Hyperlipidemia constitutes a critical factor in the pathogenesis of vascular disease and gut microbiota dysbiosis.This study aims to investigate the role of sea buckthorn fruit oil(SBFO)in ameliorating hyperlipidemia,...Hyperlipidemia constitutes a critical factor in the pathogenesis of vascular disease and gut microbiota dysbiosis.This study aims to investigate the role of sea buckthorn fruit oil(SBFO)in ameliorating hyperlipidemia,modulating gut microbiota,and elucidating the underlying mechanisms of its lipid-lowering effects.SBFO intervention in hyperlipidemic mice significantly reduced body weight and visceral adiposity index while mitigating symptoms associated with hyperlipidemia,including dyslipidemia,infla mmation,liver damage,and endothelial dysfunction.Real-time quantitative PCR and Western blot analyses revealed that SBFO enhanced the response of free fatty acid oxidation and breakdown(adenosine 5'-monophosphateactivated protein kinaseα,carnitine palmitoyltransferase1a,peroxisome proliferator-activated receptorα,and peroxisome proliferator-activated receptorγ)while suppressing the synthesis of triglycerides and fatty acids(sterol-regulatory element binding protein 1,fatty acid synthase,and acetyl CoA carboxylase)at the gene and protein expression levels.Moreover,SBFO intervention enriched the gut microbiota of hyperlipidemic mice by increasing the relative abundances of Lactobacillus,Faecalibaculum,and Allobaculum,while decreasing the Firmicutes/Bacteroidetes ratio.These findings suggest that SBFO exhibits promising therapeutic potential in attenuating hyperlipidemia in mice through the regulation of lipid metabolism and modulation of gut microbiota.展开更多
Sea buckthorn(Hippophae rhamnoides L.)is a natural homologous substance of medicine and food.Polysaccharide,as one of its primary active components,has very superior biological activity and can be used as a dietary su...Sea buckthorn(Hippophae rhamnoides L.)is a natural homologous substance of medicine and food.Polysaccharide,as one of its primary active components,has very superior biological activity and can be used as a dietary supplement for functional foods,with good commercial prospects.Although initial progress has been made in the study of sea buckthorn polysaccharides,related studies have been fragmented and lacked systematic and generalization.This manuscript presents a critical analysis and systematic summary of the extraction and purification methods,structural characterization and physicochemical properties,biological activity and potential mechanisms,and structure-activity relationships of sea buckthorn polysaccharides.Accumulating evidence has indicated that sea buckthorn polysaccharides,which were widely prepared by water extraction and column chromatography purifications,exhibited exhibit superior biological activities in vitro and in vivo,including antioxidant,immunomodulatory,anti-inflammatory,hepatorenal protective,antibacterial,antiviral,and prebiotic activities.After analysis,it was concluded that there is a correlation between the relevant activities of sea buckthorn polysaccharides and that the structure of sea buckthorn polysaccharides has a great influence on their biological activity.We reviewed the challenges and limitations of sea buckthorn polysaccharides,summarized the critical aspects,and provided suggestions for potential breakthroughs in the research and application of sea buckthorn polysaccharide.展开更多
The aim of this study was to explore the mechanism of action of sea buckthorn polyphenols in the treatment of hyperlipidemia through network pharmacology and molecular docking.The TCMSP pharmacology database was used ...The aim of this study was to explore the mechanism of action of sea buckthorn polyphenols in the treatment of hyperlipidemia through network pharmacology and molecular docking.The TCMSP pharmacology database was used to screen the polyphenols present in sea buckthorn,and then the SwissTargetPrediction and Uniprot databases were used to obtain the potential targets of sea buckthorn polyphenols,which were supplemented by the literature.In total,7 polyphenols and 154 potential targets were obtained.Through GeneCards,OMIM database,1358 hyperlipidemia-related targets were collected.We found that there were 101 targets at the intersection of components and diseases.Through GO and KEGG enrichment analysis,27 core targets were obtained,which were AKT1,TNF,TP53,IL-6,etc.in order of degree value.174 pathways were obtained from KEGG enrichment analysis,including AGE-RAGE signaling pathway in diabetic complications,fl uid shear stress and atherosclerosis,lipid and atherosclerosis,etc.The molecular docking of the main components to the targets was performed using OpenBabelGUI,AutoDockTools-1.5.6 software.Finally,the results were visualized using Cytoscape 3.9.1 software.The molecular docking results showed that sea buckthorn polyphenols have good binding ability with the key targets.Among them,such as quercetin and kaempferol,have good binding ability with TNF,TP53 and IL-6.For example,TNF binds to quercetin with a binding energy of-5.34 kcal/mol and to kaempferol with a binding energy of-6.22 kcal/mol;TP53 binds to kaempferol with a binding energy of-5.32 kcal/mol;IL-6 binds to quercetin with a binding energy of-5.62 kcal/mol,etc.Therefore,the network pharmacology study showed that the treatment of hyperlipidemia by sea buckthorn polyphenols can be realized by multi-component-multi-target-multi-pathway together,which provides some reference for the later study of sea buckthorn polyphenols in the treatment of hyperlipidemia.展开更多
