Crotalaria ferruginea Graham ex Benth.is a commonly used herb among ethnic minorities.Its whole plant is used as medicine for conditions such as heatstroke,tinnitus,hearing loss,phlegm heat cough,gum swelling and pain...Crotalaria ferruginea Graham ex Benth.is a commonly used herb among ethnic minorities.Its whole plant is used as medicine for conditions such as heatstroke,tinnitus,hearing loss,phlegm heat cough,gum swelling and pain,lower back and knee pain,vaginal discharge and infantile malnutrition.Modern pharmaceutical research has found that Crotalaria ferruginea Graham ex Benth.mainly contains flavonoids,steroids,organic acids and terpenes,which have antibacterial,free radical scavenging,antiinflammatory and other effects.Quality control mainly focuses on characteristics,identification,content determination,etc.This article summarizes the recent research progress on the chemical composition,pharmacological effects,quality control and extraction process of Crotalaria ferruginea Graham ex Benth.on the basis of existing Crotalaria ferruginea Graham ex Benth.research reports.展开更多
[Objective] The variation of sugar, acid and AsA contents in fruits of ‘Ganmi 6＇ kiwifruit （Actinidia eriantha Benth） were investigated during fruit develop- ment. [Method] Kiwi fruits were randomly taken as mater...[Objective] The variation of sugar, acid and AsA contents in fruits of ‘Ganmi 6＇ kiwifruit （Actinidia eriantha Benth） were investigated during fruit develop- ment. [Method] Kiwi fruits were randomly taken as materials every 15 days since 20 days after full bloom （DAFB） to 170 DAFB until in mature stage. [Result] The results showed that during fruit development of ‘Ganmi 6＇, the total sugar soluble contents had a rising trend with relatively stable at 95 DAFB, then rose until har- vest with the maximum content （10.35%）. The titratable acid content showed a trend of increasing, then declining, then increase to the harvest content （1.10%）. From the sugar acid ratio, we can knew it decreased in 95 DAFB, then up to the max （9.38）. The changes of AsA contents showed double ‘S＇ shape, decreased af- ter the first increased rapidly, slightly increased and then decreased in the early harvest. [Conclusion] It provided a theoretical basis for scientific cultivation methods to explore the nutrients regulation.展开更多
[Objective] This study aimed to understand the characters of hard seeds of Amphicarpaea edgeworthii Benth. （Leguminosae） and explore the methods for break- ing dormancy. [Method] For both aerial and subterranean see...[Objective] This study aimed to understand the characters of hard seeds of Amphicarpaea edgeworthii Benth. （Leguminosae） and explore the methods for break- ing dormancy. [Method] For both aerial and subterranean seeds of A. edgeworthii, the morphological characteristics were observed and the appropriate temperature for germination was explored. For aerial seeds of A. edgeworthii, the characters of hard seeds were studied, and concentrated sulfuric acid treatment, hot water soak treat- ment and mechanical damage treatment were compared to explore the methods for breaking seed dormancy. [Result] The aerial seeds were oblate and averaged 3.38 mm in length, 3.02 mm in width, 1.88 mm in thickness, 15.32 g in thousand grain weight, with a hard seed rate after of up to 98% natural maturation. The subter- ranean seeds were approximately oblate with a maximum diameter of 15 mm and a hundred grain weight of （50.08-58.26 g）; among all the treatments for breaking hardseededness, cutting seed coat treatment and concentrated sulfuric acid treatment for 20 minutes were the most effective methods, whereas hot water soak treatment was the least effective method; constant temperature between 20 and 30 ℃ was optimum for the germination of aerial seeds, and alternative temperature of 30/20 ℃ was most appropriate for the germination of subterranean seeds. [Conclusion] Cutting seed coat treatment and concentrated sulfuric acid treatment for 20 minutes were the most effective methods to break the hardseededness of A. edgeworthii.展开更多
