In this work, the surface properties of novel sugar-containing polymers, α-allyl glucoside (AG)/acrylonitrile (AN)copolymers, were studied by contact angle, protein adsorption and cell adhesion measurements. It was f...In this work, the surface properties of novel sugar-containing polymers, α-allyl glucoside (AG)/acrylonitrile (AN)copolymers, were studied by contact angle, protein adsorption and cell adhesion measurements. It was found that the contactangle of the copolymer films decreased from 68° to 30° with the increase of AG content in the copolymer. The adsorptionamount of bovine serum albumin (BSA) and the adhesive macrophage onto the film surface also decreased significantly withincreasing α-allyl glucoside content from 0 to 42 wt% in the copolymer. These preliminary results reveal that both thehydrophilicity and the biocompatibility of polyacrylonitrile-based membranes could be improved by copolymerizin gacrylonitrile with vinyl carbohydrates.展开更多
Surface modification of microporous polypropylene hollow fiber membranes was performed by radical-induced graft polymerization of N,N-dimethylaminoethyl methacrylate (DMAEMA). The influences of temperature, monomer co...Surface modification of microporous polypropylene hollow fiber membranes was performed by radical-induced graft polymerization of N,N-dimethylaminoethyl methacrylate (DMAEMA). The influences of temperature, monomer concentration and pre-adsorbed amount of benzoyl peroxide on grafting degree were studied respectively. It was found that the appropriate graft temperature was 75 'C, at which the grafting degree was the highest and the hydrolytic decomposition of DMAEMA the lowest. Scanning electron photomicrography and the average pore diameters of the modified membranes demonstrated that part of the micropores on the membrane surface was plugged by the grafted polyDMAEMA chains, especially at high grafting degree. Contact angle and water swelling experiments showed that a moderate grafting degree could improve the hydrophilicity of the membranes. In the range of 11.3%-12.0% grafting degree, the water swelling percentage reached its maximum (51.1%) and the contact angle reached its minimum (74 degrees). The bovine serum albumin (BSA) adsorption experiment indicated that the grafted polyDMAEMA had a dual effect on protein adsorption. At the first stage, the BSA adsorption decreased with increasing of DMAEMA grafting degree. As the interaction between BSA and polyDMAEMA on membrane surface increased, the BSA adsorption increased with increasing of DMAEMA grafting degree.展开更多
An ideal porous scaffold for bone tissue engineering should exhibit a degradation rate that matches the regeneration rate of the host tissue,thereby facilitating complete tissue replacement.Magnesium(Mg) and its alloy...An ideal porous scaffold for bone tissue engineering should exhibit a degradation rate that matches the regeneration rate of the host tissue,thereby facilitating complete tissue replacement.Magnesium(Mg) and its alloys have emerged as promising biomaterials due to their excellent biocompatibility and favorable mechanical properties.However,conventional manufacturing techniques often fail to eliminate microscopic structural defects within the scaffold's pores,which can accelerate degradation and hinder clinical applications.In this study,electrochemical polishing(EP) was employed to optimize the surface of porous scaffolds by effectively eliminating surface defects.After immersion in Hanks' solution for 7 days,the degradation rate of the EP-treated scaffolds was reduced by 62.5%.To better simulate the influence of proteins on scaffold degradation,bovine serum albumin was added to the Hanks' solution.Under this simulated physiological environment,EP treatment led to a 41.7%reduction in the degradation rate.Furthermore,in vivo implantation experiments,EP treatment resulted in an 83.1% decrease in the degradation rate of the porous scaffolds.The optimal EP parameters were first determined,followed by a systematic investigation of the degradation behavior of both untreated and EP-treated porous scaffolds through experimental analysis and first-principles calculations.The findings provide new insights into the degradation regulation of Mg-based porous scaffolds and establish a solid scientific foundation for their future applications in bone tissue engineering.展开更多
In this paper, gas-assisted magnetic separation （GAMS）, a technique that combines magnetic separation with flotation, was investigated for the potential large-scale separation of proteins, The GAMS process includes ...In this paper, gas-assisted magnetic separation （GAMS）, a technique that combines magnetic separation with flotation, was investigated for the potential large-scale separation of proteins, The GAMS process includes adsorption of target proteins and magnetic separation to recover protein-loaded magnetic particles from the dilute biosuspension with the assistance of bubbles, Microsized ethylenediamine- functionalized poly（glycidyl methacrylate） superparamagnetic microspheres （MPMs） and bovine serum albumin （BSA） were used as a model system. The feasibility of GAMS for capturing BSA-loaded MPMs from an appropriate medium was shown, High recovery of BSA-loaded MPMs was obtained by simple adjustment of the initial solution pH without extra detergents and antifoaming agents. The GAMS con- ditions were consistent with the adsorption conditions, and no proteins were desorbed from the MPMs during this process. Under the optimal conditions, the separation rate and recovery percentage reached 410 mL/min and 98% in 0.61 min, respectively. Conformational changes of BSA during the GAMS process were investigated by fluorescence spectroscopy and circular dichroism spectrometry,展开更多
基金The authors are grateful to the National Natural Science Foundation of China for financial support(Grant No.20074033).
