This study introduces a nanostructured MgO coating fabricated via anodization in a non-aqueous electrolyte,offering a novel approach to addressing the challenges of corrosion resistance and biofunctionality.The surfac...This study introduces a nanostructured MgO coating fabricated via anodization in a non-aqueous electrolyte,offering a novel approach to addressing the challenges of corrosion resistance and biofunctionality.The surface was characterized before and after immersion testing using field emission scanning electron microscopy(FESEM),energy-dispersive X-ray spectroscopy(EDX),and X-ray diffraction(XRD).Electrochemical impedance spectroscopy(EIS)and potentiodynamic polarization tests demonstrated a 2-fold reduction in the corrosion resistance compared to untreated magnesium.Biomineralization studies demonstrated the uniform formation of apatite with a Ca/P ratio of 1.35 on the nanostructured surface after 14 days in simulated body fluid(SBF),surpassing that of microstructured MgO.Hydrogen evolution decreased from 912±38μL cm^(-2)for untreated Mg to 615±32μL cm^(-2)for the Mg/MgO nanostructure and 545±29μL cm^(-2)for the Mg/Mg O/HA sample.These findings highlight the potential of nanostructured MgO coatings to advance Mg-based implants by providing enhanced corrosion protection,improved biomineralization,reduced hemolysis and increased cell viability,and reduced H_(2)generation.展开更多
Surface modification of medical implants was considered as an effective method to improve the cellular behaviors and the integration of tissue onto materials. The micro-nanostructured surface on the titanium alloy was...Surface modification of medical implants was considered as an effective method to improve the cellular behaviors and the integration of tissue onto materials. The micro-nanostructured surface on the titanium alloy was prepared by laser treatment and multiple acid etching. The surface morphologies of different titanium alloy substrates were characterized by scanning electron microscopy (SEM). The effects of micro-nanostructured surfaces on the cellular responses were investigated in vitro by observing hydroxyapatite formation, cell morphology and cell adhesion. The results indicate that the micro-sized structure promoted the adhesion and proliferation of cultured osteoblasts. Furthermore, the micro-nanostructured surface was more conducive to cell adhension stretching compared with the micro-structured surface. All results suggest that the micro-nanostructured surface improved the biocompatibility and integration of tissue onto titanium alloy implants.展开更多
In order to develop a new plant-source insecticide,some active components from Cichorium intybus L.were extracted with mineral ether,ethyl ether,ethyl acetate,respectively.It were testified the effects of the componen...In order to develop a new plant-source insecticide,some active components from Cichorium intybus L.were extracted with mineral ether,ethyl ether,ethyl acetate,respectively.It were testified the effects of the components on the development of Mythimna separate Walker and anti-feeding by feeding and weighting method.The results showed that the body weight of the larvae fed by the extracts with organic solvents was significantly lower than the control;the body weight of the larvae fed by the extracts with organic solvents was significantly different in different solvents in 3-5 days.The corrected mortality of the ethyl acetate treatment was the highest（52.05%）,and the development period of the larvae treated by ethyl acetate was about 4-10 days longer than the control and the corresponding pupating rate was the lowest（43.30%）.Therefore,the effect of ethyl acetate extract was most significant.Moreover,the pupa weight of the treatments was little more than the control.The extracts from Cichorium intybus L.leaves had highest bioactivity mainly in anti-feeding activity on Mythimna separate Walker.展开更多
