Common bean(Phaseolus vulgaris L.)is a vital source of protein and essential nutrients for human consumption and plays a key role in sustainable agriculture due to its nitrogen-fixing ability(Nadeem et al.,2021).Kidne...Common bean(Phaseolus vulgaris L.)is a vital source of protein and essential nutrients for human consumption and plays a key role in sustainable agriculture due to its nitrogen-fixing ability(Nadeem et al.,2021).Kidney beans,a subcategory of dry common beans,are highly valued for their rich protein,dietary fiber,low fat content,and various trace elements(Garcia-Cordero et al.,2021).Despite the release of several de novo genome assemblies(Goodstein et al.,2012;Schmutz et al.,2014;Vlasova et al.,2016;Cortinovis et al.,2024),existing common bean genomes remain incomplete,particularly in complex regions such as centromeres and telomeres,limiting a comprehensive understanding of the genomic landscape.展开更多
Only one herbicide mode of action (ALS inhibitor) is currently available to Ontario dry bean producers for soil-applied broadleaf weed control. Four field studies were conducted over two years (2014, 2015) to examine ...Only one herbicide mode of action (ALS inhibitor) is currently available to Ontario dry bean producers for soil-applied broadleaf weed control. Four field studies were conducted over two years (2014, 2015) to examine the tolerance of four market classes of dry beans to sulfentrazone (210 and 420 g·ai·ha<sup>-1</sup>) and pyroxasulfone (100 and 200 g·ai·ha<sup>-1</sup>) applied alone and in combination. The registration of these two herbicides would provide Ontario dry bean producers with two additional modes of action for broadleaf weed control. Pyroxasulfone caused up to 23%, 6%, 7% and 10% injury in adzuki, kidney, small red Mexican and white bean, respectively;sulfentrazone caused up to 51%, 12%, 15% and 44% injury and the combination caused up to 90%, 23%, 29% and 62% injury, respectively. Kidney and small red Mexican bean density, height, seed moisture content and yield were not affected. Pyroxasulfone (200 g·ai·ha<sup>-1</sup>) + sulfentrazone (420 g·ai·ha<sup>-1</sup>) reduced adzuki and white bean density, shoot dry weight, height and yield. This study concludes that pyroxasulfone (100 g·ai·ha<sup>-1</sup>) + sulfentrazone (210 g·ai·ha<sup>-1</sup>) applied PRE can be safely used to control weeds in Ontario kidney and small red Mexican bean production.展开更多
Limited information is available on the sensitivity of dry beans to </span><span style="font-family:Verdana;">tribenuron and carfentrazone applied preplant (PP).</span><span style="...Limited information is available on the sensitivity of dry beans to </span><span style="font-family:Verdana;">tribenuron and carfentrazone applied preplant (PP).</span><span style="font-family:""> </span><span style="font-family:""><span style="font-family:Verdana;">Four field trials were conducted at Exeter and Ridgetown, ON, Canada in 2019 and 2020 to determine the toler</span><span style="font-family:Verdana;">ance of azuki, kidney, small red and white beans to glyphosate (1800</span><span style="font-family:Verdana;"> g·ae·</span><span style="font-family:Verdana;">ha</span><sup><span style="font-family:Verdana;">-1</span></sup><span style="font-family:Verdana;">) + tribenuron (15 g</span><span style="font-family:Verdana;">·</span><span style="font-family:Verdana;">ai</span><span style="font-family:Verdana;">·</span><span style="font-family:Verdana;">ha</span><sup><span style="font-family:Verdana;">-1</span></sup><span style="font-family:Verdana;">), glyphosate (1800 </span><a name="_Hlk64540918"></a><span style="font-family:Verdana;">g</span><span style="font-family:Verdana;">·</span><span style="font-family:Verdana;">ae</span><span style="font-family:Verdana;">·</span><span style="font-family:Verdana;">ha</span><sup><span style="font-family:Verdana;">-1</span></sup><span style="font-family:Verdana;">) + carfentrazone (35 </span><span style="font-family:Verdana;">g</span><span style="font-family:Verdana;">·</span><span style="font-family:Verdana;">ai</span><span style="font-family:Verdana;">·</span><span style="font-family:Verdana;">ha</span><sup><span style="font-family:Verdana;">-1</span></sup><span style="font-family:Verdana;">) and glyphosate (1800 g</span><span style="font-family:Verdana;">·</span><span style="font-family:Verdana;">ae</span><span style="font-family:Verdana;">·</span><span style="font-family:Verdana;">ha</span><sup><span style="font-family:Verdana;">-1</span></sup><span style="font-family:Verdana;">) + tribenuron (15 g</span><span style="font-family:Verdana;">·</span><span style="font-family:Verdana;">ai</span><span style="font-family:Verdana;">·</span><span style="font-family:Verdana;">ha</span><sup><span style="font-family:Verdana;">-1</span></sup><span style="font-family:Verdana;">) + car</span><span style="font-family:Verdana;">fentrazone (35 g</span><span style="font-family:Verdana;">·</span><span style="font-family:Verdana;">ai</span><span style="font-family:Verdana;">·</span><span><span style="font-family:Verdana;">ha</span><sup><span style="font-family:Verdana;">-1</span></sup><span style="font-family:Verdana;">) applied PP 1 - 2 days prior to seeding dry beans.