In real-world autonomous driving tests,unexpected events such as pedestrians or wild animals suddenly entering the driving path can occur.Conducting actual test drives under various weather conditions may also lead to...In real-world autonomous driving tests,unexpected events such as pedestrians or wild animals suddenly entering the driving path can occur.Conducting actual test drives under various weather conditions may also lead to dangerous situations.Furthermore,autonomous vehicles may operate abnormally in bad weather due to limitations of their sensors and GPS.Driving simulators,which replicate driving conditions nearly identical to those in the real world,can drastically reduce the time and cost required for market entry validation;consequently,they have become widely used.In this paper,we design a virtual driving test environment capable of collecting and verifying SiLS data under adverse weather conditions using multi-source images.The proposed method generates a virtual testing environment that incorporates various events,including weather,time of day,and moving objects,that cannot be easily verified in real-world autonomous driving tests.By setting up scenario-based virtual environment events,multi-source image analysis and verification using real-world DCUs(Data Concentrator Units)with V2X-Car edge cloud can effectively address risk factors that may arise in real-world situations.We tested and validated the proposed method with scenarios employing V2X communication and multi-source image analysis.展开更多
Human epidermal growth factor receptor 2(HER2) overexpression is increasingly recognized as a frequent molecular abnormality in gastric and gastroesophageal cancer. With the recent introduction of HER2 molecular targe...Human epidermal growth factor receptor 2(HER2) overexpression is increasingly recognized as a frequent molecular abnormality in gastric and gastroesophageal cancer. With the recent introduction of HER2 molecular targeted therapy for patients with advanced gastric cancer, determination of HER2 status is crucial in order to select patients who may benefit from this treatment. This paper provides an update on our knowledge of HER2 in gastric and gastroesophageal cancer, including the prognostic relevance of HER2, the key differences between HER2 protein expression interpretation in breast and gastric cancer, the detection methods and the immunohistochemistry scoring system.展开更多
China has pledged to peak carbon dioxide(CO_(2))emissions by 2030 and achieve carbon neutrality by 2060.Carbon capture and storage(CCS)will play a key role in these efforts.Over the past several years,the China Geolog...China has pledged to peak carbon dioxide(CO_(2))emissions by 2030 and achieve carbon neutrality by 2060.Carbon capture and storage(CCS)will play a key role in these efforts.Over the past several years,the China Geological Survey(CGS)has completed the Suitability Evaluation Map of CO_(2)Geological Storage in Main Sedimentary Basins in China and Adjacent Sea Regions in 2017.This map reflects the suitability of the first-and secondary-order tectonic units within sedimentary basins for cO_(2)geological storage for CCS planning.The Junggar Basin is recognized as an important region for future CCS projects.Results from a mesoscale evaluation using the volume method indicate that deep saline aquifers represent the most significant resources for CO_(2)storage,with potential ranging from 48×10^(9)to 164×10^(9)t(with a P50 value of 96×10^(9)t).The highest storage potential is identified in the central and northern parts of the basin,reaching up to 9.5×10^(6)t/km^(2)at the P50 probability level.In contrast,the hinterland,eastern,and western parts of the basin generally exhibit storage potential of below 1.0×10^(6)t/km^(2)at the same probability level.The CGs has also characterized historical CO_(2)plume migration in reservoirs at the storage site of the Shenhua CCS demonstration project and conducted numerical simulations of CO_(2)plume migration for periods of 10 and 20 years following the shutdown of the injection well.The CGS