The tafoni that develop in sandstone cliffs have attracted the interest of both scientists and the general public. A necklace-like tafone system, referred to here as beaded tafoni, has developed in the prominent cliff...The tafoni that develop in sandstone cliffs have attracted the interest of both scientists and the general public. A necklace-like tafone system, referred to here as beaded tafoni, has developed in the prominent cliffs of the Danxia landscapes within the Longhushan Global Geopark in the subtropical zone of South China. This paper presents a new model of the formation of this system of extraordinary beaded tafoni. The cliffs of the Danxia landscapes of the study area are composed of an alluvial conglomerate(i.e.,red beds). These Danxia landscapes have subrounded summits that are covered by vegetation and experience a nearly vertical water flow induced by gravity. Erosion and collapse of the outsized gravels and concentrated pebbles in the red beds give rise to the initial development of the beaded tafoni. The tafoni then become rounded and beaded as a result of reworking and decay by fluvial outwash. During storms, intense water flows run vertically down the cliffs and generate a whirling motion in the tafoni.Consequently, the inside walls of the tafoni gradually become wider and smoother. During the late development stage, the beaded tafoni tend to become indistinct or disappear because of the interconnection of the tafoni and subsequent merging with the bedding-controlled cavities.展开更多
Embedding particle drugs in beaded nanofibers by electrospinning has been shown a potential approach to control drug release in tissue engineering. The bead size is one of the critical parameters in controlling the dr...Embedding particle drugs in beaded nanofibers by electrospinning has been shown a potential approach to control drug release in tissue engineering. The bead size is one of the critical parameters in controlling the drug release rate. In this study,the relationship between polymer concentration and beads size was investigated. Aqueous polyethylene oxide( PEO) solutions with different concentrations were prepared to obtain various beaded nanofibers by electrospnning. Optical microscope and scanning electron microscope( SEM) were used to observe the variation tendency of bead size. With an increase in the polymer concentration,the diameter of fibers between beads became bigger,while the fiber uniformity improved. In addition, the polymer concentration influenced the distribution of bead diameter. Higher polymer concentration would reduce the possibility of small-sized beads formation and improve the uniformity of bead diameter. The study provides a possible way to control the size of beads,which is helpful for further research on the control of particle drug release.展开更多
Poly(vinyl alcohol) (PVA) fiber mats containing unsaturated heteropoly salt was prepared for the first time. IR, X-ray diffraction and SEM photographs characterized the beaded fiber mats. The viscoelasticity and the c...Poly(vinyl alcohol) (PVA) fiber mats containing unsaturated heteropoly salt was prepared for the first time. IR, X-ray diffraction and SEM photographs characterized the beaded fiber mats. The viscoelasticity and the conductivity of the solution were the key factors that influence the formation of the beaded fiber mats.展开更多
The extracellular vesicles show great potential as a noninvasive biomarker for the early detection of cancer.Hence,there is an urgent requirement to create biosensors that are time-saving,simple,and easily scalable in...The extracellular vesicles show great potential as a noninvasive biomarker for the early detection of cancer.Hence,there is an urgent requirement to create biosensors that are time-saving,simple,and easily scalable in order to accomplish rapid,sensitive,and quantitative detection of extracellular vesicles.In this study,we present a self-propelled DNA walker powered by endonuclease Nt.Bbv CI,which enables the development of a“signal on”sensing platform for the rapid and highly sensitive detection of extracellular vesicles.The DNA motor employed tracks made of streptavidin magnetic beads,which consisted of substrate strands labeled with fluorescein and motor strands locked by aptamers.The aptamer recognition of the target protein on extracellular vesicles unlocked the motor strand,initiating the DNA motor process.After replacing the optimal buffer solution containing the endonuclease Nt.BbvC I,the motor strands autonomously moved along the streptavidin magnetic beads track,continuously releasing fluorescent molecules and producing detectable fluorescence signals.Under optimal conditions,the detection range was from 2×10~4particles/mL to 2×10~9particles/mL,with a detection limit of 2.9×10~3particles/mL,demonstrating excellent selectivity.This method has demonstrated good selectivity in different tumorderived extracellular vesicles and performs well in complex biological samples.The ability to effectively analyze surface proteins of extracellular vesicles in a short period of time gives our DNA walker a tremendous potential for developing simple and cost-effective clinical diagnostic devices.展开更多
