Objective:To investigate in vitro and in vivo antibacterial potentials of Vitex negundo(V. negundo) leaf extracts against diverse enteric pathogens.Methods:Water and methanol extracts of V.negundo leaves were evaluate...Objective:To investigate in vitro and in vivo antibacterial potentials of Vitex negundo(V. negundo) leaf extracts against diverse enteric pathogens.Methods:Water and methanol extracts of V.negundo leaves were evaluated against enteric bacterial pathogens by using standard disc diffusion,viable bacterial cell count methods,determination of minimum inhibitory concentrations(MIC) and minimum bactericidal concentrations(MBC).Results:Methanol extract of V.negundo leaves showed potent antibacterial activity(inhibition zone:9.9-22.6 mm,MIC: 200-3 200μg/mL.MBC:200-6 400μg/mL) against all the pathogenic enteric bacteria(Vibrio cholerae.Vibrio parahemolyticus.Vibrio mimicus.Echerichia coli,Shigella spps.,and Aeromonas spps) tested.Methanol extract of V.negundo leaves showed potent bactericidal activity both in vitro laboratory conditions(MBC,200-400μg/mL) and in the intestinal environment(Dose,1-2 mg/mL) of infant mice against pathogenic Vibrio cholerae,the major causative agent of cholera. Furthermore,assays using the mice cholera model showed that V.negundo methanol extract can protect mice from Vibrio cholerae infection and significandy decrease the mortality rate(P【0.0001). Conclusions:For the first time we showed thal medianol extract of V.negundo leaves exhibited strong vihriocidal activity both in vitro and in vivo conditions.Therefore,it will he useful to identify and isolate the active compounds of this extract that could be a good alternative of antibiotics to treat cholera.展开更多
To develop a novel food preservation technology for efficiently enhance bactericidal activity in a long term,hollow mesoporous silica spheres(HMSS)with regular nanostructures were applied to encapsulate natural organi...To develop a novel food preservation technology for efficiently enhance bactericidal activity in a long term,hollow mesoporous silica spheres(HMSS)with regular nanostructures were applied to encapsulate natural organic antimicrobial agents.The chemical structures,morphologies and thermal stabilities of linalool,HMSS and linalool-functionalized hollow mesoporous silica spheres(L-HMSS)nanoparticles were evaluated by polarimeter,field emission scanning electron microscope(FE-SEM),transmission electron microscope(TEM),fourier transform infrared(FT-IR),thermal gravimetric analyzer(TGA),nitrogen adsorption-desorption,zeta potential and small angle X-ray diffraction(SXRD).The results show that the linalool was successfully introduced into the cavities of HMSS,and the inorganic host exhibited a high loading capacity of about 1500 mg/g.In addition,after 48 h of incubatio n,the minimum bactericidal concentrations(MBC)of L-HMSS against Escherichia coli(E.coli),Salmonella enterica(S.enterica)and Staphylococcus aureus(S.aureus),Listeria monocytogenes(L.monocytogenes)were decreased to be 4(<5)mg/mL and 8(<10)mg/mL,respectively.These results revealed linaloolfunctionalized hollow mesoporous spheres could efficiently improve the bactericidal activities of the organic component.Furthermore,SEM images clearly showed that L-HMSS indeed had an extremely inhibitory effect against gram-negative(E.coli)and gram-positive(S.aureus)by breaking the structure of the cell membrane.This research is of great significance in the application of linalool in nano-delivery system as well as food industry.展开更多
Aims: The representativeness of the mandatory bacterial strains specified in European standards for in vitro assay of the bactericidal activity of antiseptics was evaluated by testing the activity of an antiseptic com...Aims: The representativeness of the mandatory bacterial strains specified in European standards for in vitro assay of the bactericidal activity of antiseptics was evaluated by testing the activity of an antiseptic combining chlorhexidine digluconate 0.2% and benzalkonium chloride 0.5% against 21 additional bacterial strains, and the positive interaction between these two biocidal agents was assessed. Methods and Results: The bactericidal activity of the antiseptic solution used pure or diluted was assessed according to the European standards EN 1040 and EN 13727. The contact time was 1 min at 20°C. Interfering substances used in the EN 13727 assay were bovine serum albumin and sheep erythrocytes, simulating “dirty” conditions, and hard water. A reduction of colony-forming units by ≥5 log10 was deemed to meet the requirements to conclude bactericidal activity. Under “basic” conditions, the bactericidal activity of the antiseptic was observed against all four mandatory strains specified in the standards as well as against nearly all the additional strains tested, including most of those with acquired antibiotic-resistance. The positive interaction between the two biocidal agents was also confirmed. Under “dirty” conditions, the bactericidal activity of the antiseptic solution was maintained against all the mandatory strains and was reduced against only four of the additional strains tested. Conclusions: With regard to the antiseptic tested and under the experimental conditions described, bactericidal activity evidenced against the mandatory strains appeared to be representative of that manifested against a wide range of the main pathogenic bacteria. Reduced bacterial activity against some of the additional strains tested (e.g. Enterobacteriaceae) was observed under “dirty” conditions. Significance and Impact of the Study: EN 13727 with some experimental adjustments represents an additional appropriate standard that needs to be considered for mucocutaneous antiseptic assessment. However, it may be worth including other specific bacterial strains to those specified in the standard, when evaluating antiseptics intended for use in certain clinical situations.展开更多
Objective:To investigate in vitro and in vivo antibacterial potentials of Vitex negundo(V. negundo) leaf extracts against diverse enteric pathogens.Methods:Water and methanol extracts of V.negundo leaves were evaluate...Objective:To investigate in vitro and in vivo antibacterial potentials of Vitex negundo(V. negundo) leaf extracts against diverse enteric pathogens.Methods:Water and methanol extracts of V.negundo leaves were evaluated against enteric bacterial pathogens by using standard disc diffusion,viable bacterial cell count methods,determination of minimum inhibitory concentrations(MIC) and minimum bactericidal concentrations(MBC).Results:Methanol extract of V.negundo leaves showed potent antibacterial activity(inhibition zone:9.9- 22.6 mm,MIC: 200-3 200μg/mL,MBC:200-6 400μg/mL) against all the pathogenic enleric bacteria(Vibrio cholerae,Vibrio parahaemolyticus,Vibrio mimicus,Echerichia coli.Shigella spps.,and Aeromonas spps) tested.Methanol extract of V.negundo leaves showed potent bactericidal activity both in vitro laboratory conditions(MBC,200-400μg/mL) and in the intestinal environment(Dose,1-2 mg/mL) of infant mice against pathogenic Vibrio cholerae,the major causative agent of cholera. Furthermore,assays using the mice cholera model showed that V.negundo methanol extract can protect mice from Vibrio cholerae infection and significantly decrease the mortality rate(P【0.0001).Conclusioas:For the first time we showed that methanol extract of V.negundo leaves exhibited strong vibriocidal activity both in vitro and in vivo conditions.Therefore,it will be useful to identify and isolate the active compounds of this extract that could be a good alternative of antibiotics to treat cholera.展开更多
