Cetaceans include the largest animals ever to have lived onearth and are uniparous(producing a single calf at each birth)across the infraorder.However,instances of multiple fetuseshave been observed naturally among un...Cetaceans include the largest animals ever to have lived onearth and are uniparous(producing a single calf at each birth)across the infraorder.However,instances of multiple fetuseshave been observed naturally among uniparous mammals,including cetaceans.Despite this,there is no known documented case of twins in cetaceans successfully carried to termin the wild(Perrin and Donovan 1984),and if such casesexist,they would be diffcult to detect.展开更多
Crossed beak is a complex mode of inheritance with prevalence ranging from 0.2 to 7.4% in at least 12 chicken strains worldwide.To reveal the intrinsic factors causing crossed beaks,genes expression patterns in bilate...Crossed beak is a complex mode of inheritance with prevalence ranging from 0.2 to 7.4% in at least 12 chicken strains worldwide.To reveal the intrinsic factors causing crossed beaks,genes expression patterns in bilateral mandibular condyle between affected and normal birds were characterized by RNA sequencing analysis in the present studies.Crossed beak was induced by short length of unilateral mandibular ramus,and a total of 110differentially expressed genes were up-or down-regulated in the affected(short)mandibular condyle side as compared to the normal side.Carbonic anhydrase 2(CA2)and Carbonic anhydrase 13(CA13)were enriched in the carbonate dehydratase activity,and high-expressed in mandibular condyle and osteoblasts(P<0.05).However,both were low-expressed in short mandibular condyle side of affected birds(P<0.05).The carbonate dehydratase inhibitor experiments confirmed that there is positive association between the calcification and carbonic anhydrase isoenzymes.Quantitative analysis with cetylpyridinium chloride showed a decrease in calcification when the cells were transfected with an anti-CA13 shRNA.Our research suggested that CA2 and CA13 are down-calcified in shortside mandibular condyle,and caused mandibular ramus to grow slowly.CA2 and CA13 have the critical role in crossed beaks by regulating calcification of mandibular condyle.展开更多
Stable isotopes(δ^(13)C and δ^(15)N) have been widely used to track cephalopod habitat, migration and trophic structure. In this study, we analyzed the δ^(13)C and δ^(15)N values in 245 Dosidicus gigas beaks colle...Stable isotopes(δ^(13)C and δ^(15)N) have been widely used to track cephalopod habitat, migration and trophic structure. In this study, we analyzed the δ^(13)C and δ^(15)N values in 245 Dosidicus gigas beaks collected during 2009–2010 and in 2013 off Peruvian Exclusive Economic Zone(EEZ) waters. High individual variation in beak stable isotopes was shown with values ranging from-19.4‰ to-15.8‰ for δ^(13)C and from 5.0‰ to 15.1‰ for δ^(15)N. A generalized additive model(GAM) showed that latitude, mantle length and distance to shelf break significantly described the isotope variation with deviance ranging from 16.6% in δ^(13)C to 36.3% in δ^(15)N. Large variability in beak δ^(13)C values for a given size and sampling station indicated that D. gigas off the Peruvian EEZ waters migrate in different ways when they occupy a large range of habitats for their ontogeny. Low baseline δ^(15)N values suggested that spatial changes in diet trophic level may be the main determinative factor in beak δ^(15)N variation. We conclude that high variability in beak δ^(15)N values for a given set of explanatory variables indicated that D. gigas is an opportunistic predator with highly diverse dietary habitats. This study further develops our knowledge of the life history of D. gigas in such a highly dynamic region.展开更多
The migration route of oceanic squid provides critical information for us to understand their spatial and temporal variations.Mark-recapture and electronic tags tend to be problematic during processing.Cephalopod hard...The migration route of oceanic squid provides critical information for us to understand their spatial and temporal variations.Mark-recapture and electronic tags tend to be problematic during processing.Cephalopod hard structures such as the beak,containing abundant ecological information with stable morphology and statolithlike sequences of growth increments,may provide information for studying spatio-temporal distribution.In this study,we developed a method,which is based on elemental concentration of beaks at different ontogenetic stages and sampling locations,to reconstruct the squid migration route.We applied this method to Ommastrephes bartramii in the North Pacific Ocean.Nine trace elements were detected in the rostrum sagittal sections(RSS)of the beak using laser ablation inductively coupled plasma mass spectrometry(LA-ICP-MS).For those elements,significant differences were found between the different ontogenetic stages for phosphorus(P),copper(Cu)and zinc(Zn).Sodium(Na),P and Zn were chosen as indicators of sea surface temperature(SST)and a regression model was estimated.The high probability of occurrence in a particular area represented the possible optimal squid location based on a Bayesian model.A reconstructed migration route in this study,combining all the locations at different ontogenetic stages,was consistent with that hypothesized in previous studies.This study demonstrates that the beak can provide useful information for identifying the migration routes of oceanic squid.展开更多
The cephalopod beak is a vital hard structure with a stable configuration and has been widely used for the identification of cephalopod species. This study was conducted to determine the best standardization method fo...The cephalopod beak is a vital hard structure with a stable configuration and has been widely used for the identification of cephalopod species. This study was conducted to determine the best standardization method for identifying different species by measuring 12 morphological variables of the beaks of Illex argentinus, Ommastrephes bartramii, and Dosidicus gigas that were collected by Chinese jigging vessels. To remove the effects of size, these morphometric variables were standardized using three methods. The average ratios of the upper beak morphological variables and upper crest length of O. bartramii and D. gigas were found to be greater than those of I. argentinus. However, for lower beaks, only the average of LRL(lower rostrum length)/LCL(lower crest length), LRW(lower rostrum width)/LCL, and LLWL(lower lateral wall length)/LCL of O. bartramii and D. gigas were greater than those of I. argentinus. The ratios of beak morphological variables and crest length were found to be all significantly different among the three species(P < 0.001). Among the three standardization methods, the correct classification rate of stepwise discriminant analysis(SDA) was the highest using the ratios of beak morphological variables and crest length. Compared with hood length, the correct classification rate was slightly higher when using beak variables standardized by crest length using an allometric model. The correct classification rate of the lower beak was also found to be greater than that of the upper beak. This study indicates that the ratios of beak morphological variables to crest length could be used for interspecies and intraspecies identification. Meanwhile, the lower beak variables were found to be more effective than upper beak variables in classifying beaks found in the stomachs of predators.展开更多
