Belowground bud banks are essential for the regeneration of plant population in arid desert areas,and their response to environmental changes could reflect adaptive strategies of plants to desert habitats.However,the ...Belowground bud banks are essential for the regeneration of plant population in arid desert areas,and their response to environmental changes could reflect adaptive strategies of plants to desert habitats.However,the size and composition of belowground bud banks and their response to environmental factors in the desert steppe zone remain poorly understood,challenging desertification control efforts in arid desert areas.This study examined the density and vertical distribution of horizontal and vertical rhizome buds of a rhizomatous legume herb Sophora alopecuroides L.,its population characteristics,and soil physical-chemical properties in three habitats(interdune lowland(IL),flat sandy land(FSL),and desert steppe(DS))in a desert steppe zone,northern China.Our findings revealed that:(1)total and horizontal rhizome bud densities of S.alopecuroides differed significantly among the three habitats(P<0.05),with the largest total rhizome bud density(177 buds/m2)in IL and the smallest(63 buds/m2)in DS;(2)horizontal rhizome buds distributed in the deep soil layer were dominant in IL,while vertical rhizome buds in the top soil layer were predominant in DS;and(3)soil coarse sand,nutrient content,and population density were the primary factors affecting bud bank density of S.alopecuroides.Specifically,horizontal rhizome buds were dependent largely on soil coarse sand content,and vertical rhizome buds tended to be more related to soil organic matter content and population density.Our results indicated that horizontal rhizome buds were more important in IL with frequent aeolian disturbance,whereas vertical rhizome buds were more important in DS with abundant water and nutrient resources.The plastic responses and survival strategies of S.alopecuroides bud bank to different habitats provide valuable information for the effective implementation of desertification control measures and the management of desert steppe ecosystems.展开更多
Objective： To investigate the frequency of parasympathetic neurogenesis and determine its association with tumor budding and prognosis in pancreatic ductal adenocarcinoma （PDAC）. Methods： Parasympathetic neurogene...Objective： To investigate the frequency of parasympathetic neurogenesis and determine its association with tumor budding and prognosis in pancreatic ductal adenocarcinoma （PDAC）. Methods： Parasympathetic neurogenesis was defined as the distribution of abnormal parasympathetic nerves in the stroma tissue. Staining of vesicular acetylcholine transporter （VAChT）, as a marker for parasympathetic neurogenesis, was performed on a representative specimen of the tumor for 59 PDAC patients with available clinical, pathologic, and follow-up information. Three specimens containing normal pancreatic tissues were stained in parallel. The number of parasympathetic nerve fibers was counted in five high-power microscopic fields （5×0.785 mm2）. Cut-offvalues were calculated by receiver operating characteristic curve analysis. Results： VAChT-positive parasympathetic nerve fibers were not seen in the stroma of 3 cases of normal pancreatic tissues. In 59 PDAC cases, the range of parasympathetic neurogenesis was 4-38 fibers/（5×0.785） mm2, with a median of 18 fibers/（5×0.785） mm2. Patients with parasympathetic neurogenesis 〉 15 fibers/（5×0.785） mm2 were defined as the high-density group （39 patients, 66.1%）, and those with parasympathetic neurogenesis 〈15 fibers/（5×0.785） mm2 as the low-density group （20 patients, 33.9%）. The high-density group had a higher occurrence of tumor budding （P=0.001） and a higher rate of early recurrence （P=0.035）. Parasympathetic neurogenesis appeared to be an independent adverse prognostic factor [hazard ratio （HR）=2.45, 95% confidence interval （95% CI）： 1.25-4.81, P=0.009], in addition to American Joint Committee on Cancer （AJCC） stage （P=0.010） and tumor budding （P=0.009）. Conclusions： Parasympathetic neurogenesis is strongly associated with tumor budding and correlates with an adverse prognosis in PDAC.展开更多
From the dried flower-buds of Panax ginseng C.A. Meyer, a new ndnor dammaranetype triterpene saponin named ginsenoside III was iso1ated. On the basis of spectral and chemical evidence, the structure of the new saponin...From the dried flower-buds of Panax ginseng C.A. Meyer, a new ndnor dammaranetype triterpene saponin named ginsenoside III was iso1ated. On the basis of spectral and chemical evidence, the structure of the new saponin was elucidated as 3 -O- [β-D -glucopyranosyl (1→2 ) - βD- glucopyranosyl] - 20-O-β-D-glucopyranosyl 3 β, 12β- 20(S) -trihydroxydammar- 25 - en- 24-one.展开更多
Spring phenology is one of the most sensitive ecological indicators of forest responses to climate warming.Understanding the precise climatic drivers of bud break in keystone species is crucial for developing robust p...Spring phenology is one of the most sensitive ecological indicators of forest responses to climate warming.Understanding the precise climatic drivers of bud break in keystone species is crucial for developing robust phenological models and predicting future ecological and economic impacts.In this study,spring bud phenology was recorded 2020-2022 of 42 provenances of sugar maple(Acer saccharum Marsh.)originating from the northern range of the species in Quebec,Canada.The effect of temperature on budburst timing was assessed,and based on the observed linear relationship,we reconstructed the budburst timings of maple forests located between 45°and 49°N latitude and-70°and-76°W longitude over the past two decades.In the common garden the entire bud break process lasted between 20 and 40 d.Bud swelling occurred mid-April to mid-May,on average 5 d earlier in the southern and warmer stands.A strong correlation was observed between bud swelling dates and mean temperatures in the last two weeks of April,with temperature explaining 90%of the variance.An increase of 1℃in mean temperature during this period advanced budburst by 4 d.At the northern limit of sugar maple,late April had an average temperature between 1.6 and 8.7℃during 2003-2022,resulting in an estimated variability of 28 d in bud swelling from early April to early May.Our findings confirm that late April temperatures play a major role in the reactivation of sugar maple at its northern range.The earlier onset of leaf development under warming conditions could increase the risk of late frost damage,with consequences for maple syrup production and species distribution.展开更多
