Cold tolerance is one of the important traits for grapevine,especially in regions with extremely low temperatures in winter.Vitis amurensis is wild species in the Vitis genus with excellent cold hardiness compared wit...Cold tolerance is one of the important traits for grapevine,especially in regions with extremely low temperatures in winter.Vitis amurensis is wild species in the Vitis genus with excellent cold hardiness compared with Vitis vinifera.However,metabolites that contribute to the cold tolerance of V.amurensis remain unknown.Here,the metabolomics of buds from V.amurensis‘Zuoshan-1'during cold acclimation(CA)were identified,and cold-sensitive cultivar(V.vinifera‘Jingzaojing')was used as the control.The buds were collected in October,November,and December in 2016 and 2018.The cold hardiness of the buds increased during CA in the two grapevines.However,browning was observed only in V.vinifera buds at temperature below-10℃.Among detected metabolites from buds,443 metabolites were overlapped between two years.Forty-four and thirty differentially accumulated metabolites(DAMs)were identified in V.amurensis and V.vinifera,respectively.Ten DAMs including monoacylglycerol(MAG,18:2)isomer 1,trehalose 6-phosphate,and D-glucose showed identical variations in the two grapevines,indicating conserved CA responses within the Vitis genus.Eighteen DAMs exhibited higher accumulation in V.amurensis than in V.vinifera.Maltotetraose,D-glucoronic acid,L-aspartic acid,azelaic acid,and 4-hydroxybenzoic acid were reported to accumulate during CA in other plants.Enhanced cold tolerance was detected in grapevine leaves with exogenous 5 mmol L^(-1)L-aspartic acid and 1%proanthocyanidins.Potential contributions of other DAMs found in V.amurensis such as Cyanidin 3-O-glucoside need to be further elucidated.Thus,eighteen metabolites accumulated in V.amurensis can be used for practical application in improvement of cold resistance in grapevine.Our findings provide new insights into understanding the cold hardiness of V.amurensis.展开更多
Bud dormancy represents a critical adaptation mechanism in perennial fruit plants,enabling survival during unfavorable environmental conditions.While this adaptive strategy is essential for plant survival and reproduc...Bud dormancy represents a critical adaptation mechanism in perennial fruit plants,enabling survival during unfavorable environmental conditions.While this adaptive strategy is essential for plant survival and reproduction,its molecular mechanisms remain incompletely understood.This study investigated two grape transcription factors:EARLY BUD BREAK(VvEBB)and SHORT VEGETATIVE PHASE 4(VvSVP4).Through heterologous transformation in poplar,the research demonstrated that VvSVP4 functions as a negative regulator of bud break,while VvEBB serves as a positive regulator.Transcriptome analysis revealed significant enrichment of plant hormone signaling pathways particularly ABA,IAA,and CK in plants overexpressing VvSVP4(VvSVP4-OE)and VvEBB(VvEBB-OE)compared to controls.Endogenous hormone level changes correlated positively with transcriptome data.During endodormancy,VvSVP4 directly and positively regulated the ABA receptor gene Vv PYL9 expression,maintaining bud dormancy.Conversely,during ecodormancy,rapid upregulation of VvEBB negatively regulated the expression of sucrose nonfermenting 1-related protein kinase 2 gene(Vv SAPK2),facilitating dormancy release.This study provides comprehensive insights into how VvSVP4 and VvEBB genes regulate dormancy and bud break through cell cycle regulation and multiple hormone signaling pathways.展开更多
Pancreatic ductal adenocarcinoma(PDAC)is the most prevalent type of pan-creatic neoplasm.It is a highly aggressive lethal malignancy related to its delayed in diagnosis and limited response to treatments.The incidence...Pancreatic ductal adenocarcinoma(PDAC)is the most prevalent type of pan-creatic neoplasm.It is a highly aggressive lethal malignancy related to its delayed in diagnosis and limited response to treatments.The incidence and mortality of pancreatic cancer have been increasing over the years.Tumor budding is a proven independent,adverse prognostic factor in PDAC.It is helpful for improvement of prognosis in PDAC in early and precise diagnostic modalities.Tumor budding should be conveyed in pathology reports and taken into account by future onco-logic staging systems.展开更多
The sectioned thin cell layers (TCL) of flower stalk of Cichorium intybus L. were cultured in MS medium supplemented with NAA and BA or IAA and BA where floral and vegetative buds were developed from the explant. ...The sectioned thin cell layers (TCL) of flower stalk of Cichorium intybus L. were cultured in MS medium supplemented with NAA and BA or IAA and BA where floral and vegetative buds were developed from the explant. Endogenous IAA, DHZ+DHZR, iPA increased significantly during the floral bud formation, while Z+ZR remained changed. The levels of cytokinins, DHZ+DHZR, iPA, and Z+ZR all increased significantly during the vegetative bud formation, however IAA level was reduced during the first 7 days of culture and increased to two thirds of initial values on the day when the bud primordia were formed. The results suggested that the initiation of floral buds was associated with a high IAA/CTK ratio, whereas the induction of vegetative bud differentiation was related to a low IAA/CTK ratio.展开更多
Bud endodormancy represents an ecological adaptation mechanism in perennial deciduous fruit trees to endure winter cold conditions.Sucrose serves a crucial role in bud endodormancy as both an energy metabolizer and si...Bud endodormancy represents an ecological adaptation mechanism in perennial deciduous fruit trees to endure winter cold conditions.Sucrose serves a crucial role in bud endodormancy as both an energy metabolizer and signaling molecule.Sugars Will Eventually be Exported Transporters(SWEETs)function as sugar-efflux transporters that respond to environmental stimuli and contribute to plant growth and development.While SWEET gene families have been identified in various plant species for sugar transport regulation,their mechanism in regulating peach bud endodormancy remains undefined.In this study,we identified 15 SWEET genes in peach.The nomenclature was established through homologous alignment with the Arabidopsis SWEET gene family,resulting in four distinct clades through phylogenetic analysis.Covariance correlation analysis revealed 6 and 12 collinear SWEET genes in peach and Arabidopsis,respectively,forming 13 collinear gene pairs.Real-time quantitative polymerase chain reaction(RT-qPCR)analysis demonstrated significantly elevated expression of PpSWEET6 during peach bud endodormancy release,correlating positively with sucrose content.Transient overexpression of PpSWEET6 enhanced peach bud endodormancy release,while overexpressing PpSWEET6 in Arabidopsis enhanced seed germination and flowering.Y2H and luciferase complementation imaging(LCI)assays confirmed PpSWEET6 interacted with PpABF2.Additionally,dual luciferase reporter(DLR)assays showed that PpSWEET6 significantly decreased the activation of PpDAM6(key dormancy-inducing gene)through PpABF2,thereby modulating peach bud endodormancy release.These findings advance our understanding of SWEET genes in peach bud endodormancy regulation.展开更多
