Cation-exchange chromatography was selected to extract and separate betaines from marine algae. On the basis of the special chemical characteristics of the betaines and their analogues, Dragendorff’s reagent (KBiI 4)...Cation-exchange chromatography was selected to extract and separate betaines from marine algae. On the basis of the special chemical characteristics of the betaines and their analogues, Dragendorff’s reagent (KBiI 4) was used to test the existence of betaines and their analogues in marine algae. The total content of betaines from seven species was obtained by using the Reinkeate salt precipitation method. The results showed that the content of betaines in two species of Chlorophyta and two species of Rhodophyta were relatively high,and that the content of betaines in Enteromopha prolifera could even reach to 0.9%. The content in the three species of Phaeophyta was relatively low.展开更多
Five new zwitterionic surfactants with long chain alkyl betaine structure incorporated with hydroxylpropyl group have been synthesized. Their structures were identified by elemental analysis, IR (HNMR)-H-1, and (CNMR)...Five new zwitterionic surfactants with long chain alkyl betaine structure incorporated with hydroxylpropyl group have been synthesized. Their structures were identified by elemental analysis, IR (HNMR)-H-1, and (CNMR)-C-13. Surface tension experiments showed that these surfactants have higher surface activity than those without hydroxypropyl group. The values of CMC and gamma(CMC) of these surfactants have been determined.展开更多
Pyridinium ylids 4 were generated as reaction intermediates from the decarboxylation of pyridinium betaines 3, which were prepared from the reactions of a-amino acid ester hydrochlorides with 2, 4, 6-triphenylpyrylium...Pyridinium ylids 4 were generated as reaction intermediates from the decarboxylation of pyridinium betaines 3, which were prepared from the reactions of a-amino acid ester hydrochlorides with 2, 4, 6-triphenylpyrylium tetrafluoroborate. Protonation, addition and substitution reactions of 4 with electrophiles were studied in this paper.展开更多
Good’s buffers have been widely applied in cell/organ culture over the past half a century as biocompatible pH stabilizers.However,the emergence of severe adverse effects,such as cellular uptake,lysosomal autophagic ...Good’s buffers have been widely applied in cell/organ culture over the past half a century as biocompatible pH stabilizers.However,the emergence of severe adverse effects,such as cellular uptake,lysosomal autophagic activation,and visible light-induced cytotoxicity,raises serious questions over its biocompatibility while underlying mechanism was unclear.Here we report that riboflavin(RF,component of cell culture medium)generates ^(1)O_(2),⋅OH,and O_(2)^(·-)under visible light exposure during regular cell manipulation.These short half-life reactive oxygen species(ROS)react with tertiary amine groups of HEPES,producing 106.6μM of H_(2)O_(2).Orders of magnitude elevated half-life of ROS in the medium caused severe cytotoxicity and systematic disorder of normal cell functions.We have further designed and validated zwitterionic betaines as the new generation biocompatible organic pH buffers,which is able to completely avoid the adverse effects that found on HEPES and derivate Good’s buffers.These findings may also open a new avenue for zwitterionic betaine based materials for biomedical applications.展开更多
Plant-related organic compound(PROC)may interact with redox-active metals like iron while they are present in soil or aquatic environment,but their effects on the photoreduction of Fe(III)remain largely unexplored.Thi...Plant-related organic compound(PROC)may interact with redox-active metals like iron while they are present in soil or aquatic environment,but their effects on the photoreduction of Fe(III)remain largely unexplored.This study investigates the photochemical behavior of Fe(III)-PROC complexes using alkaline lignin(AL),betaine hydrochloride(BH),and phytic acid(PA)as representative proxies for PROC.The reductive agent AL demonstrated the ability to directly reduce Fe(III)to Fe(II).In contrast,BH,being unable to form strong complexes with Fe(III),was able to quench·OH,thereby resulting in a shift of the redox equilibrium towards Fe(II).PA exhibited a strong binding affinity for Fe(III),effectively inhibiting its photoreduction.Electron paramagnetic resonance(EPR)analysis,utilizing 5,5-dimethyl-1-pyrroline-N-oxide(DMPO)as a spin trap,revealed that the DMPO-OH signal detected in photolyzed Fe(III)-PROC solutions originated from various pathways.Specifically,uncomplexed Fe(III)in AL or BH solutions was shown to oxidize DMPO directly,leading to the formation of a false DMPO-OH adduct.The addition of ethanol to the photolyzed Fe(III)-AL and Fe(III)-BH systems resulted in the generation of the DMPO-CH(CH_(3))OH adduct,thereby confirming the presence of authentic·OH in these systems.The photolysis of the Fe(III)-PA complex may proceed via a photodissociation mechanism,where the resulting loosely bound Fe(III)can oxidize DMPO,followed by a nucleophilic attack from water.This research highlights the multifaceted roles of PROC in facilitating the redox cycling of iron within soil and aquatic ecosystems.展开更多