Cold tolerance is one of the important traits for grapevine,especially in regions with extremely low temperatures in winter.Vitis amurensis is wild species in the Vitis genus with excellent cold hardiness compared wit...Cold tolerance is one of the important traits for grapevine,especially in regions with extremely low temperatures in winter.Vitis amurensis is wild species in the Vitis genus with excellent cold hardiness compared with Vitis vinifera.However,metabolites that contribute to the cold tolerance of V.amurensis remain unknown.Here,the metabolomics of buds from V.amurensis‘Zuoshan-1'during cold acclimation(CA)were identified,and cold-sensitive cultivar(V.vinifera‘Jingzaojing')was used as the control.The buds were collected in October,November,and December in 2016 and 2018.The cold hardiness of the buds increased during CA in the two grapevines.However,browning was observed only in V.vinifera buds at temperature below-10℃.Among detected metabolites from buds,443 metabolites were overlapped between two years.Forty-four and thirty differentially accumulated metabolites(DAMs)were identified in V.amurensis and V.vinifera,respectively.Ten DAMs including monoacylglycerol(MAG,18:2)isomer 1,trehalose 6-phosphate,and D-glucose showed identical variations in the two grapevines,indicating conserved CA responses within the Vitis genus.Eighteen DAMs exhibited higher accumulation in V.amurensis than in V.vinifera.Maltotetraose,D-glucoronic acid,L-aspartic acid,azelaic acid,and 4-hydroxybenzoic acid were reported to accumulate during CA in other plants.Enhanced cold tolerance was detected in grapevine leaves with exogenous 5 mmol L^(-1)L-aspartic acid and 1%proanthocyanidins.Potential contributions of other DAMs found in V.amurensis such as Cyanidin 3-O-glucoside need to be further elucidated.Thus,eighteen metabolites accumulated in V.amurensis can be used for practical application in improvement of cold resistance in grapevine.Our findings provide new insights into understanding the cold hardiness of V.amurensis.展开更多
Bud dormancy represents a critical adaptation mechanism in perennial fruit plants,enabling survival during unfavorable environmental conditions.While this adaptive strategy is essential for plant survival and reproduc...Bud dormancy represents a critical adaptation mechanism in perennial fruit plants,enabling survival during unfavorable environmental conditions.While this adaptive strategy is essential for plant survival and reproduction,its molecular mechanisms remain incompletely understood.This study investigated two grape transcription factors:EARLY BUD BREAK(VvEBB)and SHORT VEGETATIVE PHASE 4(VvSVP4).Through heterologous transformation in poplar,the research demonstrated that VvSVP4 functions as a negative regulator of bud break,while VvEBB serves as a positive regulator.Transcriptome analysis revealed significant enrichment of plant hormone signaling pathways particularly ABA,IAA,and CK in plants overexpressing VvSVP4(VvSVP4-OE)and VvEBB(VvEBB-OE)compared to controls.Endogenous hormone level changes correlated positively with transcriptome data.During endodormancy,VvSVP4 directly and positively regulated the ABA receptor gene Vv PYL9 expression,maintaining bud dormancy.Conversely,during ecodormancy,rapid upregulation of VvEBB negatively regulated the expression of sucrose nonfermenting 1-related protein kinase 2 gene(Vv SAPK2),facilitating dormancy release.This study provides comprehensive insights into how VvSVP4 and VvEBB genes regulate dormancy and bud break through cell cycle regulation and multiple hormone signaling pathways.展开更多
Pancreatic ductal adenocarcinoma(PDAC)is the most prevalent type of pan-creatic neoplasm.It is a highly aggressive lethal malignancy related to its delayed in diagnosis and limited response to treatments.The incidence...Pancreatic ductal adenocarcinoma(PDAC)is the most prevalent type of pan-creatic neoplasm.It is a highly aggressive lethal malignancy related to its delayed in diagnosis and limited response to treatments.The incidence and mortality of pancreatic cancer have been increasing over the years.Tumor budding is a proven independent,adverse prognostic factor in PDAC.It is helpful for improvement of prognosis in PDAC in early and precise diagnostic modalities.Tumor budding should be conveyed in pathology reports and taken into account by future onco-logic staging systems.展开更多
Bud endodormancy represents an ecological adaptation mechanism in perennial deciduous fruit trees to endure winter cold conditions.Sucrose serves a crucial role in bud endodormancy as both an energy metabolizer and si...Bud endodormancy represents an ecological adaptation mechanism in perennial deciduous fruit trees to endure winter cold conditions.Sucrose serves a crucial role in bud endodormancy as both an energy metabolizer and signaling molecule.Sugars Will Eventually be Exported Transporters(SWEETs)function as sugar-efflux transporters that respond to environmental stimuli and contribute to plant growth and development.While SWEET gene families have been identified in various plant species for sugar transport regulation,their mechanism in regulating peach bud endodormancy remains undefined.In this study,we identified 15 SWEET genes in peach.The nomenclature was established through homologous alignment with the Arabidopsis SWEET gene family,resulting in four distinct clades through phylogenetic analysis.Covariance correlation analysis revealed 6 and 12 collinear SWEET genes in peach and Arabidopsis,respectively,forming 13 collinear gene pairs.Real-time quantitative polymerase chain reaction(RT-qPCR)analysis demonstrated significantly elevated expression of PpSWEET6 during peach bud endodormancy release,correlating positively with sucrose content.Transient overexpression of PpSWEET6 enhanced peach bud endodormancy release,while overexpressing PpSWEET6 in Arabidopsis enhanced seed germination and flowering.Y2H and luciferase complementation imaging(LCI)assays confirmed PpSWEET6 interacted with PpABF2.Additionally,dual luciferase reporter(DLR)assays showed that PpSWEET6 significantly decreased the activation of PpDAM6(key dormancy-inducing gene)through PpABF2,thereby modulating peach bud endodormancy release.These findings advance our understanding of SWEET genes in peach bud endodormancy regulation.展开更多
