Precise recognition and specific interactions between biomolecules are key prerequisites for ensuring the performance of all actives within living organisms.The convergence of biomolecular recognition systems into syn...Precise recognition and specific interactions between biomolecules are key prerequisites for ensuring the performance of all actives within living organisms.The convergence of biomolecular recognition systems into synthetic materials could endow the materials with high specificity and biological sensitivity;this,in turn,enables precise drug release,monitoring or detection of important biomolecules,and cell manipulation through targeted capture or release of specific biomolecules.Meanwhile,from the perspective of materials science,the application of conventional polymers in practical biological systems poses several challenges,such as low responsiveness and sensitivity,inadequate targetability,insufficient anti-interference capacities,and unsatisfactory biocompatibility.These problems could be partly attributed to the polymers'weak discrimination abilities toward target biomolecules in the presence of interfering substances with high abundance.In particular,the proposition of“precision medicine”project raises higher demands for the design of biomaterials in terms of their precision and targetability.Therefore,there is an urgent demand for the development of new-generation biomaterials with precise recognition and sensitive responsiveness comparable to biomacro-molecules.This promotes a new research direction of biomolecule-responsive polymers and their diverse applications.This review focuses on the origin and construction of biomolecule-responsive polymers,as well as their attractive applications in drug delivery systems,bio-detection,bio-sensing,separation,and enrichment,as well as regulating cell adhesion.展开更多
Monitoring the intracellular adenosine triphosphate(ATP)level is vital for elaborating its function in physiological states.However,the intracellular fluorescence sensing of ATP using ATP aptamer remains difficult owi...Monitoring the intracellular adenosine triphosphate(ATP)level is vital for elaborating its function in physiological states.However,the intracellular fluorescence sensing of ATP using ATP aptamer remains difficult owing to non-target displacement and susceptibility toward enzymatic degradation.Herein,by combining 2D Cu-MOF nanosheets and FAM labelled ATP aptamer,we developed a fluorescent Cu-MOFs/aptamer nanoprobe to image and sense intracellular ATP.This nanoprobe reveals a very low fluorescence intensity because of the excellent quenching efficiency of 2D Cu-MOF nanosheets.The presence of ATP was capable to dissociate the FAM-aptamer from Cu-MOF nanosheets and resulted in an intense fluorescence signal.The Cu-MOFs/aptamer nanoprobe enables highly sensitive and selective measurement of ATP level ranging from 10μM to 800μM with a detection limit of 4.24μM.This nanosystem also further realized in situ detection of the undulation of ATP trigged by drug stimulation,depending on the selective delivery of the nanoprobe and attractive capability of resisting nonspecific displacement.The constructed nanoprobe may supply a potential platform in clinical diagnostics and biological studies.展开更多
Poly(α-L-lysine)(PLL)is a class of water-soluble,cationic biopolymer composed ofα-L-lysine structural units.The previous decade witnessed tremendous progress in the synthesis and biomedical applications of PLL and i...Poly(α-L-lysine)(PLL)is a class of water-soluble,cationic biopolymer composed ofα-L-lysine structural units.The previous decade witnessed tremendous progress in the synthesis and biomedical applications of PLL and its composites.PLL-based polymers and copolymers,till date,have been extensively explored in the contexts such as antibacterial agents,gene/drug/protein delivery systems,bio-sensing,bio-imaging,and tissue engineering.This review aims to summarize the recent advances in PLL-based nanomaterials in these biomedical fields over the last decade.The review first describes the synthesis of PLL and its derivatives,followed by the main text of their recent biomedical applications and translational studies.Finally,the challenges and perspectives of PLL-based nanomaterials in biomedical fields are addressed.展开更多