Background:Seabuckthorn is a widely distributed,high-yielding shrub valued for its medicinal and nutritional properties,with its fruit rich in flavonoids and polysaccharides.While studies have focused on the anti-infl...Background:Seabuckthorn is a widely distributed,high-yielding shrub valued for its medicinal and nutritional properties,with its fruit rich in flavonoids and polysaccharides.While studies have focused on the anti-inflammatory,antioxidant,anticancer,and immune-boosting effects of these compounds,research on their skincare benefits is limited.Additionally,there is a lack of method development and characterization of their actual skincare efficacy.Objective:The main objective of this study was to investigate the antimicrobial,whitening,moisturizing,and restorative effects of seabuckthorn polysaccharides and flavonoids.An innovative gel leave-on mask was developed with these active ingredients,and its stability and skincare efficacy were evaluated through characterization experiments focusing on antibacterial,whitening,moisturizing,and restorative effects.Materials and Methods:Seabuckthorn polysaccharides and flavonoids were extracted using both aqueous and alcoholic methods.The antibacterial,antioxidant and whitening efficacy of sea buckthorn flavonoids was investigated by the antibacterial test of Propionibacterium acnes,ABTS free radical scavenging test and tyrosinase inhibition test.An innovative gel leave-on mask formula was developed,incorporating glycerol glucoside and hydrolyzed collagen for added moisturizing and reparative benefits.The stability of the gel mask was tested for pH stability,ionic strength stability,and thermodynamic stability using measurements of particle size,polydispersity index(PDI),and zeta potential.Finally,the practical efficacy of the gel leave-on mask was evaluated through participant trials,measuring skin parameters such as vascular density,skin roughness,moisture content,and pigmentation.Results:The study demonstrated that seabuckthorn flavonoids exhibited excellent antimicrobial,anti-inflammatory and whitening effects.The gel leave-on mask formulation proved stable.Participants showed significant improvements in skin parameters,including reduced roughness,increased moisture content,and enhanced pigmentation balance,confirming the practical efficacy of the product.Conclusion:In conclusion,this study fills the gap in the systematic verification of the multifaceted skincare benefits of seabuckthorn flavonoids and polysaccharides,while also developing a product that efficiently integrates these two active ingredients.The formulation's practical efficacy was confirmed,showing significant effects in antibacterial,anti-inflammatory,and whitening applications.展开更多
In this study,the structural characters,antioxidant activities and bile acid-binding ability of sea buckthorn polysaccharides(HRPs)obtained by the commonly used hot water(HRP-W),pressurized hot water(HRP-H),ultrasonic...In this study,the structural characters,antioxidant activities and bile acid-binding ability of sea buckthorn polysaccharides(HRPs)obtained by the commonly used hot water(HRP-W),pressurized hot water(HRP-H),ultrasonic(HRP-U),acid(HRP-C)and alkali(HRP-A)assisted extraction methods were investigated.The results demonstrated that extraction methods had significant effects on extraction yield,monosaccharide composition,molecular weight,particle size,triple-helical structure,and surface morphology of HRPs except for the major linkage bands.Thermogravimetric analysis showed that HRP-U with filamentous reticular microstructure exhibited better thermal stability.The HRP-A with the lowest molecular weight and highest arabinose content possessed the best antioxidant activities.Moreover,the rheological analysis indicated that HRPs with higher galacturonic acid content and molecular weight showed higher viscosity and stronger crosslinking network(HRP-C,HRP-W and HRP-U),which exhibited stronger bile acid binding capacity.The present findings provide scientific evidence in the preparation technology of sea buckthorn polysaccharides with good antioxidant and bile acid binding capacity which are related to the structure affected by the extraction methods.展开更多
Aim: To evaluate the clinical efficacy of the traditional Chinese medicine sea buckthorn (SBT) in the treatment of patients with nonalcoholic fatty liver disease (NAFLD). Method: 94 patients with NAFLD were randomly d...Aim: To evaluate the clinical efficacy of the traditional Chinese medicine sea buckthorn (SBT) in the treatment of patients with nonalcoholic fatty liver disease (NAFLD). Method: 94 patients with NAFLD were randomly divided into two groups: 48 cases of patients received oral sea buckthorn 1.5 g (3 times a day) for three months as the treated group, and 46 cases received only the vehicle for three months as the control group. Serum lipids, transaminase and serum liver fibrosis indices were assessed at baseline and after SBT treatment. All patients underwent liver CT and Fibroscan examination at baseline and after treatment. Results: SBT treatment resulted in a significant decrease in the serum levels of alanine aminotransferase (ALT), LDL-C, hyaluronic acid and collagen type IV. The liver stiffness measurement (LSM) of the treated patients was significantly lower than that in the control or baseline. The CT liver/spleen ratio of the treated patients was also significantly increased. Conclusion: The results of our study demonstrated the beneficial effects of SBT on serum lipids, transaminase, and liver/spleen ratio and liver stiffness in patients with NAFLD, which may be further developed as a promising therapy for the treatment of NAFLD.展开更多
