A novel thermophilic two-ring bioreactor was employed to produce biohythane from palm oil mill effluent(POME)using Thermoanaerobacterium thermosaccharolyticum PSU-2 and thermophilic methanogenic mixed cul-tures.The re...A novel thermophilic two-ring bioreactor was employed to produce biohythane from palm oil mill effluent(POME)using Thermoanaerobacterium thermosaccharolyticum PSU-2 and thermophilic methanogenic mixed cul-tures.The reactor system demonstrated impressive performance,achieving hydrogen and methane yields of 113.3±15.0 mL/g-VS and 473.0±60.0 mL/g-VS,respectively,with a total biohythane composition of 4.3%H_(2),37.1%CO_(2),and 58.6%CH_(4).The process exhibited high treatment efficiency,with COD and VS removal efficiencies reaching 93.7% and 84.3%,respectively.Microbial community analysis revealed the crucial roles of various microorganisms in the biohythane production process.Thermoclostridium sp.,Thermoanaerobacterium sp.,and Anaerobranca sp.were identified as key players in hydrogen production,while Bacteroides sp.and Meth-anobacterium sp.were found to be essential for methane production.The optimization of operating conditions,including pH(5.0-8.0),temperature(55℃),and hydraulic retention time(2 d for H_(2) and 10 d for CH_(4)),significantly enhanced biohythane production efficiency.The techno-economic analysis demonstrated the eco-nomic viability of the thermophilic two-ring bioreactor system,with a net present value of 4,186,000 USD,an internal rate of return of 82%,and a payback period of 1.4 years.These findings highlight the potential of this innovative technology as a sustainable and economically attractive solution for treating POME and simulta-neously producing renewable energy in the form of biohythane,contributing to the sustainable development of the palm oil industry and the mitigation of greenhouse gas emissions.展开更多
基金the Royal Golden Jubilee Ph.D.Grant[2.B.TK/60/A.1]the National Research Council of Thailand(NRCT)through the Talented Mid-Career Research Grant(Grant No.N41A640088the Graduate Education Development Fund for the Fiscal Year 2021 for their generous financial support,which made this research possible.
文摘A novel thermophilic two-ring bioreactor was employed to produce biohythane from palm oil mill effluent(POME)using Thermoanaerobacterium thermosaccharolyticum PSU-2 and thermophilic methanogenic mixed cul-tures.The reactor system demonstrated impressive performance,achieving hydrogen and methane yields of 113.3±15.0 mL/g-VS and 473.0±60.0 mL/g-VS,respectively,with a total biohythane composition of 4.3%H_(2),37.1%CO_(2),and 58.6%CH_(4).The process exhibited high treatment efficiency,with COD and VS removal efficiencies reaching 93.7% and 84.3%,respectively.Microbial community analysis revealed the crucial roles of various microorganisms in the biohythane production process.Thermoclostridium sp.,Thermoanaerobacterium sp.,and Anaerobranca sp.were identified as key players in hydrogen production,while Bacteroides sp.and Meth-anobacterium sp.were found to be essential for methane production.The optimization of operating conditions,including pH(5.0-8.0),temperature(55℃),and hydraulic retention time(2 d for H_(2) and 10 d for CH_(4)),significantly enhanced biohythane production efficiency.The techno-economic analysis demonstrated the eco-nomic viability of the thermophilic two-ring bioreactor system,with a net present value of 4,186,000 USD,an internal rate of return of 82%,and a payback period of 1.4 years.These findings highlight the potential of this innovative technology as a sustainable and economically attractive solution for treating POME and simulta-neously producing renewable energy in the form of biohythane,contributing to the sustainable development of the palm oil industry and the mitigation of greenhouse gas emissions.