implemented a kiloton-scale pilot test on CO_(2)-enhanced water recovery(CO_(2)-EWR)in eastern Junggar,revealing that CO_(2)flooding can improve the pressure for fluid production,with the highest ratio of CO_(2)to produced fluids estimated at approximately 1.2.Besides,an observation field for natural CO_(2)leakage,covering about 930 m^(2),was built in Qinghai Province.In natural CO_(2)fields or at artificial CO_(2)injection research sites,cO_(2)leakage points are primarily related to the distribution of faults(especially fault crossing),which can serve as pathways for CO_(2)leakage.The observation field provides a natural analog to wellbore failure and offers an opportunity to further monitor CO_(2)geological storage sites.However,it has been inferred that borehole ZK10 at the observation field has become a leakage pathway due to the drilling activities,inadequate well-plugging,and abandonment procedures without considering CO_(2)corrosion.展开更多
The bulge test is a widely utilized method for assessing the mechanical properties of thin films,including metals,polymers,and semiconductors.However,as film thickness diminishes to nanometer scales,boundary condition...The bulge test is a widely utilized method for assessing the mechanical properties of thin films,including metals,polymers,and semiconductors.However,as film thickness diminishes to nanometer scales,boundary conditions dominated by weak van der Waals forces significantly impact mechanical responses.Instead of sample fracture,interfacial shear deformation and delamination become the primary deformation modes,thereby challenging the applicability of conventional bulge models.To accommodate the interfacial effect,a modified mechanical model based on the bulge test has been proposed.This review summarizes recent advancements in the bulge test to highlight the potential challenges and opportunities for future research.展开更多
Microstructural heterogeneity of low-permeability sandstone roofs of deep unmineable coal seams due to diagenesis significantly affects rock mechanical behavior,greatly impacting the sealing potential of in situ CO_(2...Microstructural heterogeneity of low-permeability sandstone roofs of deep unmineable coal seams due to diagenesis significantly affects rock mechanical behavior,greatly impacting the sealing potential of in situ CO_(2) sequestration and the structural stability of the geological formation.However,little is known about how the microstructure of different mineral groups influences the multiscale mechanical behavior of deep sandstone.This study proposes a new method for quantitatively characterizing the multiscale mechanical properties of low-permeability sandstone and shows the mechanisms responsible for mechanical failure at the micro-,meso-,and macroscale.Triaxial compression tests and targeted nanoindentation tests were conducted to assess the micro-and macroscale mechanical properties of different types of sandstone.The micro-and macroscale experiments were coupled with numerical simulations of compression using a unified cohesive model based on Voronoi polygons to clarify the multiscale mechanical behavior.The results indicate that quartz,the primary mineral component of the sandstones examined,exhibits the strongest micromechanical properties,followed by feldspar,calcite,and clay minerals.Compared to polycrystalline quartz,monocrystalline quartz has a more stable microstructure and is mechanically stronger.The macro-mechanical properties of tight sandstone samples are weakened by increased microstructural inhomogeneity and larger grain size.This leads to a higher likelihood of splitting damage,characterized by a high degree of discrete and weak stress sensitivity.The major conclusion is that the positive rhythm lithofacies of medium-grained sandstone to siltstone are the most favorable for efficient CO_(2) sequestration in deep unmineable coal seams.展开更多
