With different parts of bulb scale as explants, the proliferation method of Guizhou Lilium brownii were studied with 3% sodium hypochlorite and MS medium with different concentrations of hormones. The results show tha...With different parts of bulb scale as explants, the proliferation method of Guizhou Lilium brownii were studied with 3% sodium hypochlorite and MS medium with different concentrations of hormones. The results show that it is feasible to disinfect the bulbs of Lilium brownii with 3% sodium hypoehlorite, moreover, the sodium hypochlorite is very cheap and harmless to researchers, experimental materials and environment. MS ＋ NAA 0.3 mg/L ＋ 6-BA 1.5 mg/L is optimum for the induction of bulbs and, the basal part of Lilium brownie is the optimum explants. After culture for 25 d on the same medium, the tube bulbs could be obtained with the characteristics of high propagation coefficient, strong and new roots. The survival rate is over 90% for transplantation of tube bulbs with diameter between 1-2cm. The method developed in the present study can proliferate abundant Lilium brownii seedling in short time.展开更多
A new natural product (1) together with 26 know compounds were isolated from the Bulbs of Ornithogalum caudatum. Their structures were established on the basis of spectral analyses as n-butyl pyroglutamate (1), nonade...A new natural product (1) together with 26 know compounds were isolated from the Bulbs of Ornithogalum caudatum. Their structures were established on the basis of spectral analyses as n-butyl pyroglutamate (1), nonadecyl alcohol(2), eicosanol(3), behenic acid(4), b-sitosterol(5), stigmasterol(6), glycerol 1-monocerotate(7), pyrocatechol(8), p-ethoxybenzoic acid(9), p-coumarinic acid(10), protocatechuric acid(11), ursolic acid(12), betulinic acid(13), fumaric acid(14), succinic acid(15), uracil(16), xanthine(17), quercetin(18), kaempferol (19), isorham-netin(20), adenosine(21), daucosterol(22), stigmasterol 3-O-b-D-glucopyranoside(23), quercetin 3-O-b-D-glucopyra-noside(24), kaempferol 3-O-b-D-glucopyranoside(25), rutin(26), and kaempferol 3-O-b-rutinoside(27). All of them, except compound 5, were isolated from this plant for the first time.展开更多
Macroautophagy （here autophagy） is a catabolic mechanism responsible for the degradation of bulk cytoplasm, long-lived proteins and organeUes. During autophagy, the cargos are engulfed by double-membrane structures ...Macroautophagy （here autophagy） is a catabolic mechanism responsible for the degradation of bulk cytoplasm, long-lived proteins and organeUes. During autophagy, the cargos are engulfed by double-membrane structures named phagophores, which expand to form the autophagosomes. Subsequently, these autophagosomes fuse with lysosomes, in which the cytoplasmic cargos are degraded. Autophagy is a constitutive pro- cess, which plays an important role in cellular homeostasis. In primary neurons autophagosome formation occurs continuously and preferentially at the distal end of axons. On the other hand, autophagy is increased by different stresses, and its dysregulation or excessive induction may lead to detrimental effects. Many neurological disorders have been associated with alterations in the autophagic pathway and an increase in autophagy during axonal degeneration was described.展开更多
Two new steroidal saponins, named macrostemonoside A and D. were isolated from the bhlbus of Allium macrostemon Bunge (Liliaceae), and their strurtures were established by spectroscopic analysis and chemical evidences.
The ultra-structure of mother and outer daughter scales of Lilium Oriental hybrid Sorbonne were studied using transmission electron microscope to examine the sub-cellular localization of starch and lipid droplets duri...The ultra-structure of mother and outer daughter scales of Lilium Oriental hybrid Sorbonne were studied using transmission electron microscope to examine the sub-cellular localization of starch and lipid droplets during growth and development from shoot emergence to senescence.The contents of starch granules and lipid droplets in the cell of the mother scales decreased significantly from shoot emergence to anthesis,indicating that these scales served as a source for growth and development.After flowering,the number of starch granules and lipid droplets increased dramatically,and finally the cells were filled with the above molecules indicating that the bulb becomes a major sink during bulb enlargement.Ultrastructure observation also showed that symplastic pathway is the main pathway in cells in the exchange and transportation of material during bulb development.The activity of β-amylase,one of the key enzymes catalyzing starch breakdown,showed a similar trend.The enzyme sub-cellular localization via immune-gold electron-microscopy showed that βamylase was predominantly located together with starch granules,while the gold particles were scarcely found in other sub-cellular compartments.The result suggested that this enzyme is compartmented together with its functional substrate supporting its function in catalyzing starch breakdown in living plant cells.展开更多
Onion bulbs were stored at five different temperatures to know the storage behavior of onion bulbs. Indian red onion bulbs were kept without wrapping at ambient conditions (25℃ ± 3℃ and 75% RH) and within polye...Onion bulbs were stored at five different temperatures to know the storage behavior of onion bulbs. Indian red onion bulbs were kept without wrapping at ambient conditions (25℃ ± 3℃ and 75% RH) and within polyethylene pouch at 2.5℃, 6℃, 7℃ and 13℃ for 60 days of storage period. Weight loss, total soluble solids (TSS), number of sprouted and rotten bulbs were measured at an interval of 10 days throughout storage. The greatest decrease (23.25%) in weight was observed throughout storage for onions stored at ambient conditions. TSS was observed to increase in all samples until 40 days of storage and then decreased up to 60 days. Lowest TSS (14.89 ?Bx) was found in onion stored at ambient conditions at the end of storage. Maximum sprouted (67.25%) and rotten (17.78%) onions were observed for onions stored at 13℃, whereas minimum sprouted and rotten onions were found at 2.5℃ at 60 days of storage. Results also recommend lowest temperatures (2.5℃ - 6℃) for prolong storage life of onion with no detrimental effects.展开更多