Pure TiO2 thin films and iron doped TiO2 thin films on glass substrate were prepared by sol-gel method, and characterized by X-ray diffractometer （XRD）, thermo-gravimetric analysis （TG-DSC）, high resolution transm...Pure TiO2 thin films and iron doped TiO2 thin films on glass substrate were prepared by sol-gel method, and characterized by X-ray diffractometer （XRD）, thermo-gravimetric analysis （TG-DSC）, high resolution transmission electron microscope （HRTEM）, scanning electron microscope （SEM） and UV-Vis spectroscopy, respectively. The experimental results show that the pure TiO2 thin films and iron doped TiO2 thin films can destroy most of the escherichia coli and bacillus subtillis under the irradiation of 365 nm UV-light. However, the iron doped TiO2 thin film is a better photocatalyst than pure TiO2 thin film. The ultrastructural studies provide direct evidences for understanding the bactericidal mechanism of the TiO2 photocatalyst.展开更多
Polysaccharide-based composite materials（beads） containing silver nanoparticles（AgNPs） were successfully prepared.Hydrogel beads acted as an efficient vehicle for Ag＊ delivery.Beads promoted the AgNPs protection ...Polysaccharide-based composite materials（beads） containing silver nanoparticles（AgNPs） were successfully prepared.Hydrogel beads acted as an efficient vehicle for Ag＊ delivery.Beads promoted the AgNPs protection and inhibited their aggregation.Antimicrobial assays showed that the beads/AgNPs concentration can be modulated to deliver an amount of Ag＊ necessary for kill Escherichia coli cells.展开更多
Introduction In the preceding paper it was described that various 1-acyl-4-substituted thiosemicarbazides and their derivatives exhibited a broad spectrum of antibacterial activities. Kothari pointed out that some het...Introduction In the preceding paper it was described that various 1-acyl-4-substituted thiosemicarbazides and their derivatives exhibited a broad spectrum of antibacterial activities. Kothari pointed out that some heterocycles linked with tetrazole and 1,2,4-triazole can be used as antiinflammatory agent. We have also demonstrated that both 3-(5-a- naphthyl)-2H-tetrazol-2-ylmethyl)-4-aryl-1,2,4-triazole-5-thiones and 3-(3-pyridyl)-展开更多
This paper reports the preparation of three di‑iron complexes containing a thiazole moiety.Esterification of complex[Fe_(2)(CO)_(6)(μ‑SCH_(2)CH(CH_(2)OH)S)](1)with 4‑methylthiazole‑5‑carboxylic acid gave the correspo...This paper reports the preparation of three di‑iron complexes containing a thiazole moiety.Esterification of complex[Fe_(2)(CO)_(6)(μ‑SCH_(2)CH(CH_(2)OH)S)](1)with 4‑methylthiazole‑5‑carboxylic acid gave the corresponding ester[Fe_(2)(CO)_(6)(μ‑tedt)](2),where tedt=SCH_(2)CH(CH_(2)OOC(5‑C_(3)HNSCH_(3)))S.Further reactions of complex 2 with tri(ptolyl)phosphine(tp)or tris(4‑fluorophenyl)phosphine(fp)gave the phosphine‑substituted derivatives[Fe_(2)(CO)_(5)(tp)(μ‑tedt)](3)and[Fe_(2)(CO)_(5)(fp)(μ‑tedt)](4).The structures of the newly prepared complexes were elucidated by elemental analysis,NMR,IR,and X‑ray photoelectron spectroscopy.Moreover,single‑crystal X‑ray diffraction analysis confirmed their molecular structures,showing that they contain a di‑iron core ligated by a bridged dithiolate bearing a thiazole moiety and terminal carbonyls.The electrochemical and electrocatalytic proton reduction were probed by cyclic voltammetry,revealing that three complexes can catalyze the reduction of protons to H_(2) under the electrochemical conditions.For comparison,complex 4 possessed the best efficiency with a turnover frequency of 23.5 s^(-1)at 10 mmol·L^(-1)HOAc concentration.In addition,the fungicidal activity of these complexes was also investigated in this study.CCDC:2477511,2;2477512,3;2477513,4.展开更多
Chitosan(CTS)was grafted onto the surface of amino‑functionalized silver chloride silicon dioxide(AgCl@SiO_(2)‑NH_(2))cores to obtain AgCl@SiO_(2)/CTS hybrid nanoparticles.The as‑obtained AgCl@SiO_(2)/CTS nanoparticle...Chitosan(CTS)was grafted onto the surface of amino‑functionalized silver chloride silicon dioxide(AgCl@SiO_(2)‑NH_(2))cores to obtain AgCl@SiO_(2)/CTS hybrid nanoparticles.The as‑obtained AgCl@SiO_(2)/CTS nanoparticles were chlorinated by NaClO solution to get AgCl@SiO_(2)/CTS‑based chloramine nano‑hybrid materials,denoted as AgCl@SiO_(2)/CTS‑Cl.A transmission electron microscope was used to observe the morphology of the as‑prepared samples AgCl@SiO_(2)/CTS and AgCl@SiO_(2)/CTS‑Cl.At the same time,an X‑ray diffractometer and an infrared spectroscope were utilized to characterize their crystal and chemical structures.Besides,ζpotentials were measured to elucidate the surface modification of AgCl nanoparticles by—NH_(2),the antibacterial mechanism of AgCl@SiO_(2)/CTS‑Cl was investigated by scanning electron microscopy,and Escherichia coli(E.coli)and Staphylococcus aureus(S.aureus)were used as the to‑be‑tested strains to evaluate the antimicrobial activity of samples AgCl@SiO_(2)/CTS and AgCl@SiO_(2)/CTS‑Cl.Findings demonstrate that sample AgCl@SiO_(2)/CTS exhibits a chain‑like structure ascribed to the interaction between—NH_(2),and each AgCl@SiO_(2)/CTS hybrid nanoparticle contains several AgCl cores.In the meantime,sample AgCl@SiO_(2)/CTS‑Cl exhibits excellent antibacterial activity against E.coli and S.aureus,which is attributed to the synergistic antibacterial effect of Ag^(+)and Cl^(-).Sample AgCl@SiO_(2)/CTS‑Cl with a dosage of 640.00μg·mL^(-1) could completely kill the two kinds of tested bacteria in 12 h of incubation;it retains a high antibacterial efficiency even after 10 cycles of antibacterial tests.展开更多
BACKGROUND Postoperative delirium(POD)is a common and serious complication in surgical patients,particularly older adults.Alterations in cholinergic function have been implicated in its pathophysiology.AIM To evaluate...BACKGROUND Postoperative delirium(POD)is a common and serious complication in surgical patients,particularly older adults.Alterations in cholinergic function have been implicated in its pathophysiology.AIM To evaluate the association between preoperative serum cholinesterase(ChE)activity—specifically butyrylcholinesterase(BuChE)and acetylcholinesterase(AChE)—and the risk of POD in adult surgical patients in a meta-analysis.METHODS A systematic search was conducted in PubMed,EMBASE,and Web of Science up to March 28,2025 for studies reporting preoperative serum BuChE or AChE activity in relation to subsequent POD incidence.Standardized mean differences(SMDs)and odds ratios(ORs)with 95%confidence intervals(CIs)were pooled using random-effects models.Subgroup and sensitivity analyses were performed based on follow-up duration and analytic models.RESULTS Thirteen studies(n=2730 patients)were included.Patients who developed POD had significantly lower preoperative BuChE activity than those who did not(SMD=-0.28;95%CI:-0.39 to-0.16;I^(2)=18%).Higher BuChE activity was associated with a reduced risk of POD(OR per 100 unit increment=0.97;95%CI:0.95-0.99;I2=0%).In contrast,pooled AChE activity did not differ significantly between POD and non-POD groups(SMD=-0.25;95%CI:-0.53 to 0.03;P=0.08;I^(2)=80%),and the ORs per 1 unit increment in AChE activity were not statistically significant(OR=0.98;95%CI:0.95-1.01).CONCLUSION Lower preoperative serum BuChE activity is associated with an increased risk of POD in adults undergoing surgery.BuChE activity may serve as a potential preoperative biomarker for POD risk stratification.展开更多