The Leymus chinensis grassland is one of themost widely distributed associations in the warmtemperate grassland and due to overgrazing in recent years,it has experienced varying degrees of degradation.Vegetative regen...The Leymus chinensis grassland is one of themost widely distributed associations in the warmtemperate grassland and due to overgrazing in recent years,it has experienced varying degrees of degradation.Vegetative regeneration via bud banks serves as the primaryway of vegetation reproduction in the L.chinensis grassland ecosystem.However,the role of the bud bank in the vegetation regeneration of grazing grassland remains unclear.Based on the relationship between the under-ground bud bank and above-ground vegetation of L.chinensis grassland under different grazing stages,this study aimed to explore whether the grazing grassland could self-recover through the existing bud bank.The findings revealed that the bud density initially increased and then decreased with increasing grazing intensity,indicating that appropriate grazing promoted vegetation renewal.Moreover,grazing significantly influenced the composition of the bud bank:during the early grazing stage,the rhizome buds accounted for the main part,and tiller buds dominated during the mid-stage grazing;while during the late-stage grazing,root-sprouting buds prevailed.The meristem restriction index for light,moderate,and heavy grazing grasslands was close to one;conversely,overgrazing and extreme overgrazing grasslands exhibited the highermeristemrestriction index(2.00,3.19),suggesting that plant regeneration was constrained by bud banks under light-grazing conditions where regenerate rates failed to meet above-ground modular’s recovery requirements following overgrazing and extreme overgrazing events.Consequently,moderate grazing grasslands could achieve natural community recovery by continuously adjusting their vegetative regeneration strategies.Understanding the role of bud banks in vegetative regeneration in grazing grassland will not only supply theoretical support for the ecological succession process of degraded grassland but also provide practical experience for the sustainable management of the L.chinensis grassland ecosystem.展开更多
This study aimed to investigate the mechanism underlying primary bud necrosis(PBN)in grapevines.PBN is a physiological disorder that significantly reduces grape yields.The four varieties,‘Shine Muscat’,‘Summer Blac...This study aimed to investigate the mechanism underlying primary bud necrosis(PBN)in grapevines.PBN is a physiological disorder that significantly reduces grape yields.The four varieties,‘Shine Muscat’,‘Summer Black’,‘Ruby Seedless’,and‘Hutai 8’,were investigated and found to exhibit differences in PBN,which was positively correlated with the speed and extent of inflorescence differentiation.Among them,‘Summer Black’was most susceptible to PBN.Treatment with gibberellin acid 3(GA_(3))notably accelerated and exacerbated PBN in‘Summer Black’,whereas the endogenous gibberellin(GA)inhibitor chlorocholine chloride(CCC)delayed or prevented PBN onset.Histological observations of dormant bud tissues revealed PBN progression in stages,starting with the expansion of cells in the necrosis zone(NZ),followed by cell wall irregularities and collapse,buckling cell layer formation,and subsequent cell separation.In the water control group,NZ mainly occurred in the bud scale layer.However,by the second week after GA_(3) treatment,primary buds visibly elongated,and NZ was formed at multiple locations along the primary buds.Transcriptomic analyses revealed significant regulation of stress-related genes,including reactive oxygen species(ROS)and heat-shock proteins(HSPs),following GA_(3) treatment.Genes related to jasmonic acid(JA)biosynthesis and signaling pathways were upregulated after week 2,whereas CCC treatment led to the downregulation of these genes.Furthermore,genes associated with cations such as calcium,iron,and copper showed significant changes across all transcriptome samples.Genes associated with the degradation of cell membranes and cell walls were upregulated in samples treated with GA_(3) and water control.Overall,these findings suggested that GA_(3) promoted PBN by enhancing JA synthesis and modulating the cell necrosis pathway via JA signaling.This process involved ROS accumulation and activation of cation pathways,leading to endomembrane and cell wall degradation,cell rupture,and,ultimately,PBN development.展开更多
BACKGROUND Although previous findings indicated that pathological assessment of tumor budding(TB),desmoplastic reaction(DR),and tumor-infiltrating lymphocytes(TILs)may play a role in determining tumor behavior in many...BACKGROUND Although previous findings indicated that pathological assessment of tumor budding(TB),desmoplastic reaction(DR),and tumor-infiltrating lymphocytes(TILs)may play a role in determining tumor behavior in many malignancies,the relationship between TB,DR,and TILs in patients with pancreatic ductal adenocarcinoma(PDAC)is still unknown.AIM To evaluate relationships of TB,DR,and TILs with histopathological parameters and determine their prognostic value in patients with PDAC.METHODS The study cohort comprised 100 patients diagnosed with PDAC.Peritumoral budding(PTB)and intratumoral budding(ITB)were assessed according to the International Tumor Budding Consensus Conference guidelines.DR was classified based on stromal maturation.TILs were evaluated semiquantitatively with a 5%cutoff.Additionally,cases were categorized into two groups according to lymphocyte density:No/Low lymphocytes and medium/high lymphocytes.RESULTS A significant correlation was observed between ITB and PTB(r=0.890).Higher PTB was associated with fewer TILs and immature stroma(P<0.001).PTB and TILs were significantly related to tumor dimension,lymphovascular invasion,lymph node metastasis(LNM),and stage(P<0.005).ITB was also associated with the presence of lymph node involvement.The results of the univariate analysis revealed a significant correlation between poor survival rates and the presence of lymphovascular invasion,LNM,PTB,ITB,and TILs according to scoring(P<0.001).The multivariate analysis revealed LNM,PTB,ITB,and TILs according to scoring as independent prognostic factors.CONCLUSION TB assessment stratified patients with PDAC.PTB-ITB correlation showed diagnostic relevance of ITB in biopsy specimens.The prognostic significance of DR and interplay with TIL subsets warrant further investigation.展开更多