The mitre squid(U roteuthis chinensis) and the swordtip squid( U. edulis) are Indo-Pacific cephalopod species that are abundant in the western Pacific Ocean. They are currently exploited in the East and South China Se...The mitre squid(U roteuthis chinensis) and the swordtip squid( U. edulis) are Indo-Pacific cephalopod species that are abundant in the western Pacific Ocean. They are currently exploited in the East and South China Seas and make up a significant portion of the Chinese neritic squid catch. Beaks, the feeding organs of squid, are important for individual size and biomass estimation because of their high resistance to degradation in predator stomachs and consistent dimensions. In this study, 104 U. chinensis and 143 U. edulis individuals were sampled from northern South China Sea with mantle length from 70 to 260 mm and 96 to 284 mm, respectively. The results indicated that morphological beak values were greater for U. edulis, compared to U. chinensis, for upper hood length(UHL), upper crest length(UCL), upper lateral wall length(ULWL), lower crest length(LCL), and lower lateral wall length(LLWL). According to principal component analysis, UHL/ML, UCL/ML, ULWL/ML, LCL/ML, LLWL/ML and LWL/ML could represent the characteristics of beaks for U. chinensis, while UHL/ML, UCL/ML, ULWL/ML, LHL/ML, LCL/ML and LLWL/ML could represent it for U. edulis. According to Akaike's information criterion(AIC) values, a power function was the most suitable model for U. chinensis, while a linear function was the most suitable model for U. edulis. The beak variable-mantle length ratio(beak variable/mantle length) declined with the increasing of mantle length and declined sharply at the early stage of growth in both beaks and species. The ratio changed quickly after achieving the mantle length of 140 mm for U. chinensis, while the ratio changed quickly after 170 mm for U. edulis. Beaks in both species experienced sharper changes through maturity stage I to II than other maturity stages. This study gives us basic beak morphology information for U. chinensis and U. edulis in the East and South China Seas. Geometric morphological methods combined with dietary analysis should be used in the future.展开更多
Beak of cephalopod is an important hard tissue. Understanding the morphology of beak can yield critical infor- mation on the role of cephalopods in the ecosystem. The south patagonic stock of the Argentine shortfin sq...Beak of cephalopod is an important hard tissue. Understanding the morphology of beak can yield critical infor- mation on the role of cephalopods in the ecosystem. The south patagonic stock of the Argentine shortfin squid, Illex argentinus, is not only one of the most important fishing targets, but also one of the most important species in the marine eco-system of the southwest Atlantic. A total of 430 samples ofL argentinus, including 229 females 103-346mm in mantle length （ML） and 201 males 140-298mm in ML, were collected from the area off the Exclusive Economic Zone of Argentinean waters by Chinese squid jigging vessels during February to May 2007. The morphology of their beaks was evaluated. The relationships between beak morphological variables and ML differed significantly among males and females. They could be best described by loga- rithmic functions for females and linear functions for males except for upper wing length （UWL） and lower rostrum length （LRL）, which followed exponential functions in their relationships with ML. The results showed the sexual dimorphism in the relationship between ML and beak morphology for the south patagonic stock ofL argentinus. However, no significant differ- ence was found between males and females in the relationships of beak morphological variables （except for UWL） versus body weight （BW）, suggesting that the relationship between beak morphological variables and BW can be used for estimating the biomass consumed by their predators.展开更多
The Humboldt squid Dosidicus gigas has a short life span, and environmental variability plays a significant role in regulating its population dynamics and distribution. An analysis of 1 096 samples of D. gigas collect...The Humboldt squid Dosidicus gigas has a short life span, and environmental variability plays a significant role in regulating its population dynamics and distribution. An analysis of 1 096 samples of D. gigas collected by the Chinese commercial fishing vessels during 2013, 2014, and 2016 off the Peruvian Exclusive Economic Zone, was conducted to evaluate the impacts of El Nino events on the somatic condition of D. gigas. This study indicates that the slopes of all beak variables in relation to mantle length (ML) for females were greater than those of males during 2013, 2014, and 2016, and slopes of the upper crest length and the lower rostrum length significantly differed between females and males in 2013 (P<0.05). Variation in the slopes for beak variables among years was studied;no significant difference was observed (ANCOVA, P>0.05). The Fulton's condition coefficients (K) of females and males in 2013 and 2014 were significantly greater than those in 2016 (P<0.01). The K values of females were greater than those of males in 2013, 2014, and 2016, and K values significantly differed between females and males in 2013. In normal years, the chlorophyll a (Chl a) concentration showed an N-shaped variability from January to December. However, in the El Nino period, it tended to weaken the upwelling coupled with warm and low Chl a concentration waters. We suggest that the poor somatic condition of D. gigas during the El Nino year was resulted from the low Chl a concentration in the waters, and the abundance of D. gigas would decrease due to the unfavourable environment and the lack of prey items in the El Nino year.展开更多