</span></span><span style="font-family:Verdana;"> Glyphosate + tribenuron, glyphosate + carfentrazone, and glyphosate + tribenuron + carfentrazone, applied PP, caused 5%, 5% and 9% bean injury at 1 WAE;7%, 6% and 10% bean injury at 2 WAE and 6%, 5% and 8% bean injury at 4 WAE, respectively. At 8 WAE, there was 0, 1% and 4% injury in azuki bean;1%, 2% and 2% injury in kidney bean;3%, 2% and 3% injury in small red bean;and 6%, 3% and 2% injury in white bean with glyphosate + tribenuron, glyphosate + carfentrazone, and glyphosate + tribenuron + carfentrazone applied PP, respectively. The injury was significantly greater with glyphosate + tribenuron in small red and white beans compared to the azuki and kidney beans. There was no difference between injury levels among market classes of dry been with glyphosate + carfentrazone or glyphosate + tribenuron + carfentrazone applied PP. There was no effect of glyphosate + tribenuron, glyphosate + carfentrazone and glyphosate + tribenuron + carfentrazone on dry bean plant stand, maturity and seed yield. However, dry bean biomass was reduced as much as 15% with glyphosate + tribenuron and 13% </span><span style="font-family:Verdana;">with glyphosate + tribenuron + carfentrazone compared to the untreated</span><span style="font-family:Verdana;"> control. Dry bean height was reduced 4% with glyphosate + tribenuron + carfentrazone but was not affected with other treatments. Based on these results, there is potential for using glyphosate plus tribenuron or carfentrazone for preplant weed management in dry bean production.展开更多
Ontario dry bean growers are currently limited to ALS inhibitor herbicides for soilapplied broadleaf weed control;therefore another mode of action is needed. Sulfentrazone is a PPO inhibitor herbicide that has activit...Ontario dry bean growers are currently limited to ALS inhibitor herbicides for soilapplied broadleaf weed control;therefore another mode of action is needed. Sulfentrazone is a PPO inhibitor herbicide that has activity on some annual grass and broadleaf weed species. Four field trials were conducted over two years (2014, 2015) to determine the tolerance of four commonly grown dry bean market classes (adzuki, kidney, small red Mexican and white bean) to PRE applications of sulfentrazone at 140, 210, 280 and 420 g·ai·ha<sup>-1</sup>. Crop injury, plant height, plant density, shoot biomass, seed moisture content and yield were examined. Sulfentrazone (420 g·ai·ha<sup>-1</sup>) caused up to 74%, 22%, 30%, and 57% injury in adzuki, kidney, small red Mexican and white bean, respectively. Plant density, height and yield were not reduced for kidney or small red Mexican bean. Sulfentrazone (420 g·ai·ha<sup>-1</sup>) reduced white bean plant density, height and yield by 28%, 29% and 29%, respectively;and reduced adzuki bean plant density, height and yield by 51%, 34% and 57%, respectively. Overall, kidney and small red Mexican bean were the most tolerant to sulfentrazone, followed by white bean, and then adzuki. This study determined sulfentrazone applied PRE is safe for Ontario kidney bean and small red Mexican bean crops.展开更多
Synthetic fertilizers are widely used to address the urgent challenge of ensuring food supplies for a growing world population in the context of climate change. However, their industrial production and use in agricult...Synthetic fertilizers are widely used to address the urgent challenge of ensuring food supplies for a growing world population in the context of climate change. However, their industrial production and use in agriculture have a negative impact on the environment and consequently on human health. While chemical fertilizers may not have to be abandoned in agricultural production systems, limiting their use could help to make agriculture sustainable and resilient to climate change. In Senegal, the level of mineral fertilizers used in market gardening has become alarming in the Niayes area. As a result, microbial biotechnologies have been promoted for biofertilizer production of common bean (Phaseolus vulgaris L.) cultivation. Rhizobial inoculums have thus been used to reduce the rate of chemical nitrogen fertilizers being applied in cropping systems. Several investigations in the laboratory, on experimental stations and in the field have shown a possibility of a significant reduction in the use of nitrogen fertilizers in common bean production. Conventional mineral fertilization use can be reduced from over 120 kg N/ha to 20 kg N/ha. This contributes both to a very significant reduction in the application rate with the same level of yield and to an improvement in the standard of living. In addition, the environmental impact of using chemical fertilizers can be mitigated. This study is a contribution to the promotion of biofertilizers adoption in agricultural systems.展开更多
Côte d’Ivoire has been the world’s leading producer of cocoa beans for several decades. Apart from this production performance, the quality of the beans, which are mainly exported to the major chocolate-making ...Côte d’Ivoire has been the world’s leading producer of cocoa beans for several decades. Apart from this production performance, the quality of the beans, which are mainly exported to the major chocolate-making countries, presents a quality problem to the point of suffering a discount on the international market. One of these quality problems is the content of ochratoxin A, a mycotoxin produced by fungi. Finally, to verify the level of contamination in beans produced in Côte d’Ivoire, a study was carried out. It consisted of collecting information on fermentation and drying times (The two major post-harvest operations) and collecting beans, which were analyzed by electrophoresis using the High Performance Liquid Chromatography (HPLC) method. The results obtained show ochratoxin A contents of between 0.05 µg/kg and 0.17 µg/kg. The general level of contamination is therefore very low and below the tolerable limit which is 2 µg/kg. In addition, the correlative study between the fermentation and drying times of the beans revealed no significant influence (p < 0.01) of the duration of these operations on the level of ochratoxin A contamination. Major contamination can occur after post-harvest activities carried out by producers. This is certainly due to the development of fungi responsible for the production of ochratoxin A during the period of storage and marketing of cocoa beans in conditions of high humidity in storage enclosures. Producers need to be made more aware of the need to ensure that cocoa beans are properly dried and stored in dry areas to avoid moisture build-up, which is a source of mould growth and ochratoxin A production.展开更多