To investigate endogenous hormone changes in“Hongyang”kiwifruit from overwintering buds to floral morphogenesis(bell-shaped flowering stage),systematic observations were conducted during the undifferentiated stage,a...To investigate endogenous hormone changes in“Hongyang”kiwifruit from overwintering buds to floral morphogenesis(bell-shaped flowering stage),systematic observations were conducted during the undifferentiated stage,axillary bud differentiation stage,and floral morphogenesis stage from late November 2023 to early April 2024.Paraffin sectioning was employed to examine floral bud morphology,while LC-MS targeted metabolomics quantified changes in 15 endogenous hormones across 8 classes.Results indicated floral bud differentiation commenced from late January to early February and concluded by mid-April,spanning approximately 70 days.Approximately 33 days after axillary bud initiation marked the axillary bud primordium differentiation stage,a critical phase for floral differentiation.The period from 33 to 61 days after axillary bud initiation constituted the key stage for female flower differentiation.Indole-3-acetic acid(IAA)levels remained consistently high and stable throughout the process.By the axillary bud primordium differentiation stage,abscisic acid(ABA),salicylic acid(SA),tylosterol(TY),and trans-zeatin riboside(tZR)levels significantly decrease,while 1-aminocyclopropane carboxylic acid(ACC),gibberellic acid(GA4),isopentenyladenine riboside(iPR),12-oxo-phytodienoic acid(12-OPDA),cis-zeatin(cZ),trans-zeatin(tZ),and cis-zeatin riboside(cZR)significantly increased.The study speculates that higher IAA levels may promote female flower differentiation;lower ABA levels favor floral bud differentiation;lower jasmonic acid(JA)levels may be associated with stamen abortion;lower SA levels favor female flower differentiation and development;and higher ACC and GA4 levels may promote female flower differentiation and flowering and subsequent development,respectively.展开更多
Genetic variation contributes to the phenotypic diversity of plants.Most red pear cultivars that accumulate anthocyanins originated from somatic variation and are termed as bud sports.‘Hongzaosu’is a bud sport deriv...Genetic variation contributes to the phenotypic diversity of plants.Most red pear cultivars that accumulate anthocyanins originated from somatic variation and are termed as bud sports.‘Hongzaosu’is a bud sport derived from the green pear‘Zaosu’.A new genetic map was constructed from a population derived from the cross of‘Yuluxiang’and‘Hongzaosu’,from which PpBBX24 was identified as a crucial gene controlling anthocyanin accumulation.Genetic and phylogenetic evidences revealed that PpBBX24 is a repressor of anthocyanin biosynthesis in pear.A 14 bp deletion was detected in the third exon of PpBBX24 in‘Hongzaosu’,resulting in premature termination of protein translation.The truncated PpBBX24 protein was a positive regulator of anthocyanin biosynthesis by activating the transcription of PpCHS and PpUFGT.Three independent variations in the BBX24 coding region that led to premature termination of translation were detected in other pear bud sports and the progeny of a bud sport that all accumulate anthocyanins.The genome of‘Hongzaosu’was assembled using both long-read and short-read sequences.By combining genomic and transcriptomic data,allele-specific expression of PpMYB110a was observed in the fruit skin of‘Hongzaosu',which was likely caused by a large variation in the promoter.This work enhances understanding of coloration in red pears and provides a novel genomic resource for further studies on‘Hongzaosu’pear.展开更多
This editorial discusses Alpsoy et al’s significant study of prognosis of pancreatic ductal adenocarcinoma(PDAC),which lacks histopathological markers.This study evaluated the synergistic prognolymphocytes.Peritumora...This editorial discusses Alpsoy et al’s significant study of prognosis of pancreatic ductal adenocarcinoma(PDAC),which lacks histopathological markers.This study evaluated the synergistic prognolymphocytes.Peritumoral budding is significantly correlated with tumor volume,while intratumoral budding is closely related to lymph node metastasis.Peritumoral budding and intratumoral budding are confirmed as independent adverse prognostic factors,and their high levels of expression are associated with immature stromal phenotypes,suggesting the key role of epithelial-mesenchymal transition.These breakthrough findings provide a new multidimensional biomarker system for the prognostic assessment of PDAC,and promote the clinical transformation process of incorporating tumor budding indicators into the pathological reporting process.However,the complexity and spatiotemporal heterogeneity of the tumor microenvironment require us to go beyond traditional morphological analysis and move towards multiomics integration and dynamic monitoring.Through standardized pathological assessment,innovative treatment strategies and interdisciplinary collaboration,it is expected to transform tumor microenvironment-related markers into clinically applicable indicators,ultimately improving the treatment predicament of PDAC.This editorial intended to summarize relevant studies and share some of our views,in order to offer perspectives for future research.展开更多
In grapevine,previous studies have suggested that,gibberellin(GA)inhibits bud break before dormancy release while enhanced after dormancy release;the capacity of GA accumulation shows a trend of first inhibition and t...In grapevine,previous studies have suggested that,gibberellin(GA)inhibits bud break before dormancy release while enhanced after dormancy release;the capacity of GA accumulation shows a trend of first inhibition and then upregulation.However,the regulatory mechanism of GA metabolism genes expression is as yet unclear during the process of dormancy release.In this study,we further validated the effect of GA3 and hydrogen cyanamide(HC)on bud break of‘Red Globe’grape,confirmed inhibition and promotion effect,respectively.Restricted GA biosynthetic genes'expression and enhanced GA catabolic gene's expression were observed in the early stage after HC treatment,while opposite expression trend showed in the late stage.VvSVP1,a MADS-box transcription factor gene,was downregulated in the late stage,which might play an important role in regulating GA metabolism genes'expression.It was shown that,VvSVP1 could bind to the promoter regions of GA biosynthetic gene VvGA20ox6 and catabolic gene VvGA2ox3,negatively and positively regulated the corresponding genes'expression,respectively;the contents of GAs related to GA20ox were significantly reduced in the grape callus overexpressed VvSVP1,while the ratio of GAs related to GA2ox were significantly increased.Taken together,VvSVP1 can regulate the endogenous GAs level by manipulating the expression of GA metabolism genes before dormant bud break induced by HC.Our findings may provide some new theoretical insights for the study of bud dormancy regulation in the perennial woody fruit trees.展开更多
基金funded by the National Natural Science Foundation of China(42207539,42377470)the Key Research and Development Project of Science and Technology Plan of Gansu Province in China International Science and Technology Cooperation Project(25YFWA009).