[Objectives]This study was conducted to investigate the extraction process,content determination,and antioxidant properties of flavonoids from Hylocereus undatus(Haw.)Britton&Rose.[Methods]Using H.undatus as the r...[Objectives]This study was conducted to investigate the extraction process,content determination,and antioxidant properties of flavonoids from Hylocereus undatus(Haw.)Britton&Rose.[Methods]Using H.undatus as the raw material,the effects of ethanol concentration,ultrasonic temperature,time,and solid-to-liquid ratio on the total flavonoid yield were investigated through single-factor and orthogonal experiments.[Results]All factors had a significant effect on the yield.The optimized conditions were determined as follows:ethanol concentration 75%,ultrasonic temperature 60℃,ultrasonic time 30 min,and solid-to-liquid ratio 1:50(g/ml).Under these conditions,the total flavonoid yield reached 3.08%.Evaluation of antioxidant activity revealed that the extract exhibited superior scavenging rates against both DPPH and hydroxyl radicals compared with the standard reference compound BHT.[Conclusions]This study holds significant importance for elucidating the pharmacological mechanisms of flavonoids in H.undatus and for expanding their application in medicine and health products.展开更多
Overweight and obesity are significant public health concerns worldwide due to their association with many chronic health conditions.This has resulted in the development of various interventions focused on weight loss...Overweight and obesity are significant public health concerns worldwide due to their association with many chronic health conditions.This has resulted in the development of various interventions focused on weight loss to reduce the associated health burden.Physical activity is an important lifestyle behavior associated with enhanced health.Evidence supports that many of the benefits of physical activity are realized independent of initial weight status or whether weight loss is achieved,with some benefits additive to what is achieved with weight loss alone.These benefits include enhanced cardiometabolic,brain,cognitive and psychological health,and others.Moreover,in adults with overweight or obesity,physical activity has independent effects on cardiorespiratory fitness,muscular strength,physical function,and mobility.There are also benefits to body composition,with physical activity improving the quality of key tissues,such as skeletal muscle,which may not occur with diet-induced weight loss.Therefore,physical activity is an important public health target for adults with overweight or obesity to provide a wide range of health benefits that extend beyond those of weight loss alone.However,physical activity recommendations and programming efforts should consider the unique characteristics of adults with overweight or obesity to be most effective,and should support a focus on mobility,physical function,and other health outcomes.展开更多
Conventional ultrasound(US)evaluation of enthesitis in psoriatic arthritis(PsA)is limited by its inability to quantify metabolic alterations such as hypoxia,a key driver of disease activity.We introduce an oxygenation...Conventional ultrasound(US)evaluation of enthesitis in psoriatic arthritis(PsA)is limited by its inability to quantify metabolic alterations such as hypoxia,a key driver of disease activity.We introduce an oxygenation-integrated multimodal photoacoustic/ultrasound(PA/US)imaging framework designed to quantify entheseal oxygen saturation(SO_(2))for assessing entheseal disease activity in PsA.In this cross-sectional study,25 PsA patients underwent bilateral PA/US imaging of 12 entheses,where ultrasound lesions were scored using the Outcome Measures in Rheumatology scoring system,and PA-derived SO_(2) levels,quantified via dual-wavelength PA imaging,were classified into hyperoxia or hypoxia groups using k-means clustering.This approach provides metabolic insights complementary to conventional ultrasonic assessment.A composite score integrating hypoxia with US parameters was validated against clinical disease activity indices(Disease Activity Score 28-C-reactive protein,DAS28-CRP;Disease Activity Index for Psoriatic Arthritis,DAPSA).Among 300 entheses,103(34.3%)exhibited PA positivity,with 40(38.8%)classified as hypoxia.Hypoxia scores independently predicted DAS28-CRP(β=0.618,p=0.001)and DAPSA(β=0.612,p<0:001).The hypoxia-optimized PAUS score demonstrated superior correlation with disease activity indices compared to conventional US(DAS28-CRP:r=0.615,p=0.001 versus r=0.474,p=0.017;DAPSA:r=0.743,p<0:001 versus r=0.567,p=0.003),alongside superior diagnostic accuracy for minimal disease activity(area under the curve,AUC 0.776 versus 0.614,p=0.008)and low disease activity(AUC 0.853 versus 0.772,p=0.009).This multimodal scoring system enhances the stratification of PsA disease activity by providing unique metabolic insights,offering a potential tool for therapeutic monitoring and guiding treat-to-target strategies.展开更多
BACKGROUND Major depressive disorder(MDD)and obesity(OB)are bidirectionally comorbid conditions with common neurobiological underpinnings.However,the neurocognitive mechanisms of their comorbidity remain poorly unders...BACKGROUND Major depressive disorder(MDD)and obesity(OB)are bidirectionally comorbid conditions with common neurobiological underpinnings.However,the neurocognitive mechanisms of their comorbidity remain poorly understood.AIM To examine regional abnormalities in spontaneous brain activity among patients with MDD-OB comorbidity.METHODS This study adopted a regional homogeneity(ReHo)analysis of resting-state functional magnetic resonance imaging.The study included 149 hospital patients divided into four groups:Patients experiencing their first episode of drug-naive MDD with OB,patients with MDD without OB,and age-and sex-matched healthy individuals with and without OB.Whole-brain ReHo analysis was conducted using SPM12 software and RESTplus toolkits,with group comparisons via ANOVA and post-hoc tests.Correlations between ReHo values and behavioral measures were examined.RESULTS ANOVA revealed significant whole-brain ReHo differences among the four groups in four key regions:The left middle temporal gyrus(MTG.L),right cuneus,left precuneus,and left thalamus.Post-hoc analyses confirmed pairwise differences between all groups across these regions(P<0.05).OB was associated with ReHo alterations in the MTG.L,right cuneus,and left thalamus,whereas abnormalities in the precuneus suggested synergistic pathological mechanisms between MDD and OB.Statistically significant correlations were found between the drive and fun-seeking dimensions of the behavioral activation system,as well as behavioral inhibition and the corresponding ReHo values.CONCLUSION Our findings provide novel evidence for the neuroadaptive mechanisms underlying the MDD-OB comorbidity.Further validation could lead to personalized interventions targeting MTG.L hyperactivity and targeting healthy food cues.展开更多
