Objective:To study the effect of the adjuvant Baogan Jiangzhi decoction therapy on liver function and insulin sensitivity in patients with nonalcoholic fatty liver disease.Methods: Patients who were newly diagnosed wi...Objective:To study the effect of the adjuvant Baogan Jiangzhi decoction therapy on liver function and insulin sensitivity in patients with nonalcoholic fatty liver disease.Methods: Patients who were newly diagnosed with nonalcoholic fatty liver disease in Nanchong Central Hospital and Suining Central Hospital between March 2015 and October 2017 were selected and randomly divided into the observation group who accepted Baogan Jiangzhi decoction combined with conventional western medicine treatment and the control group who accepted conventional western medicine treatment. The contents of liver damage indexes, inflammatory response markers and oxidative stress markers in serum as well as the expression of insulin signaling molecules in peripheral blood were measured before treatment and 2 weeks after treatment.Results:ALT, AST,γ-GT, TBIL, Chemerin, Wnt5a,β-catenin, MDA and NO contents in serum of both groups 2 weeks after treatment were lower than those before treatment whereas APN, Ghrelin, T-AOC and SOD contents in serum as well as IRS1, IRS2 and GLUT4 expression in peripheral blood were higher than those before treatment, and compared with those of control group, ALT, AST,γ-GT, TBIL, Chemerin, Wnt5a,β-catenin, MDA and NO contents in serum of observation group 2 weeks after treatment were lower whereas APN, Ghrelin, T-AOC and SOD contents in serum as well as IRS1, IRS2 and GLUT4 expression in peripheral blood were higher.Conclusion: Adjuvant Baogan Jiangzhi decoction therapy for nonalcoholic fatty liver disease can reduce liver function injury and improve insulin sensitivity.展开更多
The objective of this study was to investigate the clinical effect of combination treatment of Chinese medicine Baogan Lishui decoction and western medicine on hepatitis B(Hep B)cirrhosis-related refractory ascites.Th...The objective of this study was to investigate the clinical effect of combination treatment of Chinese medicine Baogan Lishui decoction and western medicine on hepatitis B(Hep B)cirrhosis-related refractory ascites.The control group was given conventional western medicine therapy while the observation group was given same conventional western medicine therapy with additional of Chinese medicine Baogan Lishui decoction.The total effective of clinical treatment on Hep B cirrhosis-related refractory ascites in observation group was higher than that of the control group which was 88.89%compared to 71.11%,respectively,and it was significant different(P<0.05).The combination treatment of Chinese medicine Baogan Lishui decoction and conventional western medicine can significantly increase the treatment effect on Hep B cirrhosis refractory ascites,and thus increases the quality and safety of life.展开更多
目的基于TLR4/NF-κB信号通路探讨枳葛保肝降脂方含药血清对乙醇诱导的人肝LO2细胞炎症损伤的作用及机制。方法将人肝LO2细胞分为正常组(10%正常大鼠血清)、诱导组(10%正常大鼠血清+2.5%无水乙醇)、美他多辛组(10%美他多辛含药血清+2.5...目的基于TLR4/NF-κB信号通路探讨枳葛保肝降脂方含药血清对乙醇诱导的人肝LO2细胞炎症损伤的作用及机制。方法将人肝LO2细胞分为正常组(10%正常大鼠血清)、诱导组(10%正常大鼠血清+2.5%无水乙醇)、美他多辛组(10%美他多辛含药血清+2.5%无水乙醇)和低、中、高浓度枳葛保肝降脂方含药血清组(简称“低、中、高浓度组”)(10.0%、5.0%、2.5%枳葛保肝降脂方含药血清+2.5%无水乙醇)。采用酶联免疫吸附试验法检测各组细胞裂解液中肿瘤坏死因子-α(TNF-α)、白细胞介素(IL)-1β、IL-18含量;Western blot法检测各组Toll样受体4(TLR4)、髓样分化因子88(MyD88)、NLR家族含pyrin结构域蛋白3(NLRP3)、Caspase-1、磷酸化核因子κB p65亚基/核因子κB p65亚基(p-NF-κBp65/NF-κBp65)蛋白表达水平;RT-qPCR检测各组TLR4、MYD88、NLRP3、Caspase-1 m RNA表达情况。结果与正常组比较,诱导组中TNF-α、IL-1β、IL-18含量,TLR4、My D88、NLRP3、Caspase-1、p-NF-κBp65/NF-κBp65蛋白表达水平,TLR4、MYD88、NLRP3、Caspase-1 m RNA表达量均升高(P<0.01)。与诱导组比较,中、高浓度组TNF-α、IL-1β、IL-18含量,TLR4、My D88、NLRP3、Caspase-1、p-NF-κBp65/NF-κBp65蛋白表达水平,TLR4、MYD88、NLRP3、Caspase-1、p-NF-κBp65/NF-κBp65mRNA表达量均降低(P<0.01)。结论枳葛保肝降脂方含药血清对无水乙醇诱导的人肝LO2细胞炎症损伤具有保护作用,其作用可能通过TLR4/NF-κB信号通路产生。展开更多