To explore the optimum temperature,light intensityand water conditions for seed germination of Siphonostegia chinensis Benth.,seed germination experiment were carried out under different temperatures(5/15,10/20,15/25,...To explore the optimum temperature,light intensityand water conditions for seed germination of Siphonostegia chinensis Benth.,seed germination experiment were carried out under different temperatures(5/15,10/20,15/25,20/30℃),different light intensity(14h light/10h darkness,complete darkness)and different concentrations(0%,5%,10%,15%,20%)of PEG-6000 solution.In terms of concentration,5%PEG was regarded as the low level,10%and 15%as the medium level,and 20%as the high level.The results showed that(1)Germination rate,germination potential,and germination index were increased with the rise of temperature.In addition,seed germination was significantly higher under the dark conditions than that with the 14h light/10h darkness.(2)No seed germination occurred when the temperature was below 10/20 C at 14h light/10h darkness.(3)Under 14h light/10h darkness,the germination rate,germination potential and germination index first increased and then decreased with the increase of PEG concentration.The low concentration was more beneficial to the seed germination.(4)Under the condition of complete darkness,the germination rate,germination potential and germination index decline with fluctuation with the increase of PEG concentration.Seed germination of Siphonostegia chinensis Benth.was inhibited in high concentration of PEG.展开更多
Objective To establish an RP-HPLC method for the determination of β-Sitosterol in Elaeagnus Gonyanthes Benth.Methods The separation was performed on a luna C8(2)(150 mm×4.6 mm,5μm)column with the mobile phase o...Objective To establish an RP-HPLC method for the determination of β-Sitosterol in Elaeagnus Gonyanthes Benth.Methods The separation was performed on a luna C8(2)(150 mm×4.6 mm,5μm)column with the mobile phase of methanol-water(88∶12,v/v)at a flow rate of 1.0 mL/min,the detection wavelength was set at 210 nm,and the temperature of the column was maintained at 35 ℃.Results The calibration curve of β-Sitosterol was linear over the concentration range of 0.075-0.375 mg/mL(r=0.9999)and the average recovery of β-Sitosterol was 96.30% with RSD of 3.60%(n=3).Conclusion The method is simple,rapid,and accurate,and can be used for the quality control of Elaeagnus Gonyanthes Benth.展开更多
The cultivation technology of selenium-containing plant Pueraria thomsonii Benth was summarized from the producing area conditions,variety selection,field management and other aspects,which is of great significance fo...The cultivation technology of selenium-containing plant Pueraria thomsonii Benth was summarized from the producing area conditions,variety selection,field management and other aspects,which is of great significance for increasing the selenium concentration in humans or animals.展开更多
[Objectives]To determine active components of Lamiophlomis rotata(Benth.)Kudo.[Methods]A new method was developed for the quantitative determination of L.rotata by using Ultra-performance convergence chromatography.Th...[Objectives]To determine active components of Lamiophlomis rotata(Benth.)Kudo.[Methods]A new method was developed for the quantitative determination of L.rotata by using Ultra-performance convergence chromatography.The separation of active components of L.rotata was successfully achieved on Waters ACQUITY UPC column,with gradient elution carbon dioxide and methanol solvent at flow rate of 0.7 mL/min.The separation gradient elution rate was as follows:15%methanol 0-1 min,18%methanol 1-5 min,20%methanol 5-6 min,25%methanol 6-7 min,30%methanol 7-8 min,30%methanol 8-9 min,15%methanol 9-10 min.The conditions used in the separation process was as follows:column temperature 30℃;injection volume 2μL.Under the abovementioned conditions,the content of methyl glucoside was measured by 3D-full wavelength scanning.The validity of the method was tested by analyzing the precision,reproducibility,stability and accuracy.[Results]The results indicated that in terms of extraction and separation of compounds,supercritical CO2 not only has the diffusion property of gas,but also has the density and fluidity of liquid.[Conclusions]The ultra-high performance chromatography is a new technique for drug analysis.展开更多