The photothermal effect enhances the antibacterial activity by generating localized heat that disrupts microbial cells,leading to increased bacterial inactivation.The objective of this study was to develop and evaluat...The photothermal effect enhances the antibacterial activity by generating localized heat that disrupts microbial cells,leading to increased bacterial inactivation.The objective of this study was to develop and evaluate the antibacterial and photothermal applications of polydopamine-berberine nanoparticles(PDA-BBR-NPs),incorporating them into a sodium alginate matrix for food preservation.The self-assembled PDA-BBR-NPs were synthesized using a solution oxidation method and characterized by scanning electron microscopy,Fourier transform infrared spectroscopy,and nanoparticle size analysis.The results demonstrated that PDA-BBR-NPs were uniform spheres with an average diameter of 177.1 nm.Drug loading and encapsulation efficiency tests showed that the optimal formulation had a drug loading capacity of 48.4%and an encapsulation efficiency of 84.70%.Antibacterial activity against Escherichia coli and Staphylococcus aureus was evaluated using the dilution coating plate method,showing an inhibition rate of over 98%.Photothermal testing under 808 nm near-infrared irradiation revealed a maximum photothermal conversion efficiency of 32.65%.To assess the practical application of the nanoparticles,they were incorporated into a sodium alginate-based coating for the preservation of Kyoho grapes.Key parameters such as weight loss rate,pH,titratable acidity,and soluble solids content were monitored over a 12-day storage period at room temperature.The PDA-BBR-near-infrared spectroscopy-treated group exhibited significantly reduced weight loss rate(6.08%lower than the control group)and a slower decrease in pH.The coating effectively inhibited microbial activity and extended the shelf life of the grapes by 3-6 days compared to the control group.These findings demonstrated the potential of PDA-BBR-NPs to enhance the preservation of fresh produce through their combined antibacterial and photothermal effects,offering a promising method for extending fruit shelf life.展开更多
Background:Early detection of harmful brain activity in critically ill patients using electroencephalography(EEG)is vital for timely and effective clinical intervention.Automating EEG analysis with deep learning techn...Background:Early detection of harmful brain activity in critically ill patients using electroencephalography(EEG)is vital for timely and effective clinical intervention.Automating EEG analysis with deep learning techniques holds significant promise for enhancing diagnostic efficiency and accuracy.Methods:We implemented EfficientNetB2,which leverages convolutional neural networks with a novel Temporal Squeeze-and-Excitation module to capture temporal EEG features,and WaveNet,a sequential model designed to effectively model temporal dependencies in EEG data using dilated causal convolutions and temporal self-attention.Both models were trained and evaluated using a publicly available EEG dataset,with performance assessed via 4-fold cross-validation and a step-wise learning rate reduction strategy.Results:Our results demonstrate a significant reduction in training loss from 0.6459 to 0.3055 and validation loss from 0.9602 to 0.5719 over six epochs.Consistent improvements were observed across cross-validation folds,highlighting the robustness of the models.Additionally,ensemble learning of the two architectures further enhanced classification performance.Conclusion:This comparative analysis sheds light on the strengths and limitations of EfficientNetB2 and WaveNet for automated harmful brain activity detection in EEG signals.The findings contribute to the advancement of reliable and efficient deep learning models,paving the way for their clinical application in managing critically ill patients.展开更多
Aberrant activation of Receptor Tyrosine Kinases(RTKs)is a well-established trigger of tumorigenesis,and the over-use of RTK inhibitors often leads to drug resistance and tumor recurrence.While current Drug-Target Int...Aberrant activation of Receptor Tyrosine Kinases(RTKs)is a well-established trigger of tumorigenesis,and the over-use of RTK inhibitors often leads to drug resistance and tumor recurrence.While current Drug-Target Interaction(DTI)prediction methods(including those based on heterogeneous information networks)have shown promise,they remain limited in their ability to fully capture the nature of DTIs and often lack interpretability.To overcome these limitations,this study introduces a novel hybrid optimization model termed MDBO-RF,which integrates a Modified Dung Beetle Optimizer(MDBO)with Random Forest(RF).The key innovation lies in the enhancement of the DBO algorithm through a quaternion-based learning mechanism and the Cauchy mutation strategy,specifically designed to overcome the slow convergence and susceptibility to local optima that plague traditional metaheuristic algorithms used for hyperparameter tuning.The model leverages commonly used molecular descriptors to enhance the prediction of Tyrosine Kinase(TK)inhibitory activity and enable efficient compound screening.Our results demonstrate that MDBO-RF achieves a 3.41%increase in prediction accuracy compared to the standard RF model and outperforms several other contemporary machine learning approaches.The model effectively streamlines the RTK inhibitor screening process by improving prediction accuracy in multi-target competitive binding scenarios and reducing false-positive screening due to off-target effects.This work underscores the value of hybrid optimization strategies in bioinformatics and provides a robust,interpretable tool for accelerating drug discovery.展开更多
Human Activity Recognition(HAR)is a novel area for computer vision.It has a great impact on healthcare,smart environments,and surveillance while is able to automatically detect human behavior.It plays a vital role in ...Human Activity Recognition(HAR)is a novel area for computer vision.It has a great impact on healthcare,smart environments,and surveillance while is able to automatically detect human behavior.It plays a vital role in many applications,such as smart home,healthcare,human computer interaction,sports analysis,and especially,intelligent surveillance.In this paper,we propose a robust and efficient HAR system by leveraging deep learning paradigms,including pre-trained models,CNN architectures,and their average-weighted fusion.However,due to the diversity of human actions and various environmental influences,as well as a lack of data and resources,achieving high recognition accuracy remain elusive.In this work,a weighted average ensemble technique is employed to fuse three deep learning models:EfficientNet,ResNet50,and a custom CNN.The results of this study indicate that using a weighted average ensemble strategy for developing more effective HAR models may be a promising idea for detection and classification of human activities.Experiments by using the benchmark dataset proved that the proposed weighted ensemble approach outperformed existing approaches in terms of accuracy and other key performance measures.The combined average-weighted ensemble of pre-trained and CNN models obtained an accuracy of 98%,compared to 97%,96%,and 95%for the customized CNN,EfficientNet,and ResNet50 models,respectively.展开更多
The Almus Fault Zone(AFZ)is one of the major splay faults of the North Anatolian Fault Zone(NAFZ)and is important for understanding its tectonic features and assessing regional seismic hazards.This research presents t...The Almus Fault Zone(AFZ)is one of the major splay faults of the North Anatolian Fault Zone(NAFZ)and is important for understanding its tectonic features and assessing regional seismic hazards.This research presents the integration of morphometric indices to quantitatively assess the spatial variation of tectonic activity along the AFZ.The AFZ is an active fault with both strike-slip and normal fault components and consists of two main branches,Mercimekdağı-Çamdere Fault(MÇF)and Tokat Fault(TF)segments.This study aims to assess the relative tectonic activity of the AFZ using various morphometric indices,based on a 10 m resolution DEM,with the aid of ArcGIS and MATLAB software.For this purpose,morphometric indices such as hypsometric integral(HI:0.35-0.65),mountain front sinuosity(Smf:1.3-1.44),valley floor width-height ratio(Vf:0.15-2.28),asymmetry factor(AF:23-77),drainage basin shape(Bs:1.13-6.10)and normalized steepness index(ksn:1-498)were applied to 53 drainage basins.When the Smf and mean Vf indices results were evaluated,it was calculated that the uplift ratio of the region was more than 0.5 mm/yr.The spatial distribution of the relative tectonic activity(Iat)of the area was revealed by combining the obtained morphometric indices analysis results.According to the Iat result,it was concluded that the MercimekdağıÇamdere Fault and Tokat Fault segments have high tectonic activity,but the Mercimekdağı-Çamdere Fault segment has higher tectonic activity.The results obtained were also confirmed by field observations.This research provides valuable information for the evaluation of tectonic activity in drainage systems controlled by splay faults.展开更多