Lily bulbs are a type of food raw material,but they are less studied as a cosmetic raw material.This study explored the specific components with such inhibitory effects in lily bulbs,to provide a basis for developing ...Lily bulbs are a type of food raw material,but they are less studied as a cosmetic raw material.This study explored the specific components with such inhibitory effects in lily bulbs,to provide a basis for developing functional cosmetic ingredients with skin-soothing properties.High-performance liquid chromatography(HPLC)analysis revealed that the lily bulbs extract(LBE)contains specific components regaloside A,regaloside B,and regaloside C at 2.506 mg/g,1.405 mg/g,and 0.621 mg/g,respectively.Furthermore,network pharmacology analysis showed that these three components acted on key inflammation-related targets such as tumor necrosis factor-alpha(TNF-α),matrix metalloproteinase 2(MMP2),estrogen receptor 1(ESR1),AKT(Protein Kinase B)serine/threonine kinase 1(AKT1),toll-like receptor 4(TLR4),and heat shock protein 90-alpha(HSP90AA1),to regulate signaling pathways,including Pathways in cancer,TNF signaling pathway,Inflammatory mediator regulation of TRP(Transient Receptor Potential)channels,for anti-inflammation.Among them,these components exhibited binding energies of25.02 kJ/mol,22.63 kJ/mol,and22.13 kJ/mol,respectively,with TNF-α.Notably,molecular docking studies demonstrated that,compared to regaloside B and regaloside C,regaloside A bound more closely to key anti-inflammatory targets,including TNF-α,AKT1,TLR4,HSP90AA1,albumin(ALB),and matrix metalloproteinase 9(MMP9).This study pioneered the use of lipopolysaccharide(LPS)to induce HaCaT cells to construct an inflammation model.Additionally,the real-time quantitative polymerase chain reaction(RT-qPCR)analysis of regaloside A at 25μg/mL,50μg/mL,and 100μg/mL revealed a negative correlation between expression levels and sample concentrations,with a 61%reduction in interleukin 6(IL-6)relative mRNA expression and a 60%reduction in inducible nitric oxide synthase(iNOS)relative mRNA expression in the model group at 100μg/mL.These findings indicated that regaloside A was superior to LBE in its anti-inflammatory effect on human skin cells.Combined with findings from molecular docking studies,regaloside A was identified as a functional cosmetic ingredient with skin-soothing properties,offering significant potential for further development.展开更多
Objective To study the benzylphenethylamine alkaloids from the bulbs and flowers of Lycoris radiata.Methods Alkaloids were isolated by various column chromatographic methods and their structures were identified by spe...Objective To study the benzylphenethylamine alkaloids from the bulbs and flowers of Lycoris radiata.Methods Alkaloids were isolated by various column chromatographic methods and their structures were identified by spectral data.Results Fifteen known benzylphenethylamine alkaloids were isolated and identified as lycoramine(1),O-demethyllycoramine(2),N-demethyllycoramine(3),galanthamine(4),lycorine(5),caranine(6),ungminorine(7),narciclasine(8),5-hydroxy-10-O-demethyl-homolycorine(9),hippeastrine(10),ungerine(11),hippeastrine N-oxide(12),O-demethylhaemanthamine(13),haemanthidine(14),and 8-demethoxyhostasine(15).Conclusion Compound 15 is first isolated from the plants in Amaryllidaceae,compounds 3,6,9,and 11 are first reported from the plants in Lycoris Herb.,and compounds 2,7,and 14 are isolated from L.radiata for the first time.The13C-NMR data of compouds 3,7,and 12 are first reported in the present study.Furthermore,the galasine-type alkaloid is isolated from the plants of Lycoris Herb.for the first time.展开更多
The most severe fungal disease is postharvest gray mold,which might cause massive losses in Lanzhou lily bulbs.Some microorganism-produced volatile organic compounds(VOCs)have been proven to have antifungal action and...The most severe fungal disease is postharvest gray mold,which might cause massive losses in Lanzhou lily bulbs.Some microorganism-produced volatile organic compounds(VOCs)have been proven to have antifungal action and can be employed as fruit and vegetable preservation.Our most recent research discovered that diacetyl(2,3-butanedione)produced by Bacillus subtilis CL2 could significantly prevent some pathogens.The goal of this investigation was to see if diacetyl had antifungal properties against Trichoderma lixii F2,one of the most common postharvest rot pathogens of Lanzhou lily bulbs.The results indicated that diacetyl(1.587μL/mL)fumigation treatment significantly inhibited the hyphal growth,modified hyphal morphology,and disrupted the cell membrane integrity.In vivo tests the antifungal mechanisms of diacetyl on T.lixii F2 were performed in Lanzhou lily bulbs.The results indicated that the content of total phenols and flavonoids increased,while malondialdehyde decreased under different treatment by diacetyl.Meanwhile,the activities of peroxidase,polyphenol oxidase,catalase,and superoxide dismutase and phenylalanine ammonia-lyase in Lanzhou lily bulbs were investigated.It was showed that diacetyl could eliminate excessive reactive oxygen species,reduce the damage of plant cells,and improve the resistance of Lanzhou lily bulbs against T.lixii F2.This research reveals that diacetyl could activate antioxidant enzymes and disease-resistant enzymes in Lanzhou lily bulbs to prevent pathogenic fungus from invading and inducing resistance,laying the groundwork for future uses in preserving Lanzhou lily bulbs.展开更多
The effects of brine addition on the dynamic changes in nitrogen transformation,and bacterial community of fermented Allium chinense(A.chinense)bulbs,as well as screening and identification of lactic acid bacteria(LAB...The effects of brine addition on the dynamic changes in nitrogen transformation,and bacterial community of fermented Allium chinense(A.chinense)bulbs,as well as screening and identification of lactic acid bacteria(LAB)with nitrite degradation function were explored in this work.In contrast with natural fermentation(NC)group,supplementation with aged pickle brine(PBa)declined the pH value.In terms of nitrogen metabolism,PBa fermentation could enhance the degradation of nitrite via activating nitrite reductase(NIR).Besides,the ammonium contents showed a small fluctuation without significant difference during fermentation.Furthermore,PBa could alter the microbial diversity of A.chinense bulbs to modulate nitrogen metabolism-related genes including nirA/B/D and narK/G/H.Correlation analysis confirmed that the LAB abundances exhibited significant negative relationships with the levels of nitrite.These results inferred that the microbial pathways of nitrite degradation in A.chinense bulbs might be attributed to denitrification.Accordingly,the present findings suggested that brine fermentation could promote the nitrogen metabolism in A.chinense bulbs via regulating the microbial community and expression of denitrification-related genes,thus shortening the fermentation time compared to the NC group.Moreover,one strain of LAB(L7)was isolated from PBa group by pure culture method,which showed high similarity to Lactiplantibacillus argentoratensis HBUAS570840,and strong nitrite-lowering ability,high salt tolerance,and strong antibacterial activity.Therefore,the addition of brine can be utilized as an effective strategy to modulate the nitrogen metabolism of A.chinense bulbs.展开更多
