The application of a male-sterile line is an ideal approach for hybrid seed production in non-heading Chinese cabbage(Brassica rapa ssp.chinensis).However,the molecular mechanisms underlying male sterility in B.rapa a...The application of a male-sterile line is an ideal approach for hybrid seed production in non-heading Chinese cabbage(Brassica rapa ssp.chinensis).However,the molecular mechanisms underlying male sterility in B.rapa are still largely unclear.We previously obtained the natural male sterile line WS24-3 of non-heading Chinese cabbage and located the male sterile locus,Bra2Ms,on the A2 chromosome.Cytological observations revealed that the male sterility of WS24-3 resulted from disruption of the meiosis process during pollen formation.Fine mapping of Bra2Ms delimited the locus within a physical distance of about 129 kb on the A2 chromosome of B.rapa.The Bra039753 gene encodes a plant homeodomain(PHD)-finger protein and is considered a potential candidate gene for Bra2Ms.Bra039753 was significantly downregulated in sterile line WS24-3 compared to the fertile line at the meiotic anther stage.Sequence analysis of Bra039753 identified a 369 bp fragment insertion in the first exon in male sterile plants,which led to an amino acid insertion in the Bra039753 protein.In addition,the 369 bp fragment insertion was found to cosegregate with the male sterility trait.This study identified a novel locus related to male sterility in non-heading Chinese cabbage,and the molecular marker obtained in this study will be beneficial for the marker-assisted selection of excellent sterile lines in non-heading Chinese cabbage and other Brassica crops.展开更多
为探究软质布敦岩沥青(Buton Rock Asphalt,BRA)作为沥青改性和再生双重功效添加剂的效果,以常用的中面层沥青混合料AC-20C为研究对象,基于响应面优化法设计室内试验,对不同软质BRA掺量(1%~5%)及不同沥青混合料回收料(Reclaimed Asphalt...为探究软质布敦岩沥青(Buton Rock Asphalt,BRA)作为沥青改性和再生双重功效添加剂的效果,以常用的中面层沥青混合料AC-20C为研究对象,基于响应面优化法设计室内试验,对不同软质BRA掺量(1%~5%)及不同沥青混合料回收料(Reclaimed Asphalt Pavement,RAP)掺量(10%~50%)的改性热再生混合料进行组配设计与性能检验,提出了软质BRA的最佳掺量;基于此,通过不同RAP掺量的软质BRA改性热再生混合料的路用性能验证,并与普通沥青混合料进行对比,确定了适宜的RAP掺量范围。研究结果表明:软质BRA中纯沥青的稠度及高、低温性能介于A-70^(#)基质沥青与硬质BRA纯沥青及RAP旧沥青之间,具有良好的黏弹性和较多的轻质组分,可作为再生剂使用;随着软质BRA和RAP掺量的增加,改性热再生混合料的总最佳油石比增大,而基质沥青最佳油石比减小,即软质BRA可替代部分基质沥青,推荐其最佳掺量为3%;与普通混合料相比,当RAP掺量不超过30%时,软质BRA的掺入能综合提高改性热再生混合料的高温稳定性、水稳定性、低温抗裂性能和抗渗性能,可作为改性剂使用,并建议RAP的适宜掺量为10%~30%。因此,软质BRA可作为一种新型经济环保的绿色改性再生剂用于沥青路面厂拌热再生工程。展开更多
搅拌研磨工艺显著提升了布敦岩沥青(Buton Rock Asphalt,BRA)与沥青共混体系的稳定性,但BRA的矿物粒径分布对改性沥青流变性能的影响机制尚不清晰。为此,该文通过制备不同粒径分布的BRA改性沥青样品,开展高低温流变试验,系统分析BRA粒...搅拌研磨工艺显著提升了布敦岩沥青(Buton Rock Asphalt,BRA)与沥青共混体系的稳定性,但BRA的矿物粒径分布对改性沥青流变性能的影响机制尚不清晰。为此,该文通过制备不同粒径分布的BRA改性沥青样品,开展高低温流变试验,系统分析BRA粒径分布对BRA改性沥青流变行为的影响,同时结合荧光显微试验,分析了不同粒径分布下的BRA改性沥青的相态结构。结果表明:D_(50)、D_(90)和D_(span)与改性沥青的高温抗变形能力呈负相关,而与低温蠕变刚度和蠕变速率呈正相关,这表明减小BRA粒径有助于提升改性沥青的高温稳定性和低温抗裂性;减小无机矿物的粒径能够改善无机矿物在沥青中的分散状态,使结构更致密,从而增强高温抗车辙性能与低温柔韧性。展开更多
Wireless Sensor Networks(WSNs)have emerged as crucial tools for real-time environmental monitoring through distributed sensor nodes(SNs).However,the operational lifespan of WSNs is significantly constrained by the lim...Wireless Sensor Networks(WSNs)have emerged as crucial tools for real-time environmental monitoring through distributed sensor nodes(SNs).However,the operational lifespan of WSNs is significantly constrained by the limited energy resources of SNs.Current energy efficiency strategies,such as clustering,multi-hop routing,and data aggregation,face challenges,including uneven energy depletion,high computational demands,and suboptimal cluster head(CH)selection.To address these limitations,this paper proposes a hybrid methodology that optimizes energy consumption(EC)while maintaining network performance.The proposed approach integrates the Low Energy Adaptive Clustering Hierarchy with Deterministic(LEACH-D)protocol using an Artificial Neural Network(ANN)and Bayesian Regularization Algorithm(BRA).LEACH-D improves upon conventional LEACH by ensuring more uniform energy usage across SNs,mitigating inefficiencies from random CH selection.The ANN further enhances CH selection and routing processes,effectively reducing data transmission overhead and idle listening.Simulation results reveal that the LEACH-D-ANN model significantly reduces EC and extends the network’s lifespan compared to existing protocols.This framework offers a promising solution to the energy efficiency challenges in WSNs,paving the way for more sustainable and reliable network deployments.展开更多
