Purpose： The purpose of the study was to examine the psychometric properties of the Chinese-translated Behavioral Regulation in Exercise Questionnaire-2 （C-BREQ-2） among Chinese university students from the Mainlan...Purpose： The purpose of the study was to examine the psychometric properties of the Chinese-translated Behavioral Regulation in Exercise Questionnaire-2 （C-BREQ-2） among Chinese university students from the Mainland and Hong Kong of China. Methods： University students from the Mainland （n = 191） and Hong Kong （n=194） of China participated in this study. Factorial validity, discriminant validity, nomological validity, internal reliability, and measurement invariance across sample of the C-BREQ-2 were examined. Results： Confirmatory factor analysis provided support for the factorial validity of the 18-item, 5-factor structure C-BREQ-2. Examination of the 95% confidence interval of the inter-factor correlations suggested that the C-BREQ-2 assesses related but distinct constructs, which provided support for its discriminant validity. The internal consistency reliability of the C-BREQ-2 was found acceptable. Examination of the pattern of inter-factor correlations between different regulations suggested that a simplex-like pattern was displayed, which provided evidence for the nomological validity of C-BREQ-2. The results from multi-group confirmatory factor analysis suggested that the factor loadings and variances/ covariances of the C-BREQ-2 measurement model were invariant across the Chinese university students in the Mainland and Hong Kong of China. Conclusion： The current study provided further psychometric evidence for the C-BREQ-2, which makes the further application and research of self-determination theory （SDT） based motivation in relation to exercise and physical activity in the Mainland of China context possible.展开更多
The preoptic area （POA） is located in the most anterior part of the hypothalamus and is bordered dorsally by the anterior commissure and anteroventrally by the nucleus of the diagonal band of Broca[1]. Accumulating ...The preoptic area （POA） is located in the most anterior part of the hypothalamus and is bordered dorsally by the anterior commissure and anteroventrally by the nucleus of the diagonal band of Broca[1]. Accumulating evidence from developmental neurobiology suggests, however, that the POA may be a separate entity from hypothalamus, and may actually be part of the basal telencephalon[2,3]. Both the hypothalamus and POA are highly complex and heterogeneous areas, containing multiple nuclei, each of which has specific fundamental functions for survival. Among these, the POA contains nuclei involved in the regulation of blood osmolality and temperature （the median preoptic nucleus）, sleep （the ventrolateral preoptic and suprachiasmatic nuclei）, ovulation （gonadotropin-releasing hormone neurons scattered mainly in the ventral part of the POA）, male sexual behavior （the medial preoptic nucleus）, and parental behavior （the central part of the medial POA, cMPOA）.展开更多
Methyl-CpG binding protein 2 （MeCP2） has a crucial role in transcriptional regulation and neural development （Ausi6 et al., 2014）. Loss of function mutations of MECP2 in human lead to Rett syndrome （RTT）, a seve...Methyl-CpG binding protein 2 （MeCP2） has a crucial role in transcriptional regulation and neural development （Ausi6 et al., 2014）. Loss of function mutations of MECP2 in human lead to Rett syndrome （RTT）, a severe neurodevelopmental disorders （Amir et al., 1999）, whereas individuals with the chromosomal duplications containing the MECP2 locus showed severe autism-like symptoms （Ramocki et al., 2009）.展开更多
Sugar and protein are the major macronutrients＇ sources, and their balanced intake is important for animal＇s health. It has been observed that animals are able to change food preference in an imbalanced nutritional ...Sugar and protein are the major macronutrients＇ sources, and their balanced intake is important for animal＇s health. It has been observed that animals are able to change food preference in an imbalanced nutritional condition to selectively consume nutrients that are deficient in the body （Dethier, 1976）. Early studies in both Drosophila and mouse have demonstrated that animals exhibit food rejection to imbalanced diets lacking essential amino acids （Hao et al., 2005; Bjordal et al., 2014）. Furthermore, the food preference change upon protein depri- vation has been characterized using a two choice assay in Drosophila （Ribeiro and Dickson, 2010; Vargas et al., 2010）. Different from protein food, sugar is the main energy source, and sugar deficiency severely affects animal survival （Lee et al., 2008）. However, whether animals adopt a strategy of fast food preference switch upon sugar deprivation had not been investigated, and the neural mechanisms underlying this behavior regulation remain poorly understood.展开更多