Adult neurogenesis persists after birth in the subventricular zone, with new neurons migrating to the granule cell layer and glomerular layers of the olfactory bulb, where they integrate into existing circuitry as inh...Adult neurogenesis persists after birth in the subventricular zone, with new neurons migrating to the granule cell layer and glomerular layers of the olfactory bulb, where they integrate into existing circuitry as inhibitory interneurons. The generation of these new neurons in the olfactory bulb supports both structural and functional plasticity, aiding in circuit remodeling triggered by memory and learning processes. However, the presence of these neurons, coupled with the cellular diversity within the olfactory bulb, presents an ongoing challenge in understanding its network organization and function. Moreover,the continuous integration of new neurons in the olfactory bulb plays a pivotal role in regulating olfactory information processing. This adaptive process responds to changes in epithelial composition and contributes to the formation of olfactory memories by modulating cellular connectivity within the olfactory bulb and interacting intricately with higher-order brain regions. The role of adult neurogenesis in olfactory bulb functions remains a topic of debate. Nevertheless, the functionality of the olfactory bulb is intricately linked to the organization of granule cells around mitral and tufted cells. This organizational pattern significantly impacts output, network behavior, and synaptic plasticity, which are crucial for olfactory perception and memory. Additionally, this organization is further shaped by axon terminals originating from cortical and subcortical regions. Despite the crucial role of olfactory bulb in brain functions and behaviors related to olfaction, these complex and highly interconnected processes have not been comprehensively studied as a whole. Therefore, this manuscript aims to discuss our current understanding and explore how neural plasticity and olfactory neurogenesis contribute to enhancing the adaptability of the olfactory system. These mechanisms are thought to support olfactory learning and memory, potentially through increased complexity and restructuring of neural network structures, as well as the addition of new granule granule cells that aid in olfactory adaptation. Additionally, the manuscript underscores the importance of employing precise methodologies to elucidate the specific roles of adult neurogenesis amidst conflicting data and varying experimental paradigms. Understanding these processes is essential for gaining insights into the complexities of olfactory function and behavior.展开更多
Iron is the most abundant transition metal in the brain and is essential for brain development and neuronal function;however,its abnormal accumulation is also implicated in various neurological disorders.The olfactory...Iron is the most abundant transition metal in the brain and is essential for brain development and neuronal function;however,its abnormal accumulation is also implicated in various neurological disorders.The olfactory bulb(OB),an early target in neurodegenerative diseases,acts as a gateway for environmental toxins and contains diverse neuronal populations with distinct roles.This study explored the cell-specific vulnerability to iron in the OB using a mouse model of intranasal administration of ferric ammonium citrate(FAC).Olfactory function was assessed through olfactory discrimination tests,while iron levels in OB tissues,cerebrospinal fluid(CSF),and serum were quantified using inductively coupled plasma mass spectrometry(ICP-MS),immunohistochemical staining,and iron assays.Transcriptomic changes and immune responses were assessed using RNA sequencing and immune cell infiltration analysis.Results showed that intranasal FAC administration impaired olfactory function,accompanied by iron deposition in the olfactory mucosa and OB,as well as damage to olfactory sensory neurons.Notably,these effects occurred without elevations in CSF or serum iron levels.OB iron accumulation activated multiple immune cells,including microglia and astrocytes,but did not trigger ferroptosis.Spatial transcriptomic sequencing of healthy adult mouse OBs revealed significant cellular heterogeneity,with an abundance of neuroglia and neurons.Among neurons,GABAergic neurons were the most prevalent,followed by glutamatergic and dopaminergic neurons,while cholinergic and serotonergic neurons were sparsely distributed.Under iron-stressed conditions,oligodendrocytes,dopaminergic neurons,and glutamatergic neurons exhibited significant damage,while GABAergic neurons remained unaffected.These findings highlight the selective vulnerability of neuronal and glial populations to iron-induced stress,offering novel insights into the loss of specific cell types in the OB during iron dysregulation.展开更多
Objective:This study assessed heat exposure,physiological responses,and the risk of chronic kidney disease among outdoor agricultural workers in Pak Chong,Thailand,and developed a predictive model for Wet Bulb Globe T...Objective:This study assessed heat exposure,physiological responses,and the risk of chronic kidney disease among outdoor agricultural workers in Pak Chong,Thailand,and developed a predictive model for Wet Bulb Globe Temperature(WBGT).Methods:A cross-sectional study was conducted among 170 agricultural workers.Field WBGT was measured at 33 locations in April and July and corrected for Clothing Adjustment Factor to determine effective WBGT.Core temperature,skin temperature,heart rate,blood pressure,and body weight were monitored before,during,and after work shifts.Urine dipstick and microalbumin tests were performed to evaluate dehydration and kidney disorder.Workloads were classified according to American Conference of Governmental Industrial Hygienists(ACGIH)metabolic rate categories.Meteorological data were used to train machine learning models—including linear regression,decision tree,random forest,and extreme gradient boosting—to predict WBGT values.Results:Effective WBGT at fruit gardens and field crops sites frequently exceeded ACGIH limits for moderateto-heavy work.Workers’systolic/diastolic blood pressure and heart rate increased during work(transient cardiovascular strain)while body temperature remained stable.Post-shift,34.7%showed moderate dehydration;microalbumin screening was positive in 31/170(18.2%)suggesting possible early renal involvement but not chronic kidney disease diagnosis.Among the predictive models,extreme gradient boosting achieved the best performance(root mean square error=0.798℃,mean absolute error=0.576℃,R^(2)=0.81).The most influential predictors were air temperature,sunshine duration,and site-specific factors.Conclusions:Agricultural workers in Pak Chong are frequently exposed to heat stress that exceeds ACGIH standards,predisposing them to dehydration and kidney impairment.The findings support evidence-based preventive strategies such as regulated work–rest cycles,hydration management,and medical surveillance.Moreover,the validated WBGT prediction tool offers a practical approach for real-time monitoring and proactive climate adaptation to protect outdoor workers in heat-prone environments.展开更多
