Mushrooms in the basidiomycete family Boletaceae are ecologically and economically very important.However,due to the morphological complexity and the limited phylogenetic information on the various species and genera ...Mushrooms in the basidiomycete family Boletaceae are ecologically and economically very important.However,due to the morphological complexity and the limited phylogenetic information on the various species and genera of this fungal family,our understanding of its systematics and evolution remains rudimentary.In this study,DNA sequences of four genes(nrLSU,tef1-α,rpb1,and rpb2)were newly obtained from ca.200 representative specimens of Boletaceae.Our phylogenetic analyses revealed seven major clades at the subfamily level,namely Austroboletoideae,Boletoideae,Chalciporoideae,Leccinoideae,Xerocomoideae,Zangioideae,and the Pulveroboletus Group.In addition,59 genus-level clades were identified,of which 22 were uncovered for the first time.These 22 clades were mainly placed in Boletoideae and the Pulveroboletus Group.The results further indicated that the characters frequently used in the morphology-based taxonomy of Boletaceae,such as basidiospore ornamentation,the form of the basidioma,and the stuffed pores each had multiple origins within the family,suggesting that the use of such features for high-level classification of Boletaceae should be de-emphasized and combined with other characters.展开更多
We present a revised molecular phylogeny of higher Basidiomycota focusing on Lepidostromataceae based on the large subunit(28S)of the nuclear ribosomal rDNA(nuLSU),with additionl data from the translation elongation f...We present a revised molecular phylogeny of higher Basidiomycota focusing on Lepidostromataceae based on the large subunit(28S)of the nuclear ribosomal rDNA(nuLSU),with additionl data from the translation elongation factor 1 alpha 1(TEF1)and the RNA polymerase II second largest subunit(RPB2)genes.Our results suggest that Lepidostromataceae is best recognized in a separate order,Lepidostromatales ordo novum,within subclass Agaricomycetidae.Furthermore,the internal topology of Lepidostromataceae,correlating with thallus features,indicates that three genera,instead of a single genus,should be recognized.We therefore introduce the genera Ertzia genus novum and Sulzbacheromyces genus novum for Lepidostroma akagerae and L.caatingae,respectively.In addition,the new species L.winklerianum spec.nova is described for Mexican material previously identified as L.calocerum.The photobionts of Sulzbacheromyces and Lepidostroma were identified using molecular data of the large subunit of the ribulose 1,5-bisphosphate carboxylase/oxygenase(rbcL)gene,revealing a possibly undescribed genus in Trebouxiophyceae and the first report of lichenization for the genus Bracteacoccus in Chlorophyceae.展开更多
基金supported by the Funds for International Cooperation and Exchange of the National Natural Science Foundation of China(31210103919)the National Basic Research Program of China(2014CB138305)the CAS/SAFEA International Partnership Program for Creative Research Teams.
文摘Mushrooms in the basidiomycete family Boletaceae are ecologically and economically very important.However,due to the morphological complexity and the limited phylogenetic information on the various species and genera of this fungal family,our understanding of its systematics and evolution remains rudimentary.In this study,DNA sequences of four genes(nrLSU,tef1-α,rpb1,and rpb2)were newly obtained from ca.200 representative specimens of Boletaceae.Our phylogenetic analyses revealed seven major clades at the subfamily level,namely Austroboletoideae,Boletoideae,Chalciporoideae,Leccinoideae,Xerocomoideae,Zangioideae,and the Pulveroboletus Group.In addition,59 genus-level clades were identified,of which 22 were uncovered for the first time.These 22 clades were mainly placed in Boletoideae and the Pulveroboletus Group.The results further indicated that the characters frequently used in the morphology-based taxonomy of Boletaceae,such as basidiospore ornamentation,the form of the basidioma,and the stuffed pores each had multiple origins within the family,suggesting that the use of such features for high-level classification of Boletaceae should be de-emphasized and combined with other characters.
基金Funding was provided to BPH through an Explorers Club Diversa Award,a Duke University Graduate International Research Travel Award,an American Bryological&Lichenological Society Student Travel Award,and a Sigma Xi Grant-In-Aid of ResearchPartial financial support for RL was provided by grant DEB 0841405 from the National Science Foundation to George Mason University:"Phylogenetic Diversity of Mycobionts and Photobionts in the Cyanolichen Genus Dictyonema,with Emphasis on the Neotropics and the Galapagos Islands"(PI:J.Lawrey+1 种基金Co-PIs:R.Lücking,P.Gillevet)Molecular sequence work by BM was partially supported by grant DEB 715660 from the National Science Foundation to The Field Museum:"Neotropical Epiphytic Microlichens-An Innovative Inventory of a Highly Diverse yet Little Known Group of Symbiotic Organisms"(PI R.Lücking).
文摘We present a revised molecular phylogeny of higher Basidiomycota focusing on Lepidostromataceae based on the large subunit(28S)of the nuclear ribosomal rDNA(nuLSU),with additionl data from the translation elongation factor 1 alpha 1(TEF1)and the RNA polymerase II second largest subunit(RPB2)genes.Our results suggest that Lepidostromataceae is best recognized in a separate order,Lepidostromatales ordo novum,within subclass Agaricomycetidae.Furthermore,the internal topology of Lepidostromataceae,correlating with thallus features,indicates that three genera,instead of a single genus,should be recognized.We therefore introduce the genera Ertzia genus novum and Sulzbacheromyces genus novum for Lepidostroma akagerae and L.caatingae,respectively.In addition,the new species L.winklerianum spec.nova is described for Mexican material previously identified as L.calocerum.The photobionts of Sulzbacheromyces and Lepidostroma were identified using molecular data of the large subunit of the ribulose 1,5-bisphosphate carboxylase/oxygenase(rbcL)gene,revealing a possibly undescribed genus in Trebouxiophyceae and the first report of lichenization for the genus Bracteacoccus in Chlorophyceae.
