BACKGROUND Patients with liver cirrhosis often experience muscle wasting that significantly affects their quality of life.AIM To investigate the effects of comprehensive nutritional and psychological interventions bas...BACKGROUND Patients with liver cirrhosis often experience muscle wasting that significantly affects their quality of life.AIM To investigate the effects of comprehensive nutritional and psychological interventions based on an amino acid-balanced diet on muscle wasting and quality of life in patients with liver cirrhosis.METHODS A retrospective study of 130 patients with liver cirrhosis(May 2023 to June 2024)was divided into control(conventional treatment,n=65)and observational groups(conventional+amino acid-balanced nutrition and psychological intervention,n=65).Anxiety(Self-Rating Anxiety Scale),depression(Self-Rating Depression Scale),lower limb muscle strength,and quality of life(Chronic Liver Disease Questionnaire)were assessed pre-intervention,and 3/6 months postintervention.RESULTS At baseline,no significant differences were observed between the two groups in terms of age,sex,etiology of cirrhosis,disease duration,disease severity,or any other measured outcome(P>0.05).At 3 months and 6 months post-intervention,the observational group exhibited significantly lower Self-Rating Anxiety Scale and Self-Rating Depression Scale scores than the control group(P<0.05),indicating reduced anxiety and depression as well as higher grip strength and increased lower limb muscle strength(P<0.05).Additionally,the observational group demonstrated superior Chronic Liver Disease Questionnaire scores across all domains, reflecting an improved quality of life (P < 0.05).CONCLUSIONAn amino acid-balanced diet and psychological intervention can effectively alleviate anxiety and depression andimprove muscle strength and quality of life in patients with cirrhosis and are worthy of clinical promotion.展开更多
Background Research on low-protein-level diets has indicated that even though the profiles of essential amino acids(EAAs)follow the recommendation for a normal-protein-level diet,broilers fed low-protein diets failed ...Background Research on low-protein-level diets has indicated that even though the profiles of essential amino acids(EAAs)follow the recommendation for a normal-protein-level diet,broilers fed low-protein diets failed to achieve pro-ductive performance compared to those fed normal diets.Therefore,it is imperative to reassess the optimum profile of EAAs in low-protein diets and establish a new ideal pattern for amino acid balance.Furthermore,identifying novel sensitive biomarkers for assessing amino acid balance will greatly facilitate the development of amino acid nutrition and application technology.In this study,12 dietary treatments[Con(+),Con(-),L&A(-),L&A(+),M&C(-),M&C(+),BCAA(-),BCAA(+),Thr(-),Thr(+),Trp(-)and Trp(+)]were established by combining different EAAs including lysine and argi-nine,methionine and cysteine,branched-chain amino acid(BCAA),threonine,and tryptophan to observe the growth and development of the broiler chickens fed with low-protein-level diets.Based on the biochemical parameters and untargeted metabolomic analysis of animals subjected to different treatments,biomarkers associated with opti-mal and suboptimal amino acid balance were identified.Results Growth performance,carcass characteristics,hepatic enzyme activity,serum biochemical parameters,and breast muscle mRNA expression differed significantly between male and female broilers under different dietary amino acid patterns.Male broilers exhibited higher sensitivity to the adjustment of amino acid patterns than female broilers.For the low-protein diet,the dietary concentrations of lysine,arginine,and tryptophan,but not of methionine,cystine,or threonine,needed to be increased.Therefore,further research on individual BCAA is required.For untar-geted metabolomic analysis,Con(+)was selected as a normal diet(NP)while Con(-)represented a low-protein diet(LP).L&A(+)denotes a low-protein amino acid balanced diet(LPAB)and Thr(+)represents a low-protein amino acid imbalance diet(LPAI).The metabolites oxypurinol,pantothenic acid,and D-octopine in birds were significantly influ-enced by different dietary amino acid patterns.Conclusion Adjusting the amino acid profile of low-protein diets is required to achieve normal growth performance in broiler chickens fed normal-protein diets.Oxypurinol,pantothenic acid,and D-octopine have been identified as potentially sensitive biomarkers for assessing amino acid balance.展开更多