Objective] The aim of this study was to investigate the electrophysiological characteristics of spontaneous electrical activities of olfactory bulb in Bufo gargarizans during postembryonic development. [Method] Microe...Objective] The aim of this study was to investigate the electrophysiological characteristics of spontaneous electrical activities of olfactory bulb in Bufo gargarizans during postembryonic development. [Method] Microelectrode technique was used to record extracellular spontaneous electrical activities of olfactory bulb in B. gar-garizans. [Result] Along with the development of olfactory bulb, the discharge types increased gradual y. Single spontaneous firing enhanced gradual y and it reached the maximum in the metamorphosis phase. The intensity of consecutive single sponta-neous firing changed slightly at the middle of post-embryonic development, but it was stil higher than in the early phase and the adult phase. Consecutive burst spontaneous firing frequency went down over time. [Conclusion] Along with the de-velopment of olfactory bulb, the neuronal excitability increases step by step and the neuronal electrical activities diversify gradualy.展开更多
The purpose of this article is to detect sex and age difference in the structure of the olfactory bulb in dogs by histological methods. The thickness of the olfactory bulbs layers and its main cells were analyzed comp...The purpose of this article is to detect sex and age difference in the structure of the olfactory bulb in dogs by histological methods. The thickness of the olfactory bulbs layers and its main cells were analyzed comparatively with the methods of HE-staining and statistics, through which we studied the development course of dogs＇ olfactory bulb and the structural differences which affect the olfaction in both males and females. The results showed that between both male and female juveniles and adult males and females, the difference in thickness of each layer is not significant. But the difference in quantity of mitral cells between adult males and females was significant. Meanwhile, the structure of every layer in juvenile dogs was apparent while the volume and the weight of adult dogs＇ olfactory bulb and each layer＇s width increased significantly. On the other hand, the density of each layer＇s cells decreased apparently. Our results demonstrated that the olfactory bulb developed with age, and the apparent differences in morphology and quantity of mitral cells between males and females may be one of the reasons leading to the sexual variations of olfactory sensitivity.展开更多
[Objective] The purpose of this study is to investigate the effects of differ- ent phytohormones on the adventitious bud differentiation of oriental lily. [Method] The bulb scales of the test-tube plantlets of Tiber, ...[Objective] The purpose of this study is to investigate the effects of differ- ent phytohormones on the adventitious bud differentiation of oriental lily. [Method] The bulb scales of the test-tube plantlets of Tiber, Rodina and Constanta were cul- tured in media supplemented with different cytokinin and auxin at different concen- tration, and then the adventitious buds in each treatment were calculated. [Result] Cytokinins had different influence on the adventitious bud differentiation of the three oriental lily cultivars. Among them, 6-BA had the best effect to induce the adventi- tious bud differentiation from bulb scales of Tiber and Rodina, but there was some difference in the optimal concentration. KT had the best effect to induce the adven- titious bud differentiation of Constanta. The auxins had little influence on the quality of the adventitious bud of the three oriental lily cultivars, but caused some difference in differentiation coefficients. [Conclusion] The most suitable media for the adventi- tious bud differentiation from bulb scales in vitro of Tiber, Rodina and Constanta were MS＋0.2 mg/L 6-BA＋0.2 mg/L 2,4-D, MS＋I.0 mg/L 6-BA＋0.2 mg/L IAA, MS＋ 1.0 mg/L KT＋0.5 mg/L 2,4-D, respectively.展开更多
The dynamics of the growth of roots and leaves of the Allium cepa L. after the mechanical removal of a part of the root system or the bulb was studied. It was shown that, after surgical interference, the potency for o...The dynamics of the growth of roots and leaves of the Allium cepa L. after the mechanical removal of a part of the root system or the bulb was studied. It was shown that, after surgical interference, the potency for organogenesis is retained, although it is weakened.展开更多
A pot experiment has been conducted in Faculty of Agricultural and Forestry, Lath House of Horticultural Department, School of Plant Production, Duhok University during the growing season 2010-2011. The study consiste...A pot experiment has been conducted in Faculty of Agricultural and Forestry, Lath House of Horticultural Department, School of Plant Production, Duhok University during the growing season 2010-2011. The study consisted of testing the effects of four different growth media （Clay, Loam, Clay ＋ sheep manure （1：1） and Loam ＋ sheep manure （1：1） as volumetric rates on vegetative and flowering growth characters of five different cultivars of Hyacinths plant （Blue Giant, City of Haarlem, Jon Bos, Delf Blue and Fondante）. The experiment was laid out in randomized complete block design （RCBD） of two factors in three replicates. Media mostly affected the parameters studied during the experiment. Early emergence （22.53 days） and flowering （73.13 days） was recorded in medium consisting of loam and sheep manure, while maximum plant height （19.75 cm）, leaves number （6.13）, leaf area （31.47 cm2） were recorded in clay medium. Maximum number of florets plant-l （31.93） and spike length （7.95 cm） were recorded in medium consist of clay and sheep manure. Maximum length of spike stem （9.61 cm） and total chlorophyll percentage （53.45%） were recorded for loam medium. Among the cultivars, some parameters showed significant variation. Early emergence （20.42 days）, maximum plant height （20.35 cm）, leaf area per plant （33.01 cm2） and length of spike stem （15.10 cm） were given by cv. Fondante. Maximum number of florets plantl （35.58） and spike lengths （8.63 cm） were recorded by cv. Blue Giant. High percentage of total chlorophyll （58.15%） was recorded in cv. Jon Bos..展开更多
基金Supported by the Nomarch Funds for Excellent Science and Technology Teachers of Guizhou Province(S2004-17)the Special Foundation for Im-proving Scientific Research Condition of Guizhou Province(Q2005-4)the Doctor Startup of Guiyang Medical College(C2005-6)~~
文摘With different parts of bulb scale as explants, the proliferation method of Guizhou Lilium brownii were studied with 3% sodium hypochlorite and MS medium with different concentrations of hormones. The results show that it is feasible to disinfect the bulbs of Lilium brownii with 3% sodium hypoehlorite, moreover, the sodium hypochlorite is very cheap and harmless to researchers, experimental materials and environment. MS ＋ NAA 0.3 mg/L ＋ 6-BA 1.5 mg/L is optimum for the induction of bulbs and, the basal part of Lilium brownie is the optimum explants. After culture for 25 d on the same medium, the tube bulbs could be obtained with the characteristics of high propagation coefficient, strong and new roots. The survival rate is over 90% for transplantation of tube bulbs with diameter between 1-2cm. The method developed in the present study can proliferate abundant Lilium brownii seedling in short time.