BACKGROUND Transarterial chemoembolization(TACE)is a widely accepted palliative therapy modality for unresectable hepatocellular carcinoma(HCC).Although it is rarely curative,complete radiological response can be achi...BACKGROUND Transarterial chemoembolization(TACE)is a widely accepted palliative therapy modality for unresectable hepatocellular carcinoma(HCC).Although it is rarely curative,complete radiological response can be achieved in selected patients,leading to prolonged survival.Post-treatment tumoral calcification is an uncom-mon imaging finding in HCC and is rarely reported after drug-eluting beads TACE(DEB-TACE).CASE SUMMARY Two patients with large,solitary HCCs(>5 cm)were treated with DEB-TACE,and both achieved complete radiological response after two treatment sessions.Approximately 1 year after DEB-TACE,imaging demonstrated progressive peripheral tumoral calcification.Over 6 years of follow-up,both patients remained in remission with preserved liver function.CONCLUSION These two cases highlight the potential for complete remission and long-term survival in selected patients with large HCC following DEB-TACE.The appearance of peripheral calcification may represent a late imaging marker of effective tumor necrosis and durable treatment response although prospective studies are warranted to clarify its prognostic value.展开更多
In modern engineering,enhancing boiling heat transfer efficiency is crucial for optimizing energy use and several industrial processes involving different types of materials.This study explores the enhancement of pool...In modern engineering,enhancing boiling heat transfer efficiency is crucial for optimizing energy use and several industrial processes involving different types of materials.This study explores the enhancement of pool boiling heat transfer potentially induced by combining perforated copper particles on a heated surface with a sodium dodecyl sulfate(SDS)surfactant in saturated deionized water.Experiments were conducted at standard atmospheric pressure,with heat flux ranging from 20 to 100 kW/m2.The heating surface,positioned below the layer of freely moving copper beads,allowed the particle layer to shift due to liquid convection and steam nucleation.The study reports on the influence of copper bead diameter(2,3,4,and 5 mm),particle quantity,arrangement,and SDS concentration(20,200,and 500 ppm).It is shown that the combination of 5 mm particles and a 500 ppm SDS concentration can yield a remarkable 139%improvement in heat transfer efficiency.As demonstrated by direct flow visualization,bubble formation occurs primarily in the gaps between the particles and the heated surface,with the presence of SDS reducing bubble size and accelerating bubble detachment.展开更多
Ultrasensitive detection of multiple diseases markers is of great importance in improving diagnostic accuracy,precision,and efficiency.A versatile Au nanozyme Raman probe strategy was employed to develop an ultrasensi...Ultrasensitive detection of multiple diseases markers is of great importance in improving diagnostic accuracy,precision,and efficiency.A versatile Au nanozyme Raman probe strategy was employed to develop an ultrasensitive multiplex surface-enhanced Raman scattering(SERS)immunosensor using encoded silica photonic crystal beads(SPCBs).The efficient Au nanozyme Raman probe strategy was constructed using a robust Au nanozyme with high dual enzyme-like activity and SERS activity.On the one hand,Au nanozyme tags with oxidase-like activity can catalyze the oxidation of Raman-inactive 3,3,5,5-tetramethylbenzidine(TMB)to Raman-active oxidized TMB(ox-TMB)in the presence of O_(2).On the other hand,Au nanozyme tags with peroxidase-like activity can catalyze Raman-inactive TMB to Ramanactive ox-TMB in the presence of H_(2)O_(2).This dual catalysis action results in many Raman-active reporter molecules(ox-TMB)enabling highly sensitive detection.Meanwhile,the Au nanozyme as an extraordinary SERS substrate further enhances the detection signals of these Raman reporter molecules.Using reflection peaks of different SPCBs to encode tumor markers,an ultrasensitive multiplex SERS immunosensor was developed for detection of carcinoembryonic antigen(CEA)and alpha-fetoprotein(AFP),which exhibited wide linear ranges of 0.001-100 ng/m L for CEA and 0.01-1000 ng/m L for AFP,accompanied by low detection limits of 0.66 pg/m L for CEA and 9.5 pg/m L for AFP,respectively.This work demonstrates a universal and promising nanozyme Raman probe strategy to develop ultrasensitive multiplex SERS immunosensors for precise clinical diagnosis of disease.展开更多
