In this study,two wheat-derived cadmium(Cd)-immobilizing endophytic Pseudomonas paralactis M14 and Priestia megaterium R27 were evaluated for their effects on wheat tissue Cd uptake under hydroponic conditions.Then,th...In this study,two wheat-derived cadmium(Cd)-immobilizing endophytic Pseudomonas paralactis M14 and Priestia megaterium R27 were evaluated for their effects on wheat tissue Cd uptake under hydroponic conditions.Then,the impacts of the biochar(BC),M14+R27(MR),and BC+MR treatments on wheat Cd uptake and the mechanisms involved were investigated at the jointing,heading,and mature stages of wheat plants under field-plot conditions.A hydroponic experiment showed that the MR treatment significantly decreased the above-ground tissue Cd content compared with theM14 or R27 treatment.The BC+MRtreatment reduced the grain Cd content by 51.5%-67.7%and Cd translocation factor at the mature stage of wheat plants and increased the organic matter-bound Cd content by 31%-75%in the rhizosphere soils compared with the BC or MR treatment.Compared with the BC or MR treatment,the relative abundances of the biomarkers associated with Gemmatimonas,Altererythrobacter,Gammaproteobacteria,Xanthomonadaceae,Phenylobacterium,and Nocardioides in the BC+MR-treated rhizosphere microbiome decreased and negatively correlated with the organic matter-bound Cd contents.In the BC+MR-treated root interior microbiome,the relative abundance of the biomarker belonging to Exiguobacterium increased and negatively correlated with the Cd translocation factor,while the relative abundance of the biomarker belonging to Pseudonocardiaceae decreased and positively correlated with the Cd translocation factor.Our findings suggested that the BC+MR treatment reduced Cd availability and Cd transfer through affecting the abundances of these specific biomarkers in the rhizosphere soil and root interior microbiomes,leading to decreased wheat grain Cd uptake in the contaminated soil.展开更多
Plate culture counting and strain isolation methods were utilized to assess the species richness and abundance of planktonic and attached bacteria on glass plates in the surface and bottom seawater of Qingdao Middle H...Plate culture counting and strain isolation methods were utilized to assess the species richness and abundance of planktonic and attached bacteria on glass plates in the surface and bottom seawater of Qingdao Middle Harbor over a year,with monthly and quarterly sampling.Both species richness and bacterial numbers exhibited seasonal variations.Specifically,the abundance of attached bacteria and bacterioplankton peaked in June and July,corresponding to higher water temperatures in summer and autumn,while lower abundances were noted in January and December during cooler periods.Throughout the year,the species richness of attached bacteria consistently exceeded that of planktonic bacteria in both shallow and deep waters.Pseudoalteromonas emerged as the most prevalent genus among both planktonic and attached bacteria in surface and bottom seawater samples.Furthermore,the magnitude of changes in species richness and abundance for attached bacteria(0.66×10^(5)-15.85×10^(5)CFU/cm^(2))was greater than that observed for planktonic bacteria(0.58×10^(8)-5.33×10^(8)CFU/L).We propose that the attached bacterial populations,situated in limited microenvironments within the larger seawater ecosystem,exhibit heightened sensitivity to environmental fluctuations,resulting in more rapid shifts in population dynamics and lower ecological stability.The theoretical implications and potential applications of these findings warrant further investigation.展开更多
Salt-tolerant bacteria associated with halophytes enhance plant resistance and adaptation to environmental stress.The purpose of this study was to investigate the diversity and plant-beneficial traits of bacteria asso...Salt-tolerant bacteria associated with halophytes enhance plant resistance and adaptation to environmental stress.The purpose of this study was to investigate the diversity and plant-beneficial traits of bacteria associated with three halophytes in an arid land,Northwest China.The bacterial strains were isolated from the roots,shoots,rhizosphere,and bulk soil of three halophytes,i.e.,Salicornia europaea L.,Kalidium foliatum(Pall.)Moq.,and Suaeda aralocaspica(Bunge)Freitag&Schütze,collected from the saline soils near to the Wujiaqu City,Xinjiang,Northwest China.A total of 567 strains were isolated and identified from these three halophytes belonging to 4 phyla,6 classes,25 orders,36 families,and 66 genera,including 147 potential novel species.A total of 213 strains exhibited one or more plant growthpromoting properties,while 20 strains demonstrated multiple in vitro plant growth-promoting activities,including phosphate solubilization,nitrogen fixation,siderophore production,and production of hydrolytic enzymes such as protease and cellulase.Our findings showed that halophytes in the arid land harbor diverse bacteria with the potential to enhance plant growth and adaptability under challenging environmental conditions.展开更多
Rice-fish coculture(RFC)has aroused extensive concern for its contribution to food security and resource conservation,but whether it can improve soil phosphorus(P)availability and affect microbe-mediated P turnover re...Rice-fish coculture(RFC)has aroused extensive concern for its contribution to food security and resource conservation,but whether it can improve soil phosphorus(P)availability and affect microbe-mediated P turnover remains elusive.Herein,we conducted a microcosm experiment to assess the impacts of RFC combined with(50 mg P kg^(-1)as KH2PO4)and without inorganic P addition on P fractions,P availability,and phoD-harboring bacterial community composition.The results revealed that RFC without P addition significantly improved P availability and phosphatase activity in paddy soil,while soil available P(AP),pH,and microbial biomass P(MBP)contributed to regulating P fractions.Moreover,the phoD-harboring bacterial abundance was linked to phosphatase activity,AP,total carbon(TC),and total P(TP)contents,and the ratios of TC to total nitrogen(TN)and TN to TP.We also found that the keystone taxa of phoD-harboring bacteria contributed to phosphatase production as well as organic P mineralization,thereby improving P availability.Our findings suggest that RFC without P addition is beneficial for promoting the expression of phoD-harboring bacterial functions to improve the capacity of P mineralization.Overall,our study provides insights into the responses of phoD-harboring bacterial functions for P turnover to RFC combined with and without P addition,showing the potential utilization of P resources in agricultural soil and the contribution of phosphatase activity to P acquisition in agriculture ecosystem.展开更多
Intracellular bacteria(ICB),cloaked by the protective barriers of host cells,pose a formidable challenge to selective and efficient eradication.The employment of activatable photosensitizers based antibacterial photod...Intracellular bacteria(ICB),cloaked by the protective barriers of host cells,pose a formidable challenge to selective and efficient eradication.The employment of activatable photosensitizers based antibacterial photodynamic therapy(a PDT)holds significant potential for selective imaging and photo-inactivation of ICB while minimizing side effects on normal cells.Drawing inspiration from the elevated hypochlorous acid(HClO)levels in ICB infected phagocytes,herein we firstly designed and synthesized a series of HCl Oresponsive dinuclear Ru(Ⅱ)complexes(Ru1-Ru3)to achieve such a goal.Initially,the luminescence,^(1)O_(2)generation and a PDT activity of these Ru(Ⅱ)complexes were suppressed due to the quenching effect of the azo group,but were recovered after reaction with HCl O in solutions or within ICB infected phagocytes.The detailed results revealed that Ru1 and Ru3 could not only selectively visualize ICB,but also demonstrated remarkable a PDT activity against ICB,surpassing vancomycin both in vitro and in vivo.展开更多