基金This work was supported by the Ministry of Science and Technology of China (96-901-05-266).
文摘A new natural product (1) together with 26 know compounds were isolated from the Bulbs of Ornithogalum caudatum. Their structures were established on the basis of spectral analyses as n-butyl pyroglutamate (1), nonadecyl alcohol(2), eicosanol(3), behenic acid(4), b-sitosterol(5), stigmasterol(6), glycerol 1-monocerotate(7), pyrocatechol(8), p-ethoxybenzoic acid(9), p-coumarinic acid(10), protocatechuric acid(11), ursolic acid(12), betulinic acid(13), fumaric acid(14), succinic acid(15), uracil(16), xanthine(17), quercetin(18), kaempferol (19), isorham-netin(20), adenosine(21), daucosterol(22), stigmasterol 3-O-b-D-glucopyranoside(23), quercetin 3-O-b-D-glucopyra-noside(24), kaempferol 3-O-b-D-glucopyranoside(25), rutin(26), and kaempferol 3-O-b-rutinoside(27). All of them, except compound 5, were isolated from this plant for the first time.
基金the National Council for Scientific and Technological Development(CNPq)the International Foundation for Research in Paraplegia(IRP-P 112)+1 种基金the Deutsche Forschungsgemeinschaft(DFG-LI 1308/3-1)the Else Kr?ner-Fresenius-Stiftung
文摘Macroautophagy （here autophagy） is a catabolic mechanism responsible for the degradation of bulk cytoplasm, long-lived proteins and organeUes. During autophagy, the cargos are engulfed by double-membrane structures named phagophores, which expand to form the autophagosomes. Subsequently, these autophagosomes fuse with lysosomes, in which the cytoplasmic cargos are degraded. Autophagy is a constitutive pro- cess, which plays an important role in cellular homeostasis. In primary neurons autophagosome formation occurs continuously and preferentially at the distal end of axons. On the other hand, autophagy is increased by different stresses, and its dysregulation or excessive induction may lead to detrimental effects. Many neurological disorders have been associated with alterations in the autophagic pathway and an increase in autophagy during axonal degeneration was described.
基金The project supported by Lhe National Natural Science Foundation of China.
文摘Two new steroidal saponins, named macrostemonoside A and D. were isolated from the bhlbus of Allium macrostemon Bunge (Liliaceae), and their strurtures were established by spectroscopic analysis and chemical evidences.
基金supported by the National Natural Science Foundation of China(31071815)the National HighTech R&D Program of China(2011AA100208)
文摘The ultra-structure of mother and outer daughter scales of Lilium Oriental hybrid Sorbonne were studied using transmission electron microscope to examine the sub-cellular localization of starch and lipid droplets during growth and development from shoot emergence to senescence.The contents of starch granules and lipid droplets in the cell of the mother scales decreased significantly from shoot emergence to anthesis,indicating that these scales served as a source for growth and development.After flowering,the number of starch granules and lipid droplets increased dramatically,and finally the cells were filled with the above molecules indicating that the bulb becomes a major sink during bulb enlargement.Ultrastructure observation also showed that symplastic pathway is the main pathway in cells in the exchange and transportation of material during bulb development.The activity of β-amylase,one of the key enzymes catalyzing starch breakdown,showed a similar trend.The enzyme sub-cellular localization via immune-gold electron-microscopy showed that βamylase was predominantly located together with starch granules,while the gold particles were scarcely found in other sub-cellular compartments.The result suggested that this enzyme is compartmented together with its functional substrate supporting its function in catalyzing starch breakdown in living plant cells.
文摘Onion bulbs were stored at five different temperatures to know the storage behavior of onion bulbs. Indian red onion bulbs were kept without wrapping at ambient conditions (25℃ ± 3℃ and 75% RH) and within polyethylene pouch at 2.5℃, 6℃, 7℃ and 13℃ for 60 days of storage period. Weight loss, total soluble solids (TSS), number of sprouted and rotten bulbs were measured at an interval of 10 days throughout storage. The greatest decrease (23.25%) in weight was observed throughout storage for onions stored at ambient conditions. TSS was observed to increase in all samples until 40 days of storage and then decreased up to 60 days. Lowest TSS (14.89 ?Bx) was found in onion stored at ambient conditions at the end of storage. Maximum sprouted (67.25%) and rotten (17.78%) onions were observed for onions stored at 13℃, whereas minimum sprouted and rotten onions were found at 2.5℃ at 60 days of storage. Results also recommend lowest temperatures (2.5℃ - 6℃) for prolong storage life of onion with no detrimental effects.