[Objective]The aim of this work was to establish an analytical method for the determination of deoxynivalenol(DON)in feeds using automatic immunomagnetic beads(IMBs)clean-up coupled with high-performance liquid chroma...[Objective]The aim of this work was to establish an analytical method for the determination of deoxynivalenol(DON)in feeds using automatic immunomagnetic beads(IMBs)clean-up coupled with high-performance liquid chromatography.[Method]Feed samples were extracted using ultra-pure water,purified by automatic IMBs,and subsequently analyzed via high-performance liquid chromatography,employing an external standard method for quantification.[Result]A satisfactory linearity was achieved for DON within the concentration range of 0.05 to 2.0μg/mL,with the corresponding correlation coefficients(R^(2))exceeding 0.9999.The limit of detection(LOD)and limit of quantification(LOQ)for the proposed method were determined to be 0.03 and 0.1 mg/kg,respectively.The average recoveries of the fortified samples(0.1,0.2 and 1.0 mg/kg)were 88.5%−100.6%,with the relative standard deviations(RSD)ranging from 2.1%to 9.7%.[Conclusion]In comparison with the traditional solidphase extraction and immunoaffinity column purification methods,the IMBs technique consolidates the extraction,separation,and purification into a single process.This approach enables fully automated processing,which significantly enhances work efficiency and mitigates result deviations that may arise from manual operations.Consequently,this technique is particularly well-suited for the determination of DON in a large number of feed samples.展开更多
Common bunt is a major disease of wheat worldwide that reduces crop yields and grain quality.Rapid and sensitive quantitative detection methods are required to diagnose and monitor this disease in wheat management pro...Common bunt is a major disease of wheat worldwide that reduces crop yields and grain quality.Rapid and sensitive quantitative detection methods are required to diagnose and monitor this disease in wheat management programs and to ensure seed quality.In this study,an immunomagnetic beads-based enzyme-linked immunosorbent assay(IMBs-ELISA)was developed for the detection of Tilletia foetida teliospores in wheat and flour.An anti-T.foetida teliospores polyclonal antibody bound to immunomagnetic beads was used as the capture probe,and a polyclonal antibody labeled with horseradish peroxidase was used as the detector probe.The capture and detection conditions for the target spores were optimized to achieve the best determination results.Under optimal conditions,the proposed method took less than 2 h to complete.Its limit of detection was 300 teliospores per gram.The critical reaction in this IMBs-ELISA occurred on magnetic beads.This not only simplified the traditional ELISA process,but also shortened the detection time.This study has expanded the application of the IMBs-ELISA method to fungal spore detection.This method has potential applications in agriculture and seed management.展开更多
Esox reieherti Dybowsk genomic microsateUites were developed by using enrichment protocols combined with radioactive hybridization protocol. Four hundred to nine hundred base pair fragments were selected for the whole...Esox reieherti Dybowsk genomic microsateUites were developed by using enrichment protocols combined with radioactive hybridization protocol. Four hundred to nine hundred base pair fragments were selected for the whole genome. DNA PCR amplification after digestion with restriction endonuclease Sau 3A Ⅰ, and （CA）12, （GA）12 probes marked with biotin were used for microsateUite DNA enrichment. The product fragments were connected with carder pGEM-T and transferred into DH5α Escherichia coli competent cells, and radioactive isotope probes marked with γ^-32 p were used for the second hybridization. As a result, a total of 1600 bacteria were obtained in the microsatellite genomic libraries, positive clones accounted for 90.91% before hybridization and 81.25% after hybridization, amounting to 1300. One hundred and ninety-six positive clones were selected for sequencing, and 192 clones included microsateUite sequences. The microsateUite sequences obtained, mono-nucleotide, quad-nucleotide and quint-nucleotide repeat motifs were observed beside double-base-pairs CA/GT, GA/CT. Seventy primers were designed according to the flanking sequences by using software Primer Premier 5.0, and 32 primers were selected to be synthesized. After optimizing PCR reaction conditions, 28 primers were amplified and produced clear purpose bands. The aim of our research was to promote the development and utilization of E. reieherti genomic resource, and lay the foundation for optimizing E. reieherti breeding strain in order to detect the genetic diversity and construct a genetic map.展开更多