文摘Objective:To investigate in vitro and in vivo antibacterial potentials of Vitex negundo(V. negundo) leaf extracts against diverse enteric pathogens.Methods:Water and methanol extracts of V.negundo leaves were evaluated against enteric bacterial pathogens by using standard disc diffusion,viable bacterial cell count methods,determination of minimum inhibitory concentrations(MIC) and minimum bactericidal concentrations(MBC).Results:Methanol extract of V.negundo leaves showed potent antibacterial activity(inhibition zone:9.9-22.6 mm,MIC: 200-3 200μg/mL.MBC:200-6 400μg/mL) against all the pathogenic enteric bacteria(Vibrio cholerae.Vibrio parahemolyticus.Vibrio mimicus.Echerichia coli,Shigella spps.,and Aeromonas spps) tested.Methanol extract of V.negundo leaves showed potent bactericidal activity both in vitro laboratory conditions(MBC,200-400μg/mL) and in the intestinal environment(Dose,1-2 mg/mL) of infant mice against pathogenic Vibrio cholerae,the major causative agent of cholera. Furthermore,assays using the mice cholera model showed that V.negundo methanol extract can protect mice from Vibrio cholerae infection and significandy decrease the mortality rate(P【0.0001). Conclusions:For the first time we showed thal medianol extract of V.negundo leaves exhibited strong vihriocidal activity both in vitro and in vivo conditions.Therefore,it will he useful to identify and isolate the active compounds of this extract that could be a good alternative of antibiotics to treat cholera.
基金The National Natural Science Foundation of China(No.31701678)the Shanghai Key Research Projects of Promoting Agriculture by Science and Technology(No.2019-02-08-00-15-F01147)supported this work。
文摘To develop a novel food preservation technology for efficiently enhance bactericidal activity in a long term,hollow mesoporous silica spheres(HMSS)with regular nanostructures were applied to encapsulate natural organic antimicrobial agents.The chemical structures,morphologies and thermal stabilities of linalool,HMSS and linalool-functionalized hollow mesoporous silica spheres(L-HMSS)nanoparticles were evaluated by polarimeter,field emission scanning electron microscope(FE-SEM),transmission electron microscope(TEM),fourier transform infrared(FT-IR),thermal gravimetric analyzer(TGA),nitrogen adsorption-desorption,zeta potential and small angle X-ray diffraction(SXRD).The results show that the linalool was successfully introduced into the cavities of HMSS,and the inorganic host exhibited a high loading capacity of about 1500 mg/g.In addition,after 48 h of incubatio n,the minimum bactericidal concentrations(MBC)of L-HMSS against Escherichia coli(E.coli),Salmonella enterica(S.enterica)and Staphylococcus aureus(S.aureus),Listeria monocytogenes(L.monocytogenes)were decreased to be 4(<5)mg/mL and 8(<10)mg/mL,respectively.These results revealed linaloolfunctionalized hollow mesoporous spheres could efficiently improve the bactericidal activities of the organic component.Furthermore,SEM images clearly showed that L-HMSS indeed had an extremely inhibitory effect against gram-negative(E.coli)and gram-positive(S.aureus)by breaking the structure of the cell membrane.This research is of great significance in the application of linalool in nano-delivery system as well as food industry.
文摘Aims: The representativeness of the mandatory bacterial strains specified in European standards for in vitro assay of the bactericidal activity of antiseptics was evaluated by testing the activity of an antiseptic combining chlorhexidine digluconate 0.2% and benzalkonium chloride 0.5% against 21 additional bacterial strains, and the positive interaction between these two biocidal agents was assessed. Methods and Results: The bactericidal activity of the antiseptic solution used pure or diluted was assessed according to the European standards EN 1040 and EN 13727. The contact time was 1 min at 20°C. Interfering substances used in the EN 13727 assay were bovine serum albumin and sheep erythrocytes, simulating “dirty” conditions, and hard water. A reduction of colony-forming units by ≥5 log10 was deemed to meet the requirements to conclude bactericidal activity. Under “basic” conditions, the bactericidal activity of the antiseptic was observed against all four mandatory strains specified in the standards as well as against nearly all the additional strains tested, including most of those with acquired antibiotic-resistance. The positive interaction between the two biocidal agents was also confirmed. Under “dirty” conditions, the bactericidal activity of the antiseptic solution was maintained against all the mandatory strains and was reduced against only four of the additional strains tested. Conclusions: With regard to the antiseptic tested and under the experimental conditions described, bactericidal activity evidenced against the mandatory strains appeared to be representative of that manifested against a wide range of the main pathogenic bacteria. Reduced bacterial activity against some of the additional strains tested (e.g. Enterobacteriaceae) was observed under “dirty” conditions. Significance and Impact of the Study: EN 13727 with some experimental adjustments represents an additional appropriate standard that needs to be considered for mucocutaneous antiseptic assessment. However, it may be worth including other specific bacterial strains to those specified in the standard, when evaluating antiseptics intended for use in certain clinical situations.
文摘Objective:To investigate in vitro and in vivo antibacterial potentials of Vitex negundo(V. negundo) leaf extracts against diverse enteric pathogens.Methods:Water and methanol extracts of V.negundo leaves were evaluated against enteric bacterial pathogens by using standard disc diffusion,viable bacterial cell count methods,determination of minimum inhibitory concentrations(MIC) and minimum bactericidal concentrations(MBC).Results:Methanol extract of V.negundo leaves showed potent antibacterial activity(inhibition zone:9.9- 22.6 mm,MIC: 200-3 200μg/mL,MBC:200-6 400μg/mL) against all the pathogenic enleric bacteria(Vibrio cholerae,Vibrio parahaemolyticus,Vibrio mimicus,Echerichia coli.Shigella spps.,and Aeromonas spps) tested.Methanol extract of V.negundo leaves showed potent bactericidal activity both in vitro laboratory conditions(MBC,200-400μg/mL) and in the intestinal environment(Dose,1-2 mg/mL) of infant mice against pathogenic Vibrio cholerae,the major causative agent of cholera. Furthermore,assays using the mice cholera model showed that V.negundo methanol extract can protect mice from Vibrio cholerae infection and significantly decrease the mortality rate(P【0.0001).Conclusioas:For the first time we showed that methanol extract of V.negundo leaves exhibited strong vibriocidal activity both in vitro and in vivo conditions.Therefore,it will be useful to identify and isolate the active compounds of this extract that could be a good alternative of antibiotics to treat cholera.
