Several probes containing benzothiazole-guided conjugated systems(BGCS) were designed and synthesized, and two molecules(BGCS5 and BGCS6) of which were discovered as selective probes targeting c-MYC Pu22 G-quadruplex ...Several probes containing benzothiazole-guided conjugated systems(BGCS) were designed and synthesized, and two molecules(BGCS5 and BGCS6) of which were discovered as selective probes targeting c-MYC Pu22 G-quadruplex DNA. The fluorescence intensity of BGCS5 and BGCS6 in the presence of c-MYC Pu22 far exceeds that of the typical G4 probe TO1. Especially, the fluorescence of BGCS6 increased almost193-fold in the presence of c-MYC Pu22 G4 compared to that alone in aqueous buffer condition with almost no fluorescence and 10–30 folds than those in the presence of other DNAs, which will be useful tools for disease detection in mammals.展开更多
基金financially supported by the Innovation and Application of Key Technologies of Quality-improving and Efficiency-increasing of Fengtou Ginger Industry (No. 2020–620–002–06)Natural Science Foundation of Hubei Province (No. 2020CFB717)+2 种基金support from the Program for Leading Talents of Hubei Academy of Agricultural Sciences (No. L2018031)the Youth Science Foundation of Hubei Academy of Agricultural Sciences (No. 2021NKYJJ17)Hubei Agricultural Science Innovation centre (No. 2019–620–000–001–27)。
文摘Several probes containing benzothiazole-guided conjugated systems(BGCS) were designed and synthesized, and two molecules(BGCS5 and BGCS6) of which were discovered as selective probes targeting c-MYC Pu22 G-quadruplex DNA. The fluorescence intensity of BGCS5 and BGCS6 in the presence of c-MYC Pu22 far exceeds that of the typical G4 probe TO1. Especially, the fluorescence of BGCS6 increased almost193-fold in the presence of c-MYC Pu22 G4 compared to that alone in aqueous buffer condition with almost no fluorescence and 10–30 folds than those in the presence of other DNAs, which will be useful tools for disease detection in mammals.