A TiO2 photocatalyst is coated on the surface of a zeolite fly ash bead（ZFAB） to improve its dispersability and exposure degree in a cement system.The application of Ag particles in TiO2/ZFAB modified cementitious m...A TiO2 photocatalyst is coated on the surface of a zeolite fly ash bead（ZFAB） to improve its dispersability and exposure degree in a cement system.The application of Ag particles in TiO2/ZFAB modified cementitious materials is to further enhance the photocatalytic performance.Various Ag@TiO2/ZFAB modified cementitious specimens with different Ag dosages are prepared and the characteristics and photocatalytic performance of the prepared samples are investigated.It is observed that the multi-level pore structure of ZFAB can improve the exposure degree of TiO2 in a cement system and is also useful to enhance the photocatalytic efficiency.With an increment of the amounts of Ag particles in the TiO2/ZFAB modified cementitious samples,the photocatalytic activities increased first and then decreased.The optimal Ag@TiO2/ZFAB modified cementitious sample reveals the maximum reaction rate constant for degrading benzene（9.91×10^-3 min^-1）,which is approximately 3 and 10 times higher than those of TiO2/ZFAB and TiO2 modified samples,respectively.This suggests that suitable Ag particles coupled with a ZFAB carrier could effectively enhance the photocatalytic effects and use of TiO2 in a cement system.Thus,ZFAB as a carrier could provide a potential method for a high efficiency engineering application of TiO2 in the construction field.展开更多
To investigate influence of welding parameters on weld bead geometry in underwater wet flux cored arc welding （FCAW）, orthogonal experiments of underwater wet FCAW were conducted in the hyperbaric chamber at water d...To investigate influence of welding parameters on weld bead geometry in underwater wet flux cored arc welding （FCAW）, orthogonal experiments of underwater wet FCAW were conducted in the hyperbaric chamber at water depth from 0.2 m to 60 m and mathematical models were developed by multiple curvilinear regression method from the experimental data. Sensitivity analysis was then performed to predict the bead geometry and evaluate the influence of welding parameters. The results reveal that water depth has a greater influence on bead geometry than other welding parameters when welding at a water depth less than 10 m. At a water depth deeper than 10 m, a change in travel speed affects the bead geometry more strongly than other welding parameters.展开更多
Chest CT frequently reveals small pulmonary lesions. If there is no associated hilar or mediastinal lymphadenopathy, small-cell lung cancer (SCLC) is considered to be very early-stage. Few studies, however, have descr...Chest CT frequently reveals small pulmonary lesions. If there is no associated hilar or mediastinal lymphadenopathy, small-cell lung cancer (SCLC) is considered to be very early-stage. Few studies, however, have described the characteristic CT findings of very early-stage SCLC. Clarifying these findings would reduce diagnostic delay. The purpose of this study was to determine the characteristic CT findings of very earlystage SCLC. Computed tomography images obtained from 547 consecutive patients with lung cancer between 2003 and 2012 at Tokyo Dental College Ichikawa General Hospital were reviewed retrospectively. One hundred of these patients had SCLC and 447 had non-SCLC (NSCLC). These CT images, along with any that had also been obtained prior to the one on which the final diagnosis was based, were reviewed. In 5 of the cases reviewed, specific findings and a characteristic spreading pattern were identified on CT images that might have allowed a diagnosis of SCLC to have been made sooner. The findings included a sub-pleural, small nodule accompanied by daughter nodules spreading longitudinally along the bronchovascular bundle, no air bronchogram or cavitation, and background emphysematous change. These were not identified on CT images of very earlystage NCSLC, however (n = 22). Awareness of the relevance of these findings would help physicians and radiologists arrive at a differential diagnosis of small pulmonary nodules.展开更多
基金financially supported by National Natural Science Foundation of China (Grant Nos. 41772197, 41602113)Open Research Fund from the Shandong Provincial Key Laboratory of Depositional Mineralization & Sedimentary Minerals (Shandong University of Science and Technology+1 种基金 Grant No. DMSM2017011)Jiangxi Provincial Graduate Innovation Fund Project (YC2018-S336)
文摘The tafoni that develop in sandstone cliffs have attracted the interest of both scientists and the general public. A necklace-like tafone system, referred to here as beaded tafoni, has developed in the prominent cliffs of the Danxia landscapes within the Longhushan Global Geopark in the subtropical zone of South China. This paper presents a new model of the formation of this system of extraordinary beaded tafoni. The cliffs of the Danxia landscapes of the study area are composed of an alluvial conglomerate(i.e.,red beds). These Danxia landscapes have subrounded summits that are covered by vegetation and experience a nearly vertical water flow induced by gravity. Erosion and collapse of the outsized gravels and concentrated pebbles in the red beds give rise to the initial development of the beaded tafoni. The tafoni then become rounded and beaded as a result of reworking and decay by fluvial outwash. During storms, intense water flows run vertically down the cliffs and generate a whirling motion in the tafoni.Consequently, the inside walls of the tafoni gradually become wider and smoother. During the late development stage, the beaded tafoni tend to become indistinct or disappear because of the interconnection of the tafoni and subsequent merging with the bedding-controlled cavities.
