Objective\ To understand the transcription of BamHI L DNA fragment from genome of strong virulent GA strain of Marek′s disease herpesvirus (MDV) in lymphoblastoid tumor tissue induced by oncogenic strain Beijing 1 ...Objective\ To understand the transcription of BamHI L DNA fragment from genome of strong virulent GA strain of Marek′s disease herpesvirus (MDV) in lymphoblastoid tumor tissue induced by oncogenic strain Beijing 1 (a specific local strain in China) of MDV. Methods\ Two oligonucleotide primers were synthesized according to the reported sequence of \%meq\% gene an ideal oncogenic candidate and our previously determined sequence of BamHI L fragment of Marek′s disease herpesvirus (MDV), respectively. Reverse transcriptase PCR(RT PCR) assay was performed by using these primers and the mRNA as a template which was isolated from visceral lymphoblastoid tumors obtained from chickens artificially infected with strain Beijing 1 of oncogenic MDV. Southern blot molecular hybridization was further carried out to detect the product of RT PCR with digoxigenin labeled nucleotide probe from BamHI I2 and L fragment in the gene library of MDV strain GA, respectively. Results\ Two probes could simultaneously hybridize this cDNA amplified by RT PCR with a length of about 730 bp. Conclusion\ It is suggested that \%meq\% transcription could extend from the right hand end of BamHI I2 to the adjacent BamHI L, and the BamHI L region was likely to be transcribed in MDV induced lymphoblastoid tumors.展开更多
In order to promote the application of Beijing-1 small satellite(BJ-1) remote sensing data,the multispectral and panchromatic images captured by BJ-1 were used for land cover classification in Pangzhuang Coal Mining.A...In order to promote the application of Beijing-1 small satellite(BJ-1) remote sensing data,the multispectral and panchromatic images captured by BJ-1 were used for land cover classification in Pangzhuang Coal Mining.An improved Intensity-Hue-Saturation(IHS) fusion algorithm is proposed to fuse panchromatic and multispectral images,in which intensity component and panchromatic image are combined using the weights determined by edge pixels in the panchromatic image identified by grey absolute correlation degree.This improved IHS fusion algorithm outper-forms traditional IHS fusion method to a certain extent,evidenced by its ability in preserving spectral information and enhancing spatial details.Dempster-Shafer(D-S) evidence theory was adopted to combine the outputs of three member classifiers to generate the final classification map with higher accuracy than that by any individual classifier.Based on this study,we conclude that Beijing-1 small satellite remote sensing images are useful to monitor and analyze land cover change and ecological environment degradation in mining areas,and the proposed fusion algorithms at data and decision levels can integrate the advantages of multi-resolution images and multiple classifiers,improve the overall accuracy and produce a more reliable land cover map.展开更多
According to the features of high-resolution panchromatic imagery of Beijing-1 small satellite,an approach to extracting information of residential areas is proposed in this paper based on Gabor texture segmentation.T...According to the features of high-resolution panchromatic imagery of Beijing-1 small satellite,an approach to extracting information of residential areas is proposed in this paper based on Gabor texture segmentation.The algorithm extracts the features in different directions and different scales by building the Gabor filter,uses cluster analysis of multiple features to segment the image,and performs the fusion processing based on morphological scale space.It solves the problems in image processing resulting from low contrast between remote sensing objects and background,the blurring of image edges and high noise.It has the benefits of direction selection and frequency selection with strong self-adaptive ability.Our experiments prove the effectiveness of the approach for extracting information of residential areas from Beijing-1 high-resolution imagery.展开更多
These problems of nonlinearity, fuzziness and few labeled data were rarely considered in traditional remote sensing image classification. A semi-supervised kernel fuzzy C-means （SSKFCM） algorithm is proposed to over...These problems of nonlinearity, fuzziness and few labeled data were rarely considered in traditional remote sensing image classification. A semi-supervised kernel fuzzy C-means （SSKFCM） algorithm is proposed to overcome these disadvantages of remote sensing image classification in this paper. The SSKFCM algorithm is achieved by introducing a kernel method and semi-supervised learning technique into the standard fuzzy C-means （FCM） algorithm. A set of Beijing-1 micro-satellite＇s multispectral images are adopted to be classified by several algorithms, such as FCM, kernel FCM （KFCM）, semi-supervised FCM （SSFCM） and SSKFCM. The classification results are estimated by corresponding indexes. The results indicate that the SSKFCM algorithm significantly improves the classification accuracy of remote sensing images compared with the others.展开更多
