Dysregulated RNA splicing events produce transcripts that facilitate esophageal squamous cell carcinoma(ESCC)progression,but how this splicing process is abnormally regulated remains elusive.Here,we unveiled a novel a...Dysregulated RNA splicing events produce transcripts that facilitate esophageal squamous cell carcinoma(ESCC)progression,but how this splicing process is abnormally regulated remains elusive.Here,we unveiled a novel alternative splicing axis of BOLA3 transcripts and its regulator HNRNPC in ESCC.The long-form BOLA3(BOLA3-L)containing exon 3 exhibited high expression levels in ESCC and was associated with poor prognosis.Functional assays demonstrated the protumorigenic function of BOLA3-L in ESCC cells.Additionally,HNRNPC bound to BOLA3 mRNA and promoted BOLA3 exon 3 inclusion forming BOLA3-L.High HNRNPC expression was positively correlated with the presence of BOLA3-L and associated with an unfavorable prognosis.HNRNPC knockdown effectively suppressed the malignant biological behavior of ESCC cells,which were significantly rescued by BOLA3-L overexpression.Moreover,BOLA3-L played a significant role in mitochondrial structural and functional stability.E2F7 acted as a key transcription factor that promoted the upregulation of HNRNPC and inclusion of BOLA3 exon 3.Our findings provided novel insights into how alternative splicing contributes to ESCC progression.展开更多
利用PCR-RFLP技术对易感乳房炎的奶牛血样24份,不易感染乳房炎的2个黄牛品种南阳牛、秦川牛血样66份共计90个个体进行牛BoL A-DRB3基因第2外显子多态性分析。结果表明:扩增大小为252 bp的片段经限制性内切酶B stU I酶切后表现多态,且奶...利用PCR-RFLP技术对易感乳房炎的奶牛血样24份,不易感染乳房炎的2个黄牛品种南阳牛、秦川牛血样66份共计90个个体进行牛BoL A-DRB3基因第2外显子多态性分析。结果表明:扩增大小为252 bp的片段经限制性内切酶B stU I酶切后表现多态,且奶牛AA型个体显著高于黄牛品种,提示BoL A等位基因A作为母牛乳房炎易感性的候选标记具有潜在的有效性。展开更多
基金supported by Shanghai Science and Technology Innovation Action Program(No.21Y31900100).
文摘Dysregulated RNA splicing events produce transcripts that facilitate esophageal squamous cell carcinoma(ESCC)progression,but how this splicing process is abnormally regulated remains elusive.Here,we unveiled a novel alternative splicing axis of BOLA3 transcripts and its regulator HNRNPC in ESCC.The long-form BOLA3(BOLA3-L)containing exon 3 exhibited high expression levels in ESCC and was associated with poor prognosis.Functional assays demonstrated the protumorigenic function of BOLA3-L in ESCC cells.Additionally,HNRNPC bound to BOLA3 mRNA and promoted BOLA3 exon 3 inclusion forming BOLA3-L.High HNRNPC expression was positively correlated with the presence of BOLA3-L and associated with an unfavorable prognosis.HNRNPC knockdown effectively suppressed the malignant biological behavior of ESCC cells,which were significantly rescued by BOLA3-L overexpression.Moreover,BOLA3-L played a significant role in mitochondrial structural and functional stability.E2F7 acted as a key transcription factor that promoted the upregulation of HNRNPC and inclusion of BOLA3 exon 3.Our findings provided novel insights into how alternative splicing contributes to ESCC progression.
