The polysaccharides in yam bulbils were extracted, and their antioxidant activity and hypoglycemic effect in diabetic mice were discussed. The results showed that the antioxidant activity of polysaccharides in yam bul...The polysaccharides in yam bulbils were extracted, and their antioxidant activity and hypoglycemic effect in diabetic mice were discussed. The results showed that the antioxidant activity of polysaccharides in yam bulbils was signifi- cantly enhanced with the increase of concentration; they showed a strong scaveng- ing ability against DPPH. and .OH, and the scavenging ability was dose dependent to some extent; the scavenging rates reached 91.15% and 89.06% respectively when the dose reached 4.0 mg/ml; the polysaccharides in yam bulbils significantly educed the blood glucose in model rice, and the hypoglycemic effect of large-dose polysaccharides was more obvious. The polysaccharides in yam bulbils has good antioxidant activity and hypoglycemic effect, which provides a new source for devel- opment of safe and natural food antioxidants and blood sugar-lowering agents.展开更多
Bistorta vivipara is a facultative reproductive plant capable of asexual reproduction through underground rhizomes and bulbils,as well as sexual reproduction via seeds.The phenomenon of vegetative organ vivipary is a ...Bistorta vivipara is a facultative reproductive plant capable of asexual reproduction through underground rhizomes and bulbils,as well as sexual reproduction via seeds.The phenomenon of vegetative organ vivipary is a complex biological process regulated by a network of genes.However,the developmental mechanism regulating bulbil vivipary in B.vivipara remains largely unexplored.This study investigated different developmental stages of B.vivipara using RNA sequencing and transcriptome analysis.Approximately 438 million high-quality reads were generated,with over 61.65%of the data mapped to the de novo transcriptome sequence.A total of 154,813 reads were matched in at least one public database,and 49,731 genes were differentially expressed across developmental stages.Functional analysis revealed significant enrichment of these genes in phenylpropanoid biosynthesis,plant hormone signal transduction,protein processing,starch and sucrose metabolism,and plant-pathogen interaction.Ninety-four genes involved in phytohormones,plant pigments,enzymes,and transcription factors were identified as potential candidates for inducing vegetative organ vivipary.These differentially expressed genes(DEGs),detected through comparative transcriptome analysis,may serve as candidate genes for bulbil vivipary in B.vivipara,establishing a foundation for future studies on the molecular mechanisms underlying vegetative organ vivipary.展开更多
Aims Many polyploids are geographically separated from their lower-ploidy progenitors at a variety of spatial scales.Diverse adaptive and non-adaptive mechanisms have been suggested to contribute to these spatial patt...Aims Many polyploids are geographically separated from their lower-ploidy progenitors at a variety of spatial scales.Diverse adaptive and non-adaptive mechanisms have been suggested to contribute to these spatial patterns.A long-standing hypothesis holds that poly-ploids are established and persist due to specialization to differ-ent ecological conditions compared to their progenitors.However,experimental approaches testing this hypothesis remain rare.Empirical evidence for niche differences among ploidy levels has been found previously for the European polyploid geophyte Allium oleraceum.Here,we conducted a 5-year reciprocal transplant experiment with tetraploid,pentaploid and hexaploid A.oleraceum to test for adaptation at the ploidy and population levels.Methods In 2006,we sowed 2160 aerial bulbils among six sites(two per cytotype,central and western Moravia,the Czech Republic)and monitored their emergence in 2007,their survival until 2011 and their performance in 2011.Important Findings The emergence of bulbils was high at all target sites,and no evi-dence for niche differentiation among cytotypes was recorded in the first year.Survival in the following years was relatively high,but significantly better survival for plants of home ploidy than foreign ploidy was recorded.No plant reached the reproductive stage during our experiment.Adopting a strict definition of local adaptation,local plants only rarely performed better than foreign plants at both sites in pairwise comparisons within a cytotype.Together,our results sug-gest weak niche differentiation among cytotypes and provide limited support for the existence of local adaptation within cytotypes.Thus,all cytotypes are able to establish in habitats typically populated by other cytotypes via aerial bulbil spread,but will likely die out gradually in non-native habitats.This ability may explain the occur-rence of some multiple-cytotype populations.The complex spatial pattern of A.oleraceum cytotypes is therefore the result of several interacting factors,including niche differentiation among cytotypes,the different evolutionary ages of cytotypes,the existence of gene flow between and within cytotypes,the dominance of clonality and stochastic events linked to anthropogenic dispersal and disturbance.展开更多
基金Supported by Key Project of Huainan Normal College(2011LK76zd)Youth Fund of Huainan Normal College(2010LK13)~~
文摘The polysaccharides in yam bulbils were extracted, and their antioxidant activity and hypoglycemic effect in diabetic mice were discussed. The results showed that the antioxidant activity of polysaccharides in yam bulbils was signifi- cantly enhanced with the increase of concentration; they showed a strong scaveng- ing ability against DPPH. and .OH, and the scavenging ability was dose dependent to some extent; the scavenging rates reached 91.15% and 89.06% respectively when the dose reached 4.0 mg/ml; the polysaccharides in yam bulbils significantly educed the blood glucose in model rice, and the hypoglycemic effect of large-dose polysaccharides was more obvious. The polysaccharides in yam bulbils has good antioxidant activity and hypoglycemic effect, which provides a new source for devel- opment of safe and natural food antioxidants and blood sugar-lowering agents.
