The monofloral honey derived from Bauhinia championii(Benth.)Benth.(MH-Bc)possesses significant nutritional and bioactive value,making it highly suitable for commercial exploitation.However,the poorly defined characte...The monofloral honey derived from Bauhinia championii(Benth.)Benth.(MH-Bc)possesses significant nutritional and bioactive value,making it highly suitable for commercial exploitation.However,the poorly defined characteristics and unknown composition have hindered MH-Bc product development.In this study,we employed a combination of untargeted and targeted mass spectrometry analyses to characterize MH-Bc honey.As a result,4,7,8-trimethoxydibenzo[b,d]furan-3-ol(TDBF)was identified as a robust chemical marker for distinguishing MH-Bc from other types of honey.This specific marker was detected in both MH-Bc and the Bc plant but was absent in other honey varieties.Furthermore,a targeted mass spectrometry quantitative method was developed and validated to accurately determine the content of TDBF in honey samples.Overall,the presence of TDBF serves as a discerning indicator for future commercial MH-Bc products.展开更多
Crotalaria ferruginea Graham ex Benth.is a commonly used herb among ethnic minorities.Its whole plant is used as medicine for conditions such as heatstroke,tinnitus,hearing loss,phlegm heat cough,gum swelling and pain...Crotalaria ferruginea Graham ex Benth.is a commonly used herb among ethnic minorities.Its whole plant is used as medicine for conditions such as heatstroke,tinnitus,hearing loss,phlegm heat cough,gum swelling and pain,lower back and knee pain,vaginal discharge and infantile malnutrition.Modern pharmaceutical research has found that Crotalaria ferruginea Graham ex Benth.mainly contains flavonoids,steroids,organic acids and terpenes,which have antibacterial,free radical scavenging,antiinflammatory and other effects.Quality control mainly focuses on characteristics,identification,content determination,etc.This article summarizes the recent research progress on the chemical composition,pharmacological effects,quality control and extraction process of Crotalaria ferruginea Graham ex Benth.on the basis of existing Crotalaria ferruginea Graham ex Benth.research reports.展开更多
[Objective] This study aimed to understand the characters of hard seeds of Amphicarpaea edgeworthii Benth. （Leguminosae） and explore the methods for break- ing dormancy. [Method] For both aerial and subterranean see...[Objective] This study aimed to understand the characters of hard seeds of Amphicarpaea edgeworthii Benth. （Leguminosae） and explore the methods for break- ing dormancy. [Method] For both aerial and subterranean seeds of A. edgeworthii, the morphological characteristics were observed and the appropriate temperature for germination was explored. For aerial seeds of A. edgeworthii, the characters of hard seeds were studied, and concentrated sulfuric acid treatment, hot water soak treat- ment and mechanical damage treatment were compared to explore the methods for breaking seed dormancy. [Result] The aerial seeds were oblate and averaged 3.38 mm in length, 3.02 mm in width, 1.88 mm in thickness, 15.32 g in thousand grain weight, with a hard seed rate after of up to 98% natural maturation. The subter- ranean seeds were approximately oblate with a maximum diameter of 15 mm and a hundred grain weight of （50.08-58.26 g）; among all the treatments for breaking hardseededness, cutting seed coat treatment and concentrated sulfuric acid treatment for 20 minutes were the most effective methods, whereas hot water soak treatment was the least effective method; constant temperature between 20 and 30 ℃ was optimum for the germination of aerial seeds, and alternative temperature of 30/20 ℃ was most appropriate for the germination of subterranean seeds. [Conclusion] Cutting seed coat treatment and concentrated sulfuric acid treatment for 20 minutes were the most effective methods to break the hardseededness of A. edgeworthii.展开更多
The volatile constituents of the essential oil of Hymenocrater longiflorus Benth. growing wild in Kurdistan-Iran were investigated using the GC and GC/MS techniques. Fifteen compounds, representing twenty (97.03%) of ...The volatile constituents of the essential oil of Hymenocrater longiflorus Benth. growing wild in Kurdistan-Iran were investigated using the GC and GC/MS techniques. Fifteen compounds, representing twenty (97.03%) of the total oil were identified. The main components were: α- Pinene (22.47%), β-Caryophyllene (18.05%), β- Eudesmol (14.92%), α-Copaene (9.84%), γ-Elemene (6.79%), δ-Cadine-ne (6.13%), (–)Bornyl acetate (5.61%), α-Amorphene (3.84%), α-Fen- chyl acetate (2.35%) and β-Pinene (2.07%). Some of the physicochemical properties like the logarithm of calculated Octanol-Water partitioning coefficients (log Kow), total biodegradation and (TBd in mol/h and gr./h), water solubility (Sw, mg.L-1 at 25oC) and median lethal concentration 50 (LC50) were calculated for the 15 com- ponents of Hymenocrater longiflorus Benth.展开更多
