Objective:To evaluate the clinical efficacy of cefixime(Cef)combined with montmorillonite powder(MP)in treating pediatric bacterial enteritis.Methods:Seventy-two cases of bacterial enteritis in children admitted betwe...Objective:To evaluate the clinical efficacy of cefixime(Cef)combined with montmorillonite powder(MP)in treating pediatric bacterial enteritis.Methods:Seventy-two cases of bacterial enteritis in children admitted between October 2021 and October 2023 were selected and randomly divided into two groups.The combination group received Cef treatment alongside MP,while the control group received only Cef treatment.The groups were compared based on overall efficacy,symptom relief time,and other indicators.Results:The combination group showed a higher overall efficacy,shorter symptom relief time,lower levels of inflammatory markers post-treatment,and a higher proportion of normal and grade I dysbiosis in the intestinal flora,with significant differences compared to the control group(P<0.05).Conclusion:Cef combined with MP is significantly effective in treating pediatric bacterial enteritis,promoting symptom relief,reducing inflammation,and correcting intestinal flora imbalance.展开更多
BACKGROUND Bacterial contamination during colonoscopy is a significant concern,yet few studies have evaluated bacterial aerosols.This study aimed to determine whether covering the biopsy hole check valve with enzymoly...BACKGROUND Bacterial contamination during colonoscopy is a significant concern,yet few studies have evaluated bacterial aerosols.This study aimed to determine whether covering the biopsy hole check valve with enzymolysis gauze(refers to sterile gauze soaked in a multi-enzyme cleaning solution)reduces bacterial air pollution in endoscopy rooms.AIM To evaluate the efficacy of an enzymolysis gauze cover in reducing bacterial aerosols from the biopsy valve.METHODS This prospective,single-blind trial included 80 patients undergoing elective diagnostic colonoscopy.During the procedure,the biopsy hole check valve was either covered or left uncovered with enzymolysis gauze.Air samples(100 L)were collected at a distance of 30 cm from the biopsy hole check valve and approximately 140 cm above the floor using a percussive air sampling instrument.Gram-positive bacteria were cultured on standard 90 mm colimycin nalidixic agar blood plates.The primary outcome measures were bacterial load and species identification.RESULTS Covering the biopsy hole check valve with enzymolysis gauze reduced bacterial load near the check valve from 50 colony-forming unit(CFU)/m^(3)[interquartile range(IQR):30-80]to 20 CFU/m^(3)(IQR:10-20).At the end of the procedure each day,covering the valve also decreased bacterial load in the endoscopy room from 35 CFU/m^(3)(IQR:33-85)to 10 CFU/m^(3)(IQR:5-10).The predominant bacteria identified were Gram-positive cocci.CONCLUSION Applying enzymolysis gauze to cover the biopsy hole check valve significantly reduces bacterial aerosol contamination in endoscopy rooms during colonoscopy.展开更多
Bacteriophages(phages)exhibit remarkable specificity and efficacy in eliminating pathogenic bacteria,marking a paradigm shift in the battle against drug-resistant pathogens and presenting themselves as a promising alt...Bacteriophages(phages)exhibit remarkable specificity and efficacy in eliminating pathogenic bacteria,marking a paradigm shift in the battle against drug-resistant pathogens and presenting themselves as a promising alternative to traditional antibiotics1,2.A growing body of clinical research supports this trend,with phage-based therapies being assessed across a range of infectious conditions,including gastrointestinal infections,pulmonary bacterial disorders,and genitourinary system infections3-5.展开更多