基金Textile Biomaterials Science and Technology Innovation Intelligence(111 Project),China(No.B07024)
文摘Embedding particle drugs in beaded nanofibers by electrospinning has been shown a potential approach to control drug release in tissue engineering. The bead size is one of the critical parameters in controlling the drug release rate. In this study,the relationship between polymer concentration and beads size was investigated. Aqueous polyethylene oxide( PEO) solutions with different concentrations were prepared to obtain various beaded nanofibers by electrospnning. Optical microscope and scanning electron microscope( SEM) were used to observe the variation tendency of bead size. With an increase in the polymer concentration,the diameter of fibers between beads became bigger,while the fiber uniformity improved. In addition, the polymer concentration influenced the distribution of bead diameter. Higher polymer concentration would reduce the possibility of small-sized beads formation and improve the uniformity of bead diameter. The study provides a possible way to control the size of beads,which is helpful for further research on the control of particle drug release.
文摘Poly(vinyl alcohol) (PVA) fiber mats containing unsaturated heteropoly salt was prepared for the first time. IR, X-ray diffraction and SEM photographs characterized the beaded fiber mats. The viscoelasticity and the conductivity of the solution were the key factors that influence the formation of the beaded fiber mats.
基金supported financially by the National Natural Science Foundation of China(NSFC,Nos.62071119 and 62075098)the National Key Research and Development Program of China(Nos.2017YFA0205301 and 2018YFC1602905)。
文摘The extracellular vesicles show great potential as a noninvasive biomarker for the early detection of cancer.Hence,there is an urgent requirement to create biosensors that are time-saving,simple,and easily scalable in order to accomplish rapid,sensitive,and quantitative detection of extracellular vesicles.In this study,we present a self-propelled DNA walker powered by endonuclease Nt.Bbv CI,which enables the development of a“signal on”sensing platform for the rapid and highly sensitive detection of extracellular vesicles.The DNA motor employed tracks made of streptavidin magnetic beads,which consisted of substrate strands labeled with fluorescein and motor strands locked by aptamers.The aptamer recognition of the target protein on extracellular vesicles unlocked the motor strand,initiating the DNA motor process.After replacing the optimal buffer solution containing the endonuclease Nt.BbvC I,the motor strands autonomously moved along the streptavidin magnetic beads track,continuously releasing fluorescent molecules and producing detectable fluorescence signals.Under optimal conditions,the detection range was from 2×10~4particles/mL to 2×10~9particles/mL,with a detection limit of 2.9×10~3particles/mL,demonstrating excellent selectivity.This method has demonstrated good selectivity in different tumorderived extracellular vesicles and performs well in complex biological samples.The ability to effectively analyze surface proteins of extracellular vesicles in a short period of time gives our DNA walker a tremendous potential for developing simple and cost-effective clinical diagnostic devices.
