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CXCR4/SDF-1轴调节人肺腺癌PC-9细胞对Bends细胞血脑屏障模型功能的影响 被引量:5
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作者 李鸿茹 涂洵崴 +2 位作者 陈正伟 陈愉生 韩莉莉 《中国肿瘤生物治疗杂志》 CAS CSCD 北大核心 2020年第5期528-533,共6页
目的:通过建立体外血脑屏障(blood brain barrier,BBB)模型,探讨人肺腺癌PC-9细胞在CXCR4/SDF-1轴作用下对BBB紧密连接蛋白的影响。方法:利用永生化的小鼠脑微血管内皮细胞Bends进行单层培养,建立体外BBB模型;通过跨内皮细胞电阻(transe... 目的:通过建立体外血脑屏障(blood brain barrier,BBB)模型,探讨人肺腺癌PC-9细胞在CXCR4/SDF-1轴作用下对BBB紧密连接蛋白的影响。方法:利用永生化的小鼠脑微血管内皮细胞Bends进行单层培养,建立体外BBB模型;通过跨内皮细胞电阻(transendothelial electrical resistance,TEER)测定及荧光素钠通透性实验判定体外BBB模型的功能状态以及观察PC-9细胞对体外BBB模型功能的影响。Western blotting检测PC-9细胞在CXCR4抑制剂AMD3100、SDF-1单独或联合(1μg/ml AMD3100,100 ng/ml SDF-1,AMD3100+SDF-1)作用下对BBB模型功能和内皮细胞紧密连接蛋白表达的影响,Transwell迁移实验检测CXCR4/SDF-1轴对PC-9细胞跨BBB模型细胞层迁移能力的影响。结果:Bends细胞单层培养可形成紧密连接的"屏障"并产生较高的TEER,第96 h达到(182.13±5.19)Ω·cm^2;同时行荧光色钠通透性实验结果显示,BBB具有良好屏障性能,其通透率低于空白对照组(P<0.05)。PC-9细胞作用后,BBB模型TEER逐渐降低,第24 h降至(46.7±4.35)Ω·cm^2;同时BBB通透率较作用前显著提高(P<0.05)。PC-9细胞在AMD3100作用下能够上调内皮细胞紧密连接蛋白的表达(P<0.05);AMD3100处理组的PC-9细胞穿过BBB的细胞数较空白组明显减少[(43±2)vs(81±2)个,P<0.05]。结论:AMD3100能够减弱PC-9细胞对Bends细胞建立的体外BBB模型紧密连接的破坏能力。 展开更多
关键词 肺腺癌 PC-9细胞 血脑屏障 bends细胞 紧密连接 跨内皮细胞电阻 迁移 CXCR4/SDF-1轴
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Peculiarities of Normative Documentation Regulating the Production of Cold Bends
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作者 Nesterov Grigory Valerievich Yushin Alexey Alexandrovich +1 位作者 Glukhov Maksim Grigorievich Tkachuk Maxim Alexandrovich 《China Oil & Gas》 2025年第5期75-79,共5页
In the practice of pipeline transport of oil,oil products and gas to perform turns of trunk and field pipelines in vertical and horizontal planes are used bends of various designs such as:elbows with bending radius of... In the practice of pipeline transport of oil,oil products and gas to perform turns of trunk and field pipelines in vertical and horizontal planes are used bends of various designs such as:elbows with bending radius of 1.0DN or 1.5DN and standardized angles of 30°,45°,60°,90°;induction bends with bending angles from 1°to 90°;cold bends with bending angles depending on the nominal diameter DN. 展开更多
关键词 bending radius field pipelines trunk field pipelines gas ELBOWS pipeline transport oil products trunk pipelines
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Prediction of nanoparticle transport and deposition in bends
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作者 林培锋 林建忠 《Applied Mathematics and Mechanics(English Edition)》 SCIE EI 2009年第8期957-968,共12页
Nanoparticle transport and deposition in bends with circular cross-section are solved for different Reynolds numbers and Schmidt numbers. The perturbation method is used in solving the equations. The results show that... Nanoparticle transport and deposition in bends with circular cross-section are solved for different Reynolds numbers and Schmidt numbers. The perturbation method is used in solving the equations. The results show that the particle transport patterns are similar and independent of the particle size and other parameters when suspended nanoparticles flow in a straight tube. At the outside edge, particle deposition is the most intensive, while deposition at the inside edge is the weakest. In the upper and lower parts of the tube, depositions are approximately the same for different Schmidt numbers. Curvatures of tube, Reynolds number, and Schmidt number have second-order, forth-order, and first-order effects on the relative deposition efficiency, respectively. 展开更多
关键词 NANOPARTICLES TRANSPORT DEPOSITION perturbation method bends
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Nanoparticle Transport and Coagulation in Bends of Circular Cross Section via a New Moment Method 被引量:4
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作者 林建忠 林培锋 +1 位作者 于明州 陈华军 《Chinese Journal of Chemical Engineering》 SCIE EI CAS CSCD 2010年第1期1-9,共9页