Encapsulation and protection of hesperidin(HES)in mung bean protein isolate(MPI)-dextran(DX)conjugatestabilized nanoemulsions(MDC NEs)were investigated in this study.The degree of grafting of MDC prepared by a dry-hea...Encapsulation and protection of hesperidin(HES)in mung bean protein isolate(MPI)-dextran(DX)conjugatestabilized nanoemulsions(MDC NEs)were investigated in this study.The degree of grafting of MDC prepared by a dry-heating method reached 39.70%±0.01% under the optimal conditions of MPI/DX mass ratio 1:2.3,reaction temperature 58.8℃,and reaction time 4 d.Moreover,the analyses of Fourier infrared spectroscopy,intrinsic fluorescence spectroscopy,surface hydrophobicity,and thermal stability further confirmed the covalent grafting of dextran onto MPI molecules.When encapsulated in MDC NEs at 80 MPa for three times by highpressure homogenization,the encapsulation efficiency and loading capacity of HES were 63.62%±0.01%and 0.40±0.00 g/g,respectively.The encapsulated HES exhibited higher antioxidant activity and stronger light and storage stability than the free HES.Additionally,the incorporation of HES inhibited the formation of lipid peroxides in the nanoemulsions.The findings suggest that glycosylation combined with high-pressure homogenization is an effective strategy for enhancing the stability of MPI-based emulsions and improving their encapsulation of HES.This study provides a promising approach for the development of innovative food and beverage products based on MPI emulsions or new materials for encapsulating fat-soluble bioactive compounds.展开更多
Ozone(O_(3))pollution has elevated in China,threatening plants and crop production.Ethylenediurea(EDU)is a chemical alleviating O_(3)-induced phytotoxicities.This study aimed at revealing fluctuating O_(3) exposures e...Ozone(O_(3))pollution has elevated in China,threatening plants and crop production.Ethylenediurea(EDU)is a chemical alleviating O_(3)-induced phytotoxicities.This study aimed at revealing fluctuating O_(3) exposures effects on Youxian No 3(Phaseolus vulgaris)and Sukui No 4(Vigna angularis),two widely grown Chinese bean cultivars,and EDU role in mediating these effects.Plants were periodically treated with EDU(400 mg/L)or water and subjected to charcoal-filtered air(CF)or non-filtered ambient air enriched with an additional targeted O_(3) concentration of 40 ppb(NF40).with subsequent ambient or NF40 exposures.A 10-day exposure to NF40 increased photosynthetic rate(A)while decreasing the leaf intercellular CO_(2) concentration(Ci),but this effect was absent after moving plants to ambient air for two weeks.Moving previously CF-exposed plants to ambient air for two weeks also increased A,which was not linked with Ci but more related to stomatal conductance(gs).Following two one-week and two-week sequential exposures of all plants to NF40,with an intermediate exposure to ambient air,elevated O_(3) reduced chlorophylls(SPAD),A,gs,Ci,and transpiration and decoupled A-gs response.More O_(3) effectswere observed in plants treatedwithNF40 during each O_(3)-treatment cycle,compared to those exposed to CF during the first cycle.The former plants exhibited significantly decreased biomass and water content of leaves and stems but increased flowers biomass and water content.Some of the effects were cultivardependent,with Youxian showing more apparent trade-offs between vegetative growth and reproduction.EDU alleviated various negative O_(3) effects.展开更多
[Objectives]This study was conducted to quickly qualitatively and quantitatively analyze the residues of 10 plant growth regulators(PGRs)in bean sprouts.[Methods]Using bean sprouts as the test material,a high-performa...[Objectives]This study was conducted to quickly qualitatively and quantitatively analyze the residues of 10 plant growth regulators(PGRs)in bean sprouts.[Methods]Using bean sprouts as the test material,a high-performance liquid chromatography-tandem mass spectrometry(HPLC-MS/MS)method was established to determine the residual levels of 10 PGRs in bean sprouts.[Results]Under optimized conditions,the retention time of the 10 PGRs ranged from 6.45 to 11.43 min.When the mass concentration ranged from 0.005 to 0.05μg/ml,all PGRs exhibited good linearity,with correlation coefficients(r)≥0.9991.The limits of detection(LODs,S/N=3)were in the range of 0.30-0.92μg/kg,and the limits of quantification(LOQs)were in the range of 0.50-2.10μg/kg.The average recovery values at three concentration levels ranged from 80%to 105.8%,with relative standard deviations(RSD s,n=6)of 2.8%-7.5%.[Conclusions]This method is simple and accurate,and provides technical reference for food safety monitoring.展开更多