The effects of crude protein（CP） levels in the diet on the m RNA expression of amino acid（AA） transporters were studied in a 45-d trial. Eighteen piglets with an initial body weight（BW） of 9.57 kg were assigned ...The effects of crude protein（CP） levels in the diet on the m RNA expression of amino acid（AA） transporters were studied in a 45-d trial. Eighteen piglets with an initial body weight（BW） of 9.57 kg were assigned to three groups（14%, 17%, and 20% CP in the diet） in a completely randomized design（six replicates per treatment）. Diets were supplemented with crystalline AA to achieve equal standardized ileal digestible contents of Lys, Met plus Cys, Thr, and Trp, and were provided ad libitum. After 45 d, all piglets were slaughtered to collect small intestine samples. Compared with the values in the 14% CP group, the expressions of ASCT2, 4F2 hc, and ATB0 m RNA in the jejunum were increased by 23.00%, 12.00%, 6.00% and 48.00%, 47.00%, 56.00% in the 17% and 20% CP groups, respectively. These results indicate that a 14% CP diet supplemented with crystalline AA may not transport enough AA into the body and maintain growth performance of piglets. However, a reduction of dietary 17% CP may reduce the excretion of nitrogen into the environment while supporting the development of piglets. Therefore, the 17% CP level is more suitable than 14% CP level.展开更多
The essential effect of vitamin A on immune function occurs through various mechanisms including direct effect on ThloTh2 balance modulation. However, it is unclear whether or not vitamin A can regulate Thl-Th2 balanc...The essential effect of vitamin A on immune function occurs through various mechanisms including direct effect on ThloTh2 balance modulation. However, it is unclear whether or not vitamin A can regulate Thl-Th2 balance under a strong Thl-polarizing condition. Therefore, the purpose of our study was to examine the effect of vitamin A metabolite allotrans retinoic acid （ATRA） on ThloTh2 differentiation in CD4~ T cells under GATA-3 deficiency, which can induce Thl-polarizing condition. In the present study, GATA-3 deficiency T cells were induced by siRNA and checked by real-time quantitative PCR and western blot. GATA-3 deficiency CD4＋ T cells and normal CD4＋ T were treated for 48 h with or without ATRA.展开更多
A new chemical index-imbalance degree (IBD), was proposed on the basis of cluster analysis in multivariate statistical analysis to estimate the extent of amino acid balance in protein. The range of IBD is between 0 an...A new chemical index-imbalance degree (IBD), was proposed on the basis of cluster analysis in multivariate statistical analysis to estimate the extent of amino acid balance in protein. The range of IBD is between 0 and 1, successively corresponding to complete balance and entire imbalance for the amino acid profile of a sample. The amino acid increment model of IBD belongs to an asymmetrical maximum-type, and there is a high correlation between IBD and coefficient of variation (CV). The nutritional value of individual feed can be ranked and clustered according to its IBD; and by calculating the IBD of amixed sample with two or more feedstuffs, it is Possible theoretically to predict whether a synergetic. pheno men on exists and when an optimal mutual complement can happen. If the conceptive frame of IBD extended, it can be used to study the balance of nutrients besides amino acids, and is helpful to realize the automatic distinction and selection in diet formulating.展开更多