Background:Many natural polysaccharides have been reported to protect against skin aging through antioxidant pathways,but sea buckthorn polysaccharides have been less well studied in terms of antioxidant activity,and ...Background:Many natural polysaccharides have been reported to protect against skin aging through antioxidant pathways,but sea buckthorn polysaccharides have been less well studied in terms of antioxidant activity,and the related studies are not well developed.Objective:By examining the chemical structure of sea buckthorn polysaccharides,this research aimed to offer a scientific foundation for their use in cosmetics and explore the in vitro antioxidant potential of these polysaccharides.Materials:and Methods:We made SP(sea buckthorn crude polysaccharide)using both hot water extraction and ultrasonic extraction.Then,we used the anion exchange method to separate and purify it to get three different polysaccharides:SPA(polysaccharide eluted from deionized water),which is a neutral polysaccharide,and SPB(polysaccharides eluted from 0.1 M NaCl)and SPC(polysaccharide eluted from 0.2 M NaCl),which are acidic polysaccharides.The characteristics of the four polysaccharides(SP,SPA,SPB and SPC)were determined using high-performance gel permeation chromatography(HPGPC),high-performance liquid chromatography(HPLC),Fourier transform infrared spectroscopy(FTIR),scanning electron microscopy(SEM),thermogravimetric analysis(TGA),and x-ray diffractometry(XRD).In vitro antioxidant assays were used to test their antioxidant activities.Results:The in vitro antioxidant assay showed that the four sea buckthorn polysaccharides(SP,SPA,SPB,and SPC)were good at getting rid of superoxide anion and DPPH radicals,especially ABTS radicals.They were also better at getting rid of free radicals than VC at low concentrations,and they were also good at reducing other molecules.Conclusion:These results showed that sea buckthorn polysaccharide has certain antioxidant activity;therefore,sea buckthorn polysaccharide has a strong potential to be applied in antioxidant and anti-aging functional cosmetics.展开更多
In the last five years, the height increment and rhythm of growth, phenophase for the seedling phase and the character of resistence to adversity in forestation had been observed and compared. And variation of provena...In the last five years, the height increment and rhythm of growth, phenophase for the seedling phase and the character of resistence to adversity in forestation had been observed and compared. And variation of provenance of sca-buckthorn on geography was found out. The suitable provenance of sca-buckthorn wsa selected for experiment area.展开更多
Sea buckthorn market floated uncertainly within a narrow range. The market situation provided upward pressure on prices, and producer and consumer interest were poor, coupled with weak prices in the regional markets. ...Sea buckthorn market floated uncertainly within a narrow range. The market situation provided upward pressure on prices, and producer and consumer interest were poor, coupled with weak prices in the regional markets. The objectives of the study are: 1) to estimate the relationship between wild Sea buckthorn (SB) price and Supply, Demand, while some other factors of crude oil price and exchange rate by using simultaneous Supply-Demand and Price system equation and Vector Error Correction Method (VECM);2) to forecast the short-term and long-term SB price;3) to compare and evaluate the price forecasting models. Firstly, the data was analyzed by Ferris and Engle-Granger’s procedure;secondly, both price forecasting methodologies were tested by Pindyck-Rubinfeld and Makridakis’s procedure. The result shows that the VECM model is more efficient using yearly data;a short-term price forecast decreases, and a long-term price forecast is predicted to increase the Mongolian Sea buckthorn market.展开更多
Objective: This study is designed to observe the chronic toxicity after the administration of mulberry sea-buckthorn beverage concentrate for 3 months on rats and to predict the possible adverse effect and the potenti...Objective: This study is designed to observe the chronic toxicity after the administration of mulberry sea-buckthorn beverage concentrate for 3 months on rats and to predict the possible adverse effect and the potential toxicity target organs. Method: The rats (SPF level) were randomly divided into high-dose (20 mL/kg BW), middle-dose (10 mL/kg BW), low-dose (5 mL/kg BW) groups and negative control group (20 mL/kg BW of purified water) with 30 rats in each group. Each group was orally given mulberry sea-buckthorn beverage concentrate for 3 months and recovered by stop feeding samples for 2 weeks for a recovery observation. The rats’ general condition, the organ coefficient, the indexes of hematology and blood biochemistry and the histological changes of the main organs were determined. Result: The appearance and behavior of activity in rats showed no anomalies in all these groups and all the rats put on weight during this period. Comparing to the negative control group, no obvious differences were observed in the weekly weight and organ coefficient of each dose group. After 3 months of administration, HGB in both mulberry sea-buckthorn beverage concentrate low-dose group and high-dose group were increased. No significant differences were observed in the indexes of hematology after 2 weeks of recovery. CREA in low-dose, middle-dose and high-dose groups were significantly increased after 3 months of administration and it remained in the high level in middle-dose and high-dose group even after 2 weeks of recovery. No drug-related lesions were observed in the histological changes of major organs. Conclusion: The results show that long term use of mulberry concentrated sea-buckthorn beverage can lead to increased CREA, which suggested kidney toxicity. Although no obvious pathological change was found in kidney, we should pay attention to chronic kidney damage in the further research.展开更多