Objective:To explore the effects of acupoint catgut embedding combined with auricular point pressing with beans on symptom management self-efficacy and quality of life in patients with nonalcoholic steatohepatitis(NAS...Objective:To explore the effects of acupoint catgut embedding combined with auricular point pressing with beans on symptom management self-efficacy and quality of life in patients with nonalcoholic steatohepatitis(NASH)of liver depression and spleen deficiency type.Methods:Sixty patients with NASH of liver depression and spleen deficiency type admitted to our hospital from January 2021 to December 2023 were selected and divided into an acupoint catgut embedding group(n=30)and a combined group(n=30)using the envelope lottery method.The acupoint catgut embedding group received acupoint catgut embedding intervention,while the combined group received auricular point pressing with beans on the basis of the acupoint catgut embedding group.The two groups were compared in terms of TCM syndrome scores,symptom management self-efficacy[Chronic Disease Self-Efficacy Scale(CDSES)],and quality of life[Chronic Liver Disease Questionnaire(CLDQ)].Results:After intervention,the combined group had lower TCM syndrome scores for both primary and secondary symptoms compared to the acupoint catgut embedding group(P<0.05).The combined group also had higher scores in all dimensions and total score of the CDSES compared to the acupoint catgut embedding group(P<0.05).Similarly,the combined group had higher scores in all dimensions and total score of the CLDQ compared to the acupoint catgut embedding group(P<0.05).Conclusion:Acupoint catgut embedding combined with auricular point pressing with beans can effectively improve TCM symptoms,enhance symptom management self-efficacy,and improve quality of life in patients with NASH of liver depression and spleen deficiency type.展开更多
The high-efficiency planting mode for five crops a year of fresh edible ＂faba bean/spring maize＋soybean-autumn maize/autumn soybean＂ was introduced, and its yield and economic benefits were compared with the planti...The high-efficiency planting mode for five crops a year of fresh edible ＂faba bean/spring maize＋soybean-autumn maize/autumn soybean＂ was introduced, and its yield and economic benefits were compared with the planting mode of three crops a year of ＂faba bean-spring maize/red bean＂. The results showed that the planting method for fresh edible ＂faba bean/spring maize＋soybean-autumn maize/autumn soybean＂ was much easier to operate with the input-output ratio of about185.6%, and its yield and economic benefits were 2.09 and 1.83 times of that of the planting mode for three crops a year, significantly improving the agricultural yield and income of farmers. In addition, the cropping index of the planting mode for fresh edible fresh edible ＂faba bean/spring maize ＋soybean-autumn maize/autumn soybean＂ reached up to 350%, and planting faba bean once a year and soybean twice a year could make the biological fixation amount of nitrogen increase 350-450kg/m^2, which equaled to up to 700 kg/m^2 of urea, showing significant ecological and social benefits. Based on the comparison results, the high-yield culture techniques of the planting mode of fresh edible ＂faba bean/spring maize＋soybean-autumn maize/autumn soybean＂ were summarized.展开更多
Soybean is one of the most important sources of edible oil and proteins in the world. However, it suffers from many kinds of fungal diseases which is a major limiting factor in soybean production. The fungal disease c...Soybean is one of the most important sources of edible oil and proteins in the world. However, it suffers from many kinds of fungal diseases which is a major limiting factor in soybean production. The fungal disease can be effectively controlled by breeding plant cultivars with genetic transformation. In this study, the resistance to Phytophthora sojae of five bivalent transgenic soybean lines was identified using the hypocotyls inoculation technique. The lines were the T2 of the transgenic soybean which were transformed with kidney bean chitinase gene and barley ribosome inactivating protein gene, and were positive by Southern Blot analysis. The resistance difference was studied through comparing the death percentage of transgenic soybean with the control. The results showed that four lines were more resistant to P sojae, whereas other one had no significant difference in comparison with the control. These transgenic soybean lines with enhanced resistance to P sojae will be useful in soybean resistance breeding.展开更多
In coffee breeding practice,two quantitative traits,namely cherry and green bean characters are the important phenotypic selection index.The synchronous of cherry maturation,size,weight and shape of green bean are des...In coffee breeding practice,two quantitative traits,namely cherry and green bean characters are the important phenotypic selection index.The synchronous of cherry maturation,size,weight and shape of green bean are desirable traits for the future breeding.In order to increase the breeding efficiency,a set of quantitative trait loci(QTLs)analysis controlling these traits was carried out.The QTL analysis was performanced in cross pollinated population of Coffea canephora using single nucleotide polymorphisms(SNPs)and simple sequence repeats(SSRs)markers.Totally 12 QTLs determined desirable cherry and green bean traits were identified on 9 linkage groups(LG),where these QTLs mapped forming clusters in 11 different chromosomal regions.The desirable traits which can be detected by their QTLs are synchronous of cherry maturation(LG B and LG G),cherry size(LG A,LG F and LG G),bean shape(LG B,LG D and LG J),bean weight(LG H),bean size(LG A and LG E)and cherry-bean size(LG K)The gene action of these QTLs was dominance or an interaction of alleles(epistasis between alleles at the studied locus)effect.The yielded linkage map and the detected QTL can provide a tool for further genetic analysis of two traits and be potential for maker-assisted selection in C.canephora breeding.展开更多
基金supported by the National Natural Science Foundation of China(32241045,32241046,32241038)the Major Special Science and Technology Projects in Shanxi Province(202101140601027)+3 种基金Shanxi Provincial Agricultural Key Technologies Breakthrough Project(NYGG01)Doctoral Research Starting Project at Shanxi Agricultural University(2024BQ77)the National Key Research and Development Program of China(2023YFD1202705/2023YFD120270503,2023YFD1202703/2023YFD1202703-4)Shanxi HouJi Laboratory Self-proposed Research Project(202304010930003/202304010930003-03).
