In this study, the effect of silica/calcium phosphate (SiCaP) nanocomposite particles on the properties of a novel chitosan-based thermosensitive hydrogel system was examined. SiCaP nanocomposite powder was fabricated...In this study, the effect of silica/calcium phosphate (SiCaP) nanocomposite particles on the properties of a novel chitosan-based thermosensitive hydrogel system was examined. SiCaP nanocomposite powder was fabricated using a sol-gel method and then used to fabricate nanocomposite hydrogels (Ch- <em>β</em>/7.5SiCaP and Ch-<em>β</em>/15SiCaP) including chitosan and <em>β</em>-glycerophosphate (Ch-<em>β</em>) as a matrix. Results revealed that compared to the Ch-<em>β </em>hydrogel without SiCaP, the presence of SiCaP particles in nanocomposite hydrogels maintained pH stability during the sol-gel transition, accelerated the gelation and improved the stiffness of nanocomposite hydrogels. Gelation time at 37℃ was reduced approximately 75% and stiffness was increased approximately 115%. Both of these changes are attributed to chemical and physical interactions of the SiCaP bioactive particles with chitosan. Furthermore, compared to the Ch-<em>β</em> hydrogel, the presence of SiCaP in the Ch-<em>β</em>/7.5SiCaP nanocomposite hydrogel did not affect biocompatibility negatively, but improved osteoblastic cell differentiation. Our studies suggest that these nanocomposite hydrogels may offer an innovative approach to bone regeneration strategies.展开更多
Mesoporous bioactive glasses have been widely investigated for applications in bone tissue regeneration and,more recently,in soft tissue repair and wound healing.In this study we produced mesoporous bioactive glass na...Mesoporous bioactive glasses have been widely investigated for applications in bone tissue regeneration and,more recently,in soft tissue repair and wound healing.In this study we produced mesoporous bioactive glass nanoparticles(MBGNs)based on the SiO2-CaO system.With the intention of adding subsidiary biological function,MBGNs were doped with Zn2+ions.Zn-MBGNs with 8 mol%ZnO content were synthesized via microemulsion assisted sol-gel method.The synthesized particles were homogeneous in shape and size.They exhibited spherical shape,good dispersity,and a size of 130±10 nm.The addition of zinc precursors did not affect the morphology of particles,while their specific surface area increased in comparison to MBGNs.The presence of Zn2+ions inhibited the formation of hydroxycarbonate apatite(HCAp)on the particles after immersion in simulated body fluid(SBF).No formation of HCAp crystals on the surface of Zn-MBGNs could be observed after 14 days of immersion.Interestingly,powders containing relatively high amount of zinc released Zn2+ions in low concentration(0.6-1.2 mg L^−1)but in a sustained manner.This releasing feature enables Zn-MBGNs to avoid potentially toxic levels of Zn2+ions,indeed Zn-MBGNs were seen to improve the differentiation of osteoblast-like cells(MG-63).Additionally,Zn-MBGNs showed higher ability to adsorb proteins in comparison to MBGNs,which could indicate a favourable later attachment of cells.Due to their advantageous morphological and physiochemical properties,Zn-MBGNs show great potential as bioactive fillers or drug delivery systems in a variety of applications including bone regeneration and wound healing.展开更多
In clinical trials,cell therapy,especially mesenchymal stem cells(MSC),contributed to wound treatment options in an innovative way.While,there are some limitations of MSC during the application process,including stric...In clinical trials,cell therapy,especially mesenchymal stem cells(MSC),contributed to wound treatment options in an innovative way.While,there are some limitations of MSC during the application process,including strict maintenance requirements and unpredictable differentiation.In order to expand the scope of application and reduce the restrictions related to direct use of SMC,the mechanisms of stem cells need to be studied.The latest research found that the effect of cell therapy is achieved mainly through paracrine effects,and the major bioactive vesicles which responsible for the paracrine effects is exosome.Exosome is a kind of extracellular membrane vesicle,which secreted from various cells and contain proteins,lipids and nucleic acids,to coordinate intercellular communication.In this review,the main cells type and cytokines relevant to every healing stage has been discussed,and the research about MSC-derived exosomes that has therapeutic effects has been summarized.At last,the potential application of exosomes as a bioactive material in the treatment of wound healing and related challenges as well as its possible solutions are discussed to reveal highly effective therapeutic strategies.展开更多
文摘In this study, the effect of silica/calcium phosphate (SiCaP) nanocomposite particles on the properties of a novel chitosan-based thermosensitive hydrogel system was examined. SiCaP nanocomposite powder was fabricated using a sol-gel method and then used to fabricate nanocomposite hydrogels (Ch- <em>β</em>/7.5SiCaP and Ch-<em>β</em>/15SiCaP) including chitosan and <em>β</em>-glycerophosphate (Ch-<em>β</em>) as a matrix. Results revealed that compared to the Ch-<em>β </em>hydrogel without SiCaP, the presence of SiCaP particles in nanocomposite hydrogels maintained pH stability during the sol-gel transition, accelerated the gelation and improved the stiffness of nanocomposite hydrogels. Gelation time at 37℃ was reduced approximately 75% and stiffness was increased approximately 115%. Both of these changes are attributed to chemical and physical interactions of the SiCaP bioactive particles with chitosan. Furthermore, compared to the Ch-<em>β</em> hydrogel, the presence of SiCaP in the Ch-<em>β</em>/7.5SiCaP nanocomposite hydrogel did not affect biocompatibility negatively, but improved osteoblastic cell differentiation. Our studies suggest that these nanocomposite hydrogels may offer an innovative approach to bone regeneration strategies.