基金funded by Natural Science Foundation of Hunan Province Fund(Grant numbers:2021JJ80054).
文摘Lily bulbs are a type of food raw material,but they are less studied as a cosmetic raw material.This study explored the specific components with such inhibitory effects in lily bulbs,to provide a basis for developing functional cosmetic ingredients with skin-soothing properties.High-performance liquid chromatography(HPLC)analysis revealed that the lily bulbs extract(LBE)contains specific components regaloside A,regaloside B,and regaloside C at 2.506 mg/g,1.405 mg/g,and 0.621 mg/g,respectively.Furthermore,network pharmacology analysis showed that these three components acted on key inflammation-related targets such as tumor necrosis factor-alpha(TNF-α),matrix metalloproteinase 2(MMP2),estrogen receptor 1(ESR1),AKT(Protein Kinase B)serine/threonine kinase 1(AKT1),toll-like receptor 4(TLR4),and heat shock protein 90-alpha(HSP90AA1),to regulate signaling pathways,including Pathways in cancer,TNF signaling pathway,Inflammatory mediator regulation of TRP(Transient Receptor Potential)channels,for anti-inflammation.Among them,these components exhibited binding energies of25.02 kJ/mol,22.63 kJ/mol,and22.13 kJ/mol,respectively,with TNF-α.Notably,molecular docking studies demonstrated that,compared to regaloside B and regaloside C,regaloside A bound more closely to key anti-inflammatory targets,including TNF-α,AKT1,TLR4,HSP90AA1,albumin(ALB),and matrix metalloproteinase 9(MMP9).This study pioneered the use of lipopolysaccharide(LPS)to induce HaCaT cells to construct an inflammation model.Additionally,the real-time quantitative polymerase chain reaction(RT-qPCR)analysis of regaloside A at 25μg/mL,50μg/mL,and 100μg/mL revealed a negative correlation between expression levels and sample concentrations,with a 61%reduction in interleukin 6(IL-6)relative mRNA expression and a 60%reduction in inducible nitric oxide synthase(iNOS)relative mRNA expression in the model group at 100μg/mL.These findings indicated that regaloside A was superior to LBE in its anti-inflammatory effect on human skin cells.Combined with findings from molecular docking studies,regaloside A was identified as a functional cosmetic ingredient with skin-soothing properties,offering significant potential for further development.
基金National Natural Science Foundation of China(20972166&31070288)Ministry of Education of China through its 985 and 111 projects(MUC 98503-001006&B08044)
文摘Objective To study the benzylphenethylamine alkaloids from the bulbs and flowers of Lycoris radiata.Methods Alkaloids were isolated by various column chromatographic methods and their structures were identified by spectral data.Results Fifteen known benzylphenethylamine alkaloids were isolated and identified as lycoramine(1),O-demethyllycoramine(2),N-demethyllycoramine(3),galanthamine(4),lycorine(5),caranine(6),ungminorine(7),narciclasine(8),5-hydroxy-10-O-demethyl-homolycorine(9),hippeastrine(10),ungerine(11),hippeastrine N-oxide(12),O-demethylhaemanthamine(13),haemanthidine(14),and 8-demethoxyhostasine(15).Conclusion Compound 15 is first isolated from the plants in Amaryllidaceae,compounds 3,6,9,and 11 are first reported from the plants in Lycoris Herb.,and compounds 2,7,and 14 are isolated from L.radiata for the first time.The13C-NMR data of compouds 3,7,and 12 are first reported in the present study.Furthermore,the galasine-type alkaloid is isolated from the plants of Lycoris Herb.for the first time.
基金This work was supported by the Lanzhou Science and Technology Plan Project[grant number:2018-1-104]the Special Fund Project for Guiding Science and Technology Innovation and Development in Gansu Province[grant number:2019ZX-05]the Gansu Province Colleges and Universities Industrial Support Plan Project[grant number:2020C-21].
文摘The most severe fungal disease is postharvest gray mold,which might cause massive losses in Lanzhou lily bulbs.Some microorganism-produced volatile organic compounds(VOCs)have been proven to have antifungal action and can be employed as fruit and vegetable preservation.Our most recent research discovered that diacetyl(2,3-butanedione)produced by Bacillus subtilis CL2 could significantly prevent some pathogens.The goal of this investigation was to see if diacetyl had antifungal properties against Trichoderma lixii F2,one of the most common postharvest rot pathogens of Lanzhou lily bulbs.The results indicated that diacetyl(1.587μL/mL)fumigation treatment significantly inhibited the hyphal growth,modified hyphal morphology,and disrupted the cell membrane integrity.In vivo tests the antifungal mechanisms of diacetyl on T.lixii F2 were performed in Lanzhou lily bulbs.The results indicated that the content of total phenols and flavonoids increased,while malondialdehyde decreased under different treatment by diacetyl.Meanwhile,the activities of peroxidase,polyphenol oxidase,catalase,and superoxide dismutase and phenylalanine ammonia-lyase in Lanzhou lily bulbs were investigated.It was showed that diacetyl could eliminate excessive reactive oxygen species,reduce the damage of plant cells,and improve the resistance of Lanzhou lily bulbs against T.lixii F2.This research reveals that diacetyl could activate antioxidant enzymes and disease-resistant enzymes in Lanzhou lily bulbs to prevent pathogenic fungus from invading and inducing resistance,laying the groundwork for future uses in preserving Lanzhou lily bulbs.
基金funded by Hunan Provincial Science Fund for Distinguished Young Scholars,China(2021JJ10007)the Research and Development Program in Key Areas of Hunan Province,China(2021NK2015,2019SK2121)Hunan Scientific Talents Promotion Program supported by Hunan Association for Science and Technology,China(2019TJ-Q01).