背景:随着对生物活性材料的深入研究,各类生物活性材料已成为抗骨感染的重要研究手段与治疗方法。目的:通过对生物活性材料治疗骨感染的相关文献进行可视化分析,探明该领域的研究现状。方法:以中国知网与Web of Science核心合集数据库...背景:随着对生物活性材料的深入研究,各类生物活性材料已成为抗骨感染的重要研究手段与治疗方法。目的:通过对生物活性材料治疗骨感染的相关文献进行可视化分析,探明该领域的研究现状。方法:以中国知网与Web of Science核心合集数据库为检索平台,检索生物活性材料治疗骨感染的相关文献,筛选后导入CiteSpace软件,对发文量、研究机构、作者、文献共被引、关键词等进行可视化分析。结果与结论:(1)根据文献检索及筛选得出中国知网共计纳入相关文献149篇,Web of Science核心合集数据库纳入相关文献1031篇,其中中国发文量最大,美国发文量中心性最高。(2)Web of Science核心合集数据库中发文前3的机构为上海交通大学、四川大学、中国科学院,中国知网发文前3的机构为第三军医大学、昆明医科大学、南方医科大学,其中SHUAI CJ及BOCCACCINI AR两位教授发文最多,HENCH LL教授被引用最高,《Biomaterials》杂志被引用最多。(3)Web of Science核心合集数据库中关键词出现频率最高的前3位为scaffolds、in vitro、bone,中国知网中关键词出现频率最高的前3位为骨髓炎、骨缺损、骨感染,结合关键词及文献共被引分析发现,该领域的研究热点为生物材料与抗菌性能、复合材料与多功能设计以及组织工程与骨再生3个方面。展开更多
背景:人羊膜作为一种天然生物材料,因优异的生物相容性、低免疫原性和丰富的生物活性物质,在组织工程学中展现出广泛应用前景。近年来,针对人羊膜的储存、制备及在组织修复和再生医学中的研究不断深入,推动了多领域的转化与临床应用。目...背景:人羊膜作为一种天然生物材料,因优异的生物相容性、低免疫原性和丰富的生物活性物质,在组织工程学中展现出广泛应用前景。近年来,针对人羊膜的储存、制备及在组织修复和再生医学中的研究不断深入,推动了多领域的转化与临床应用。目的:综述人羊膜的储存、制备、改性及在组织工程和再生医学中的研究进展,为人羊膜的进一步开发与临床应用提供理论依据。方法:通过计算机检索中国知网、中国生物医学文献数据库、万方数据库、PubMed、Web of Science、Scopus以及Elsevier数据库,中文检索词为“人羊膜,人羊膜的储存,人羊膜的改性,人羊膜的应用,组织工程学”。英文检索词为“Human amniotic membrane,modification of human amniotic membrane,Amniotic membrane modification,Tissue engineering,Stem cells,Bioactive factors,Scaffold,Regenerative medicine”。对初步检索到的文章标题和摘要进行阅读,排除不符合要求的文章,随后根据纳入及排除标准对文章全文进行阅读,最终纳入72篇符合要求的文章。结果与结论:人羊膜具有出色的生物相容性且含有多种活性因子,通过对人羊膜储存和改性技术的不断优化,其应用形式得到进一步拓展,如支架材料、补片等应用形式,同时其力学性能、降解速率和细胞黏附能力都得到了有效提升,可为软组织修复和再生提供支持,未来应关注人羊膜与多功能材料的复合应用,深入研究人羊膜作为组织支架、补片及活性因子载体等的作用机制,进一步提升人羊膜在再生医学中的应用价值。展开更多
Salinization of agricultural land is becoming increasingly severe worldwide,posing a significant threat to food security.The exogenous application of bioactive substances has been widely used to enhance plant resistan...Salinization of agricultural land is becoming increasingly severe worldwide,posing a significant threat to food security.The exogenous application of bioactive substances has been widely used to enhance plant resistance to salt stress.In this study,we used corn steep liquor(CSL),myo-inositol(MI),and their combination to improve salt tolerance in Chinese cabbage(Brassica rapa L.ssp.pekinensis)under salt stress conditions.All three treatments significantly increased plant biomass and nutrient uptake,and improved soil physicochemical properties,while alleviating oxidative damage and ion toxicity.展开更多
Preparation of Fe^2＋ chelate of fish protein hydrolysate （Fe-FPH） obtained from low value fish proteins was introduced and its bioactivity was studied by compound enzymolysis. The optimum conditions for hydrolysate...Preparation of Fe^2＋ chelate of fish protein hydrolysate （Fe-FPH） obtained from low value fish proteins was introduced and its bioactivity was studied by compound enzymolysis. The optimum conditions for hydrolysate chelating Fe^2＋ are DH （degree of hydrolysis） at 5%, pH 7.0, 20℃ and 15 min chelating time for FM （material not being defatted）. Four types of Fe-FPH including CA （deposit after chelating）, CB （deposit in 50% of absolute ethanol solution）, CC （suspended deposit in 80% of absolute ethanol solution）, and CD （bottom deposit in 80% of absolute ethanol solution） were fractionated with absolute ethanol from FM. Structural analysis through infra-red spectrum revealed that Fe^2＋ was combined strongly with amino-group and carboxyl-group in each chelate and each Fe^2＋ could form two five-member ring structures. All of the four chelates were shown more significant antioxidative activity and can be used as natural hydrophobic and hydrophilic antioxidant. Among all the chelates, the CB possesses the most effective antioxidative activity at 92% as high as that of a-tocopherol. Among all Fe-FPHs, only CD showed the most effective antibacterial activity against Escherichia coli, Staphylococcus aureus, Salmonella typhi, and Bacillus subtilis and can be used as natural antibacterial. It provides a more effective way for utilization of low value fish proteins and key information of Fe-FPH as additive in food industry.展开更多