The Leymus chinensis grassland is one of themost widely distributed associations in the warmtemperate grassland and due to overgrazing in recent years,it has experienced varying degrees of degradation.Vegetative regen...The Leymus chinensis grassland is one of themost widely distributed associations in the warmtemperate grassland and due to overgrazing in recent years,it has experienced varying degrees of degradation.Vegetative regeneration via bud banks serves as the primaryway of vegetation reproduction in the L.chinensis grassland ecosystem.However,the role of the bud bank in the vegetation regeneration of grazing grassland remains unclear.Based on the relationship between the under-ground bud bank and above-ground vegetation of L.chinensis grassland under different grazing stages,this study aimed to explore whether the grazing grassland could self-recover through the existing bud bank.The findings revealed that the bud density initially increased and then decreased with increasing grazing intensity,indicating that appropriate grazing promoted vegetation renewal.Moreover,grazing significantly influenced the composition of the bud bank:during the early grazing stage,the rhizome buds accounted for the main part,and tiller buds dominated during the mid-stage grazing;while during the late-stage grazing,root-sprouting buds prevailed.The meristem restriction index for light,moderate,and heavy grazing grasslands was close to one;conversely,overgrazing and extreme overgrazing grasslands exhibited the highermeristemrestriction index(2.00,3.19),suggesting that plant regeneration was constrained by bud banks under light-grazing conditions where regenerate rates failed to meet above-ground modular’s recovery requirements following overgrazing and extreme overgrazing events.Consequently,moderate grazing grasslands could achieve natural community recovery by continuously adjusting their vegetative regeneration strategies.Understanding the role of bud banks in vegetative regeneration in grazing grassland will not only supply theoretical support for the ecological succession process of degraded grassland but also provide practical experience for the sustainable management of the L.chinensis grassland ecosystem.展开更多
BACKGROUND Although previous findings indicated that pathological assessment of tumor budding(TB),desmoplastic reaction(DR),and tumor-infiltrating lymphocytes(TILs)may play a role in determining tumor behavior in many...BACKGROUND Although previous findings indicated that pathological assessment of tumor budding(TB),desmoplastic reaction(DR),and tumor-infiltrating lymphocytes(TILs)may play a role in determining tumor behavior in many malignancies,the relationship between TB,DR,and TILs in patients with pancreatic ductal adenocarcinoma(PDAC)is still unknown.AIM To evaluate relationships of TB,DR,and TILs with histopathological parameters and determine their prognostic value in patients with PDAC.METHODS The study cohort comprised 100 patients diagnosed with PDAC.Peritumoral budding(PTB)and intratumoral budding(ITB)were assessed according to the International Tumor Budding Consensus Conference guidelines.DR was classified based on stromal maturation.TILs were evaluated semiquantitatively with a 5%cutoff.Additionally,cases were categorized into two groups according to lymphocyte density:No/Low lymphocytes and medium/high lymphocytes.RESULTS A significant correlation was observed between ITB and PTB(r=0.890).Higher PTB was associated with fewer TILs and immature stroma(P<0.001).PTB and TILs were significantly related to tumor dimension,lymphovascular invasion,lymph node metastasis(LNM),and stage(P<0.005).ITB was also associated with the presence of lymph node involvement.The results of the univariate analysis revealed a significant correlation between poor survival rates and the presence of lymphovascular invasion,LNM,PTB,ITB,and TILs according to scoring(P<0.001).The multivariate analysis revealed LNM,PTB,ITB,and TILs according to scoring as independent prognostic factors.CONCLUSION TB assessment stratified patients with PDAC.PTB-ITB correlation showed diagnostic relevance of ITB in biopsy specimens.The prognostic significance of DR and interplay with TIL subsets warrant further investigation.展开更多
This study aimed to investigate the mechanism underlying primary bud necrosis(PBN)in grapevines.PBN is a physiological disorder that significantly reduces grape yields.The four varieties,‘Shine Muscat’,‘Summer Blac...This study aimed to investigate the mechanism underlying primary bud necrosis(PBN)in grapevines.PBN is a physiological disorder that significantly reduces grape yields.The four varieties,‘Shine Muscat’,‘Summer Black’,‘Ruby Seedless’,and‘Hutai 8’,were investigated and found to exhibit differences in PBN,which was positively correlated with the speed and extent of inflorescence differentiation.Among them,‘Summer Black’was most susceptible to PBN.Treatment with gibberellin acid 3(GA_(3))notably accelerated and exacerbated PBN in‘Summer Black’,whereas the endogenous gibberellin(GA)inhibitor chlorocholine chloride(CCC)delayed or prevented PBN onset.Histological observations of dormant bud tissues revealed PBN progression in stages,starting with the expansion of cells in the necrosis zone(NZ),followed by cell wall irregularities and collapse,buckling cell layer formation,and subsequent cell separation.In the water control group,NZ mainly occurred in the bud scale layer.However,by the second week after GA_(3) treatment,primary buds visibly elongated,and NZ was formed at multiple locations along the primary buds.Transcriptomic analyses revealed significant regulation of stress-related genes,including reactive oxygen species(ROS)and heat-shock proteins(HSPs),following GA_(3) treatment.Genes related to jasmonic acid(JA)biosynthesis and signaling pathways were upregulated after week 2,whereas CCC treatment led to the downregulation of these genes.Furthermore,genes associated with cations such as calcium,iron,and copper showed significant changes across all transcriptome samples.Genes associated with the degradation of cell membranes and cell walls were upregulated in samples treated with GA_(3) and water control.Overall,these findings suggested that GA_(3) promoted PBN by enhancing JA synthesis and modulating the cell necrosis pathway via JA signaling.This process involved ROS accumulation and activation of cation pathways,leading to endomembrane and cell wall degradation,cell rupture,and,ultimately,PBN development.展开更多
In grapevine,previous studies have suggested that,gibberellin(GA)inhibits bud break before dormancy release while enhanced after dormancy release;the capacity of GA accumulation shows a trend of first inhibition and t...In grapevine,previous studies have suggested that,gibberellin(GA)inhibits bud break before dormancy release while enhanced after dormancy release;the capacity of GA accumulation shows a trend of first inhibition and then upregulation.However,the regulatory mechanism of GA metabolism genes expression is as yet unclear during the process of dormancy release.In this study,we further validated the effect of GA3 and hydrogen cyanamide(HC)on bud break of‘Red Globe’grape,confirmed inhibition and promotion effect,respectively.Restricted GA biosynthetic genes'expression and enhanced GA catabolic gene's expression were observed in the early stage after HC treatment,while opposite expression trend showed in the late stage.VvSVP1,a MADS-box transcription factor gene,was downregulated in the late stage,which might play an important role in regulating GA metabolism genes'expression.It was shown that,VvSVP1 could bind to the promoter regions of GA biosynthetic gene VvGA20ox6 and catabolic gene VvGA2ox3,negatively and positively regulated the corresponding genes'expression,respectively;the contents of GAs related to GA20ox were significantly reduced in the grape callus overexpressed VvSVP1,while the ratio of GAs related to GA2ox were significantly increased.Taken together,VvSVP1 can regulate the endogenous GAs level by manipulating the expression of GA metabolism genes before dormant bud break induced by HC.Our findings may provide some new theoretical insights for the study of bud dormancy regulation in the perennial woody fruit trees.展开更多