A highly sensitive and selective two-photon sensing scheme for detection of cartap was developed by using Au@Ag bimetallic core-shell nanoparticles.Cartap was found to induce the aggregation of Au@Ag nanoparticles and...A highly sensitive and selective two-photon sensing scheme for detection of cartap was developed by using Au@Ag bimetallic core-shell nanoparticles.Cartap was found to induce the aggregation of Au@Ag nanoparticles and up to 700-fold enhancement in two-photon photoluminescence.Huge enhancement in two-photon photoluminescence allows achieving a detection limit of as low as 0.0062 mg/kg,which is better than the conventional colorimetric methods.This two-photon sensing scheme has a broad dynamic range and displays excellent selectivity in detection of cartap against over other ten kinds of commonly used insecticides.展开更多
This study was conducted to identify promising applications of green silver nanoparticles(AgNPs)prepared from a bark extract of Sweetinia mahagoni(Sm).The green synthesized Sm-AgNPs were characterized using various sp...This study was conducted to identify promising applications of green silver nanoparticles(AgNPs)prepared from a bark extract of Sweetinia mahagoni(Sm).The green synthesized Sm-AgNPs were characterized using various spectroscopy methods.AgNPs were first investigated using ultraviolet-visible spectroscopy,and the metal nanoparticles exhibited an intense surfaceplasmon resonance(SPR)peak at different wavelengths.The green synthesized Sm-AgNPs had an SPR peak at 430 nm,which confirms the formation of Sm-AgNPs.In addition,Fourier transform infrared(FTIR)spectroscopy was conducted to determine the bioactive compounds of bark extract that actively participate in the reduction of Sm-AgNPs,and the results revealed O−H stretching of free hydroxyl alcohol and phenols,N−H bonds of primary amines,S=O stretching of sulfoxide in aromatic groups,C−I stretching due to aliphatic iodo compounds,and C−Br stretching by halo compounds of the bark extract which might reduce and stabilize Sm-AgNPs.Scanning electron microscopy(SEM)and energy dispersive X-ray spectroscopy(EDS)results revealed that Sm-AgNPs were approximately irregular spheres.EDS results revealed the complete reduction of silver to elemental silver.The particle size analysis of Sm-AgNPs was conducted using dynamic light scattering(DLS),and the results revealed that Sm-AgNPs were polydisperse with an average size range from 35.8 to 47.8 nm,an average mean size of 41.3 nm,and a Z average of 37.7 nm.Sm-AgNPs had a negative zeta potential value of−19.0 mV,indicating that Sm-AgNPs were very stable in colloidal form.Further studies were carried out to demonstrate their usefulness in industrial and biomedical applications.In these studies,Sm-AgNPs exhibited a very good antibacterial activity against both Gram-negative and Gram-positive bacteria.In addition to regular assays,we also investigated important industrial applications such as the reduction of toxic hexavalent chromium to a nontoxic form and sensing of Hg2+ions.The results revealed that Sm-AgNPs had an excellent performance in biosensor applications such as sensing and detecting mercury at parts per million/parts per billion levels.In conclusion,green Sm-AgNPs are promising materials in therapeutic and industrial applications.展开更多
We report the extraordinary result of rapid fibre Bragg grating inscription in doped polymer optical fibres based on polymethyl methacrylate in only 7 ms,which is two orders of magnitude faster than the inscription ti...We report the extraordinary result of rapid fibre Bragg grating inscription in doped polymer optical fibres based on polymethyl methacrylate in only 7 ms,which is two orders of magnitude faster than the inscription times previously reported.This was achieved using a new dopant material,diphenyl disulphide,which was found to enable a fast,positive refractive index change using a low ultraviolet dose.These changes were investigated and found to arise from photodissociation of the diphenyl disulphide molecule and subsequent molecular reorganization.We demonstrate that gratings inscribed in these fibres can exhibit at least a 15 times higher sensitivity than silica glass fibre,despite their quick inscription times.As a demonstration of the sensitivity,we selected a highly stringent situation,namely,the monitoring of a human heartbeat and respiratory functions.These findings could permit the inscription of fibre Bragg gratings during the fibre drawing process for mass production,allowing costeffective,single-use,in vivo sensors among other potential uses.展开更多
