Objective:To investigate bioactive phytochemicals and antioxidant activities of Nymphaea nouchali and to explore its anticancer pathways by a network pharmacology approach.Methods:Using a spectrophotometer and high-pe...Objective:To investigate bioactive phytochemicals and antioxidant activities of Nymphaea nouchali and to explore its anticancer pathways by a network pharmacology approach.Methods:Using a spectrophotometer and high-performance liquid chromatography-diode array detector(HPLC-DAD),we quantified bioactive phytochemicals in methanolic extract of Nymphaea nouchali tuber.The extracts were investigated for in vitro antioxidant properties.Targets of these bioactive phytochemicals were predicted and anticancer-associated pathways were analyzed by a network pharmacology approach.Moreover,we identified the predicted genes associated with cancer pathways and the hub genes in the protein-protein interaction network of predicted genes.Results:Quantitative results indicated the total phenolics,total flavonoids,and total proanthocyanidins in the methanolic extract of Nymphaea nouchali tuber.HPLC-DAD analysis showed rutin(39.44 mg),catechin(39.20 mg),myricetin(30.77 mg),ellagic acid(11.05 mg),gallic acid(3.67 mg),vanillic acid(0.75 mg),rosmarinic acid(4.81 mg),p-coumaric acid(3.35 mg),and quercetin(0.90 mg)in 1 g of dry extract.The extract showed the radical scavenging activities of 2,2-diphenyl-1-picrylhydrazyl,2,2’-azinobis(3-ethylbenzothiazoline-6-sulfonic acid)and N,N-dimethyl-pphenylenediamine.By using network pharmacology,we predicted 130 target genes associated with cancer pathways.The top hub genes(IL6,AKT1,EGFR,JUN,PTGS2,MAPK3,CASP3,and CXCL8)were also identified,which were associated with cancer pathways and interacted with bioactive phytochemicals of the methanolic extract of Nymphaea nouchali tuber.Conclusions:Our study provides insights into the mechanism of anticancer activities of the methanolic extract of Nymphaea nouchali tuber.展开更多
We evaluated the antimutagenic effects kinds of bioactive phytochemicals and of 10 some phytochemical combinations against methotrexate （MTX）-induced genotoxicity by the umu test in Salmonella typhimurium TA1535/rpS...We evaluated the antimutagenic effects kinds of bioactive phytochemicals and of 10 some phytochemical combinations against methotrexate （MTX）-induced genotoxicity by the umu test in Salmonella typhimurium TA1535/rpSK1002 combined with a micronucleus assay. We observed that allicin, proanthocyanidins, polyphenols, eleutherosides, and isoflavones had higher antimutagenic activities than the other five types of bioactive phytochemicals. At the highest dose tested, MTX-induced genotoxicity was inhibited by 25%-75%. Kunming mice treated by MTX along with bioactive phytochemical combinations showed significant reduction in micronucleus induction and sperm abnormality rate （P〈0.01）. These results indicate that bioactive phytochemical combinations can be potentially used as new cytoprotectors.展开更多
Dillenia indica,commonly known as"elephant apple",is a tropical fruit-bearing tree with a rich history of medicinal use in traditional medicine across Southeast Asia and the Indian subcontinent.This review c...Dillenia indica,commonly known as"elephant apple",is a tropical fruit-bearing tree with a rich history of medicinal use in traditional medicine across Southeast Asia and the Indian subcontinent.This review comprehensively examines current research on the pharmacological properties of D.indica,focusing on its diverse bioactive compounds,including flavonoids,tannins,saponins,and triterpenoids,which underpin its key biological activities such as antioxidant,anti-inflammatory,antimicrobial,anticancer,antidiabetic,and hepatoprotective properties.Flavonoids and tannins exhibit potent antioxidant activity,effectively scavenging free radicals and reducing oxidative stress;a mechanism implicated in metabolic disorders,cancer,and neurodegenerative diseases.Additionally,the plant demonstrates considerable anti-inflammatory effects by modulating key inflammatory pathways,including the inhibition of proinflammatory cytokines such as tumor necrosis factor-a(TNF-a)and interleukin-6(IL-6).Saponins and triterpenoids enhance its antimicrobial activity,supporting traditional uses for treating infections.Preclinical studies indicate that D.indica extracts can inhibit cancer cell proliferation and induce apoptosis in various cancer types,including breast,colorectal,and liver cancers.Furthermore,specific formulations may improve the bioavailability and targeted delivery of active constituents,thereby increasing therapeutic efficacy.Despite the promising findings in vitro and in vivo,there is a notable lack of welldesigned clinical trials to validate these effects in humans.The plant is generally considered safe when consumed at recommended doses.However,excessive intake may lead to gastrointestinal discomfort and,in rare cases,hepatotoxicity.This review describes the therapeutic potential of D.indica as a natural agent while emphasizing the urgent need for comprehensive clinical trials,particularly those investigating pharmacodynamics profiles,to confirm its efficacy and safety.展开更多