The volatile constituents of the essential oil of Hymenocrater longiflorus Benth. growing wild in Kurdistan-Iran were investigated using the GC and GC/MS techniques. Fifteen compounds, representing twenty (97.03%) of ...The volatile constituents of the essential oil of Hymenocrater longiflorus Benth. growing wild in Kurdistan-Iran were investigated using the GC and GC/MS techniques. Fifteen compounds, representing twenty (97.03%) of the total oil were identified. The main components were: α- Pinene (22.47%), β-Caryophyllene (18.05%), β- Eudesmol (14.92%), α-Copaene (9.84%), γ-Elemene (6.79%), δ-Cadine-ne (6.13%), (–)Bornyl acetate (5.61%), α-Amorphene (3.84%), α-Fen- chyl acetate (2.35%) and β-Pinene (2.07%). Some of the physicochemical properties like the logarithm of calculated Octanol-Water partitioning coefficients (log Kow), total biodegradation and (TBd in mol/h and gr./h), water solubility (Sw, mg.L-1 at 25oC) and median lethal concentration 50 (LC50) were calculated for the 15 com- ponents of Hymenocrater longiflorus Benth.展开更多
[Objective] The study aims to optimize the extracting technology conditions of essential oil from Elsholtzia densa Benth. [Method] Essential oil was extracted from E. densa Benth using steam distillation, and the effe...[Objective] The study aims to optimize the extracting technology conditions of essential oil from Elsholtzia densa Benth. [Method] Essential oil was extracted from E. densa Benth using steam distillation, and the effects of four extracting factors (grain size of the medicinal herb, ultrasonic time, soaking time and ratio of the material to liquid) on the yield of essential oil extracted from E. densa Benth were analyzed through the orthogonal test. [Result] The optimized extraction process of essential oil from E. densa Benth was as follows: grain size of the medicinal herb 50 mesh, ultrasonic time 12 min, being soaked in the water for 6 h, and ratio of the material to liquid 1∶16 g/ml. Under the conditions, the average extracting rate of the essential oil was 0.407%. [Conclusion] The optimized extraction process of essential oil from E. densa Benth was simple and practicable, and its extracting rate was higher.展开更多
The twigs and leaves of Pithecellobium clypearia(Jack.)Benth are used as common Chinese traditional herbal medicine to treat upper respiratory tract infection,acute gastroenteritis and bacterial dysentery.The study is...The twigs and leaves of Pithecellobium clypearia(Jack.)Benth are used as common Chinese traditional herbal medicine to treat upper respiratory tract infection,acute gastroenteritis and bacterial dysentery.The study isolated nine compounds from the EtOAc extract,all of which were obtained from Pithecellobium Mart.for the first time.Based on 1H-NMR and 13C-NMR data,the structures of these compounds,including two ladiosiplodins and two dihydroisocoumarins,were elucidated.展开更多
Objective:To evaluate the anti-hepatocellular carcinoma(HCC)activity of total alkaloids from Gelsemium elegans Benth.(TAG)in vivo and in vitro and to elucidate their potential mechanisms of action through transcriptom...Objective:To evaluate the anti-hepatocellular carcinoma(HCC)activity of total alkaloids from Gelsemium elegans Benth.(TAG)in vivo and in vitro and to elucidate their potential mechanisms of action through transcriptomic analysis.Methods:TAG extraction was conducted,and the primary components were quantified using high-performance liquid chromatography(HPLC).The effects of TAG(100,150,and 200μg/mL)on various tumor cells,including SMMC-7721,HepG2,H22,CAL27,MCF7,HT29,and HCT116,were assessed.Effects of TAG on HCC proliferation and apoptosis were detected by colony formation assays and cell stainings.Caspase-3,Bcl-2,and Bax protein levels were detected by Western blotting.In vivo,a tumor xenograft model was developed using H22 cells.Totally 40 Kunming mice were randomly assigned to model,cyclophosphamide(20 mg/kg),TAG low-dose(TAG-L,0.5 mg/kg),and TAG high-dose(TAG-H,1 mg/kg)groups,with 10 mice in each group.Tumor volume,body weight,and tumor weight were recorded and compared during 14-day treatment.Immune organ index were calculated.Tissue changes were oberseved by hematoxylin and eosin staining and immunohistochemistry.Additionally,transcriptomic and metabolomic analyses,as well as quatitative real-time polymerase chain reaction(RT-qPCR),were performed to detect mRNA and metabolite expressions.Results:HPLC successfully identified the components of TAG extraction.Live cell imaging and analysis,along with cell viability assays,demonstrated that TAG inhibited the proliferation of SMMC-7721,HepG2,H22,CAL27,MCF7,HT29,and HCT116 cells.Colony formation assays,Hoechst 33258 staining,Rhodamine 123 staining,and Western blotting revealed that TAG not only inhibited HCC proliferation but also promoted apoptosis(P<0.05).In vivo experiments showed that TAG inhibited the growth of solid tumors in HCC in mice(P<0.05).Transcriptomic analysis and RT-qPCR indicated that the inhibition of HCC by TAG was associated with the regulation of the key gene CXCL13.Conclusion:TAG inhibits HCC both in vivo and in vitro,with its inhibitory effect linked to the regulation of the keygene CXCL13.展开更多