Exercise,as a non-pharmacological health intervention,has been widely recognized for its beneficial effects,yet its underlying molecular mechanisms remain incompletely understood.The duration,frequency,and intensity o...Exercise,as a non-pharmacological health intervention,has been widely recognized for its beneficial effects,yet its underlying molecular mechanisms remain incompletely understood.The duration,frequency,and intensity of exercise exert distinct physiological impacts on the human body[1].Notably,acute exercise(AE)primarily elicits immediate metabolic responses and immune activation to cope with environmental stimuli,whereas long-term exercise(LE)induces cumulative health benefits across multiple organ systems[2‒4].Aging represents a complex biological process that persists throughout the ontogenetic continuum and serves as a pivotal etiological determinant for numerous chronic pathologies.In the context of accelerating global demographic aging,the development of interventions to promote healthspan extension and modulate aging trajectories has become a paramount research imperative in geroscience.Currently,research on the relationship between exercise and aging is a hot topic.For example,exercise has been shown to modulate aging through pathways such as AMP-activated protein kinase(AMPK)[5].However,the precise molecular links remain elusive.In a recent breakthrough study,Geng et al.used a novel multi-omics strategy to pinpoint betaine,a glycine derivative from choline/diet that serves as both a hepatic methyl donor and a renal osmoprotectant,as a key exercise-induced molecule with anti-inflammatory and geroprotective effects mediated partially via TANK-binding kinase 1(TBK1)inhibition[6,7].This work represents a significant advance as it systematically maps the molecular divergence between acute and long-term exercise while establishing a direct link between renal metabolism and systemic senescence-delaying benefits.展开更多
BACKGROUND Skin wounds are highly common in diabetic patients,and with increasing types of pathogenic bacteria and antibiotic resistance,wounds and infections in diabetic patients are difficult to treat and heal.AIM T...BACKGROUND Skin wounds are highly common in diabetic patients,and with increasing types of pathogenic bacteria and antibiotic resistance,wounds and infections in diabetic patients are difficult to treat and heal.AIM To explore the effects of betaine ointment(BO)in promoting the healing of skin wounds and reducing the inflammation and apoptosis of skin cells in microbially infected diabetic mice.METHODS By detecting the minimum inhibitory concentrations(MICs)of betaine and plant monomer components such as psoralen,we prepared BO with betaine as the main ingredient,blended it with traditional Chinese medicines such as gromwell root and psoralen,and evaluated its antibacterial effects and safety in vitro and in vivo.The skin infection wound models of ordinary mice and diabetic mice were constructed,and the OTC drugs mupirocin ointment and Zicao ointment were used as controls to evaluate the antibacterial effects in vivo and the anti-inflammatory and anti-apoptotic effects of BO.RESULTS The MICs of betaine against microorganisms such as Staphylococcus aureus(S.aureus),Candida albicans and Cryptococcus neoformans ranged from 4 to 32μg/mL.Gromwell root and psoralea,both of which contain antimicrobial components,mixed to prepare BO with MICs ranging from 16 to 64μg/mL,which is 32-256 times lower than those of Zicao ointment,although the MIC is greater than that of betaine.After 15 days of treatment with BO for USA300-infected ordinary mice,the wound scab removal rates were 83.3%,while those of mupirocin ointment and Zicao ointment were 66.7%and 0%,respectively,and the differences were statistically significant.In diabetic mice,the wound scab removal rate of BO and mupirolacin ointment was 80.0%,but BO reduced wound inflammation and the apoptosis of skin cells and facilitated wound healing.CONCLUSION The ointment prepared by mixing betaine and traditional Chinese medicine can effectively inhibit common skin microorganisms and has a strong effect on the skin wounds of sensitive or drug-resistant S.aureus-infected ordinary mice and diabetic mice.展开更多
The electrodeposition behaviors of nickel on glassy carbon（GC） and carbon steel（CS） electrodes were investigated in the14.3%-85.7%（mole fraction） betaine.HCl ethylene glycol（EG） ionic liquid using cyclic volta...The electrodeposition behaviors of nickel on glassy carbon（GC） and carbon steel（CS） electrodes were investigated in the14.3%-85.7%（mole fraction） betaine.HCl ethylene glycol（EG） ionic liquid using cyclic voltammetry and chronoamperometry.The results indicated that the reduction of Ni（Ⅱ） on CS electrode via a diffusion-controlled quasi-reversible process was much more facile and easier than that occurred on GC electrode,which followed a diffusion-controlled three-dimensional instantaneous nucleation and growth.Scanning electron microscopy was used to observe that the deposit was dense and contained fine crystallites with average size of（80±4） nm.Energy dispersive spectrometer analysis showed that the obtained deposit was metallic nickel.X-ray diffraction spectroscopy indicated that（111） plane was the most preferred crystal orientation.The nickel deposit was luminous and bright,and had good adhesion with the CS substrate.展开更多
[Objective] The research aimed to discuss the relationship between Glycine betaine and the salt-tolerance mechanism of different pumpkins and confirm the mechanism of betaine in salt-tolerance physiology.[Method] Taki...[Objective] The research aimed to discuss the relationship between Glycine betaine and the salt-tolerance mechanism of different pumpkins and confirm the mechanism of betaine in salt-tolerance physiology.[Method] Taking the seedlings of C.ficifolia and Qingli pumpkin as test materials,the effects of glycine betaine on the cell membrane permeability,MDA and root activity of two varieties of pumpkin seedlings under 300 mmol/L NaCl stress were studied.[Result] At suitable concentrations,glycine betaine could d...展开更多
