: The genetic diversity and genetic structure of a population of isolates of Sclerotinia sclerotiorum (Lib.) de Bary from different regions and host plants were investigated using the random amplified polymorphic DNA ...: The genetic diversity and genetic structure of a population of isolates of Sclerotinia sclerotiorum (Lib.) de Bary from different regions and host plants were investigated using the random amplified polymorphic DNA (RAPD) method with 20 random decamer primer pairs in order to provide some information on the phylogenetic taxa and breeding for resistance to sclerotinia stem rot. A minimum of three and a maximum of 15 unambiguously amplified bands were generated, furnishing a total of 170 bands ranging in size from 100 to 3 200 bp, corresponding to an average of 8.5 bands per primer pair. One hundred and four of these 170 bands (61.2%) were polymorphic, the percentage of polymorphic bands for each primer pair ranging from 0.0% to 86.7%. The genetic relationships among the isolates, based on the results of RAPD analysis, were examined. The genetic similarity of all selected isolates was quite high. At the species level, the genetic diversity estimated by Nei's gene diversity (h) was 0.197 and Shannon's index of diversity (I) was 0.300. The unweighted pair-group mean analysis (UPGMA) cluster analysis showed that most isolates from the same regions were grouped in the same cluster or a close cluster. The population of isolates from Hefei (Anhui Province, China) was more uniform and relatively distant to other populations. The Canadian population collected from carrot (Daucus carota var. sativa DC.) was relatively close to the Polish population collected from oilseed rape (Brassica napus L.) plants. There was no relationship between isolates from the same host plants. An analysis of molecular variance (AMOVA) revealed that the percentage of variance attributable to variation among and within populations was 50.62% and 49.38%, respectively. When accessions from China, Europe, and Canada were treated as three separate groups, the variance components among groups, among populations within groups, and within populations were ?0.96%, 51.48%, and 49.47%, respectively. The genetic differentiations among and within populations were highly significant (P < 0.001). Similarly, the coefficient of gene differentiation (Gst) in total populations calculated by population genetic analysis was 0.229 4, which indicated that the genetic variation among populations was 22.94%. The gene flow (Nm) was 1.68, which indicated that the gene permutation and interaction among populations was relatively high.展开更多
[ Objective] The paper was to screen lignin-degrading strains with high efiqcacy. [ Method] Through potato agar plate culture, potato liquid shake-flask culture and Bavendamm＇ s reaction, the lignin-degrading strains...[ Objective] The paper was to screen lignin-degrading strains with high efiqcacy. [ Method] Through potato agar plate culture, potato liquid shake-flask culture and Bavendamm＇ s reaction, the lignin-degrading strains collected from the dead wood in Jiwen Mountain were screened, their kappa values and cellulose for degrading papermulberry were also determined. [ Result] A total of 3 species of strains with strong degradation ability on ligin were screened out, including JWS-1, JWS-2 and Sclerotinia sclerotiorum （Lib.）de Bary. [Condusion] The study laid basis for further study on bio-pulping.展开更多
1糖尿病时心血管合并症危险明显增加
旁路血管成形术血管再通调查(BARI)比较了糖尿病与非糖尿病者血管重建术(包括经皮冠状动脉腔内成形术和冠状动脉搭桥术)后5年的病死率,结果糖尿病病人预后更差,血管重建术后5年病死率糖尿病病人是非...1糖尿病时心血管合并症危险明显增加
旁路血管成形术血管再通调查(BARI)比较了糖尿病与非糖尿病者血管重建术(包括经皮冠状动脉腔内成形术和冠状动脉搭桥术)后5年的病死率,结果糖尿病病人预后更差,血管重建术后5年病死率糖尿病病人是非糖尿病病人的2～3倍.芬兰东-西研究(East West Study)的7年队列观察结果显示,无心肌梗死(心梗)史的糖尿病病人与有心梗史的非糖尿病病人发生心梗的危险性相同,均在20%左右.制定缺血综合征治疗策略机构的研究(OASIS)发现,与无糖尿病、无心血管疾病(CVD)者的心血管事件发生危险相比,已有CVD史的糖尿病病人再发生心血管事件的危险增加3倍,而无糖尿病有CVD的病人与有糖尿病无CVD史的病人再发生心血管事件的危险相同,均增加2倍.该研究也说明,糖尿病病人与无糖尿病的CVD病人处在相同的危险水平上.展开更多