We quantified theδ^13C andδ^15N values in the lower beaks of Humboldt squid,Dosidicus gigas,collected from international waters off Costa Rica,Ecuador,Peru and Chile by Chinese squid jigging vessels during 2009,2010...We quantified theδ^13C andδ^15N values in the lower beaks of Humboldt squid,Dosidicus gigas,collected from international waters off Costa Rica,Ecuador,Peru and Chile by Chinese squid jigging vessels during 2009,2010 and 2013.There was a significant difference in the isotopic values among regions with the lowest value off Ecuador and the highest off Chile,which were interpreted as a function of trophic effects as well as baseline values.However,constant trophic level of D.gigas across its geographic range showed that spatial variation in the baseline of primary production is the main driver responsible for the observed geographic isotope variability.Inter-regional difference and intra-regional convergence of isotope values indicated squid off Costa Rica,Ecuador and Chile belong to different geographically segregated populations,which were previously proved by integrated population identifying method.In contrast,the higher variations inδ^13C andδ^15N values in a given size group suggest the squid off Peru move and forage in different places.Moreover,potential population exchange could be responsible for the overlap of the isotope values between the squid off Peru and off Chile.On the whole,the spatial difference in isotopic values of Humboldt squid beaks improves our understanding of potential geographic population connectivity and movement.展开更多
Cephalopods play key roles in global marine ecosystems as both predators and preys.Regressive estimation of original size and weight of cephalopod from beak measurements is a powerful tool of interrogating the feeding...Cephalopods play key roles in global marine ecosystems as both predators and preys.Regressive estimation of original size and weight of cephalopod from beak measurements is a powerful tool of interrogating the feeding ecology of predators at higher trophic levels.In this study,regressive relationships among beak measurements and body length and weight were determined for an octopus species(Octopus variabilis),an important endemic cephalopod species in the northwest Pacific Ocean.A total of 193 individuals(63 males and 130 females) were collected at a monthly interval from Jiaozhou Bay,China.Regressive relationships among 6 beak measurements(upper hood length,UHL;upper crest length,UCL;lower hood length,LHL;lower crest length,LCL;and upper and lower beak weights) and mantle length(ML),total length(TL) and body weight(W) were determined.Results showed that the relationships between beak size and TL and beak size and ML were linearly regressive,while those between beak size and W fitted a power function model.LHL and UCL were the most useful measurements for estimating the size and biomass of O.variabilis.The relationships among beak measurements and body length(either ML or TL) were not significantly different between two sexes;while those among several beak measurements(UHL,LHL and LBW) and body weight(W) were sexually different.Since male individuals of this species have a slightly greater body weight distribution than female individuals,the body weight was not an appropriate measurement for estimating size and biomass,especially when the sex of individuals in the stomachs of predators was unknown.These relationships provided essential information for future use in size and biomass estimation of O.variabilis,as well as the estimation of predator/prey size ratios in the diet of top predators.展开更多
Beak and feather disease virus(BFDV) is an infectious agent responsible for feather degeneration and beak deformation in birds. In March 2017, an epidemic of psittacine beak and feather disease(PBFD) struck a farm in ...Beak and feather disease virus(BFDV) is an infectious agent responsible for feather degeneration and beak deformation in birds. In March 2017, an epidemic of psittacine beak and feather disease(PBFD) struck a farm in Fuzhou in the Fujian Province of southeast China, resulting in the death of 51 parrots. In this study, the disease was diagnosed and the pathogen was identified by PCR and whole genome sequencing. A distinct BFDV strain was identified and named as the FZ strain.This BFDV strain caused severe disease symptoms and pathological changes characteristic of typical PBFD in parrots, for example, loss of feathers and deformities of the beak and claws, and severe pathological changes in multiple organs of the infected birds. Phylogenetic analysis showed that the FZ strain was more closely related to the strain circulating in New Caledonia than the strains previously reported in China. Nucleotide homology between the FZ strain and other 43 strains of BFDV ranged from 80.0% to 92.0%. Blind passage experiment showed that this strain had limited replication capability in SPF Chicken Embryos and DF-1 Cells. Furthermore, the capsid(Cap) gene of this FZ strain was cloned into the p GEX-4 T-1 expression vector to prepare the polyclonal anti-Cap antibody. Western blotting analysis using the anti-Cap antibody further confirmed that the diseased parrots were infected with BFDV. In this study, a PBFD and its pathogen was identified for the first time in Fujian Province of China, suggesting that future surveillance of BFDV should be performed.展开更多
Beak trimming was a part of routine husbandry for poultry industry,which was the most efficient and economic way to reduce the incidence of cannibalism.However,the controversy about young chicken trimming still exits ...Beak trimming was a part of routine husbandry for poultry industry,which was the most efficient and economic way to reduce the incidence of cannibalism.However,the controversy about young chicken trimming still exits and it was drawing more concern recently along with the popular of animal behavior and welfare theory.To evaluate the influence of beak trimming on young chicken spleen development and find the key regulators of the spleen development,in this study,the electron microscope technique,flow cytometry and immunohistochemical techniques were used to investigate the effects of beak trimming stress on the spleen development.The result of electronic microscope showed that significant stress response could be found in chicken spleen cells after beak trimming.Cell volume became larger,chromatin of cell nuclear agglutinated conglobation,cytoplasm formed more vacuole,and mitochondria emerged vacuolization phenomenon in splenic lymphocyte after beak trimming.From the flow cytometry,the lymphocyte of spleen in the control group and experimental group were mainly in G1 phase,and the remaining small amount was in S and M phases.Of interest,the number of the lymphocyte which are in G1 phase within immune organ in the experimental group were more than that in the control group,but the number of cells in S and M phases were less than that in the control group.And beak trimming decreased the lymphocytes proliferation index of spleen.Inmmuohistochemical result showed that the beak trimming stress did not affect the expression sites of two apoptosis-related genes,Bcl-2 and Bax,in the spleen.But the expression level of Bcl-2 decreased while Bax increased in chicken immune organs after beak trimming.Furthermore,the beak trimming increased spleen cell apoptosis with a time-dependant style.Our findings may make a contribution to the active precaution of stress response.展开更多