Background：To compare the nutritional value and digestibility of five quality protein maize（QPM） hybrids to that of white and yellow maize,two experiments were carried out in growing pigs.In experiment 1,the energy...Background：To compare the nutritional value and digestibility of five quality protein maize（QPM） hybrids to that of white and yellow maize,two experiments were carried out in growing pigs.In experiment 1,the energy metabolizability and the nitrogen balance of growing pigs fed one of five QPM hybrid diets were compared against those of pigs fed white or yellow maize.In experiment 2,the apparent and standardized ileal digestibility（AID and SID,respectively） of proteins and amino acids from the five QPM hybrids were compared against those obtained from pigs fed white and yellow maize.In both experiments,the comparisons were conducted using contrasts.Results：The dry matter and nitrogen intakes were higher in the pigs fed the QPM hybrids（P 〈 0.05） than in the pigs fed white or yellow maize.Energy digestibility（P 〈 0.001） and metabolizability（P 〈 0.01） were higher in the pigs fed the white and yellow maize diets than in those fed the QPM diets.The AID of lysine was higher（P〈 0.01） in the QPM diets than in the white and yellow maize.The AIDs of leucine,isoleucine,valine,phenylalanine,and methionine were lower in the QPM diets than those of maize（white and yellow）（all P 〈 0.05）.Maize（white and yellow） had greater SIDs of leucine,isoleucine,valine,phenylalanine,glutamic acid,serine,alanine,tyrosine,and proline（P 〈 0.05）.Conclusions：Based on these results,it was concluded that QPM had a lower metabolizable energy content and a higher amount of digestible lysine than normal maize.展开更多
Objective:To explore the efficacy and safety of doxycycline and moxifloxacin in the treatment of tsutsugamushi disease.Methods:There was a total of 80 cases of tsutsugamushi disease that were treated in Jiangsu Sihong...Objective:To explore the efficacy and safety of doxycycline and moxifloxacin in the treatment of tsutsugamushi disease.Methods:There was a total of 80 cases of tsutsugamushi disease that were treated in Jiangsu Sihong Fenjinting Hospital from January 2017 to August 2020.The patients were divided into group A and group B,with 40 cases in each group.The patients in group A were treated with moxifloxacin whereas those in group B were treated with doxycycline.The efficacy and safety of the clinical treatment between the two groups were compared.Results:The effective rate was 72.5% in group A and 95.0% in group B.Compared with group A,group B was better(p<0.05).The time taken for the resolution of clinical symptoms,the detection indexes of liver function,and the incidence of adverse reactions were also compared between the two groups,in which group B was significantly better than group A(p<0.05).Conclusion:In the clinical treatment of tsutsugamushi disease,doxycycline has better therapeutic effect and higher safety compared to moxifloxacin.It can significantly improve the patienfs liver function,reduce the probability of adverse reactions,and accelerate the patienfs physical recovery.展开更多
文摘BACKGROUND Patients with liver cirrhosis often experience muscle wasting that significantly affects their quality of life.AIM To investigate the effects of comprehensive nutritional and psychological interventions based on an amino acid-balanced diet on muscle wasting and quality of life in patients with liver cirrhosis.METHODS A retrospective study of 130 patients with liver cirrhosis(May 2023 to June 2024)was divided into control(conventional treatment,n=65)and observational groups(conventional+amino acid-balanced nutrition and psychological intervention,n=65).Anxiety(Self-Rating Anxiety Scale),depression(Self-Rating Depression Scale),lower limb muscle strength,and quality of life(Chronic Liver Disease Questionnaire)were assessed pre-intervention,and 3/6 months postintervention.RESULTS At baseline,no significant differences were observed between the two groups in terms of age,sex,etiology of cirrhosis,disease duration,disease severity,or any other measured outcome(P>0.05).At 3 months and 6 months post-intervention,the observational group exhibited significantly lower Self-Rating Anxiety Scale and Self-Rating Depression Scale scores than the control group(P<0.05),indicating reduced anxiety and depression as well as higher grip strength and increased lower limb muscle strength(P<0.05).Additionally,the observational group demonstrated superior Chronic Liver Disease Questionnaire scores across all domains, reflecting an improved quality of life (P < 0.05).CONCLUSIONAn amino acid-balanced diet and psychological intervention can effectively alleviate anxiety and depression andimprove muscle strength and quality of life in patients with cirrhosis and are worthy of clinical promotion.