Skin diseases such as psoriasis, atopic dermatitis, burns, and other barrier insults pose ongoing therapeutic challenges, often requiring treatments that address both inflammation and skin barrier repair. The primary ...Skin diseases such as psoriasis, atopic dermatitis, burns, and other barrier insults pose ongoing therapeutic challenges, often requiring treatments that address both inflammation and skin barrier repair. The primary aim of this review is to provide a comprehensive overview of the potential benefits of sea buckthorn oil, derived from Hippophae rhamnoides, as a treatment for various skin diseases, detailing its anti-inflammatory properties, reporting benefits across several skin conditions, and highlighting challenges in formulation as well as preservation of the oil’s nutritional benefits. Drawing from a wide array of existing literature, this review investigates sea buckthorn oil’s rich composition of omega fatty acids, vitamins, and antioxidants, which may contribute to its therapeutic effects by modulating inflammatory pathways and enhancing skin barrier function. Clinical trials and patient case studies indicate that sea buckthorn oil shows promise in reducing erythema, inflammation, and improving skin sensitivity. However, challenges such as formulation stability, sourcing of quality oil, and variability in response to treatment across conditions need to be addressed. Future research should focus on optimizing formulations and conducting large-scale clinical trials to validate the therapeutic efficacy of sea buckthorn oil in the management of skin diseases. Additionally, further exploration of the regulatory mechanisms underlying its ability to modulate inflammation and repair the skin barrier is needed to fully elucidate its clinical potential. Sea buckthorn oil offers a novel and promising adjunctive treatment for psoriasis, atopic dermatitis, and wound-healing with significant potential to enhance therapeutic options for additional skin diseases.展开更多
Fermented dairy products are significant in the market due to their high nutritional value and unique flavor.However,they have more opportunities for improvement to meet the urgent demand for functional dairy products...Fermented dairy products are significant in the market due to their high nutritional value and unique flavor.However,they have more opportunities for improvement to meet the urgent demand for functional dairy products.as a novel prebiotic,sea buckthorn is widely used as a beneficial ingredient.in this review,we not only discuss its positive effects on the gut microbiota but also clarify its antioxidant and anti-inflammatory properties.Significantly,the unique benefits of sea buckthorn in enhancing the nutritional value of fermented dairy products,improving their physical properties,and enhancing the vitality of probiotics are also demonstrated.Furthermore,we point out the current limitations of sea buckthorn and recommend further research into sea buckthorn bioactive components to stimulate the production of functional dairy products.展开更多
Sea buckthorn oil(SBO)is rich in unsaturated fatty acids and various bioactive substances,and sea buckthorn leaf extract(SBLE)presents excellent antioxidant properties.In this study,SBO microcapsules containing SBLE w...Sea buckthorn oil(SBO)is rich in unsaturated fatty acids and various bioactive substances,and sea buckthorn leaf extract(SBLE)presents excellent antioxidant properties.In this study,SBO microcapsules containing SBLE were prepared by spray drying.Encapsulation and the addition of SBLE significantly improved the stability of SBO by measuring the oxidative induction time(OIT)and thermogravimetric analysis.The samples with 2%SBLE addition has the OIT value of 3.95 h,which was 2.8-fold of free oil.Encapsulation and SBLE addition both showed positive effects on keeping the oxidative stability under accelerated oxidation condition by comparing the color,peroxide vale,and MDA contents.In addition,SBLE significantly increased the antioxidant activity of encapsulated SBO.The effect of 2.0%SBLE was significantly better than 0.5%and 1.0%.Comparatively,SBLE was more effective than synthetic antioxidants BHA and BHT in increasing SBO stability and antioxidant properties.Overall,as a byproduct,SBLE has the potential as a natural additive to reduce the SBO degradation and play a synergistic role on human health.展开更多
Sea buckthorn has been proved to be a good substrate for lactic acid bacteria(LAB),but the research on the fermentation of sea buckthorn juice(SBJ)by LAB is still rare.This study was designed to determine the performa...Sea buckthorn has been proved to be a good substrate for lactic acid bacteria(LAB),but the research on the fermentation of sea buckthorn juice(SBJ)by LAB is still rare.This study was designed to determine the performance of fermented SBJ(FSBJ)in four LAB and investigate the sensory aspects of FSBJ.The results showed that the utilization rates of reducing sugar,titratable acid,and malic acid of SBJ fermented by Lactobacillus casei(SBJLc)were the highest and that lactic acid content was the greatest.The organic acid compositions of the four kinds of FSBJs were also different.The viable counts of all FSBJs was greater than 7 log CFU/mL,and that the viable count of SBJ fermented by SBJLc was the highest.In this study,the Electronic tongue combined with Electronic nose was used for the first time to study SBJ.Results showed that compared with the control,the response value of Sourness was significantly increased,while Bitterness and Astringency were significantly decreased.There was a significant difference in four FSBJs.The results of the electronic nose showed that SBJ fermented by Lactobacillus plantarum LP56(SBJLp)could be well identified by sensors sensitive to aromatic substances.The volatile compound contents before and after fermentation were significantly different,the highest being SBJLp.Finally,the results of sensory evaluation experiments showed that SBJLp was the most acceptable to consumers in terms of sensory evaluation.展开更多