To address the controversial issue of the toxicity of dental alloys and silver nanoparticles in medical ap- plications, an in vivo-like LO2 3-D model was constructed within polyvinylidene fluoride hollow fiber materia...To address the controversial issue of the toxicity of dental alloys and silver nanoparticles in medical ap- plications, an in vivo-like LO2 3-D model was constructed within polyvinylidene fluoride hollow fiber materials to mimic the microenvironment of liver tissue. The use of microscopy methods and the measurement of liver-specific functions optimized the model for best cell performances and also proved the superiority of the 3-D LO2 model when compared with the traditional monolayer model. Toxicity tests were conducted using the newly constructed model, finding that four dental castings coated with silver nanoparticles were toxic to human hepatocytes after cell viability assays. In general, the toxicity of both the castings and the coated silver nanoparticles aggravated as time increased, yet the nanoparticles attenuated the general toxicity by preventing metal ion release, especially at high concentrations.展开更多
Dihydronicotinamide adenine dinucleotide(NADH)is an important enzyme in all living cells,which is found to be abnormally expressed in cancer cells.Since it is redox-active,an electrochemical detection method would be ...Dihydronicotinamide adenine dinucleotide(NADH)is an important enzyme in all living cells,which is found to be abnormally expressed in cancer cells.Since it is redox-active,an electrochemical detection method would be suitable for monitoring its concentration in biological fluids.Here we present a strategy for specific determination of NADH in real human serum by using RhIr@MoS2 nanohybrids based microsensor.To implement the protocol,RhIr nanocrysrals are in-situ grown onto MoS2 interlayers forming a nanohybrid structure(RhIr@MoS2).After being locally deposited on an electrochemical microsensor,it could be used for the analysis of NADH.The developed RhIr@MoS2 nanohybrids based microsensor possesses the ability for analyzing NADH at the applied potential of 0.07 V(much lower than most reported values).The detection limit is evaluated as low as 1 nmol/L even in bovine serum albumin(BSA)media.In addition,the sampling analysis of human serum from cancer patients and health controls shows that the microsensor displays good diagnostic sensitivity and specificity,illustrating that this developed detection technique is a relatively accurate method for measuring NADH in biological fluids.The proposed electrochemical microsensor assay also owns the benefits of convenience,disposable and easy processing,which make it a great possibility for future point-of-care cancer diagnosis.展开更多
Numerical simulation and experimental study of the Vickers indentation testing of the Al2O3 ceramic coated by diamond-like carbon(DLC) layer were conducted.The numerical analysis was implemented by a two-dimensional f...Numerical simulation and experimental study of the Vickers indentation testing of the Al2O3 ceramic coated by diamond-like carbon(DLC) layer were conducted.The numerical analysis was implemented by a two-dimensional finite element(FE) axis symmetry model.FE analysis results gave insight into the fracture mechanism of DLC films coated on brittle ceramic(Al2O3) substrates.The maximum principal stress field was used to locate the most expected area for crack formation and propagation during the Vickers indentation testing.The results show that the median crack initiates in the interface under indenter,before ring crack occurs as the indenter presses down.Finally,the plastic deformation appears when the indenter penetrates into the substrate.The thicker DLC coating increases the Vickers hardness and fracture toughness.展开更多