文摘Objective:To study the effect of the adjuvant Baogan Jiangzhi decoction therapy on liver function and insulin sensitivity in patients with nonalcoholic fatty liver disease.Methods: Patients who were newly diagnosed with nonalcoholic fatty liver disease in Nanchong Central Hospital and Suining Central Hospital between March 2015 and October 2017 were selected and randomly divided into the observation group who accepted Baogan Jiangzhi decoction combined with conventional western medicine treatment and the control group who accepted conventional western medicine treatment. The contents of liver damage indexes, inflammatory response markers and oxidative stress markers in serum as well as the expression of insulin signaling molecules in peripheral blood were measured before treatment and 2 weeks after treatment.Results:ALT, AST,γ-GT, TBIL, Chemerin, Wnt5a,β-catenin, MDA and NO contents in serum of both groups 2 weeks after treatment were lower than those before treatment whereas APN, Ghrelin, T-AOC and SOD contents in serum as well as IRS1, IRS2 and GLUT4 expression in peripheral blood were higher than those before treatment, and compared with those of control group, ALT, AST,γ-GT, TBIL, Chemerin, Wnt5a,β-catenin, MDA and NO contents in serum of observation group 2 weeks after treatment were lower whereas APN, Ghrelin, T-AOC and SOD contents in serum as well as IRS1, IRS2 and GLUT4 expression in peripheral blood were higher.Conclusion: Adjuvant Baogan Jiangzhi decoction therapy for nonalcoholic fatty liver disease can reduce liver function injury and improve insulin sensitivity.
文摘The objective of this study was to investigate the clinical effect of combination treatment of Chinese medicine Baogan Lishui decoction and western medicine on hepatitis B(Hep B)cirrhosis-related refractory ascites.The control group was given conventional western medicine therapy while the observation group was given same conventional western medicine therapy with additional of Chinese medicine Baogan Lishui decoction.The total effective of clinical treatment on Hep B cirrhosis-related refractory ascites in observation group was higher than that of the control group which was 88.89%compared to 71.11%,respectively,and it was significant different(P<0.05).The combination treatment of Chinese medicine Baogan Lishui decoction and conventional western medicine can significantly increase the treatment effect on Hep B cirrhosis refractory ascites,and thus increases the quality and safety of life.
文摘目的基于TLR4/NF-κB信号通路探讨枳葛保肝降脂方含药血清对乙醇诱导的人肝LO2细胞炎症损伤的作用及机制。方法将人肝LO2细胞分为正常组(10%正常大鼠血清)、诱导组(10%正常大鼠血清+2.5%无水乙醇)、美他多辛组(10%美他多辛含药血清+2.5%无水乙醇)和低、中、高浓度枳葛保肝降脂方含药血清组(简称“低、中、高浓度组”)(10.0%、5.0%、2.5%枳葛保肝降脂方含药血清+2.5%无水乙醇)。采用酶联免疫吸附试验法检测各组细胞裂解液中肿瘤坏死因子-α(TNF-α)、白细胞介素(IL)-1β、IL-18含量;Western blot法检测各组Toll样受体4(TLR4)、髓样分化因子88(MyD88)、NLR家族含pyrin结构域蛋白3(NLRP3)、Caspase-1、磷酸化核因子κB p65亚基/核因子κB p65亚基(p-NF-κBp65/NF-κBp65)蛋白表达水平;RT-qPCR检测各组TLR4、MYD88、NLRP3、Caspase-1 m RNA表达情况。结果与正常组比较,诱导组中TNF-α、IL-1β、IL-18含量,TLR4、My D88、NLRP3、Caspase-1、p-NF-κBp65/NF-κBp65蛋白表达水平,TLR4、MYD88、NLRP3、Caspase-1 m RNA表达量均升高(P<0.01)。与诱导组比较,中、高浓度组TNF-α、IL-1β、IL-18含量,TLR4、My D88、NLRP3、Caspase-1、p-NF-κBp65/NF-κBp65蛋白表达水平,TLR4、MYD88、NLRP3、Caspase-1、p-NF-κBp65/NF-κBp65mRNA表达量均降低(P<0.01)。结论枳葛保肝降脂方含药血清对无水乙醇诱导的人肝LO2细胞炎症损伤具有保护作用,其作用可能通过TLR4/NF-κB信号通路产生。
