phytohemagglutinin(PHA)-induced swelling is widely used to investigate cell-mediated and innate immunity across different vertebrate taxa.However,its physiological mechanism is still an open question due to the comple...phytohemagglutinin(PHA)-induced swelling is widely used to investigate cell-mediated and innate immunity across different vertebrate taxa.However,its physiological mechanism is still an open question due to the complexity of the involved immune components.In the present study,we measured the synchronous variations of PHA response,the proportion of different subtypes of leukocytes,as well as serum bactericidal capacity in circulation blood at 6,12 and 24 h after PHA versus PBS injection in striped hamster,Cricetulus barabensis.First,the results showed that PHA responses reached a peak at 6 h postinjection,then sharply declined at 12 h and 24 h postinjection.Serum bactericidal capacity was higher at 6 h and 12 h than at 24 h.The proportion of different subtypes of leukocytes,as well as the ratio of neutrophils to lymphocytes did not display significant changes across different time points.Second,PHA response was positively correlated with the proportion of neutrophils and serum bactericidal capacity.The proportion of monocytes was negatively correlated with that of eosinophils and neutrophils.The proportion of basophils was negatively correlated with that of lymphocytes.Our results indicate that earlier enhanced PHA response is important for the striped hamster to cope with changing environmental conditions due to its small body mass,and the increased components of innate immunity in circulation blood may contribute to the enhancement of PHA swelling response.展开更多
Although occupying pillar position in clinical cancer treatments,surgery itself and surgical trauma would elicit series of local/systemic inflammation-related responses that resulted in high rate of tumor recurrence.H...Although occupying pillar position in clinical cancer treatments,surgery itself and surgical trauma would elicit series of local/systemic inflammation-related responses that resulted in high rate of tumor recurrence.Herein,chitosan with conjugated gallic acid(CSG)molecules were coordinated with Fe3+to form CSG/Fe3+hydrogel for filling the tumo r-resected cavity with considerable wet-adhesion ability and anti-inflammatory performance.With the assistance of doxorubicin hydrochloride(DOX·HCl),CSG/Fe3+/DOX hydrogel exhibited syne rgistic photothermal-chemo tumo r-inhibited performance under near-infrared(NIR)light irradiation for eradicating residual and/or surgical trauma-recruited cancer cells.Thus,our study attempts to show a paradigm that realizes quick surgical trauma healing,inflammation inhibition and prevention of postsurgical tumor recurrence.展开更多
基金This study was supported by a Key Project of Natural Science of the Anhui Province Department of Education,China(KJ2011A114)supported by the State Key Laboratory of Integrated Management of Pest Insects and Rodents,Institute of Zoology,The Chinese Academy of Sciences(ChineseIPM1001)to ZZQ.
文摘phytohemagglutinin(PHA)-induced swelling is widely used to investigate cell-mediated and innate immunity across different vertebrate taxa.However,its physiological mechanism is still an open question due to the complexity of the involved immune components.In the present study,we measured the synchronous variations of PHA response,the proportion of different subtypes of leukocytes,as well as serum bactericidal capacity in circulation blood at 6,12 and 24 h after PHA versus PBS injection in striped hamster,Cricetulus barabensis.First,the results showed that PHA responses reached a peak at 6 h postinjection,then sharply declined at 12 h and 24 h postinjection.Serum bactericidal capacity was higher at 6 h and 12 h than at 24 h.The proportion of different subtypes of leukocytes,as well as the ratio of neutrophils to lymphocytes did not display significant changes across different time points.Second,PHA response was positively correlated with the proportion of neutrophils and serum bactericidal capacity.The proportion of monocytes was negatively correlated with that of eosinophils and neutrophils.The proportion of basophils was negatively correlated with that of lymphocytes.Our results indicate that earlier enhanced PHA response is important for the striped hamster to cope with changing environmental conditions due to its small body mass,and the increased components of innate immunity in circulation blood may contribute to the enhancement of PHA swelling response.
基金the National Natural Science Foundation of China(Nos.31800834,51572067)the University Synergy Innovation Program of Anhui Province(No.GXXT-2019-045)the Fundamental Research Funds for the Central Universities(Nos.JZ2018HGPA0273,JZ2018HGPA0269,JZ2018HGTB0247)。
文摘Although occupying pillar position in clinical cancer treatments,surgery itself and surgical trauma would elicit series of local/systemic inflammation-related responses that resulted in high rate of tumor recurrence.Herein,chitosan with conjugated gallic acid(CSG)molecules were coordinated with Fe3+to form CSG/Fe3+hydrogel for filling the tumo r-resected cavity with considerable wet-adhesion ability and anti-inflammatory performance.With the assistance of doxorubicin hydrochloride(DOX·HCl),CSG/Fe3+/DOX hydrogel exhibited syne rgistic photothermal-chemo tumo r-inhibited performance under near-infrared(NIR)light irradiation for eradicating residual and/or surgical trauma-recruited cancer cells.Thus,our study attempts to show a paradigm that realizes quick surgical trauma healing,inflammation inhibition and prevention of postsurgical tumor recurrence.