文摘BACKGROUND Transarterial chemoembolization(TACE)is a widely accepted palliative therapy modality for unresectable hepatocellular carcinoma(HCC).Although it is rarely curative,complete radiological response can be achieved in selected patients,leading to prolonged survival.Post-treatment tumoral calcification is an uncom-mon imaging finding in HCC and is rarely reported after drug-eluting beads TACE(DEB-TACE).CASE SUMMARY Two patients with large,solitary HCCs(>5 cm)were treated with DEB-TACE,and both achieved complete radiological response after two treatment sessions.Approximately 1 year after DEB-TACE,imaging demonstrated progressive peripheral tumoral calcification.Over 6 years of follow-up,both patients remained in remission with preserved liver function.CONCLUSION These two cases highlight the potential for complete remission and long-term survival in selected patients with large HCC following DEB-TACE.The appearance of peripheral calcification may represent a late imaging marker of effective tumor necrosis and durable treatment response although prospective studies are warranted to clarify its prognostic value.
基金supported by the National Natural Science Foundation of China(Project No.52166004)the National Key Research and Development Program of China(Project No.2022YFC3902000)+2 种基金the Major Science and Technology Special Project of Yunnan Province(Project Nos.202202AG050007202202AG050002)the Research on the Development of Complete Sets of Technology for Extraction of Aromatic Substances from Tobacco Waste and Its Application,Applied Research-Pyrolysis Process Technology Research(2023QT01).
文摘In modern engineering,enhancing boiling heat transfer efficiency is crucial for optimizing energy use and several industrial processes involving different types of materials.This study explores the enhancement of pool boiling heat transfer potentially induced by combining perforated copper particles on a heated surface with a sodium dodecyl sulfate(SDS)surfactant in saturated deionized water.Experiments were conducted at standard atmospheric pressure,with heat flux ranging from 20 to 100 kW/m2.The heating surface,positioned below the layer of freely moving copper beads,allowed the particle layer to shift due to liquid convection and steam nucleation.The study reports on the influence of copper bead diameter(2,3,4,and 5 mm),particle quantity,arrangement,and SDS concentration(20,200,and 500 ppm).It is shown that the combination of 5 mm particles and a 500 ppm SDS concentration can yield a remarkable 139%improvement in heat transfer efficiency.As demonstrated by direct flow visualization,bubble formation occurs primarily in the gaps between the particles and the heated surface,with the presence of SDS reducing bubble size and accelerating bubble detachment.
基金financially supported by National Natural Science Foundation of China(Nos.21475116,21575125 and 22474124)the National Natural Science Foundation of Jiangsu Province(Nos.BK20221370,BK20211362)+5 种基金Key University Natural Science Foundation of Jiangsu-Province(No.20KJA150004)the Project for Science and Technology of Yangzhou(No.YZ2022074)the Project for Yangzhou City and Yangzhou University corporation(No.YZ2023204)Cross cooperation project of Subei Peoples’Hospital of Jiangsu Province(No.SBJC220009)the Open Research Fund of State Key Laboratory of Analytical Chemistry for Life Science(No.SKLACLS2405)Postgraduate Research&Practice Innovation Program of Jiangsu Province(No.KYCX24_3728)。
文摘Ultrasensitive detection of multiple diseases markers is of great importance in improving diagnostic accuracy,precision,and efficiency.A versatile Au nanozyme Raman probe strategy was employed to develop an ultrasensitive multiplex surface-enhanced Raman scattering(SERS)immunosensor using encoded silica photonic crystal beads(SPCBs).The efficient Au nanozyme Raman probe strategy was constructed using a robust Au nanozyme with high dual enzyme-like activity and SERS activity.On the one hand,Au nanozyme tags with oxidase-like activity can catalyze the oxidation of Raman-inactive 3,3,5,5-tetramethylbenzidine(TMB)to Raman-active oxidized TMB(ox-TMB)in the presence of O_(2).On the other hand,Au nanozyme tags with peroxidase-like activity can catalyze Raman-inactive TMB to Ramanactive ox-TMB in the presence of H_(2)O_(2).This dual catalysis action results in many Raman-active reporter molecules(ox-TMB)enabling highly sensitive detection.Meanwhile,the Au nanozyme as an extraordinary SERS substrate further enhances the detection signals of these Raman reporter molecules.Using reflection peaks of different SPCBs to encode tumor markers,an ultrasensitive multiplex SERS immunosensor was developed for detection of carcinoembryonic antigen(CEA)and alpha-fetoprotein(AFP),which exhibited wide linear ranges of 0.001-100 ng/m L for CEA and 0.01-1000 ng/m L for AFP,accompanied by low detection limits of 0.66 pg/m L for CEA and 9.5 pg/m L for AFP,respectively.This work demonstrates a universal and promising nanozyme Raman probe strategy to develop ultrasensitive multiplex SERS immunosensors for precise clinical diagnosis of disease.