Objective To explore the antitumor effects of ethanol extract from Ventilago leiocarpa Benth(EEVLB)on sarcoma 180(S180)tumor-bearing mice and the potential mechanism.Methods Sixty mice were randomly assigned to 6 grou...Objective To explore the antitumor effects of ethanol extract from Ventilago leiocarpa Benth(EEVLB)on sarcoma 180(S180)tumor-bearing mice and the potential mechanism.Methods Sixty mice were randomly assigned to 6 groups according to a random number table:normal group,model group,5-fluorouracil(5-FU)group(0.02 g·kg^(−1)),and high-,medium-,low-dose EEVLB groups(100,84,and 56 g of raw material·kg^(−1)body weight,respectively),with 10 mice each group.All treatments were given once daily for 10 consecutive days.Effects of EEVLB on inhibiting tumor growth and immune function in mice were evaluated among all groups after the treatments by detecting tumor inhibition rate,organ index,serum levels of interleukin(IL)-2,-6,-10,CD3^(+)CD4^(+)T lymphocytes,CD4^(+)/CD8^(+)ratio,caspase-3 and Bcl-2.Results EEVLB with different concentrations achieved inhibition of tumor growth in vivo,wherein the high-dose group showed the most significant reduction in tumor weight and increased apoptosis of tumor cells(P<0.05).In addition,both net weight gain and spleen index of mice showed uptrend in EEVLB treatment groups(P<0.05).Besides,serum levels of IL-2 and IL-6,percentages of CD3^(+)CD4^(+)T lymphocytes and ratio of CD4^(+)/CD8^(+)in peripheral blood were elevated in high-and medium-dose EEVLB groups compared with the model group(P<0.05).Also,upregulation of caspase-3 and downregulation of Bcl-2 were observed at protein levels in the high-dose EEVLB group(P<0.01).Conclusions EEVLB exhibits promising antitumor activity in vivo.This effect might be due to activation of apoptotic signaling pathway,increase of cytokine levels and enhancement of immune function in tumor-bearing mice.展开更多
Gelsemium elegans Benth alkaloids are the main components of G.elegans and can cause acute toxicosis or even death.Although several studies have reported methods for detecting G.elegans alkaloids,a high-throughput and...Gelsemium elegans Benth alkaloids are the main components of G.elegans and can cause acute toxicosis or even death.Although several studies have reported methods for detecting G.elegans alkaloids,a high-throughput and environmental-friendly strategy for detection of multiple G.elegans alkaloids has not been realized.In this work,a matrix-assisted laser desorption/ionization time-of-flight mass spectrometry method was developed for rapid detection of G.elegans alkaloids in human plasma and urine for diagnosis of poisoning.Multiple matrices and crys-tal spotting methods were evaluated to obtain stable and high peak intensities without“sweet spot”.We verified the methodology and obtained excellent results.The matrix effects with different dilutions were compared and good recoveries and a low relative standard deviation were obtained with a 40-fold dilution.This method could shorten the analysis time and greatly reduce the consumption of chemical solvents.Furthermore,it could be applied to quan-titative assessment of G.elegans alkaloid poisoning incidents.展开更多
文摘Crotalaria ferruginea Graham ex Benth.is a commonly used herb among ethnic minorities.Its whole plant is used as medicine for conditions such as heatstroke,tinnitus,hearing loss,phlegm heat cough,gum swelling and pain,lower back and knee pain,vaginal discharge and infantile malnutrition.Modern pharmaceutical research has found that Crotalaria ferruginea Graham ex Benth.mainly contains flavonoids,steroids,organic acids and terpenes,which have antibacterial,free radical scavenging,antiinflammatory and other effects.Quality control mainly focuses on characteristics,identification,content determination,etc.This article summarizes the recent research progress on the chemical composition,pharmacological effects,quality control and extraction process of Crotalaria ferruginea Graham ex Benth.on the basis of existing Crotalaria ferruginea Graham ex Benth.research reports.
