Botrytis cinerea is one of the most destructive plant pathogens,causing great economic losses in a wide variety of crops.It is difficult to control the pathogen because of its flexible mode of infection,high reproduct...Botrytis cinerea is one of the most destructive plant pathogens,causing great economic losses in a wide variety of crops.It is difficult to control the pathogen because of its flexible mode of infection,high reproduction,genetic plasticity and strong stress resistance.There are more than 400 genes encoding transcription factors in the genome of B.cinerea,among which Zn(II)2Cys6(C6)transcription factors constitute the largest family.However,the mechanisms of Zn(II)2Cys6(C6)transcription factors regulating the development and pathogenicity of B.cinerea are largely unknown.In this study,we identified an important C6 transcription factor BcDIC that regulates the asexual reproduction and pathogenicity of B.cinerea.The virulence of the knockout mutantΔBcDIC was reduced on various hosts.Especially,ΔBcDIC completely lost the ability of conidiation.By analyzing the transcriptome data,we found that deletion ofΔBcDIC enhanced the expression of pectinesterase family genes in B.cinerea.Furthermore,the results of yeastone-hybrid and dual-LUC reporter assay suggested that BcDIC could bind to the promoter of two pectinesterase genes and suppress their expression.In this work,we identified a C6 transcription factor involved in the pathogenesis and asexual reproduction of B.cinerea,and the results implied that the capacity of pectin degradation was closely related with the reproduction process.展开更多
基金supported by the Natural Science Foundation of Beijing(6222048)the National Natural Science Foundation of China(32172642,31972475)the National Key Research and Development Program of China(2021YFD2100505).
文摘Botrytis cinerea is one of the most destructive plant pathogens,causing great economic losses in a wide variety of crops.It is difficult to control the pathogen because of its flexible mode of infection,high reproduction,genetic plasticity and strong stress resistance.There are more than 400 genes encoding transcription factors in the genome of B.cinerea,among which Zn(II)2Cys6(C6)transcription factors constitute the largest family.However,the mechanisms of Zn(II)2Cys6(C6)transcription factors regulating the development and pathogenicity of B.cinerea are largely unknown.In this study,we identified an important C6 transcription factor BcDIC that regulates the asexual reproduction and pathogenicity of B.cinerea.The virulence of the knockout mutantΔBcDIC was reduced on various hosts.Especially,ΔBcDIC completely lost the ability of conidiation.By analyzing the transcriptome data,we found that deletion ofΔBcDIC enhanced the expression of pectinesterase family genes in B.cinerea.Furthermore,the results of yeastone-hybrid and dual-LUC reporter assay suggested that BcDIC could bind to the promoter of two pectinesterase genes and suppress their expression.In this work,we identified a C6 transcription factor involved in the pathogenesis and asexual reproduction of B.cinerea,and the results implied that the capacity of pectin degradation was closely related with the reproduction process.