以盐酸甜菜碱(Betaine·HCl)、乙二醇(EG)和六水合硫酸镍(NiSO_4·6H_2O)制备的Betaine·HCl-6EG-nNiSO_4·6H_2O低共熔离子液体为电解液,在碳钢(CS)基体上电沉积出平整致密的金属镍镀层.采用旋转黏度计和电导率仪分别...以盐酸甜菜碱(Betaine·HCl)、乙二醇(EG)和六水合硫酸镍(NiSO_4·6H_2O)制备的Betaine·HCl-6EG-nNiSO_4·6H_2O低共熔离子液体为电解液,在碳钢(CS)基体上电沉积出平整致密的金属镍镀层.采用旋转黏度计和电导率仪分别测定了其黏度和电导率.在测试条件下,其黏度为3.918~55.19 m Pa·s,电导率为1.109~0.199 S/m.循环伏安测试结果表明Betaine·HCl-6EG-nNiSO_4·6H_2O(n=0.071)低共熔离子液体中Ni^(2+)在CS电极上的电化学还原过程可以描述为Ni^(2+)→Ni^0,其起始还原电位为-0.56 V.对CS基体上的沉积层进行XRD和EDAS测试,分析结果表明镀层为具有面心立方晶型结构的金属镍.SEM结果表明得到的镍镀层晶粒细小致密且均匀.镀层截面组织金相照片和线性扫描能谱图表明沿CS基体各处均得到厚度分布均匀且与基体结合良好的镍镀层,其厚度约为11μm.展开更多
[Objective] This study aimed to analyze the effects of different concentrations of glycine betaine（GB） on oxidation metabolism in cucumbers under low-temperature stress and to investigate the possible mechanism of l...[Objective] This study aimed to analyze the effects of different concentrations of glycine betaine（GB） on oxidation metabolism in cucumbers under low-temperature stress and to investigate the possible mechanism of low-temperature resistance in cucumber during low-temperature storage. [Method] Cucumber cultivar Zhongnong No.8 was treated with 0, 5, 10 and 15 mmol/L GB solutions for 15 min and stored at 4 ℃. Changes in oxidative metabolism-related parameters were observed. [Result] Increasing exogenous GB concentration could enhance GB content in cucumbers, decline lipoxygenase（LOX） activity, improve peroxidase（POD） and catalase（CAT） activities, remove effectively hydrogen peroxide（H2O2） and reduce the accumulation of malondialdehyde（MDA）. [Conclusion] Treating cucumbers with10 mmol/L GB exhibited the most remarkable effect.展开更多
1, 3 Dipolar cycloaddition reaction between vinyl acetate and 3-hydroxypyridiniun betaine was performed under solid-liquid phase transfer catalytic condition, This reaction has been successfully used on the synthesis ...1, 3 Dipolar cycloaddition reaction between vinyl acetate and 3-hydroxypyridiniun betaine was performed under solid-liquid phase transfer catalytic condition, This reaction has been successfully used on the synthesis of an analogue of Bao-Gong-Teng A.展开更多
AIM:To test whether ethanol feeding could induce Toll-like receptor 4(TLR4)responses,assess the hepatoprotective effect of betaine and its inhibitive effect on TLR4 in animal models of alcoholic liver injury.METHODS:F...AIM:To test whether ethanol feeding could induce Toll-like receptor 4(TLR4)responses,assess the hepatoprotective effect of betaine and its inhibitive effect on TLR4 in animal models of alcoholic liver injury.METHODS:Forty-eight female Sprague-Dawley rats were randomly divided into four groups as control,model,low and high dose betaine groups.Except control group,all rats were fed with high fat-containing diet plus ethanol and fish oil gavages for 8 wk.Betaine was administered intragastrically after exposure of ethanol for 4 wk.The changes of liver histology were examined.The expression of TLR4 mRNA and protein was detected by RT-PCR and Western blotting,respectively.The serum aminotransferase activity alanine transarninase(ALT),aspartate aminotransferase(AST),serum endotoxin,and liver inflammatory factors tumor necrosis factor-α(TNF-α),interferon-γ(IFN-γ),interleukin-18(IL-18)were also assayed.RESULTS:Compared with control group,rats of model group developed marked liver injury,accompanied by an increase of ALT(159.41±7.74 U/L vs 59.47± 2.34 U/L,P<0.0001),AST(248.25±1.40 U/L vs 116.89±3.48 U/L,P<0.0001),endotoxin(135.37± 30.17 ng/L vs 44.15±7.54 ng/L,P<0.0001),TNF-α(20.81±8.58 pg/mL vs 9.34±2.57 pg/mL,P=0.0003),IFN-γ(30.18±7.60 pg/mL vs 16.86±9.49 pg/mL,P= 0.0039)and IL-18(40.99±8.25 pg/mL vs 19.73±9.31 pg/mL,P=0.0001).At the same time,the expression of TLR4 mRNA and protein was markedly induced in the liver after chronic ethanol consumption(1.45±0.07 vs 0.44±0.04,P<0.0001;1.83±0.13 vs 0.56±0.08,P<0.0001).Compared with model group,betaine feeding resulted in significant decreases of ALT(64.93 ±6.06 U/L vs 159.41±7.74 U/L,P<0.0001),AST(188.73±1.11 U/L vs 248.25±1.40 U/L,P<0.0001),endotoxin(61.80±12.56 ng/L vs 135.37±30.17 ng/L,P<0.0001),TNF-α(9.79±1.32 pg/mL vs 20.81± 8.58 pg/mL,P=0.0003),IFN-γ(18.02±5.96 pg/mL vs 30.18±7.60 pg/mL,P=0.0008)and IL-18(18.23±7.01 pg/mL vs 40.99±8.25 pg/mL,P<0.0001).Betaine also improved liver steatosis.The expression levels of TLR4 mRNA or protein in liver tissues were significantly lowered(0.62±0.04 vs 1.45±0.07,P<0.0001;and 0.65±0.06 vs 1.83±0.13,P<0.0001).There was a statistical difference of TLR4 mRNA and protein expression between high-and low-dose betaine groups(0.62±0.04 vs 0.73±0.05,P<0.0001,and 0.65±0.06 vs 0.81±0.09,P<0.0001).CONCLUSION:Betaine can prevent the alcoholinduced liver injury effectively and improve the liver function.The expression of TLR4 increases significantly in ethanol-fed rats and betaine administration can inhibit TLR4 expression.展开更多
Plants synthesize the osmoprotectant glycine betaine (GB) via choline→betaine aldehyde→glycine be- taine[1]. Two enzymes are involved in the pathway choline monooxygenase (CMO) and betaine aldehyde dehydrogenase (BA...Plants synthesize the osmoprotectant glycine betaine (GB) via choline→betaine aldehyde→glycine be- taine[1]. Two enzymes are involved in the pathway choline monooxygenase (CMO) and betaine aldehyde dehydrogenase (BADH). A full length CMO cDNA (1,643bp) was cloned from Amaranthus tricolor. The open reading frame encoded a 442-amino acid polypeptide, which showed 69% identity with CMOs in Spina- cia oleracea L. and Beta vulgaris L. DNA gel blot analysis indicated the presence of one copy of CMO gene in the A. tricolor genome. The expressions of CMO and BADH proteins in A.tricolor leaves significantly increased under salinization, drought and heat stress (42℃), as determined by immunoblot analysis, but did not respond to cold stress (4℃), or exogenous ABA application. The increase of GB content in leaves was parallel to CMO and BADH contents.展开更多