基金Supported by the Special Science Foundation of Jiangxi Province(20143ACF60015)National Natural Scientific Fund(31360472)~~
文摘[Objective] The variation of sugar, acid and AsA contents in fruits of ‘Ganmi 6＇ kiwifruit （Actinidia eriantha Benth） were investigated during fruit develop- ment. [Method] Kiwi fruits were randomly taken as materials every 15 days since 20 days after full bloom （DAFB） to 170 DAFB until in mature stage. [Result] The results showed that during fruit development of ‘Ganmi 6＇, the total sugar soluble contents had a rising trend with relatively stable at 95 DAFB, then rose until har- vest with the maximum content （10.35%）. The titratable acid content showed a trend of increasing, then declining, then increase to the harvest content （1.10%）. From the sugar acid ratio, we can knew it decreased in 95 DAFB, then up to the max （9.38）. The changes of AsA contents showed double ‘S＇ shape, decreased af- ter the first increased rapidly, slightly increased and then decreased in the early harvest. [Conclusion] It provided a theoretical basis for scientific cultivation methods to explore the nutrients regulation.
基金Supported by the"Twelfth Five-Year"Key Project for Crop Breeding of Sichuan Province(YZGG4-6)the Special Support Program for the Scientific Research Personnel of Sichuan Agricultural University~~
文摘[Objective] This study aimed to understand the characters of hard seeds of Amphicarpaea edgeworthii Benth. （Leguminosae） and explore the methods for break- ing dormancy. [Method] For both aerial and subterranean seeds of A. edgeworthii, the morphological characteristics were observed and the appropriate temperature for germination was explored. For aerial seeds of A. edgeworthii, the characters of hard seeds were studied, and concentrated sulfuric acid treatment, hot water soak treat- ment and mechanical damage treatment were compared to explore the methods for breaking seed dormancy. [Result] The aerial seeds were oblate and averaged 3.38 mm in length, 3.02 mm in width, 1.88 mm in thickness, 15.32 g in thousand grain weight, with a hard seed rate after of up to 98% natural maturation. The subter- ranean seeds were approximately oblate with a maximum diameter of 15 mm and a hundred grain weight of （50.08-58.26 g）; among all the treatments for breaking hardseededness, cutting seed coat treatment and concentrated sulfuric acid treatment for 20 minutes were the most effective methods, whereas hot water soak treatment was the least effective method; constant temperature between 20 and 30 ℃ was optimum for the germination of aerial seeds, and alternative temperature of 30/20 ℃ was most appropriate for the germination of subterranean seeds. [Conclusion] Cutting seed coat treatment and concentrated sulfuric acid treatment for 20 minutes were the most effective methods to break the hardseededness of A. edgeworthii.
基金supported by the National Natural Science Foundation of China(NSFC:41801027)the National Natural Science Foundation of China(NSFC:3170043).