Transport of nanoparticles and coagulation is simulated with the combination of CFD in a circular bend. The Taylor-expansion moment method(TEMOM)is employed to study dynamics of nanoparticles with Brownian motion,base... Transport of nanoparticles and coagulation is simulated with the combination of CFD in a circular bend. The Taylor-expansion moment method(TEMOM)is employed to study dynamics of nanoparticles with Brownian motion,based on the flow field from numerical simulation.A fully developed flow pattern in the present simulation is compared with previous numerical results for validating the model and computational code.It is found that for the simulated particulate flow system,the particle mass concentration,number concentration,particle polydispersity, mean particle diameter and geometric standard deviation over cross-section increase with time.The distribution of particle mass concentration at different time is independent of the initial particle size.More particles are concen-trated at outer edge of the bend.Coagulation plays more important role at initial stage than that in the subsequent period.The increase of Reynolds number and initial particle size leads to the increase of particle number concentration.The particle polydispersity,mean particle diameter and geometric standard deviation increase with decreasing Reynolds number and initial particle size. 展开更多
关键词 NANOPARTICLE COAGULATION transport flow in bend moment method
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Numerical Investigation of a New Method for Reducing Bends Erosion from Particles Impacts 被引量:2
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作者 姚军 樊建人 岑可法 《Chinese Journal of Chemical Engineering》 SCIE EI CAS CSCD 2002年第1期93-101,共9页
The present paper intends to introduce a new method for reducingbends erosion from particles impacts: the ribbed bend erosionprotection method. Ribs are evenly fixed in the range of 20 deg-80deg on the inner-wall of i... The present paper intends to introduce a new method for reducingbends erosion from particles impacts: the ribbed bend erosionprotection method. Ribs are evenly fixed in the range of 20 deg-80deg on the inner-wall of inside 90 deg bend and the bend (includingribs) is made of medium carbon steel. Three-dimensional numericalworks is performed and the result shows satisfactory agreement withthe experimental measurement. Numerical simulation studies thecharacteristics of axial gas flow along the bend and secondary flowat cross section. 展开更多
关键词 bend erosion particle impact RIB numerical simulation
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Numerical modeling of flow in continuous bends from Daliushu to Shapotou in Yellow River 被引量:2
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作者 He-fang JING Chun-guang LI +2 位作者 Ya-kun GUO Li-jun ZHU Yi-tian LI 《Water Science and Engineering》 EI CAS CSCD 2014年第2期194-207,共14页
The upper reach of the Yellow River from Daliushu to Shapotou consists of five bends and has complex topography. A two-dimensional Re-Normalisation Group (RNG) k-ε model was developed to simulate the flow in the re... The upper reach of the Yellow River from Daliushu to Shapotou consists of five bends and has complex topography. A two-dimensional Re-Normalisation Group (RNG) k-ε model was developed to simulate the flow in the reach. In order to take the circulation currents in the bends into account, the momentum equations were improved by adding an additional source term. Comparison of the numerical simulation with field measurements indicates that the improved two-dimensional depth-averaged RNG k-e model can improve the accuracy of the numerical simulation. A rapid adaptive algorithm was constructed, which can automatically adjust Manning's roughness coefficient in different parts of the study river reach. As a result, not only can the trial computation time be significantly shortened, but the accuracy of the numerical simulation can also be greatly improved. Comparison of the simulated and measured water surface slopes for four typical cases shows that the longitudinal and transverse slopes of the water surface increase with the average velocity upstream. In addition, comparison was made between the positions of the talweg and the main streamline, which coincide for most of the study river reach. However, deviations between the positions of the talweg and the main streamline were found at the junction of two bends, at the position where the river width suddenly decreases or increases. 展开更多
关键词 numerical simulation RNG k-e model Yellow River continuous bend circulationflow adaptive algorithm regarding Manning's roughness coefficient
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Effects of Hot Bending Parameters on Microstructure and Mechanical Properties of Weld Metal for X80 Hot Bends 被引量:1
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作者 Xu WANG Bo LIAO +3 位作者 Da-yong WU Xiu-lin HAN Yuan-sheng ZHANG Fu-ren XIAO 《Journal of Iron and Steel Research International》 SCIE EI CAS CSCD 2014年第12期1129-1135,共7页
