Bacterial infection is rising as a threatening health issue.Because of the present delay in early diagnosis of bacterial diseases as well as the abuse of antibiotics,it has become a vital issue in the development of i...Bacterial infection is rising as a threatening health issue.Because of the present delay in early diagnosis of bacterial diseases as well as the abuse of antibiotics,it has become a vital issue in the development of in-time detection and therapy of bacterial infections.Herein,we designed a multifunctional nanotheranostics platform based on the unique micro-environment of bacterial infections to achieve specific bioimaging and simultaneous inactivation of the target bacteria.We showed that in bacterial infections,the metal precursors(i.e.,HAuCl_(4),FeCl_(2),and herring sperm DNA)could be readily bio-self-assembled to multifunctional nanoclusters(NCs)that exhibit luminescence,in which AuCl_(4)-was biosynthesized via reductive biomolecules such as NADPH to the fluorescent AuNCs.The DNA may assist as an encapsulation and delivery vector,and Fe^(2+)served as a fluorescence intensifier and reduced reactive oxygen species(ROS)to produce the iron oxides.While the bacteria were being visualized,the microenvironment-responsive NCs were enabled to sterilize bacteria efficiently due to electrostatic effect,cell membrane destruction,inhibition of biofilm formation,and ROS accumulation.Besides,the bio-responsive self-assembled NCs complexes contributed to accelerating bacteria-infected wound healing and showed negligible side effects in long-term toxicity tests in vivo.Also,intracellular molecules involved in microenvironmental response were investigated.The work may become an effective strategy for the detection and real-time sterilization of intractable bacterial infections.展开更多
基金This work was supported by the National Key Research and Development Program of China(No.2017YFA0205300)the National Natural Science Foundation of China(Nos.82027806,82061148012,and 91753106)+1 种基金Primary Research&Development Plan of Jiangsu Province(No.BE2019716)the ISF-NSFC Joint Research Program(No.3258/20)to Y.W.
文摘Bacterial infection is rising as a threatening health issue.Because of the present delay in early diagnosis of bacterial diseases as well as the abuse of antibiotics,it has become a vital issue in the development of in-time detection and therapy of bacterial infections.Herein,we designed a multifunctional nanotheranostics platform based on the unique micro-environment of bacterial infections to achieve specific bioimaging and simultaneous inactivation of the target bacteria.We showed that in bacterial infections,the metal precursors(i.e.,HAuCl_(4),FeCl_(2),and herring sperm DNA)could be readily bio-self-assembled to multifunctional nanoclusters(NCs)that exhibit luminescence,in which AuCl_(4)-was biosynthesized via reductive biomolecules such as NADPH to the fluorescent AuNCs.The DNA may assist as an encapsulation and delivery vector,and Fe^(2+)served as a fluorescence intensifier and reduced reactive oxygen species(ROS)to produce the iron oxides.While the bacteria were being visualized,the microenvironment-responsive NCs were enabled to sterilize bacteria efficiently due to electrostatic effect,cell membrane destruction,inhibition of biofilm formation,and ROS accumulation.Besides,the bio-responsive self-assembled NCs complexes contributed to accelerating bacteria-infected wound healing and showed negligible side effects in long-term toxicity tests in vivo.Also,intracellular molecules involved in microenvironmental response were investigated.The work may become an effective strategy for the detection and real-time sterilization of intractable bacterial infections.