BACKGROUND CYP21A2 gene mutations may all cause reduction or loss of 21-hydroxylase activity,leading to development of congenital adrenal hyperplasia(CAH)with different clinical phenotypes.For families with CAH childr...BACKGROUND CYP21A2 gene mutations may all cause reduction or loss of 21-hydroxylase activity,leading to development of congenital adrenal hyperplasia(CAH)with different clinical phenotypes.For families with CAH children,genetic testing of the parents and genetic counseling are recommended to assess the risk of recurrence.CASE SUMMARY We report a case of CAH with a high suspicion before delivery.The risk of the child suffering from CAH during the pregnancy had been underestimated due to the deviation of genetic counseling and genetic testing results.Our report confirmed a CYP21A2 homozygous deletion in this case,CYP21A2 heterozygous deletion in the mother,and a rare 2+0 CYP21A2 deletion in the father.CONCLUSION It is important to analyze the distribution of CYP21A2 gene in the two alleles of parents of children with CAH.展开更多
目的探析老年非致残性脑卒中患者血清dickkopf相关蛋白1(dickkopf-related protein 1,DKK-1)、富亮氨酸α-2糖蛋白1(leucine-richα-2-glycoprotein-1,LRG1)水平变化与认知损害的关系。方法回顾性选择2023年1月至2024年12月衡水市人民...目的探析老年非致残性脑卒中患者血清dickkopf相关蛋白1(dickkopf-related protein 1,DKK-1)、富亮氨酸α-2糖蛋白1(leucine-richα-2-glycoprotein-1,LRG1)水平变化与认知损害的关系。方法回顾性选择2023年1月至2024年12月衡水市人民医院神经内科收治的老年非致残性脑卒中患者114例,根据蒙特利尔认知评估量表(Montreal cognitive assessment,MoCA)评分分为认知损害组35例(MoCA评分<26分)和认知正常组79例(MoCA评分≥26分)。检测DKK-1、LRG1水平,并分析二者水平变化与老年非致残性脑卒中患者认知损害的关系。结果认知损害组年龄、病灶体积及美国国立卫生研究院卒中量表评分明显高于认知正常组,差异有统计学意义(P<0.01)。认知损害组和认知正常组发病2周DKK-1、LRG1水平明显低于入院时,差异有统计学意义(P<0.05)。认知损害组发病2周DKK-1、LRG1水平明显高于认知正常组,差异有统计学意义(P<0.01)。多因素logistic回归分析显示,发病2周DKK-1(OR=1.049,95%CI:1.024~1.074,P<0.01)、LRG1(OR=1.043,95%CI:1.008~1.079,P<0.05)水平、年龄(OR=1.290,95%CI:1.073~1.552,P<0.01)、病灶体积(OR=1.977,95%CI:1.175~3.327,P<0.05)是老年非致残性脑卒中患者发生认知损害的危险因素。ROC曲线分析显示,老年非致残性脑卒中患者发病2周DKK-1、LRG1水平、年龄、病灶体积评估发生认知损害的曲线下面积分别为0.807、0.720、0.661、0.729(P<0.01)。结论老年非致残性脑卒中患者血清DKK-1、LRG1水平持续较高可能与认知损害风险存在一定的关联,临床可借助这两种指标进行辅助评估。展开更多
以松茸[Tricholoma matsutake(S.Ito et Imai)Sing.]超临界提取物为研究对象,采用超临界CO_(2)萃取法制得松茸超临界提取物,使用超高效液相色谱-串联质谱(UPLC-MS)初步分析其成分;通过酪氨酸酶抑制试验、小鼠黑色素瘤细胞(B16-F10)试验...以松茸[Tricholoma matsutake(S.Ito et Imai)Sing.]超临界提取物为研究对象,采用超临界CO_(2)萃取法制得松茸超临界提取物,使用超高效液相色谱-串联质谱(UPLC-MS)初步分析其成分;通过酪氨酸酶抑制试验、小鼠黑色素瘤细胞(B16-F10)试验以及斑马鱼试验,评价其美白功效。结果表明,松茸超临界提取物对酪氨酸酶有一定的抑制活性,在低质量浓度(24.39μg/mL)下抑制率为(23.02%±0.65%),抑制率随质量浓度升高而提升,抑制率最高为(33.96%±1.17%),并且在0.0125 mg/mL时,对B16-F10细胞黑色素分泌的抑制率为(31.14%±0.81%);对斑马鱼中的黑色素形成也具有显著的抑制作用,质量浓度为0.05 mg/mL时可抑制斑马鱼中黑色素(24.83%±0.96%)形成。综上,松茸超临界提取物具有作为美白功效原料的潜力,在美白、提亮肤色方面均有开发价值。展开更多
基金supported by Institute of Information and Communications Technology Planning and Evaluation(IITP)grant funded by the Korean government(MSIT)(No.2019-0-01842,Artificial Intelligence Graduate School Program(GIST))supported by Korea Planning&Evaluation Institute of Industrial Technology(KEIT)grant funded by the Ministry of Trade,Industry&Energy(MOTIE,Republic of Korea)(RS-2025-25448249+1 种基金Automotive Industry Technology Development(R&D)Program)supported by the Regional Innovation System&Education(RISE)programthrough the(Gwangju RISE Center),funded by the Ministry of Education(MOE)and the Gwangju Metropolitan City,Republic of Korea(2025-RISE-05-001).
文摘In real-world autonomous driving tests,unexpected events such as pedestrians or wild animals suddenly entering the driving path can occur.Conducting actual test drives under various weather conditions may also lead to dangerous situations.Furthermore,autonomous vehicles may operate abnormally in bad weather due to limitations of their sensors and GPS.Driving simulators,which replicate driving conditions nearly identical to those in the real world,can drastically reduce the time and cost required for market entry validation;consequently,they have become widely used.In this paper,we design a virtual driving test environment capable of collecting and verifying SiLS data under adverse weather conditions using multi-source images.The proposed method generates a virtual testing environment that incorporates various events,including weather,time of day,and moving objects,that cannot be easily verified in real-world autonomous driving tests.By setting up scenario-based virtual environment events,multi-source image analysis and verification using real-world DCUs(Data Concentrator Units)with V2X-Car edge cloud can effectively address risk factors that may arise in real-world situations.We tested and validated the proposed method with scenarios employing V2X communication and multi-source image analysis.