Mg-based alloys received significant attention for temporary implant applications while, their applications have been limited by high degradation rate. Therefore, silver-zeolite doped hydroxyapatite（Ag-Zeo-HAp） coat...Mg-based alloys received significant attention for temporary implant applications while, their applications have been limited by high degradation rate. Therefore, silver-zeolite doped hydroxyapatite（Ag-Zeo-HAp） coating was synthesized on Ti O2-coated Mg alloy by physical vapour deposition（PVD） assisted electrodeposition technique to decrease the degradation rate of Mg alloy. X-ray diffraction（XRD） analysis and field emission scanning electron microscopy（FE-SEM） images showed the formation of a uniform and compact layer of Ag-Zeo-HAp with a thickness of 15 μm on the Ti O2 film with a thickness of 1 μm. The potentiodynamic polarization（PDP） and electrochemical impedance spectroscopy（EIS） tests indicated that corrosion resistance of Mg-Ca alloy was considerably increased by the Ag-Zeo-HAp coating. The bioactivity test in the simulated body fluid（SBF） solution showed that a dense and homogeneous bonelike apatite layer was formed on the Ag-Zeo-HAp surface after 14 d. Investigation of antibacterial activity via disk diffusion and spread plate methods showed that the Ag-Zeo-HAp coating had a significantly larger inhibition zone（3.86 mm） towards Escherichia coli（E. coli） compared with the Ti O2-coated Mg alloy（2.61 mm）. The Ag-Zeo-HAp coating showed high antibacterial performance, good bioactivity, and high corrosion resistance which make it a perfect coating material for biomedical applications.展开更多
The work aims to characterize the structure and to evaluate in vitro the effect of different surface treatments on the bioactivity of medical grade Ti6Al7 Nb alloy implants manufactured by selective laser melting. In ...The work aims to characterize the structure and to evaluate in vitro the effect of different surface treatments on the bioactivity of medical grade Ti6Al7 Nb alloy implants manufactured by selective laser melting. In order to improve the bioactivity of these samples, they were subjected to heat treatment,chemical treatment, and impregnation with bioactive materials. To evaluate the apatite forming ability, the samples were immersed in simulated body fluid solution) and characterized before and after immersion by scanning electron microscopy, X-ray diffraction and X-ray photoelectron spectroscopy.The composition and the texture of the surfaces after the applied treatments have a selective effect on apatite layer development on the surface of samples.展开更多
基金The authors thank the DFG(KI 2169/2-1)the European Union(EU-RIA NOMAD,101091669)for funding this work+1 种基金The Micro and Nanoanalytics Facility(MNaF),funded by the DFG(DFG INST 221/131-1)at the University of Siegen,and the Materials Science Faculty of the Isfahan University of Technology(IUT)were utilized for some of the work and analysis,respectively.
文摘This study introduces a nanostructured MgO coating fabricated via anodization in a non-aqueous electrolyte,offering a novel approach to addressing the challenges of corrosion resistance and biofunctionality.The surface was characterized before and after immersion testing using field emission scanning electron microscopy(FESEM),energy-dispersive X-ray spectroscopy(EDX),and X-ray diffraction(XRD).Electrochemical impedance spectroscopy(EIS)and potentiodynamic polarization tests demonstrated a 2-fold reduction in the corrosion resistance compared to untreated magnesium.Biomineralization studies demonstrated the uniform formation of apatite with a Ca/P ratio of 1.35 on the nanostructured surface after 14 days in simulated body fluid(SBF),surpassing that of microstructured MgO.Hydrogen evolution decreased from 912±38μL cm^(-2)for untreated Mg to 615±32μL cm^(-2)for the Mg/MgO nanostructure and 545±29μL cm^(-2)for the Mg/Mg O/HA sample.These findings highlight the potential of nanostructured MgO coatings to advance Mg-based implants by providing enhanced corrosion protection,improved biomineralization,reduced hemolysis and increased cell viability,and reduced H_(2)generation.