Plant bacterial diseases have infiicted substantial economic losses in global crop,fruit,and vegetable production.The conventional methods for managing these diseases typically rely on the application of antibiotics.H...Plant bacterial diseases have infiicted substantial economic losses in global crop,fruit,and vegetable production.The conventional methods for managing these diseases typically rely on the application of antibiotics.However,these antibiotics often target the growth factors of the pathogenic bacteria,leading to the accumulation and emergence of drug-resistant strains,which exacerbates antibiotic resistance.Innovative methods are urgently needed to treat and prevent the toxicity caused by these pathogenic bacteria.Targeting virulence mechanisms in pathogens is a globally recognized and effective strategy for mitigating bacterial resistance.TypeⅢsecretion system(T3SS)serves as a crucial virulence determinant in Gram-negative pathogens,and its non-essentials for pathogen growth renders it an ideal target.Targeting the T3SS holds significant potential to alleviate selective pressure for resistance mutations in pathogens.Therefore,targeting T3SS in pathogenic bacteria,while preserving their growth,has emerged as a novel avenue for the development of antimicrobial drugs.In recent years,a multitude of small molecular inhibitors targeting T3SS have been identified.This article offers a comprehensive review of T3SS inhibitors in plant pathogens,while also presenting the latest research advancements in this research direction.展开更多
BACKGROUND: Sepsis is a life-threatening inflammatory condition in which the invading pathogen avoids the host's defense mechanisms and continuously stimulates and damages host cells. Consequently, many immune res...BACKGROUND: Sepsis is a life-threatening inflammatory condition in which the invading pathogen avoids the host's defense mechanisms and continuously stimulates and damages host cells. Consequently, many immune responses initially triggered for protection become harmful because of the failure to restore homeostasis, resulting in ongoing hyperinflammation and immunosuppression. METHODS: A literature review was conducted to address bacterial sepsis, describe advances in understanding complex immunological reactions, critically assess diagnostic approaches, and emphasize the importance of studying bacterial bottlenecks in the detection and treatment of sepsis.RESULTS: Diagnosing sepsis via a single laboratory test is not feasible;therefore, multiple key biomarkers are typically monitored, with a focus on trends rather than absolute values. The immediate interpretation of sepsis-associated clinical signs and symptoms, along with the use of specific and sensitive laboratory tests, is crucial for the survival of patients in the early stages. However, long-term mortality associated with sepsis is now recognized, and alongside the progression of this condition, there is an in vivo selection of adapted pathogens.CONCLUSION: Bacterial sepsis remains a significant cause of mortality across all ages and societies. While substantial progress has been made in understanding the immunological mechanisms underlying the inflammatory response, there is growing recognition that the ongoing host-pathogen interactions, including the emergence of adapted virulent strains, shape both the acute and long-term outcomes in sepsis. This underscores the urgent need for novel high-throughput diagnostic methods and a shift toward more pre-emptive, rather than reactive, treatment strategies in sepsis care.展开更多
Diel investigations of water environments are one means to holistically understand the dynamics and functional roles of phytoplankton,bacteria and viruses in these ecosystems.They have the potential to substantially i...Diel investigations of water environments are one means to holistically understand the dynamics and functional roles of phytoplankton,bacteria and viruses in these ecosystems.They have the potential to substantially impact carbon(C),nitrogen(N)and phosphorus(P)biogeochemistry through their respective roles.This study characterizes the phytoplankton,bacteria and virus communities and the elemental composition of various C,N and P nutrients flow over three diel cycles in tropical urban lake.Our results show that ratios of C:N:P fluctuated strongly from the lack of dissolved organic phosphorus(DOP)and PO_(4).Specifically,green algae peaked during day time and exudate dissolved organic matter(DOM)that strongly modulate dissolved organic carbon(DOC):DOP ratio to diel DOP limitation.Multiple linear regression and Stella modelling emphasize the roles of viruses together with Synechococcus as important nutrient recyclers of NH_(4)and PO_(4)in nutrients-limited waters.Respective normalised surface PO_(4)and combined surface and bottom NH_(4)concentration selected both viruses and Synechococcus as important drivers.Process model of N and P biogeochemical cycles can achieve 69%and 57%similar to observed concentration of NH_(4)and PO_(4),respectively.A short latent period of 9 hr was calculated,in addition to the calibrated high infectivity of viruses to Synechococcus.Taken together,the rapid turn-over between Synechococcus and viruses has biogeochemical significance,where the rapid recycling of essential nutrients allows for shortcuts in the N and P cycle,supporting a wide range of microbes.展开更多
Because of the recent widespread usage of antibiotics,the acquisition and dissemination of antibiotic-resistance genes(ARGs)were prevalent in the majority of habitats.Generally,the biological wastewater treatment proc...Because of the recent widespread usage of antibiotics,the acquisition and dissemination of antibiotic-resistance genes(ARGs)were prevalent in the majority of habitats.Generally,the biological wastewater treatment processes used in wastewater treatment plants have a limited efficiencies of antibiotics resistant bacteria(ARB)disinfection and ARGs degradation and even promote the proliferation of ARGs.Problematically,ARB and ARGs in effluent pose potential risks if they are not further treated.Photocatalytic oxidation is considered a promising disinfection technology,where the photocatalytic process generates many free radicals that enhance the interaction between light and deoxyribonucleic acid(DNA)for ARB elimination and subsequent degradation of ARGs.This reviewaims to illustrate the progress of photocatalytic oxidation technology for removing antibiotics resistant(AR)from wastewater in recent years.We discuss the sources and transfer of ARGs in wastewater.The overall removal efficiencies of ultraviolet radiation(UV)/chlorination,UV/ozone,UV/H_(2)O_(2),and UV/sulfate-radical based system for ARB and ARGs,as well as the experimental parameters and removal mechanisms,are systematically discussed.The contribution of photocatalytic materials based on TiO_(2) and g-C_(3)N_(4) to the inactivation of ARB and degradation of ARGs is highlighted,producingmany free radicals to attack ARB and ARGs while effectively limiting the horizontal gene transfer(HGT)in wastewater.Finally,based on the reviewed studies,future research directions are proposed to realize specific photocatalytic oxidation technology applications and overcome current challenges.展开更多
Objective: This study assessed the effects of consuming acetic acid bacteria (Gluconacetobacter hansenii GK-1) for 12 weeks on fatigue induced by temporary mental stress. Methods: This randomized, double-blind, placeb...Objective: This study assessed the effects of consuming acetic acid bacteria (Gluconacetobacter hansenii GK-1) for 12 weeks on fatigue induced by temporary mental stress. Methods: This randomized, double-blind, placebo-controlled, parallel-group study included 100 healthy male and female adults aged 20 - 64 years. Participants consumed either the G. hansenii GK-1 supplement (9 × 10⁹ cells/day) or a placebo daily for 12 weeks. The impact of temporary mental stress on fatigue in G. hansenii GK-1 was assessed using a Visual Analog Scale (VAS) before the study began and after 12 weeks of supplementation. Results: Subjective fatigue measured by Visual Analog Scale (VAS) showed a significant decrease in fatigue induced by temporary mental stress after 12 weeks of consumption in the G. hansenii GK-1 group compared with the placebo group. No adverse events were attributed to G. hansenii GK-1. These findings confirm that continuous oral ingestion of G. hansenii GK-1 by healthy Japanese adults reduces feelings of fatigue caused by temporary mental stress.展开更多