文摘Belowground bud banks are essential for the regeneration of plant population in arid desert areas,and their response to environmental changes could reflect adaptive strategies of plants to desert habitats.However,the size and composition of belowground bud banks and their response to environmental factors in the desert steppe zone remain poorly understood,challenging desertification control efforts in arid desert areas.This study examined the density and vertical distribution of horizontal and vertical rhizome buds of a rhizomatous legume herb Sophora alopecuroides L.,its population characteristics,and soil physical-chemical properties in three habitats(interdune lowland(IL),flat sandy land(FSL),and desert steppe(DS))in a desert steppe zone,northern China.Our findings revealed that:(1)total and horizontal rhizome bud densities of S.alopecuroides differed significantly among the three habitats(P<0.05),with the largest total rhizome bud density(177 buds/m2)in IL and the smallest(63 buds/m2)in DS;(2)horizontal rhizome buds distributed in the deep soil layer were dominant in IL,while vertical rhizome buds in the top soil layer were predominant in DS;and(3)soil coarse sand,nutrient content,and population density were the primary factors affecting bud bank density of S.alopecuroides.Specifically,horizontal rhizome buds were dependent largely on soil coarse sand content,and vertical rhizome buds tended to be more related to soil organic matter content and population density.Our results indicated that horizontal rhizome buds were more important in IL with frequent aeolian disturbance,whereas vertical rhizome buds were more important in DS with abundant water and nutrient resources.The plastic responses and survival strategies of S.alopecuroides bud bank to different habitats provide valuable information for the effective implementation of desertification control measures and the management of desert steppe ecosystems.
基金supported by grants from China Cancer Research Foundation Y-N2013-008the Doctoral Program of the Ministry of Education 20130001110089 to DR Xiu+1 种基金the National Natural Science Foundation of China 81272709 to W FuPeking University Third Hospital Grant Y81524-01 to LF Zhang
文摘Objective： To investigate the frequency of parasympathetic neurogenesis and determine its association with tumor budding and prognosis in pancreatic ductal adenocarcinoma （PDAC）. Methods： Parasympathetic neurogenesis was defined as the distribution of abnormal parasympathetic nerves in the stroma tissue. Staining of vesicular acetylcholine transporter （VAChT）, as a marker for parasympathetic neurogenesis, was performed on a representative specimen of the tumor for 59 PDAC patients with available clinical, pathologic, and follow-up information. Three specimens containing normal pancreatic tissues were stained in parallel. The number of parasympathetic nerve fibers was counted in five high-power microscopic fields （5×0.785 mm2）. Cut-offvalues were calculated by receiver operating characteristic curve analysis. Results： VAChT-positive parasympathetic nerve fibers were not seen in the stroma of 3 cases of normal pancreatic tissues. In 59 PDAC cases, the range of parasympathetic neurogenesis was 4-38 fibers/（5×0.785） mm2, with a median of 18 fibers/（5×0.785） mm2. Patients with parasympathetic neurogenesis 〉 15 fibers/（5×0.785） mm2 were defined as the high-density group （39 patients, 66.1%）, and those with parasympathetic neurogenesis 〈15 fibers/（5×0.785） mm2 as the low-density group （20 patients, 33.9%）. The high-density group had a higher occurrence of tumor budding （P=0.001） and a higher rate of early recurrence （P=0.035）. Parasympathetic neurogenesis appeared to be an independent adverse prognostic factor [hazard ratio （HR）=2.45, 95% confidence interval （95% CI）： 1.25-4.81, P=0.009], in addition to American Joint Committee on Cancer （AJCC） stage （P=0.010） and tumor budding （P=0.009）. Conclusions： Parasympathetic neurogenesis is strongly associated with tumor budding and correlates with an adverse prognosis in PDAC.
文摘From the dried flower-buds of Panax ginseng C.A. Meyer, a new ndnor dammaranetype triterpene saponin named ginsenoside III was iso1ated. On the basis of spectral and chemical evidence, the structure of the new saponin was elucidated as 3 -O- [β-D -glucopyranosyl (1→2 ) - βD- glucopyranosyl] - 20-O-β-D-glucopyranosyl 3 β, 12β- 20(S) -trihydroxydammar- 25 - en- 24-one.
基金supported by the Natural Sciences and Engineering Research Council of Canada(research programs Alliance and Discovery grants),the Fonds de Recherche du Québec-Sociétéet culture,the Ministère des Ressources naturelles et des Forêts du Québec,Syndicat des producteurs des bois du Saguenay-Lac-Saint-Jean,the Producteurs et productrices acéricoles du Québec,the Centre ACER,the Fondation de l’Universitédu QuébecàChicoutimisupported by the National Science Foundation of China(42301058)。
文摘Spring phenology is one of the most sensitive ecological indicators of forest responses to climate warming.Understanding the precise climatic drivers of bud break in keystone species is crucial for developing robust phenological models and predicting future ecological and economic impacts.In this study,spring bud phenology was recorded 2020-2022 of 42 provenances of sugar maple(Acer saccharum Marsh.)originating from the northern range of the species in Quebec,Canada.The effect of temperature on budburst timing was assessed,and based on the observed linear relationship,we reconstructed the budburst timings of maple forests located between 45°and 49°N latitude and-70°and-76°W longitude over the past two decades.In the common garden the entire bud break process lasted between 20 and 40 d.Bud swelling occurred mid-April to mid-May,on average 5 d earlier in the southern and warmer stands.A strong correlation was observed between bud swelling dates and mean temperatures in the last two weeks of April,with temperature explaining 90%of the variance.An increase of 1℃in mean temperature during this period advanced budburst by 4 d.At the northern limit of sugar maple,late April had an average temperature between 1.6 and 8.7℃during 2003-2022,resulting in an estimated variability of 28 d in bud swelling from early April to early May.Our findings confirm that late April temperatures play a major role in the reactivation of sugar maple at its northern range.The earlier onset of leaf development under warming conditions could increase the risk of late frost damage,with consequences for maple syrup production and species distribution.