文摘Human epidermal growth factor receptor 2(HER2) overexpression is increasingly recognized as a frequent molecular abnormality in gastric and gastroesophageal cancer. With the recent introduction of HER2 molecular targeted therapy for patients with advanced gastric cancer, determination of HER2 status is crucial in order to select patients who may benefit from this treatment. This paper provides an update on our knowledge of HER2 in gastric and gastroesophageal cancer, including the prognostic relevance of HER2, the key differences between HER2 protein expression interpretation in breast and gastric cancer, the detection methods and the immunohistochemistry scoring system.
基金funded by the National Natural Science Foundation of China(No.42141013)China Geological Survey(DD20221818,DD20242513).
文摘China has pledged to peak carbon dioxide(CO_(2))emissions by 2030 and achieve carbon neutrality by 2060.Carbon capture and storage(CCS)will play a key role in these efforts.Over the past several years,the China Geological Survey(CGS)has completed the Suitability Evaluation Map of CO_(2)Geological Storage in Main Sedimentary Basins in China and Adjacent Sea Regions in 2017.This map reflects the suitability of the first-and secondary-order tectonic units within sedimentary basins for cO_(2)geological storage for CCS planning.The Junggar Basin is recognized as an important region for future CCS projects.Results from a mesoscale evaluation using the volume method indicate that deep saline aquifers represent the most significant resources for CO_(2)storage,with potential ranging from 48×10^(9)to 164×10^(9)t(with a P50 value of 96×10^(9)t).The highest storage potential is identified in the central and northern parts of the basin,reaching up to 9.5×10^(6)t/km^(2)at the P50 probability level.In contrast,the hinterland,eastern,and western parts of the basin generally exhibit storage potential of below 1.0×10^(6)t/km^(2)at the same probability level.The CGs has also characterized historical CO_(2)plume migration in reservoirs at the storage site of the Shenhua CCS demonstration project and conducted numerical simulations of CO_(2)plume migration for periods of 10 and 20 years following the shutdown of the injection well.The CGS implemented a kiloton-scale pilot test on CO_(2)-enhanced water recovery(CO_(2)-EWR)in eastern Junggar,revealing that CO_(2)flooding can improve the pressure for fluid production,with the highest ratio of CO_(2)to produced fluids estimated at approximately 1.2.Besides,an observation field for natural CO_(2)leakage,covering about 930 m^(2),was built in Qinghai Province.In natural CO_(2)fields or at artificial CO_(2)injection research sites,cO_(2)leakage points are primarily related to the distribution of faults(especially fault crossing),which can serve as pathways for CO_(2)leakage.The observation field provides a natural analog to wellbore failure and offers an opportunity to further monitor CO_(2)geological storage sites.However,it has been inferred that borehole ZK10 at the observation field has become a leakage pathway due to the drilling activities,inadequate well-plugging,and abandonment procedures without considering CO_(2)corrosion.
基金supported by the National Natural Science Foundation of China(Grant Nos.22072031,12372107,11832010,and 11890682)the Strategic Priority Research Program of Chinese Academy of Sciences(Grant No.XDB36000000).
文摘The bulge test is a widely utilized method for assessing the mechanical properties of thin films,including metals,polymers,and semiconductors.However,as film thickness diminishes to nanometer scales,boundary conditions dominated by weak van der Waals forces significantly impact mechanical responses.Instead of sample fracture,interfacial shear deformation and delamination become the primary deformation modes,thereby challenging the applicability of conventional bulge models.To accommodate the interfacial effect,a modified mechanical model based on the bulge test has been proposed.This review summarizes recent advancements in the bulge test to highlight the potential challenges and opportunities for future research.