Background Coccidiosis,caused by Eimeria parasites,is a major enteric disease in poultry,significantly impacting animal health,production performance,and welfare.This disease imposes a substantial economic burden,cost...Background Coccidiosis,caused by Eimeria parasites,is a major enteric disease in poultry,significantly impacting animal health,production performance,and welfare.This disease imposes a substantial economic burden,costing the global poultry industry up to$13 billion annually.However,effective mitigation strategies for coccidiosis remain elusive.While different chicken breeds exhibit varying resistance to coccidiosis,no commensal bacteria have been directly linked to this resistance.Methods To assess relative resistance of different breeds to coccidiosis,10-day-old Fayoumi M5.1,Leghorn Ghs6,and Cobb chickens were challenged with 50,000 sporulated Eimeria maxima oocysts or mock-infected.Body weight changes,small intestinal lesions,and fecal oocyst shedding were evaluated on d 17.Ileal and cecal digesta were collected from individual animals on d 17 and subjected to microbiome analysis using 16S rRNA gene sequencing.Results Fayoumi M5.1 chickens showed the lowest growth retardation,intestinal lesion score,fecal oocyst shedding,and pathobiont proliferation compared to Ghs6 and Cobb chickens.The intestinal microbiota of M5.1 chickens also differed markedly from the other two breeds under both healthy and coccidiosis conditions.Notably,group A Lactobacillus and Ligilactobacillus salivarius were the least prevalent in both the ileum and cecum of healthy M5.1 chickens,but became highly enriched and comparable to Ghs6 and Cobb chickens in response to coccidiosis.Conversely,Weissella,Staphylococcus gallinarum,and Enterococcus durans/hirae were more abundant in the ileum of healthy M5.1 chickens than in the other two breeds.Despite being reduced by Eimeria,these bacteria retained higher abundance in M5.1 chickens compared to the other breeds.Conclusions Fayoumi M5.1 chickens exhibit greater resistance to coccidiosis than Leghorn Ghs6 layers and Cobb broilers.Several commensal bacteria,including group A Lactobacillus,L.salivarius,Weissella,S.gallinarum,and E.durans/hirae,are differentially enriched in Fayoumi M5.1 chickens with strong correlation with coccidiosis resistance.These bacteria hold potential as probiotics for coccidiosis mitigation.展开更多
This study examined the effects of pasteurization(PAS),ultrasonic sterilization(ULS),and microwave sterilization(MWS)on the quality and storage characteristics of brine-fermented tofu(BFT)and fermented tofu(FT).Compar...This study examined the effects of pasteurization(PAS),ultrasonic sterilization(ULS),and microwave sterilization(MWS)on the quality and storage characteristics of brine-fermented tofu(BFT)and fermented tofu(FT).Comparative analysis revealed that MWS had a negligible detrimental effect on the structural integrity and organoleptic properties of BFT and FT,while effectively maintaining its water-holding capacity(WHC)and exhibiting the least impact on its texture.In contrast,PAS and ULS increased hardness and chewiness significantly(P<0.05),but ULS also enhanced the brightness of tofu.Throughout the storage period,the WHC,elasticity,and sensory properties of tofu generally decreased,whereas the hardness and chewiness increased.PAS-BFT and MWS-FT maintained sensory quality for the longest periods of 14 and 12 days respectively,and could be decomposed to more small molecule peptides within 0–8 days and 0–6 days,which are more easily to be absorbed by the body.The findings discovered that MWS is the most suitable method for sterilization of tofu,with superior capability in maintaining the quality,extending shelf life,and improving digestibility of tofu.展开更多
Deep learning neural network incorporating surface enhancement Raman scattering technique(SERS)is becoming as a powerful tool for the precise classifications and diagnosis of bacterial infections.However,the large amo...Deep learning neural network incorporating surface enhancement Raman scattering technique(SERS)is becoming as a powerful tool for the precise classifications and diagnosis of bacterial infections.However,the large amount of sample requirement and time-consuming sample collection severely hinder its applications.We herein propose a spectral concatenation strategy for residual neural network using nonspecific and specific SERS spectra for the training data augmentation,which is accessible to acquiring larger training dataset with same number of SERS spectra or same size of training dataset with fewer SERS spectra,compared with pure non-specific SERS spectra.With this strategy,the training loss exhibit rapid convergence,and an average accuracy up to 100%in bacteria classifications was achieved with50 SERS spectra for each kind of bacterium;even reduced to 20 SERS spectra per kind of bacterium,classification accuracy is still>95%,demonstrating marked advantage over the results without spectra concatenation.This method can markedly improve the classification accuracy under fewer samples and reduce the data collection workload,and can evidently enhance the performance when used in different machine learning models with high generalization ability.Therefore,this strategy is beneficial for rapid and accurate bacteria classifications with residual neural network.展开更多
Mariculture tailwater poses significant environmental challenges such as water pollution and eutrophication.Photosynthetic bacteria(PSB)have been widely used for pollutant treatment.The integrated aquaculture wastewat...Mariculture tailwater poses significant environmental challenges such as water pollution and eutrophication.Photosynthetic bacteria(PSB)have been widely used for pollutant treatment.The integrated aquaculture wastewater bioremediation system(IAWBS)consists of four key components:sedimentation,biofilm,shellfish,and macro-algae.However,there is still some room for improvement in the treatment efficiency of the IAWBS.In this study,PSB(Rhodopseudomonos,Rhodobacteria,Rhodococcus)were added to the system.High-throughput amplicon of 16S rRNA,PERMANOVA,NMDS and other statistical analyses were used to investigate the impacts of PSB.The results showed that significant removal efficiencies of CODMn,NH_(4)^(+)-N,NO_(3)^(-)-N,NO_(2)^(-)-N,PO_(4)^(3-)-P were 71.42%,91.37%,91.72%,87.20%,and 88.80%,respectively.The impact of PSB on bacterioplankton community(BC)was not affected by the time interval but affected by different units,Proteobacteria,Bacteriodia and Actinobacteria were the most abundant phyla in all the units.Alpha diversity underscored a significant decrease in bacterial community diversity due to the PSB.Notably,the PERMANOVA analysis highlighted a significant interaction between the time interval and treated unit,influencing the composition of the microbial community(R^(2)=0.152;P=0.001).This study provides a better understanding of the role of photosynthetic bacteria in an integrated bioremediation system that can effectively treat mariculture tailwater,which is of great significance for the sustainable development of aquaculture.展开更多
Both the broad-spectrum bactericidal properties of disinfectants and the inhibitory effects of antibiotics pose potential threats to the activity,functionality,and probiotic properties of Lactid acid bacteria(LAB).The...Both the broad-spectrum bactericidal properties of disinfectants and the inhibitory effects of antibiotics pose potential threats to the activity,functionality,and probiotic properties of Lactid acid bacteria(LAB).Therefore,it is essential to systematically evaluate the tolerance of lactic acid bacteria to common disinfectants and antibiotics.This study assessed 60 LAB strains isolated from the fermented foods for their tolerance to seven disinfectants and nine antibiotics.The results indicated that,compared to pathogenic bacteria,most LAB exhibited stronger tolerance to disinfectants.Sub-inhibitory concentrations of disinfectants inhibited biofilm formation in most LAB,while sub-inhibitory concentrations of glutaraldehyde tended to promote biofilm formation.This suggests that commonly used disinfectants can eliminate pathogenic bacteria without killing LAB,providing guidance for disinfectant use.LAB were highly sensitive to ampicillin and chloramphenicol,while showing some tolerance to norfloxacin,polymyxin,and sulfamethoxazole.This indicates LAB have a notable antibiotic resistance profile,which includes both natural resistance characteristics and instances of acquired resistance,along with multiple drug resistance and cross-resistance between antibiotics and disinfectants.This study provides an important guidance for disinfection practices in industrial utilizing LAB,such as food and biomedicine,and offers a reference for the use of antibiotics.展开更多
The Makran Sea is a complex marine environment.The purpose of this research is screening of emulsifier-producing bacteria in this marine environment and optimization of emulsifier production by the best-producing stra...The Makran Sea is a complex marine environment.The purpose of this research is screening of emulsifier-producing bacteria in this marine environment and optimization of emulsifier production by the best-producing strain.Marine samples(seawater and sediments)were collected from four different zones in the Makran Sea.The emulsification activity index(E_(24))and Bacterial Ad-hesion To Hydrocarbons(BATH)were used to select the best emulsifier-producing strains.The prevalent strains were identified by PCR.The optimization of the emulsifier production medium by the best strain was done by two-level factorial design.Seventeen emulsifier-producing strains were isolated from sediments and seawater in the Makran Sea.The strains M6,BS-2,and J6 were select-ed between all isolates because they have 83%,91%,and 85%emulsification activity(E_(24))respectively.The results of sequencing confirmed that these three strains(M6,BS-2,and J6)belong to Cobetia marina,Shewanella alga,and Thalassospira permensis re-spectively.Maximum emulsifier production occurred at crude oil concentration(4%,v/v),peptone(2.5 g/L),yeast extract(1.5 g/L),molasses(2%),and at a temperature of 25℃.The results of this research confirmed that the Makran Sea has the potential to reach ro-bust marine bacteria with different biotechnological applications.展开更多