基金supported by the National Natural Science Foundation of China(31201416)Programme of Introducing Talents of Discipline to Universities(B17018)。
文摘Hyperlipidemia constitutes a critical factor in the pathogenesis of vascular disease and gut microbiota dysbiosis.This study aims to investigate the role of sea buckthorn fruit oil(SBFO)in ameliorating hyperlipidemia,modulating gut microbiota,and elucidating the underlying mechanisms of its lipid-lowering effects.SBFO intervention in hyperlipidemic mice significantly reduced body weight and visceral adiposity index while mitigating symptoms associated with hyperlipidemia,including dyslipidemia,infla mmation,liver damage,and endothelial dysfunction.Real-time quantitative PCR and Western blot analyses revealed that SBFO enhanced the response of free fatty acid oxidation and breakdown(adenosine 5'-monophosphateactivated protein kinaseα,carnitine palmitoyltransferase1a,peroxisome proliferator-activated receptorα,and peroxisome proliferator-activated receptorγ)while suppressing the synthesis of triglycerides and fatty acids(sterol-regulatory element binding protein 1,fatty acid synthase,and acetyl CoA carboxylase)at the gene and protein expression levels.Moreover,SBFO intervention enriched the gut microbiota of hyperlipidemic mice by increasing the relative abundances of Lactobacillus,Faecalibaculum,and Allobaculum,while decreasing the Firmicutes/Bacteroidetes ratio.These findings suggest that SBFO exhibits promising therapeutic potential in attenuating hyperlipidemia in mice through the regulation of lipid metabolism and modulation of gut microbiota.
基金supported by the National Natural Science Foundation of China(32201994)。
文摘Sea buckthorn(Hippophae rhamnoides L.)is a natural homologous substance of medicine and food.Polysaccharide,as one of its primary active components,has very superior biological activity and can be used as a dietary supplement for functional foods,with good commercial prospects.Although initial progress has been made in the study of sea buckthorn polysaccharides,related studies have been fragmented and lacked systematic and generalization.This manuscript presents a critical analysis and systematic summary of the extraction and purification methods,structural characterization and physicochemical properties,biological activity and potential mechanisms,and structure-activity relationships of sea buckthorn polysaccharides.Accumulating evidence has indicated that sea buckthorn polysaccharides,which were widely prepared by water extraction and column chromatography purifications,exhibited exhibit superior biological activities in vitro and in vivo,including antioxidant,immunomodulatory,anti-inflammatory,hepatorenal protective,antibacterial,antiviral,and prebiotic activities.After analysis,it was concluded that there is a correlation between the relevant activities of sea buckthorn polysaccharides and that the structure of sea buckthorn polysaccharides has a great influence on their biological activity.We reviewed the challenges and limitations of sea buckthorn polysaccharides,summarized the critical aspects,and provided suggestions for potential breakthroughs in the research and application of sea buckthorn polysaccharide.
基金supported by 2024 Liaoning Province Graduate Education Teaching Reform Research Project(LNYJG2024251).
文摘The aim of this study was to explore the mechanism of action of sea buckthorn polyphenols in the treatment of hyperlipidemia through network pharmacology and molecular docking.The TCMSP pharmacology database was used to screen the polyphenols present in sea buckthorn,and then the SwissTargetPrediction and Uniprot databases were used to obtain the potential targets of sea buckthorn polyphenols,which were supplemented by the literature.In total,7 polyphenols and 154 potential targets were obtained.Through GeneCards,OMIM database,1358 hyperlipidemia-related targets were collected.We found that there were 101 targets at the intersection of components and diseases.Through GO and KEGG enrichment analysis,27 core targets were obtained,which were AKT1,TNF,TP53,IL-6,etc.in order of degree value.174 pathways were obtained from KEGG enrichment analysis,including AGE-RAGE signaling pathway in diabetic complications,fl uid shear stress and atherosclerosis,lipid and atherosclerosis,etc.The molecular docking of the main components to the targets was performed using OpenBabelGUI,AutoDockTools-1.5.6 software.Finally,the results were visualized using Cytoscape 3.9.1 software.The molecular docking results showed that sea buckthorn polyphenols have good binding ability with the key targets.Among them,such as quercetin and kaempferol,have good binding ability with TNF,TP53 and IL-6.For example,TNF binds to quercetin with a binding energy of-5.34 kcal/mol and to kaempferol with a binding energy of-6.22 kcal/mol;TP53 binds to kaempferol with a binding energy of-5.32 kcal/mol;IL-6 binds to quercetin with a binding energy of-5.62 kcal/mol,etc.Therefore,the network pharmacology study showed that the treatment of hyperlipidemia by sea buckthorn polyphenols can be realized by multi-component-multi-target-multi-pathway together,which provides some reference for the later study of sea buckthorn polyphenols in the treatment of hyperlipidemia.