文摘Common bean(Phaseolus vulgaris L.)is a vital source of protein and essential nutrients for human consumption and plays a key role in sustainable agriculture due to its nitrogen-fixing ability(Nadeem et al.,2021).Kidney beans,a subcategory of dry common beans,are highly valued for their rich protein,dietary fiber,low fat content,and various trace elements(Garcia-Cordero et al.,2021).Despite the release of several de novo genome assemblies(Goodstein et al.,2012;Schmutz et al.,2014;Vlasova et al.,2016;Cortinovis et al.,2024),existing common bean genomes remain incomplete,particularly in complex regions such as centromeres and telomeres,limiting a comprehensive understanding of the genomic landscape.
文摘Only one herbicide mode of action (ALS inhibitor) is currently available to Ontario dry bean producers for soil-applied broadleaf weed control. Four field studies were conducted over two years (2014, 2015) to examine the tolerance of four market classes of dry beans to sulfentrazone (210 and 420 g·ai·ha<sup>-1</sup>) and pyroxasulfone (100 and 200 g·ai·ha<sup>-1</sup>) applied alone and in combination. The registration of these two herbicides would provide Ontario dry bean producers with two additional modes of action for broadleaf weed control. Pyroxasulfone caused up to 23%, 6%, 7% and 10% injury in adzuki, kidney, small red Mexican and white bean, respectively;sulfentrazone caused up to 51%, 12%, 15% and 44% injury and the combination caused up to 90%, 23%, 29% and 62% injury, respectively. Kidney and small red Mexican bean density, height, seed moisture content and yield were not affected. Pyroxasulfone (200 g·ai·ha<sup>-1</sup>) + sulfentrazone (420 g·ai·ha<sup>-1</sup>) reduced adzuki and white bean density, shoot dry weight, height and yield. This study concludes that pyroxasulfone (100 g·ai·ha<sup>-1</sup>) + sulfentrazone (210 g·ai·ha<sup>-1</sup>) applied PRE can be safely used to control weeds in Ontario kidney and small red Mexican bean production.
文摘Limited information is available on the sensitivity of dry beans to </span><span style="font-family:Verdana;">tribenuron and carfentrazone applied preplant (PP).</span><span style="font-family:""> </span><span style="font-family:""><span style="font-family:Verdana;">Four field trials were conducted at Exeter and Ridgetown, ON, Canada in 2019 and 2020 to determine the toler</span><span style="font-family:Verdana;">ance of azuki, kidney, small red and white beans to glyphosate (1800</span><span style="font-family:Verdana;"> g·ae·</span><span style="font-family:Verdana;">ha</span><sup><span style="font-family:Verdana;">-1</span></sup><span style="font-family:Verdana;">) + tribenuron (15 g</span><span style="font-family:Verdana;">·</span><span style="font-family:Verdana;">ai</span><span style="font-family:Verdana;">·</span><span style="font-family:Verdana;">ha</span><sup><span style="font-family:Verdana;">-1</span></sup><span style="font-family:Verdana;">), glyphosate (1800 </span><a name="_Hlk64540918"></a><span style="font-family:Verdana;">g</span><span style="font-family:Verdana;">·</span><span style="font-family:Verdana;">ae</span><span style="font-family:Verdana;">·</span><span style="font-family:Verdana;">ha</span><sup><span style="font-family:Verdana;">-1</span></sup><span style="font-family:Verdana;">) + carfentrazone (35 </span><span style="font-family:Verdana;">g</span><span style="font-family:Verdana;">·</span><span style="font-family:Verdana;">ai</span><span style="font-family:Verdana;">·</span><span style="font-family:Verdana;">ha</span><sup><span style="font-family:Verdana;">-1</span></sup><span style="font-family:Verdana;">) and glyphosate (1800 g</span><span style="font-family:Verdana;">·</span><span style="font-family:Verdana;">ae</span><span style="font-family:Verdana;">·</span><span style="font-family:Verdana;">ha</span><sup><span style="font-family:Verdana;">-1</span></sup><span style="font-family:Verdana;">) + tribenuron (15 g</span><span style="font-family:Verdana;">·</span><span style="font-family:Verdana;">ai</span><span style="font-family:Verdana;">·</span><span style="font-family:Verdana;">ha</span><sup><span style="font-family:Verdana;">-1</span></sup><span style="font-family:Verdana;">) + car</span><span style="font-family:Verdana;">fentrazone (35 g</span><span style="font-family:Verdana;">·</span><span style="font-family:Verdana;">ai</span><span style="font-family:Verdana;">·</span><span><span style="font-family:Verdana;">ha</span><sup><span style="font-family:Verdana;">-1</span></sup><span style="font-family:Verdana;">) applied PP 1 - 2 days prior to seeding dry beans.