基金Supported by National Key Research and Development Program of China(2023YFD1301001)Central Public-interest Scientific Institution Basal Research Fund(1610072023005)Agricultural Science and Technology Innovation Program of CAAS(CAAS-ASTIP-IQSTAP-04).
文摘[Objective]The aim of this work was to establish an analytical method for the determination of deoxynivalenol(DON)in feeds using automatic immunomagnetic beads(IMBs)clean-up coupled with high-performance liquid chromatography.[Method]Feed samples were extracted using ultra-pure water,purified by automatic IMBs,and subsequently analyzed via high-performance liquid chromatography,employing an external standard method for quantification.[Result]A satisfactory linearity was achieved for DON within the concentration range of 0.05 to 2.0μg/mL,with the corresponding correlation coefficients(R^(2))exceeding 0.9999.The limit of detection(LOD)and limit of quantification(LOQ)for the proposed method were determined to be 0.03 and 0.1 mg/kg,respectively.The average recoveries of the fortified samples(0.1,0.2 and 1.0 mg/kg)were 88.5%−100.6%,with the relative standard deviations(RSD)ranging from 2.1%to 9.7%.[Conclusion]In comparison with the traditional solidphase extraction and immunoaffinity column purification methods,the IMBs technique consolidates the extraction,separation,and purification into a single process.This approach enables fully automated processing,which significantly enhances work efficiency and mitigates result deviations that may arise from manual operations.Consequently,this technique is particularly well-suited for the determination of DON in a large number of feed samples.
基金supported by the National Key Research&Development Program(2024YFF0618103,2023YFF0611503,2023YFF1105102)the Science and Technology Program of State Administration for Market Regulation(2024MK170)+1 种基金the Science and Technology Project of Jiangsu Market Supervision and Administration(KJ2024014,KJ2024059)the Science and Technology Program of Nanjing Market Supervision and Administration(Kj2023006).
文摘Common bunt is a major disease of wheat worldwide that reduces crop yields and grain quality.Rapid and sensitive quantitative detection methods are required to diagnose and monitor this disease in wheat management programs and to ensure seed quality.In this study,an immunomagnetic beads-based enzyme-linked immunosorbent assay(IMBs-ELISA)was developed for the detection of Tilletia foetida teliospores in wheat and flour.An anti-T.foetida teliospores polyclonal antibody bound to immunomagnetic beads was used as the capture probe,and a polyclonal antibody labeled with horseradish peroxidase was used as the detector probe.The capture and detection conditions for the target spores were optimized to achieve the best determination results.Under optimal conditions,the proposed method took less than 2 h to complete.Its limit of detection was 300 teliospores per gram.The critical reaction in this IMBs-ELISA occurred on magnetic beads.This not only simplified the traditional ELISA process,but also shortened the detection time.This study has expanded the application of the IMBs-ELISA method to fungal spore detection.This method has potential applications in agriculture and seed management.
文摘Esox reieherti Dybowsk genomic microsateUites were developed by using enrichment protocols combined with radioactive hybridization protocol. Four hundred to nine hundred base pair fragments were selected for the whole genome. DNA PCR amplification after digestion with restriction endonuclease Sau 3A Ⅰ, and （CA）12, （GA）12 probes marked with biotin were used for microsateUite DNA enrichment. The product fragments were connected with carder pGEM-T and transferred into DH5α Escherichia coli competent cells, and radioactive isotope probes marked with γ^-32 p were used for the second hybridization. As a result, a total of 1600 bacteria were obtained in the microsatellite genomic libraries, positive clones accounted for 90.91% before hybridization and 81.25% after hybridization, amounting to 1300. One hundred and ninety-six positive clones were selected for sequencing, and 192 clones included microsateUite sequences. The microsateUite sequences obtained, mono-nucleotide, quad-nucleotide and quint-nucleotide repeat motifs were observed beside double-base-pairs CA/GT, GA/CT. Seventy primers were designed according to the flanking sequences by using software Primer Premier 5.0, and 32 primers were selected to be synthesized. After optimizing PCR reaction conditions, 28 primers were amplified and produced clear purpose bands. The aim of our research was to promote the development and utilization of E. reieherti genomic resource, and lay the foundation for optimizing E. reieherti breeding strain in order to detect the genetic diversity and construct a genetic map.