基金supported by Biological Manufacturing Technology of natural active Products(2021YFC2101500)2021.07-2024.06 National Key Research and Development Program 125.5 of the Ministry of Science and Technology.
文摘Background:Seabuckthorn is a widely distributed,high-yielding shrub valued for its medicinal and nutritional properties,with its fruit rich in flavonoids and polysaccharides.While studies have focused on the anti-inflammatory,antioxidant,anticancer,and immune-boosting effects of these compounds,research on their skincare benefits is limited.Additionally,there is a lack of method development and characterization of their actual skincare efficacy.Objective:The main objective of this study was to investigate the antimicrobial,whitening,moisturizing,and restorative effects of seabuckthorn polysaccharides and flavonoids.An innovative gel leave-on mask was developed with these active ingredients,and its stability and skincare efficacy were evaluated through characterization experiments focusing on antibacterial,whitening,moisturizing,and restorative effects.Materials and Methods:Seabuckthorn polysaccharides and flavonoids were extracted using both aqueous and alcoholic methods.The antibacterial,antioxidant and whitening efficacy of sea buckthorn flavonoids was investigated by the antibacterial test of Propionibacterium acnes,ABTS free radical scavenging test and tyrosinase inhibition test.An innovative gel leave-on mask formula was developed,incorporating glycerol glucoside and hydrolyzed collagen for added moisturizing and reparative benefits.The stability of the gel mask was tested for pH stability,ionic strength stability,and thermodynamic stability using measurements of particle size,polydispersity index(PDI),and zeta potential.Finally,the practical efficacy of the gel leave-on mask was evaluated through participant trials,measuring skin parameters such as vascular density,skin roughness,moisture content,and pigmentation.Results:The study demonstrated that seabuckthorn flavonoids exhibited excellent antimicrobial,anti-inflammatory and whitening effects.The gel leave-on mask formulation proved stable.Participants showed significant improvements in skin parameters,including reduced roughness,increased moisture content,and enhanced pigmentation balance,confirming the practical efficacy of the product.Conclusion:In conclusion,this study fills the gap in the systematic verification of the multifaceted skincare benefits of seabuckthorn flavonoids and polysaccharides,while also developing a product that efficiently integrates these two active ingredients.The formulation's practical efficacy was confirmed,showing significant effects in antibacterial,anti-inflammatory,and whitening applications.
基金The Guangdong Basic and Applied Basic Research Foundation(2022A1515010730)National Natural Science Foundation of China(32001647)+2 种基金National Natural Science Foundation of China(31972022)Financial and moral assistance supported by the Guangdong Basic and Applied Basic Research Foundation(2019A1515011996)111 Project(B17018)。
文摘In this study,the structural characters,antioxidant activities and bile acid-binding ability of sea buckthorn polysaccharides(HRPs)obtained by the commonly used hot water(HRP-W),pressurized hot water(HRP-H),ultrasonic(HRP-U),acid(HRP-C)and alkali(HRP-A)assisted extraction methods were investigated.The results demonstrated that extraction methods had significant effects on extraction yield,monosaccharide composition,molecular weight,particle size,triple-helical structure,and surface morphology of HRPs except for the major linkage bands.Thermogravimetric analysis showed that HRP-U with filamentous reticular microstructure exhibited better thermal stability.The HRP-A with the lowest molecular weight and highest arabinose content possessed the best antioxidant activities.Moreover,the rheological analysis indicated that HRPs with higher galacturonic acid content and molecular weight showed higher viscosity and stronger crosslinking network(HRP-C,HRP-W and HRP-U),which exhibited stronger bile acid binding capacity.The present findings provide scientific evidence in the preparation technology of sea buckthorn polysaccharides with good antioxidant and bile acid binding capacity which are related to the structure affected by the extraction methods.
文摘Aim: To evaluate the clinical efficacy of the traditional Chinese medicine sea buckthorn (SBT) in the treatment of patients with nonalcoholic fatty liver disease (NAFLD). Method: 94 patients with NAFLD were randomly divided into two groups: 48 cases of patients received oral sea buckthorn 1.5 g (3 times a day) for three months as the treated group, and 46 cases received only the vehicle for three months as the control group. Serum lipids, transaminase and serum liver fibrosis indices were assessed at baseline and after SBT treatment. All patients underwent liver CT and Fibroscan examination at baseline and after treatment. Results: SBT treatment resulted in a significant decrease in the serum levels of alanine aminotransferase (ALT), LDL-C, hyaluronic acid and collagen type IV. The liver stiffness measurement (LSM) of the treated patients was significantly lower than that in the control or baseline. The CT liver/spleen ratio of the treated patients was also significantly increased. Conclusion: The results of our study demonstrated the beneficial effects of SBT on serum lipids, transaminase, and liver/spleen ratio and liver stiffness in patients with NAFLD, which may be further developed as a promising therapy for the treatment of NAFLD.