Hyperactivity and tic symptoms in children and adolescents with Attention Deficit Hyperactivity Disorder(ADHD)have a bidirectional association with sleep disorders,while magnesium deficiency is a key nutritional facto...Hyperactivity and tic symptoms in children and adolescents with Attention Deficit Hyperactivity Disorder(ADHD)have a bidirectional association with sleep disorders,while magnesium deficiency is a key nutritional factor exacerbating this vicious cycle.The SLS Magnesium Glycinate Complex Liquid Supplement formula adopts the core logic of“sleep improvement-mood stabilization-behavioral regulation”and enhances the neuromodulatory effects of magnesium ions through multi-component synergy.Tailored to the developmental needs of different stages aged 4-16 years,the formula features a child-friendly dosage design,forming a closed-loop regulation in aiding sleep,reducing tics,and maintaining daytime functioning.A 3-month clinical trial showed that the formula improved sleep efficiency by 15%-23%,reduced tic frequency by 21%-32%,and enhanced emotional stability scores by 24%-30%across all age groups with ADHD,with good safety and tolerability.This study provides a targeted nutritional intervention solution for ADHD,and its age-stratified design concept offers scientific reference for precise nutritional support during different developmental stages of children and adolescents.展开更多
Silk fibroin(SF)hydrogels possess excellent biocompatibility and biomimetic properties of the extracellular matrix.Among them,the mild chemical crosslinked SF hydrogels show great application potential in the fields o...Silk fibroin(SF)hydrogels possess excellent biocompatibility and biomimetic properties of the extracellular matrix.Among them,the mild chemical crosslinked SF hydrogels show great application potential in the fields of 3D cell culture and tissue repairing and thus have attracted widespread attention.However,the mobility of hydrophobic chain segments of SF molecules in these chemical crosslinked hydrogels can easily cause the molecules to undergo a self-assembly process from random coil toβ-sheet conformation due to its lower energy state,thus inducing an inevitable conformational transition process.This process further leads to dynamic changes of important material features,such as the hydrogel pore size and mechanical properties,which can probably bring some non-negligible and unknown impacts on cell behaviors and their biomedical applications.In this study,a typical mild crosslinking system composed of horseradish peroxidase and hydrogen peroxide was chosen to prepare SF hydrogels.A feasible protein conformational transition rate controlling strategy based on hydrogel crosslinking density regulation was also proposed.Our results demonstrate that the lower the hydrogel crosslinking density,the faster the conformational transition rate.Subsequently,SF hydrogels with different conformational transition rates were successfully constructed to investigate the impact of the protein conformational transition rate accompanied with initial crosslinking density on the proliferation and chondrogenic differentiation of encapsulated stem cells.Results comprehensively illustrated that the conformational transition process could effectively regulate cell behavior.The hydrogel with an appropriate conformational transition rate obviously promoted the proliferation and chondrogenesis of encapsulated stem cells,while too fast or too slow transition processes slowed down these cell activities.These findings are hopefully to provide valuable guidance for the development and efficient usage of SF hydrogels in the fields of 3D cell culture and tissue engineering.展开更多
文摘Purpose： The purpose of the study was to examine the psychometric properties of the Chinese-translated Behavioral Regulation in Exercise Questionnaire-2 （C-BREQ-2） among Chinese university students from the Mainland and Hong Kong of China. Methods： University students from the Mainland （n = 191） and Hong Kong （n=194） of China participated in this study. Factorial validity, discriminant validity, nomological validity, internal reliability, and measurement invariance across sample of the C-BREQ-2 were examined. Results： Confirmatory factor analysis provided support for the factorial validity of the 18-item, 5-factor structure C-BREQ-2. Examination of the 95% confidence interval of the inter-factor correlations suggested that the C-BREQ-2 assesses related but distinct constructs, which provided support for its discriminant validity. The internal consistency reliability of the C-BREQ-2 was found acceptable. Examination of the pattern of inter-factor correlations between different regulations suggested that a simplex-like pattern was displayed, which provided evidence for the nomological validity of C-BREQ-2. The results from multi-group confirmatory factor analysis suggested that the factor loadings and variances/ covariances of the C-BREQ-2 measurement model were invariant across the Chinese university students in the Mainland and Hong Kong of China. Conclusion： The current study provided further psychometric evidence for the C-BREQ-2, which makes the further application and research of self-determination theory （SDT） based motivation in relation to exercise and physical activity in the Mainland of China context possible.
文摘The preoptic area （POA） is located in the most anterior part of the hypothalamus and is bordered dorsally by the anterior commissure and anteroventrally by the nucleus of the diagonal band of Broca[1]. Accumulating evidence from developmental neurobiology suggests, however, that the POA may be a separate entity from hypothalamus, and may actually be part of the basal telencephalon[2,3]. Both the hypothalamus and POA are highly complex and heterogeneous areas, containing multiple nuclei, each of which has specific fundamental functions for survival. Among these, the POA contains nuclei involved in the regulation of blood osmolality and temperature （the median preoptic nucleus）, sleep （the ventrolateral preoptic and suprachiasmatic nuclei）, ovulation （gonadotropin-releasing hormone neurons scattered mainly in the ventral part of the POA）, male sexual behavior （the medial preoptic nucleus）, and parental behavior （the central part of the medial POA, cMPOA）.