Genetic variation contributes to the phenotypic diversity of plants.Most red pear cultivars that accumulate anthocyanins originated from somatic variation and are termed as bud sports.‘Hongzaosu’is a bud sport deriv...Genetic variation contributes to the phenotypic diversity of plants.Most red pear cultivars that accumulate anthocyanins originated from somatic variation and are termed as bud sports.‘Hongzaosu’is a bud sport derived from the green pear‘Zaosu’.A new genetic map was constructed from a population derived from the cross of‘Yuluxiang’and‘Hongzaosu’,from which PpBBX24 was identified as a crucial gene controlling anthocyanin accumulation.Genetic and phylogenetic evidences revealed that PpBBX24 is a repressor of anthocyanin biosynthesis in pear.A 14 bp deletion was detected in the third exon of PpBBX24 in‘Hongzaosu’,resulting in premature termination of protein translation.The truncated PpBBX24 protein was a positive regulator of anthocyanin biosynthesis by activating the transcription of PpCHS and PpUFGT.Three independent variations in the BBX24 coding region that led to premature termination of translation were detected in other pear bud sports and the progeny of a bud sport that all accumulate anthocyanins.The genome of‘Hongzaosu’was assembled using both long-read and short-read sequences.By combining genomic and transcriptomic data,allele-specific expression of PpMYB110a was observed in the fruit skin of‘Hongzaosu',which was likely caused by a large variation in the promoter.This work enhances understanding of coloration in red pears and provides a novel genomic resource for further studies on‘Hongzaosu’pear.展开更多
This editorial discusses Alpsoy et al’s significant study of prognosis of pancreatic ductal adenocarcinoma(PDAC),which lacks histopathological markers.This study evaluated the synergistic prognolymphocytes.Peritumora...This editorial discusses Alpsoy et al’s significant study of prognosis of pancreatic ductal adenocarcinoma(PDAC),which lacks histopathological markers.This study evaluated the synergistic prognolymphocytes.Peritumoral budding is significantly correlated with tumor volume,while intratumoral budding is closely related to lymph node metastasis.Peritumoral budding and intratumoral budding are confirmed as independent adverse prognostic factors,and their high levels of expression are associated with immature stromal phenotypes,suggesting the key role of epithelial-mesenchymal transition.These breakthrough findings provide a new multidimensional biomarker system for the prognostic assessment of PDAC,and promote the clinical transformation process of incorporating tumor budding indicators into the pathological reporting process.However,the complexity and spatiotemporal heterogeneity of the tumor microenvironment require us to go beyond traditional morphological analysis and move towards multiomics integration and dynamic monitoring.Through standardized pathological assessment,innovative treatment strategies and interdisciplinary collaboration,it is expected to transform tumor microenvironment-related markers into clinically applicable indicators,ultimately improving the treatment predicament of PDAC.This editorial intended to summarize relevant studies and share some of our views,in order to offer perspectives for future research.展开更多
The drought resistances of 44 maize germplasms at budding stage were studied with 19.2% polyethylene glycol(PEG-6000)in order to provide new germplasm resource for drought resistance breeding of maize.The results show...The drought resistances of 44 maize germplasms at budding stage were studied with 19.2% polyethylene glycol(PEG-6000)in order to provide new germplasm resource for drought resistance breeding of maize.The results showed there were five extremely high drought resistance germplasms,20 germplasms with high drought resistance,and 19 germplasms with medium drought resistance.The significance testing was also used to demonstrate that the germination index(GI)and germination of drought resistance index(GDRI)of N4,N54-1 and Wuming No.1 were dramatically remarkable than others.The conclusion was that the 44 maize germplasm had good drought resistance and could be used as germplasm resource of the drought resistance breeding of maize,which had a great value of development and utilization.展开更多
Aim To study the chemical constituents of the flower buds of Tussilago farfara L. in the China National GAP Base of Traditional Chinese Materia Medica and provide scientific basis for quality control. Methods The cons...Aim To study the chemical constituents of the flower buds of Tussilago farfara L. in the China National GAP Base of Traditional Chinese Materia Medica and provide scientific basis for quality control. Methods The constituents were separated and purified by different chromatographic methods, and their structures were elucidated by IR, MS and NMR techniques. Results Twenty eight compounds were isolated from the flower buds of T. farfara. Their structures were identified as n- heptacosane （1）, bis（2-ethylhexyl）phthalate （2）, 7β-[3＇-ethylcrotonoyloxy]-1α-[2＇-methylbutyryloxy]-3,14-dehydro-Z-notonipetranone （3）, 7β-[3＇-ethylcrotonoyloxy]-1α-[2＇-methylbutyryloxy]-3,14-dehydro-E-notonipetranone （4）, tussilagone （5）, dibutyl phthalate （6）, bauer-7-ene-3β,16α-diol （7）, isobauerenol （8）, stigmasterol （9）, β-sitosterol （10）, 2,2-dimethyl-6-acetylchromanone （11）, n- hexadecanoic acid （12）, 7β-hydroxysitosterol （13）, 7α-hydroxysitosterol （14）, 7,14-bisdesacylnotonipetrone （15）, 2,3- dihydroxypropylpalmitate （16）, daucosterol （17）, 6-hydroxy-2,6-dimethylhept-2-en-4-one （18）, ferulic acid （19）, isoferulic acid （20）, caffeic acid （21）, α-D-glucose （22）, sucrose （23）, phthalic acid （24）, p-hydroxybenzoic acid （25）, gallic acid （26）, uridine （27）, and adenosine （28）. Conclusion Compounds 1, 12-16, 18 and 20 were obtained from the genus Tussilago for the first time.展开更多
