辣椒红色素是目前全球销量最大的纯天然可食用色素,培育高辣椒红色素品种为辣椒产业重要任务。通过对255份一年生栽培种辣椒核心种质的辣椒红素含量进行全基因组关联分析(genome-wide association study,GWAS),在第1、2、3、5、6、8、9...辣椒红色素是目前全球销量最大的纯天然可食用色素,培育高辣椒红色素品种为辣椒产业重要任务。通过对255份一年生栽培种辣椒核心种质的辣椒红素含量进行全基因组关联分析(genome-wide association study,GWAS),在第1、2、3、5、6、8、9、10、11和12号染色体均关联到与辣椒红素含量显著相关的区间,关联区间内共包括93个基因,根据功能注释和转录表达数据预测了3个影响辣椒果实中辣椒红素含量的候选基因。通过对高辣椒红色素材料Pep-340、低辣椒红色素材料Pep-276构建的F2群体进行混合分组分析法-测序(bulked segregant analysis-sequencing,BSA-seq)分析,在第1、3、5和10号染色体定位到与辣椒红色素含量相关区间,其中第3和5号染色体上的定位区间与GWAS分析中的显著相关区间相近或重合;利用这两个区间的In Del分子标记,进行遗传连锁分析,将调控辣椒红色素含量基因定位在3号染色体的q CC3.1,物理位置为22.8~25.9 Mb,其中含有99个基因,根据功能注释和转录组分析,预测了4个影响辣椒果实中辣椒红色素含量的候选基因Capana03g001314、Capana03g001325、Capana03g001334和Capana03g001387。研究结果为调控辣椒中辣椒红色素含量基因精细定位及分子标记辅助选择育种奠定基础。展开更多
The color difference of capsicum fruit is closely related to the type and content of pigment in the peel,which is mainly determined by anthocyanins,chlorophyll,and carotenoids.This study used green“CA59”and purple“...The color difference of capsicum fruit is closely related to the type and content of pigment in the peel,which is mainly determined by anthocyanins,chlorophyll,and carotenoids.This study used green“CA59”and purple“Z81”pepper fruits as parents to create the F2 generation.The fruit color of 466 F2 population was identified,and the extreme individuals from this population were selected for Bulked Segregant Analysis(BSA)using resequencing.Genetic analysis revealed that a pair of genes controls the expression of the purple fruit trait in capsicum.Using functional annotation,expression analysis,and sequencing analysis of candidate genes,it was determined that there were four genes in the region between InDel 67 and InDel 75(185,664,068 BP-186,514,350 bp)on chromosome 10,that is the linkage interval for pepper purple fruit.There are 7 SNPs in the CaMYB1 gene(Capann_59V1aChr10g016200)in the pepper variety“Z81”.Of these,4 SNPs are located in the gene’s coding region.These 4 SNPs lead to 2 mutations that do not change the amino acid sequence(synonymous mutations)and 2 mutations that do change the amino acid sequence(non-synonymous mutations).Additionally,the expression level of the CaMYB1 gene in the purple fruit of“Z81”is significantly higher than that in the green fruit of“CA59”.CaMYB1 is believed to be a crucial candidate gene in regulating anthocyanin production in purple capsicum fruit.A molecular marker,InDel 67,was successfully developed,with a total separation rate of 92.4%.展开更多
基金supported by the National Natural Science Foundation of China(U2IA20230)the Guangdong Modern Vegetable Industry Technology System Project(2024CXTD08)+2 种基金the Guangdong Basic and Applied Basic Research Foundation(2024A1515010403)the Xizang Autonomous Region of Lhasa City Science and Technology Project(LSKJ202418)the Guangdong Provincial Department of Agriculture and Rural Affairs(Selection and Breeding of New High-Yielding and High-Quality Pepper Varieties)(2024-NPY-00-020).
文摘The color difference of capsicum fruit is closely related to the type and content of pigment in the peel,which is mainly determined by anthocyanins,chlorophyll,and carotenoids.This study used green“CA59”and purple“Z81”pepper fruits as parents to create the F2 generation.The fruit color of 466 F2 population was identified,and the extreme individuals from this population were selected for Bulked Segregant Analysis(BSA)using resequencing.Genetic analysis revealed that a pair of genes controls the expression of the purple fruit trait in capsicum.Using functional annotation,expression analysis,and sequencing analysis of candidate genes,it was determined that there were four genes in the region between InDel 67 and InDel 75(185,664,068 BP-186,514,350 bp)on chromosome 10,that is the linkage interval for pepper purple fruit.There are 7 SNPs in the CaMYB1 gene(Capann_59V1aChr10g016200)in the pepper variety“Z81”.Of these,4 SNPs are located in the gene’s coding region.These 4 SNPs lead to 2 mutations that do not change the amino acid sequence(synonymous mutations)and 2 mutations that do change the amino acid sequence(non-synonymous mutations).Additionally,the expression level of the CaMYB1 gene in the purple fruit of“Z81”is significantly higher than that in the green fruit of“CA59”.CaMYB1 is believed to be a crucial candidate gene in regulating anthocyanin production in purple capsicum fruit.A molecular marker,InDel 67,was successfully developed,with a total separation rate of 92.4%.