文摘The effects of brine addition on the dynamic changes in nitrogen transformation,and bacterial community of fermented Allium chinense(A.chinense)bulbs,as well as screening and identification of lactic acid bacteria(LAB)with nitrite degradation function were explored in this work.In contrast with natural fermentation(NC)group,supplementation with aged pickle brine(PBa)declined the pH value.In terms of nitrogen metabolism,PBa fermentation could enhance the degradation of nitrite via activating nitrite reductase(NIR).Besides,the ammonium contents showed a small fluctuation without significant difference during fermentation.Furthermore,PBa could alter the microbial diversity of A.chinense bulbs to modulate nitrogen metabolism-related genes including nirA/B/D and narK/G/H.Correlation analysis confirmed that the LAB abundances exhibited significant negative relationships with the levels of nitrite.These results inferred that the microbial pathways of nitrite degradation in A.chinense bulbs might be attributed to denitrification.Accordingly,the present findings suggested that brine fermentation could promote the nitrogen metabolism in A.chinense bulbs via regulating the microbial community and expression of denitrification-related genes,thus shortening the fermentation time compared to the NC group.Moreover,one strain of LAB(L7)was isolated from PBa group by pure culture method,which showed high similarity to Lactiplantibacillus argentoratensis HBUAS570840,and strong nitrite-lowering ability,high salt tolerance,and strong antibacterial activity.Therefore,the addition of brine can be utilized as an effective strategy to modulate the nitrogen metabolism of A.chinense bulbs.
文摘Adult neurogenesis persists after birth in the subventricular zone, with new neurons migrating to the granule cell layer and glomerular layers of the olfactory bulb, where they integrate into existing circuitry as inhibitory interneurons. The generation of these new neurons in the olfactory bulb supports both structural and functional plasticity, aiding in circuit remodeling triggered by memory and learning processes. However, the presence of these neurons, coupled with the cellular diversity within the olfactory bulb, presents an ongoing challenge in understanding its network organization and function. Moreover,the continuous integration of new neurons in the olfactory bulb plays a pivotal role in regulating olfactory information processing. This adaptive process responds to changes in epithelial composition and contributes to the formation of olfactory memories by modulating cellular connectivity within the olfactory bulb and interacting intricately with higher-order brain regions. The role of adult neurogenesis in olfactory bulb functions remains a topic of debate. Nevertheless, the functionality of the olfactory bulb is intricately linked to the organization of granule cells around mitral and tufted cells. This organizational pattern significantly impacts output, network behavior, and synaptic plasticity, which are crucial for olfactory perception and memory. Additionally, this organization is further shaped by axon terminals originating from cortical and subcortical regions. Despite the crucial role of olfactory bulb in brain functions and behaviors related to olfaction, these complex and highly interconnected processes have not been comprehensively studied as a whole. Therefore, this manuscript aims to discuss our current understanding and explore how neural plasticity and olfactory neurogenesis contribute to enhancing the adaptability of the olfactory system. These mechanisms are thought to support olfactory learning and memory, potentially through increased complexity and restructuring of neural network structures, as well as the addition of new granule granule cells that aid in olfactory adaptation. Additionally, the manuscript underscores the importance of employing precise methodologies to elucidate the specific roles of adult neurogenesis amidst conflicting data and varying experimental paradigms. Understanding these processes is essential for gaining insights into the complexities of olfactory function and behavior.
基金supported by the National Natural Science Foundation of China (32471188,32170984,82301787)。
文摘Iron is the most abundant transition metal in the brain and is essential for brain development and neuronal function;however,its abnormal accumulation is also implicated in various neurological disorders.The olfactory bulb(OB),an early target in neurodegenerative diseases,acts as a gateway for environmental toxins and contains diverse neuronal populations with distinct roles.This study explored the cell-specific vulnerability to iron in the OB using a mouse model of intranasal administration of ferric ammonium citrate(FAC).Olfactory function was assessed through olfactory discrimination tests,while iron levels in OB tissues,cerebrospinal fluid(CSF),and serum were quantified using inductively coupled plasma mass spectrometry(ICP-MS),immunohistochemical staining,and iron assays.Transcriptomic changes and immune responses were assessed using RNA sequencing and immune cell infiltration analysis.Results showed that intranasal FAC administration impaired olfactory function,accompanied by iron deposition in the olfactory mucosa and OB,as well as damage to olfactory sensory neurons.Notably,these effects occurred without elevations in CSF or serum iron levels.OB iron accumulation activated multiple immune cells,including microglia and astrocytes,but did not trigger ferroptosis.Spatial transcriptomic sequencing of healthy adult mouse OBs revealed significant cellular heterogeneity,with an abundance of neuroglia and neurons.Among neurons,GABAergic neurons were the most prevalent,followed by glutamatergic and dopaminergic neurons,while cholinergic and serotonergic neurons were sparsely distributed.Under iron-stressed conditions,oligodendrocytes,dopaminergic neurons,and glutamatergic neurons exhibited significant damage,while GABAergic neurons remained unaffected.These findings highlight the selective vulnerability of neuronal and glial populations to iron-induced stress,offering novel insights into the loss of specific cell types in the OB during iron dysregulation.
基金supported by Suranaree University of Technology,the National Science,Research and Innovation Fundthe Program Management Unit for Human Resources&Institutional Development,Research and Innovation,Fiscal Year 2024.
文摘Objective:This study assessed heat exposure,physiological responses,and the risk of chronic kidney disease among outdoor agricultural workers in Pak Chong,Thailand,and developed a predictive model for Wet Bulb Globe Temperature(WBGT).Methods:A cross-sectional study was conducted among 170 agricultural workers.Field WBGT was measured at 33 locations in April and July and corrected for Clothing Adjustment Factor to determine effective WBGT.Core temperature,skin temperature,heart rate,blood pressure,and body weight were monitored before,during,and after work shifts.Urine dipstick and microalbumin tests were performed to evaluate dehydration and kidney disorder.Workloads were classified according to American Conference of Governmental Industrial Hygienists(ACGIH)metabolic rate categories.Meteorological data were used to train machine learning models—including linear regression,decision tree,random forest,and extreme gradient boosting—to predict WBGT values.Results:Effective WBGT at fruit gardens and field crops sites frequently exceeded ACGIH limits for moderateto-heavy work.Workers’systolic/diastolic blood pressure and heart rate increased during work(transient cardiovascular strain)while body temperature remained stable.Post-shift,34.7%showed moderate dehydration;microalbumin screening was positive in 31/170(18.2%)suggesting possible early renal involvement but not chronic kidney disease diagnosis.Among the predictive models,extreme gradient boosting achieved the best performance(root mean square error=0.798℃,mean absolute error=0.576℃,R^(2)=0.81).The most influential predictors were air temperature,sunshine duration,and site-specific factors.Conclusions:Agricultural workers in Pak Chong are frequently exposed to heat stress that exceeds ACGIH standards,predisposing them to dehydration and kidney impairment.The findings support evidence-based preventive strategies such as regulated work–rest cycles,hydration management,and medical surveillance.Moreover,the validated WBGT prediction tool offers a practical approach for real-time monitoring and proactive climate adaptation to protect outdoor workers in heat-prone environments.