This article discusses the original article published by Lu et al in the latest issue.The article confirmed through a cohort study the clinical efficacy of the triple live bacteria of Bifidobacterium combined with mir...This article discusses the original article published by Lu et al in the latest issue.The article confirmed through a cohort study the clinical efficacy of the triple live bacteria of Bifidobacterium combined with mirtazapine in treating postoperative depression of gastric cancer patients,also explored the multi-target therapeutic mechanisms of Bifidobacterium triple viable bacteria combined with mirtazapine in managing depression in patients after radical gastrectomy for gastric cancer for the first time.The results indicated that Bifidobacterium could alleviate depression in patients after radical gastrectomy for gastric cancer.Gastric cancer patients undergoing surgery frequently experience postoperative psychological disorders,notably depression.These conditions not only impair quality of life but also negatively impact disease-free and overall survival.This editorial explores the underestimated psychological challenges following gastric cancer surgery,including depression,anxiety,adjustment disorders,and post-traumatic stress disorder.It further examines the therapeutic potential of Bifidobacterium triple viable probiotics,combined with mirtazapine,in alleviating postoperative depression through microbiological,immunological,and neurological mechanisms.Clinical evidence highlights additional benefits,such as improved gastrointestinal function,enhanced immunity,and better nutritional status,underscoring a holistic approach to postoperative care.While promising,further large-scale trials are needed to optimize and personalize probiotic-based interventions.展开更多
Oryza longistaminata is an African wild rice species with valuable agronomic traits and the donor parent of perennial rice.Endophytic bacteria play an important role in host health,adaptive evolution and stress tolera...Oryza longistaminata is an African wild rice species with valuable agronomic traits and the donor parent of perennial rice.Endophytic bacteria play an important role in host health,adaptive evolution and stress tolerance.However,endophytic bacterial communities in O.longistaminata and their plant growth-promoting(PGP)effects on the perennial rice of O.longistaminata offspring are poorly understood.In this study,the endophytic bacterial diversity,composition and network structures in the root,stem,and leaf tissues of O.longistaminata were characterized using Illumina sequencing of the 16S rRNA gene.The results suggested that O.longistaminata contains a multitude of niches for different endophytic bacteria,among which the root endosphere is more complex and functionally diverse than the stem and leaf endospheres.Tissue-specific biomarkers were identified,including Paludibaculum,Pseudactinotalea and Roseimarinus and others,for roots,Blautia for stems and Lachnospiraceae NK4A136 for leaves.The endophytic bacterial network of O.longistaminata was reassembled for various functions,including degradation/utilization/assimilation,detoxification,generation of precursor metabolites and energy,glycan pathways,macromolecule modification and metabolism.A total of 163 endophytic bacterial strains with PGP traits of potassium release,phosphate solubilization,nitrogen fixation,siderophore activity,indole-3-acetic acid(IAA)production,and 1-aminocyclopropane-1-carboxylate(ACC)deaminase activity were isolated from O.longistaminata.Eleven strains identified as Enterobacter cloacae,Enterobacter ludwigii,Stenotrophomonas maltophilia,Serratia fonticola,and Bacillus velezensis showed stable colonization abilities and PGP effects on perennial rice seedlings.Inoculated plants generally exhibited an enhanced root system and greater photosynthesis,biomass accumulation and nutrient uptake.Interestingly,two strains of E.cloacae have host genotype-dependent effects on perennial rice growth.The results of this study provide insights into the endophytic bacterial ecosystems of O.longistaminata,which can potentially be used as biofertilizers for sustainable perennial rice productivity.展开更多
This study investigates the compatibility and efficacy of combining ammonium molybdate(AM)with antagonistic bacteria Bacillus amyloliquefaciens B10W10 and Pseudomonas sp.B11W11 for brown rot control(Monilinia laxa).In...This study investigates the compatibility and efficacy of combining ammonium molybdate(AM)with antagonistic bacteria Bacillus amyloliquefaciens B10W10 and Pseudomonas sp.B11W11 for brown rot control(Monilinia laxa).In vitro experiments reveal variable mycelial growth inhibition rates compared to untreated controls,with B11W11+0.5%AM and B10W10+2%AM displaying the highest inhibition rates after 5 days.After 10 days,the 2%AM+B10W10 combination exhibits the highest inhibition rate.Microscopic observations show structural alterations in mycelium within inhibition zones,marked by vacuolization.The antagonistic bacteria,alone or with different ammonium molybdate concentrations,significantly impact M.laxa spore germination,with the B10W10 cell filtrate+2%ammonium molybdate combination achieving the most substantial inhibition.Conversely,the 0.5%ammonium molybdate treatment has the lowest inhibition rate while the combination of AM and bacteria is giving better results compared to the use of bacteria alone.Fruits treated with various antagonistic bacteria and ammonium molybdate combinations demonstrate a significant reduction in disease severity.The 0.5%AM+B10W10 combination exhibits the lowest severity.FT-IR spectra analysis identifies shifts in fungal biomass functional groups,with reduced lignin-related bands and increased phenols,lipids,polysaccharides,and carbohydrates.This highlights the structural modifications caused by the biological treatments.The study also evaluates the effects on fruit quality parameters.The 2%ammonium molybdate treatment yields the lowest weight loss.TSS levels are affected by salt concentration,while acid content remains consistent across treatments.All treatments influence fruit firmness compared to controls.These findings emphasize the potential of combining ammonium molybdate and antagonistic bacteria for effective brown rot control,highlighting their compatibility and effects on disease severity,fungal biomass,spore germination,and fruit quality.展开更多
Soil microbial communities are key factors in maintaining ecosystem multifunctionality(EMF).However,the distribution patterns of bacterial diversity and how the different bacterial taxa and their diversity dimensions ...Soil microbial communities are key factors in maintaining ecosystem multifunctionality(EMF).However,the distribution patterns of bacterial diversity and how the different bacterial taxa and their diversity dimensions affect EMF remain largely unknown.Here,we investigated variation in three measures of diversity(alpha diversity,community composition and network complexity)among rare,intermediate,and abundant taxa across a latitudinal gradient spanning five forest plots in Yunnan Province,China and examined their contributions on EMF.We aimed to characterize the diversity distributions of bacterial groups across latitudes and to assess the differences in the mechanisms underlying their contributions to EMF.We found that multifaceted diversity(i.e.,diversity assessed by the three different metrics)of rare,intermediate,and abundant bacteria generally decreased with increasing latitude.More importantly,we found that rare bacterial taxa tended to be more diverse,but they contributed less to EMF than intermediate or abundant bacteria.Among the three dimensions of diversity we assessed,only community composition significantly affected EMF across all locations,while alpha diversity had a negative effect,and network complexity showed no significant impact.Our study further emphasizes the importance of intermediate and abundant bacterial taxa as well as community composition to EMF and provides a theoretical basis for investigating the mechanisms by which belowground microorganisms drive EMF along a latitudinal gradient.展开更多
基金supported by the National Natural Science Foundation of China(No.41977199).