文摘To explore the optimum temperature,light intensityand water conditions for seed germination of Siphonostegia chinensis Benth.,seed germination experiment were carried out under different temperatures(5/15,10/20,15/25,20/30℃),different light intensity(14h light/10h darkness,complete darkness)and different concentrations(0%,5%,10%,15%,20%)of PEG-6000 solution.In terms of concentration,5%PEG was regarded as the low level,10%and 15%as the medium level,and 20%as the high level.The results showed that(1)Germination rate,germination potential,and germination index were increased with the rise of temperature.In addition,seed germination was significantly higher under the dark conditions than that with the 14h light/10h darkness.(2)No seed germination occurred when the temperature was below 10/20 C at 14h light/10h darkness.(3)Under 14h light/10h darkness,the germination rate,germination potential and germination index first increased and then decreased with the increase of PEG concentration.The low concentration was more beneficial to the seed germination.(4)Under the condition of complete darkness,the germination rate,germination potential and germination index decline with fluctuation with the increase of PEG concentration.Seed germination of Siphonostegia chinensis Benth.was inhibited in high concentration of PEG.
文摘Objective To establish an RP-HPLC method for the determination of β-Sitosterol in Elaeagnus Gonyanthes Benth.Methods The separation was performed on a luna C8(2)(150 mm×4.6 mm,5μm)column with the mobile phase of methanol-water(88∶12,v/v)at a flow rate of 1.0 mL/min,the detection wavelength was set at 210 nm,and the temperature of the column was maintained at 35 ℃.Results The calibration curve of β-Sitosterol was linear over the concentration range of 0.075-0.375 mg/mL(r=0.9999)and the average recovery of β-Sitosterol was 96.30% with RSD of 3.60%(n=3).Conclusion The method is simple,rapid,and accurate,and can be used for the quality control of Elaeagnus Gonyanthes Benth.
基金Supported by Guangxi Science and Technology Major Project(GK AA17202027-4)Key R&D Program of Guangxi Science and Technology Department(GK AB16380087)+2 种基金Tengxian Pueraria thomsonii Benth Experimental Station Project of Guangxi(G TS201431)Special Fund for Basal Scientific Research of Guangxi Academy of Agricultural Sciences(GNK 2019M16)Nanning Science and Technology Planning Project(20192085)。
文摘The cultivation technology of selenium-containing plant Pueraria thomsonii Benth was summarized from the producing area conditions,variety selection,field management and other aspects,which is of great significance for increasing the selenium concentration in humans or animals.
基金Collaborative Innovation in Tibetan Medicine(2019XTC013)Open Laboratory of Key Laboratory of Tibetan Medicine Basic Education(ZYYJC-19-01).
文摘[Objectives]To determine active components of Lamiophlomis rotata(Benth.)Kudo.[Methods]A new method was developed for the quantitative determination of L.rotata by using Ultra-performance convergence chromatography.The separation of active components of L.rotata was successfully achieved on Waters ACQUITY UPC column,with gradient elution carbon dioxide and methanol solvent at flow rate of 0.7 mL/min.The separation gradient elution rate was as follows:15%methanol 0-1 min,18%methanol 1-5 min,20%methanol 5-6 min,25%methanol 6-7 min,30%methanol 7-8 min,30%methanol 8-9 min,15%methanol 9-10 min.The conditions used in the separation process was as follows:column temperature 30℃;injection volume 2μL.Under the abovementioned conditions,the content of methyl glucoside was measured by 3D-full wavelength scanning.The validity of the method was tested by analyzing the precision,reproducibility,stability and accuracy.[Results]The results indicated that in terms of extraction and separation of compounds,supercritical CO2 not only has the diffusion property of gas,but also has the density and fluidity of liquid.[Conclusions]The ultra-high performance chromatography is a new technique for drug analysis.