基金supported by the National Key Research and Development Program of China(Grant No.2018YFD1000300)the National Natural Science Foundation of China(Grant Nos.32272691 and 32025032)+1 种基金the Grape Breeding Project of Ningxia(Grant No.NXNYYZ202101-04)the Major Program of Technological Innovation in Hubei Province(Grant No.2022BBA0022)。
文摘Cold tolerance is one of the important traits for grapevine,especially in regions with extremely low temperatures in winter.Vitis amurensis is wild species in the Vitis genus with excellent cold hardiness compared with Vitis vinifera.However,metabolites that contribute to the cold tolerance of V.amurensis remain unknown.Here,the metabolomics of buds from V.amurensis‘Zuoshan-1'during cold acclimation(CA)were identified,and cold-sensitive cultivar(V.vinifera‘Jingzaojing')was used as the control.The buds were collected in October,November,and December in 2016 and 2018.The cold hardiness of the buds increased during CA in the two grapevines.However,browning was observed only in V.vinifera buds at temperature below-10℃.Among detected metabolites from buds,443 metabolites were overlapped between two years.Forty-four and thirty differentially accumulated metabolites(DAMs)were identified in V.amurensis and V.vinifera,respectively.Ten DAMs including monoacylglycerol(MAG,18:2)isomer 1,trehalose 6-phosphate,and D-glucose showed identical variations in the two grapevines,indicating conserved CA responses within the Vitis genus.Eighteen DAMs exhibited higher accumulation in V.amurensis than in V.vinifera.Maltotetraose,D-glucoronic acid,L-aspartic acid,azelaic acid,and 4-hydroxybenzoic acid were reported to accumulate during CA in other plants.Enhanced cold tolerance was detected in grapevine leaves with exogenous 5 mmol L^(-1)L-aspartic acid and 1%proanthocyanidins.Potential contributions of other DAMs found in V.amurensis such as Cyanidin 3-O-glucoside need to be further elucidated.Thus,eighteen metabolites accumulated in V.amurensis can be used for practical application in improvement of cold resistance in grapevine.Our findings provide new insights into understanding the cold hardiness of V.amurensis.
基金supported by the China Agriculture Research System of MOF and MARA(CARS-29)the Xinjiang Uygur Autonomous Region Tianchi Talent Introduction Program,Chinathe Key R&D Technology Commissioner Projects in Hainan Province,China(ZDYF2024KJTPY008)。
文摘Bud dormancy represents a critical adaptation mechanism in perennial fruit plants,enabling survival during unfavorable environmental conditions.While this adaptive strategy is essential for plant survival and reproduction,its molecular mechanisms remain incompletely understood.This study investigated two grape transcription factors:EARLY BUD BREAK(VvEBB)and SHORT VEGETATIVE PHASE 4(VvSVP4).Through heterologous transformation in poplar,the research demonstrated that VvSVP4 functions as a negative regulator of bud break,while VvEBB serves as a positive regulator.Transcriptome analysis revealed significant enrichment of plant hormone signaling pathways particularly ABA,IAA,and CK in plants overexpressing VvSVP4(VvSVP4-OE)and VvEBB(VvEBB-OE)compared to controls.Endogenous hormone level changes correlated positively with transcriptome data.During endodormancy,VvSVP4 directly and positively regulated the ABA receptor gene Vv PYL9 expression,maintaining bud dormancy.Conversely,during ecodormancy,rapid upregulation of VvEBB negatively regulated the expression of sucrose nonfermenting 1-related protein kinase 2 gene(Vv SAPK2),facilitating dormancy release.This study provides comprehensive insights into how VvSVP4 and VvEBB genes regulate dormancy and bud break through cell cycle regulation and multiple hormone signaling pathways.
文摘Pancreatic ductal adenocarcinoma(PDAC)is the most prevalent type of pan-creatic neoplasm.It is a highly aggressive lethal malignancy related to its delayed in diagnosis and limited response to treatments.The incidence and mortality of pancreatic cancer have been increasing over the years.Tumor budding is a proven independent,adverse prognostic factor in PDAC.It is helpful for improvement of prognosis in PDAC in early and precise diagnostic modalities.Tumor budding should be conveyed in pathology reports and taken into account by future onco-logic staging systems.
基金funded by the National Key Research and Development Program of China(2018YFD1000104)the Shandong Provincial Fruit Industry Technology System Cultivation and Soil Fertilization Post,China(SDAIT-06-04)the Key R&D Program of Shandong Province(Competitive Innovation Platform),China(2022CXPT017).
文摘Bud endodormancy represents an ecological adaptation mechanism in perennial deciduous fruit trees to endure winter cold conditions.Sucrose serves a crucial role in bud endodormancy as both an energy metabolizer and signaling molecule.Sugars Will Eventually be Exported Transporters(SWEETs)function as sugar-efflux transporters that respond to environmental stimuli and contribute to plant growth and development.While SWEET gene families have been identified in various plant species for sugar transport regulation,their mechanism in regulating peach bud endodormancy remains undefined.In this study,we identified 15 SWEET genes in peach.The nomenclature was established through homologous alignment with the Arabidopsis SWEET gene family,resulting in four distinct clades through phylogenetic analysis.Covariance correlation analysis revealed 6 and 12 collinear SWEET genes in peach and Arabidopsis,respectively,forming 13 collinear gene pairs.Real-time quantitative polymerase chain reaction(RT-qPCR)analysis demonstrated significantly elevated expression of PpSWEET6 during peach bud endodormancy release,correlating positively with sucrose content.Transient overexpression of PpSWEET6 enhanced peach bud endodormancy release,while overexpressing PpSWEET6 in Arabidopsis enhanced seed germination and flowering.Y2H and luciferase complementation imaging(LCI)assays confirmed PpSWEET6 interacted with PpABF2.Additionally,dual luciferase reporter(DLR)assays showed that PpSWEET6 significantly decreased the activation of PpDAM6(key dormancy-inducing gene)through PpABF2,thereby modulating peach bud endodormancy release.These findings advance our understanding of SWEET genes in peach bud endodormancy regulation.