基金the“JUN-MA”High-level Talents Program of Inner Mongolia University(No.21300–5195112,No.21300–5205107)the funding from the Science&Technology Department of Inner Mongolia Autonomous Region(No.2021CG0029,No.15000021T000000020229)the funding from the Agriculture&Animal Husbandry Department of Inner Mongolia Autonomous Region(No.21300–5223323).
文摘Background:Many natural polysaccharides have been reported to protect against skin aging through antioxidant pathways,but sea buckthorn polysaccharides have been less well studied in terms of antioxidant activity,and the related studies are not well developed.Objective:By examining the chemical structure of sea buckthorn polysaccharides,this research aimed to offer a scientific foundation for their use in cosmetics and explore the in vitro antioxidant potential of these polysaccharides.Materials:and Methods:We made SP(sea buckthorn crude polysaccharide)using both hot water extraction and ultrasonic extraction.Then,we used the anion exchange method to separate and purify it to get three different polysaccharides:SPA(polysaccharide eluted from deionized water),which is a neutral polysaccharide,and SPB(polysaccharides eluted from 0.1 M NaCl)and SPC(polysaccharide eluted from 0.2 M NaCl),which are acidic polysaccharides.The characteristics of the four polysaccharides(SP,SPA,SPB and SPC)were determined using high-performance gel permeation chromatography(HPGPC),high-performance liquid chromatography(HPLC),Fourier transform infrared spectroscopy(FTIR),scanning electron microscopy(SEM),thermogravimetric analysis(TGA),and x-ray diffractometry(XRD).In vitro antioxidant assays were used to test their antioxidant activities.Results:The in vitro antioxidant assay showed that the four sea buckthorn polysaccharides(SP,SPA,SPB,and SPC)were good at getting rid of superoxide anion and DPPH radicals,especially ABTS radicals.They were also better at getting rid of free radicals than VC at low concentrations,and they were also good at reducing other molecules.Conclusion:These results showed that sea buckthorn polysaccharide has certain antioxidant activity;therefore,sea buckthorn polysaccharide has a strong potential to be applied in antioxidant and anti-aging functional cosmetics.
文摘In the last five years, the height increment and rhythm of growth, phenophase for the seedling phase and the character of resistence to adversity in forestation had been observed and compared. And variation of provenance of sca-buckthorn on geography was found out. The suitable provenance of sca-buckthorn wsa selected for experiment area.
文摘Sea buckthorn market floated uncertainly within a narrow range. The market situation provided upward pressure on prices, and producer and consumer interest were poor, coupled with weak prices in the regional markets. The objectives of the study are: 1) to estimate the relationship between wild Sea buckthorn (SB) price and Supply, Demand, while some other factors of crude oil price and exchange rate by using simultaneous Supply-Demand and Price system equation and Vector Error Correction Method (VECM);2) to forecast the short-term and long-term SB price;3) to compare and evaluate the price forecasting models. Firstly, the data was analyzed by Ferris and Engle-Granger’s procedure;secondly, both price forecasting methodologies were tested by Pindyck-Rubinfeld and Makridakis’s procedure. The result shows that the VECM model is more efficient using yearly data;a short-term price forecast decreases, and a long-term price forecast is predicted to increase the Mongolian Sea buckthorn market.
文摘Objective: This study is designed to observe the chronic toxicity after the administration of mulberry sea-buckthorn beverage concentrate for 3 months on rats and to predict the possible adverse effect and the potential toxicity target organs. Method: The rats (SPF level) were randomly divided into high-dose (20 mL/kg BW), middle-dose (10 mL/kg BW), low-dose (5 mL/kg BW) groups and negative control group (20 mL/kg BW of purified water) with 30 rats in each group. Each group was orally given mulberry sea-buckthorn beverage concentrate for 3 months and recovered by stop feeding samples for 2 weeks for a recovery observation. The rats’ general condition, the organ coefficient, the indexes of hematology and blood biochemistry and the histological changes of the main organs were determined. Result: The appearance and behavior of activity in rats showed no anomalies in all these groups and all the rats put on weight during this period. Comparing to the negative control group, no obvious differences were observed in the weekly weight and organ coefficient of each dose group. After 3 months of administration, HGB in both mulberry sea-buckthorn beverage concentrate low-dose group and high-dose group were increased. No significant differences were observed in the indexes of hematology after 2 weeks of recovery. CREA in low-dose, middle-dose and high-dose groups were significantly increased after 3 months of administration and it remained in the high level in middle-dose and high-dose group even after 2 weeks of recovery. No drug-related lesions were observed in the histological changes of major organs. Conclusion: The results show that long term use of mulberry concentrated sea-buckthorn beverage can lead to increased CREA, which suggested kidney toxicity. Although no obvious pathological change was found in kidney, we should pay attention to chronic kidney damage in the further research.