</span></span><span style="font-family:Verdana;"> Glyphosate + tribenuron, glyphosate + carfentrazone, and glyphosate + tribenuron + carfentrazone, applied PP, caused 5%, 5% and 9% bean injury at 1 WAE;7%, 6% and 10% bean injury at 2 WAE and 6%, 5% and 8% bean injury at 4 WAE, respectively. At 8 WAE, there was 0, 1% and 4% injury in azuki bean;1%, 2% and 2% injury in kidney bean;3%, 2% and 3% injury in small red bean;and 6%, 3% and 2% injury in white bean with glyphosate + tribenuron, glyphosate + carfentrazone, and glyphosate + tribenuron + carfentrazone applied PP, respectively. The injury was significantly greater with glyphosate + tribenuron in small red and white beans compared to the azuki and kidney beans. There was no difference between injury levels among market classes of dry been with glyphosate + carfentrazone or glyphosate + tribenuron + carfentrazone applied PP. There was no effect of glyphosate + tribenuron, glyphosate + carfentrazone and glyphosate + tribenuron + carfentrazone on dry bean plant stand, maturity and seed yield. However, dry bean biomass was reduced as much as 15% with glyphosate + tribenuron and 13% </span><span style="font-family:Verdana;">with glyphosate + tribenuron + carfentrazone compared to the untreated</span><span style="font-family:Verdana;"> control. Dry bean height was reduced 4% with glyphosate + tribenuron + carfentrazone but was not affected with other treatments. Based on these results, there is potential for using glyphosate plus tribenuron or carfentrazone for preplant weed management in dry bean production.
文摘Ontario dry bean growers are currently limited to ALS inhibitor herbicides for soilapplied broadleaf weed control;therefore another mode of action is needed. Sulfentrazone is a PPO inhibitor herbicide that has activity on some annual grass and broadleaf weed species. Four field trials were conducted over two years (2014, 2015) to determine the tolerance of four commonly grown dry bean market classes (adzuki, kidney, small red Mexican and white bean) to PRE applications of sulfentrazone at 140, 210, 280 and 420 g·ai·ha<sup>-1</sup>. Crop injury, plant height, plant density, shoot biomass, seed moisture content and yield were examined. Sulfentrazone (420 g·ai·ha<sup>-1</sup>) caused up to 74%, 22%, 30%, and 57% injury in adzuki, kidney, small red Mexican and white bean, respectively. Plant density, height and yield were not reduced for kidney or small red Mexican bean. Sulfentrazone (420 g·ai·ha<sup>-1</sup>) reduced white bean plant density, height and yield by 28%, 29% and 29%, respectively;and reduced adzuki bean plant density, height and yield by 51%, 34% and 57%, respectively. Overall, kidney and small red Mexican bean were the most tolerant to sulfentrazone, followed by white bean, and then adzuki. This study determined sulfentrazone applied PRE is safe for Ontario kidney bean and small red Mexican bean crops.
文摘Synthetic fertilizers are widely used to address the urgent challenge of ensuring food supplies for a growing world population in the context of climate change. However, their industrial production and use in agriculture have a negative impact on the environment and consequently on human health. While chemical fertilizers may not have to be abandoned in agricultural production systems, limiting their use could help to make agriculture sustainable and resilient to climate change. In Senegal, the level of mineral fertilizers used in market gardening has become alarming in the Niayes area. As a result, microbial biotechnologies have been promoted for biofertilizer production of common bean (Phaseolus vulgaris L.) cultivation. Rhizobial inoculums have thus been used to reduce the rate of chemical nitrogen fertilizers being applied in cropping systems. Several investigations in the laboratory, on experimental stations and in the field have shown a possibility of a significant reduction in the use of nitrogen fertilizers in common bean production. Conventional mineral fertilization use can be reduced from over 120 kg N/ha to 20 kg N/ha. This contributes both to a very significant reduction in the application rate with the same level of yield and to an improvement in the standard of living. In addition, the environmental impact of using chemical fertilizers can be mitigated. This study is a contribution to the promotion of biofertilizers adoption in agricultural systems.