基金carried out under the research and development(R and D)project of the Bangladesh Council of Scientific and Industrial Research
文摘Objective:To investigate bioactive phytochemicals and antioxidant activities of Nymphaea nouchali and to explore its anticancer pathways by a network pharmacology approach.Methods:Using a spectrophotometer and high-performance liquid chromatography-diode array detector(HPLC-DAD),we quantified bioactive phytochemicals in methanolic extract of Nymphaea nouchali tuber.The extracts were investigated for in vitro antioxidant properties.Targets of these bioactive phytochemicals were predicted and anticancer-associated pathways were analyzed by a network pharmacology approach.Moreover,we identified the predicted genes associated with cancer pathways and the hub genes in the protein-protein interaction network of predicted genes.Results:Quantitative results indicated the total phenolics,total flavonoids,and total proanthocyanidins in the methanolic extract of Nymphaea nouchali tuber.HPLC-DAD analysis showed rutin(39.44 mg),catechin(39.20 mg),myricetin(30.77 mg),ellagic acid(11.05 mg),gallic acid(3.67 mg),vanillic acid(0.75 mg),rosmarinic acid(4.81 mg),p-coumaric acid(3.35 mg),and quercetin(0.90 mg)in 1 g of dry extract.The extract showed the radical scavenging activities of 2,2-diphenyl-1-picrylhydrazyl,2,2’-azinobis(3-ethylbenzothiazoline-6-sulfonic acid)and N,N-dimethyl-pphenylenediamine.By using network pharmacology,we predicted 130 target genes associated with cancer pathways.The top hub genes(IL6,AKT1,EGFR,JUN,PTGS2,MAPK3,CASP3,and CXCL8)were also identified,which were associated with cancer pathways and interacted with bioactive phytochemicals of the methanolic extract of Nymphaea nouchali tuber.Conclusions:Our study provides insights into the mechanism of anticancer activities of the methanolic extract of Nymphaea nouchali tuber.
基金supported by the National Natural Science Foundation of China(Grant No.U1304307)the Young Core Instructor Foundation from the Education Commission of Henan Province,China(Grant No.2014GGJS-056)
文摘We evaluated the antimutagenic effects kinds of bioactive phytochemicals and of 10 some phytochemical combinations against methotrexate （MTX）-induced genotoxicity by the umu test in Salmonella typhimurium TA1535/rpSK1002 combined with a micronucleus assay. We observed that allicin, proanthocyanidins, polyphenols, eleutherosides, and isoflavones had higher antimutagenic activities than the other five types of bioactive phytochemicals. At the highest dose tested, MTX-induced genotoxicity was inhibited by 25%-75%. Kunming mice treated by MTX along with bioactive phytochemical combinations showed significant reduction in micronucleus induction and sperm abnormality rate （P〈0.01）. These results indicate that bioactive phytochemical combinations can be potentially used as new cytoprotectors.
基金the support from the European Regional Development Fund(Project ENOCH#CZ.02.1.01/0.0/0.0/16_019/0000868)The Czech Science Foundation(Project#23-05474S)。
文摘Dillenia indica,commonly known as"elephant apple",is a tropical fruit-bearing tree with a rich history of medicinal use in traditional medicine across Southeast Asia and the Indian subcontinent.This review comprehensively examines current research on the pharmacological properties of D.indica,focusing on its diverse bioactive compounds,including flavonoids,tannins,saponins,and triterpenoids,which underpin its key biological activities such as antioxidant,anti-inflammatory,antimicrobial,anticancer,antidiabetic,and hepatoprotective properties.Flavonoids and tannins exhibit potent antioxidant activity,effectively scavenging free radicals and reducing oxidative stress;a mechanism implicated in metabolic disorders,cancer,and neurodegenerative diseases.Additionally,the plant demonstrates considerable anti-inflammatory effects by modulating key inflammatory pathways,including the inhibition of proinflammatory cytokines such as tumor necrosis factor-a(TNF-a)and interleukin-6(IL-6).Saponins and triterpenoids enhance its antimicrobial activity,supporting traditional uses for treating infections.Preclinical studies indicate that D.indica extracts can inhibit cancer cell proliferation and induce apoptosis in various cancer types,including breast,colorectal,and liver cancers.Furthermore,specific formulations may improve the bioavailability and targeted delivery of active constituents,thereby increasing therapeutic efficacy.Despite the promising findings in vitro and in vivo,there is a notable lack of welldesigned clinical trials to validate these effects in humans.The plant is generally considered safe when consumed at recommended doses.However,excessive intake may lead to gastrointestinal discomfort and,in rare cases,hepatotoxicity.This review describes the therapeutic potential of D.indica as a natural agent while emphasizing the urgent need for comprehensive clinical trials,particularly those investigating pharmacodynamics profiles,to confirm its efficacy and safety.
