Multiplex ultrasensitive detection of low abundance proteins remains a significant challenge in clinical applications,necessitating the development of innovative solutions.The integration of bead-based microfluidic ch...Multiplex ultrasensitive detection of low abundance proteins remains a significant challenge in clinical applications,necessitating the development of innovative solutions.The integration of bead-based microfluidic chip platforms with their efficient target capture and separation capabilities,along with the advantages of miniaturization and low reagent consumption,holds great promise for building an integrated point-of-care testing(POCT)system that enables seamless sample input-result output.This review presents a comprehensive overview of recent advancements in bead-based microfluidic platforms for multiplex and ultrasensitive immunoassays,along with their potential applications in clinical diagnosis and treatment,which is organized into four sections:encoding techniques,the role of microfluidic platforms,applications,and future prospects.展开更多
Electrochemiluminescence(ECL)is nowadays a powerful technique widely used in biosensing and imaging,offering high sensitivity and specificity for detecting and mapping biomolecules.Screen-printed electrodes(SPEs)offer...Electrochemiluminescence(ECL)is nowadays a powerful technique widely used in biosensing and imaging,offering high sensitivity and specificity for detecting and mapping biomolecules.Screen-printed electrodes(SPEs)offer a versatile and cost-effective platform for ECL applications due to their ease of fabrication,disposability,and suitability for large-scale production.This research introduces a novel method for improving the ECL characteristics of screen-printed carbon electrodes(SPCEs)through the application of CO_(2)laser treatment following fabrication.Using advanced ECL microscopy,we analyze three distinct carbon paste-based electrodes and show that low-energy laser exposure(ranging from 7 to 12 mJ·cm^(−2))enhances the electrochemical performance of the electrodes.This enhancement results from the selective removal of surface binders and contaminants achieved by the laser treatment.We employed ECL microscopy to characterize the ECL emission using a bead-based system incorporating magnetic microbeads,like those used in commercial platforms.This approach enabled highresolution spatial mapping of the electrode surface,offering valuable insights into its electrochemical performance.Through quantitative assessment using a photomultiplier tube(PMT),it was observed that GST electrodes could detect biomarkers with high sensitivity,achieving an approximate detection limit(LOD)of 11 antibodies perμm^(2).These findings emphasize the potential of laser-modified GST electrodes in enabling highly sensitive electrochemiluminescent immunoassays and various biosensing applications.展开更多
基金supported by the National Natural Science Foundation of China[grant numbers 32001067,82272122,and 31927803]Inter-disciplinary Program of Shanghai Jiao Tong University[grant number YG2022ZD028]Major Projects of Special Development Funds for Shanghai Zhangjiang National Innovation Demonstration Zone[grant number ZJ2021-ZD-007].
文摘Multiplex ultrasensitive detection of low abundance proteins remains a significant challenge in clinical applications,necessitating the development of innovative solutions.The integration of bead-based microfluidic chip platforms with their efficient target capture and separation capabilities,along with the advantages of miniaturization and low reagent consumption,holds great promise for building an integrated point-of-care testing(POCT)system that enables seamless sample input-result output.This review presents a comprehensive overview of recent advancements in bead-based microfluidic platforms for multiplex and ultrasensitive immunoassays,along with their potential applications in clinical diagnosis and treatment,which is organized into four sections:encoding techniques,the role of microfluidic platforms,applications,and future prospects.
基金the European Union−Next Generation EU,project MEET codice grant number 20225P4EJC,CUP J53D23014570001support from the Spanish Ministry of Science and Innovation through programs RED2022-134120-T and PID2020-113154RB-C22supported by the Nano-ImmunoEra project that has received funding from the European Union’s MSCA Staff exchange Horizon Europe programme Grant Agreement Number 101086341.
文摘Electrochemiluminescence(ECL)is nowadays a powerful technique widely used in biosensing and imaging,offering high sensitivity and specificity for detecting and mapping biomolecules.Screen-printed electrodes(SPEs)offer a versatile and cost-effective platform for ECL applications due to their ease of fabrication,disposability,and suitability for large-scale production.This research introduces a novel method for improving the ECL characteristics of screen-printed carbon electrodes(SPCEs)through the application of CO_(2)laser treatment following fabrication.Using advanced ECL microscopy,we analyze three distinct carbon paste-based electrodes and show that low-energy laser exposure(ranging from 7 to 12 mJ·cm^(−2))enhances the electrochemical performance of the electrodes.This enhancement results from the selective removal of surface binders and contaminants achieved by the laser treatment.We employed ECL microscopy to characterize the ECL emission using a bead-based system incorporating magnetic microbeads,like those used in commercial platforms.This approach enabled highresolution spatial mapping of the electrode surface,offering valuable insights into its electrochemical performance.Through quantitative assessment using a photomultiplier tube(PMT),it was observed that GST electrodes could detect biomarkers with high sensitivity,achieving an approximate detection limit(LOD)of 11 antibodies perμm^(2).These findings emphasize the potential of laser-modified GST electrodes in enabling highly sensitive electrochemiluminescent immunoassays and various biosensing applications.