基金supported by the project from the Exploration and Development Research Institute of PetroChina Daqing Oilfield Companyfinancial support from the research by the National Natural Science Foundation of China(42402148)+1 种基金Sichuan Provincial Fund(24NSFSC4997)Guizhou Outstanding Young Science and Technology Talent Program(YQK[2023]012).
文摘Microstructural heterogeneity of low-permeability sandstone roofs of deep unmineable coal seams due to diagenesis significantly affects rock mechanical behavior,greatly impacting the sealing potential of in situ CO_(2) sequestration and the structural stability of the geological formation.However,little is known about how the microstructure of different mineral groups influences the multiscale mechanical behavior of deep sandstone.This study proposes a new method for quantitatively characterizing the multiscale mechanical properties of low-permeability sandstone and shows the mechanisms responsible for mechanical failure at the micro-,meso-,and macroscale.Triaxial compression tests and targeted nanoindentation tests were conducted to assess the micro-and macroscale mechanical properties of different types of sandstone.The micro-and macroscale experiments were coupled with numerical simulations of compression using a unified cohesive model based on Voronoi polygons to clarify the multiscale mechanical behavior.The results indicate that quartz,the primary mineral component of the sandstones examined,exhibits the strongest micromechanical properties,followed by feldspar,calcite,and clay minerals.Compared to polycrystalline quartz,monocrystalline quartz has a more stable microstructure and is mechanically stronger.The macro-mechanical properties of tight sandstone samples are weakened by increased microstructural inhomogeneity and larger grain size.This leads to a higher likelihood of splitting damage,characterized by a high degree of discrete and weak stress sensitivity.The major conclusion is that the positive rhythm lithofacies of medium-grained sandstone to siltstone are the most favorable for efficient CO_(2) sequestration in deep unmineable coal seams.
基金Project supported by the Zhejiang Provincial Natural Science Foundation of China(No.LZ14C200001)the Public Welfare Project of Science Technology Department of Zhejiang Province(No.2013c33139),China
文摘To address the controversial issue of the toxicity of dental alloys and silver nanoparticles in medical ap- plications, an in vivo-like LO2 3-D model was constructed within polyvinylidene fluoride hollow fiber materials to mimic the microenvironment of liver tissue. The use of microscopy methods and the measurement of liver-specific functions optimized the model for best cell performances and also proved the superiority of the 3-D LO2 model when compared with the traditional monolayer model. Toxicity tests were conducted using the newly constructed model, finding that four dental castings coated with silver nanoparticles were toxic to human hepatocytes after cell viability assays. In general, the toxicity of both the castings and the coated silver nanoparticles aggravated as time increased, yet the nanoparticles attenuated the general toxicity by preventing metal ion release, especially at high concentrations.
基金supported by National Key R&D Program of China(No.2016YFA0200800)Shanghai Science and Technology Innovation Action Plan(No.19520744200)+1 种基金Natural Science Foundation of Shanghai(Nos.17ZR1410000,18ZR1415400)the financial support of State Key Laboratory of Transducer Technology of China(No.SKT1806)。
文摘Dihydronicotinamide adenine dinucleotide(NADH)is an important enzyme in all living cells,which is found to be abnormally expressed in cancer cells.Since it is redox-active,an electrochemical detection method would be suitable for monitoring its concentration in biological fluids.Here we present a strategy for specific determination of NADH in real human serum by using RhIr@MoS2 nanohybrids based microsensor.To implement the protocol,RhIr nanocrysrals are in-situ grown onto MoS2 interlayers forming a nanohybrid structure(RhIr@MoS2).After being locally deposited on an electrochemical microsensor,it could be used for the analysis of NADH.The developed RhIr@MoS2 nanohybrids based microsensor possesses the ability for analyzing NADH at the applied potential of 0.07 V(much lower than most reported values).The detection limit is evaluated as low as 1 nmol/L even in bovine serum albumin(BSA)media.In addition,the sampling analysis of human serum from cancer patients and health controls shows that the microsensor displays good diagnostic sensitivity and specificity,illustrating that this developed detection technique is a relatively accurate method for measuring NADH in biological fluids.The proposed electrochemical microsensor assay also owns the benefits of convenience,disposable and easy processing,which make it a great possibility for future point-of-care cancer diagnosis.