基金the National"973"Plan Research Project(No.2004CB619204)Educational Ministry Scientific and Technological Research Key Project(No.02052)
文摘Pure TiO2 thin films and iron doped TiO2 thin films on glass substrate were prepared by sol-gel method, and characterized by X-ray diffractometer （XRD）, thermo-gravimetric analysis （TG-DSC）, high resolution transmission electron microscope （HRTEM）, scanning electron microscope （SEM） and UV-Vis spectroscopy, respectively. The experimental results show that the pure TiO2 thin films and iron doped TiO2 thin films can destroy most of the escherichia coli and bacillus subtillis under the irradiation of 365 nm UV-light. However, the iron doped TiO2 thin film is a better photocatalyst than pure TiO2 thin film. The ultrastructural studies provide direct evidences for understanding the bactericidal mechanism of the TiO2 photocatalyst.
基金the financial support (Nos.481424/2010-5 and 308337/2013-1)
文摘Polysaccharide-based composite materials（beads） containing silver nanoparticles（AgNPs） were successfully prepared.Hydrogel beads acted as an efficient vehicle for Ag＊ delivery.Beads promoted the AgNPs protection and inhibited their aggregation.Antimicrobial assays showed that the beads/AgNPs concentration can be modulated to deliver an amount of Ag＊ necessary for kill Escherichia coli cells.
文摘Introduction In the preceding paper it was described that various 1-acyl-4-substituted thiosemicarbazides and their derivatives exhibited a broad spectrum of antibacterial activities. Kothari pointed out that some heterocycles linked with tetrazole and 1,2,4-triazole can be used as antiinflammatory agent. We have also demonstrated that both 3-(5-a- naphthyl)-2H-tetrazol-2-ylmethyl)-4-aryl-1,2,4-triazole-5-thiones and 3-(3-pyridyl)-
文摘This paper reports the preparation of three di‑iron complexes containing a thiazole moiety.Esterification of complex[Fe_(2)(CO)_(6)(μ‑SCH_(2)CH(CH_(2)OH)S)](1)with 4‑methylthiazole‑5‑carboxylic acid gave the corresponding ester[Fe_(2)(CO)_(6)(μ‑tedt)](2),where tedt=SCH_(2)CH(CH_(2)OOC(5‑C_(3)HNSCH_(3)))S.Further reactions of complex 2 with tri(ptolyl)phosphine(tp)or tris(4‑fluorophenyl)phosphine(fp)gave the phosphine‑substituted derivatives[Fe_(2)(CO)_(5)(tp)(μ‑tedt)](3)and[Fe_(2)(CO)_(5)(fp)(μ‑tedt)](4).The structures of the newly prepared complexes were elucidated by elemental analysis,NMR,IR,and X‑ray photoelectron spectroscopy.Moreover,single‑crystal X‑ray diffraction analysis confirmed their molecular structures,showing that they contain a di‑iron core ligated by a bridged dithiolate bearing a thiazole moiety and terminal carbonyls.The electrochemical and electrocatalytic proton reduction were probed by cyclic voltammetry,revealing that three complexes can catalyze the reduction of protons to H_(2) under the electrochemical conditions.For comparison,complex 4 possessed the best efficiency with a turnover frequency of 23.5 s^(-1)at 10 mmol·L^(-1)HOAc concentration.In addition,the fungicidal activity of these complexes was also investigated in this study.CCDC:2477511,2;2477512,3;2477513,4.
文摘Chitosan(CTS)was grafted onto the surface of amino‑functionalized silver chloride silicon dioxide(AgCl@SiO_(2)‑NH_(2))cores to obtain AgCl@SiO_(2)/CTS hybrid nanoparticles.The as‑obtained AgCl@SiO_(2)/CTS nanoparticles were chlorinated by NaClO solution to get AgCl@SiO_(2)/CTS‑based chloramine nano‑hybrid materials,denoted as AgCl@SiO_(2)/CTS‑Cl.A transmission electron microscope was used to observe the morphology of the as‑prepared samples AgCl@SiO_(2)/CTS and AgCl@SiO_(2)/CTS‑Cl.At the same time,an X‑ray diffractometer and an infrared spectroscope were utilized to characterize their crystal and chemical structures.Besides,ζpotentials were measured to elucidate the surface modification of AgCl nanoparticles by—NH_(2),the antibacterial mechanism of AgCl@SiO_(2)/CTS‑Cl was investigated by scanning electron microscopy,and Escherichia coli(E.coli)and Staphylococcus aureus(S.aureus)were used as the to‑be‑tested strains to evaluate the antimicrobial activity of samples AgCl@SiO_(2)/CTS and AgCl@SiO_(2)/CTS‑Cl.Findings demonstrate that sample AgCl@SiO_(2)/CTS exhibits a chain‑like structure ascribed to the interaction between—NH_(2),and each AgCl@SiO_(2)/CTS hybrid nanoparticle contains several AgCl cores.In the meantime,sample AgCl@SiO_(2)/CTS‑Cl exhibits excellent antibacterial activity against E.coli and S.aureus,which is attributed to the synergistic antibacterial effect of Ag^(+)and Cl^(-).Sample AgCl@SiO_(2)/CTS‑Cl with a dosage of 640.00μg·mL^(-1) could completely kill the two kinds of tested bacteria in 12 h of incubation;it retains a high antibacterial efficiency even after 10 cycles of antibacterial tests.
文摘BACKGROUND Postoperative delirium(POD)is a common and serious complication in surgical patients,particularly older adults.Alterations in cholinergic function have been implicated in its pathophysiology.AIM To evaluate the association between preoperative serum cholinesterase(ChE)activity—specifically butyrylcholinesterase(BuChE)and acetylcholinesterase(AChE)—and the risk of POD in adult surgical patients in a meta-analysis.METHODS A systematic search was conducted in PubMed,EMBASE,and Web of Science up to March 28,2025 for studies reporting preoperative serum BuChE or AChE activity in relation to subsequent POD incidence.Standardized mean differences(SMDs)and odds ratios(ORs)with 95%confidence intervals(CIs)were pooled using random-effects models.Subgroup and sensitivity analyses were performed based on follow-up duration and analytic models.RESULTS Thirteen studies(n=2730 patients)were included.Patients who developed POD had significantly lower preoperative BuChE activity than those who did not(SMD=-0.28;95%CI:-0.39 to-0.16;I^(2)=18%).Higher BuChE activity was associated with a reduced risk of POD(OR per 100 unit increment=0.97;95%CI:0.95-0.99;I2=0%).In contrast,pooled AChE activity did not differ significantly between POD and non-POD groups(SMD=-0.25;95%CI:-0.53 to 0.03;P=0.08;I^(2)=80%),and the ORs per 1 unit increment in AChE activity were not statistically significant(OR=0.98;95%CI:0.95-1.01).CONCLUSION Lower preoperative serum BuChE activity is associated with an increased risk of POD in adults undergoing surgery.BuChE activity may serve as a potential preoperative biomarker for POD risk stratification.