基金supported by the National Natural Science Foundation of China(31960222)the Qinghai Provincial Major Science and Technology Special Funds(2023-NK-A3).
文摘Bistorta vivipara is a facultative reproductive plant capable of asexual reproduction through underground rhizomes and bulbils,as well as sexual reproduction via seeds.The phenomenon of vegetative organ vivipary is a complex biological process regulated by a network of genes.However,the developmental mechanism regulating bulbil vivipary in B.vivipara remains largely unexplored.This study investigated different developmental stages of B.vivipara using RNA sequencing and transcriptome analysis.Approximately 438 million high-quality reads were generated,with over 61.65%of the data mapped to the de novo transcriptome sequence.A total of 154,813 reads were matched in at least one public database,and 49,731 genes were differentially expressed across developmental stages.Functional analysis revealed significant enrichment of these genes in phenylpropanoid biosynthesis,plant hormone signal transduction,protein processing,starch and sucrose metabolism,and plant-pathogen interaction.Ninety-four genes involved in phytohormones,plant pigments,enzymes,and transcription factors were identified as potential candidates for inducing vegetative organ vivipary.These differentially expressed genes(DEGs),detected through comparative transcriptome analysis,may serve as candidate genes for bulbil vivipary in B.vivipara,establishing a foundation for future studies on the molecular mechanisms underlying vegetative organ vivipary.
文摘Aims Many polyploids are geographically separated from their lower-ploidy progenitors at a variety of spatial scales.Diverse adaptive and non-adaptive mechanisms have been suggested to contribute to these spatial patterns.A long-standing hypothesis holds that poly-ploids are established and persist due to specialization to differ-ent ecological conditions compared to their progenitors.However,experimental approaches testing this hypothesis remain rare.Empirical evidence for niche differences among ploidy levels has been found previously for the European polyploid geophyte Allium oleraceum.Here,we conducted a 5-year reciprocal transplant experiment with tetraploid,pentaploid and hexaploid A.oleraceum to test for adaptation at the ploidy and population levels.Methods In 2006,we sowed 2160 aerial bulbils among six sites(two per cytotype,central and western Moravia,the Czech Republic)and monitored their emergence in 2007,their survival until 2011 and their performance in 2011.Important Findings The emergence of bulbils was high at all target sites,and no evi-dence for niche differentiation among cytotypes was recorded in the first year.Survival in the following years was relatively high,but significantly better survival for plants of home ploidy than foreign ploidy was recorded.No plant reached the reproductive stage during our experiment.Adopting a strict definition of local adaptation,local plants only rarely performed better than foreign plants at both sites in pairwise comparisons within a cytotype.Together,our results sug-gest weak niche differentiation among cytotypes and provide limited support for the existence of local adaptation within cytotypes.Thus,all cytotypes are able to establish in habitats typically populated by other cytotypes via aerial bulbil spread,but will likely die out gradually in non-native habitats.This ability may explain the occur-rence of some multiple-cytotype populations.The complex spatial pattern of A.oleraceum cytotypes is therefore the result of several interacting factors,including niche differentiation among cytotypes,the different evolutionary ages of cytotypes,the existence of gene flow between and within cytotypes,the dominance of clonality and stochastic events linked to anthropogenic dispersal and disturbance.
