To improve the nutritional and functional value of rice,numerous biotechnological approaches have focused on metabolic engineering to address nutritional deficiencies and produce health-beneficial compounds that are e...To improve the nutritional and functional value of rice,numerous biotechnological approaches have focused on metabolic engineering to address nutritional deficiencies and produce health-beneficial compounds that are either absent or naturally present in low amounts.A prominent example is‘Golden Rice’,which has been genetically modified to accumulateβ-carotene to combat vitamin A deficiency in regions with limited dietary intake.Scientists have been continuously biofortifying rice with various specialized metabolites,including terpenoids,flavonoids,non-flavonoid polyphenols,betalains,vitamins,and amino acids.This review explores the specific pathways and genetic modifications utilized by researchers to enhance the accumulation of targeted metabolites in rice.It comprehensively summarizes key strategies and research trends in rice metabolic engineering,demonstrating how rice can be transformed into a strategic crop for producing industrially valuable compounds beyond its traditional role as a staple food by leveraging its advantages as a versatile host system through its grains,leaves,and cells.Furthermore,we highlight the potential of intergrating metabolic engineering with synthetic biology and big data-driven computational modeling,particularly through artificial intelligence and machine learning,as promising future research directions.展开更多
Synthetic biology provides a new paradigm for life science research(“build to learn”)and opens the future journey of biotechnology(“build to use”).Here,we discuss advances of various principles and technologies in...Synthetic biology provides a new paradigm for life science research(“build to learn”)and opens the future journey of biotechnology(“build to use”).Here,we discuss advances of various principles and technologies in the mainstream of the enabling technology of synthetic biology,including synthesis and assembly of a genome,DNA storage,gene editing,molecular evolution and de novo design of function proteins,cell and gene circuit engineering,cell-free synthetic biology,artificial intelligence(AI)-aided synthetic biology,as well as biofoundries.We also introduce the concept of quantitative synthetic biology,which is guiding synthetic biology towards increased accuracy and predictability or the real rational design.We conclude that synthetic biology will establish its disciplinary system with the iterative development of enabling technologies and the maturity of the core theory.展开更多
The past decade has been envisaged as a period of unprecedented growth and development in the bioprocessing industry due to the increasing prominence of manufacturing bioproducts encompassing day-to-day life.Bioproces...The past decade has been envisaged as a period of unprecedented growth and development in the bioprocessing industry due to the increasing prominence of manufacturing bioproducts encompassing day-to-day life.Bioprocesses are the heart of biotechnology and represent the most dynamic constituent for conceptualizing the bioeconomy as it has the potential to tackle the most burgeoning problems such as climatic adversity,global population growth,reduced ecosystem resilience.The promising amalgamation of digitalization,biologicalization,and biomanufacturing paved the way for an emerging concept of“bio-intelligent value addition”or more prominently Bioprocessing 4.0 that enables the transformation in the landscape of biomanufacturing.Despite its positive credentials,the technology is facing technical,organizational,economical,and likely some unforeseen challenges that must be resolved for its successful implementation for hailing the sustainability development goals(SDGs)of bioeconomy.Though the road of bioeconomy is quite arduous,the continuous demand for bioproducts and their timely delivery at a faster rate necessitates the culture of sharing knowledge,digitalization,automa-tion,and development of flexible modular and podular facility footprints to accelerate biomanufacturing.Therefore,it is worth summarizing the major portfolios of Bioprocessing 4.0 such as conception of biofoundry,bioprocess intensification strategies,process and data analytics,software and automation,and its synergistic correlation with bioeconomy.Thus,the present article advocates about the technological glance of Bioprocessing 4.0 along with technical challenges and future research priorities for sparking the glory of this industrial landscape for enshrining the bioeconomy.展开更多
基金supported by the Bio&Medical Technology Development Program of the National Research Foundation(NRF)funded by the Korean government(MSIT)(Grant No.RS-2024-00440478)to Sun-Hwa HAthe NRF by MSIT(Grant Nos.RS-2024-00347806 and RS-2024-00407469)to Sun-Hwa HAthe New Plant Breed Technology Program funded by the Rural Development Administration,Republic of Korea(Grant No.RS-2024-00322447)to Sun-Hwa HA.