Observations were made on one eye from a Longman’s beaked whale Indopacetus pacificus, which was probably one of the least known extant cetaceans. The whale died shortly after swimming aground on the coast in the Nis...Observations were made on one eye from a Longman’s beaked whale Indopacetus pacificus, which was probably one of the least known extant cetaceans. The whale died shortly after swimming aground on the coast in the Nishikata Beach, Sendai-shi, Kagoshima-ken Prefecture, Japan, on July 26, 2002. It was a mature female with body length 6.45 m. This paper documented the basic structural characteristics of her visual organ of the whale in order to better understand this animal.展开更多
The static tests of nine traditional and bird beak square hollow structure(SHS) T-joints with different β values and connection types under axial compression at brace end were carried out. Experimental test schemes, ...The static tests of nine traditional and bird beak square hollow structure(SHS) T-joints with different β values and connection types under axial compression at brace end were carried out. Experimental test schemes, failure modes of specimens, jack load-vertical displacement curves, jack load-deformation of chord and strain intensity distribution curves of joints were presented. The effects of β and connection types on axial compression property of joints were studied. The results show that the ultimate axial compression capacity of common bird beak SHS T-joints and diamond bird beak SHS T-joints is larger than that of traditional SHS T-joint specimens with big values of β. The ultimate axial compression capacity of diamond bird beak SHS T-joints is larger than that of common bird beak SHS T-joints. As β increases, the increase of the ultimate axial compression capacity of diamond bird beak SHS T-joints over that of common bird beak joints grows. The ultimate axial compression capacity and the initial axial stiffness of all kinds of joints increase as β increases, and the initial axial stiffness of the diamond bird beak SHS T-joints is the largest. The ductilities of common bird beak and diamond bird beak SHS T-joints increase as β increases, but the ductility of the traditional SHS T-joints decreases as β increases.展开更多
Psittacine beak and feather disease virus (PBFDV) and avian polyomavirus (APV) are the most common viral diseases in psittacine birds, both affecting feathers and physical appearance of birds. Between 2005 and 2009, a...Psittacine beak and feather disease virus (PBFDV) and avian polyomavirus (APV) are the most common viral diseases in psittacine birds, both affecting feathers and physical appearance of birds. Between 2005 and 2009, a total of 269 samples were collected from birds presented at veterinary clinics, shelters and rescue centers of wildlife in Costa Rica. They belonged to 19 species of psittacine birds. The most representative species in the sample were Ara macao (157), Ara ambigua (37), Amazona autumnalis (24), Amazon ochrocephala (21) and Ara ararauna (8). A prevalence of 19.7% (53/269) for PBFDV and 4.8% (13/269) for APV was determined using Polymerase Chain Reaction (PCR). In 3.3% (9/269) of the birds mixed infections were detected. Statistical analysis determined that psittacines living in shelters and rescue centers had a greater risk to be positive to PBFDV and APV than birds that were presented at veterinary clinics, while only for PBFDV it was determined, that it is more likely to detect it in feathers than in blood. Finally, birds infected with PBFDV had 6.24 times more probability to become infected with APV, than non-infected birds. This is the first report of prevalence of PBFDV and APV in captive psittacines from Costa Rica.展开更多
Beaked whales represent around 25%of known extant cetacean species,yet they are the least known of all marine mammals.Identification of many Mesoplodon species has relied on examination of a few stranded individuals.P...Beaked whales represent around 25%of known extant cetacean species,yet they are the least known of all marine mammals.Identification of many Mesoplodon species has relied on examination of a few stranded individuals.Particularly,the ginkgo-toothed beaked whale(Mesoplodon ginkgodens)and Deraniyagala’s beaked whale(Mesoplodon hotaula)are among the least-known of beaked whale species,without confirmed sightings of living individuals to date.We present a sighting of 3 free-ranging individuals of M.ginkgodens/hotaula whale from a dedicated marine mammal vessel survey carried out in the South China Sea in April and May 2019.Photographic data(301 photographs)from the sighting were compared to photos of fresh stranded ginkgo-toothed beaked whale and Deraniyagala’s beaked whale from both historical and unpublished records.We found that free-ranging M.ginkgodens and M.hotaula individuals can be easily distinguished from other Mesoplodon species due to differences in melon and gape shapes and coloration patterns.However,accurate at-sea differentiation of M.ginkgodens and M.hotaula may not be possible due to high similarity in both coloration and scarring patterns.In addition to our photo-identification data,we collected what we believe to be the first preliminary descriptions of surfacing behavior and diving patterns of one of these species.Finally,the presence of scars possibly caused by fishing gear or marine litter raises concerns about anthropogenic impacts and conservation of these poorly known species.展开更多
文摘Cetaceans include the largest animals ever to have lived onearth and are uniparous(producing a single calf at each birth)across the infraorder.However,instances of multiple fetuseshave been observed naturally among uniparous mammals,including cetaceans.Despite this,there is no known documented case of twins in cetaceans successfully carried to termin the wild(Perrin and Donovan 1984),and if such casesexist,they would be diffcult to detect.