基金This project has received funding from the European Union's Horizon 2020 research and innovation program under grant agreement No 739566 and in the frame of the project Centre for Functional and Surface Functionalized Glass(CEGLASS)ITMS code is 313011R453,operational program Research and innovation,co-funded from European Regional Development FundThe financial support of this work by the grant VEGA 2/0026/17 and APVV 15/0014 is also gratefully acknowledged.
文摘Mesoporous bioactive glasses have been widely investigated for applications in bone tissue regeneration and,more recently,in soft tissue repair and wound healing.In this study we produced mesoporous bioactive glass nanoparticles(MBGNs)based on the SiO2-CaO system.With the intention of adding subsidiary biological function,MBGNs were doped with Zn2+ions.Zn-MBGNs with 8 mol%ZnO content were synthesized via microemulsion assisted sol-gel method.The synthesized particles were homogeneous in shape and size.They exhibited spherical shape,good dispersity,and a size of 130±10 nm.The addition of zinc precursors did not affect the morphology of particles,while their specific surface area increased in comparison to MBGNs.The presence of Zn2+ions inhibited the formation of hydroxycarbonate apatite(HCAp)on the particles after immersion in simulated body fluid(SBF).No formation of HCAp crystals on the surface of Zn-MBGNs could be observed after 14 days of immersion.Interestingly,powders containing relatively high amount of zinc released Zn2+ions in low concentration(0.6-1.2 mg L^−1)but in a sustained manner.This releasing feature enables Zn-MBGNs to avoid potentially toxic levels of Zn2+ions,indeed Zn-MBGNs were seen to improve the differentiation of osteoblast-like cells(MG-63).Additionally,Zn-MBGNs showed higher ability to adsorb proteins in comparison to MBGNs,which could indicate a favourable later attachment of cells.Due to their advantageous morphological and physiochemical properties,Zn-MBGNs show great potential as bioactive fillers or drug delivery systems in a variety of applications including bone regeneration and wound healing.
基金funded by the Key Projects of the Joint Fund of the National Natural Science Foundation of China(U1804251)National Key Research and Development Program of China(2017YFB0702500,2018YFC1106703 and 2016YFC1102403)Innovation and Entrepreneurship Program for College Students(2021cxcy564).
文摘In clinical trials,cell therapy,especially mesenchymal stem cells(MSC),contributed to wound treatment options in an innovative way.While,there are some limitations of MSC during the application process,including strict maintenance requirements and unpredictable differentiation.In order to expand the scope of application and reduce the restrictions related to direct use of SMC,the mechanisms of stem cells need to be studied.The latest research found that the effect of cell therapy is achieved mainly through paracrine effects,and the major bioactive vesicles which responsible for the paracrine effects is exosome.Exosome is a kind of extracellular membrane vesicle,which secreted from various cells and contain proteins,lipids and nucleic acids,to coordinate intercellular communication.In this review,the main cells type and cytokines relevant to every healing stage has been discussed,and the research about MSC-derived exosomes that has therapeutic effects has been summarized.At last,the potential application of exosomes as a bioactive material in the treatment of wound healing and related challenges as well as its possible solutions are discussed to reveal highly effective therapeutic strategies.