[Objective] We aimed to investigate the preventive and therapeutical effect of compound of traditional Chinese drugs （Japanese raisintree fruit, lobed kudzuvine flower bud and lightyel ow sophora root） on acute alc...[Objective] We aimed to investigate the preventive and therapeutical effect of compound of traditional Chinese drugs （Japanese raisintree fruit, lobed kudzuvine flower bud and lightyel ow sophora root） on acute alcohol intoxication in mice. [Method] Acute alcohol intoxication was induced by administering alcohol to mice. Three different doses （low, middle and high） of compound of traditional Chinese drugs were administered to mice before and after administering alcohol respectively to investigate the preventive and therapeutical effect of drugs on acute alcohol intox-ication through doing statistical analysis about drunk mice and their sleeping time. The concentration of malondialdehyde （MDA）, reduced glutathione （GSH） and triglyc-erides （TG） in liver was also determined to investigate the protective effect of drugs on liver. [Result] The efficacy of compound of traditional Chinese drugs on acute al-cohol intoxication was dose-dependent. High-dose administration decreased the number of drunk mice significantly compared with control group; middle- and high-dose administration reduced the sleeping time of drunk mice and the concentration of MDA and TG in liver tissue; three doses al increased the concentration of GSH. [Conclusion] The compound of Japanese raisintree fruit, lobed kudzuvine flower bud and lightyel ow sophora root had preventive and therapeutical effect on hangover, and it also had certain preventive and therapeutical effect on liver damage caused by alcohol.展开更多
By introducing the theory of suburb urbanization,it was considered that budding villages in cities emerged with the close relation between city and suburb during rapid urbanization process.The paper had briefly analyz...By introducing the theory of suburb urbanization,it was considered that budding villages in cities emerged with the close relation between city and suburb during rapid urbanization process.The paper had briefly analyzed development of villages in cities in China.By taking villages in Lanzhou City for example,it had studied geological location,appearance,spatial structure,economy and demographic feature of budding villages in cities.Furthermore,with Chaijiahe Village of Xiguoyuan Town as case study,empirical study had been conducted and development thought of Chaijiahe Village as budding village in city had been discussed.It emphasized that it should control the development scale of villages,economically and intensively utilize lands;develop industrialized high-efficiency agriculture and leisure agriculture,develop lily economy and explore the potential of characteristic industries;realize integration of urban and rural development;strive to develop education,improve villages' quality and cultivate villagers of new generation who possessed pioneering spirit and modern agriculture technology.On this basis,it summarized approaches to realize sustainable development of budding villages in cities.展开更多
[Objective] This study was conducted to change the situation of high cost in walnut grafting by reducing seedling cost through technical means.[Method] Effects of different rootstock processing methods and rootstock p...[Objective] This study was conducted to change the situation of high cost in walnut grafting by reducing seedling cost through technical means.[Method] Effects of different rootstock processing methods and rootstock pruning time on walnut growth and cost were studied by the square bud grafting method.[Result] The survival rate and cost under stumping were higher than those under no stumping,and the proportion of grafting-capable rootstocks under stumping was lower than that under no stumping by 9%.The cost differences between different rootstock time were remarkable.The cost for the stock pruning 15 d after grafting was 5 times as much as 1 d after grafting.[Conclusion] Direct grafting could achieve the effect of easy and convenient management,and the overall cost was lower though the survival rate was reduced to a limited extent.Sooner rootstock pruning resulted in a better effect.Pruning the rootstocks within 5 d after grafting not only could improve the quality of seedlings,but also could reduce the cost for seedling management.展开更多
Three main parameters were selected to study their importance in transformation by budmicroinjection in non-head Chinese cabbage [Brassica campestris ssp. chinensis (L.) Makinovar. communis Tsen et Lee]. The results s...Three main parameters were selected to study their importance in transformation by budmicroinjection in non-head Chinese cabbage [Brassica campestris ssp. chinensis (L.) Makinovar. communis Tsen et Lee]. The results showed that the developmental stage of floral bud, theconcentrations of sucrose and surfactant Silwet L-77 were critical for the successfultransformation by this new method. The suitable bud size is 2-3 mm in length, the favorableconcentration of sucrose and surfactant Silwet L-77 are 8 and 0.02% respectively. When thesucrose concentration was greater than 10% or that of Silwet L-77 was above 0.1%, the treatedbuds became yellow and finally blighted. 4/6 T1 seedlings resistant to kanamycin were positiveby PCR analysis, and T2 progeny of all these positive T1 plants have one or more hybridizingbands by Southern blot. Under 5% sucrose, 0.02% Silwet L-77 and grade 2 bud (2-3 mm in itslength) parameters, the most favorable transformation efficiency is about 0.56%, and meanefficiency reaches 0.16% in all experiments indicating that bud microinjection is potentialtransformation way in non-head Chinese cabbage.展开更多
[Objective] The aim was to study the relationship of plant characters with bud living rate and mother stem emergence rate of ratoon rice before harvest. [Method] Eighteen mid-season hybrid rice cultivators approved re...[Objective] The aim was to study the relationship of plant characters with bud living rate and mother stem emergence rate of ratoon rice before harvest. [Method] Eighteen mid-season hybrid rice cultivators approved recently were taken as materials and relationship of plant characters including bud living rate and moth- er stem emergence rate of ratoon rice before harvest was explored, based on relat- ed data. [Result] The shorter rice with lower harvesting index is the main character of high emergence rate for first cropping rice; decline of plant height and increase of ear-bearing percentage would improve emergence of regenerative seedlings. [Conclusion] The research provided scientific reference for breeding of rice cultivars with high regenerative capacity.展开更多
[Objective] This study aimed to investigate the endodormancy release in nectarine bud treated by short-term freezing. [Method] Through short-term freezing at seven different temperatures for three different periods on...[Objective] This study aimed to investigate the endodormancy release in nectarine bud treated by short-term freezing. [Method] Through short-term freezing at seven different temperatures for three different periods on bud, the livability, burst, ratio of free water to bound water and membrane permeability of ＇Shuguang＇ nec- tarine bud were studied. [Result] On November 10, compared with non-freezing treatment （CK）, the bud burst, ratio of free water to bound water and membrane permeability treated by freezing at -5 and -8 ℃ were almost the same as CK. But the rest freezing treatments advanced the date of endodormancy release, while the bud burst, ratio of free water to bound water and membrane permeability were high- er than CK. on November 20 and 30, the effects of the freezing treatment on en- dodormancy release were the same when the treatment on November 10, and the effect was better as the treatment was later. [Conclusion] The correlation of the rate of bud burst, ratio of free water to bound water, and membrane permeability of the different freezing treatments indicated that the change from bound water to free wa- ter and the increase of membrane permeability were probably the signal of endodor- mancy release.展开更多