Glycinebetaine （Glybet） accumulation, photosynthetic efficiency and growth performance in indica rice cultivated under salt stress and extreme pH stress were investigated. Betaine aldehyde dehydrogenase （BADH） act...Glycinebetaine （Glybet） accumulation, photosynthetic efficiency and growth performance in indica rice cultivated under salt stress and extreme pH stress were investigated. Betaine aldehyde dehydrogenase （BADH） activity and Glybet accumulation in the seedlings of salt-tolerant and salt-sensitive rice varieties grown under saline and acidic conditions peaked after treatment for 72 h and 96 h, respectively, and were higher than those grown under neutral pH and alkaline salt stress. A positive correlation was found between BADH activity and Glybet content in both salt-tolerant （P=0.71） and salt-sensitive （P=0.86） genotypes. The chlorophyll a, chlorophyll b, total chlorophyll and total carotenoids contents in the stressed seedlings significantly decreased under both acidic and alkaline stresses, especially in the salt-sensitive genotype. Similarly, the maximum quantum yield of PSII （Fv/Fm）, photon yield of PSII （ФPSII）, non-photochemical quenching （NPQ） and net photosynthetic rate （Pn） in the stressed seedlings were inhibited, leading to overall growth reduction. The positive correlations between chlorophyll a content and Fv/Fm, total chlorophyll content and ФPSII, and Pn as well as Pn and leaf area in both salt-tolerant and salt-sensitive genotypes were found. Saline acidic and saline alkaline soils may play a key role affecting vegetative growth prior to the reproductive stage in rice plants.展开更多
Background： Betaine affects fat metabolism in animals, but the specific mechanism is still not clear. The purpose of this study was to investigate possible mechanisms of betaine in altering lipid metabolism in muscle...Background： Betaine affects fat metabolism in animals, but the specific mechanism is still not clear. The purpose of this study was to investigate possible mechanisms of betaine in altering lipid metabolism in muscle tissue in finishing pigs.Methods： A total of 120 crossbred gilts（Landrace × Yorkshire × Duroc） with an average initial body weight of 70.1 kg were randomly allotted to three dietary treatments. The treatments included a corn–soybean meal basal diet supplemented with 0, 1250 or 2500 mg/kg betaine. The feeding experiment lasted 42 d.Results： Betaine addition to the diet significantly increased the concentration of free fatty acids（FFA） in muscle（P 〈 0.05）. Furthermore, the levels of serum cholesterol and high-density lipoprotein cholesterol were decreased（P 〈 0.05） and total cholesterol content was increased in muscle（P 〈 0.05） of betaine fed pigs. Experiments on genes involved in fatty acid transport showed that betaine increased expression of lipoprotein lipase（LPL）, fatty acid translocase/cluster of differentiation（FAT/CD36）, fatty acid binding protein（FABP3） and fatty acid transport protein（FATP1）（P 〈 0.05）. The abundance of fatty acid transport protein and fatty acid binding protein were also increased by betaine（P 〈 0.05）. As for the key factors involved in fatty acid oxidation, although betaine supplementation didn＇t affect the level of carnitine and malonyl-CoA, betaine increased mR NA and protein abundance of carnitine palmitransferase-1（CPT1）and phosphorylated-AMPK（P 〈 0.05）.Conclusions： The results suggested that betaine may promoted muscle fatty acid uptake via up-regulating the genes related to fatty acid transporter including FAT/CD36, FATP1 and FABP3. On the other hand, betaine activated AMPK and up-regulated genes related to fatty acid oxidation including PPARα and CPT1. The underlying mechanism regulating fatty acid metabolism in pigs supplemented with betaine is associated with the up-regulation of genes involved in fatty acid transport and fatty acid oxidation.展开更多
Nonalcoholic steatohepatitis (NASH) is an important indication for liver transplantation in many Western countries. Obesity and insulin resistance are the two most common risk factors for NASH, which can lead to recur...Nonalcoholic steatohepatitis (NASH) is an important indication for liver transplantation in many Western countries. Obesity and insulin resistance are the two most common risk factors for NASH, which can lead to recurrent NASH after liver transplantation. There is currently no approved therapy for NASH, and treatment is directed at risk factor modification and lifestyle changes. Betaine has been used for NASH, with mixed results, and may show promise in conjunction with other agents in clinical trials.展开更多
文摘Cation-exchange chromatography was selected to extract and separate betaines from marine algae. On the basis of the special chemical characteristics of the betaines and their analogues, Dragendorff’s reagent (KBiI 4) was used to test the existence of betaines and their analogues in marine algae. The total content of betaines from seven species was obtained by using the Reinkeate salt precipitation method. The results showed that the content of betaines in two species of Chlorophyta and two species of Rhodophyta were relatively high,and that the content of betaines in Enteromopha prolifera could even reach to 0.9%. The content in the three species of Phaeophyta was relatively low.