基金Shenyang Governmental Science and Technology Program(Project No.22-316-2-02)China Agriculture Research System Program(Project No.CARS-41-G04).
文摘Background Research on low-protein-level diets has indicated that even though the profiles of essential amino acids(EAAs)follow the recommendation for a normal-protein-level diet,broilers fed low-protein diets failed to achieve pro-ductive performance compared to those fed normal diets.Therefore,it is imperative to reassess the optimum profile of EAAs in low-protein diets and establish a new ideal pattern for amino acid balance.Furthermore,identifying novel sensitive biomarkers for assessing amino acid balance will greatly facilitate the development of amino acid nutrition and application technology.In this study,12 dietary treatments[Con(+),Con(-),L&A(-),L&A(+),M&C(-),M&C(+),BCAA(-),BCAA(+),Thr(-),Thr(+),Trp(-)and Trp(+)]were established by combining different EAAs including lysine and argi-nine,methionine and cysteine,branched-chain amino acid(BCAA),threonine,and tryptophan to observe the growth and development of the broiler chickens fed with low-protein-level diets.Based on the biochemical parameters and untargeted metabolomic analysis of animals subjected to different treatments,biomarkers associated with opti-mal and suboptimal amino acid balance were identified.Results Growth performance,carcass characteristics,hepatic enzyme activity,serum biochemical parameters,and breast muscle mRNA expression differed significantly between male and female broilers under different dietary amino acid patterns.Male broilers exhibited higher sensitivity to the adjustment of amino acid patterns than female broilers.For the low-protein diet,the dietary concentrations of lysine,arginine,and tryptophan,but not of methionine,cystine,or threonine,needed to be increased.Therefore,further research on individual BCAA is required.For untar-geted metabolomic analysis,Con(+)was selected as a normal diet(NP)while Con(-)represented a low-protein diet(LP).L&A(+)denotes a low-protein amino acid balanced diet(LPAB)and Thr(+)represents a low-protein amino acid imbalance diet(LPAI).The metabolites oxypurinol,pantothenic acid,and D-octopine in birds were significantly influ-enced by different dietary amino acid patterns.Conclusion Adjusting the amino acid profile of low-protein diets is required to achieve normal growth performance in broiler chickens fed normal-protein diets.Oxypurinol,pantothenic acid,and D-octopine have been identified as potentially sensitive biomarkers for assessing amino acid balance.
基金Project supported by the National Basic Research Program(973)of China(No.2013CB127301)the Natural Science Foundation of Hunan Province,China(No.12JJ2014)the National Natural Science Foundation of China(No.31402088)
文摘The effects of crude protein（CP） levels in the diet on the m RNA expression of amino acid（AA） transporters were studied in a 45-d trial. Eighteen piglets with an initial body weight（BW） of 9.57 kg were assigned to three groups（14%, 17%, and 20% CP in the diet） in a completely randomized design（six replicates per treatment）. Diets were supplemented with crystalline AA to achieve equal standardized ileal digestible contents of Lys, Met plus Cys, Thr, and Trp, and were provided ad libitum. After 45 d, all piglets were slaughtered to collect small intestine samples. Compared with the values in the 14% CP group, the expressions of ASCT2, 4F2 hc, and ATB0 m RNA in the jejunum were increased by 23.00%, 12.00%, 6.00% and 48.00%, 47.00%, 56.00% in the 17% and 20% CP groups, respectively. These results indicate that a 14% CP diet supplemented with crystalline AA may not transport enough AA into the body and maintain growth performance of piglets. However, a reduction of dietary 17% CP may reduce the excretion of nitrogen into the environment while supporting the development of piglets. Therefore, the 17% CP level is more suitable than 14% CP level.