基金supported by the Beijing Featured Livestock and Poultry Genetic Resources Preservation Project,China(202203310002)China Agriculture Research System of MOF and MARA(CARS40)+1 种基金the Agricultural Science and Technology Innovation Program of Chinese Academy of Agricultural Sciences(ASTIPIAS04)the Central Guidance on Local Science and Technology Development Fund of Hebei Province,China(236Z6602G)。
文摘Crossed beak is a complex mode of inheritance with prevalence ranging from 0.2 to 7.4% in at least 12 chicken strains worldwide.To reveal the intrinsic factors causing crossed beaks,genes expression patterns in bilateral mandibular condyle between affected and normal birds were characterized by RNA sequencing analysis in the present studies.Crossed beak was induced by short length of unilateral mandibular ramus,and a total of 110differentially expressed genes were up-or down-regulated in the affected(short)mandibular condyle side as compared to the normal side.Carbonic anhydrase 2(CA2)and Carbonic anhydrase 13(CA13)were enriched in the carbonate dehydratase activity,and high-expressed in mandibular condyle and osteoblasts(P<0.05).However,both were low-expressed in short mandibular condyle side of affected birds(P<0.05).The carbonate dehydratase inhibitor experiments confirmed that there is positive association between the calcification and carbonic anhydrase isoenzymes.Quantitative analysis with cetylpyridinium chloride showed a decrease in calcification when the cells were transfected with an anti-CA13 shRNA.Our research suggested that CA2 and CA13 are down-calcified in shortside mandibular condyle,and caused mandibular ramus to grow slowly.CA2 and CA13 have the critical role in crossed beaks by regulating calcification of mandibular condyle.
基金sponsored by the Shanghai Pujiang Program (No. 18PJ1404100)the National Natural Science Foundation of China (Nos. 41306127 and 41276156)
文摘Stable isotopes(δ^(13)C and δ^(15)N) have been widely used to track cephalopod habitat, migration and trophic structure. In this study, we analyzed the δ^(13)C and δ^(15)N values in 245 Dosidicus gigas beaks collected during 2009–2010 and in 2013 off Peruvian Exclusive Economic Zone(EEZ) waters. High individual variation in beak stable isotopes was shown with values ranging from-19.4‰ to-15.8‰ for δ^(13)C and from 5.0‰ to 15.1‰ for δ^(15)N. A generalized additive model(GAM) showed that latitude, mantle length and distance to shelf break significantly described the isotope variation with deviance ranging from 16.6% in δ^(13)C to 36.3% in δ^(15)N. Large variability in beak δ^(13)C values for a given size and sampling station indicated that D. gigas off the Peruvian EEZ waters migrate in different ways when they occupy a large range of habitats for their ontogeny. Low baseline δ^(15)N values suggested that spatial changes in diet trophic level may be the main determinative factor in beak δ^(15)N variation. We conclude that high variability in beak δ^(15)N values for a given set of explanatory variables indicated that D. gigas is an opportunistic predator with highly diverse dietary habitats. This study further develops our knowledge of the life history of D. gigas in such a highly dynamic region.
基金The National Natural Science Foundation of China under contract No.NSFC4147129the China Postdoctoral Science Foundation under contract No.2017M610277+1 种基金the Key Laboratory of Sustainable Exploitation of Oceanic Fisheries Resources(Shanghai Ocean University),Ministry of Education under contract No.A1-0203-00-2009-6the Fund of Key Laboratory of Open-Sea Fishery Development,Ministry of Agriculture,China under contract LOF 2018-02
文摘The migration route of oceanic squid provides critical information for us to understand their spatial and temporal variations.Mark-recapture and electronic tags tend to be problematic during processing.Cephalopod hard structures such as the beak,containing abundant ecological information with stable morphology and statolithlike sequences of growth increments,may provide information for studying spatio-temporal distribution.In this study,we developed a method,which is based on elemental concentration of beaks at different ontogenetic stages and sampling locations,to reconstruct the squid migration route.We applied this method to Ommastrephes bartramii in the North Pacific Ocean.Nine trace elements were detected in the rostrum sagittal sections(RSS)of the beak using laser ablation inductively coupled plasma mass spectrometry(LA-ICP-MS).For those elements,significant differences were found between the different ontogenetic stages for phosphorus(P),copper(Cu)and zinc(Zn).Sodium(Na),P and Zn were chosen as indicators of sea surface temperature(SST)and a regression model was estimated.The high probability of occurrence in a particular area represented the possible optimal squid location based on a Bayesian model.A reconstructed migration route in this study,combining all the locations at different ontogenetic stages,was consistent with that hypothesized in previous studies.This study demonstrates that the beak can provide useful information for identifying the migration routes of oceanic squid.
基金supported by the National Natural Science Foundation of China(Nos.41306127 and 41276156)the National Science Foundation of Shanghai(No.13ZR1419700)+3 种基金the Innovation Program of Shanghai Municipal Education Commission(No.13YZ091)the Shanghai Leading Academic Discipline Project(Fisheries Discipline)supported by Shanghai Ocean University(SHOU)International Center for Marine StudiesShanghai Visiting 1000 Talent Program
文摘The cephalopod beak is a vital hard structure with a stable configuration and has been widely used for the identification of cephalopod species. This study was conducted to determine the best standardization method for identifying different species by measuring 12 morphological variables of the beaks of Illex argentinus, Ommastrephes bartramii, and Dosidicus gigas that were collected by Chinese jigging vessels. To remove the effects of size, these morphometric variables were standardized using three methods. The average ratios of the upper beak morphological variables and upper crest length of O. bartramii and D. gigas were found to be greater than those of I. argentinus. However, for lower beaks, only the average of LRL(lower rostrum length)/LCL(lower crest length), LRW(lower rostrum width)/LCL, and LLWL(lower lateral wall length)/LCL of O. bartramii and D. gigas were greater than those of I. argentinus. The ratios of beak morphological variables and crest length were found to be all significantly different among the three species(P < 0.001). Among the three standardization methods, the correct classification rate of stepwise discriminant analysis(SDA) was the highest using the ratios of beak morphological variables and crest length. Compared with hood length, the correct classification rate was slightly higher when using beak variables standardized by crest length using an allometric model. The correct classification rate of the lower beak was also found to be greater than that of the upper beak. This study indicates that the ratios of beak morphological variables to crest length could be used for interspecies and intraspecies identification. Meanwhile, the lower beak variables were found to be more effective than upper beak variables in classifying beaks found in the stomachs of predators.