文摘Côte d’Ivoire has been the world’s leading producer of cocoa beans for several decades. Apart from this production performance, the quality of the beans, which are mainly exported to the major chocolate-making countries, presents a quality problem to the point of suffering a discount on the international market. One of these quality problems is the content of ochratoxin A, a mycotoxin produced by fungi. Finally, to verify the level of contamination in beans produced in Côte d’Ivoire, a study was carried out. It consisted of collecting information on fermentation and drying times (The two major post-harvest operations) and collecting beans, which were analyzed by electrophoresis using the High Performance Liquid Chromatography (HPLC) method. The results obtained show ochratoxin A contents of between 0.05 µg/kg and 0.17 µg/kg. The general level of contamination is therefore very low and below the tolerable limit which is 2 µg/kg. In addition, the correlative study between the fermentation and drying times of the beans revealed no significant influence (p < 0.01) of the duration of these operations on the level of ochratoxin A contamination. Major contamination can occur after post-harvest activities carried out by producers. This is certainly due to the development of fungi responsible for the production of ochratoxin A during the period of storage and marketing of cocoa beans in conditions of high humidity in storage enclosures. Producers need to be made more aware of the need to ensure that cocoa beans are properly dried and stored in dry areas to avoid moisture build-up, which is a source of mould growth and ochratoxin A production.
基金financially supported by the National Natural Science Foundation of China(Grant No.32101981)the cooperation project between Ya’an city and Sichuan Agricultural University(23ZDF0003)。
文摘Encapsulation and protection of hesperidin(HES)in mung bean protein isolate(MPI)-dextran(DX)conjugatestabilized nanoemulsions(MDC NEs)were investigated in this study.The degree of grafting of MDC prepared by a dry-heating method reached 39.70%±0.01% under the optimal conditions of MPI/DX mass ratio 1:2.3,reaction temperature 58.8℃,and reaction time 4 d.Moreover,the analyses of Fourier infrared spectroscopy,intrinsic fluorescence spectroscopy,surface hydrophobicity,and thermal stability further confirmed the covalent grafting of dextran onto MPI molecules.When encapsulated in MDC NEs at 80 MPa for three times by highpressure homogenization,the encapsulation efficiency and loading capacity of HES were 63.62%±0.01%and 0.40±0.00 g/g,respectively.The encapsulated HES exhibited higher antioxidant activity and stronger light and storage stability than the free HES.Additionally,the incorporation of HES inhibited the formation of lipid peroxides in the nanoemulsions.The findings suggest that glycosylation combined with high-pressure homogenization is an effective strategy for enhancing the stability of MPI-based emulsions and improving their encapsulation of HES.This study provides a promising approach for the development of innovative food and beverage products based on MPI emulsions or new materials for encapsulating fat-soluble bioactive compounds.
基金supported by the National Natural Science Foundation of China(No.31950410547 and 42107270).
文摘Ozone(O_(3))pollution has elevated in China,threatening plants and crop production.Ethylenediurea(EDU)is a chemical alleviating O_(3)-induced phytotoxicities.This study aimed at revealing fluctuating O_(3) exposures effects on Youxian No 3(Phaseolus vulgaris)and Sukui No 4(Vigna angularis),two widely grown Chinese bean cultivars,and EDU role in mediating these effects.Plants were periodically treated with EDU(400 mg/L)or water and subjected to charcoal-filtered air(CF)or non-filtered ambient air enriched with an additional targeted O_(3) concentration of 40 ppb(NF40).with subsequent ambient or NF40 exposures.A 10-day exposure to NF40 increased photosynthetic rate(A)while decreasing the leaf intercellular CO_(2) concentration(Ci),but this effect was absent after moving plants to ambient air for two weeks.Moving previously CF-exposed plants to ambient air for two weeks also increased A,which was not linked with Ci but more related to stomatal conductance(gs).Following two one-week and two-week sequential exposures of all plants to NF40,with an intermediate exposure to ambient air,elevated O_(3) reduced chlorophylls(SPAD),A,gs,Ci,and transpiration and decoupled A-gs response.More O_(3) effectswere observed in plants treatedwithNF40 during each O_(3)-treatment cycle,compared to those exposed to CF during the first cycle.The former plants exhibited significantly decreased biomass and water content of leaves and stems but increased flowers biomass and water content.Some of the effects were cultivardependent,with Youxian showing more apparent trade-offs between vegetative growth and reproduction.EDU alleviated various negative O_(3) effects.
基金Supported by Outstanding Talent Development Program of Hebei ProvinceTangshan Talent Funding Project(A202202005).
文摘[Objectives]This study was conducted to quickly qualitatively and quantitatively analyze the residues of 10 plant growth regulators(PGRs)in bean sprouts.[Methods]Using bean sprouts as the test material,a high-performance liquid chromatography-tandem mass spectrometry(HPLC-MS/MS)method was established to determine the residual levels of 10 PGRs in bean sprouts.[Results]Under optimized conditions,the retention time of the 10 PGRs ranged from 6.45 to 11.43 min.When the mass concentration ranged from 0.005 to 0.05μg/ml,all PGRs exhibited good linearity,with correlation coefficients(r)≥0.9991.The limits of detection(LODs,S/N=3)were in the range of 0.30-0.92μg/kg,and the limits of quantification(LOQs)were in the range of 0.50-2.10μg/kg.The average recovery values at three concentration levels ranged from 80%to 105.8%,with relative standard deviations(RSD s,n=6)of 2.8%-7.5%.[Conclusions]This method is simple and accurate,and provides technical reference for food safety monitoring.