基金National Key Research and Development Program of China,Grant/Award Number:2022YFC3400800National Natural Science Foundation of China,Grant/Award Numbers:22004120,22174138,22104013+2 种基金DICP Innovation Funding,Grant/Award Numbers:I202243,I202229Dalian Outstanding Young Scientific Talent,Grant/Award Number:2020RJ01Natural Science Foundation of Jiangxi Province,Grant/Award Numbers:20212BAB213002,20232BAB213049。
文摘Precise recognition and specific interactions between biomolecules are key prerequisites for ensuring the performance of all actives within living organisms.The convergence of biomolecular recognition systems into synthetic materials could endow the materials with high specificity and biological sensitivity;this,in turn,enables precise drug release,monitoring or detection of important biomolecules,and cell manipulation through targeted capture or release of specific biomolecules.Meanwhile,from the perspective of materials science,the application of conventional polymers in practical biological systems poses several challenges,such as low responsiveness and sensitivity,inadequate targetability,insufficient anti-interference capacities,and unsatisfactory biocompatibility.These problems could be partly attributed to the polymers'weak discrimination abilities toward target biomolecules in the presence of interfering substances with high abundance.In particular,the proposition of“precision medicine”project raises higher demands for the design of biomaterials in terms of their precision and targetability.Therefore,there is an urgent demand for the development of new-generation biomaterials with precise recognition and sensitive responsiveness comparable to biomacro-molecules.This promotes a new research direction of biomolecule-responsive polymers and their diverse applications.This review focuses on the origin and construction of biomolecule-responsive polymers,as well as their attractive applications in drug delivery systems,bio-detection,bio-sensing,separation,and enrichment,as well as regulating cell adhesion.
基金supported in part by the National Natural Science Foundation of China(21974125 and 21605038)the National 111 Project of China(D20003)the Collaborative Innovation Project of Zhengzhou(Zhengzhou University)(Grant No.18XTZX12002)
文摘Monitoring the intracellular adenosine triphosphate(ATP)level is vital for elaborating its function in physiological states.However,the intracellular fluorescence sensing of ATP using ATP aptamer remains difficult owing to non-target displacement and susceptibility toward enzymatic degradation.Herein,by combining 2D Cu-MOF nanosheets and FAM labelled ATP aptamer,we developed a fluorescent Cu-MOFs/aptamer nanoprobe to image and sense intracellular ATP.This nanoprobe reveals a very low fluorescence intensity because of the excellent quenching efficiency of 2D Cu-MOF nanosheets.The presence of ATP was capable to dissociate the FAM-aptamer from Cu-MOF nanosheets and resulted in an intense fluorescence signal.The Cu-MOFs/aptamer nanoprobe enables highly sensitive and selective measurement of ATP level ranging from 10μM to 800μM with a detection limit of 4.24μM.This nanosystem also further realized in situ detection of the undulation of ATP trigged by drug stimulation,depending on the selective delivery of the nanoprobe and attractive capability of resisting nonspecific displacement.The constructed nanoprobe may supply a potential platform in clinical diagnostics and biological studies.
基金This work was financially supported by the National Natural Science Foundation of China,China(No.81803467)2020 Li Ka Shing Foundation Cross-Disciplinary Research Grant,Hong Kong(2020LKSFG18B,2020LKSFG02E)the grant for Key Disciplinary Project of Clinical Medicine under the Guangdong High-Level University Development Program,Guangdong,China(002-18120314,002-18120311).
文摘Poly(α-L-lysine)(PLL)is a class of water-soluble,cationic biopolymer composed ofα-L-lysine structural units.The previous decade witnessed tremendous progress in the synthesis and biomedical applications of PLL and its composites.PLL-based polymers and copolymers,till date,have been extensively explored in the contexts such as antibacterial agents,gene/drug/protein delivery systems,bio-sensing,bio-imaging,and tissue engineering.This review aims to summarize the recent advances in PLL-based nanomaterials in these biomedical fields over the last decade.The review first describes the synthesis of PLL and its derivatives,followed by the main text of their recent biomedical applications and translational studies.Finally,the challenges and perspectives of PLL-based nanomaterials in biomedical fields are addressed.