文摘In this study,two wheat-derived cadmium(Cd)-immobilizing endophytic Pseudomonas paralactis M14 and Priestia megaterium R27 were evaluated for their effects on wheat tissue Cd uptake under hydroponic conditions.Then,the impacts of the biochar(BC),M14+R27(MR),and BC+MR treatments on wheat Cd uptake and the mechanisms involved were investigated at the jointing,heading,and mature stages of wheat plants under field-plot conditions.A hydroponic experiment showed that the MR treatment significantly decreased the above-ground tissue Cd content compared with theM14 or R27 treatment.The BC+MRtreatment reduced the grain Cd content by 51.5%-67.7%and Cd translocation factor at the mature stage of wheat plants and increased the organic matter-bound Cd content by 31%-75%in the rhizosphere soils compared with the BC or MR treatment.Compared with the BC or MR treatment,the relative abundances of the biomarkers associated with Gemmatimonas,Altererythrobacter,Gammaproteobacteria,Xanthomonadaceae,Phenylobacterium,and Nocardioides in the BC+MR-treated rhizosphere microbiome decreased and negatively correlated with the organic matter-bound Cd contents.In the BC+MR-treated root interior microbiome,the relative abundance of the biomarker belonging to Exiguobacterium increased and negatively correlated with the Cd translocation factor,while the relative abundance of the biomarker belonging to Pseudonocardiaceae decreased and positively correlated with the Cd translocation factor.Our findings suggested that the BC+MR treatment reduced Cd availability and Cd transfer through affecting the abundances of these specific biomarkers in the rhizosphere soil and root interior microbiomes,leading to decreased wheat grain Cd uptake in the contaminated soil.
基金The National Natural Science Foundation of China under contract Nos 42206126 and 42076044the Natural Science Foundation of Shandong Province under contract No.ZR2021QD099.
文摘Plate culture counting and strain isolation methods were utilized to assess the species richness and abundance of planktonic and attached bacteria on glass plates in the surface and bottom seawater of Qingdao Middle Harbor over a year,with monthly and quarterly sampling.Both species richness and bacterial numbers exhibited seasonal variations.Specifically,the abundance of attached bacteria and bacterioplankton peaked in June and July,corresponding to higher water temperatures in summer and autumn,while lower abundances were noted in January and December during cooler periods.Throughout the year,the species richness of attached bacteria consistently exceeded that of planktonic bacteria in both shallow and deep waters.Pseudoalteromonas emerged as the most prevalent genus among both planktonic and attached bacteria in surface and bottom seawater samples.Furthermore,the magnitude of changes in species richness and abundance for attached bacteria(0.66×10^(5)-15.85×10^(5)CFU/cm^(2))was greater than that observed for planktonic bacteria(0.58×10^(8)-5.33×10^(8)CFU/L).We propose that the attached bacterial populations,situated in limited microenvironments within the larger seawater ecosystem,exhibit heightened sensitivity to environmental fluctuations,resulting in more rapid shifts in population dynamics and lower ecological stability.The theoretical implications and potential applications of these findings warrant further investigation.
基金funded by the Key Research and Development Project of Xinjiang Uygur Autonomous Region (2024B02015-3)the Regional Coordinated Innovation Project (Shanghai Cooperation Organization Science and Technology Partnership Program) of Xinjiang Uygur Autonomous Region (2025E01024)
文摘Salt-tolerant bacteria associated with halophytes enhance plant resistance and adaptation to environmental stress.The purpose of this study was to investigate the diversity and plant-beneficial traits of bacteria associated with three halophytes in an arid land,Northwest China.The bacterial strains were isolated from the roots,shoots,rhizosphere,and bulk soil of three halophytes,i.e.,Salicornia europaea L.,Kalidium foliatum(Pall.)Moq.,and Suaeda aralocaspica(Bunge)Freitag&Schütze,collected from the saline soils near to the Wujiaqu City,Xinjiang,Northwest China.A total of 567 strains were isolated and identified from these three halophytes belonging to 4 phyla,6 classes,25 orders,36 families,and 66 genera,including 147 potential novel species.A total of 213 strains exhibited one or more plant growthpromoting properties,while 20 strains demonstrated multiple in vitro plant growth-promoting activities,including phosphate solubilization,nitrogen fixation,siderophore production,and production of hydrolytic enzymes such as protease and cellulase.Our findings showed that halophytes in the arid land harbor diverse bacteria with the potential to enhance plant growth and adaptability under challenging environmental conditions.
基金supported by the Key-Area Research and Development Program of Guangdong Province,China(No.2021B0202030002)the Science and Technology Planning Project of Guangdong Province,China(No.2019B030301007)+2 种基金the Guangdong Provincial Special Project of Rural Revitalization Strategy,China(No.(2021)12)the Joint Team Project of Guangdong Laboratory for Lingnan Modern Agriculture,China(No.NT2021010)the Innovation Team Construction Project of Modern Agricultural Industry Technology Systems of Guangdong Province,China(No.2022KJ105).
文摘Rice-fish coculture(RFC)has aroused extensive concern for its contribution to food security and resource conservation,but whether it can improve soil phosphorus(P)availability and affect microbe-mediated P turnover remains elusive.Herein,we conducted a microcosm experiment to assess the impacts of RFC combined with(50 mg P kg^(-1)as KH2PO4)and without inorganic P addition on P fractions,P availability,and phoD-harboring bacterial community composition.The results revealed that RFC without P addition significantly improved P availability and phosphatase activity in paddy soil,while soil available P(AP),pH,and microbial biomass P(MBP)contributed to regulating P fractions.Moreover,the phoD-harboring bacterial abundance was linked to phosphatase activity,AP,total carbon(TC),and total P(TP)contents,and the ratios of TC to total nitrogen(TN)and TN to TP.We also found that the keystone taxa of phoD-harboring bacteria contributed to phosphatase production as well as organic P mineralization,thereby improving P availability.Our findings suggest that RFC without P addition is beneficial for promoting the expression of phoD-harboring bacterial functions to improve the capacity of P mineralization.Overall,our study provides insights into the responses of phoD-harboring bacterial functions for P turnover to RFC combined with and without P addition,showing the potential utilization of P resources in agricultural soil and the contribution of phosphatase activity to P acquisition in agriculture ecosystem.
基金supported by National Natural Science Foundation of China(No.22371289)。
文摘Intracellular bacteria(ICB),cloaked by the protective barriers of host cells,pose a formidable challenge to selective and efficient eradication.The employment of activatable photosensitizers based antibacterial photodynamic therapy(a PDT)holds significant potential for selective imaging and photo-inactivation of ICB while minimizing side effects on normal cells.Drawing inspiration from the elevated hypochlorous acid(HClO)levels in ICB infected phagocytes,herein we firstly designed and synthesized a series of HCl Oresponsive dinuclear Ru(Ⅱ)complexes(Ru1-Ru3)to achieve such a goal.Initially,the luminescence,^(1)O_(2)generation and a PDT activity of these Ru(Ⅱ)complexes were suppressed due to the quenching effect of the azo group,but were recovered after reaction with HCl O in solutions or within ICB infected phagocytes.The detailed results revealed that Ru1 and Ru3 could not only selectively visualize ICB,but also demonstrated remarkable a PDT activity against ICB,surpassing vancomycin both in vitro and in vivo.
