrared-assisted photothermal antibacterial activity,antioxidation and repeated thermo-responsive reversible adhesion and good hemostatic effect.The in vivo incision closure evaluation demonstrated their capability to promote the post-wound-closure and wound healing of the incisions,indicating that the developed reversible adhesive hydrogel dressing could serve as versatile tissue sealant.
基金This work was sponsored by grants from the National Nature Science Foundation of China(82072070)the National Key Research and Development Program of China(2018YFC1105202)Science and Technology Commission of Shanghai Municipality(21DZ2291700,18DZ2290300).
文摘Due to the lack of an ideal material for TMJ(temporomandibular joint)disc perforation and local inflammation interfering with tissue regeneration,a functional TGI/HA-CS(tilapia type I gelatin/hyaluronic acid-chondroitin sulfate)double network hydrogel was constructed in this paper.It was not only multiply bionic in its composition,structure and mechanical strength,but also endowed with the ability to immunomodulate microenvironment and simultaneously induce in situ repair of defected TMJ discs.On the one hand,it inhibited inflammatory effects of inflammasome in macrophages,reduced the extracellular matrix(ECM)-degrading enzymes secreted by chondrocytes,reversed the local inflammatory state,promoted the proliferation of TMJ disc cells and induced fibrochondrogenic differentiation of synovium-derived mesenchymal stem cells(SMSCs).On the other hand,it gave an impetus to repairing a relatively-large(6 mm-sized)defect in mini pigs’TMJ discs in a rapid and high-quality manner,which suggested a promising clinical application.