基金supported by the National Natural Science Foundation of China(42377458 and 41907411).
文摘The Leymus chinensis grassland is one of themost widely distributed associations in the warmtemperate grassland and due to overgrazing in recent years,it has experienced varying degrees of degradation.Vegetative regeneration via bud banks serves as the primaryway of vegetation reproduction in the L.chinensis grassland ecosystem.However,the role of the bud bank in the vegetation regeneration of grazing grassland remains unclear.Based on the relationship between the under-ground bud bank and above-ground vegetation of L.chinensis grassland under different grazing stages,this study aimed to explore whether the grazing grassland could self-recover through the existing bud bank.The findings revealed that the bud density initially increased and then decreased with increasing grazing intensity,indicating that appropriate grazing promoted vegetation renewal.Moreover,grazing significantly influenced the composition of the bud bank:during the early grazing stage,the rhizome buds accounted for the main part,and tiller buds dominated during the mid-stage grazing;while during the late-stage grazing,root-sprouting buds prevailed.The meristem restriction index for light,moderate,and heavy grazing grasslands was close to one;conversely,overgrazing and extreme overgrazing grasslands exhibited the highermeristemrestriction index(2.00,3.19),suggesting that plant regeneration was constrained by bud banks under light-grazing conditions where regenerate rates failed to meet above-ground modular’s recovery requirements following overgrazing and extreme overgrazing events.Consequently,moderate grazing grasslands could achieve natural community recovery by continuously adjusting their vegetative regeneration strategies.Understanding the role of bud banks in vegetative regeneration in grazing grassland will not only supply theoretical support for the ecological succession process of degraded grassland but also provide practical experience for the sustainable management of the L.chinensis grassland ecosystem.
基金supported by National Technology System for Grape Industry(Grant No.CARS-29-zp-9)National Key Research and Development Program of China(Grant No.2021YFD1200200)Natural Science Foundation of Hunan Province of China(Grant No.2023JJ50063).
文摘This study aimed to investigate the mechanism underlying primary bud necrosis(PBN)in grapevines.PBN is a physiological disorder that significantly reduces grape yields.The four varieties,‘Shine Muscat’,‘Summer Black’,‘Ruby Seedless’,and‘Hutai 8’,were investigated and found to exhibit differences in PBN,which was positively correlated with the speed and extent of inflorescence differentiation.Among them,‘Summer Black’was most susceptible to PBN.Treatment with gibberellin acid 3(GA_(3))notably accelerated and exacerbated PBN in‘Summer Black’,whereas the endogenous gibberellin(GA)inhibitor chlorocholine chloride(CCC)delayed or prevented PBN onset.Histological observations of dormant bud tissues revealed PBN progression in stages,starting with the expansion of cells in the necrosis zone(NZ),followed by cell wall irregularities and collapse,buckling cell layer formation,and subsequent cell separation.In the water control group,NZ mainly occurred in the bud scale layer.However,by the second week after GA_(3) treatment,primary buds visibly elongated,and NZ was formed at multiple locations along the primary buds.Transcriptomic analyses revealed significant regulation of stress-related genes,including reactive oxygen species(ROS)and heat-shock proteins(HSPs),following GA_(3) treatment.Genes related to jasmonic acid(JA)biosynthesis and signaling pathways were upregulated after week 2,whereas CCC treatment led to the downregulation of these genes.Furthermore,genes associated with cations such as calcium,iron,and copper showed significant changes across all transcriptome samples.Genes associated with the degradation of cell membranes and cell walls were upregulated in samples treated with GA_(3) and water control.Overall,these findings suggested that GA_(3) promoted PBN by enhancing JA synthesis and modulating the cell necrosis pathway via JA signaling.This process involved ROS accumulation and activation of cation pathways,leading to endomembrane and cell wall degradation,cell rupture,and,ultimately,PBN development.
文摘BACKGROUND Although previous findings indicated that pathological assessment of tumor budding(TB),desmoplastic reaction(DR),and tumor-infiltrating lymphocytes(TILs)may play a role in determining tumor behavior in many malignancies,the relationship between TB,DR,and TILs in patients with pancreatic ductal adenocarcinoma(PDAC)is still unknown.AIM To evaluate relationships of TB,DR,and TILs with histopathological parameters and determine their prognostic value in patients with PDAC.METHODS The study cohort comprised 100 patients diagnosed with PDAC.Peritumoral budding(PTB)and intratumoral budding(ITB)were assessed according to the International Tumor Budding Consensus Conference guidelines.DR was classified based on stromal maturation.TILs were evaluated semiquantitatively with a 5%cutoff.Additionally,cases were categorized into two groups according to lymphocyte density:No/Low lymphocytes and medium/high lymphocytes.RESULTS A significant correlation was observed between ITB and PTB(r=0.890).Higher PTB was associated with fewer TILs and immature stroma(P<0.001).PTB and TILs were significantly related to tumor dimension,lymphovascular invasion,lymph node metastasis(LNM),and stage(P<0.005).ITB was also associated with the presence of lymph node involvement.The results of the univariate analysis revealed a significant correlation between poor survival rates and the presence of lymphovascular invasion,LNM,PTB,ITB,and TILs according to scoring(P<0.001).The multivariate analysis revealed LNM,PTB,ITB,and TILs according to scoring as independent prognostic factors.CONCLUSION TB assessment stratified patients with PDAC.PTB-ITB correlation showed diagnostic relevance of ITB in biopsy specimens.The prognostic significance of DR and interplay with TIL subsets warrant further investigation.
基金funded by Sichuan Fruit Innovation Team Project(SCCXTD-2024-04)Sichuan Science and Technology Program(2024YFHZ0227,2025YFHZ0118,2023YFN0005).