Background:Gut ischemia and hypoxia post severe burn leads to breakdown of intestinal epithelial barrier and enteric bacterial translocation(EBT),resulting in serious complications,such as systemic inflammatory respon...Background:Gut ischemia and hypoxia post severe burn leads to breakdown of intestinal epithelial barrier and enteric bacterial translocation(EBT),resulting in serious complications,such as systemic inflammatory response syndrome,sepsis and multiple organ failure.Cystic fibrosis transmembrane conductance regulator(CFTR)is known to be downregulated by hypoxia and modulate junctional complexes,which are crucial structures maintaining the intestinal barrier.This study aimed to investigate whether CFTR plays a role in both regulating the intestinal barrier and mediating EBT post severe burn,as well as the signaling pathways involved in these processes.Methods:An in vitro Caco-2 cell model subjected to hypoxic injury and an in vivo mouse model with a 30%total body surface area full-thickness dermal burn were established.DF 508 mice(mice with F508del CFTR gene mutation)were used as an in vivo model to further demonstrate the role of CFTR in maintaining normal intestinal barrier function.QRT-PCR,western blot,ELISA,TER assay and immunofluorescence staining were used to detect the expression and localization of CFTR and tight junction proteins,as well as the function of tight junctions.Results:Our data indicated that,in Caco-2 cells,the hypoxia condition significantly reduced CFTR expression;activated extracellular signal-regulated kinase and nuclear factor-κB signaling;elevated secretion of inflammatory factors(tumor necrosis factor-α,interleukin-1βand interleukin-8);downregulated zonula occludens-1,occludin and E-cadherin expression;decreased transepithelial electrical resistance values;and led to a cellular mislocation of ZO-1.More importantly,knockdown of CFTR caused similar alterations.The upregulation of inflammatory factors and downregulation of tight junction proteins(ZO-1 and occludin)induced by knockdown of CFTR could be reversed by specific extracellular signal-regulated kinase or nuclear factor-κB inhibition.In support of the in vitro data,exuberant secretion of pro-inflammatory mediators and EBT was observed in the intestine of severely burnt mice in vivo.EBT occurred in DF508 mice(mice with the F508del CFTR gene mutation),accompanied by augmented tumor necrosis factor-α,interleukin-1βand interleukin-8 levels in the ileum compared to wildtype mice.In addition,vitamin D3 was shown to protect the intestinal epithelial barrier from hypoxic injury.Conclusions:Collectively,the present study illustrated that CFTR and downstream signaling were critical in modulating the intestinal epithelial junction and EBT post severe burn.展开更多
文摘Objective:To evaluate the clinical efficacy of cefixime(Cef)combined with montmorillonite powder(MP)in treating pediatric bacterial enteritis.Methods:Seventy-two cases of bacterial enteritis in children admitted between October 2021 and October 2023 were selected and randomly divided into two groups.The combination group received Cef treatment alongside MP,while the control group received only Cef treatment.The groups were compared based on overall efficacy,symptom relief time,and other indicators.Results:The combination group showed a higher overall efficacy,shorter symptom relief time,lower levels of inflammatory markers post-treatment,and a higher proportion of normal and grade I dysbiosis in the intestinal flora,with significant differences compared to the control group(P<0.05).Conclusion:Cef combined with MP is significantly effective in treating pediatric bacterial enteritis,promoting symptom relief,reducing inflammation,and correcting intestinal flora imbalance.
基金Supported by the Construction Fund of Key Medical Disciplines of Hangzhou,No.2025HZGF05the Key R and D Program of Zhejiang Province,No.2023C03054 and No.2024C03048+1 种基金the Key Discipline of Integrated Chinese and Western Medicine(Digestive Diseases)in Zhejiang Province,No.2024-XK-61the Zhejiang Province Medical and Health Science and Technology Plan Project,No.2021KY848.