Mechanical properties of weld metal are the key factors affecting the quality of heavy-wall X80 hot induction bends. The effects of bending parameters on the mechanical properties of weld metal for hot bends were inve... Mechanical properties of weld metal are the key factors affecting the quality of heavy-wall X80 hot induction bends. The effects of bending parameters on the mechanical properties of weld metal for hot bends were investigated by simulation conducted on a Gleeble 3500 thermal simulator. Continuous cooling transformation (CCT) dia- grams of the weld metal were also constructed. The influences of hot bending parameters (such as reheating temperature, cooling rate, and tempering temperature) on the microstructure and mechanical properties of weld metal were also analyzed. Results show that the strength of all weld metal specimens is higher than the value indicated in the technical specification and increases with the increase of reheating temperature, cooling rate, and tempering tempera ture. The impact toughness is apparently related to the variation of reheating temperature, cooling rate, and tempering temperature. 展开更多
关键词 hot induction bend X80 steel weld metal heat treatment microstructure
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Incipient Velocity of Non-uniform Sediment in Sloping River Bends 被引量:1
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作者 吴岩 徐海珏 +1 位作者 许栋 白玉川 《Transactions of Tianjin University》 EI CAS 2014年第3期163-167,共5页
A model for incipient movement of sediment in rolling pattern was established. In this model, the starting of sediment particles under low transport rate, the exposure degree of sediment, the lateral slope of water su... A model for incipient movement of sediment in rolling pattern was established. In this model, the starting of sediment particles under low transport rate, the exposure degree of sediment, the lateral slope of water surface and the effect of transverse circulating current induced by the hydraulic structure of bend flow were fully considered. A theoretical formula for the incipient velocity of non-cohesive and non-uniform sediment in sloping river bends was developed. The results from the theoretical formula compared well with the experimental data. 展开更多
关键词 sloping river bend non-uniform sediment exposure degree low transport rate of starting
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Simulation of subcritical flow pattern in 180° uniform and convergent open-channel bends using SSIIM 3-D model 被引量:1
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作者 Rasool GHOBADIAN Kamran MOHAMMADI 《Water Science and Engineering》 EI CAS 2011年第3期270-283,共14页
In meandering rivers, the flow pattern is highly complex, with specific characteristics at bends that are not observed along straight paths. A numerical model can be effectively used to predict such flow fields. Since... In meandering rivers, the flow pattern is highly complex, with specific characteristics at bends that are not observed along straight paths. A numerical model can be effectively used to predict such flow fields. Since river bends are not uniform-some are divergent and others convergent-in this study, after the SSIIM 3-D model was calibrated using the result of measurements along a uniform 180° bend with a width of 0.6 m, a similar but convergent 180v bend, 0.6 m to 0.45 m wide, was simulated using the SSI1M 3-D numerical model. Flow characteristics of the convergent 180° bend, including lengthwise and vertical velocity profiles, primary and secondary flows, lengthwise and widtbwise slopes of the water surface, and the helical flow strength, were compared with those of the uniform 180° bend. The verification results of the model show that the numerical model can effectively simulate the flow field in the uniform bend. In addition, this research indicates that, in a convergent channel, the maximum velocity path at a plane near the water surface crosses the channel's centerline at about a 30° to 40° cross-section, while in the uniform bend, this occurs at about the 50° cross-section. The varying range of the water surface elevation is wider in the convergent channel than in the uniform one, and the strength of the helical flow is generally greater in the uniform channel than in the convergent one. Also, unlike the uniform bend, the convergent bend exhibits no rotational cell against the main direction of secondary flow rotation at the 135° cross-section. 展开更多