[Objectives]To determine active components of Lamiophlomis rotata(Benth.)Kudo.[Methods]A new method was developed for the quantitative determination of L.rotata by using Ultra-performance convergence chromatography.Th...[Objectives]To determine active components of Lamiophlomis rotata(Benth.)Kudo.[Methods]A new method was developed for the quantitative determination of L.rotata by using Ultra-performance convergence chromatography.The separation of active components of L.rotata was successfully achieved on Waters ACQUITY UPC column,with gradient elution carbon dioxide and methanol solvent at flow rate of 0.7 mL/min.The separation gradient elution rate was as follows:15%methanol 0-1 min,18%methanol 1-5 min,20%methanol 5-6 min,25%methanol 6-7 min,30%methanol 7-8 min,30%methanol 8-9 min,15%methanol 9-10 min.The conditions used in the separation process was as follows:column temperature 30℃;injection volume 2μL.Under the abovementioned conditions,the content of methyl glucoside was measured by 3D-full wavelength scanning.The validity of the method was tested by analyzing the precision,reproducibility,stability and accuracy.[Results]The results indicated that in terms of extraction and separation of compounds,supercritical CO2 not only has the diffusion property of gas,but also has the density and fluidity of liquid.[Conclusions]The ultra-high performance chromatography is a new technique for drug analysis.展开更多
[Objective] The variation of sugar, acid and AsA contents in fruits of ‘Ganmi 6＇ kiwifruit （Actinidia eriantha Benth） were investigated during fruit develop- ment. [Method] Kiwi fruits were randomly taken as mater...[Objective] The variation of sugar, acid and AsA contents in fruits of ‘Ganmi 6＇ kiwifruit （Actinidia eriantha Benth） were investigated during fruit develop- ment. [Method] Kiwi fruits were randomly taken as materials every 15 days since 20 days after full bloom （DAFB） to 170 DAFB until in mature stage. [Result] The results showed that during fruit development of ‘Ganmi 6＇, the total sugar soluble contents had a rising trend with relatively stable at 95 DAFB, then rose until har- vest with the maximum content （10.35%）. The titratable acid content showed a trend of increasing, then declining, then increase to the harvest content （1.10%）. From the sugar acid ratio, we can knew it decreased in 95 DAFB, then up to the max （9.38）. The changes of AsA contents showed double ‘S＇ shape, decreased af- ter the first increased rapidly, slightly increased and then decreased in the early harvest. [Conclusion] It provided a theoretical basis for scientific cultivation methods to explore the nutrients regulation.展开更多
基金supported by the Natural Science Foundation of Beijing Municipality(6252026)the Youth Innovation Program of CAAS(Y2024QC11)the Agricultural Science and Technology Innovation Program under Grant(CAAS-ASTIP-2024-IAR).
文摘The monofloral honey derived from Bauhinia championii(Benth.)Benth.(MH-Bc)possesses significant nutritional and bioactive value,making it highly suitable for commercial exploitation.However,the poorly defined characteristics and unknown composition have hindered MH-Bc product development.In this study,we employed a combination of untargeted and targeted mass spectrometry analyses to characterize MH-Bc honey.As a result,4,7,8-trimethoxydibenzo[b,d]furan-3-ol(TDBF)was identified as a robust chemical marker for distinguishing MH-Bc from other types of honey.This specific marker was detected in both MH-Bc and the Bc plant but was absent in other honey varieties.Furthermore,a targeted mass spectrometry quantitative method was developed and validated to accurately determine the content of TDBF in honey samples.Overall,the presence of TDBF serves as a discerning indicator for future commercial MH-Bc products.
文摘Crotalaria ferruginea Graham ex Benth.is a commonly used herb among ethnic minorities.Its whole plant is used as medicine for conditions such as heatstroke,tinnitus,hearing loss,phlegm heat cough,gum swelling and pain,lower back and knee pain,vaginal discharge and infantile malnutrition.Modern pharmaceutical research has found that Crotalaria ferruginea Graham ex Benth.mainly contains flavonoids,steroids,organic acids and terpenes,which have antibacterial,free radical scavenging,antiinflammatory and other effects.Quality control mainly focuses on characteristics,identification,content determination,etc.This article summarizes the recent research progress on the chemical composition,pharmacological effects,quality control and extraction process of Crotalaria ferruginea Graham ex Benth.on the basis of existing Crotalaria ferruginea Graham ex Benth.research reports.