基金Projects(5117530651575320)supported by the National Natural Science Foundation of China+1 种基金Project(TS20130922)supported by the Taishan Scholar Foundation,ChinaProject(2014JC020)supported by the Fundamental Research Funds for the Central Universities of China
文摘Surface modification of medical implants was considered as an effective method to improve the cellular behaviors and the integration of tissue onto materials. The micro-nanostructured surface on the titanium alloy was prepared by laser treatment and multiple acid etching. The surface morphologies of different titanium alloy substrates were characterized by scanning electron microscopy (SEM). The effects of micro-nanostructured surfaces on the cellular responses were investigated in vitro by observing hydroxyapatite formation, cell morphology and cell adhesion. The results indicate that the micro-sized structure promoted the adhesion and proliferation of cultured osteoblasts. Furthermore, the micro-nanostructured surface was more conducive to cell adhension stretching compared with the micro-structured surface. All results suggest that the micro-nanostructured surface improved the biocompatibility and integration of tissue onto titanium alloy implants.
基金Supported by International Cooperation Project from Ministry of Science and Technology"Cooperation Research of Chicory Natural Production Extraction and Identification"(2008DFA31650)Shaanxi International Cooperation Project"Cooperation Research of Chicory Natural Production Extraction and Identification"(2008KW-29)~~
文摘In order to develop a new plant-source insecticide,some active components from Cichorium intybus L.were extracted with mineral ether,ethyl ether,ethyl acetate,respectively.It were testified the effects of the components on the development of Mythimna separate Walker and anti-feeding by feeding and weighting method.The results showed that the body weight of the larvae fed by the extracts with organic solvents was significantly lower than the control;the body weight of the larvae fed by the extracts with organic solvents was significantly different in different solvents in 3-5 days.The corrected mortality of the ethyl acetate treatment was the highest（52.05%）,and the development period of the larvae treated by ethyl acetate was about 4-10 days longer than the control and the corresponding pupating rate was the lowest（43.30%）.Therefore,the effect of ethyl acetate extract was most significant.Moreover,the pupa weight of the treatments was little more than the control.The extracts from Cichorium intybus L.leaves had highest bioactivity mainly in anti-feeding activity on Mythimna separate Walker.
文摘背景:随着对生物活性材料的深入研究,各类生物活性材料已成为抗骨感染的重要研究手段与治疗方法。目的:通过对生物活性材料治疗骨感染的相关文献进行可视化分析,探明该领域的研究现状。方法:以中国知网与Web of Science核心合集数据库为检索平台,检索生物活性材料治疗骨感染的相关文献,筛选后导入CiteSpace软件,对发文量、研究机构、作者、文献共被引、关键词等进行可视化分析。结果与结论:(1)根据文献检索及筛选得出中国知网共计纳入相关文献149篇,Web of Science核心合集数据库纳入相关文献1031篇,其中中国发文量最大,美国发文量中心性最高。(2)Web of Science核心合集数据库中发文前3的机构为上海交通大学、四川大学、中国科学院,中国知网发文前3的机构为第三军医大学、昆明医科大学、南方医科大学,其中SHUAI CJ及BOCCACCINI AR两位教授发文最多,HENCH LL教授被引用最高,《Biomaterials》杂志被引用最多。(3)Web of Science核心合集数据库中关键词出现频率最高的前3位为scaffolds、in vitro、bone,中国知网中关键词出现频率最高的前3位为骨髓炎、骨缺损、骨感染,结合关键词及文献共被引分析发现,该领域的研究热点为生物材料与抗菌性能、复合材料与多功能设计以及组织工程与骨再生3个方面。
文摘背景:人羊膜作为一种天然生物材料,因优异的生物相容性、低免疫原性和丰富的生物活性物质,在组织工程学中展现出广泛应用前景。近年来,针对人羊膜的储存、制备及在组织修复和再生医学中的研究不断深入,推动了多领域的转化与临床应用。目的:综述人羊膜的储存、制备、改性及在组织工程和再生医学中的研究进展,为人羊膜的进一步开发与临床应用提供理论依据。方法:通过计算机检索中国知网、中国生物医学文献数据库、万方数据库、PubMed、Web of Science、Scopus以及Elsevier数据库,中文检索词为“人羊膜,人羊膜的储存,人羊膜的改性,人羊膜的应用,组织工程学”。英文检索词为“Human amniotic membrane,modification of human amniotic membrane,Amniotic membrane modification,Tissue engineering,Stem cells,Bioactive factors,Scaffold,Regenerative medicine”。对初步检索到的文章标题和摘要进行阅读,排除不符合要求的文章,随后根据纳入及排除标准对文章全文进行阅读,最终纳入72篇符合要求的文章。结果与结论:人羊膜具有出色的生物相容性且含有多种活性因子,通过对人羊膜储存和改性技术的不断优化,其应用形式得到进一步拓展,如支架材料、补片等应用形式,同时其力学性能、降解速率和细胞黏附能力都得到了有效提升,可为软组织修复和再生提供支持,未来应关注人羊膜与多功能材料的复合应用,深入研究人羊膜作为组织支架、补片及活性因子载体等的作用机制,进一步提升人羊膜在再生医学中的应用价值。
基金supported by the sub-project“Research and Application of In-Situ Value-Added Water-Soluble Fertilizer Application Technology”(Grant No.2023YFD1700204-3)under the 14th Five-Year National Key R&D Program Project“Development and Industrialization of Novel Green Value-Added Fertilizers”.