基金Supported by Zhaoqing University-Zhanjiang Institute for Food and Drug Control Joint Laboratory(52).
文摘[Objectives]This study was conducted to investigate the extraction process,content determination,and antioxidant properties of flavonoids from Hylocereus undatus(Haw.)Britton&Rose.[Methods]Using H.undatus as the raw material,the effects of ethanol concentration,ultrasonic temperature,time,and solid-to-liquid ratio on the total flavonoid yield were investigated through single-factor and orthogonal experiments.[Results]All factors had a significant effect on the yield.The optimized conditions were determined as follows:ethanol concentration 75%,ultrasonic temperature 60℃,ultrasonic time 30 min,and solid-to-liquid ratio 1:50(g/ml).Under these conditions,the total flavonoid yield reached 3.08%.Evaluation of antioxidant activity revealed that the extract exhibited superior scavenging rates against both DPPH and hydroxyl radicals compared with the standard reference compound BHT.[Conclusions]This study holds significant importance for elucidating the pharmacological mechanisms of flavonoids in H.undatus and for expanding their application in medicine and health products.
基金supported by the National Institutes of Health for the Kansas Center for Metabolism and Obesity Research(award No.P20GM144269)support from the Center for Advancing Translational Sciences of the National Institutes of Health(award No.KL2TR002367)supported by the National Center for Advancing Translational Sciences of the National Institutes of Health(award No.TL1TR002368)。
文摘Overweight and obesity are significant public health concerns worldwide due to their association with many chronic health conditions.This has resulted in the development of various interventions focused on weight loss to reduce the associated health burden.Physical activity is an important lifestyle behavior associated with enhanced health.Evidence supports that many of the benefits of physical activity are realized independent of initial weight status or whether weight loss is achieved,with some benefits additive to what is achieved with weight loss alone.These benefits include enhanced cardiometabolic,brain,cognitive and psychological health,and others.Moreover,in adults with overweight or obesity,physical activity has independent effects on cardiorespiratory fitness,muscular strength,physical function,and mobility.There are also benefits to body composition,with physical activity improving the quality of key tissues,such as skeletal muscle,which may not occur with diet-induced weight loss.Therefore,physical activity is an important public health target for adults with overweight or obesity to provide a wide range of health benefits that extend beyond those of weight loss alone.However,physical activity recommendations and programming efforts should consider the unique characteristics of adults with overweight or obesity to be most effective,and should support a focus on mobility,physical function,and other health outcomes.
基金supported by the National Natural Science Foundation of China(62325112)the National Key Research and Development Program of China(2023YFC2411700,2023YFC2411705)+2 种基金the National Natural Science Foundation of China(U22A2023)the National High-Level Hospital Clinical Research Funding(2022-PUMCH-C-009,2022-PUMCH-B-064,2022-PUMCH-D-002)the National Basic Research Program of China(973 Program,2014CB541801).
文摘Conventional ultrasound(US)evaluation of enthesitis in psoriatic arthritis(PsA)is limited by its inability to quantify metabolic alterations such as hypoxia,a key driver of disease activity.We introduce an oxygenation-integrated multimodal photoacoustic/ultrasound(PA/US)imaging framework designed to quantify entheseal oxygen saturation(SO_(2))for assessing entheseal disease activity in PsA.In this cross-sectional study,25 PsA patients underwent bilateral PA/US imaging of 12 entheses,where ultrasound lesions were scored using the Outcome Measures in Rheumatology scoring system,and PA-derived SO_(2) levels,quantified via dual-wavelength PA imaging,were classified into hyperoxia or hypoxia groups using k-means clustering.This approach provides metabolic insights complementary to conventional ultrasonic assessment.A composite score integrating hypoxia with US parameters was validated against clinical disease activity indices(Disease Activity Score 28-C-reactive protein,DAS28-CRP;Disease Activity Index for Psoriatic Arthritis,DAPSA).Among 300 entheses,103(34.3%)exhibited PA positivity,with 40(38.8%)classified as hypoxia.Hypoxia scores independently predicted DAS28-CRP(β=0.618,p=0.001)and DAPSA(β=0.612,p<0:001).The hypoxia-optimized PAUS score demonstrated superior correlation with disease activity indices compared to conventional US(DAS28-CRP:r=0.615,p=0.001 versus r=0.474,p=0.017;DAPSA:r=0.743,p<0:001 versus r=0.567,p=0.003),alongside superior diagnostic accuracy for minimal disease activity(area under the curve,AUC 0.776 versus 0.614,p=0.008)and low disease activity(AUC 0.853 versus 0.772,p=0.009).This multimodal scoring system enhances the stratification of PsA disease activity by providing unique metabolic insights,offering a potential tool for therapeutic monitoring and guiding treat-to-target strategies.
基金Supported by Provincial Key Research Project of Henan Province,No.232102310081.
文摘BACKGROUND Major depressive disorder(MDD)and obesity(OB)are bidirectionally comorbid conditions with common neurobiological underpinnings.However,the neurocognitive mechanisms of their comorbidity remain poorly understood.AIM To examine regional abnormalities in spontaneous brain activity among patients with MDD-OB comorbidity.METHODS This study adopted a regional homogeneity(ReHo)analysis of resting-state functional magnetic resonance imaging.The study included 149 hospital patients divided into four groups:Patients experiencing their first episode of drug-naive MDD with OB,patients with MDD without OB,and age-and sex-matched healthy individuals with and without OB.Whole-brain ReHo analysis was conducted using SPM12 software and RESTplus toolkits,with group comparisons via ANOVA and post-hoc tests.Correlations between ReHo values and behavioral measures were examined.RESULTS ANOVA revealed significant whole-brain ReHo differences among the four groups in four key regions:The left middle temporal gyrus(MTG.L),right cuneus,left precuneus,and left thalamus.Post-hoc analyses confirmed pairwise differences between all groups across these regions(P<0.05).OB was associated with ReHo alterations in the MTG.L,right cuneus,and left thalamus,whereas abnormalities in the precuneus suggested synergistic pathological mechanisms between MDD and OB.Statistically significant correlations were found between the drive and fun-seeking dimensions of the behavioral activation system,as well as behavioral inhibition and the corresponding ReHo values.CONCLUSION Our findings provide novel evidence for the neuroadaptive mechanisms underlying the MDD-OB comorbidity.Further validation could lead to personalized interventions targeting MTG.L hyperactivity and targeting healthy food cues.