关键词 flow pattern numerical simulation convergent 180° bend SSIIM 3-D model
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Effect of cross-section distortion on the performance of small-radius induction bends
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作者 Wang Gaofeng Yang Zhuanzhao +3 位作者 Liu Yinglai Nie Xianghui Xu Yan Zhao Jinlan 《Natural Gas Industry B》 2019年第1期51-57,共7页
While a small-radius induction bend is manufactured,various cross-section distortions may occur,such as its cross section tending to be elliptic,and wall thickness reduction or increase.These defects may deteriorate t... While a small-radius induction bend is manufactured,various cross-section distortions may occur,such as its cross section tending to be elliptic,and wall thickness reduction or increase.These defects may deteriorate the bearing capacity of pipelines.In order to study the effect of cross-section distortion on the performance of small-radius induction bends,3D(D refers to pipe diameter)induction bends were manufactured based on different combinations of process parameters.Then,the cross-section distortion,structure properties and their correlation of 3D bends and conventional 5D bends were analyzed comprehensively by means of cross-section geometry analysis,distortion analysis,physical and chemical property test,micro-structural analysis and so on.The following results were obtained.First,the wall thickening/thinning rate of 3D bends is about 2 times that of 5D bends,and the roundness of 3D bends is about 2e4 times that of 5D bends.Second,the cross-section distortion of 3D bends is obvious,so its heating temperature varies greatly in different parts,leading to different structure morphologies and contents.As a result,the numerical distribution of mechanical properties of each part of 3D bends(e.g.intrados,extrados and neutral zone)is discrete.Third,the strength at the intrados of most bends doesn't meet the standard,and it is sorted form the higher to the lower as extrados,neutral zone and intrados while the sequence of Charpy impact toughness is right contrary to the strength.Fourth,the strength at intrados and extrados decreases with the increase of roundness.The strength at intrados decreases with the increase of thickening rates.And the strength at extrados increases with the increase of thinning rates. 展开更多
关键词 Small-radius induction bend Cross-section distortion Induction coil Intrados Extrados Neutral zone Strength Roundness Thickening rate Thinning rate
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NUMERICAL MODELING OF SUSPENDED SEDIMENT TRANSPORT IN CHANNEL BENDS 被引量:12
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作者 HUANG Sui-liang JIAY.F,WANG Sam S. Y. 《Journal of Hydrodynamics》 SCIE EI CSCD 2006年第4期411-417,共7页
An algorithm to compute three-dimensional sediment transport effect was proposed in this paper to enhance the capability of depth-averaged numerical models. This algorithm took into account of non-uniform distribution... An algorithm to compute three-dimensional sediment transport effect was proposed in this paper to enhance the capability of depth-averaged numerical models. This algorithm took into account of non-uniform distributions of flow velocities and suspended sediment concentrations along water depth, it significantly enhanced the applicability of 2D models in simulating open channel flows, especially in channel bends. Preliminary numerical experiments in a U-shaped and a sine-generated experimental channel indicate that the proposed method performs quite well in predicting the change of bed-deformation in channel bends due to suspended sediment transport. This method provides an effective alternative for the simulations of channel morphodynamic changes. 展开更多
关键词 ALGORITHM dispersion terms bends suspended sediment transport 2D numerical model
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COMPUTATION OF TURBULENT FLOW AND HEAT TRANSFER IN LARGE RADIUS 90-DEGREE CIRCULAR BENDS
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作者 M. J. Wang(Technische Universitat Munchen, BRD)Su Ming-de(Tsinghua University, Beijing, 100084, P. R. China) 《Journal of Hydrodynamics》 SCIE EI CSCD 1994年第1期91-102,共12页