基金supported by the National Key Research and Development Program of China(Grant No.2018YFD1000300)the National Natural Science Foundation of China(Grant Nos.32272691 and 32025032)+1 种基金the Grape Breeding Project of Ningxia(Grant No.NXNYYZ202101-04)the Major Program of Technological Innovation in Hubei Province(Grant No.2022BBA0022)。
文摘Cold tolerance is one of the important traits for grapevine,especially in regions with extremely low temperatures in winter.Vitis amurensis is wild species in the Vitis genus with excellent cold hardiness compared with Vitis vinifera.However,metabolites that contribute to the cold tolerance of V.amurensis remain unknown.Here,the metabolomics of buds from V.amurensis‘Zuoshan-1'during cold acclimation(CA)were identified,and cold-sensitive cultivar(V.vinifera‘Jingzaojing')was used as the control.The buds were collected in October,November,and December in 2016 and 2018.The cold hardiness of the buds increased during CA in the two grapevines.However,browning was observed only in V.vinifera buds at temperature below-10℃.Among detected metabolites from buds,443 metabolites were overlapped between two years.Forty-four and thirty differentially accumulated metabolites(DAMs)were identified in V.amurensis and V.vinifera,respectively.Ten DAMs including monoacylglycerol(MAG,18:2)isomer 1,trehalose 6-phosphate,and D-glucose showed identical variations in the two grapevines,indicating conserved CA responses within the Vitis genus.Eighteen DAMs exhibited higher accumulation in V.amurensis than in V.vinifera.Maltotetraose,D-glucoronic acid,L-aspartic acid,azelaic acid,and 4-hydroxybenzoic acid were reported to accumulate during CA in other plants.Enhanced cold tolerance was detected in grapevine leaves with exogenous 5 mmol L^(-1)L-aspartic acid and 1%proanthocyanidins.Potential contributions of other DAMs found in V.amurensis such as Cyanidin 3-O-glucoside need to be further elucidated.Thus,eighteen metabolites accumulated in V.amurensis can be used for practical application in improvement of cold resistance in grapevine.Our findings provide new insights into understanding the cold hardiness of V.amurensis.
基金supported by the China Agriculture Research System of MOF and MARA(CARS-29)the Xinjiang Uygur Autonomous Region Tianchi Talent Introduction Program,Chinathe Key R&D Technology Commissioner Projects in Hainan Province,China(ZDYF2024KJTPY008)。
文摘Bud dormancy represents a critical adaptation mechanism in perennial fruit plants,enabling survival during unfavorable environmental conditions.While this adaptive strategy is essential for plant survival and reproduction,its molecular mechanisms remain incompletely understood.This study investigated two grape transcription factors:EARLY BUD BREAK(VvEBB)and SHORT VEGETATIVE PHASE 4(VvSVP4).Through heterologous transformation in poplar,the research demonstrated that VvSVP4 functions as a negative regulator of bud break,while VvEBB serves as a positive regulator.Transcriptome analysis revealed significant enrichment of plant hormone signaling pathways particularly ABA,IAA,and CK in plants overexpressing VvSVP4(VvSVP4-OE)and VvEBB(VvEBB-OE)compared to controls.Endogenous hormone level changes correlated positively with transcriptome data.During endodormancy,VvSVP4 directly and positively regulated the ABA receptor gene Vv PYL9 expression,maintaining bud dormancy.Conversely,during ecodormancy,rapid upregulation of VvEBB negatively regulated the expression of sucrose nonfermenting 1-related protein kinase 2 gene(Vv SAPK2),facilitating dormancy release.This study provides comprehensive insights into how VvSVP4 and VvEBB genes regulate dormancy and bud break through cell cycle regulation and multiple hormone signaling pathways.
文摘Pancreatic ductal adenocarcinoma(PDAC)is the most prevalent type of pan-creatic neoplasm.It is a highly aggressive lethal malignancy related to its delayed in diagnosis and limited response to treatments.The incidence and mortality of pancreatic cancer have been increasing over the years.Tumor budding is a proven independent,adverse prognostic factor in PDAC.It is helpful for improvement of prognosis in PDAC in early and precise diagnostic modalities.Tumor budding should be conveyed in pathology reports and taken into account by future onco-logic staging systems.
文摘The sectioned thin cell layers (TCL) of flower stalk of Cichorium intybus L. were cultured in MS medium supplemented with NAA and BA or IAA and BA where floral and vegetative buds were developed from the explant. Endogenous IAA, DHZ+DHZR, iPA increased significantly during the floral bud formation, while Z+ZR remained changed. The levels of cytokinins, DHZ+DHZR, iPA, and Z+ZR all increased significantly during the vegetative bud formation, however IAA level was reduced during the first 7 days of culture and increased to two thirds of initial values on the day when the bud primordia were formed. The results suggested that the initiation of floral buds was associated with a high IAA/CTK ratio, whereas the induction of vegetative bud differentiation was related to a low IAA/CTK ratio.
基金funded by the National Key Research and Development Program of China(2018YFD1000104)the Shandong Provincial Fruit Industry Technology System Cultivation and Soil Fertilization Post,China(SDAIT-06-04)the Key R&D Program of Shandong Province(Competitive Innovation Platform),China(2022CXPT017).
文摘Bud endodormancy represents an ecological adaptation mechanism in perennial deciduous fruit trees to endure winter cold conditions.Sucrose serves a crucial role in bud endodormancy as both an energy metabolizer and signaling molecule.Sugars Will Eventually be Exported Transporters(SWEETs)function as sugar-efflux transporters that respond to environmental stimuli and contribute to plant growth and development.While SWEET gene families have been identified in various plant species for sugar transport regulation,their mechanism in regulating peach bud endodormancy remains undefined.In this study,we identified 15 SWEET genes in peach.The nomenclature was established through homologous alignment with the Arabidopsis SWEET gene family,resulting in four distinct clades through phylogenetic analysis.Covariance correlation analysis revealed 6 and 12 collinear SWEET genes in peach and Arabidopsis,respectively,forming 13 collinear gene pairs.Real-time quantitative polymerase chain reaction(RT-qPCR)analysis demonstrated significantly elevated expression of PpSWEET6 during peach bud endodormancy release,correlating positively with sucrose content.Transient overexpression of PpSWEET6 enhanced peach bud endodormancy release,while overexpressing PpSWEET6 in Arabidopsis enhanced seed germination and flowering.Y2H and luciferase complementation imaging(LCI)assays confirmed PpSWEET6 interacted with PpABF2.Additionally,dual luciferase reporter(DLR)assays showed that PpSWEET6 significantly decreased the activation of PpDAM6(key dormancy-inducing gene)through PpABF2,thereby modulating peach bud endodormancy release.These findings advance our understanding of SWEET genes in peach bud endodormancy regulation.