文摘Objective:To explore the effects of acupoint catgut embedding combined with auricular point pressing with beans on symptom management self-efficacy and quality of life in patients with nonalcoholic steatohepatitis(NASH)of liver depression and spleen deficiency type.Methods:Sixty patients with NASH of liver depression and spleen deficiency type admitted to our hospital from January 2021 to December 2023 were selected and divided into an acupoint catgut embedding group(n=30)and a combined group(n=30)using the envelope lottery method.The acupoint catgut embedding group received acupoint catgut embedding intervention,while the combined group received auricular point pressing with beans on the basis of the acupoint catgut embedding group.The two groups were compared in terms of TCM syndrome scores,symptom management self-efficacy[Chronic Disease Self-Efficacy Scale(CDSES)],and quality of life[Chronic Liver Disease Questionnaire(CLDQ)].Results:After intervention,the combined group had lower TCM syndrome scores for both primary and secondary symptoms compared to the acupoint catgut embedding group(P<0.05).The combined group also had higher scores in all dimensions and total score of the CDSES compared to the acupoint catgut embedding group(P<0.05).Similarly,the combined group had higher scores in all dimensions and total score of the CLDQ compared to the acupoint catgut embedding group(P<0.05).Conclusion:Acupoint catgut embedding combined with auricular point pressing with beans can effectively improve TCM symptoms,enhance symptom management self-efficacy,and improve quality of life in patients with NASH of liver depression and spleen deficiency type.
基金Supported by the China Agriculture Research System(CARS-09)the Technological Innovation and Industrialization Project for the Rural Area of Nantong City,Jiangsu Province(H12014012)~~
文摘The high-efficiency planting mode for five crops a year of fresh edible ＂faba bean/spring maize＋soybean-autumn maize/autumn soybean＂ was introduced, and its yield and economic benefits were compared with the planting mode of three crops a year of ＂faba bean-spring maize/red bean＂. The results showed that the planting method for fresh edible ＂faba bean/spring maize＋soybean-autumn maize/autumn soybean＂ was much easier to operate with the input-output ratio of about185.6%, and its yield and economic benefits were 2.09 and 1.83 times of that of the planting mode for three crops a year, significantly improving the agricultural yield and income of farmers. In addition, the cropping index of the planting mode for fresh edible fresh edible ＂faba bean/spring maize ＋soybean-autumn maize/autumn soybean＂ reached up to 350%, and planting faba bean once a year and soybean twice a year could make the biological fixation amount of nitrogen increase 350-450kg/m^2, which equaled to up to 700 kg/m^2 of urea, showing significant ecological and social benefits. Based on the comparison results, the high-yield culture techniques of the planting mode of fresh edible ＂faba bean/spring maize＋soybean-autumn maize/autumn soybean＂ were summarized.
基金Supported by the National Items of Research and Industrial Development of Transgenic Plants(J99-B-013)
文摘Soybean is one of the most important sources of edible oil and proteins in the world. However, it suffers from many kinds of fungal diseases which is a major limiting factor in soybean production. The fungal disease can be effectively controlled by breeding plant cultivars with genetic transformation. In this study, the resistance to Phytophthora sojae of five bivalent transgenic soybean lines was identified using the hypocotyls inoculation technique. The lines were the T2 of the transgenic soybean which were transformed with kidney bean chitinase gene and barley ribosome inactivating protein gene, and were positive by Southern Blot analysis. The resistance difference was studied through comparing the death percentage of transgenic soybean with the control. The results showed that four lines were more resistant to P sojae, whereas other one had no significant difference in comparison with the control. These transgenic soybean lines with enhanced resistance to P sojae will be useful in soybean resistance breeding.
文摘In coffee breeding practice,two quantitative traits,namely cherry and green bean characters are the important phenotypic selection index.The synchronous of cherry maturation,size,weight and shape of green bean are desirable traits for the future breeding.In order to increase the breeding efficiency,a set of quantitative trait loci(QTLs)analysis controlling these traits was carried out.The QTL analysis was performanced in cross pollinated population of Coffea canephora using single nucleotide polymorphisms(SNPs)and simple sequence repeats(SSRs)markers.Totally 12 QTLs determined desirable cherry and green bean traits were identified on 9 linkage groups(LG),where these QTLs mapped forming clusters in 11 different chromosomal regions.The desirable traits which can be detected by their QTLs are synchronous of cherry maturation(LG B and LG G),cherry size(LG A,LG F and LG G),bean shape(LG B,LG D and LG J),bean weight(LG H),bean size(LG A and LG E)and cherry-bean size(LG K)The gene action of these QTLs was dominance or an interaction of alleles(epistasis between alleles at the studied locus)effect.The yielded linkage map and the detected QTL can provide a tool for further genetic analysis of two traits and be potential for maker-assisted selection in C.canephora breeding.