基金supported by the National Natural Science Foundation of China (51478370)the Engineering and Physical Sciences Research Council of UK–Natural Science Foundation of China (EPSRC-NSFC) International Joint Research Project (51461135005)~~
文摘A TiO2 photocatalyst is coated on the surface of a zeolite fly ash bead（ZFAB） to improve its dispersability and exposure degree in a cement system.The application of Ag particles in TiO2/ZFAB modified cementitious materials is to further enhance the photocatalytic performance.Various Ag@TiO2/ZFAB modified cementitious specimens with different Ag dosages are prepared and the characteristics and photocatalytic performance of the prepared samples are investigated.It is observed that the multi-level pore structure of ZFAB can improve the exposure degree of TiO2 in a cement system and is also useful to enhance the photocatalytic efficiency.With an increment of the amounts of Ag particles in the TiO2/ZFAB modified cementitious samples,the photocatalytic activities increased first and then decreased.The optimal Ag@TiO2/ZFAB modified cementitious sample reveals the maximum reaction rate constant for degrading benzene（9.91×10^-3 min^-1）,which is approximately 3 and 10 times higher than those of TiO2/ZFAB and TiO2 modified samples,respectively.This suggests that suitable Ag particles coupled with a ZFAB carrier could effectively enhance the photocatalytic effects and use of TiO2 in a cement system.Thus,ZFAB as a carrier could provide a potential method for a high efficiency engineering application of TiO2 in the construction field.
基金Projects(51175185,50705030)supported by the National Natural Science Foundation of ChinaProject(2012ZZ0052)supported by the Fundamental Research Funds for the Central Universities,ChinaProject(9151064101000065)supported by the Natural Science Foundation of Guangdong Province,China
文摘To investigate influence of welding parameters on weld bead geometry in underwater wet flux cored arc welding （FCAW）, orthogonal experiments of underwater wet FCAW were conducted in the hyperbaric chamber at water depth from 0.2 m to 60 m and mathematical models were developed by multiple curvilinear regression method from the experimental data. Sensitivity analysis was then performed to predict the bead geometry and evaluate the influence of welding parameters. The results reveal that water depth has a greater influence on bead geometry than other welding parameters when welding at a water depth less than 10 m. At a water depth deeper than 10 m, a change in travel speed affects the bead geometry more strongly than other welding parameters.
文摘Chest CT frequently reveals small pulmonary lesions. If there is no associated hilar or mediastinal lymphadenopathy, small-cell lung cancer (SCLC) is considered to be very early-stage. Few studies, however, have described the characteristic CT findings of very early-stage SCLC. Clarifying these findings would reduce diagnostic delay. The purpose of this study was to determine the characteristic CT findings of very earlystage SCLC. Computed tomography images obtained from 547 consecutive patients with lung cancer between 2003 and 2012 at Tokyo Dental College Ichikawa General Hospital were reviewed retrospectively. One hundred of these patients had SCLC and 447 had non-SCLC (NSCLC). These CT images, along with any that had also been obtained prior to the one on which the final diagnosis was based, were reviewed. In 5 of the cases reviewed, specific findings and a characteristic spreading pattern were identified on CT images that might have allowed a diagnosis of SCLC to have been made sooner. The findings included a sub-pleural, small nodule accompanied by daughter nodules spreading longitudinally along the bronchovascular bundle, no air bronchogram or cavitation, and background emphysematous change. These were not identified on CT images of very earlystage NCSLC, however (n = 22). Awareness of the relevance of these findings would help physicians and radiologists arrive at a differential diagnosis of small pulmonary nodules.
