Fruit cracking is a common phenomenon during the growth and development of horticultural crops that seriously affects fruit yield and quality.However,there are few studies on the mining of candidate genes related to b...Fruit cracking is a common phenomenon during the growth and development of horticultural crops that seriously affects fruit yield and quality.However,there are few studies on the mining of candidate genes related to berry cracking.To better understand the genetic basis of grape berry cracking,we conducted a genome-wide association study(GWAS)of grape varieties.Based on the mixed linear model(MLM),we detected five single nucleotide polymorphism(SNP)loci associated with berry-cracking index and two SNP loci associated with berry-cracking type in two years.These loci were mainly distributed on four chromosomes,namely 1,2,3,and 18,and were associated with ten unique candidate berry-cracking genes.The gene expression patterns indicated that the candidate genes in the susceptible berrycracking variety were more abundant than in the resistant berry-cracking variety.Grape berry-cracking is a complex trait controlled by multiple genes,mainly including genes involved in polygalacturonase,copper transporter,and receptor-like proteins.The high expression of the candidate berry-cracking genes may promote the occurrence of berry cracking,so the present study helps to further elucidate the genetic mechanism of berry cracking.展开更多
Fruit cracking is a phenomenon in which the peel cracks during grape berry development,which seriously affects the yield and quality of the fruit.However,there are few studies on the mining of candidate genes related ...Fruit cracking is a phenomenon in which the peel cracks during grape berry development,which seriously affects the yield and quality of the fruit.However,there are few studies on the mining of candidate genes related to berry cracking.In order to better understand the genetic basis of berry cracking,we used the results of previous quantitative trait locus(QTL)mapping,combined with field surveys of berry-cracking types and the berry-cracking rate,to mine candidate berry-cracking genes.The results showed that three identical QTL loci were detected in two years(2019 and 2020);and three candidate genes were annotated in the QTL interval.In mature berries,the expressions of the candidate genes were more abundant in the cracking-susceptible parent(‘Crimson Seedless’)than in the cracking-resistant parent(‘Muscat Hamburg’).Grape berry cracking is a complex trait controlled by multiple genes,mainly including genes encoding cellulose synthase–like protein H1,glucan endo-1,3-beta-glucosidase 12,and brassinosteroid insensitive 1-associated receptor kinase 1.The high expression of the candidate berry-cracking genes may promote the occurrence of berry cracking.This study helps elucidate the genetic mechanism of grape berry cracking.展开更多
基金the National Key Research and Development Program of China(2019YFD1000101)the China Agriculture Research System of MOF and MARA(CARS-29-yc-1)Crop Resources Protection Program of Ministry of Agriculture and Rural Affairs of China(2130135–34)。
文摘Fruit cracking is a common phenomenon during the growth and development of horticultural crops that seriously affects fruit yield and quality.However,there are few studies on the mining of candidate genes related to berry cracking.To better understand the genetic basis of grape berry cracking,we conducted a genome-wide association study(GWAS)of grape varieties.Based on the mixed linear model(MLM),we detected five single nucleotide polymorphism(SNP)loci associated with berry-cracking index and two SNP loci associated with berry-cracking type in two years.These loci were mainly distributed on four chromosomes,namely 1,2,3,and 18,and were associated with ten unique candidate berry-cracking genes.The gene expression patterns indicated that the candidate genes in the susceptible berrycracking variety were more abundant than in the resistant berry-cracking variety.Grape berry-cracking is a complex trait controlled by multiple genes,mainly including genes involved in polygalacturonase,copper transporter,and receptor-like proteins.The high expression of the candidate berry-cracking genes may promote the occurrence of berry cracking,so the present study helps to further elucidate the genetic mechanism of berry cracking.
基金financial support from the Highlevel Scientific Reuter Foundation of Qingdao Agricultural University(Grant Nos.665/1118011,665/1119002)China Agriculture Research System of MOF and MARA(Grant No.CARS-29-yc-1)Crop Resources Protection Program of Ministry of Agriculture and Rural Affairs of China(Grant No.2130135-34).
文摘Fruit cracking is a phenomenon in which the peel cracks during grape berry development,which seriously affects the yield and quality of the fruit.However,there are few studies on the mining of candidate genes related to berry cracking.In order to better understand the genetic basis of berry cracking,we used the results of previous quantitative trait locus(QTL)mapping,combined with field surveys of berry-cracking types and the berry-cracking rate,to mine candidate berry-cracking genes.The results showed that three identical QTL loci were detected in two years(2019 and 2020);and three candidate genes were annotated in the QTL interval.In mature berries,the expressions of the candidate genes were more abundant in the cracking-susceptible parent(‘Crimson Seedless’)than in the cracking-resistant parent(‘Muscat Hamburg’).Grape berry cracking is a complex trait controlled by multiple genes,mainly including genes encoding cellulose synthase–like protein H1,glucan endo-1,3-beta-glucosidase 12,and brassinosteroid insensitive 1-associated receptor kinase 1.The high expression of the candidate berry-cracking genes may promote the occurrence of berry cracking.This study helps elucidate the genetic mechanism of grape berry cracking.