A numerical study of fluid flow and heat transfer of turbulent developing flow in 90°circular bends is presented using a finite-difference method bond CFD code. Results show an appreciable fluid redistribution pr... A numerical study of fluid flow and heat transfer of turbulent developing flow in 90°circular bends is presented using a finite-difference method bond CFD code. Results show an appreciable fluid redistribution process in large radius bend that brings about an overall heat transfer augmentation, especially at the outer side of the bend, and the significant deterioration at the inner side. The influence of two major parameters, namely, the bend-to-tube radius ratio and the bulk Reynolds number on the flow structure as well as on the local heat transfer is investigated. 展开更多
关键词 turbulent now heat transfer circular bends numerical study
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THE GAS-PARTICLE FLOW AND EROSION IN BENDS WITH A RECTANGULAR CROSS-SECTION
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作者 Zhang Jing-ping Zhang Ben-zhao Shen Xin-rong 《Journal of Hydrodynamics》 SCIE EI CSCD 1999年第1期64-72,共9页
In this paper, the computational results of a gas-particle two phase flow in a bend with a rectangular cross-section is presented. The movement of the particles in a curved pipe is numerically analyzed by using random... In this paper, the computational results of a gas-particle two phase flow in a bend with a rectangular cross-section is presented. The movement of the particles in a curved pipe is numerically analyzed by using random statistical method. The zones and rate of erosion on the wall in a bend are determined. The results are in accordance with the experiment, which indicate that the erosion is influenced by gas velocity, particle size and bend curvature. 展开更多
关键词 two phase flow in bends. erosion application of tensor
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Characterization of the accommodation zones along restraining and releasing bends from analogue modelling simulating the seagap fault,off-shore Tanzania
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作者 Nasra Sadiki Godson Godfray Michael Msabi 《Petroleum Research》 2021年第4期431-442,共12页
The study aims to understand geometry and evolution of accommodation zones along restraining and releasing bends in strike slip systems which is often complex and characterized by sharp change in polarity,resulting in... The study aims to understand geometry and evolution of accommodation zones along restraining and releasing bends in strike slip systems which is often complex and characterized by sharp change in polarity,resulting in a difficult characterization of traps location.A batch of sandbox modelling experiments was performed using a layered scaled sand material.The setup parameters of the wooden baseplates used in the first two experiments(SS1 and SS2)are basement offset of 6.5 cm/3 cm,90/90stepovers in releasing and restraining bends of total 96 cm length and 25 cm width.The third experiment(SS3)was performed with basement offset of 6.5 cm/3 cm,156/126stepovers in releasing and restraining bends of total 96 cm length and 25 cm width.The experiments were performed with special attention to the role of syn-kinematic sedimentation and the pre-existing structure of the basement.A sequence of pop-up and pull-apart structures was produced.Along the main structures,complex fault trend was compatible with a sinistral riedel distribution and a counterclockwise rotation of pre-existent elements.Change of fault's polarity at depth and dip direction flipping of high angle faults in accommodation zones were clearly observed.Even more,progressive propagation of the pop-up structures produces a spectacular inversion of adjacent extensional structures.Comparing results of the model with the Seagap fault zone,it is possible to put in evidence similar fault orientation and distribution.Internal structures developed were strongly influenced by syn-kinematic sedimentation as observed by characteristic helicoidal shape of growth faults.The analogue models clearly describe how boundary faults of the pull-apart basin along a paired bend,initially characterized by a normal kinematic can be subsequently inverted in the later stages,which can potentially have a strong impact on the petroleum system. 展开更多
关键词 Sandbox experiments Seagap faults Syn-kinematic sedimentation Restraining and releasing bends
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Sperm Whales Suffer the Bends
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作者 Michael Hopkin 席芳 《当代外语研究》 2005年第2期2-3,共2页
据原先推测,鲸鱼对由声纳系统导致的疾病具有免疫力,但是两位美国科学家发现,抹香鲸(Sperm whales)正遭受着骨坏死疾病的侵扰,这是由于声纳系统扰乱了鲸鱼的生活习性所致,这种疾病有可能导致它们的搁浅。鲸鱼亟需得到保护!
