动脉性肺动脉高压(Pulmonary Arterial Hypertension, PAH),即第1大类肺动脉高压,是一种以高发病率和死亡率为特点的血管疾病,其主要表现是肺血管重塑和肺血管阻力增加,最终导致右心室衰竭甚至死亡。骨形态发生蛋白9 (Bone Morphogeneti...动脉性肺动脉高压(Pulmonary Arterial Hypertension, PAH),即第1大类肺动脉高压,是一种以高发病率和死亡率为特点的血管疾病,其主要表现是肺血管重塑和肺血管阻力增加,最终导致右心室衰竭甚至死亡。骨形态发生蛋白9 (Bone Morphogenetic Protein 9, BMP9)属于转化生长因子β (Transforming Growth Factor-β, TGF-β)家族,主要由肝脏星状细胞产生,随血液循环到肺血管内皮细胞上与受体结合,在PAH中发挥相应的生物学效应,但是部分研究结果是相互矛盾的。本文对PAH中BMP9的信号通路及BMP9对肺血管内皮细胞作用的研究和进展进行综述。Arterial pulmonary hypertension (PAH), also known as the group 1 pulmonary hypertension, is a vascular disease characterized by high morbidity and mortality, and its main manifestations are pulmonary vascular remodeling and increased pulmonary vascular resistance, which eventually leads to right ventricular failure and even death. Bone Morphogenetic Protein 9 (BMP9) belongs to the Transforming Growth Factor-β (TGF-β) family, which is mainly produced by hepatic stellate cells, which circulates to pulmonary vascular endothelial cells with blood and bind to their receptors to exert corresponding biological effects in PAH, but some research results are contradictory. This article briefly reviews the BMP9 signaling pathway in PAH and the research and progress of BMP9 on pulmonary vascular endothelial cells.展开更多
以骨形态发生蛋白9(bone morphogenetic protein 9,BMP9)作为诱导小鼠间充质干细胞C3H10T1/2定向成骨分化的细胞因子,观察过表达S100A6对成骨分化的影响。用重组腺病毒AdBMP9与AdS100A6共感染C3H10T1/2细胞,随后检测成骨分化标志物,包括...以骨形态发生蛋白9(bone morphogenetic protein 9,BMP9)作为诱导小鼠间充质干细胞C3H10T1/2定向成骨分化的细胞因子,观察过表达S100A6对成骨分化的影响。用重组腺病毒AdBMP9与AdS100A6共感染C3H10T1/2细胞,随后检测成骨分化标志物,包括Runx2、碱性磷酸酶(alkaline phosphatase,ALP)、骨桥素(osteopontin,OPN)和钙盐沉积;检测方法包括免疫细胞化学、ALP染色、活性分析以及茜素红S染色;同时,用Western blot检测β-catenin的表达。过表达S100A6对所诱导的间充质干细胞C3H10T1/2成骨分化的早期指标Runx2的蛋白水平无明显影响(P>0.05;ICC法),对第7天的ALP和第18天钙盐的沉积也无明显影响(P>0.05);但是,能够引起第12天的OPN升高(P<0.05);同时,S100A6对BMP9诱导的C3H10T1/2细胞中的β-catenin水平的影响在第3、7天不明显(P>0.05),但在第12天时可致β-catenin水平下调(P<0.05)。过表达S100A6可促进BMP9诱导的C3H10T1/2细胞中OPN的表达,但对最终的成骨分化无明显影响。展开更多
文摘动脉性肺动脉高压(Pulmonary Arterial Hypertension, PAH),即第1大类肺动脉高压,是一种以高发病率和死亡率为特点的血管疾病,其主要表现是肺血管重塑和肺血管阻力增加,最终导致右心室衰竭甚至死亡。骨形态发生蛋白9 (Bone Morphogenetic Protein 9, BMP9)属于转化生长因子β (Transforming Growth Factor-β, TGF-β)家族,主要由肝脏星状细胞产生,随血液循环到肺血管内皮细胞上与受体结合,在PAH中发挥相应的生物学效应,但是部分研究结果是相互矛盾的。本文对PAH中BMP9的信号通路及BMP9对肺血管内皮细胞作用的研究和进展进行综述。Arterial pulmonary hypertension (PAH), also known as the group 1 pulmonary hypertension, is a vascular disease characterized by high morbidity and mortality, and its main manifestations are pulmonary vascular remodeling and increased pulmonary vascular resistance, which eventually leads to right ventricular failure and even death. Bone Morphogenetic Protein 9 (BMP9) belongs to the Transforming Growth Factor-β (TGF-β) family, which is mainly produced by hepatic stellate cells, which circulates to pulmonary vascular endothelial cells with blood and bind to their receptors to exert corresponding biological effects in PAH, but some research results are contradictory. This article briefly reviews the BMP9 signaling pathway in PAH and the research and progress of BMP9 on pulmonary vascular endothelial cells.