文摘Five new zwitterionic surfactants with long chain alkyl betaine structure incorporated with hydroxylpropyl group have been synthesized. Their structures were identified by elemental analysis, IR (HNMR)-H-1, and (CNMR)-C-13. Surface tension experiments showed that these surfactants have higher surface activity than those without hydroxypropyl group. The values of CMC and gamma(CMC) of these surfactants have been determined.
基金We want to express our sincere appreciation to Professor Joe Wilson of University of Kentucky and Professor Xiulin Ye of Peking University for their favorable suggestions and assistance for the work.
文摘Pyridinium ylids 4 were generated as reaction intermediates from the decarboxylation of pyridinium betaines 3, which were prepared from the reactions of a-amino acid ester hydrochlorides with 2, 4, 6-triphenylpyrylium tetrafluoroborate. Protonation, addition and substitution reactions of 4 with electrophiles were studied in this paper.
基金support from National Natural Science Foundation of China(No.21504046)Natural Science Foundation of Jiangsu Province(No.BK20150970)+1 种基金the Six Talent Peaks Project in Jiangsu Province(SWYY-060)the Projects of Nanjing Normal University(No.184080H20192,184080H10386).
文摘Good’s buffers have been widely applied in cell/organ culture over the past half a century as biocompatible pH stabilizers.However,the emergence of severe adverse effects,such as cellular uptake,lysosomal autophagic activation,and visible light-induced cytotoxicity,raises serious questions over its biocompatibility while underlying mechanism was unclear.Here we report that riboflavin(RF,component of cell culture medium)generates ^(1)O_(2),⋅OH,and O_(2)^(·-)under visible light exposure during regular cell manipulation.These short half-life reactive oxygen species(ROS)react with tertiary amine groups of HEPES,producing 106.6μM of H_(2)O_(2).Orders of magnitude elevated half-life of ROS in the medium caused severe cytotoxicity and systematic disorder of normal cell functions.We have further designed and validated zwitterionic betaines as the new generation biocompatible organic pH buffers,which is able to completely avoid the adverse effects that found on HEPES and derivate Good’s buffers.These findings may also open a new avenue for zwitterionic betaine based materials for biomedical applications.
基金supported by the Natural Science Foundation of Shanghai(No.24ZR1419600).
文摘Plant-related organic compound(PROC)may interact with redox-active metals like iron while they are present in soil or aquatic environment,but their effects on the photoreduction of Fe(III)remain largely unexplored.This study investigates the photochemical behavior of Fe(III)-PROC complexes using alkaline lignin(AL),betaine hydrochloride(BH),and phytic acid(PA)as representative proxies for PROC.The reductive agent AL demonstrated the ability to directly reduce Fe(III)to Fe(II).In contrast,BH,being unable to form strong complexes with Fe(III),was able to quench·OH,thereby resulting in a shift of the redox equilibrium towards Fe(II).PA exhibited a strong binding affinity for Fe(III),effectively inhibiting its photoreduction.Electron paramagnetic resonance(EPR)analysis,utilizing 5,5-dimethyl-1-pyrroline-N-oxide(DMPO)as a spin trap,revealed that the DMPO-OH signal detected in photolyzed Fe(III)-PROC solutions originated from various pathways.Specifically,uncomplexed Fe(III)in AL or BH solutions was shown to oxidize DMPO directly,leading to the formation of a false DMPO-OH adduct.The addition of ethanol to the photolyzed Fe(III)-AL and Fe(III)-BH systems resulted in the generation of the DMPO-CH(CH_(3))OH adduct,thereby confirming the presence of authentic·OH in these systems.The photolysis of the Fe(III)-PA complex may proceed via a photodissociation mechanism,where the resulting loosely bound Fe(III)can oxidize DMPO,followed by a nucleophilic attack from water.This research highlights the multifaceted roles of PROC in facilitating the redox cycling of iron within soil and aquatic ecosystems.