Currently the soybean crop is affected by the white mold (Sclerotinia sclerotiorum). The disease can reduce the crop yield and quality and decrease the prices of agricultural lands. The aim of the current research was...Currently the soybean crop is affected by the white mold (Sclerotinia sclerotiorum). The disease can reduce the crop yield and quality and decrease the prices of agricultural lands. The aim of the current research was to assess epidemiology of white mold on soybean crop grown at Arapoti, PR, Brazil, utilizing data related to agricultural practices and local meteorological factors. The experiment was conducted in a naturally infested area. The soybean crop was sown on October 18th, 2011. The experimental design adopted herein was a randomized block in a factorial combination with 4 row spacings (0.35, 0.45, 0.60, 0.75 m) and 4 plant populations (150, 200, 250, 300 thousand plants per hectare) and 4 replications. The temporal analysis of the epidemic was evaluated using mathematical models, such as Logistics, Monomolecular and Gompertz, in order to determine the best model that described the progress of the disease as a function of local meteorological elements. For the incidence data it has been shown that both logistic and monomolecular models were those that were best fitted to the experimental data. For severity, the best model related to the experimental data was the logistic one. Either for incidence or for severity, air temperature was considered to be the environmental factor most affecting the progress of the disease. The variability in the apparent infection rates of white mold on soybean was not affected by different row spacings and plant populations;therefore, suggesting that macroclimatic variations prevailed in such a fashion to mitigate the effect of cultural practices adopted in the field.展开更多
2型糖尿病的心血管事件发生率比非糖尿病患者要高。很少有大型的随机的试验提出这个问题——到底合并糖尿病的且经冠脉造影证实的缺血性心脏病患者的最佳治疗方案是什么？2型糖尿病中旁路血管成形术再血管化调查——一项治疗合并2型糖...2型糖尿病的心血管事件发生率比非糖尿病患者要高。很少有大型的随机的试验提出这个问题——到底合并糖尿病的且经冠脉造影证实的缺血性心脏病患者的最佳治疗方案是什么？2型糖尿病中旁路血管成形术再血管化调查——一项治疗合并2型糖尿病的冠心病的随机研究（A Randomized Trial of Therapies for Type 2 Diabetes and Coronary Artery Disease，The BARI 2D Study Group，BARI 2D）的目的就是为了评价这类患者的治疗效果。展开更多
V Loizzi等对1998～2002年在BARI大学确诊的所有因卵巢癌发生肝转移的29例患者进行了研究，其中ⅡC期1例、ⅢA期l例、ⅢB期2例，ⅢC期17例、ⅣB期8例。8例(第一组)在被确诊患卵巢癌的同时有肝转移的临床特征，10例(第二组)在第1次复发...V Loizzi等对1998～2002年在BARI大学确诊的所有因卵巢癌发生肝转移的29例患者进行了研究，其中ⅡC期1例、ⅢA期l例、ⅢB期2例，ⅢC期17例、ⅣB期8例。8例(第一组)在被确诊患卵巢癌的同时有肝转移的临床特征，10例(第二组)在第1次复发时有肝转移特征．11例(第三组)在第2次复发时有肝转移特征。从确认肝转移之日起，展开更多
基金Basic Research and Development Plan of China，国家科技攻关项目，the Sino-Polish Scientific and Technological Cooperation Project for 2004-2006，中国科学院资助项目
文摘: The genetic diversity and genetic structure of a population of isolates of Sclerotinia sclerotiorum (Lib.) de Bary from different regions and host plants were investigated using the random amplified polymorphic DNA (RAPD) method with 20 random decamer primer pairs in order to provide some information on the phylogenetic taxa and breeding for resistance to sclerotinia stem rot. A minimum of three and a maximum of 15 unambiguously amplified bands were generated, furnishing a total of 170 bands ranging in size from 100 to 3 200 bp, corresponding to an average of 8.5 bands per primer pair. One hundred and four of these 170 bands (61.2%) were polymorphic, the percentage of polymorphic bands for each primer pair ranging from 0.0% to 86.7%. The genetic relationships among the isolates, based on the results of RAPD analysis, were examined. The genetic similarity of all selected isolates was quite high. At the species level, the genetic diversity estimated by Nei's gene diversity (h) was 0.197 and Shannon's index of diversity (I) was 0.300. The unweighted pair-group mean analysis (UPGMA) cluster analysis showed that most isolates from the same regions were grouped in the same cluster or a close cluster. The population of isolates from Hefei (Anhui Province, China) was more uniform and relatively distant to other populations. The Canadian population collected from carrot (Daucus carota var. sativa DC.) was relatively close to the Polish population collected from oilseed rape (Brassica napus L.) plants. There was no relationship between isolates from the same host plants. An analysis of molecular variance (AMOVA) revealed that the percentage of variance attributable to variation among and within populations was 50.62% and 49.38%, respectively. When accessions from China, Europe, and Canada were treated as three separate groups, the variance components among groups, among populations within groups, and within populations were ?0.96%, 51.48%, and 49.47%, respectively. The genetic differentiations among and within populations were highly significant (P < 0.001). Similarly, the coefficient of gene differentiation (Gst) in total populations calculated by population genetic analysis was 0.229 4, which indicated that the genetic variation among populations was 22.94%. The gene flow (Nm) was 1.68, which indicated that the gene permutation and interaction among populations was relatively high.