基金supported by the National Natural Science Foundation of China(42377458 and 41907411).
文摘The Leymus chinensis grassland is one of themost widely distributed associations in the warmtemperate grassland and due to overgrazing in recent years,it has experienced varying degrees of degradation.Vegetative regeneration via bud banks serves as the primaryway of vegetation reproduction in the L.chinensis grassland ecosystem.However,the role of the bud bank in the vegetation regeneration of grazing grassland remains unclear.Based on the relationship between the under-ground bud bank and above-ground vegetation of L.chinensis grassland under different grazing stages,this study aimed to explore whether the grazing grassland could self-recover through the existing bud bank.The findings revealed that the bud density initially increased and then decreased with increasing grazing intensity,indicating that appropriate grazing promoted vegetation renewal.Moreover,grazing significantly influenced the composition of the bud bank:during the early grazing stage,the rhizome buds accounted for the main part,and tiller buds dominated during the mid-stage grazing;while during the late-stage grazing,root-sprouting buds prevailed.The meristem restriction index for light,moderate,and heavy grazing grasslands was close to one;conversely,overgrazing and extreme overgrazing grasslands exhibited the highermeristemrestriction index(2.00,3.19),suggesting that plant regeneration was constrained by bud banks under light-grazing conditions where regenerate rates failed to meet above-ground modular’s recovery requirements following overgrazing and extreme overgrazing events.Consequently,moderate grazing grasslands could achieve natural community recovery by continuously adjusting their vegetative regeneration strategies.Understanding the role of bud banks in vegetative regeneration in grazing grassland will not only supply theoretical support for the ecological succession process of degraded grassland but also provide practical experience for the sustainable management of the L.chinensis grassland ecosystem.
文摘BACKGROUND Although previous findings indicated that pathological assessment of tumor budding(TB),desmoplastic reaction(DR),and tumor-infiltrating lymphocytes(TILs)may play a role in determining tumor behavior in many malignancies,the relationship between TB,DR,and TILs in patients with pancreatic ductal adenocarcinoma(PDAC)is still unknown.AIM To evaluate relationships of TB,DR,and TILs with histopathological parameters and determine their prognostic value in patients with PDAC.METHODS The study cohort comprised 100 patients diagnosed with PDAC.Peritumoral budding(PTB)and intratumoral budding(ITB)were assessed according to the International Tumor Budding Consensus Conference guidelines.DR was classified based on stromal maturation.TILs were evaluated semiquantitatively with a 5%cutoff.Additionally,cases were categorized into two groups according to lymphocyte density:No/Low lymphocytes and medium/high lymphocytes.RESULTS A significant correlation was observed between ITB and PTB(r=0.890).Higher PTB was associated with fewer TILs and immature stroma(P<0.001).PTB and TILs were significantly related to tumor dimension,lymphovascular invasion,lymph node metastasis(LNM),and stage(P<0.005).ITB was also associated with the presence of lymph node involvement.The results of the univariate analysis revealed a significant correlation between poor survival rates and the presence of lymphovascular invasion,LNM,PTB,ITB,and TILs according to scoring(P<0.001).The multivariate analysis revealed LNM,PTB,ITB,and TILs according to scoring as independent prognostic factors.CONCLUSION TB assessment stratified patients with PDAC.PTB-ITB correlation showed diagnostic relevance of ITB in biopsy specimens.The prognostic significance of DR and interplay with TIL subsets warrant further investigation.
基金supported by National Technology System for Grape Industry(Grant No.CARS-29-zp-9)National Key Research and Development Program of China(Grant No.2021YFD1200200)Natural Science Foundation of Hunan Province of China(Grant No.2023JJ50063).
文摘This study aimed to investigate the mechanism underlying primary bud necrosis(PBN)in grapevines.PBN is a physiological disorder that significantly reduces grape yields.The four varieties,‘Shine Muscat’,‘Summer Black’,‘Ruby Seedless’,and‘Hutai 8’,were investigated and found to exhibit differences in PBN,which was positively correlated with the speed and extent of inflorescence differentiation.Among them,‘Summer Black’was most susceptible to PBN.Treatment with gibberellin acid 3(GA_(3))notably accelerated and exacerbated PBN in‘Summer Black’,whereas the endogenous gibberellin(GA)inhibitor chlorocholine chloride(CCC)delayed or prevented PBN onset.Histological observations of dormant bud tissues revealed PBN progression in stages,starting with the expansion of cells in the necrosis zone(NZ),followed by cell wall irregularities and collapse,buckling cell layer formation,and subsequent cell separation.In the water control group,NZ mainly occurred in the bud scale layer.However,by the second week after GA_(3) treatment,primary buds visibly elongated,and NZ was formed at multiple locations along the primary buds.Transcriptomic analyses revealed significant regulation of stress-related genes,including reactive oxygen species(ROS)and heat-shock proteins(HSPs),following GA_(3) treatment.Genes related to jasmonic acid(JA)biosynthesis and signaling pathways were upregulated after week 2,whereas CCC treatment led to the downregulation of these genes.Furthermore,genes associated with cations such as calcium,iron,and copper showed significant changes across all transcriptome samples.Genes associated with the degradation of cell membranes and cell walls were upregulated in samples treated with GA_(3) and water control.Overall,these findings suggested that GA_(3) promoted PBN by enhancing JA synthesis and modulating the cell necrosis pathway via JA signaling.This process involved ROS accumulation and activation of cation pathways,leading to endomembrane and cell wall degradation,cell rupture,and,ultimately,PBN development.
基金supported by the National Natural Science Foundation of China(Grant No.32172514).
