Plants have developed a multi-layered immune system to cope with pathogens.The receptors on the plasma membrane are controlled by endocytosis to modulate immune signaling,but the regulatory mechanisms of endocytosis i...Plants have developed a multi-layered immune system to cope with pathogens.The receptors on the plasma membrane are controlled by endocytosis to modulate immune signaling,but the regulatory mechanisms of endocytosis in this process remain largely unclear.Here,we uncover that reversible S-acylation of BONZAl1(BON1),a conserved copine-family protein that regulates development-immunity balance in Arabidopsis,contributes to the accurate control of endocytosis.BON1 is targeted by S-acylation,a type of protein lipidation,for its localization on the plasma membrane and its function in development and immunity.Furthermore,the S-acylation status of BON1affects its association with the light-chain clathrin subunitCLC3 and regulates endocytosis.Specifically,PAT14 facilitates the S-acylation of BON1,while ABAPT11 mediates its de-S-acylation.Physiological levels of reversible S-acylation of BON1 are essential for endocytosis and the internalization of immune receptors.Interestingly,salicylic acid enhances ABAPT11-dependent de-S-acylation of BON1 to amplify immune signaling.Collectively,our study reveals that reversible S-acylation of BON1 precisely regulates immune receptor internalization for balancing plant development and immunity,providing potential targets that may be used to improve crop yields and disease resistance.展开更多
基金supported by National Natural Science Foundation of China(32270752,32270292,and 32400227)Major Program of Guangdong Basic and Applied Research(2019B030302006)+3 种基金Natural Science Foundation of Guangdong,China(2024A1515011071 and 2023A1515110948)Guangdong Modern Agro-industry Technology Research System(2023KJ114)the Program for Changjiang Scholars,the China Post doctoral Science Foundation(2023M741236)the Postdoctoral Fellowship Program of CPSF(GZB20240238).
文摘Plants have developed a multi-layered immune system to cope with pathogens.The receptors on the plasma membrane are controlled by endocytosis to modulate immune signaling,but the regulatory mechanisms of endocytosis in this process remain largely unclear.Here,we uncover that reversible S-acylation of BONZAl1(BON1),a conserved copine-family protein that regulates development-immunity balance in Arabidopsis,contributes to the accurate control of endocytosis.BON1 is targeted by S-acylation,a type of protein lipidation,for its localization on the plasma membrane and its function in development and immunity.Furthermore,the S-acylation status of BON1affects its association with the light-chain clathrin subunitCLC3 and regulates endocytosis.Specifically,PAT14 facilitates the S-acylation of BON1,while ABAPT11 mediates its de-S-acylation.Physiological levels of reversible S-acylation of BON1 are essential for endocytosis and the internalization of immune receptors.Interestingly,salicylic acid enhances ABAPT11-dependent de-S-acylation of BON1 to amplify immune signaling.Collectively,our study reveals that reversible S-acylation of BON1 precisely regulates immune receptor internalization for balancing plant development and immunity,providing potential targets that may be used to improve crop yields and disease resistance.