文摘In this work, the surface properties of novel sugar-containing polymers, α-allyl glucoside (AG)/acrylonitrile (AN)copolymers, were studied by contact angle, protein adsorption and cell adhesion measurements. It was found that the contactangle of the copolymer films decreased from 68° to 30° with the increase of AG content in the copolymer. The adsorptionamount of bovine serum albumin (BSA) and the adhesive macrophage onto the film surface also decreased significantly withincreasing α-allyl glucoside content from 0 to 42 wt% in the copolymer. These preliminary results reveal that both thehydrophilicity and the biocompatibility of polyacrylonitrile-based membranes could be improved by copolymerizin gacrylonitrile with vinyl carbohydrates.
基金The work was supported by the National Natural Science Foundation of China (Grant No. 20074033) and the High-TechResearch and Development Program of China (Grant no. 2002AA601230).
文摘Surface modification of microporous polypropylene hollow fiber membranes was performed by radical-induced graft polymerization of N,N-dimethylaminoethyl methacrylate (DMAEMA). The influences of temperature, monomer concentration and pre-adsorbed amount of benzoyl peroxide on grafting degree were studied respectively. It was found that the appropriate graft temperature was 75 'C, at which the grafting degree was the highest and the hydrolytic decomposition of DMAEMA the lowest. Scanning electron photomicrography and the average pore diameters of the modified membranes demonstrated that part of the micropores on the membrane surface was plugged by the grafted polyDMAEMA chains, especially at high grafting degree. Contact angle and water swelling experiments showed that a moderate grafting degree could improve the hydrophilicity of the membranes. In the range of 11.3%-12.0% grafting degree, the water swelling percentage reached its maximum (51.1%) and the contact angle reached its minimum (74 degrees). The bovine serum albumin (BSA) adsorption experiment indicated that the grafted polyDMAEMA had a dual effect on protein adsorption. At the first stage, the BSA adsorption decreased with increasing of DMAEMA grafting degree. As the interaction between BSA and polyDMAEMA on membrane surface increased, the BSA adsorption increased with increasing of DMAEMA grafting degree.
基金supported by the Project of Zhongyuan Critical Metals Laboratory(Nos.GJJSGFYQ202406 and GJJSGFYQ202318)the National Natural Science Foundation of China(Nos.51701184,51671175 and 52301024)+1 种基金the Young Backbone Teachers Foundation of Zhengzhou Universitythe Natural Science Foundation of Henan Province(No.232300421342)
文摘An ideal porous scaffold for bone tissue engineering should exhibit a degradation rate that matches the regeneration rate of the host tissue,thereby facilitating complete tissue replacement.Magnesium(Mg) and its alloys have emerged as promising biomaterials due to their excellent biocompatibility and favorable mechanical properties.However,conventional manufacturing techniques often fail to eliminate microscopic structural defects within the scaffold's pores,which can accelerate degradation and hinder clinical applications.In this study,electrochemical polishing(EP) was employed to optimize the surface of porous scaffolds by effectively eliminating surface defects.After immersion in Hanks' solution for 7 days,the degradation rate of the EP-treated scaffolds was reduced by 62.5%.To better simulate the influence of proteins on scaffold degradation,bovine serum albumin was added to the Hanks' solution.Under this simulated physiological environment,EP treatment led to a 41.7%reduction in the degradation rate.Furthermore,in vivo implantation experiments,EP treatment resulted in an 83.1% decrease in the degradation rate of the porous scaffolds.The optimal EP parameters were first determined,followed by a systematic investigation of the degradation behavior of both untreated and EP-treated porous scaffolds through experimental analysis and first-principles calculations.The findings provide new insights into the degradation regulation of Mg-based porous scaffolds and establish a solid scientific foundation for their future applications in bone tissue engineering.
文摘In this paper, gas-assisted magnetic separation （GAMS）, a technique that combines magnetic separation with flotation, was investigated for the potential large-scale separation of proteins, The GAMS process includes adsorption of target proteins and magnetic separation to recover protein-loaded magnetic particles from the dilute biosuspension with the assistance of bubbles, Microsized ethylenediamine- functionalized poly（glycidyl methacrylate） superparamagnetic microspheres （MPMs） and bovine serum albumin （BSA） were used as a model system. The feasibility of GAMS for capturing BSA-loaded MPMs from an appropriate medium was shown, High recovery of BSA-loaded MPMs was obtained by simple adjustment of the initial solution pH without extra detergents and antifoaming agents. The GAMS con- ditions were consistent with the adsorption conditions, and no proteins were desorbed from the MPMs during this process. Under the optimal conditions, the separation rate and recovery percentage reached 410 mL/min and 98% in 0.61 min, respectively. Conformational changes of BSA during the GAMS process were investigated by fluorescence spectroscopy and circular dichroism spectrometry,