文摘In grapevine,previous studies have suggested that,gibberellin(GA)inhibits bud break before dormancy release while enhanced after dormancy release;the capacity of GA accumulation shows a trend of first inhibition and then upregulation.However,the regulatory mechanism of GA metabolism genes expression is as yet unclear during the process of dormancy release.In this study,we further validated the effect of GA3 and hydrogen cyanamide(HC)on bud break of‘Red Globe’grape,confirmed inhibition and promotion effect,respectively.Restricted GA biosynthetic genes'expression and enhanced GA catabolic gene's expression were observed in the early stage after HC treatment,while opposite expression trend showed in the late stage.VvSVP1,a MADS-box transcription factor gene,was downregulated in the late stage,which might play an important role in regulating GA metabolism genes'expression.It was shown that,VvSVP1 could bind to the promoter regions of GA biosynthetic gene VvGA20ox6 and catabolic gene VvGA2ox3,negatively and positively regulated the corresponding genes'expression,respectively;the contents of GAs related to GA20ox were significantly reduced in the grape callus overexpressed VvSVP1,while the ratio of GAs related to GA2ox were significantly increased.Taken together,VvSVP1 can regulate the endogenous GAs level by manipulating the expression of GA metabolism genes before dormant bud break induced by HC.Our findings may provide some new theoretical insights for the study of bud dormancy regulation in the perennial woody fruit trees.
基金supported by the Zhejiang Provincial Natural Science Foundation of China(LR22C150001 to SB,LY22C150003 to JN)the Specialized Research Fund for Major Science and Technique of Zhejiang Province of China(2021C02066-5)+1 种基金the National Natural Science Foundation of China(32072545 to YT,32272639 to SB)the China Agriculture Research System of MOF and MARA(CARS-28)and the Yunnan Science and Technology Talent and Platform Program(202205AF150041)。
文摘Genetic variation contributes to the phenotypic diversity of plants.Most red pear cultivars that accumulate anthocyanins originated from somatic variation and are termed as bud sports.‘Hongzaosu’is a bud sport derived from the green pear‘Zaosu’.A new genetic map was constructed from a population derived from the cross of‘Yuluxiang’and‘Hongzaosu’,from which PpBBX24 was identified as a crucial gene controlling anthocyanin accumulation.Genetic and phylogenetic evidences revealed that PpBBX24 is a repressor of anthocyanin biosynthesis in pear.A 14 bp deletion was detected in the third exon of PpBBX24 in‘Hongzaosu’,resulting in premature termination of protein translation.The truncated PpBBX24 protein was a positive regulator of anthocyanin biosynthesis by activating the transcription of PpCHS and PpUFGT.Three independent variations in the BBX24 coding region that led to premature termination of translation were detected in other pear bud sports and the progeny of a bud sport that all accumulate anthocyanins.The genome of‘Hongzaosu’was assembled using both long-read and short-read sequences.By combining genomic and transcriptomic data,allele-specific expression of PpMYB110a was observed in the fruit skin of‘Hongzaosu',which was likely caused by a large variation in the promoter.This work enhances understanding of coloration in red pears and provides a novel genomic resource for further studies on‘Hongzaosu’pear.
基金Supported by National Natural Science Foundation of China,No.82404058Shanghai Municipal Commission of Health and Family Planning,No.2024ZZ2049Beijing Xisike Clinical Oncology Research Foundation,No.Y-HS202401-0011.
文摘This editorial discusses Alpsoy et al’s significant study of prognosis of pancreatic ductal adenocarcinoma(PDAC),which lacks histopathological markers.This study evaluated the synergistic prognolymphocytes.Peritumoral budding is significantly correlated with tumor volume,while intratumoral budding is closely related to lymph node metastasis.Peritumoral budding and intratumoral budding are confirmed as independent adverse prognostic factors,and their high levels of expression are associated with immature stromal phenotypes,suggesting the key role of epithelial-mesenchymal transition.These breakthrough findings provide a new multidimensional biomarker system for the prognostic assessment of PDAC,and promote the clinical transformation process of incorporating tumor budding indicators into the pathological reporting process.However,the complexity and spatiotemporal heterogeneity of the tumor microenvironment require us to go beyond traditional morphological analysis and move towards multiomics integration and dynamic monitoring.Through standardized pathological assessment,innovative treatment strategies and interdisciplinary collaboration,it is expected to transform tumor microenvironment-related markers into clinically applicable indicators,ultimately improving the treatment predicament of PDAC.This editorial intended to summarize relevant studies and share some of our views,in order to offer perspectives for future research.
基金Supported by the Subproject of National Science Foundation Platform Project(2005DKA21000-5-49)~~
文摘The drought resistances of 44 maize germplasms at budding stage were studied with 19.2% polyethylene glycol(PEG-6000)in order to provide new germplasm resource for drought resistance breeding of maize.The results showed there were five extremely high drought resistance germplasms,20 germplasms with high drought resistance,and 19 germplasms with medium drought resistance.The significance testing was also used to demonstrate that the germination index(GI)and germination of drought resistance index(GDRI)of N4,N54-1 and Wuming No.1 were dramatically remarkable than others.The conclusion was that the 44 maize germplasm had good drought resistance and could be used as germplasm resource of the drought resistance breeding of maize,which had a great value of development and utilization.
基金The National High-Tech"863"Project(Grant No.2004AA2Z3730-07)State Projects of the Tenth-Five-year Plan(Grant No.2001-BA701A62-11).
文摘Aim To study the chemical constituents of the flower buds of Tussilago farfara L. in the China National GAP Base of Traditional Chinese Materia Medica and provide scientific basis for quality control. Methods The constituents were separated and purified by different chromatographic methods, and their structures were elucidated by IR, MS and NMR techniques. Results Twenty eight compounds were isolated from the flower buds of T. farfara. Their structures were identified as n- heptacosane （1）, bis（2-ethylhexyl）phthalate （2）, 7β-[3＇-ethylcrotonoyloxy]-1α-[2＇-methylbutyryloxy]-3,14-dehydro-Z-notonipetranone （3）, 7β-[3＇-ethylcrotonoyloxy]-1α-[2＇-methylbutyryloxy]-3,14-dehydro-E-notonipetranone （4）, tussilagone （5）, dibutyl phthalate （6）, bauer-7-ene-3β,16α-diol （7）, isobauerenol （8）, stigmasterol （9）, β-sitosterol （10）, 2,2-dimethyl-6-acetylchromanone （11）, n- hexadecanoic acid （12）, 7β-hydroxysitosterol （13）, 7α-hydroxysitosterol （14）, 7,14-bisdesacylnotonipetrone （15）, 2,3- dihydroxypropylpalmitate （16）, daucosterol （17）, 6-hydroxy-2,6-dimethylhept-2-en-4-one （18）, ferulic acid （19）, isoferulic acid （20）, caffeic acid （21）, α-D-glucose （22）, sucrose （23）, phthalic acid （24）, p-hydroxybenzoic acid （25）, gallic acid （26）, uridine （27）, and adenosine （28）. Conclusion Compounds 1, 12-16, 18 and 20 were obtained from the genus Tussilago for the first time.