关键词 骨坏死 Sperm Whales Suffer the bends
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Energy loss at bends in the pneumatic conveying of fly ash 被引量:5
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作者 Naveen Tripathi Atul Sharma +1 位作者 S.S.Mallick P.W.Wypych 《Particuology》 SCIE EI CAS CSCD 2015年第4期65-73,共9页
An accurate estimation of the total pressure drop of a pipeline is important to the reliable design of a pneumatic conveying system. The present paper presents results from an investigation into the modelling of the p... An accurate estimation of the total pressure drop of a pipeline is important to the reliable design of a pneumatic conveying system. The present paper presents results from an investigation into the modelling of the pressure drop at a bend in the pneumatic conveying of fly ash. Seven existing bend models were used (in conjunction with solids friction models for horizontal and vertical straight pipes, and initial acceleration losses) to predict the total pipeline pressure drop in conveying fly ash (median particle diameter: 30 }zm; particle density: 2300 kg/m^3; loose-poured bulk density: 700 kg/m3) in three test rigs (pipelines with dimensions of 69 mm inner diameter (I.D.) × 168 m length; 105 mm I.D. × 168 m length; 69 mm I.D. × 554 m length). A comparison of the pneumatic conveying characteristics (PCC) predicted using the seven bend models and experimental results shows that the predicted total pipeline PCC and trends depend on the choice of bend model. While some models predict trends that agree with the experimental results, other models predicted greater bend pressure drops for the dense phase of fly ash than for the dilute phase. Models of Pan, R. (1992). Improving scale-up procedures for the design of pneumatic conveying systems. Doctoral dissertation, University of Wollongong, Australia, Pan, R., & Wypych, P.W. (1998). Dilute and dense phase pneumatic conveying of fly ash. In Proceedings of the sixth International Conference on Bulk Materials Storage and Transportation (pp. 183-189), Wollongong, NSW, Australia and Chambers, A.J., & Marcus, R.D. (1986). Pneumatic conveying calculations. In Proceedings of the second International Conference on Bulk Materials Storage and Transportation (pp. 49-52), Wollongong, Australia reliably predicted the bend losses for systems conveying fly ash over a large range of air flows. 展开更多
关键词 Pneumatic conveying Fluidised dense phase BEND Pressure drop Bend model
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PROGRESS IN STUDIES ON ICE ACCUMULATION IN RIVER BENDS 被引量:8
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作者 WANG Jun CHEN Pang-pang SUI Jue-yi 《Journal of Hydrodynamics》 SCIE EI CSCD 2011年第6期737-744,共8页