文摘Objective\ To understand the transcription of BamHI L DNA fragment from genome of strong virulent GA strain of Marek′s disease herpesvirus (MDV) in lymphoblastoid tumor tissue induced by oncogenic strain Beijing 1 (a specific local strain in China) of MDV. Methods\ Two oligonucleotide primers were synthesized according to the reported sequence of \%meq\% gene an ideal oncogenic candidate and our previously determined sequence of BamHI L fragment of Marek′s disease herpesvirus (MDV), respectively. Reverse transcriptase PCR(RT PCR) assay was performed by using these primers and the mRNA as a template which was isolated from visceral lymphoblastoid tumors obtained from chickens artificially infected with strain Beijing 1 of oncogenic MDV. Southern blot molecular hybridization was further carried out to detect the product of RT PCR with digoxigenin labeled nucleotide probe from BamHI I2 and L fragment in the gene library of MDV strain GA, respectively. Results\ Two probes could simultaneously hybridize this cDNA amplified by RT PCR with a length of about 730 bp. Conclusion\ It is suggested that \%meq\% transcription could extend from the right hand end of BamHI I2 to the adjacent BamHI L, and the BamHI L region was likely to be transcribed in MDV induced lymphoblastoid tumors.
基金Under the auspices of National Natural Science Foundation of China (No. 40871195)Opening Fund of Beijing-1Small Satellite Data Applications from State Key Laboratory for Remote Sensing Science (No. 200709)National High Technology Research and Development Program of China (No. 2007AA12Z162)
文摘In order to promote the application of Beijing-1 small satellite(BJ-1) remote sensing data,the multispectral and panchromatic images captured by BJ-1 were used for land cover classification in Pangzhuang Coal Mining.An improved Intensity-Hue-Saturation(IHS) fusion algorithm is proposed to fuse panchromatic and multispectral images,in which intensity component and panchromatic image are combined using the weights determined by edge pixels in the panchromatic image identified by grey absolute correlation degree.This improved IHS fusion algorithm outper-forms traditional IHS fusion method to a certain extent,evidenced by its ability in preserving spectral information and enhancing spatial details.Dempster-Shafer(D-S) evidence theory was adopted to combine the outputs of three member classifiers to generate the final classification map with higher accuracy than that by any individual classifier.Based on this study,we conclude that Beijing-1 small satellite remote sensing images are useful to monitor and analyze land cover change and ecological environment degradation in mining areas,and the proposed fusion algorithms at data and decision levels can integrate the advantages of multi-resolution images and multiple classifiers,improve the overall accuracy and produce a more reliable land cover map.
基金supported by a grant from the National High Technology Research and Development Program of China(863 Program)(No.2005AA133013,No.2006CB701305)the Major State Basic Research Development Program of China(973 Program)(No.2006CB701305).
文摘According to the features of high-resolution panchromatic imagery of Beijing-1 small satellite,an approach to extracting information of residential areas is proposed in this paper based on Gabor texture segmentation.The algorithm extracts the features in different directions and different scales by building the Gabor filter,uses cluster analysis of multiple features to segment the image,and performs the fusion processing based on morphological scale space.It solves the problems in image processing resulting from low contrast between remote sensing objects and background,the blurring of image edges and high noise.It has the benefits of direction selection and frequency selection with strong self-adaptive ability.Our experiments prove the effectiveness of the approach for extracting information of residential areas from Beijing-1 high-resolution imagery.
基金Supported by the National High Technology Research and Development Programme （No.2007AA12Z227） and the National Natural Science Foundation of China （No.40701146）.
文摘These problems of nonlinearity, fuzziness and few labeled data were rarely considered in traditional remote sensing image classification. A semi-supervised kernel fuzzy C-means （SSKFCM） algorithm is proposed to overcome these disadvantages of remote sensing image classification in this paper. The SSKFCM algorithm is achieved by introducing a kernel method and semi-supervised learning technique into the standard fuzzy C-means （FCM） algorithm. A set of Beijing-1 micro-satellite＇s multispectral images are adopted to be classified by several algorithms, such as FCM, kernel FCM （KFCM）, semi-supervised FCM （SSFCM） and SSKFCM. The classification results are estimated by corresponding indexes. The results indicate that the SSKFCM algorithm significantly improves the classification accuracy of remote sensing images compared with the others.