文摘To improve the nutritional and functional value of rice,numerous biotechnological approaches have focused on metabolic engineering to address nutritional deficiencies and produce health-beneficial compounds that are either absent or naturally present in low amounts.A prominent example is‘Golden Rice’,which has been genetically modified to accumulateβ-carotene to combat vitamin A deficiency in regions with limited dietary intake.Scientists have been continuously biofortifying rice with various specialized metabolites,including terpenoids,flavonoids,non-flavonoid polyphenols,betalains,vitamins,and amino acids.This review explores the specific pathways and genetic modifications utilized by researchers to enhance the accumulation of targeted metabolites in rice.It comprehensively summarizes key strategies and research trends in rice metabolic engineering,demonstrating how rice can be transformed into a strategic crop for producing industrially valuable compounds beyond its traditional role as a staple food by leveraging its advantages as a versatile host system through its grains,leaves,and cells.Furthermore,we highlight the potential of intergrating metabolic engineering with synthetic biology and big data-driven computational modeling,particularly through artificial intelligence and machine learning,as promising future research directions.
基金supported by the Strategic Priority Research Program of the Chinese Academy of Sciences(XDB29050100,XDB29050500,XDA24020102)to X.E.Zhang,C.Liu and C.Gao,respectivelythe National Natural Science Foundation of China(31725002,31861143017,32022044,62050152 and 32071428)to J.Dai,Y.Yuan,C.You,and X.Wang,respectivelythe National Key Research and Development Program of China(2020YFA0907700,2018YFA0901600,2019YFA09004500)to Y.Feng and P.Wei。
文摘Synthetic biology provides a new paradigm for life science research(“build to learn”)and opens the future journey of biotechnology(“build to use”).Here,we discuss advances of various principles and technologies in the mainstream of the enabling technology of synthetic biology,including synthesis and assembly of a genome,DNA storage,gene editing,molecular evolution and de novo design of function proteins,cell and gene circuit engineering,cell-free synthetic biology,artificial intelligence(AI)-aided synthetic biology,as well as biofoundries.We also introduce the concept of quantitative synthetic biology,which is guiding synthetic biology towards increased accuracy and predictability or the real rational design.We conclude that synthetic biology will establish its disciplinary system with the iterative development of enabling technologies and the maturity of the core theory.
文摘The past decade has been envisaged as a period of unprecedented growth and development in the bioprocessing industry due to the increasing prominence of manufacturing bioproducts encompassing day-to-day life.Bioprocesses are the heart of biotechnology and represent the most dynamic constituent for conceptualizing the bioeconomy as it has the potential to tackle the most burgeoning problems such as climatic adversity,global population growth,reduced ecosystem resilience.The promising amalgamation of digitalization,biologicalization,and biomanufacturing paved the way for an emerging concept of“bio-intelligent value addition”or more prominently Bioprocessing 4.0 that enables the transformation in the landscape of biomanufacturing.Despite its positive credentials,the technology is facing technical,organizational,economical,and likely some unforeseen challenges that must be resolved for its successful implementation for hailing the sustainability development goals(SDGs)of bioeconomy.Though the road of bioeconomy is quite arduous,the continuous demand for bioproducts and their timely delivery at a faster rate necessitates the culture of sharing knowledge,digitalization,automa-tion,and development of flexible modular and podular facility footprints to accelerate biomanufacturing.Therefore,it is worth summarizing the major portfolios of Bioprocessing 4.0 such as conception of biofoundry,bioprocess intensification strategies,process and data analytics,software and automation,and its synergistic correlation with bioeconomy.Thus,the present article advocates about the technological glance of Bioprocessing 4.0 along with technical challenges and future research priorities for sparking the glory of this industrial landscape for enshrining the bioeconomy.
