OBJECTIVE:To evaluate the efficacy of Baishao Luoshi decoction(白芍络石方,BD)on synaptic plasticity in rats with post stroke spasticity(PSS),and to study the mechanism behind the action.METHODS:The PSS model of rat wa...OBJECTIVE:To evaluate the efficacy of Baishao Luoshi decoction(白芍络石方,BD)on synaptic plasticity in rats with post stroke spasticity(PSS),and to study the mechanism behind the action.METHODS:The PSS model of rat was established by middle cerebral artery occlusion(MCAO).The neurological deficit symptoms were evaluated by modified neurological deficit score(mN SS).Muscle tension were evaluated by Modified Ashworth score(MAS).Transmission electron microscopy(TEM)was used to observe the synaptic ultrastructure.The expression of synaptic plasticity-related protein brain derived neurotrophic factor(BDNF),growth associated protein-43(GAP43),synaptophysin(p38)and microtubule-associated protein 2(MAP2)in the brain tissue around the infarct were detected by Western blotting.RESULTS:We found that mNSS were significantly improved and limb spasticity was ameliorated treated by BD.The thickness of postsynaptic density and the synaptic curvature increased significantly.The expression of synaptic plasticity-related protein BDNF,GAP43,p38,MAP2 in the brain tissue around the infarct were raised remarkably after treated by BD.CONCLUSIONS:Alleviating PSS by BD may be related to rescuing the synaptic plasticity,which provides a probable new therapeutic method for PSS.展开更多
基金Supported by the China Postdoctoral Science Foundation:Research on the Mechanism of Baishaoluoshi Decoction in Improving Poststroke Spasticity by KCC2-GABAAR Based on the Theory of“Gan Zhu Jin”(No.2020M682571)Natural Science Foundation of Hunan Province China:Research on the Mechanism of Post-stroke Spasticity by KCC2--GABAAR-Motoneuron Excitability and Chinese Traditional Medicine Intervention Based on the Theory of“Gan Zhu Jin”(No.2019JJ50343)the Scientific Research Project of Education Department of Hunan Province:Research on the Mechanism of Axonal Regeneration and TCM Intervention in Post-Stroke Spasticity Based on the Theory of“Gan Zhu Jin”by Slit-Robo Pathway(No.19C1423)。
文摘OBJECTIVE:To evaluate the efficacy of Baishao Luoshi decoction(白芍络石方,BD)on synaptic plasticity in rats with post stroke spasticity(PSS),and to study the mechanism behind the action.METHODS:The PSS model of rat was established by middle cerebral artery occlusion(MCAO).The neurological deficit symptoms were evaluated by modified neurological deficit score(mN SS).Muscle tension were evaluated by Modified Ashworth score(MAS).Transmission electron microscopy(TEM)was used to observe the synaptic ultrastructure.The expression of synaptic plasticity-related protein brain derived neurotrophic factor(BDNF),growth associated protein-43(GAP43),synaptophysin(p38)and microtubule-associated protein 2(MAP2)in the brain tissue around the infarct were detected by Western blotting.RESULTS:We found that mNSS were significantly improved and limb spasticity was ameliorated treated by BD.The thickness of postsynaptic density and the synaptic curvature increased significantly.The expression of synaptic plasticity-related protein BDNF,GAP43,p38,MAP2 in the brain tissue around the infarct were raised remarkably after treated by BD.CONCLUSIONS:Alleviating PSS by BD may be related to rescuing the synaptic plasticity,which provides a probable new therapeutic method for PSS.