文摘The volatile constituents of the essential oil of Hymenocrater longiflorus Benth. growing wild in Kurdistan-Iran were investigated using the GC and GC/MS techniques. Fifteen compounds, representing twenty (97.03%) of the total oil were identified. The main components were: α- Pinene (22.47%), β-Caryophyllene (18.05%), β- Eudesmol (14.92%), α-Copaene (9.84%), γ-Elemene (6.79%), δ-Cadine-ne (6.13%), (–)Bornyl acetate (5.61%), α-Amorphene (3.84%), α-Fen- chyl acetate (2.35%) and β-Pinene (2.07%). Some of the physicochemical properties like the logarithm of calculated Octanol-Water partitioning coefficients (log Kow), total biodegradation and (TBd in mol/h and gr./h), water solubility (Sw, mg.L-1 at 25oC) and median lethal concentration 50 (LC50) were calculated for the 15 com- ponents of Hymenocrater longiflorus Benth.
基金Supported by the Open Lab Project and Innovative Entrepreneurial Project of Institute of Chemical Engineering,Northwest University for NationalitiesFundamental Research Funds for the Central Universities( ZYZ2011063)
文摘[Objective] The study aims to optimize the extracting technology conditions of essential oil from Elsholtzia densa Benth. [Method] Essential oil was extracted from E. densa Benth using steam distillation, and the effects of four extracting factors (grain size of the medicinal herb, ultrasonic time, soaking time and ratio of the material to liquid) on the yield of essential oil extracted from E. densa Benth were analyzed through the orthogonal test. [Result] The optimized extraction process of essential oil from E. densa Benth was as follows: grain size of the medicinal herb 50 mesh, ultrasonic time 12 min, being soaked in the water for 6 h, and ratio of the material to liquid 1∶16 g/ml. Under the conditions, the average extracting rate of the essential oil was 0.407%. [Conclusion] The optimized extraction process of essential oil from E. densa Benth was simple and practicable, and its extracting rate was higher.
文摘The twigs and leaves of Pithecellobium clypearia(Jack.)Benth are used as common Chinese traditional herbal medicine to treat upper respiratory tract infection,acute gastroenteritis and bacterial dysentery.The study isolated nine compounds from the EtOAc extract,all of which were obtained from Pithecellobium Mart.for the first time.Based on 1H-NMR and 13C-NMR data,the structures of these compounds,including two ladiosiplodins and two dihydroisocoumarins,were elucidated.
基金Supported by the Innovation Project of Guangxi Graduate Education(No.YCSW2023388)Guangxi University of Chinese Medicine PhD Start-up Fund Project(No.2020BS034)。
文摘Objective:To evaluate the anti-hepatocellular carcinoma(HCC)activity of total alkaloids from Gelsemium elegans Benth.(TAG)in vivo and in vitro and to elucidate their potential mechanisms of action through transcriptomic analysis.Methods:TAG extraction was conducted,and the primary components were quantified using high-performance liquid chromatography(HPLC).The effects of TAG(100,150,and 200μg/mL)on various tumor cells,including SMMC-7721,HepG2,H22,CAL27,MCF7,HT29,and HCT116,were assessed.Effects of TAG on HCC proliferation and apoptosis were detected by colony formation assays and cell stainings.Caspase-3,Bcl-2,and Bax protein levels were detected by Western blotting.In vivo,a tumor xenograft model was developed using H22 cells.Totally 40 Kunming mice were randomly assigned to model,cyclophosphamide(20 mg/kg),TAG low-dose(TAG-L,0.5 mg/kg),and TAG high-dose(TAG-H,1 mg/kg)groups,with 10 mice in each group.Tumor volume,body weight,and tumor weight were recorded and compared during 14-day treatment.Immune organ index were calculated.Tissue changes were oberseved by hematoxylin and eosin staining and immunohistochemistry.Additionally,transcriptomic and metabolomic analyses,as well as quatitative real-time polymerase chain reaction(RT-qPCR),were performed to detect mRNA and metabolite expressions.Results:HPLC successfully identified the components of TAG extraction.Live cell imaging and analysis,along with cell viability assays,demonstrated that TAG inhibited the proliferation of SMMC-7721,HepG2,H22,CAL27,MCF7,HT29,and HCT116 cells.Colony formation assays,Hoechst 33258 staining,Rhodamine 123 staining,and Western blotting revealed that TAG not only inhibited HCC proliferation but also promoted apoptosis(P<0.05).In vivo experiments showed that TAG inhibited the growth of solid tumors in HCC in mice(P<0.05).Transcriptomic analysis and RT-qPCR indicated that the inhibition of HCC by TAG was associated with the regulation of the key gene CXCL13.Conclusion:TAG inhibits HCC both in vivo and in vitro,with its inhibitory effect linked to the regulation of the keygene CXCL13.