基金Supported by National Natural Science Foundation of China(31100987)Project of Shandong University of Technology(4040-306018)Young Teacher Development Plan of Shandong University of Technology~~
文摘[Objective] We aimed to investigate the preventive and therapeutical effect of compound of traditional Chinese drugs （Japanese raisintree fruit, lobed kudzuvine flower bud and lightyel ow sophora root） on acute alcohol intoxication in mice. [Method] Acute alcohol intoxication was induced by administering alcohol to mice. Three different doses （low, middle and high） of compound of traditional Chinese drugs were administered to mice before and after administering alcohol respectively to investigate the preventive and therapeutical effect of drugs on acute alcohol intox-ication through doing statistical analysis about drunk mice and their sleeping time. The concentration of malondialdehyde （MDA）, reduced glutathione （GSH） and triglyc-erides （TG） in liver was also determined to investigate the protective effect of drugs on liver. [Result] The efficacy of compound of traditional Chinese drugs on acute al-cohol intoxication was dose-dependent. High-dose administration decreased the number of drunk mice significantly compared with control group; middle- and high-dose administration reduced the sleeping time of drunk mice and the concentration of MDA and TG in liver tissue; three doses al increased the concentration of GSH. [Conclusion] The compound of Japanese raisintree fruit, lobed kudzuvine flower bud and lightyel ow sophora root had preventive and therapeutical effect on hangover, and it also had certain preventive and therapeutical effect on liver damage caused by alcohol.
基金Supported by Youth Foundation of Zhengzhou Institute of Aeronautical Industry Management(Q08JZ007)~~
文摘By introducing the theory of suburb urbanization,it was considered that budding villages in cities emerged with the close relation between city and suburb during rapid urbanization process.The paper had briefly analyzed development of villages in cities in China.By taking villages in Lanzhou City for example,it had studied geological location,appearance,spatial structure,economy and demographic feature of budding villages in cities.Furthermore,with Chaijiahe Village of Xiguoyuan Town as case study,empirical study had been conducted and development thought of Chaijiahe Village as budding village in city had been discussed.It emphasized that it should control the development scale of villages,economically and intensively utilize lands;develop industrialized high-efficiency agriculture and leisure agriculture,develop lily economy and explore the potential of characteristic industries;realize integration of urban and rural development;strive to develop education,improve villages' quality and cultivate villagers of new generation who possessed pioneering spirit and modern agriculture technology.On this basis,it summarized approaches to realize sustainable development of budding villages in cities.
文摘[Objective] This study was conducted to change the situation of high cost in walnut grafting by reducing seedling cost through technical means.[Method] Effects of different rootstock processing methods and rootstock pruning time on walnut growth and cost were studied by the square bud grafting method.[Result] The survival rate and cost under stumping were higher than those under no stumping,and the proportion of grafting-capable rootstocks under stumping was lower than that under no stumping by 9%.The cost differences between different rootstock time were remarkable.The cost for the stock pruning 15 d after grafting was 5 times as much as 1 d after grafting.[Conclusion] Direct grafting could achieve the effect of easy and convenient management,and the overall cost was lower though the survival rate was reduced to a limited extent.Sooner rootstock pruning resulted in a better effect.Pruning the rootstocks within 5 d after grafting not only could improve the quality of seedlings,but also could reduce the cost for seedling management.
文摘Three main parameters were selected to study their importance in transformation by budmicroinjection in non-head Chinese cabbage [Brassica campestris ssp. chinensis (L.) Makinovar. communis Tsen et Lee]. The results showed that the developmental stage of floral bud, theconcentrations of sucrose and surfactant Silwet L-77 were critical for the successfultransformation by this new method. The suitable bud size is 2-3 mm in length, the favorableconcentration of sucrose and surfactant Silwet L-77 are 8 and 0.02% respectively. When thesucrose concentration was greater than 10% or that of Silwet L-77 was above 0.1%, the treatedbuds became yellow and finally blighted. 4/6 T1 seedlings resistant to kanamycin were positiveby PCR analysis, and T2 progeny of all these positive T1 plants have one or more hybridizingbands by Southern blot. Under 5% sucrose, 0.02% Silwet L-77 and grade 2 bud (2-3 mm in itslength) parameters, the most favorable transformation efficiency is about 0.56%, and meanefficiency reaches 0.16% in all experiments indicating that bud microinjection is potentialtransformation way in non-head Chinese cabbage.
基金Supported by Sichuan Financial and Engineering Foundation for the Young(2007QNJJ-020)Sichuan Cultivation Foundation for Academy leaders+3 种基金Sichuan Key Project of Rice BreedingConstruction of Rice Industry System in SouthwestNational Crop-harvesting ProjectSichuan Financial Promotion~~
文摘[Objective] The aim was to study the relationship of plant characters with bud living rate and mother stem emergence rate of ratoon rice before harvest. [Method] Eighteen mid-season hybrid rice cultivators approved recently were taken as materials and relationship of plant characters including bud living rate and moth- er stem emergence rate of ratoon rice before harvest was explored, based on relat- ed data. [Result] The shorter rice with lower harvesting index is the main character of high emergence rate for first cropping rice; decline of plant height and increase of ear-bearing percentage would improve emergence of regenerative seedlings. [Conclusion] The research provided scientific reference for breeding of rice cultivars with high regenerative capacity.
基金Supported by the Special Fund of Modern System of Agricultural Industry Technology(CARS-30-6)the Special Fund of Department of Science and Technology,Liaoning Province(2011204001)~~
文摘[Objective] This study aimed to investigate the endodormancy release in nectarine bud treated by short-term freezing. [Method] Through short-term freezing at seven different temperatures for three different periods on bud, the livability, burst, ratio of free water to bound water and membrane permeability of ＇Shuguang＇ nec- tarine bud were studied. [Result] On November 10, compared with non-freezing treatment （CK）, the bud burst, ratio of free water to bound water and membrane permeability treated by freezing at -5 and -8 ℃ were almost the same as CK. But the rest freezing treatments advanced the date of endodormancy release, while the bud burst, ratio of free water to bound water and membrane permeability were high- er than CK. on November 20 and 30, the effects of the freezing treatment on en- dodormancy release were the same when the treatment on November 10, and the effect was better as the treatment was later. [Conclusion] The correlation of the rate of bud burst, ratio of free water to bound water, and membrane permeability of the different freezing treatments indicated that the change from bound water to free wa- ter and the increase of membrane permeability were probably the signal of endodor- mancy release.