River ice is an important hydraulic element in temperate and polar environments and would affect hydrodynamic conditions of rivers through changes both in the boundary conditions and the thermal regime. The river bend... River ice is an important hydraulic element in temperate and polar environments and would affect hydrodynamic conditions of rivers through changes both in the boundary conditions and the thermal regime. The river bend has been reported as the common location for the initiation of ice jams because the water flow along a river bend is markedly affected by the channel curvature. In this article, the experimental studies about the ice accumulation in a river bend are reviewed. Based on experiments conducted so far, the criteria for the formation of ice jams in the river bend, the mechanisms of the ice accumulation in the river bend and the thickness profile of the ice accumulation in the river bend are discussed. The two-equation turbulence model is used to simulate the ice accumulation under an ice cover along a river bend. A formula is proposed for describing the deformation of the ice jam bottom. Our results indicate that all simulated thickness of the ice accumulation agrees reasonably well with the measured thickness of the ice accumulation in the laboratory. 展开更多
关键词 EXPERIMENT ice jam/accumulation bend channel thickness profile
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Numerical modelling of supercritical flow in circular conduit bends using SPH method
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作者 Nikola M.Rosie Milena B.Kolarevie +2 位作者 Ljubodrag M.Savie Dejana M.Doraevic Radomir S.Kapor 《Journal of Hydrodynamics》 SCIE EI CSCD 2017年第2期344-352,共9页
The capability of 1he smoothed-particle hydrodynamics (SPH) method to model supercritical flow in circular pipe bends is considered. The standard SPH method, which makes use of dynamic boundary particles (DBP), is... The capability of 1he smoothed-particle hydrodynamics (SPH) method to model supercritical flow in circular pipe bends is considered. The standard SPH method, which makes use of dynamic boundary particles (DBP), is supplemented with the original algorithm for the treatment of open boundaries. The method is assessed through a comparison with measured free-surface profiles in a pipe bend, and already proposed regression curves for eslimation of the flow-type in a pipe bend. The sensitivity of the model to different parameters is also evaluated. It is shown that an adequate choice of the artificial viscosity coefficient and the initial particle spacing can lead to correct presentation of the flow-type in a bend. Due to easiness of its implementation, the SPH method can he efficiently used in the design of circular conduits with supercritical flow in a bend, such as tunnel spillways, and bottom outlets of dams, or storm sewers. 展开更多