基金the financial support from the National Key Research and Development Program of China(No.2023YFD1701100)the National Natural Science Foundation of China(No.32072450)+2 种基金the National Science Fund for Distinguished Young Scholars of Guangdong Province(No.2021B1515020107)the Opening Foundation of Hubei Key Laboratory of Novel Reactor and Green Chemical Technology(No.NRG202306)the Opening Foundation of Guangdong Province Key Laboratory of Microbial Signals and Disease Control(No.MSDC2023-19)。
文摘Plant bacterial diseases have infiicted substantial economic losses in global crop,fruit,and vegetable production.The conventional methods for managing these diseases typically rely on the application of antibiotics.However,these antibiotics often target the growth factors of the pathogenic bacteria,leading to the accumulation and emergence of drug-resistant strains,which exacerbates antibiotic resistance.Innovative methods are urgently needed to treat and prevent the toxicity caused by these pathogenic bacteria.Targeting virulence mechanisms in pathogens is a globally recognized and effective strategy for mitigating bacterial resistance.TypeⅢsecretion system(T3SS)serves as a crucial virulence determinant in Gram-negative pathogens,and its non-essentials for pathogen growth renders it an ideal target.Targeting the T3SS holds significant potential to alleviate selective pressure for resistance mutations in pathogens.Therefore,targeting T3SS in pathogenic bacteria,while preserving their growth,has emerged as a novel avenue for the development of antimicrobial drugs.In recent years,a multitude of small molecular inhibitors targeting T3SS have been identified.This article offers a comprehensive review of T3SS inhibitors in plant pathogens,while also presenting the latest research advancements in this research direction.
基金funded by the Deanship of Scientific Research (DSR) at King Abdulaziz UniversityJeddah+1 种基金Saudi Arabiaunder grant number G-150-248-1443。
文摘BACKGROUND: Sepsis is a life-threatening inflammatory condition in which the invading pathogen avoids the host's defense mechanisms and continuously stimulates and damages host cells. Consequently, many immune responses initially triggered for protection become harmful because of the failure to restore homeostasis, resulting in ongoing hyperinflammation and immunosuppression. METHODS: A literature review was conducted to address bacterial sepsis, describe advances in understanding complex immunological reactions, critically assess diagnostic approaches, and emphasize the importance of studying bacterial bottlenecks in the detection and treatment of sepsis.RESULTS: Diagnosing sepsis via a single laboratory test is not feasible;therefore, multiple key biomarkers are typically monitored, with a focus on trends rather than absolute values. The immediate interpretation of sepsis-associated clinical signs and symptoms, along with the use of specific and sensitive laboratory tests, is crucial for the survival of patients in the early stages. However, long-term mortality associated with sepsis is now recognized, and alongside the progression of this condition, there is an in vivo selection of adapted pathogens.CONCLUSION: Bacterial sepsis remains a significant cause of mortality across all ages and societies. While substantial progress has been made in understanding the immunological mechanisms underlying the inflammatory response, there is growing recognition that the ongoing host-pathogen interactions, including the emergence of adapted virulent strains, shape both the acute and long-term outcomes in sepsis. This underscores the urgent need for novel high-throughput diagnostic methods and a shift toward more pre-emptive, rather than reactive, treatment strategies in sepsis care.
文摘Diel investigations of water environments are one means to holistically understand the dynamics and functional roles of phytoplankton,bacteria and viruses in these ecosystems.They have the potential to substantially impact carbon(C),nitrogen(N)and phosphorus(P)biogeochemistry through their respective roles.This study characterizes the phytoplankton,bacteria and virus communities and the elemental composition of various C,N and P nutrients flow over three diel cycles in tropical urban lake.Our results show that ratios of C:N:P fluctuated strongly from the lack of dissolved organic phosphorus(DOP)and PO_(4).Specifically,green algae peaked during day time and exudate dissolved organic matter(DOM)that strongly modulate dissolved organic carbon(DOC):DOP ratio to diel DOP limitation.Multiple linear regression and Stella modelling emphasize the roles of viruses together with Synechococcus as important nutrient recyclers of NH_(4)and PO_(4)in nutrients-limited waters.Respective normalised surface PO_(4)and combined surface and bottom NH_(4)concentration selected both viruses and Synechococcus as important drivers.Process model of N and P biogeochemical cycles can achieve 69%and 57%similar to observed concentration of NH_(4)and PO_(4),respectively.A short latent period of 9 hr was calculated,in addition to the calibrated high infectivity of viruses to Synechococcus.Taken together,the rapid turn-over between Synechococcus and viruses has biogeochemical significance,where the rapid recycling of essential nutrients allows for shortcuts in the N and P cycle,supporting a wide range of microbes.
基金supported by the National Natural Science Foundation of China (Nos.52100182 and 52300204)the the Science and Technology Innovation Program of Hunan Province (No.2023RC3122).
文摘Because of the recent widespread usage of antibiotics,the acquisition and dissemination of antibiotic-resistance genes(ARGs)were prevalent in the majority of habitats.Generally,the biological wastewater treatment processes used in wastewater treatment plants have a limited efficiencies of antibiotics resistant bacteria(ARB)disinfection and ARGs degradation and even promote the proliferation of ARGs.Problematically,ARB and ARGs in effluent pose potential risks if they are not further treated.Photocatalytic oxidation is considered a promising disinfection technology,where the photocatalytic process generates many free radicals that enhance the interaction between light and deoxyribonucleic acid(DNA)for ARB elimination and subsequent degradation of ARGs.This reviewaims to illustrate the progress of photocatalytic oxidation technology for removing antibiotics resistant(AR)from wastewater in recent years.We discuss the sources and transfer of ARGs in wastewater.The overall removal efficiencies of ultraviolet radiation(UV)/chlorination,UV/ozone,UV/H_(2)O_(2),and UV/sulfate-radical based system for ARB and ARGs,as well as the experimental parameters and removal mechanisms,are systematically discussed.The contribution of photocatalytic materials based on TiO_(2) and g-C_(3)N_(4) to the inactivation of ARB and degradation of ARGs is highlighted,producingmany free radicals to attack ARB and ARGs while effectively limiting the horizontal gene transfer(HGT)in wastewater.Finally,based on the reviewed studies,future research directions are proposed to realize specific photocatalytic oxidation technology applications and overcome current challenges.
文摘Objective: This study assessed the effects of consuming acetic acid bacteria (Gluconacetobacter hansenii GK-1) for 12 weeks on fatigue induced by temporary mental stress. Methods: This randomized, double-blind, placebo-controlled, parallel-group study included 100 healthy male and female adults aged 20 - 64 years. Participants consumed either the G. hansenii GK-1 supplement (9 × 10⁹ cells/day) or a placebo daily for 12 weeks. The impact of temporary mental stress on fatigue in G. hansenii GK-1 was assessed using a Visual Analog Scale (VAS) before the study began and after 12 weeks of supplementation. Results: Subjective fatigue measured by Visual Analog Scale (VAS) showed a significant decrease in fatigue induced by temporary mental stress after 12 weeks of consumption in the G. hansenii GK-1 group compared with the placebo group. No adverse events were attributed to G. hansenii GK-1. These findings confirm that continuous oral ingestion of G. hansenii GK-1 by healthy Japanese adults reduces feelings of fatigue caused by temporary mental stress.
基金funded by the USDA National Institute of Food and Agriculture grants(2022-67016-37208 and 2024-67016-42415)the Ralph F.and Leila W.Boulware Endowment Fund,Oklahoma Agricultural Experiment Station Project H-3268+1 种基金Iowa Agriculture and Home Economics Experiment Station Project IOW05620supported by two separate USDA National Institute of Food and Agriculture Predoctoral Fellowship grants(2024-67011-42944 and 2021-67034-35184).