基金supported by the Strategic Priority Research Program of Chinese Academy of Sciences(Grant No.XDB02050400)the National Natural Science Foundation of China(Grant No. #91432111) to Z.Qiu
文摘Methyl-CpG binding protein 2 （MeCP2） has a crucial role in transcriptional regulation and neural development （Ausi6 et al., 2014）. Loss of function mutations of MECP2 in human lead to Rett syndrome （RTT）, a severe neurodevelopmental disorders （Amir et al., 1999）, whereas individuals with the chromosomal duplications containing the MECP2 locus showed severe autism-like symptoms （Ramocki et al., 2009）.
基金supported by the grants from the National Science Foundation of China(Nos. 91132709 and 31130027)
文摘Sugar and protein are the major macronutrients＇ sources, and their balanced intake is important for animal＇s health. It has been observed that animals are able to change food preference in an imbalanced nutritional condition to selectively consume nutrients that are deficient in the body （Dethier, 1976）. Early studies in both Drosophila and mouse have demonstrated that animals exhibit food rejection to imbalanced diets lacking essential amino acids （Hao et al., 2005; Bjordal et al., 2014）. Furthermore, the food preference change upon protein depri- vation has been characterized using a two choice assay in Drosophila （Ribeiro and Dickson, 2010; Vargas et al., 2010）. Different from protein food, sugar is the main energy source, and sugar deficiency severely affects animal survival （Lee et al., 2008）. However, whether animals adopt a strategy of fast food preference switch upon sugar deprivation had not been investigated, and the neural mechanisms underlying this behavior regulation remain poorly understood.
文摘Hyperactivity and tic symptoms in children and adolescents with Attention Deficit Hyperactivity Disorder(ADHD)have a bidirectional association with sleep disorders,while magnesium deficiency is a key nutritional factor exacerbating this vicious cycle.The SLS Magnesium Glycinate Complex Liquid Supplement formula adopts the core logic of“sleep improvement-mood stabilization-behavioral regulation”and enhances the neuromodulatory effects of magnesium ions through multi-component synergy.Tailored to the developmental needs of different stages aged 4-16 years,the formula features a child-friendly dosage design,forming a closed-loop regulation in aiding sleep,reducing tics,and maintaining daytime functioning.A 3-month clinical trial showed that the formula improved sleep efficiency by 15%-23%,reduced tic frequency by 21%-32%,and enhanced emotional stability scores by 24%-30%across all age groups with ADHD,with good safety and tolerability.This study provides a targeted nutritional intervention solution for ADHD,and its age-stratified design concept offers scientific reference for precise nutritional support during different developmental stages of children and adolescents.
基金supported by the International Cooperation Fund of the Science and Technology Commission of Shanghai Municipality(22520711900)the National Natural Science Foundation of China(52273125)+2 种基金the Fundamental Research Funds for the Central Universities(2232024D-01)the National Natural Science Foundation of China(52173031)the Oriental Talent Plan(Leading Talent Program,No.152).
文摘Silk fibroin(SF)hydrogels possess excellent biocompatibility and biomimetic properties of the extracellular matrix.Among them,the mild chemical crosslinked SF hydrogels show great application potential in the fields of 3D cell culture and tissue repairing and thus have attracted widespread attention.However,the mobility of hydrophobic chain segments of SF molecules in these chemical crosslinked hydrogels can easily cause the molecules to undergo a self-assembly process from random coil toβ-sheet conformation due to its lower energy state,thus inducing an inevitable conformational transition process.This process further leads to dynamic changes of important material features,such as the hydrogel pore size and mechanical properties,which can probably bring some non-negligible and unknown impacts on cell behaviors and their biomedical applications.In this study,a typical mild crosslinking system composed of horseradish peroxidase and hydrogen peroxide was chosen to prepare SF hydrogels.A feasible protein conformational transition rate controlling strategy based on hydrogel crosslinking density regulation was also proposed.Our results demonstrate that the lower the hydrogel crosslinking density,the faster the conformational transition rate.Subsequently,SF hydrogels with different conformational transition rates were successfully constructed to investigate the impact of the protein conformational transition rate accompanied with initial crosslinking density on the proliferation and chondrogenic differentiation of encapsulated stem cells.Results comprehensively illustrated that the conformational transition process could effectively regulate cell behavior.The hydrogel with an appropriate conformational transition rate obviously promoted the proliferation and chondrogenesis of encapsulated stem cells,while too fast or too slow transition processes slowed down these cell activities.These findings are hopefully to provide valuable guidance for the development and efficient usage of SF hydrogels in the fields of 3D cell culture and tissue engineering.
