AIM: Liver fibrosis is a common pathological process of chronic liver diseases. Activation of hepatic stellate cells(HSCs) is the key issue in the occurrence of liver fibrosis. In this study, we observed the inhibitor...AIM: Liver fibrosis is a common pathological process of chronic liver diseases. Activation of hepatic stellate cells(HSCs) is the key issue in the occurrence of liver fibrosis. In this study, we observed the inhibitory action of rat serum containing Biejiajian oral liquid (BOL), a decoction of turtle shell, on proliferation of rat HSCs, and to explore the antihepatofibrotic mechanisms of BOL.METHODS: A rat model of hepatic fibrosis was induced by subcutaneous injection of CC14. Serum containing low,medium and high dosages of BOL was prepared respectively.Normal and fibrotic HSCs were isolated and cultured. The effect of sera containing BOL on proliferation of HSCs was determined by 3H-TdR incorporation.RESULTS: The inhibitory rate of normal rat HSC proliferation caused by 100 mL/mL sera containing medium and high dosages of BOL showed a remarkable difference as compared with that caused by colchicine (medium dosage group:34.56±4.21% vs29.12±2.85%, P<0.01; high dosage group:37.82±1.32% vs29.12±2.85%, P<0.01). The inhibitory rate of fibrotic rat HSC proliferation caused by 100 mL/L serum containing medium and high dosages of BOL showed a remarkable difference as compared with that caused by colchicine (medium dosage group: 51.31_+3.14% vs 38.32_+2.65%,P<0.01; high dosage group: 60.15_+5.36% vs38.32_+2.65%,P<0.01). The inhibitory rate of normal rat HSC proliferation caused by 100 mL/L and 200 mL/L sera containing a medium dosage of BOL showed a significant difference as compared with that caused by 50 mL/L (100 mL/L group: 69.02±9.96%vs 50.82±9.28%, P<0.05; 200 mL/L group: 81.78±8.92%vs50.82±9.28%, P<0.01). The inhibitory rate of fibrotic rat HSC proliferation caused by 100 mL/L and 200 mL/L sera containing a medium dosage of BOL showed a significant difference as compared with that caused by 50 mL/L (100 mL/L group:72.19±10.96% vs 61.38±7.16%, P<0.05; 200 mL/L group:87.16±8.54% vs 61.38±7.16%, P<0.01).CONCLUSION: Rat serum containing BOL can inhibit proliferation of rat HSCs, and the inhibition depends on the dosage and concentration of BOL. The inhibitory effect on HSC proliferation is one of the main anti-hepatofibroticmechanisms of BOL.展开更多
Two centuries ago,Simón Bolivar penned his legendary Letter from Jamaica,in which he warned that the greatest danger for Latin America after independence would come not only from foreign powers,but also from its ...Two centuries ago,Simón Bolivar penned his legendary Letter from Jamaica,in which he warned that the greatest danger for Latin America after independence would come not only from foreign powers,but also from its own divisions.Newly independent states,if fractured and mistrustful,would remain vulnerable to new forms of domination.Unity,for Bolivar,was not a slogan,but a condition of survival.展开更多
Atomic-undercoordination-induced local bond contraction,bond strength gain,and the associated temperature (T)-dependent atomic-cohesive-energy and binding-energy-density are shown to originate intrinsically the exotic...Atomic-undercoordination-induced local bond contraction,bond strength gain,and the associated temperature (T)-dependent atomic-cohesive-energy and binding-energy-density are shown to originate intrinsically the exotic paradox of superplasticity,superelasticity,and superrigidity demonstrated by solid sizing from monatomic chain to mesoscopic grain.The paradox follows these relationships:(ε(K,T)y(K,T)σ(K,T))∝(exp(B/△T_(mk)),(η_1△T_(mk))d～(-3),[1+AK～(-2/2)exp(△T_(mk)/T)]△T_(mk)d～(-3)),(Plastic strain)(Elastic modulus)(Yield stress,IHPR)where A,B,η1,d and△T_(mk)=Tm(K) Tare size (K)-dependent physical parameters.Tm (K) is the melting point.Mechanical work hardening during compressing and self-heating during stretching modulate the measured outcome extrinsically.Superplasticity dominates in the solid-quasimolten-liquid transition state.The competition between the accumulation and annihilation of dislocations activates the inverse Hall-Petch relationship.Therefore,it is essential for one to discriminate the intrinsic competition between the local bond energy density gain and the atomic cohesive energy loss from the extrinsic factors of pressure and temperature in dealing with atomistic mechano-thermo dynamics.展开更多
Dear Friends,Our learning process is complicated.It takes many forms.Welearn from our family and from our friends.We learn from ourreadings and from our teachers.We learn from our experiences,and we learn from the exp...Dear Friends,Our learning process is complicated.It takes many forms.Welearn from our family and from our friends.We learn from ourreadings and from our teachers.We learn from our experiences,and we learn from the experiences of others.In my many years oflife,I have learned from all of these sources.In this column let me share with you a few of the"learnings"that I remember most at this moment.These are presented in nological order.They are merely bits of wisdom and how I interpretthem.展开更多