基金supported by Research Projects of Liaoning Provincial Department of Education(LJ212410163008)the National Training Program of Innovation and Entrepreneurship for Undergraduates of Shenyang Pharmaceutical University(202410163042)。
文摘The photothermal effect enhances the antibacterial activity by generating localized heat that disrupts microbial cells,leading to increased bacterial inactivation.The objective of this study was to develop and evaluate the antibacterial and photothermal applications of polydopamine-berberine nanoparticles(PDA-BBR-NPs),incorporating them into a sodium alginate matrix for food preservation.The self-assembled PDA-BBR-NPs were synthesized using a solution oxidation method and characterized by scanning electron microscopy,Fourier transform infrared spectroscopy,and nanoparticle size analysis.The results demonstrated that PDA-BBR-NPs were uniform spheres with an average diameter of 177.1 nm.Drug loading and encapsulation efficiency tests showed that the optimal formulation had a drug loading capacity of 48.4%and an encapsulation efficiency of 84.70%.Antibacterial activity against Escherichia coli and Staphylococcus aureus was evaluated using the dilution coating plate method,showing an inhibition rate of over 98%.Photothermal testing under 808 nm near-infrared irradiation revealed a maximum photothermal conversion efficiency of 32.65%.To assess the practical application of the nanoparticles,they were incorporated into a sodium alginate-based coating for the preservation of Kyoho grapes.Key parameters such as weight loss rate,pH,titratable acidity,and soluble solids content were monitored over a 12-day storage period at room temperature.The PDA-BBR-near-infrared spectroscopy-treated group exhibited significantly reduced weight loss rate(6.08%lower than the control group)and a slower decrease in pH.The coating effectively inhibited microbial activity and extended the shelf life of the grapes by 3-6 days compared to the control group.These findings demonstrated the potential of PDA-BBR-NPs to enhance the preservation of fresh produce through their combined antibacterial and photothermal effects,offering a promising method for extending fruit shelf life.
文摘Background:Early detection of harmful brain activity in critically ill patients using electroencephalography(EEG)is vital for timely and effective clinical intervention.Automating EEG analysis with deep learning techniques holds significant promise for enhancing diagnostic efficiency and accuracy.Methods:We implemented EfficientNetB2,which leverages convolutional neural networks with a novel Temporal Squeeze-and-Excitation module to capture temporal EEG features,and WaveNet,a sequential model designed to effectively model temporal dependencies in EEG data using dilated causal convolutions and temporal self-attention.Both models were trained and evaluated using a publicly available EEG dataset,with performance assessed via 4-fold cross-validation and a step-wise learning rate reduction strategy.Results:Our results demonstrate a significant reduction in training loss from 0.6459 to 0.3055 and validation loss from 0.9602 to 0.5719 over six epochs.Consistent improvements were observed across cross-validation folds,highlighting the robustness of the models.Additionally,ensemble learning of the two architectures further enhanced classification performance.Conclusion:This comparative analysis sheds light on the strengths and limitations of EfficientNetB2 and WaveNet for automated harmful brain activity detection in EEG signals.The findings contribute to the advancement of reliable and efficient deep learning models,paving the way for their clinical application in managing critically ill patients.
基金National Key Research and Development Program of China(No.2022YFD1802104).
文摘Aberrant activation of Receptor Tyrosine Kinases(RTKs)is a well-established trigger of tumorigenesis,and the over-use of RTK inhibitors often leads to drug resistance and tumor recurrence.While current Drug-Target Interaction(DTI)prediction methods(including those based on heterogeneous information networks)have shown promise,they remain limited in their ability to fully capture the nature of DTIs and often lack interpretability.To overcome these limitations,this study introduces a novel hybrid optimization model termed MDBO-RF,which integrates a Modified Dung Beetle Optimizer(MDBO)with Random Forest(RF).The key innovation lies in the enhancement of the DBO algorithm through a quaternion-based learning mechanism and the Cauchy mutation strategy,specifically designed to overcome the slow convergence and susceptibility to local optima that plague traditional metaheuristic algorithms used for hyperparameter tuning.The model leverages commonly used molecular descriptors to enhance the prediction of Tyrosine Kinase(TK)inhibitory activity and enable efficient compound screening.Our results demonstrate that MDBO-RF achieves a 3.41%increase in prediction accuracy compared to the standard RF model and outperforms several other contemporary machine learning approaches.The model effectively streamlines the RTK inhibitor screening process by improving prediction accuracy in multi-target competitive binding scenarios and reducing false-positive screening due to off-target effects.This work underscores the value of hybrid optimization strategies in bioinformatics and provides a robust,interpretable tool for accelerating drug discovery.
基金supported by Princess Nourah bint Abdulrahman University Researchers Supporting Project number(PNURSP2026R765),Princess Nourah bint Abdulrahman University,Riyadh,Saudi Arabia.
文摘Human Activity Recognition(HAR)is a novel area for computer vision.It has a great impact on healthcare,smart environments,and surveillance while is able to automatically detect human behavior.It plays a vital role in many applications,such as smart home,healthcare,human computer interaction,sports analysis,and especially,intelligent surveillance.In this paper,we propose a robust and efficient HAR system by leveraging deep learning paradigms,including pre-trained models,CNN architectures,and their average-weighted fusion.However,due to the diversity of human actions and various environmental influences,as well as a lack of data and resources,achieving high recognition accuracy remain elusive.In this work,a weighted average ensemble technique is employed to fuse three deep learning models:EfficientNet,ResNet50,and a custom CNN.The results of this study indicate that using a weighted average ensemble strategy for developing more effective HAR models may be a promising idea for detection and classification of human activities.Experiments by using the benchmark dataset proved that the proposed weighted ensemble approach outperformed existing approaches in terms of accuracy and other key performance measures.The combined average-weighted ensemble of pre-trained and CNN models obtained an accuracy of 98%,compared to 97%,96%,and 95%for the customized CNN,EfficientNet,and ResNet50 models,respectively.
文摘The Almus Fault Zone(AFZ)is one of the major splay faults of the North Anatolian Fault Zone(NAFZ)and is important for understanding its tectonic features and assessing regional seismic hazards.This research presents the integration of morphometric indices to quantitatively assess the spatial variation of tectonic activity along the AFZ.The AFZ is an active fault with both strike-slip and normal fault components and consists of two main branches,Mercimekdağı-Çamdere Fault(MÇF)and Tokat Fault(TF)segments.This study aims to assess the relative tectonic activity of the AFZ using various morphometric indices,based on a 10 m resolution DEM,with the aid of ArcGIS and MATLAB software.For this purpose,morphometric indices such as hypsometric integral(HI:0.35-0.65),mountain front sinuosity(Smf:1.3-1.44),valley floor width-height ratio(Vf:0.15-2.28),asymmetry factor(AF:23-77),drainage basin shape(Bs:1.13-6.10)and normalized steepness index(ksn:1-498)were applied to 53 drainage basins.When the Smf and mean Vf indices results were evaluated,it was calculated that the uplift ratio of the region was more than 0.5 mm/yr.The spatial distribution of the relative tectonic activity(Iat)of the area was revealed by combining the obtained morphometric indices analysis results.According to the Iat result,it was concluded that the MercimekdağıÇamdere Fault and Tokat Fault segments have high tectonic activity,but the Mercimekdağı-Çamdere Fault segment has higher tectonic activity.The results obtained were also confirmed by field observations.This research provides valuable information for the evaluation of tectonic activity in drainage systems controlled by splay faults.