文摘Salinization of agricultural land is becoming increasingly severe worldwide,posing a significant threat to food security.The exogenous application of bioactive substances has been widely used to enhance plant resistance to salt stress.In this study,we used corn steep liquor(CSL),myo-inositol(MI),and their combination to improve salt tolerance in Chinese cabbage(Brassica rapa L.ssp.pekinensis)under salt stress conditions.All three treatments significantly increased plant biomass and nutrient uptake,and improved soil physicochemical properties,while alleviating oxidative damage and ion toxicity.
基金Supported by National Natural Science Foundation of China (No.30371123)Science and Technology Department of Zhejiang Province (No. 2007C12013)
文摘Preparation of Fe^2＋ chelate of fish protein hydrolysate （Fe-FPH） obtained from low value fish proteins was introduced and its bioactivity was studied by compound enzymolysis. The optimum conditions for hydrolysate chelating Fe^2＋ are DH （degree of hydrolysis） at 5%, pH 7.0, 20℃ and 15 min chelating time for FM （material not being defatted）. Four types of Fe-FPH including CA （deposit after chelating）, CB （deposit in 50% of absolute ethanol solution）, CC （suspended deposit in 80% of absolute ethanol solution）, and CD （bottom deposit in 80% of absolute ethanol solution） were fractionated with absolute ethanol from FM. Structural analysis through infra-red spectrum revealed that Fe^2＋ was combined strongly with amino-group and carboxyl-group in each chelate and each Fe^2＋ could form two five-member ring structures. All of the four chelates were shown more significant antioxidative activity and can be used as natural hydrophobic and hydrophilic antioxidant. Among all the chelates, the CB possesses the most effective antioxidative activity at 92% as high as that of a-tocopherol. Among all Fe-FPHs, only CD showed the most effective antibacterial activity against Escherichia coli, Staphylococcus aureus, Salmonella typhi, and Bacillus subtilis and can be used as natural antibacterial. It provides a more effective way for utilization of low value fish proteins and key information of Fe-FPH as additive in food industry.
文摘Mg-based alloys received significant attention for temporary implant applications while, their applications have been limited by high degradation rate. Therefore, silver-zeolite doped hydroxyapatite（Ag-Zeo-HAp） coating was synthesized on Ti O2-coated Mg alloy by physical vapour deposition（PVD） assisted electrodeposition technique to decrease the degradation rate of Mg alloy. X-ray diffraction（XRD） analysis and field emission scanning electron microscopy（FE-SEM） images showed the formation of a uniform and compact layer of Ag-Zeo-HAp with a thickness of 15 μm on the Ti O2 film with a thickness of 1 μm. The potentiodynamic polarization（PDP） and electrochemical impedance spectroscopy（EIS） tests indicated that corrosion resistance of Mg-Ca alloy was considerably increased by the Ag-Zeo-HAp coating. The bioactivity test in the simulated body fluid（SBF） solution showed that a dense and homogeneous bonelike apatite layer was formed on the Ag-Zeo-HAp surface after 14 d. Investigation of antibacterial activity via disk diffusion and spread plate methods showed that the Ag-Zeo-HAp coating had a significantly larger inhibition zone（3.86 mm） towards Escherichia coli（E. coli） compared with the Ti O2-coated Mg alloy（2.61 mm）. The Ag-Zeo-HAp coating showed high antibacterial performance, good bioactivity, and high corrosion resistance which make it a perfect coating material for biomedical applications.
文摘The work aims to characterize the structure and to evaluate in vitro the effect of different surface treatments on the bioactivity of medical grade Ti6Al7 Nb alloy implants manufactured by selective laser melting. In order to improve the bioactivity of these samples, they were subjected to heat treatment,chemical treatment, and impregnation with bioactive materials. To evaluate the apatite forming ability, the samples were immersed in simulated body fluid solution) and characterized before and after immersion by scanning electron microscopy, X-ray diffraction and X-ray photoelectron spectroscopy.The composition and the texture of the surfaces after the applied treatments have a selective effect on apatite layer development on the surface of samples.