文摘BACKGROUND Bacterial contamination during colonoscopy is a significant concern,yet few studies have evaluated bacterial aerosols.This study aimed to determine whether covering the biopsy hole check valve with enzymolysis gauze(refers to sterile gauze soaked in a multi-enzyme cleaning solution)reduces bacterial air pollution in endoscopy rooms.AIM To evaluate the efficacy of an enzymolysis gauze cover in reducing bacterial aerosols from the biopsy valve.METHODS This prospective,single-blind trial included 80 patients undergoing elective diagnostic colonoscopy.During the procedure,the biopsy hole check valve was either covered or left uncovered with enzymolysis gauze.Air samples(100 L)were collected at a distance of 30 cm from the biopsy hole check valve and approximately 140 cm above the floor using a percussive air sampling instrument.Gram-positive bacteria were cultured on standard 90 mm colimycin nalidixic agar blood plates.The primary outcome measures were bacterial load and species identification.RESULTS Covering the biopsy hole check valve with enzymolysis gauze reduced bacterial load near the check valve from 50 colony-forming unit(CFU)/m^(3)[interquartile range(IQR):30-80]to 20 CFU/m^(3)(IQR:10-20).At the end of the procedure each day,covering the valve also decreased bacterial load in the endoscopy room from 35 CFU/m^(3)(IQR:33-85)to 10 CFU/m^(3)(IQR:5-10).The predominant bacteria identified were Gram-positive cocci.CONCLUSION Applying enzymolysis gauze to cover the biopsy hole check valve significantly reduces bacterial aerosol contamination in endoscopy rooms during colonoscopy.
基金supported by the National Natural Science Foundation of China(82472132)the Distinguished Young Scholars of Chongqing(2022NSCQ-JQX5279,China).
文摘Bacteriophages(phages)exhibit remarkable specificity and efficacy in eliminating pathogenic bacteria,marking a paradigm shift in the battle against drug-resistant pathogens and presenting themselves as a promising alternative to traditional antibiotics1,2.A growing body of clinical research supports this trend,with phage-based therapies being assessed across a range of infectious conditions,including gastrointestinal infections,pulmonary bacterial disorders,and genitourinary system infections3-5.
文摘Background:Gut ischemia and hypoxia post severe burn leads to breakdown of intestinal epithelial barrier and enteric bacterial translocation(EBT),resulting in serious complications,such as systemic inflammatory response syndrome,sepsis and multiple organ failure.Cystic fibrosis transmembrane conductance regulator(CFTR)is known to be downregulated by hypoxia and modulate junctional complexes,which are crucial structures maintaining the intestinal barrier.This study aimed to investigate whether CFTR plays a role in both regulating the intestinal barrier and mediating EBT post severe burn,as well as the signaling pathways involved in these processes.Methods:An in vitro Caco-2 cell model subjected to hypoxic injury and an in vivo mouse model with a 30%total body surface area full-thickness dermal burn were established.DF 508 mice(mice with F508del CFTR gene mutation)were used as an in vivo model to further demonstrate the role of CFTR in maintaining normal intestinal barrier function.QRT-PCR,western blot,ELISA,TER assay and immunofluorescence staining were used to detect the expression and localization of CFTR and tight junction proteins,as well as the function of tight junctions.Results:Our data indicated that,in Caco-2 cells,the hypoxia condition significantly reduced CFTR expression;activated extracellular signal-regulated kinase and nuclear factor-κB signaling;elevated secretion of inflammatory factors(tumor necrosis factor-α,interleukin-1βand interleukin-8);downregulated zonula occludens-1,occludin and E-cadherin expression;decreased transepithelial electrical resistance values;and led to a cellular mislocation of ZO-1.More importantly,knockdown of CFTR caused similar alterations.The upregulation of inflammatory factors and downregulation of tight junction proteins(ZO-1 and occludin)induced by knockdown of CFTR could be reversed by specific extracellular signal-regulated kinase or nuclear factor-κB inhibition.In support of the in vitro data,exuberant secretion of pro-inflammatory mediators and EBT was observed in the intestine of severely burnt mice in vivo.EBT occurred in DF508 mice(mice with the F508del CFTR gene mutation),accompanied by augmented tumor necrosis factor-α,interleukin-1βand interleukin-8 levels in the ileum compared to wildtype mice.In addition,vitamin D3 was shown to protect the intestinal epithelial barrier from hypoxic injury.Conclusions:Collectively,the present study illustrated that CFTR and downstream signaling were critical in modulating the intestinal epithelial junction and EBT post severe burn.