基金funded by the National Natural Science Foundation of China(grant numbers 32371244 and 92057118 to M.H.)and the 2023 Shanghai Eastern Talent Plan Leading Project(to M.H.)supported by the innovative research team of high-level local universities in Shanghai(grant numbers SHSMUZDCX20212000 and SHSMU-ZDCX20211202 to M.H.)+1 种基金the Shanghai Frontiers Science Center of Cellular Homeostasis and Human Diseases,and the Fundamental Research Funds for the Central Universities to M.H.labsupported by the Natural Science Foundation of Guangxi Zhuang Autonomous Region(grant number 2025GXNSFAA069105 to M.H.).
文摘Exercise,as a non-pharmacological health intervention,has been widely recognized for its beneficial effects,yet its underlying molecular mechanisms remain incompletely understood.The duration,frequency,and intensity of exercise exert distinct physiological impacts on the human body[1].Notably,acute exercise(AE)primarily elicits immediate metabolic responses and immune activation to cope with environmental stimuli,whereas long-term exercise(LE)induces cumulative health benefits across multiple organ systems[2‒4].Aging represents a complex biological process that persists throughout the ontogenetic continuum and serves as a pivotal etiological determinant for numerous chronic pathologies.In the context of accelerating global demographic aging,the development of interventions to promote healthspan extension and modulate aging trajectories has become a paramount research imperative in geroscience.Currently,research on the relationship between exercise and aging is a hot topic.For example,exercise has been shown to modulate aging through pathways such as AMP-activated protein kinase(AMPK)[5].However,the precise molecular links remain elusive.In a recent breakthrough study,Geng et al.used a novel multi-omics strategy to pinpoint betaine,a glycine derivative from choline/diet that serves as both a hepatic methyl donor and a renal osmoprotectant,as a key exercise-induced molecule with anti-inflammatory and geroprotective effects mediated partially via TANK-binding kinase 1(TBK1)inhibition[6,7].This work represents a significant advance as it systematically maps the molecular divergence between acute and long-term exercise while establishing a direct link between renal metabolism and systemic senescence-delaying benefits.
基金Supported by Guangxi Science and Technology Major Projects,No.2023AA20009National Natural Science Foundation of China,No.32360035 and No.32060018.
文摘BACKGROUND Skin wounds are highly common in diabetic patients,and with increasing types of pathogenic bacteria and antibiotic resistance,wounds and infections in diabetic patients are difficult to treat and heal.AIM To explore the effects of betaine ointment(BO)in promoting the healing of skin wounds and reducing the inflammation and apoptosis of skin cells in microbially infected diabetic mice.METHODS By detecting the minimum inhibitory concentrations(MICs)of betaine and plant monomer components such as psoralen,we prepared BO with betaine as the main ingredient,blended it with traditional Chinese medicines such as gromwell root and psoralen,and evaluated its antibacterial effects and safety in vitro and in vivo.The skin infection wound models of ordinary mice and diabetic mice were constructed,and the OTC drugs mupirocin ointment and Zicao ointment were used as controls to evaluate the antibacterial effects in vivo and the anti-inflammatory and anti-apoptotic effects of BO.RESULTS The MICs of betaine against microorganisms such as Staphylococcus aureus(S.aureus),Candida albicans and Cryptococcus neoformans ranged from 4 to 32μg/mL.Gromwell root and psoralea,both of which contain antimicrobial components,mixed to prepare BO with MICs ranging from 16 to 64μg/mL,which is 32-256 times lower than those of Zicao ointment,although the MIC is greater than that of betaine.After 15 days of treatment with BO for USA300-infected ordinary mice,the wound scab removal rates were 83.3%,while those of mupirocin ointment and Zicao ointment were 66.7%and 0%,respectively,and the differences were statistically significant.In diabetic mice,the wound scab removal rate of BO and mupirolacin ointment was 80.0%,but BO reduced wound inflammation and the apoptosis of skin cells and facilitated wound healing.CONCLUSION The ointment prepared by mixing betaine and traditional Chinese medicine can effectively inhibit common skin microorganisms and has a strong effect on the skin wounds of sensitive or drug-resistant S.aureus-infected ordinary mice and diabetic mice.
基金Projects(51274108,21263007,51204080)supported by the National Natural Science Foundation of ChinaProject(2011FA009)supported by the Applied Research Foundation of Yunnan Province,ChinaProject(14118441)supported by the Talents Cultivation Foundation of Kunming University of Science and Technology,China
文摘The electrodeposition behaviors of nickel on glassy carbon（GC） and carbon steel（CS） electrodes were investigated in the14.3%-85.7%（mole fraction） betaine.HCl ethylene glycol（EG） ionic liquid using cyclic voltammetry and chronoamperometry.The results indicated that the reduction of Ni（Ⅱ） on CS electrode via a diffusion-controlled quasi-reversible process was much more facile and easier than that occurred on GC electrode,which followed a diffusion-controlled three-dimensional instantaneous nucleation and growth.Scanning electron microscopy was used to observe that the deposit was dense and contained fine crystallites with average size of（80±4） nm.Energy dispersive spectrometer analysis showed that the obtained deposit was metallic nickel.X-ray diffraction spectroscopy indicated that（111） plane was the most preferred crystal orientation.The nickel deposit was luminous and bright,and had good adhesion with the CS substrate.
基金Supported by the National Natural Science Foundation of China(30660015)Key Laboratory of Vegetable Genetics and Physiology of Ministry of Agriculture of the People s Republic of China(2003-2005)~~
文摘[Objective] The research aimed to discuss the relationship between Glycine betaine and the salt-tolerance mechanism of different pumpkins and confirm the mechanism of betaine in salt-tolerance physiology.[Method] Taking the seedlings of C.ficifolia and Qingli pumpkin as test materials,the effects of glycine betaine on the cell membrane permeability,MDA and root activity of two varieties of pumpkin seedlings under 300 mmol/L NaCl stress were studied.[Result] At suitable concentrations,glycine betaine could d...