基金Supported by Building Projects of Ministry of Education ( Z200721201020)High School Science Research Project of Inner Mongolia Autonomous Region ( NJzy08233)Science and Technology Project of Inner Mongolia Baotou City ( 2008y100222)~~
文摘[ Objective] The paper was to screen lignin-degrading strains with high efiqcacy. [ Method] Through potato agar plate culture, potato liquid shake-flask culture and Bavendamm＇ s reaction, the lignin-degrading strains collected from the dead wood in Jiwen Mountain were screened, their kappa values and cellulose for degrading papermulberry were also determined. [ Result] A total of 3 species of strains with strong degradation ability on ligin were screened out, including JWS-1, JWS-2 and Sclerotinia sclerotiorum （Lib.）de Bary. [Condusion] The study laid basis for further study on bio-pulping.
文摘1糖尿病时心血管合并症危险明显增加
旁路血管成形术血管再通调查(BARI)比较了糖尿病与非糖尿病者血管重建术(包括经皮冠状动脉腔内成形术和冠状动脉搭桥术)后5年的病死率,结果糖尿病病人预后更差,血管重建术后5年病死率糖尿病病人是非糖尿病病人的2～3倍.芬兰东-西研究(East West Study)的7年队列观察结果显示,无心肌梗死(心梗)史的糖尿病病人与有心梗史的非糖尿病病人发生心梗的危险性相同,均在20%左右.制定缺血综合征治疗策略机构的研究(OASIS)发现,与无糖尿病、无心血管疾病(CVD)者的心血管事件发生危险相比,已有CVD史的糖尿病病人再发生心血管事件的危险增加3倍,而无糖尿病有CVD的病人与有糖尿病无CVD史的病人再发生心血管事件的危险相同,均增加2倍.该研究也说明,糖尿病病人与无糖尿病的CVD病人处在相同的危险水平上.
文摘Currently the soybean crop is affected by the white mold (Sclerotinia sclerotiorum). The disease can reduce the crop yield and quality and decrease the prices of agricultural lands. The aim of the current research was to assess epidemiology of white mold on soybean crop grown at Arapoti, PR, Brazil, utilizing data related to agricultural practices and local meteorological factors. The experiment was conducted in a naturally infested area. The soybean crop was sown on October 18th, 2011. The experimental design adopted herein was a randomized block in a factorial combination with 4 row spacings (0.35, 0.45, 0.60, 0.75 m) and 4 plant populations (150, 200, 250, 300 thousand plants per hectare) and 4 replications. The temporal analysis of the epidemic was evaluated using mathematical models, such as Logistics, Monomolecular and Gompertz, in order to determine the best model that described the progress of the disease as a function of local meteorological elements. For the incidence data it has been shown that both logistic and monomolecular models were those that were best fitted to the experimental data. For severity, the best model related to the experimental data was the logistic one. Either for incidence or for severity, air temperature was considered to be the environmental factor most affecting the progress of the disease. The variability in the apparent infection rates of white mold on soybean was not affected by different row spacings and plant populations;therefore, suggesting that macroclimatic variations prevailed in such a fashion to mitigate the effect of cultural practices adopted in the field.
文摘2型糖尿病的心血管事件发生率比非糖尿病患者要高。很少有大型的随机的试验提出这个问题——到底合并糖尿病的且经冠脉造影证实的缺血性心脏病患者的最佳治疗方案是什么？2型糖尿病中旁路血管成形术再血管化调查——一项治疗合并2型糖尿病的冠心病的随机研究（A Randomized Trial of Therapies for Type 2 Diabetes and Coronary Artery Disease，The BARI 2D Study Group，BARI 2D）的目的就是为了评价这类患者的治疗效果。