基金Supported by the National Natural Science Foundation of China(Nos.41306127,41276156)supported by SHOU International Center for Marine Studies and Shanghai 1000 Talent Program
文摘The mitre squid(U roteuthis chinensis) and the swordtip squid( U. edulis) are Indo-Pacific cephalopod species that are abundant in the western Pacific Ocean. They are currently exploited in the East and South China Seas and make up a significant portion of the Chinese neritic squid catch. Beaks, the feeding organs of squid, are important for individual size and biomass estimation because of their high resistance to degradation in predator stomachs and consistent dimensions. In this study, 104 U. chinensis and 143 U. edulis individuals were sampled from northern South China Sea with mantle length from 70 to 260 mm and 96 to 284 mm, respectively. The results indicated that morphological beak values were greater for U. edulis, compared to U. chinensis, for upper hood length(UHL), upper crest length(UCL), upper lateral wall length(ULWL), lower crest length(LCL), and lower lateral wall length(LLWL). According to principal component analysis, UHL/ML, UCL/ML, ULWL/ML, LCL/ML, LLWL/ML and LWL/ML could represent the characteristics of beaks for U. chinensis, while UHL/ML, UCL/ML, ULWL/ML, LHL/ML, LCL/ML and LLWL/ML could represent it for U. edulis. According to Akaike's information criterion(AIC) values, a power function was the most suitable model for U. chinensis, while a linear function was the most suitable model for U. edulis. The beak variable-mantle length ratio(beak variable/mantle length) declined with the increasing of mantle length and declined sharply at the early stage of growth in both beaks and species. The ratio changed quickly after achieving the mantle length of 140 mm for U. chinensis, while the ratio changed quickly after 170 mm for U. edulis. Beaks in both species experienced sharper changes through maturity stage I to II than other maturity stages. This study gives us basic beak morphology information for U. chinensis and U. edulis in the East and South China Seas. Geometric morphological methods combined with dietary analysis should be used in the future.
基金funded by National Science Foundation of China (NSFC41276156)sponsored by Program of Shanghai Subject Chief Scientist (10XD-1402000)+3 种基金Foundation of Doctorate Programs of Ministry of Education of China (20093104110002)Shanghai Leading Academic Discipline Project (Fisheries Discipline)Y. Chen’s involvement in the project was supported by the Shanghai Dongfang Scholar ProgramSupports from Xinshiji No. 52 for the scientific survey are gratefully acknowledged
文摘Beak of cephalopod is an important hard tissue. Understanding the morphology of beak can yield critical infor- mation on the role of cephalopods in the ecosystem. The south patagonic stock of the Argentine shortfin squid, Illex argentinus, is not only one of the most important fishing targets, but also one of the most important species in the marine eco-system of the southwest Atlantic. A total of 430 samples ofL argentinus, including 229 females 103-346mm in mantle length （ML） and 201 males 140-298mm in ML, were collected from the area off the Exclusive Economic Zone of Argentinean waters by Chinese squid jigging vessels during February to May 2007. The morphology of their beaks was evaluated. The relationships between beak morphological variables and ML differed significantly among males and females. They could be best described by loga- rithmic functions for females and linear functions for males except for upper wing length （UWL） and lower rostrum length （LRL）, which followed exponential functions in their relationships with ML. The results showed the sexual dimorphism in the relationship between ML and beak morphology for the south patagonic stock ofL argentinus. However, no significant differ- ence was found between males and females in the relationships of beak morphological variables （except for UWL） versus body weight （BW）, suggesting that the relationship between beak morphological variables and BW can be used for estimating the biomass consumed by their predators.
基金Supported by the National Natural Science Foundation of China(Nos.NSFC41306127,NSFC41276156)the National Science Foundation of Shanghai(No.13ZR1419700)+2 种基金the Innovation Program of Shanghai Municipal Education Commission(No.13YZ091)the Operational Application Project of Satellite Ocean Remote Sensing(No.201701004)the Shanghai Leading Academic Discipline Project(Fisheries Discipline)
文摘The Humboldt squid Dosidicus gigas has a short life span, and environmental variability plays a significant role in regulating its population dynamics and distribution. An analysis of 1 096 samples of D. gigas collected by the Chinese commercial fishing vessels during 2013, 2014, and 2016 off the Peruvian Exclusive Economic Zone, was conducted to evaluate the impacts of El Nino events on the somatic condition of D. gigas. This study indicates that the slopes of all beak variables in relation to mantle length (ML) for females were greater than those of males during 2013, 2014, and 2016, and slopes of the upper crest length and the lower rostrum length significantly differed between females and males in 2013 (P<0.05). Variation in the slopes for beak variables among years was studied;no significant difference was observed (ANCOVA, P>0.05). The Fulton's condition coefficients (K) of females and males in 2013 and 2014 were significantly greater than those in 2016 (P<0.01). The K values of females were greater than those of males in 2013, 2014, and 2016, and K values significantly differed between females and males in 2013. In normal years, the chlorophyll a (Chl a) concentration showed an N-shaped variability from January to December. However, in the El Nino period, it tended to weaken the upwelling coupled with warm and low Chl a concentration waters. We suggest that the poor somatic condition of D. gigas during the El Nino year was resulted from the low Chl a concentration in the waters, and the abundance of D. gigas would decrease due to the unfavourable environment and the lack of prey items in the El Nino year.