基金Supported by the Natural Science Foundation of Zhejiang Province,No.398402
文摘AIM: Liver fibrosis is a common pathological process of chronic liver diseases. Activation of hepatic stellate cells(HSCs) is the key issue in the occurrence of liver fibrosis. In this study, we observed the inhibitory action of rat serum containing Biejiajian oral liquid (BOL), a decoction of turtle shell, on proliferation of rat HSCs, and to explore the antihepatofibrotic mechanisms of BOL.METHODS: A rat model of hepatic fibrosis was induced by subcutaneous injection of CC14. Serum containing low,medium and high dosages of BOL was prepared respectively.Normal and fibrotic HSCs were isolated and cultured. The effect of sera containing BOL on proliferation of HSCs was determined by 3H-TdR incorporation.RESULTS: The inhibitory rate of normal rat HSC proliferation caused by 100 mL/mL sera containing medium and high dosages of BOL showed a remarkable difference as compared with that caused by colchicine (medium dosage group:34.56±4.21% vs29.12±2.85%, P<0.01; high dosage group:37.82±1.32% vs29.12±2.85%, P<0.01). The inhibitory rate of fibrotic rat HSC proliferation caused by 100 mL/L serum containing medium and high dosages of BOL showed a remarkable difference as compared with that caused by colchicine (medium dosage group: 51.31_+3.14% vs 38.32_+2.65%,P<0.01; high dosage group: 60.15_+5.36% vs38.32_+2.65%,P<0.01). The inhibitory rate of normal rat HSC proliferation caused by 100 mL/L and 200 mL/L sera containing a medium dosage of BOL showed a significant difference as compared with that caused by 50 mL/L (100 mL/L group: 69.02±9.96%vs 50.82±9.28%, P<0.05; 200 mL/L group: 81.78±8.92%vs50.82±9.28%, P<0.01). The inhibitory rate of fibrotic rat HSC proliferation caused by 100 mL/L and 200 mL/L sera containing a medium dosage of BOL showed a significant difference as compared with that caused by 50 mL/L (100 mL/L group:72.19±10.96% vs 61.38±7.16%, P<0.05; 200 mL/L group:87.16±8.54% vs 61.38±7.16%, P<0.01).CONCLUSION: Rat serum containing BOL can inhibit proliferation of rat HSCs, and the inhibition depends on the dosage and concentration of BOL. The inhibitory effect on HSC proliferation is one of the main anti-hepatofibroticmechanisms of BOL.
文摘Two centuries ago,Simón Bolivar penned his legendary Letter from Jamaica,in which he warned that the greatest danger for Latin America after independence would come not only from foreign powers,but also from its own divisions.Newly independent states,if fractured and mistrustful,would remain vulnerable to new forms of domination.Unity,for Bolivar,was not a slogan,but a condition of survival.
基金supports from the National Natural Science Foundation of China(Grant Nos. 11002121,11102176 and 11172254)
文摘Atomic-undercoordination-induced local bond contraction,bond strength gain,and the associated temperature (T)-dependent atomic-cohesive-energy and binding-energy-density are shown to originate intrinsically the exotic paradox of superplasticity,superelasticity,and superrigidity demonstrated by solid sizing from monatomic chain to mesoscopic grain.The paradox follows these relationships:(ε(K,T)y(K,T)σ(K,T))∝(exp(B/△T_(mk)),(η_1△T_(mk))d～(-3),[1+AK～(-2/2)exp(△T_(mk)/T)]△T_(mk)d～(-3)),(Plastic strain)(Elastic modulus)(Yield stress,IHPR)where A,B,η1,d and△T_(mk)=Tm(K) Tare size (K)-dependent physical parameters.Tm (K) is the melting point.Mechanical work hardening during compressing and self-heating during stretching modulate the measured outcome extrinsically.Superplasticity dominates in the solid-quasimolten-liquid transition state.The competition between the accumulation and annihilation of dislocations activates the inverse Hall-Petch relationship.Therefore,it is essential for one to discriminate the intrinsic competition between the local bond energy density gain and the atomic cohesive energy loss from the extrinsic factors of pressure and temperature in dealing with atomistic mechano-thermo dynamics.
文摘Dear Friends,Our learning process is complicated.It takes many forms.Welearn from our family and from our friends.We learn from ourreadings and from our teachers.We learn from our experiences,and we learn from the experiences of others.In my many years oflife,I have learned from all of these sources.In this column let me share with you a few of the"learnings"that I remember most at this moment.These are presented in nological order.They are merely bits of wisdom and how I interpretthem.