文摘Numerical simulation and experimental study of the Vickers indentation testing of the Al2O3 ceramic coated by diamond-like carbon(DLC) layer were conducted.The numerical analysis was implemented by a two-dimensional finite element(FE) axis symmetry model.FE analysis results gave insight into the fracture mechanism of DLC films coated on brittle ceramic(Al2O3) substrates.The maximum principal stress field was used to locate the most expected area for crack formation and propagation during the Vickers indentation testing.The results show that the median crack initiates in the interface under indenter,before ring crack occurs as the indenter presses down.Finally,the plastic deformation appears when the indenter penetrates into the substrate.The thicker DLC coating increases the Vickers hardness and fracture toughness.
基金Supported by the Fundamental Research Funds of Health Commission of Sichuan Province,No.17ZD035.
文摘BACKGROUND CYP21A2 gene mutations may all cause reduction or loss of 21-hydroxylase activity,leading to development of congenital adrenal hyperplasia(CAH)with different clinical phenotypes.For families with CAH children,genetic testing of the parents and genetic counseling are recommended to assess the risk of recurrence.CASE SUMMARY We report a case of CAH with a high suspicion before delivery.The risk of the child suffering from CAH during the pregnancy had been underestimated due to the deviation of genetic counseling and genetic testing results.Our report confirmed a CYP21A2 homozygous deletion in this case,CYP21A2 heterozygous deletion in the mother,and a rare 2+0 CYP21A2 deletion in the father.CONCLUSION It is important to analyze the distribution of CYP21A2 gene in the two alleles of parents of children with CAH.
文摘目的探析老年非致残性脑卒中患者血清dickkopf相关蛋白1(dickkopf-related protein 1,DKK-1)、富亮氨酸α-2糖蛋白1(leucine-richα-2-glycoprotein-1,LRG1)水平变化与认知损害的关系。方法回顾性选择2023年1月至2024年12月衡水市人民医院神经内科收治的老年非致残性脑卒中患者114例,根据蒙特利尔认知评估量表(Montreal cognitive assessment,MoCA)评分分为认知损害组35例(MoCA评分<26分)和认知正常组79例(MoCA评分≥26分)。检测DKK-1、LRG1水平,并分析二者水平变化与老年非致残性脑卒中患者认知损害的关系。结果认知损害组年龄、病灶体积及美国国立卫生研究院卒中量表评分明显高于认知正常组,差异有统计学意义(P<0.01)。认知损害组和认知正常组发病2周DKK-1、LRG1水平明显低于入院时,差异有统计学意义(P<0.05)。认知损害组发病2周DKK-1、LRG1水平明显高于认知正常组,差异有统计学意义(P<0.01)。多因素logistic回归分析显示,发病2周DKK-1(OR=1.049,95%CI:1.024~1.074,P<0.01)、LRG1(OR=1.043,95%CI:1.008~1.079,P<0.05)水平、年龄(OR=1.290,95%CI:1.073~1.552,P<0.01)、病灶体积(OR=1.977,95%CI:1.175~3.327,P<0.05)是老年非致残性脑卒中患者发生认知损害的危险因素。ROC曲线分析显示,老年非致残性脑卒中患者发病2周DKK-1、LRG1水平、年龄、病灶体积评估发生认知损害的曲线下面积分别为0.807、0.720、0.661、0.729(P<0.01)。结论老年非致残性脑卒中患者血清DKK-1、LRG1水平持续较高可能与认知损害风险存在一定的关联,临床可借助这两种指标进行辅助评估。