基金General Program of Guangxi Natural Science Foundation(No.2020GXNSFAA259076)Guangxi Science and Technology Project(No.AA17202040)Guangxi Traditional Chinese Medicine Key Discipline Construction Project(No.GZxk-z-20-75)。
文摘Objective To explore the antitumor effects of ethanol extract from Ventilago leiocarpa Benth(EEVLB)on sarcoma 180(S180)tumor-bearing mice and the potential mechanism.Methods Sixty mice were randomly assigned to 6 groups according to a random number table:normal group,model group,5-fluorouracil(5-FU)group(0.02 g·kg^(−1)),and high-,medium-,low-dose EEVLB groups(100,84,and 56 g of raw material·kg^(−1)body weight,respectively),with 10 mice each group.All treatments were given once daily for 10 consecutive days.Effects of EEVLB on inhibiting tumor growth and immune function in mice were evaluated among all groups after the treatments by detecting tumor inhibition rate,organ index,serum levels of interleukin(IL)-2,-6,-10,CD3^(+)CD4^(+)T lymphocytes,CD4^(+)/CD8^(+)ratio,caspase-3 and Bcl-2.Results EEVLB with different concentrations achieved inhibition of tumor growth in vivo,wherein the high-dose group showed the most significant reduction in tumor weight and increased apoptosis of tumor cells(P<0.05).In addition,both net weight gain and spleen index of mice showed uptrend in EEVLB treatment groups(P<0.05).Besides,serum levels of IL-2 and IL-6,percentages of CD3^(+)CD4^(+)T lymphocytes and ratio of CD4^(+)/CD8^(+)in peripheral blood were elevated in high-and medium-dose EEVLB groups compared with the model group(P<0.05).Also,upregulation of caspase-3 and downregulation of Bcl-2 were observed at protein levels in the high-dose EEVLB group(P<0.01).Conclusions EEVLB exhibits promising antitumor activity in vivo.This effect might be due to activation of apoptotic signaling pathway,increase of cytokine levels and enhancement of immune function in tumor-bearing mice.
基金supported by the National Natural Science Foundation of China(Grant No.32372448)Guangdong Basic and Applied Basic Research Foundation,China(Grant No.2023A1515012605)+2 种基金the Science and Technology Program of Guangdong Administration for Market Regulation,China(Grant No.2023CS01)the Science and Technology Program of National General Customs Administration of China(Grant No.2022HK108)the Science and Technology Program of Shantou City,China(Grant No.STKJ2023024).
文摘Gelsemium elegans Benth alkaloids are the main components of G.elegans and can cause acute toxicosis or even death.Although several studies have reported methods for detecting G.elegans alkaloids,a high-throughput and environmental-friendly strategy for detection of multiple G.elegans alkaloids has not been realized.In this work,a matrix-assisted laser desorption/ionization time-of-flight mass spectrometry method was developed for rapid detection of G.elegans alkaloids in human plasma and urine for diagnosis of poisoning.Multiple matrices and crys-tal spotting methods were evaluated to obtain stable and high peak intensities without“sweet spot”.We verified the methodology and obtained excellent results.The matrix effects with different dilutions were compared and good recoveries and a low relative standard deviation were obtained with a 40-fold dilution.This method could shorten the analysis time and greatly reduce the consumption of chemical solvents.Furthermore,it could be applied to quan-titative assessment of G.elegans alkaloid poisoning incidents.