文摘To investigate endogenous hormone changes in“Hongyang”kiwifruit from overwintering buds to floral morphogenesis(bell-shaped flowering stage),systematic observations were conducted during the undifferentiated stage,axillary bud differentiation stage,and floral morphogenesis stage from late November 2023 to early April 2024.Paraffin sectioning was employed to examine floral bud morphology,while LC-MS targeted metabolomics quantified changes in 15 endogenous hormones across 8 classes.Results indicated floral bud differentiation commenced from late January to early February and concluded by mid-April,spanning approximately 70 days.Approximately 33 days after axillary bud initiation marked the axillary bud primordium differentiation stage,a critical phase for floral differentiation.The period from 33 to 61 days after axillary bud initiation constituted the key stage for female flower differentiation.Indole-3-acetic acid(IAA)levels remained consistently high and stable throughout the process.By the axillary bud primordium differentiation stage,abscisic acid(ABA),salicylic acid(SA),tylosterol(TY),and trans-zeatin riboside(tZR)levels significantly decrease,while 1-aminocyclopropane carboxylic acid(ACC),gibberellic acid(GA4),isopentenyladenine riboside(iPR),12-oxo-phytodienoic acid(12-OPDA),cis-zeatin(cZ),trans-zeatin(tZ),and cis-zeatin riboside(cZR)significantly increased.The study speculates that higher IAA levels may promote female flower differentiation;lower ABA levels favor floral bud differentiation;lower jasmonic acid(JA)levels may be associated with stamen abortion;lower SA levels favor female flower differentiation and development;and higher ACC and GA4 levels may promote female flower differentiation and flowering and subsequent development,respectively.
基金supported by the Zhejiang Provincial Natural Science Foundation of China(LR22C150001 to SB,LY22C150003 to JN)the Specialized Research Fund for Major Science and Technique of Zhejiang Province of China(2021C02066-5)+1 种基金the National Natural Science Foundation of China(32072545 to YT,32272639 to SB)the China Agriculture Research System of MOF and MARA(CARS-28)and the Yunnan Science and Technology Talent and Platform Program(202205AF150041)。
文摘Genetic variation contributes to the phenotypic diversity of plants.Most red pear cultivars that accumulate anthocyanins originated from somatic variation and are termed as bud sports.‘Hongzaosu’is a bud sport derived from the green pear‘Zaosu’.A new genetic map was constructed from a population derived from the cross of‘Yuluxiang’and‘Hongzaosu’,from which PpBBX24 was identified as a crucial gene controlling anthocyanin accumulation.Genetic and phylogenetic evidences revealed that PpBBX24 is a repressor of anthocyanin biosynthesis in pear.A 14 bp deletion was detected in the third exon of PpBBX24 in‘Hongzaosu’,resulting in premature termination of protein translation.The truncated PpBBX24 protein was a positive regulator of anthocyanin biosynthesis by activating the transcription of PpCHS and PpUFGT.Three independent variations in the BBX24 coding region that led to premature termination of translation were detected in other pear bud sports and the progeny of a bud sport that all accumulate anthocyanins.The genome of‘Hongzaosu’was assembled using both long-read and short-read sequences.By combining genomic and transcriptomic data,allele-specific expression of PpMYB110a was observed in the fruit skin of‘Hongzaosu',which was likely caused by a large variation in the promoter.This work enhances understanding of coloration in red pears and provides a novel genomic resource for further studies on‘Hongzaosu’pear.
基金Supported by National Natural Science Foundation of China,No.82404058Shanghai Municipal Commission of Health and Family Planning,No.2024ZZ2049Beijing Xisike Clinical Oncology Research Foundation,No.Y-HS202401-0011.
文摘This editorial discusses Alpsoy et al’s significant study of prognosis of pancreatic ductal adenocarcinoma(PDAC),which lacks histopathological markers.This study evaluated the synergistic prognolymphocytes.Peritumoral budding is significantly correlated with tumor volume,while intratumoral budding is closely related to lymph node metastasis.Peritumoral budding and intratumoral budding are confirmed as independent adverse prognostic factors,and their high levels of expression are associated with immature stromal phenotypes,suggesting the key role of epithelial-mesenchymal transition.These breakthrough findings provide a new multidimensional biomarker system for the prognostic assessment of PDAC,and promote the clinical transformation process of incorporating tumor budding indicators into the pathological reporting process.However,the complexity and spatiotemporal heterogeneity of the tumor microenvironment require us to go beyond traditional morphological analysis and move towards multiomics integration and dynamic monitoring.Through standardized pathological assessment,innovative treatment strategies and interdisciplinary collaboration,it is expected to transform tumor microenvironment-related markers into clinically applicable indicators,ultimately improving the treatment predicament of PDAC.This editorial intended to summarize relevant studies and share some of our views,in order to offer perspectives for future research.
基金supported by the National Natural Science Foundation of China(Grant No.32172514).
文摘In grapevine,previous studies have suggested that,gibberellin(GA)inhibits bud break before dormancy release while enhanced after dormancy release;the capacity of GA accumulation shows a trend of first inhibition and then upregulation.However,the regulatory mechanism of GA metabolism genes expression is as yet unclear during the process of dormancy release.In this study,we further validated the effect of GA3 and hydrogen cyanamide(HC)on bud break of‘Red Globe’grape,confirmed inhibition and promotion effect,respectively.Restricted GA biosynthetic genes'expression and enhanced GA catabolic gene's expression were observed in the early stage after HC treatment,while opposite expression trend showed in the late stage.VvSVP1,a MADS-box transcription factor gene,was downregulated in the late stage,which might play an important role in regulating GA metabolism genes'expression.It was shown that,VvSVP1 could bind to the promoter regions of GA biosynthetic gene VvGA20ox6 and catabolic gene VvGA2ox3,negatively and positively regulated the corresponding genes'expression,respectively;the contents of GAs related to GA20ox were significantly reduced in the grape callus overexpressed VvSVP1,while the ratio of GAs related to GA2ox were significantly increased.Taken together,VvSVP1 can regulate the endogenous GAs level by manipulating the expression of GA metabolism genes before dormant bud break induced by HC.Our findings may provide some new theoretical insights for the study of bud dormancy regulation in the perennial woody fruit trees.