关键词 Smoothed-particle hydrodynamics (SPH) super critical bend flow helical flow artificial viscosity
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补阳还五汤通过小窝蛋白1调控YAP1/HIF-1α信号通路促进bEnd.3细胞糖氧剥夺/复氧损伤后血管新生的机制研究 被引量:2
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作者 陈博威 欧阳银 +5 位作者 曾繁佐 刘英飞 田丰铭 徐雅倩 易健 刘柏炎 《中国中药杂志》 北大核心 2025年第14期3847-3856,共10页
基于小窝蛋白1(Cav1)/Yes-相关蛋白同源癌蛋白1(YAP1)/缺氧诱导因子-1α(HIF-1α)信号通路探讨补阳还五汤促进小鼠脑微血管内皮细胞株(bEnd.3)糖氧剥夺/复氧(OGD/R)损伤后血管新生的作用机制。运用超高效液相色谱-四极杆-飞行时间质谱... 基于小窝蛋白1(Cav1)/Yes-相关蛋白同源癌蛋白1(YAP1)/缺氧诱导因子-1α(HIF-1α)信号通路探讨补阳还五汤促进小鼠脑微血管内皮细胞株(bEnd.3)糖氧剥夺/复氧(OGD/R)损伤后血管新生的作用机制。运用超高效液相色谱-四极杆-飞行时间质谱联用仪(UPLC-Q-TOF-MS)分析补阳还五汤入血成分。采用胆囊收缩素八肽(CCK-8)法检测bEnd.3细胞OGD/R损伤后补阳还五汤含药血清的最佳干预浓度。慢病毒转染的方式构建Cav1沉默稳转株,Western blot法及PCR法验证沉默效率。将对照bEnd.3细胞分为正常组(sh-NC control组)、OGD/R模型+空白血清组(sh-NC OGD/R组)及OGD/R模型+含药血清组(sh-NC BHD组),Cav1沉默细胞分为OGD/R模型+空白血清组(sh-Cav1 OGD/R组)及OGD/R模型+含药血清组(sh-Cav1 BHD组),通过CCK-8法检测细胞存活率,细胞迁移实验检测细胞迁移能力,管腔形成实验检测细胞成管能力,流式细胞术检测细胞凋亡率,Western blot法检测各组细胞YAP1/HIF-1α信号通路相关蛋白的表达情况,最后运用免疫共沉淀法验证YAP1与HIF-1α之间的互作关系。结果显示补阳还五汤中黄芪甲苷、芒柄花素、阿魏酸及芍药内酯苷均能入血。质量分数10%的补阳还五汤含药血清可能是OGD/R诱导损伤bEnd.3细胞的最佳干预浓度。与sh-NC control组比较,sh-NC OGD/R组细胞存活率、细胞迁移率、网眼数、节点数和管腔长度明显下降,细胞凋亡率明显上升,YAP1的S127位点磷酸化水平明显下降,YAP1的核位移水平与HIF-1α、血管内皮生长因子(VEGF)及VEGFR2的蛋白表达明显上升;与同类型OGD/R组比较,sh-NC BHD组及sh-Cav1 BHD组细胞存活率、细胞迁移率、网眼数、节点数和管腔长度明显升高,细胞凋亡率明显下降,YAP1的S127位点磷酸化水平进一步下降,YAP1的核位移水平与HIF-1α、血管内皮生长因子(VEGF)及VEGFR2的蛋白表达明显上升;与sh-NC OGD/R组比较,sh-Cav1 OGD/R组细胞存活率、细胞迁移率、网眼数、节点数和管腔长度明显下降,细胞凋亡率明显上升,YAP1的S127位点磷酸化水平明显上升,YAP1的核位移水平与HIF-1α、VEGF及VEGFR2的蛋白表达明显下降;与sh-NC BHD组比较,sh-Cav1 BHD组细胞存活率、细胞迁移率、网眼数、节点数和管腔长度明显下降,细胞凋亡率明显上升,YAP1的S127位点磷酸化水平明显上升,YAP1的核位移水平与HIF-1α、VEGF及VEGFR2的蛋白表达明显下降。在HIF-1α抗体沉淀的蛋白复合物中存在YAP1蛋白,而在YAP1抗体沉淀的蛋白复合物中亦存在HIF-1α蛋白。结论证实补阳还五汤含药血清能够通过Cav1提高OGD/R损伤bEnd.3细胞YAP1/HIF-1α通路的活性,促进体外血管新生。 展开更多
关键词 补阳还五汤 小窝蛋白1 YAP1/HIF-1α信号通路 bEnd.3细胞 糖氧剥夺/复氧损伤 血管新生
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含“益宫清”奶牛血清缓解奶牛子宫内膜上皮细胞炎症损伤的作用机制
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作者 于书凝 王建发 +3 位作者 连帅 解颖颖 武瑞 胡学权 《东北农业大学学报》 北大核心 2025年第2期80-88,共9页
选用大庆市杜尔伯特牛场奶牛,筛选“益宫清”治疗奶牛子宫内膜炎的最佳剂量,并制备含药血清。将含药血清作用于经LPS处理的奶牛子宫内膜上皮细胞(BEND),通过蛋白组学分析筛选差异蛋白,探究其作用机制。结果表明:高剂量组(质量浓度为220 ... 选用大庆市杜尔伯特牛场奶牛,筛选“益宫清”治疗奶牛子宫内膜炎的最佳剂量,并制备含药血清。将含药血清作用于经LPS处理的奶牛子宫内膜上皮细胞(BEND),通过蛋白组学分析筛选差异蛋白,探究其作用机制。结果表明:高剂量组(质量浓度为220 g·L^(-1))治疗效果最佳,并按此剂量制备含药血清;体外试验表明,益宫清可显著降低LPS诱导的BEND细胞炎症因子表达水平;蛋白组学分析结果共鉴定出7 342个相关差异蛋白;GO富集分析显示,差异蛋白主要富集在生物进程和抗氧化过程等;KEGG聚类分析显示,差异蛋白主要涉及PPAR、AMPK、NFκB等信号通路;验证差异显著蛋白NFκB2、NκIRAS2,与蛋白质组学结果一致,表明益宫清可能通过上调NFκB2及下调NκIRAS2蛋白发挥抗炎作用。 展开更多
关键词 益宫清 BEND细胞 蛋白质组学 炎症 作用机制
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