文摘以盐酸甜菜碱(Betaine·HCl)、乙二醇(EG)和六水合硫酸镍(NiSO_4·6H_2O)制备的Betaine·HCl-6EG-nNiSO_4·6H_2O低共熔离子液体为电解液,在碳钢(CS)基体上电沉积出平整致密的金属镍镀层.采用旋转黏度计和电导率仪分别测定了其黏度和电导率.在测试条件下,其黏度为3.918~55.19 m Pa·s,电导率为1.109~0.199 S/m.循环伏安测试结果表明Betaine·HCl-6EG-nNiSO_4·6H_2O(n=0.071)低共熔离子液体中Ni^(2+)在CS电极上的电化学还原过程可以描述为Ni^(2+)→Ni^0,其起始还原电位为-0.56 V.对CS基体上的沉积层进行XRD和EDAS测试,分析结果表明镀层为具有面心立方晶型结构的金属镍.SEM结果表明得到的镍镀层晶粒细小致密且均匀.镀层截面组织金相照片和线性扫描能谱图表明沿CS基体各处均得到厚度分布均匀且与基体结合良好的镍镀层,其厚度约为11μm.
文摘[Objective] This study aimed to analyze the effects of different concentrations of glycine betaine（GB） on oxidation metabolism in cucumbers under low-temperature stress and to investigate the possible mechanism of low-temperature resistance in cucumber during low-temperature storage. [Method] Cucumber cultivar Zhongnong No.8 was treated with 0, 5, 10 and 15 mmol/L GB solutions for 15 min and stored at 4 ℃. Changes in oxidative metabolism-related parameters were observed. [Result] Increasing exogenous GB concentration could enhance GB content in cucumbers, decline lipoxygenase（LOX） activity, improve peroxidase（POD） and catalase（CAT） activities, remove effectively hydrogen peroxide（H2O2） and reduce the accumulation of malondialdehyde（MDA）. [Conclusion] Treating cucumbers with10 mmol/L GB exhibited the most remarkable effect.
基金the Natural Science Foundation of Guangdong province (No. 970154).
文摘1, 3 Dipolar cycloaddition reaction between vinyl acetate and 3-hydroxypyridiniun betaine was performed under solid-liquid phase transfer catalytic condition, This reaction has been successfully used on the synthesis of an analogue of Bao-Gong-Teng A.
文摘AIM:To test whether ethanol feeding could induce Toll-like receptor 4(TLR4)responses,assess the hepatoprotective effect of betaine and its inhibitive effect on TLR4 in animal models of alcoholic liver injury.METHODS:Forty-eight female Sprague-Dawley rats were randomly divided into four groups as control,model,low and high dose betaine groups.Except control group,all rats were fed with high fat-containing diet plus ethanol and fish oil gavages for 8 wk.Betaine was administered intragastrically after exposure of ethanol for 4 wk.The changes of liver histology were examined.The expression of TLR4 mRNA and protein was detected by RT-PCR and Western blotting,respectively.The serum aminotransferase activity alanine transarninase(ALT),aspartate aminotransferase(AST),serum endotoxin,and liver inflammatory factors tumor necrosis factor-α(TNF-α),interferon-γ(IFN-γ),interleukin-18(IL-18)were also assayed.RESULTS:Compared with control group,rats of model group developed marked liver injury,accompanied by an increase of ALT(159.41±7.74 U/L vs 59.47± 2.34 U/L,P<0.0001),AST(248.25±1.40 U/L vs 116.89±3.48 U/L,P<0.0001),endotoxin(135.37± 30.17 ng/L vs 44.15±7.54 ng/L,P<0.0001),TNF-α(20.81±8.58 pg/mL vs 9.34±2.57 pg/mL,P=0.0003),IFN-γ(30.18±7.60 pg/mL vs 16.86±9.49 pg/mL,P= 0.0039)and IL-18(40.99±8.25 pg/mL vs 19.73±9.31 pg/mL,P=0.0001).At the same time,the expression of TLR4 mRNA and protein was markedly induced in the liver after chronic ethanol consumption(1.45±0.07 vs 0.44±0.04,P<0.0001;1.83±0.13 vs 0.56±0.08,P<0.0001).Compared with model group,betaine feeding resulted in significant decreases of ALT(64.93 ±6.06 U/L vs 159.41±7.74 U/L,P<0.0001),AST(188.73±1.11 U/L vs 248.25±1.40 U/L,P<0.0001),endotoxin(61.80±12.56 ng/L vs 135.37±30.17 ng/L,P<0.0001),TNF-α(9.79±1.32 pg/mL vs 20.81± 8.58 pg/mL,P=0.0003),IFN-γ(18.02±5.96 pg/mL vs 30.18±7.60 pg/mL,P=0.0008)and IL-18(18.23±7.01 pg/mL vs 40.99±8.25 pg/mL,P<0.0001).Betaine also improved liver steatosis.The expression levels of TLR4 mRNA or protein in liver tissues were significantly lowered(0.62±0.04 vs 1.45±0.07,P<0.0001;and 0.65±0.06 vs 1.83±0.13,P<0.0001).There was a statistical difference of TLR4 mRNA and protein expression between high-and low-dose betaine groups(0.62±0.04 vs 0.73±0.05,P<0.0001,and 0.65±0.06 vs 0.81±0.09,P<0.0001).CONCLUSION:Betaine can prevent the alcoholinduced liver injury effectively and improve the liver function.The expression of TLR4 increases significantly in ethanol-fed rats and betaine administration can inhibit TLR4 expression.