基金The Shanghai Pujiang Program under contract No.18PJ1404100the Program for Professor of Special Appointment(Eastern Scholar)at Shanghai Institutions of Higher Learning under contract No.0810000243the National Natural Science Foundation of China under contract Nos 41306127 and 41276156
文摘We quantified theδ^13C andδ^15N values in the lower beaks of Humboldt squid,Dosidicus gigas,collected from international waters off Costa Rica,Ecuador,Peru and Chile by Chinese squid jigging vessels during 2009,2010 and 2013.There was a significant difference in the isotopic values among regions with the lowest value off Ecuador and the highest off Chile,which were interpreted as a function of trophic effects as well as baseline values.However,constant trophic level of D.gigas across its geographic range showed that spatial variation in the baseline of primary production is the main driver responsible for the observed geographic isotope variability.Inter-regional difference and intra-regional convergence of isotope values indicated squid off Costa Rica,Ecuador and Chile belong to different geographically segregated populations,which were previously proved by integrated population identifying method.In contrast,the higher variations inδ^13C andδ^15N values in a given size group suggest the squid off Peru move and forage in different places.Moreover,potential population exchange could be responsible for the overlap of the isotope values between the squid off Peru and off Chile.On the whole,the spatial difference in isotopic values of Humboldt squid beaks improves our understanding of potential geographic population connectivity and movement.
基金funded by The National Natural Science Foundation of China(41006083)The Shandong Provincial Natural Science Foundation,China(ZR2010DQ026)+1 种基金The Fundamental Research Funds for the Central Universities(201022001,201262004)The Specialized Research Program for Marine Public Welfare Industry from the State Oceanic Administration,P.R.China(200805066)
文摘Cephalopods play key roles in global marine ecosystems as both predators and preys.Regressive estimation of original size and weight of cephalopod from beak measurements is a powerful tool of interrogating the feeding ecology of predators at higher trophic levels.In this study,regressive relationships among beak measurements and body length and weight were determined for an octopus species(Octopus variabilis),an important endemic cephalopod species in the northwest Pacific Ocean.A total of 193 individuals(63 males and 130 females) were collected at a monthly interval from Jiaozhou Bay,China.Regressive relationships among 6 beak measurements(upper hood length,UHL;upper crest length,UCL;lower hood length,LHL;lower crest length,LCL;and upper and lower beak weights) and mantle length(ML),total length(TL) and body weight(W) were determined.Results showed that the relationships between beak size and TL and beak size and ML were linearly regressive,while those between beak size and W fitted a power function model.LHL and UCL were the most useful measurements for estimating the size and biomass of O.variabilis.The relationships among beak measurements and body length(either ML or TL) were not significantly different between two sexes;while those among several beak measurements(UHL,LHL and LBW) and body weight(W) were sexually different.Since male individuals of this species have a slightly greater body weight distribution than female individuals,the body weight was not an appropriate measurement for estimating size and biomass,especially when the sex of individuals in the stomachs of predators was unknown.These relationships provided essential information for future use in size and biomass estimation of O.variabilis,as well as the estimation of predator/prey size ratios in the diet of top predators.
基金the National Key Research and Development Program of China(2018YFD0500100,2016YFD0500205)Discipline Development Grant from College of Animal Sciences FAFU(2018DK003)+3 种基金Natural Science Foundation of China(31602046)National Basic Research Program(973)of China(2015CB910502)Program for Fujian University Outstanding Youth Scientific Research,Science and Technology Innovation Special Foundation of Fujian Agriculture and Forestry University(KFA17229A)Collaborative Innovation Center of Animal Health and Food Safety Application Technology in Fujian,Fujian Vocational College of Agriculture(Kla17H01A).
文摘Beak and feather disease virus(BFDV) is an infectious agent responsible for feather degeneration and beak deformation in birds. In March 2017, an epidemic of psittacine beak and feather disease(PBFD) struck a farm in Fuzhou in the Fujian Province of southeast China, resulting in the death of 51 parrots. In this study, the disease was diagnosed and the pathogen was identified by PCR and whole genome sequencing. A distinct BFDV strain was identified and named as the FZ strain.This BFDV strain caused severe disease symptoms and pathological changes characteristic of typical PBFD in parrots, for example, loss of feathers and deformities of the beak and claws, and severe pathological changes in multiple organs of the infected birds. Phylogenetic analysis showed that the FZ strain was more closely related to the strain circulating in New Caledonia than the strains previously reported in China. Nucleotide homology between the FZ strain and other 43 strains of BFDV ranged from 80.0% to 92.0%. Blind passage experiment showed that this strain had limited replication capability in SPF Chicken Embryos and DF-1 Cells. Furthermore, the capsid(Cap) gene of this FZ strain was cloned into the p GEX-4 T-1 expression vector to prepare the polyclonal anti-Cap antibody. Western blotting analysis using the anti-Cap antibody further confirmed that the diseased parrots were infected with BFDV. In this study, a PBFD and its pathogen was identified for the first time in Fujian Province of China, suggesting that future surveillance of BFDV should be performed.
基金supported by the National Natural Science Foundation of China(31072023)the National Agricultural Science and Technology Achievements Transformation Foundation of China(2009GB2D000218)as well as the Earmarked Fund for Modern Agro-Industry Technology Research System,China(CARS-41-K04)
文摘Beak trimming was a part of routine husbandry for poultry industry,which was the most efficient and economic way to reduce the incidence of cannibalism.However,the controversy about young chicken trimming still exits and it was drawing more concern recently along with the popular of animal behavior and welfare theory.To evaluate the influence of beak trimming on young chicken spleen development and find the key regulators of the spleen development,in this study,the electron microscope technique,flow cytometry and immunohistochemical techniques were used to investigate the effects of beak trimming stress on the spleen development.The result of electronic microscope showed that significant stress response could be found in chicken spleen cells after beak trimming.Cell volume became larger,chromatin of cell nuclear agglutinated conglobation,cytoplasm formed more vacuole,and mitochondria emerged vacuolization phenomenon in splenic lymphocyte after beak trimming.From the flow cytometry,the lymphocyte of spleen in the control group and experimental group were mainly in G1 phase,and the remaining small amount was in S and M phases.Of interest,the number of the lymphocyte which are in G1 phase within immune organ in the experimental group were more than that in the control group,but the number of cells in S and M phases were less than that in the control group.And beak trimming decreased the lymphocytes proliferation index of spleen.Inmmuohistochemical result showed that the beak trimming stress did not affect the expression sites of two apoptosis-related genes,Bcl-2 and Bax,in the spleen.But the expression level of Bcl-2 decreased while Bax increased in chicken immune organs after beak trimming.Furthermore,the beak trimming increased spleen cell apoptosis with a time-dependant style.Our findings may make a contribution to the active precaution of stress response.