基金Supported by the"Twelfth Five-Year"Key Project for Crop Breeding of Sichuan Province(YZGG4-6)the Special Support Program for the Scientific Research Personnel of Sichuan Agricultural University~~
文摘[Objective] This study aimed to understand the characters of hard seeds of Amphicarpaea edgeworthii Benth. （Leguminosae） and explore the methods for break- ing dormancy. [Method] For both aerial and subterranean seeds of A. edgeworthii, the morphological characteristics were observed and the appropriate temperature for germination was explored. For aerial seeds of A. edgeworthii, the characters of hard seeds were studied, and concentrated sulfuric acid treatment, hot water soak treat- ment and mechanical damage treatment were compared to explore the methods for breaking seed dormancy. [Result] The aerial seeds were oblate and averaged 3.38 mm in length, 3.02 mm in width, 1.88 mm in thickness, 15.32 g in thousand grain weight, with a hard seed rate after of up to 98% natural maturation. The subter- ranean seeds were approximately oblate with a maximum diameter of 15 mm and a hundred grain weight of （50.08-58.26 g）; among all the treatments for breaking hardseededness, cutting seed coat treatment and concentrated sulfuric acid treatment for 20 minutes were the most effective methods, whereas hot water soak treatment was the least effective method; constant temperature between 20 and 30 ℃ was optimum for the germination of aerial seeds, and alternative temperature of 30/20 ℃ was most appropriate for the germination of subterranean seeds. [Conclusion] Cutting seed coat treatment and concentrated sulfuric acid treatment for 20 minutes were the most effective methods to break the hardseededness of A. edgeworthii.
文摘The volatile constituents of the essential oil of Hymenocrater longiflorus Benth. growing wild in Kurdistan-Iran were investigated using the GC and GC/MS techniques. Fifteen compounds, representing twenty (97.03%) of the total oil were identified. The main components were: α- Pinene (22.47%), β-Caryophyllene (18.05%), β- Eudesmol (14.92%), α-Copaene (9.84%), γ-Elemene (6.79%), δ-Cadine-ne (6.13%), (–)Bornyl acetate (5.61%), α-Amorphene (3.84%), α-Fen- chyl acetate (2.35%) and β-Pinene (2.07%). Some of the physicochemical properties like the logarithm of calculated Octanol-Water partitioning coefficients (log Kow), total biodegradation and (TBd in mol/h and gr./h), water solubility (Sw, mg.L-1 at 25oC) and median lethal concentration 50 (LC50) were calculated for the 15 com- ponents of Hymenocrater longiflorus Benth.
基金Collaborative Innovation in Tibetan Medicine(2019XTC013)Open Laboratory of Key Laboratory of Tibetan Medicine Basic Education(ZYYJC-19-01).
文摘[Objectives]To determine active components of Lamiophlomis rotata(Benth.)Kudo.[Methods]A new method was developed for the quantitative determination of L.rotata by using Ultra-performance convergence chromatography.The separation of active components of L.rotata was successfully achieved on Waters ACQUITY UPC column,with gradient elution carbon dioxide and methanol solvent at flow rate of 0.7 mL/min.The separation gradient elution rate was as follows:15%methanol 0-1 min,18%methanol 1-5 min,20%methanol 5-6 min,25%methanol 6-7 min,30%methanol 7-8 min,30%methanol 8-9 min,15%methanol 9-10 min.The conditions used in the separation process was as follows:column temperature 30℃;injection volume 2μL.Under the abovementioned conditions,the content of methyl glucoside was measured by 3D-full wavelength scanning.The validity of the method was tested by analyzing the precision,reproducibility,stability and accuracy.[Results]The results indicated that in terms of extraction and separation of compounds,supercritical CO2 not only has the diffusion property of gas,but also has the density and fluidity of liquid.[Conclusions]The ultra-high performance chromatography is a new technique for drug analysis.
基金Supported by the Special Science Foundation of Jiangxi Province(20143ACF60015)National Natural Scientific Fund(31360472)~~
文摘[Objective] The variation of sugar, acid and AsA contents in fruits of ‘Ganmi 6＇ kiwifruit （Actinidia eriantha Benth） were investigated during fruit develop- ment. [Method] Kiwi fruits were randomly taken as materials every 15 days since 20 days after full bloom （DAFB） to 170 DAFB until in mature stage. [Result] The results showed that during fruit development of ‘Ganmi 6＇, the total sugar soluble contents had a rising trend with relatively stable at 95 DAFB, then rose until har- vest with the maximum content （10.35%）. The titratable acid content showed a trend of increasing, then declining, then increase to the harvest content （1.10%）. From the sugar acid ratio, we can knew it decreased in 95 DAFB, then up to the max （9.38）. The changes of AsA contents showed double ‘S＇ shape, decreased af- ter the first increased rapidly, slightly increased and then decreased in the early harvest. [Conclusion] It provided a theoretical basis for scientific cultivation methods to explore the nutrients regulation.
