AIM: To understand the pathogen characteristics and its sensitivity against antimicrobial agents in fatal bacterial granuloma after eyelid trauma (FBGT) in vitro, and to provide laboratory evidence for diagnosis. METH...AIM: To understand the pathogen characteristics and its sensitivity against antimicrobial agents in fatal bacterial granuloma after eyelid trauma (FBGT) in vitro, and to provide laboratory evidence for diagnosis. METHODS: The FBGT pathogens were isolaated and cultured with reformed rabbit-brain anaerobic enriched broth (RRAB), and identified by ATB/API 20A system. The minimum inhibiting concentration (MIC) was determined by anaerobic broth dilution method. RESULTS: A total of 22 strains of pathogen were separated from 21 patients with FBGT and identified as Propionibacterium acnes (PA) by ATB/API 20A system. The MIC of ciprofloxacin for 22 PA strains was 0.0625-0.5mg/L, the MIC of penicillin, ampicillin, ampicillin/sulbactam, cefoperazone, lincomycin, and imipenem/cilastatin were 0.125-0.5mg/L, the MIC of ticarcillin/clavulanic acid was 0.250-1.000mg/L, and the MIC of metronidazole was 64-256mg/L. The pathogen of FBGT was strictly anaerobic PA, which growed slowly and better in nutritious RRAB broth. All PA were resistant to metronidazole, but susceptive to other routine antimicrobial agents, such as penicillin, ampicillin and lincomycin. CONCLUSION: FBGT should not be treated with metronidazole. Clinicians should choose combined use of drugs or operation to treat FBGT according to patients' individual condition and the results of drug sensitivity test.展开更多
【目的】探讨光合细菌菌剂在不同浓度下对番荔枝果园土壤微环境及果实品质的改善效果,为番荔枝的绿色施肥提供科学依据。【方法】以‘Gefner’番荔枝为试验材料,设置了4个不同施用量的光合细菌菌剂处理,即每160 m^(2)处理区喷施300(T_(...【目的】探讨光合细菌菌剂在不同浓度下对番荔枝果园土壤微环境及果实品质的改善效果,为番荔枝的绿色施肥提供科学依据。【方法】以‘Gefner’番荔枝为试验材料,设置了4个不同施用量的光合细菌菌剂处理,即每160 m^(2)处理区喷施300(T_(1))、600(T_(2))、900(T_(3))、1200 m L(T_(4))的菌剂,同时设立喷施清水为对照(CK)处理,研究不同浓度光合细菌菌剂对果园土壤理化性质、酶活性、微生物数量及果实品质的影响。【结果】与CK相比,光合细菌菌剂的施用显著改善了番荔枝果园的土壤微环境和果实品质。在T_(3)处理下,番荔枝果园土壤的理化性质得到了显著提高,其中有机质含量、碱解氮含量、有效磷含量、速效钾含量和阳离子交换量等指标分别提升了18.06%、35.68%、20.88%、52.20%、15.65%;同时,酶活性也得到了显著增强,脲酶活性、蛋白酶活性、过氧化氢酶活性和蔗糖酶活性等分别提高了21.88%、10.93%、19.61%、23.09%;此外,土壤微生物生态得到有效调节,细菌数量和放线菌数量显著增加了50.44%、28.46%,而真菌数量则显著减少了18.73%;有效地提高果实的品质和产量,果实中的总糖含量、总酸含量、可溶性固形物含量、维生素C含量等分别提高了10.10%、11.65%、6.54%、28.48%,同时果实的产量也显著提高了8.33%。【结论】光合细菌菌剂的施用对番荔枝果园的土壤理化性质和微生物生态具有显著的改善作用,能够提高土壤中细菌和放线菌的数量,降低土壤中的真菌数量,同时显著提高番荔枝的果实产量与品质。其中,T_(3)处理的光合细菌菌剂对番荔枝的综合改善效果最为明显。展开更多
文摘AIM: To understand the pathogen characteristics and its sensitivity against antimicrobial agents in fatal bacterial granuloma after eyelid trauma (FBGT) in vitro, and to provide laboratory evidence for diagnosis. METHODS: The FBGT pathogens were isolaated and cultured with reformed rabbit-brain anaerobic enriched broth (RRAB), and identified by ATB/API 20A system. The minimum inhibiting concentration (MIC) was determined by anaerobic broth dilution method. RESULTS: A total of 22 strains of pathogen were separated from 21 patients with FBGT and identified as Propionibacterium acnes (PA) by ATB/API 20A system. The MIC of ciprofloxacin for 22 PA strains was 0.0625-0.5mg/L, the MIC of penicillin, ampicillin, ampicillin/sulbactam, cefoperazone, lincomycin, and imipenem/cilastatin were 0.125-0.5mg/L, the MIC of ticarcillin/clavulanic acid was 0.250-1.000mg/L, and the MIC of metronidazole was 64-256mg/L. The pathogen of FBGT was strictly anaerobic PA, which growed slowly and better in nutritious RRAB broth. All PA were resistant to metronidazole, but susceptive to other routine antimicrobial agents, such as penicillin, ampicillin and lincomycin. CONCLUSION: FBGT should not be treated with metronidazole. Clinicians should choose combined use of drugs or operation to treat FBGT according to patients' individual condition and the results of drug sensitivity test.
文摘【目的】探讨光合细菌菌剂在不同浓度下对番荔枝果园土壤微环境及果实品质的改善效果,为番荔枝的绿色施肥提供科学依据。【方法】以‘Gefner’番荔枝为试验材料,设置了4个不同施用量的光合细菌菌剂处理,即每160 m^(2)处理区喷施300(T_(1))、600(T_(2))、900(T_(3))、1200 m L(T_(4))的菌剂,同时设立喷施清水为对照(CK)处理,研究不同浓度光合细菌菌剂对果园土壤理化性质、酶活性、微生物数量及果实品质的影响。【结果】与CK相比,光合细菌菌剂的施用显著改善了番荔枝果园的土壤微环境和果实品质。在T_(3)处理下,番荔枝果园土壤的理化性质得到了显著提高,其中有机质含量、碱解氮含量、有效磷含量、速效钾含量和阳离子交换量等指标分别提升了18.06%、35.68%、20.88%、52.20%、15.65%;同时,酶活性也得到了显著增强,脲酶活性、蛋白酶活性、过氧化氢酶活性和蔗糖酶活性等分别提高了21.88%、10.93%、19.61%、23.09%;此外,土壤微生物生态得到有效调节,细菌数量和放线菌数量显著增加了50.44%、28.46%,而真菌数量则显著减少了18.73%;有效地提高果实的品质和产量,果实中的总糖含量、总酸含量、可溶性固形物含量、维生素C含量等分别提高了10.10%、11.65%、6.54%、28.48%,同时果实的产量也显著提高了8.33%。【结论】光合细菌菌剂的施用对番荔枝果园的土壤理化性质和微生物生态具有显著的改善作用,能够提高土壤中细菌和放线菌的数量,降低土壤中的真菌数量,同时显著提高番荔枝的果实产量与品质。其中,T_(3)处理的光合细菌菌剂对番荔枝的综合改善效果最为明显。