基金the National Science Museum,Tokyo 169-0073,JapanNNSF of China (No.40376042)Natural Science Foundation of Shandong Province (No.Y2007D75)
文摘Observations were made on one eye from a Longman’s beaked whale Indopacetus pacificus, which was probably one of the least known extant cetaceans. The whale died shortly after swimming aground on the coast in the Nishikata Beach, Sendai-shi, Kagoshima-ken Prefecture, Japan, on July 26, 2002. It was a mature female with body length 6.45 m. This paper documented the basic structural characteristics of her visual organ of the whale in order to better understand this animal.
基金Projects(51278209,51478047)supported by the National Natural Science Foundation of ChinaProject(2014FJ-NCET-ZR03)supported by the Program for New Century Excellent Talents in Fujian Provincial Universities,China+1 种基金Project(JA13005)supported by the Incubation Program for Excellent Young Science and Technology Talents in Fujian Provincial Universities,ChinaProject(ZQN-PY110)supported by the Young and Middle-aged Academic Staff of Huaqiao University,China
文摘The static tests of nine traditional and bird beak square hollow structure(SHS) T-joints with different β values and connection types under axial compression at brace end were carried out. Experimental test schemes, failure modes of specimens, jack load-vertical displacement curves, jack load-deformation of chord and strain intensity distribution curves of joints were presented. The effects of β and connection types on axial compression property of joints were studied. The results show that the ultimate axial compression capacity of common bird beak SHS T-joints and diamond bird beak SHS T-joints is larger than that of traditional SHS T-joint specimens with big values of β. The ultimate axial compression capacity of diamond bird beak SHS T-joints is larger than that of common bird beak SHS T-joints. As β increases, the increase of the ultimate axial compression capacity of diamond bird beak SHS T-joints over that of common bird beak joints grows. The ultimate axial compression capacity and the initial axial stiffness of all kinds of joints increase as β increases, and the initial axial stiffness of the diamond bird beak SHS T-joints is the largest. The ductilities of common bird beak and diamond bird beak SHS T-joints increase as β increases, but the ductility of the traditional SHS T-joints decreases as β increases.
基金the German Service of Academic Exchange(DAAD)for the scholarship awarded to Jessica Sheleby-Elias.
文摘Psittacine beak and feather disease virus (PBFDV) and avian polyomavirus (APV) are the most common viral diseases in psittacine birds, both affecting feathers and physical appearance of birds. Between 2005 and 2009, a total of 269 samples were collected from birds presented at veterinary clinics, shelters and rescue centers of wildlife in Costa Rica. They belonged to 19 species of psittacine birds. The most representative species in the sample were Ara macao (157), Ara ambigua (37), Amazona autumnalis (24), Amazon ochrocephala (21) and Ara ararauna (8). A prevalence of 19.7% (53/269) for PBFDV and 4.8% (13/269) for APV was determined using Polymerase Chain Reaction (PCR). In 3.3% (9/269) of the birds mixed infections were detected. Statistical analysis determined that psittacines living in shelters and rescue centers had a greater risk to be positive to PBFDV and APV than birds that were presented at veterinary clinics, while only for PBFDV it was determined, that it is more likely to detect it in feathers than in blood. Finally, birds infected with PBFDV had 6.24 times more probability to become infected with APV, than non-infected birds. This is the first report of prevalence of PBFDV and APV in captive psittacines from Costa Rica.
基金supported by the National Key Research and Development Program of China(Grant number 2016YFC0300803)the National Natural Science Foundation of China(41406182,41306169 and 41422604)+1 种基金the biodiversity investigation,observation and assessment program(2019-2023)of Ministry of Ecology and Environment of ChinaOcean Park Conservation Foundation Hong Kong(MM02-1516,AW02-1920).
文摘Beaked whales represent around 25%of known extant cetacean species,yet they are the least known of all marine mammals.Identification of many Mesoplodon species has relied on examination of a few stranded individuals.Particularly,the ginkgo-toothed beaked whale(Mesoplodon ginkgodens)and Deraniyagala’s beaked whale(Mesoplodon hotaula)are among the least-known of beaked whale species,without confirmed sightings of living individuals to date.We present a sighting of 3 free-ranging individuals of M.ginkgodens/hotaula whale from a dedicated marine mammal vessel survey carried out in the South China Sea in April and May 2019.Photographic data(301 photographs)from the sighting were compared to photos of fresh stranded ginkgo-toothed beaked whale and Deraniyagala’s beaked whale from both historical and unpublished records.We found that free-ranging M.ginkgodens and M.hotaula individuals can be easily distinguished from other Mesoplodon species due to differences in melon and gape shapes and coloration patterns.However,accurate at-sea differentiation of M.ginkgodens and M.hotaula may not be possible due to high similarity in both coloration and scarring patterns.In addition to our photo-identification data,we collected what we believe to be the first preliminary descriptions of surfacing behavior and diving patterns of one of these species.Finally,the presence of scars possibly caused by fishing gear or marine litter raises concerns about anthropogenic impacts and conservation of these poorly known species.