基金supported by the National Natural Science Foundation of China(No.30671761)
文摘The essential effect of vitamin A on immune function occurs through various mechanisms including direct effect on ThloTh2 balance modulation. However, it is unclear whether or not vitamin A can regulate Thl-Th2 balance under a strong Thl-polarizing condition. Therefore, the purpose of our study was to examine the effect of vitamin A metabolite allotrans retinoic acid （ATRA） on ThloTh2 differentiation in CD4~ T cells under GATA-3 deficiency, which can induce Thl-polarizing condition. In the present study, GATA-3 deficiency T cells were induced by siRNA and checked by real-time quantitative PCR and western blot. GATA-3 deficiency CD4＋ T cells and normal CD4＋ T were treated for 48 h with or without ATRA.
文摘A new chemical index-imbalance degree (IBD), was proposed on the basis of cluster analysis in multivariate statistical analysis to estimate the extent of amino acid balance in protein. The range of IBD is between 0 and 1, successively corresponding to complete balance and entire imbalance for the amino acid profile of a sample. The amino acid increment model of IBD belongs to an asymmetrical maximum-type, and there is a high correlation between IBD and coefficient of variation (CV). The nutritional value of individual feed can be ranked and clustered according to its IBD; and by calculating the IBD of amixed sample with two or more feedstuffs, it is Possible theoretically to predict whether a synergetic. pheno men on exists and when an optimal mutual complement can happen. If the conceptive frame of IBD extended, it can be used to study the balance of nutrients besides amino acids, and is helpful to realize the automatic distinction and selection in diet formulating.
基金National Research Institute for Forestry,Crops and Livestock for the financial support given to this research project
文摘Background：To compare the nutritional value and digestibility of five quality protein maize（QPM） hybrids to that of white and yellow maize,two experiments were carried out in growing pigs.In experiment 1,the energy metabolizability and the nitrogen balance of growing pigs fed one of five QPM hybrid diets were compared against those of pigs fed white or yellow maize.In experiment 2,the apparent and standardized ileal digestibility（AID and SID,respectively） of proteins and amino acids from the five QPM hybrids were compared against those obtained from pigs fed white and yellow maize.In both experiments,the comparisons were conducted using contrasts.Results：The dry matter and nitrogen intakes were higher in the pigs fed the QPM hybrids（P 〈 0.05） than in the pigs fed white or yellow maize.Energy digestibility（P 〈 0.001） and metabolizability（P 〈 0.01） were higher in the pigs fed the white and yellow maize diets than in those fed the QPM diets.The AID of lysine was higher（P〈 0.01） in the QPM diets than in the white and yellow maize.The AIDs of leucine,isoleucine,valine,phenylalanine,and methionine were lower in the QPM diets than those of maize（white and yellow）（all P 〈 0.05）.Maize（white and yellow） had greater SIDs of leucine,isoleucine,valine,phenylalanine,glutamic acid,serine,alanine,tyrosine,and proline（P 〈 0.05）.Conclusions：Based on these results,it was concluded that QPM had a lower metabolizable energy content and a higher amount of digestible lysine than normal maize.
文摘Objective:To explore the efficacy and safety of doxycycline and moxifloxacin in the treatment of tsutsugamushi disease.Methods:There was a total of 80 cases of tsutsugamushi disease that were treated in Jiangsu Sihong Fenjinting Hospital from January 2017 to August 2020.The patients were divided into group A and group B,with 40 cases in each group.The patients in group A were treated with moxifloxacin whereas those in group B were treated with doxycycline.The efficacy and safety of the clinical treatment between the two groups were compared.Results:The effective rate was 72.5% in group A and 95.0% in group B.Compared with group A,group B was better(p<0.05).The time taken for the resolution of clinical symptoms,the detection indexes of liver function,and the incidence of adverse reactions were also compared between the two groups,in which group B was significantly better than group A(p<0.05).Conclusion:In the clinical treatment of tsutsugamushi disease,doxycycline has better therapeutic effect and higher safety compared to moxifloxacin.It can significantly improve the patienfs liver function,reduce the probability of adverse reactions,and accelerate the patienfs physical recovery.