文摘Background Coccidiosis,caused by Eimeria parasites,is a major enteric disease in poultry,significantly impacting animal health,production performance,and welfare.This disease imposes a substantial economic burden,costing the global poultry industry up to$13 billion annually.However,effective mitigation strategies for coccidiosis remain elusive.While different chicken breeds exhibit varying resistance to coccidiosis,no commensal bacteria have been directly linked to this resistance.Methods To assess relative resistance of different breeds to coccidiosis,10-day-old Fayoumi M5.1,Leghorn Ghs6,and Cobb chickens were challenged with 50,000 sporulated Eimeria maxima oocysts or mock-infected.Body weight changes,small intestinal lesions,and fecal oocyst shedding were evaluated on d 17.Ileal and cecal digesta were collected from individual animals on d 17 and subjected to microbiome analysis using 16S rRNA gene sequencing.Results Fayoumi M5.1 chickens showed the lowest growth retardation,intestinal lesion score,fecal oocyst shedding,and pathobiont proliferation compared to Ghs6 and Cobb chickens.The intestinal microbiota of M5.1 chickens also differed markedly from the other two breeds under both healthy and coccidiosis conditions.Notably,group A Lactobacillus and Ligilactobacillus salivarius were the least prevalent in both the ileum and cecum of healthy M5.1 chickens,but became highly enriched and comparable to Ghs6 and Cobb chickens in response to coccidiosis.Conversely,Weissella,Staphylococcus gallinarum,and Enterococcus durans/hirae were more abundant in the ileum of healthy M5.1 chickens than in the other two breeds.Despite being reduced by Eimeria,these bacteria retained higher abundance in M5.1 chickens compared to the other breeds.Conclusions Fayoumi M5.1 chickens exhibit greater resistance to coccidiosis than Leghorn Ghs6 layers and Cobb broilers.Several commensal bacteria,including group A Lactobacillus,L.salivarius,Weissella,S.gallinarum,and E.durans/hirae,are differentially enriched in Fayoumi M5.1 chickens with strong correlation with coccidiosis resistance.These bacteria hold potential as probiotics for coccidiosis mitigation.
基金supported by the Innovation Talents Project of Harbin Science and Technology Bureau(2022CXRCCGO11)。
文摘This study examined the effects of pasteurization(PAS),ultrasonic sterilization(ULS),and microwave sterilization(MWS)on the quality and storage characteristics of brine-fermented tofu(BFT)and fermented tofu(FT).Comparative analysis revealed that MWS had a negligible detrimental effect on the structural integrity and organoleptic properties of BFT and FT,while effectively maintaining its water-holding capacity(WHC)and exhibiting the least impact on its texture.In contrast,PAS and ULS increased hardness and chewiness significantly(P<0.05),but ULS also enhanced the brightness of tofu.Throughout the storage period,the WHC,elasticity,and sensory properties of tofu generally decreased,whereas the hardness and chewiness increased.PAS-BFT and MWS-FT maintained sensory quality for the longest periods of 14 and 12 days respectively,and could be decomposed to more small molecule peptides within 0–8 days and 0–6 days,which are more easily to be absorbed by the body.The findings discovered that MWS is the most suitable method for sterilization of tofu,with superior capability in maintaining the quality,extending shelf life,and improving digestibility of tofu.
基金supported by the National Key Research and Development Program of China(No.2023YFC3402900)the National Nature Science of Foundation(No.61875131)+1 种基金Shenzhen Key Laboratory of Photonics and Biophotonics(No.ZDSYS20210623092006020)Shenzhen Science and Technology Innovation Program(No.20231120175730001)。
文摘Deep learning neural network incorporating surface enhancement Raman scattering technique(SERS)is becoming as a powerful tool for the precise classifications and diagnosis of bacterial infections.However,the large amount of sample requirement and time-consuming sample collection severely hinder its applications.We herein propose a spectral concatenation strategy for residual neural network using nonspecific and specific SERS spectra for the training data augmentation,which is accessible to acquiring larger training dataset with same number of SERS spectra or same size of training dataset with fewer SERS spectra,compared with pure non-specific SERS spectra.With this strategy,the training loss exhibit rapid convergence,and an average accuracy up to 100%in bacteria classifications was achieved with50 SERS spectra for each kind of bacterium;even reduced to 20 SERS spectra per kind of bacterium,classification accuracy is still>95%,demonstrating marked advantage over the results without spectra concatenation.This method can markedly improve the classification accuracy under fewer samples and reduce the data collection workload,and can evidently enhance the performance when used in different machine learning models with high generalization ability.Therefore,this strategy is beneficial for rapid and accurate bacteria classifications with residual neural network.
基金financially supported by the National Key R&D Program of China(No.2020YFD0900201)the Ningbo Public Welfare Technology Application Research Project(No.2022S164)the K.C.Wong Magna Fund in Ningbo University。
文摘Mariculture tailwater poses significant environmental challenges such as water pollution and eutrophication.Photosynthetic bacteria(PSB)have been widely used for pollutant treatment.The integrated aquaculture wastewater bioremediation system(IAWBS)consists of four key components:sedimentation,biofilm,shellfish,and macro-algae.However,there is still some room for improvement in the treatment efficiency of the IAWBS.In this study,PSB(Rhodopseudomonos,Rhodobacteria,Rhodococcus)were added to the system.High-throughput amplicon of 16S rRNA,PERMANOVA,NMDS and other statistical analyses were used to investigate the impacts of PSB.The results showed that significant removal efficiencies of CODMn,NH_(4)^(+)-N,NO_(3)^(-)-N,NO_(2)^(-)-N,PO_(4)^(3-)-P were 71.42%,91.37%,91.72%,87.20%,and 88.80%,respectively.The impact of PSB on bacterioplankton community(BC)was not affected by the time interval but affected by different units,Proteobacteria,Bacteriodia and Actinobacteria were the most abundant phyla in all the units.Alpha diversity underscored a significant decrease in bacterial community diversity due to the PSB.Notably,the PERMANOVA analysis highlighted a significant interaction between the time interval and treated unit,influencing the composition of the microbial community(R^(2)=0.152;P=0.001).This study provides a better understanding of the role of photosynthetic bacteria in an integrated bioremediation system that can effectively treat mariculture tailwater,which is of great significance for the sustainable development of aquaculture.
基金supported by the National Science Foundation of China (32001663)。
文摘Both the broad-spectrum bactericidal properties of disinfectants and the inhibitory effects of antibiotics pose potential threats to the activity,functionality,and probiotic properties of Lactid acid bacteria(LAB).Therefore,it is essential to systematically evaluate the tolerance of lactic acid bacteria to common disinfectants and antibiotics.This study assessed 60 LAB strains isolated from the fermented foods for their tolerance to seven disinfectants and nine antibiotics.The results indicated that,compared to pathogenic bacteria,most LAB exhibited stronger tolerance to disinfectants.Sub-inhibitory concentrations of disinfectants inhibited biofilm formation in most LAB,while sub-inhibitory concentrations of glutaraldehyde tended to promote biofilm formation.This suggests that commonly used disinfectants can eliminate pathogenic bacteria without killing LAB,providing guidance for disinfectant use.LAB were highly sensitive to ampicillin and chloramphenicol,while showing some tolerance to norfloxacin,polymyxin,and sulfamethoxazole.This indicates LAB have a notable antibiotic resistance profile,which includes both natural resistance characteristics and instances of acquired resistance,along with multiple drug resistance and cross-resistance between antibiotics and disinfectants.This study provides an important guidance for disinfection practices in industrial utilizing LAB,such as food and biomedicine,and offers a reference for the use of antibiotics.
基金supported by Shahid Bahonar Universi-ty of Kerman.