基金the Ministry of Education, Singapore (R-143-000-607-112)the National Research Foundation, Prime Minister’s Office, Singapore under its Competitive Research Program (NRF-CRP10-2012-04)Medium Sized Centre Programme
文摘A highly sensitive and selective two-photon sensing scheme for detection of cartap was developed by using Au@Ag bimetallic core-shell nanoparticles.Cartap was found to induce the aggregation of Au@Ag nanoparticles and up to 700-fold enhancement in two-photon photoluminescence.Huge enhancement in two-photon photoluminescence allows achieving a detection limit of as low as 0.0062 mg/kg,which is better than the conventional colorimetric methods.This two-photon sensing scheme has a broad dynamic range and displays excellent selectivity in detection of cartap against over other ten kinds of commonly used insecticides.
文摘This study was conducted to identify promising applications of green silver nanoparticles(AgNPs)prepared from a bark extract of Sweetinia mahagoni(Sm).The green synthesized Sm-AgNPs were characterized using various spectroscopy methods.AgNPs were first investigated using ultraviolet-visible spectroscopy,and the metal nanoparticles exhibited an intense surfaceplasmon resonance(SPR)peak at different wavelengths.The green synthesized Sm-AgNPs had an SPR peak at 430 nm,which confirms the formation of Sm-AgNPs.In addition,Fourier transform infrared(FTIR)spectroscopy was conducted to determine the bioactive compounds of bark extract that actively participate in the reduction of Sm-AgNPs,and the results revealed O−H stretching of free hydroxyl alcohol and phenols,N−H bonds of primary amines,S=O stretching of sulfoxide in aromatic groups,C−I stretching due to aliphatic iodo compounds,and C−Br stretching by halo compounds of the bark extract which might reduce and stabilize Sm-AgNPs.Scanning electron microscopy(SEM)and energy dispersive X-ray spectroscopy(EDS)results revealed that Sm-AgNPs were approximately irregular spheres.EDS results revealed the complete reduction of silver to elemental silver.The particle size analysis of Sm-AgNPs was conducted using dynamic light scattering(DLS),and the results revealed that Sm-AgNPs were polydisperse with an average size range from 35.8 to 47.8 nm,an average mean size of 41.3 nm,and a Z average of 37.7 nm.Sm-AgNPs had a negative zeta potential value of−19.0 mV,indicating that Sm-AgNPs were very stable in colloidal form.Further studies were carried out to demonstrate their usefulness in industrial and biomedical applications.In these studies,Sm-AgNPs exhibited a very good antibacterial activity against both Gram-negative and Gram-positive bacteria.In addition to regular assays,we also investigated important industrial applications such as the reduction of toxic hexavalent chromium to a nontoxic form and sensing of Hg2+ions.The results revealed that Sm-AgNPs had an excellent performance in biosensor applications such as sensing and detecting mercury at parts per million/parts per billion levels.In conclusion,green Sm-AgNPs are promising materials in therapeutic and industrial applications.
基金supported by the General Research Fund Project(n1 PolyU 152207/15E)PolyU Central Grant project 1-ZVGBsupport from the Hong Kong Research Grants Council through the Hong Kong PhD Fellowship Scheme.
文摘We report the extraordinary result of rapid fibre Bragg grating inscription in doped polymer optical fibres based on polymethyl methacrylate in only 7 ms,which is two orders of magnitude faster than the inscription times previously reported.This was achieved using a new dopant material,diphenyl disulphide,which was found to enable a fast,positive refractive index change using a low ultraviolet dose.These changes were investigated and found to arise from photodissociation of the diphenyl disulphide molecule and subsequent molecular reorganization.We demonstrate that gratings inscribed in these fibres can exhibit at least a 15 times higher sensitivity than silica glass fibre,despite their quick inscription times.As a demonstration of the sensitivity,we selected a highly stringent situation,namely,the monitoring of a human heartbeat and respiratory functions.These findings could permit the inscription of fibre Bragg gratings during the fibre drawing process for mass production,allowing costeffective,single-use,in vivo sensors among other potential uses.