基金Supported by grants from the Liaoning Provincial Department of Education(2011196)the Experimental Center Director Fund of Shenyang Normal University(sy201003)~~
文摘Objective] The aim of this study was to investigate the electrophysiological characteristics of spontaneous electrical activities of olfactory bulb in Bufo gargarizans during postembryonic development. [Method] Microelectrode technique was used to record extracellular spontaneous electrical activities of olfactory bulb in B. gar-garizans. [Result] Along with the development of olfactory bulb, the discharge types increased gradual y. Single spontaneous firing enhanced gradual y and it reached the maximum in the metamorphosis phase. The intensity of consecutive single sponta-neous firing changed slightly at the middle of post-embryonic development, but it was stil higher than in the early phase and the adult phase. Consecutive burst spontaneous firing frequency went down over time. [Conclusion] Along with the de-velopment of olfactory bulb, the neuronal excitability increases step by step and the neuronal electrical activities diversify gradualy.
基金National Natural Science Foundation of China (30370218)Shan-dong Science Fund for Distinguished Young Scholars(2005BS02005)Science Foundation for Excellent Scholars of Ministry of Education of China (NCET-07-0507)
文摘The purpose of this article is to detect sex and age difference in the structure of the olfactory bulb in dogs by histological methods. The thickness of the olfactory bulbs layers and its main cells were analyzed comparatively with the methods of HE-staining and statistics, through which we studied the development course of dogs＇ olfactory bulb and the structural differences which affect the olfaction in both males and females. The results showed that between both male and female juveniles and adult males and females, the difference in thickness of each layer is not significant. But the difference in quantity of mitral cells between adult males and females was significant. Meanwhile, the structure of every layer in juvenile dogs was apparent while the volume and the weight of adult dogs＇ olfactory bulb and each layer＇s width increased significantly. On the other hand, the density of each layer＇s cells decreased apparently. Our results demonstrated that the olfactory bulb developed with age, and the apparent differences in morphology and quantity of mitral cells between males and females may be one of the reasons leading to the sexual variations of olfactory sensitivity.
基金Supported by Special Fund for the Dominant Subjects Development of the Higher Education Institutions of Jiangsu ProvinceScience and Technology Innovation Fund of Nanjing Forestry University(X09-120-4)~~
文摘[Objective] The purpose of this study is to investigate the effects of differ- ent phytohormones on the adventitious bud differentiation of oriental lily. [Method] The bulb scales of the test-tube plantlets of Tiber, Rodina and Constanta were cul- tured in media supplemented with different cytokinin and auxin at different concen- tration, and then the adventitious buds in each treatment were calculated. [Result] Cytokinins had different influence on the adventitious bud differentiation of the three oriental lily cultivars. Among them, 6-BA had the best effect to induce the adventi- tious bud differentiation from bulb scales of Tiber and Rodina, but there was some difference in the optimal concentration. KT had the best effect to induce the adven- titious bud differentiation of Constanta. The auxins had little influence on the quality of the adventitious bud of the three oriental lily cultivars, but caused some difference in differentiation coefficients. [Conclusion] The most suitable media for the adventi- tious bud differentiation from bulb scales in vitro of Tiber, Rodina and Constanta were MS＋0.2 mg/L 6-BA＋0.2 mg/L 2,4-D, MS＋I.0 mg/L 6-BA＋0.2 mg/L IAA, MS＋ 1.0 mg/L KT＋0.5 mg/L 2,4-D, respectively.
文摘The dynamics of the growth of roots and leaves of the Allium cepa L. after the mechanical removal of a part of the root system or the bulb was studied. It was shown that, after surgical interference, the potency for organogenesis is retained, although it is weakened.
文摘A pot experiment has been conducted in Faculty of Agricultural and Forestry, Lath House of Horticultural Department, School of Plant Production, Duhok University during the growing season 2010-2011. The study consisted of testing the effects of four different growth media （Clay, Loam, Clay ＋ sheep manure （1：1） and Loam ＋ sheep manure （1：1） as volumetric rates on vegetative and flowering growth characters of five different cultivars of Hyacinths plant （Blue Giant, City of Haarlem, Jon Bos, Delf Blue and Fondante）. The experiment was laid out in randomized complete block design （RCBD） of two factors in three replicates. Media mostly affected the parameters studied during the experiment. Early emergence （22.53 days） and flowering （73.13 days） was recorded in medium consisting of loam and sheep manure, while maximum plant height （19.75 cm）, leaves number （6.13）, leaf area （31.47 cm2） were recorded in clay medium. Maximum number of florets plant-l （31.93） and spike length （7.95 cm） were recorded in medium consist of clay and sheep manure. Maximum length of spike stem （9.61 cm） and total chlorophyll percentage （53.45%） were recorded for loam medium. Among the cultivars, some parameters showed significant variation. Early emergence （20.42 days）, maximum plant height （20.35 cm）, leaf area per plant （33.01 cm2） and length of spike stem （15.10 cm） were given by cv. Fondante. Maximum number of florets plantl （35.58） and spike lengths （8.63 cm） were recorded by cv. Blue Giant. High percentage of total chlorophyll （58.15%） was recorded in cv. Jon Bos..