文摘The Makran Sea is a complex marine environment.The purpose of this research is screening of emulsifier-producing bacteria in this marine environment and optimization of emulsifier production by the best-producing strain.Marine samples(seawater and sediments)were collected from four different zones in the Makran Sea.The emulsification activity index(E_(24))and Bacterial Ad-hesion To Hydrocarbons(BATH)were used to select the best emulsifier-producing strains.The prevalent strains were identified by PCR.The optimization of the emulsifier production medium by the best strain was done by two-level factorial design.Seventeen emulsifier-producing strains were isolated from sediments and seawater in the Makran Sea.The strains M6,BS-2,and J6 were select-ed between all isolates because they have 83%,91%,and 85%emulsification activity(E_(24))respectively.The results of sequencing confirmed that these three strains(M6,BS-2,and J6)belong to Cobetia marina,Shewanella alga,and Thalassospira permensis re-spectively.Maximum emulsifier production occurred at crude oil concentration(4%,v/v),peptone(2.5 g/L),yeast extract(1.5 g/L),molasses(2%),and at a temperature of 25℃.The results of this research confirmed that the Makran Sea has the potential to reach ro-bust marine bacteria with different biotechnological applications.
文摘This article discusses the original article published by Lu et al in the latest issue.The article confirmed through a cohort study the clinical efficacy of the triple live bacteria of Bifidobacterium combined with mirtazapine in treating postoperative depression of gastric cancer patients,also explored the multi-target therapeutic mechanisms of Bifidobacterium triple viable bacteria combined with mirtazapine in managing depression in patients after radical gastrectomy for gastric cancer for the first time.The results indicated that Bifidobacterium could alleviate depression in patients after radical gastrectomy for gastric cancer.Gastric cancer patients undergoing surgery frequently experience postoperative psychological disorders,notably depression.These conditions not only impair quality of life but also negatively impact disease-free and overall survival.This editorial explores the underestimated psychological challenges following gastric cancer surgery,including depression,anxiety,adjustment disorders,and post-traumatic stress disorder.It further examines the therapeutic potential of Bifidobacterium triple viable probiotics,combined with mirtazapine,in alleviating postoperative depression through microbiological,immunological,and neurological mechanisms.Clinical evidence highlights additional benefits,such as improved gastrointestinal function,enhanced immunity,and better nutritional status,underscoring a holistic approach to postoperative care.While promising,further large-scale trials are needed to optimize and personalize probiotic-based interventions.
基金supported by funding from the National Natural Science Foundation of China(32060593 and 32060474)the Yunnan Provincial Science and Technology Department+4 种基金China(202101AT070021 and 202101AS070001)the Yunnan Provincial Department of Education Science Research Fund ProjectChina(2023J0006)the Graduate Innovation Project of Yunnan UniversityChina(KC-22223012 and ZC-22222760)。
文摘Oryza longistaminata is an African wild rice species with valuable agronomic traits and the donor parent of perennial rice.Endophytic bacteria play an important role in host health,adaptive evolution and stress tolerance.However,endophytic bacterial communities in O.longistaminata and their plant growth-promoting(PGP)effects on the perennial rice of O.longistaminata offspring are poorly understood.In this study,the endophytic bacterial diversity,composition and network structures in the root,stem,and leaf tissues of O.longistaminata were characterized using Illumina sequencing of the 16S rRNA gene.The results suggested that O.longistaminata contains a multitude of niches for different endophytic bacteria,among which the root endosphere is more complex and functionally diverse than the stem and leaf endospheres.Tissue-specific biomarkers were identified,including Paludibaculum,Pseudactinotalea and Roseimarinus and others,for roots,Blautia for stems and Lachnospiraceae NK4A136 for leaves.The endophytic bacterial network of O.longistaminata was reassembled for various functions,including degradation/utilization/assimilation,detoxification,generation of precursor metabolites and energy,glycan pathways,macromolecule modification and metabolism.A total of 163 endophytic bacterial strains with PGP traits of potassium release,phosphate solubilization,nitrogen fixation,siderophore activity,indole-3-acetic acid(IAA)production,and 1-aminocyclopropane-1-carboxylate(ACC)deaminase activity were isolated from O.longistaminata.Eleven strains identified as Enterobacter cloacae,Enterobacter ludwigii,Stenotrophomonas maltophilia,Serratia fonticola,and Bacillus velezensis showed stable colonization abilities and PGP effects on perennial rice seedlings.Inoculated plants generally exhibited an enhanced root system and greater photosynthesis,biomass accumulation and nutrient uptake.Interestingly,two strains of E.cloacae have host genotype-dependent effects on perennial rice growth.The results of this study provide insights into the endophytic bacterial ecosystems of O.longistaminata,which can potentially be used as biofertilizers for sustainable perennial rice productivity.
基金supported by the Phytopathology Unit of the Department of Plant Protection(ENA-Meknes,Morocco).
文摘This study investigates the compatibility and efficacy of combining ammonium molybdate(AM)with antagonistic bacteria Bacillus amyloliquefaciens B10W10 and Pseudomonas sp.B11W11 for brown rot control(Monilinia laxa).In vitro experiments reveal variable mycelial growth inhibition rates compared to untreated controls,with B11W11+0.5%AM and B10W10+2%AM displaying the highest inhibition rates after 5 days.After 10 days,the 2%AM+B10W10 combination exhibits the highest inhibition rate.Microscopic observations show structural alterations in mycelium within inhibition zones,marked by vacuolization.The antagonistic bacteria,alone or with different ammonium molybdate concentrations,significantly impact M.laxa spore germination,with the B10W10 cell filtrate+2%ammonium molybdate combination achieving the most substantial inhibition.Conversely,the 0.5%ammonium molybdate treatment has the lowest inhibition rate while the combination of AM and bacteria is giving better results compared to the use of bacteria alone.Fruits treated with various antagonistic bacteria and ammonium molybdate combinations demonstrate a significant reduction in disease severity.The 0.5%AM+B10W10 combination exhibits the lowest severity.FT-IR spectra analysis identifies shifts in fungal biomass functional groups,with reduced lignin-related bands and increased phenols,lipids,polysaccharides,and carbohydrates.This highlights the structural modifications caused by the biological treatments.The study also evaluates the effects on fruit quality parameters.The 2%ammonium molybdate treatment yields the lowest weight loss.TSS levels are affected by salt concentration,while acid content remains consistent across treatments.All treatments influence fruit firmness compared to controls.These findings emphasize the potential of combining ammonium molybdate and antagonistic bacteria for effective brown rot control,highlighting their compatibility and effects on disease severity,fungal biomass,spore germination,and fruit quality.
基金supported by the Fundamental Research Funds of Chinese Academy of Forestry(Nos.CAFYBB2022SY037,CAFYBB2021ZA002 and CAFYBB2022QC002)the Basic Research Foundation of Yunnan Province(Grant No.202201AT070264).
文摘Soil microbial communities are key factors in maintaining ecosystem multifunctionality(EMF).However,the distribution patterns of bacterial diversity and how the different bacterial taxa and their diversity dimensions affect EMF remain largely unknown.Here,we investigated variation in three measures of diversity(alpha diversity,community composition and network complexity)among rare,intermediate,and abundant taxa across a latitudinal gradient spanning five forest plots in Yunnan Province,China and examined their contributions on EMF.We aimed to characterize the diversity distributions of bacterial groups across latitudes and to assess the differences in the mechanisms underlying their contributions to EMF.We found that multifaceted diversity(i.e.,diversity assessed by the three different metrics)of rare,intermediate,and abundant bacteria generally decreased with increasing latitude.More importantly,we found that rare bacterial taxa tended to be more diverse,but they contributed less to EMF than intermediate or abundant bacteria.Among the three dimensions of diversity we assessed,only community composition significantly affected EMF across all locations,while alpha diversity had a negative effect,and network complexity showed no significant impact.Our study further emphasizes the importance of intermediate and abundant bacterial taxa as well as community composition to EMF and provides a theoretical basis for investigating the mechanisms by which belowground microorganisms drive EMF along a latitudinal gradient.