文摘Skin diseases such as psoriasis, atopic dermatitis, burns, and other barrier insults pose ongoing therapeutic challenges, often requiring treatments that address both inflammation and skin barrier repair. The primary aim of this review is to provide a comprehensive overview of the potential benefits of sea buckthorn oil, derived from Hippophae rhamnoides, as a treatment for various skin diseases, detailing its anti-inflammatory properties, reporting benefits across several skin conditions, and highlighting challenges in formulation as well as preservation of the oil’s nutritional benefits. Drawing from a wide array of existing literature, this review investigates sea buckthorn oil’s rich composition of omega fatty acids, vitamins, and antioxidants, which may contribute to its therapeutic effects by modulating inflammatory pathways and enhancing skin barrier function. Clinical trials and patient case studies indicate that sea buckthorn oil shows promise in reducing erythema, inflammation, and improving skin sensitivity. However, challenges such as formulation stability, sourcing of quality oil, and variability in response to treatment across conditions need to be addressed. Future research should focus on optimizing formulations and conducting large-scale clinical trials to validate the therapeutic efficacy of sea buckthorn oil in the management of skin diseases. Additionally, further exploration of the regulatory mechanisms underlying its ability to modulate inflammation and repair the skin barrier is needed to fully elucidate its clinical potential. Sea buckthorn oil offers a novel and promising adjunctive treatment for psoriasis, atopic dermatitis, and wound-healing with significant potential to enhance therapeutic options for additional skin diseases.
基金supported by the Liaoning revitalization talents Program(XLYC2213026,XLYC2402005)Liaoning Province Science and technology Plan Project(2024JH2/101900005)+1 种基金introduction of talents(High-Level)research Start-up Fund Project of Shenyang agricultural University(2023YJrc002)Shenyang Science and Technology Innovation Platform Project(21-103-0-14,21-104-0-28).
文摘Fermented dairy products are significant in the market due to their high nutritional value and unique flavor.However,they have more opportunities for improvement to meet the urgent demand for functional dairy products.as a novel prebiotic,sea buckthorn is widely used as a beneficial ingredient.in this review,we not only discuss its positive effects on the gut microbiota but also clarify its antioxidant and anti-inflammatory properties.Significantly,the unique benefits of sea buckthorn in enhancing the nutritional value of fermented dairy products,improving their physical properties,and enhancing the vitality of probiotics are also demonstrated.Furthermore,we point out the current limitations of sea buckthorn and recommend further research into sea buckthorn bioactive components to stimulate the production of functional dairy products.
基金supported by Shaanxi high-level talent special support plan(TZ0389)Shaanxi Province Key R&D Program(2022NY-021).
文摘Sea buckthorn oil(SBO)is rich in unsaturated fatty acids and various bioactive substances,and sea buckthorn leaf extract(SBLE)presents excellent antioxidant properties.In this study,SBO microcapsules containing SBLE were prepared by spray drying.Encapsulation and the addition of SBLE significantly improved the stability of SBO by measuring the oxidative induction time(OIT)and thermogravimetric analysis.The samples with 2%SBLE addition has the OIT value of 3.95 h,which was 2.8-fold of free oil.Encapsulation and SBLE addition both showed positive effects on keeping the oxidative stability under accelerated oxidation condition by comparing the color,peroxide vale,and MDA contents.In addition,SBLE significantly increased the antioxidant activity of encapsulated SBO.The effect of 2.0%SBLE was significantly better than 0.5%and 1.0%.Comparatively,SBLE was more effective than synthetic antioxidants BHA and BHT in increasing SBO stability and antioxidant properties.Overall,as a byproduct,SBLE has the potential as a natural additive to reduce the SBO degradation and play a synergistic role on human health.
基金This research was supported by Xinjiang Production and Construction Corps Innovation Team Building Projects in Key Areas(2017CB012)Projects of Innovation and Development Pillar Program for Key Industries in Southern Xinjiang of Xinjiang Production and Construction Corps(2018DB002)Young and Middle-aged Leading Talent Project(2020CB025).
文摘Sea buckthorn has been proved to be a good substrate for lactic acid bacteria(LAB),but the research on the fermentation of sea buckthorn juice(SBJ)by LAB is still rare.This study was designed to determine the performance of fermented SBJ(FSBJ)in four LAB and investigate the sensory aspects of FSBJ.The results showed that the utilization rates of reducing sugar,titratable acid,and malic acid of SBJ fermented by Lactobacillus casei(SBJLc)were the highest and that lactic acid content was the greatest.The organic acid compositions of the four kinds of FSBJs were also different.The viable counts of all FSBJs was greater than 7 log CFU/mL,and that the viable count of SBJ fermented by SBJLc was the highest.In this study,the Electronic tongue combined with Electronic nose was used for the first time to study SBJ.Results showed that compared with the control,the response value of Sourness was significantly increased,while Bitterness and Astringency were significantly decreased.There was a significant difference in four FSBJs.The results of the electronic nose showed that SBJ fermented by Lactobacillus plantarum LP56(SBJLp)could be well identified by sensors sensitive to aromatic substances.The volatile compound contents before and after fermentation were significantly different,the highest being SBJLp.Finally,the results of sensory evaluation experiments showed that SBJLp was the most acceptable to consumers in terms of sensory evaluation.