基金We thank the Wuhan Major Project of Key Technologies in Biological Breeding and New Variety Cultivation,China(2022021302024852)The Science and Technology Support Project of Rural Vitalization in Hubei Province,China(2022BBA121)+1 种基金the Key Research and Development Project of Hubei Province,China(2021BBA097)The Key Research and Development Project of Hubei Province,China(2021BBA102)。
文摘The application of a male-sterile line is an ideal approach for hybrid seed production in non-heading Chinese cabbage(Brassica rapa ssp.chinensis).However,the molecular mechanisms underlying male sterility in B.rapa are still largely unclear.We previously obtained the natural male sterile line WS24-3 of non-heading Chinese cabbage and located the male sterile locus,Bra2Ms,on the A2 chromosome.Cytological observations revealed that the male sterility of WS24-3 resulted from disruption of the meiosis process during pollen formation.Fine mapping of Bra2Ms delimited the locus within a physical distance of about 129 kb on the A2 chromosome of B.rapa.The Bra039753 gene encodes a plant homeodomain(PHD)-finger protein and is considered a potential candidate gene for Bra2Ms.Bra039753 was significantly downregulated in sterile line WS24-3 compared to the fertile line at the meiotic anther stage.Sequence analysis of Bra039753 identified a 369 bp fragment insertion in the first exon in male sterile plants,which led to an amino acid insertion in the Bra039753 protein.In addition,the 369 bp fragment insertion was found to cosegregate with the male sterility trait.This study identified a novel locus related to male sterility in non-heading Chinese cabbage,and the molecular marker obtained in this study will be beneficial for the marker-assisted selection of excellent sterile lines in non-heading Chinese cabbage and other Brassica crops.
文摘为探究软质布敦岩沥青(Buton Rock Asphalt,BRA)作为沥青改性和再生双重功效添加剂的效果,以常用的中面层沥青混合料AC-20C为研究对象,基于响应面优化法设计室内试验,对不同软质BRA掺量(1%~5%)及不同沥青混合料回收料(Reclaimed Asphalt Pavement,RAP)掺量(10%~50%)的改性热再生混合料进行组配设计与性能检验,提出了软质BRA的最佳掺量;基于此,通过不同RAP掺量的软质BRA改性热再生混合料的路用性能验证,并与普通沥青混合料进行对比,确定了适宜的RAP掺量范围。研究结果表明:软质BRA中纯沥青的稠度及高、低温性能介于A-70^(#)基质沥青与硬质BRA纯沥青及RAP旧沥青之间,具有良好的黏弹性和较多的轻质组分,可作为再生剂使用;随着软质BRA和RAP掺量的增加,改性热再生混合料的总最佳油石比增大,而基质沥青最佳油石比减小,即软质BRA可替代部分基质沥青,推荐其最佳掺量为3%;与普通混合料相比,当RAP掺量不超过30%时,软质BRA的掺入能综合提高改性热再生混合料的高温稳定性、水稳定性、低温抗裂性能和抗渗性能,可作为改性剂使用,并建议RAP的适宜掺量为10%~30%。因此,软质BRA可作为一种新型经济环保的绿色改性再生剂用于沥青路面厂拌热再生工程。
文摘搅拌研磨工艺显著提升了布敦岩沥青(Buton Rock Asphalt,BRA)与沥青共混体系的稳定性,但BRA的矿物粒径分布对改性沥青流变性能的影响机制尚不清晰。为此,该文通过制备不同粒径分布的BRA改性沥青样品,开展高低温流变试验,系统分析BRA粒径分布对BRA改性沥青流变行为的影响,同时结合荧光显微试验,分析了不同粒径分布下的BRA改性沥青的相态结构。结果表明:D_(50)、D_(90)和D_(span)与改性沥青的高温抗变形能力呈负相关,而与低温蠕变刚度和蠕变速率呈正相关,这表明减小BRA粒径有助于提升改性沥青的高温稳定性和低温抗裂性;减小无机矿物的粒径能够改善无机矿物在沥青中的分散状态,使结构更致密,从而增强高温抗车辙性能与低温柔韧性。
文摘Wireless Sensor Networks(WSNs)have emerged as crucial tools for real-time environmental monitoring through distributed sensor nodes(SNs).However,the operational lifespan of WSNs is significantly constrained by the limited energy resources of SNs.Current energy efficiency strategies,such as clustering,multi-hop routing,and data aggregation,face challenges,including uneven energy depletion,high computational demands,and suboptimal cluster head(CH)selection.To address these limitations,this paper proposes a hybrid methodology that optimizes energy consumption(EC)while maintaining network performance.The proposed approach integrates the Low Energy Adaptive Clustering Hierarchy with Deterministic(LEACH-D)protocol using an Artificial Neural Network(ANN)and Bayesian Regularization Algorithm(BRA).LEACH-D improves upon conventional LEACH by ensuring more uniform energy usage across SNs,mitigating inefficiencies from random CH selection.The ANN further enhances CH selection and routing processes,effectively reducing data transmission overhead and idle listening.Simulation results reveal that the LEACH-D-ANN model significantly reduces EC and extends the network’s lifespan compared to existing protocols.This framework offers a promising solution to the energy efficiency challenges in WSNs,paving the way for more sustainable and reliable network deployments.