文摘Plants synthesize the osmoprotectant glycine betaine (GB) via choline→betaine aldehyde→glycine be- taine[1]. Two enzymes are involved in the pathway choline monooxygenase (CMO) and betaine aldehyde dehydrogenase (BADH). A full length CMO cDNA (1,643bp) was cloned from Amaranthus tricolor. The open reading frame encoded a 442-amino acid polypeptide, which showed 69% identity with CMOs in Spina- cia oleracea L. and Beta vulgaris L. DNA gel blot analysis indicated the presence of one copy of CMO gene in the A. tricolor genome. The expressions of CMO and BADH proteins in A.tricolor leaves significantly increased under salinization, drought and heat stress (42℃), as determined by immunoblot analysis, but did not respond to cold stress (4℃), or exogenous ABA application. The increase of GB content in leaves was parallel to CMO and BADH contents.
基金supported by the National Center for Genetic Engineering and Biotechnology (BIOTEC) (Grant No. BT-B-06-RG-14-4502)partly funded by the International Atomic Energy Agency (IAEA) (Contract No. 12998/R0)
文摘Glycinebetaine （Glybet） accumulation, photosynthetic efficiency and growth performance in indica rice cultivated under salt stress and extreme pH stress were investigated. Betaine aldehyde dehydrogenase （BADH） activity and Glybet accumulation in the seedlings of salt-tolerant and salt-sensitive rice varieties grown under saline and acidic conditions peaked after treatment for 72 h and 96 h, respectively, and were higher than those grown under neutral pH and alkaline salt stress. A positive correlation was found between BADH activity and Glybet content in both salt-tolerant （P=0.71） and salt-sensitive （P=0.86） genotypes. The chlorophyll a, chlorophyll b, total chlorophyll and total carotenoids contents in the stressed seedlings significantly decreased under both acidic and alkaline stresses, especially in the salt-sensitive genotype. Similarly, the maximum quantum yield of PSII （Fv/Fm）, photon yield of PSII （ФPSII）, non-photochemical quenching （NPQ） and net photosynthetic rate （Pn） in the stressed seedlings were inhibited, leading to overall growth reduction. The positive correlations between chlorophyll a content and Fv/Fm, total chlorophyll content and ФPSII, and Pn as well as Pn and leaf area in both salt-tolerant and salt-sensitive genotypes were found. Saline acidic and saline alkaline soils may play a key role affecting vegetative growth prior to the reproductive stage in rice plants.
基金funded and supported by National Basic Research Program of China(No.2012CB124705)Zhejiang Provincial Key Research and Development Program(2015C03006)Provincial Key S&T Special Projects(2015C02022)
文摘Background： Betaine affects fat metabolism in animals, but the specific mechanism is still not clear. The purpose of this study was to investigate possible mechanisms of betaine in altering lipid metabolism in muscle tissue in finishing pigs.Methods： A total of 120 crossbred gilts（Landrace × Yorkshire × Duroc） with an average initial body weight of 70.1 kg were randomly allotted to three dietary treatments. The treatments included a corn–soybean meal basal diet supplemented with 0, 1250 or 2500 mg/kg betaine. The feeding experiment lasted 42 d.Results： Betaine addition to the diet significantly increased the concentration of free fatty acids（FFA） in muscle（P 〈 0.05）. Furthermore, the levels of serum cholesterol and high-density lipoprotein cholesterol were decreased（P 〈 0.05） and total cholesterol content was increased in muscle（P 〈 0.05） of betaine fed pigs. Experiments on genes involved in fatty acid transport showed that betaine increased expression of lipoprotein lipase（LPL）, fatty acid translocase/cluster of differentiation（FAT/CD36）, fatty acid binding protein（FABP3） and fatty acid transport protein（FATP1）（P 〈 0.05）. The abundance of fatty acid transport protein and fatty acid binding protein were also increased by betaine（P 〈 0.05）. As for the key factors involved in fatty acid oxidation, although betaine supplementation didn＇t affect the level of carnitine and malonyl-CoA, betaine increased mR NA and protein abundance of carnitine palmitransferase-1（CPT1）and phosphorylated-AMPK（P 〈 0.05）.Conclusions： The results suggested that betaine may promoted muscle fatty acid uptake via up-regulating the genes related to fatty acid transporter including FAT/CD36, FATP1 and FABP3. On the other hand, betaine activated AMPK and up-regulated genes related to fatty acid oxidation including PPARα and CPT1. The underlying mechanism regulating fatty acid metabolism in pigs supplemented with betaine is associated with the up-regulation of genes involved in fatty acid transport and fatty acid oxidation.
文摘Nonalcoholic steatohepatitis (NASH) is an important indication for liver transplantation in many Western countries. Obesity and insulin resistance are the two most common risk factors for NASH, which can lead to recurrent NASH after liver transplantation. There is currently no approved therapy for NASH, and treatment is directed at risk factor modification and lifestyle changes. Betaine has been used for NASH, with mixed results, and may show promise in conjunction with other agents in clinical trials.
