针对载荷谱时域外推中极值载荷预测的难点——厚尾特征估计,以及极值载荷的确定与识别,系统阐述了区组极大值模型(Block maximum method,BMM)、超阈值模型(Peak over threshold,POT),独立风暴理论模型(Method of independent storms,MI...针对载荷谱时域外推中极值载荷预测的难点——厚尾特征估计,以及极值载荷的确定与识别,系统阐述了区组极大值模型(Block maximum method,BMM)、超阈值模型(Peak over threshold,POT),独立风暴理论模型(Method of independent storms,MIS)3种极值模型的研究进展、数学理论、应用实践及对比分析,得出不同极值模型的优缺点及适用范围,为车辆载荷谱外推和耐久性评估提供了一定的理论参考。展开更多
蛋白质复合体对于研究细胞活动具有重要意义.随着新的生物实验技术的不断出现,产生了大量的蛋白质相互作用网络.通过对蛋白质相互作用网络进行聚类识别蛋白质复合体是当前研究热点.然而,目前大多数蛋白质复合体识别算法的性能不够理想....蛋白质复合体对于研究细胞活动具有重要意义.随着新的生物实验技术的不断出现,产生了大量的蛋白质相互作用网络.通过对蛋白质相互作用网络进行聚类识别蛋白质复合体是当前研究热点.然而,目前大多数蛋白质复合体识别算法的性能不够理想.为此,提出了蛋白质复合体模块度函数(PQ),并在此基础上提出了基于蛋白质复合体模块度函数的模块合并(based on protein complexes modularity function for merging modules,BMM)算法.BMM算法首先识别网络中一些稠密子图作为初始模块,然后依据PQ函数对这些初始模块进行合并,最终得到了质量较高的蛋白质复合体.将识别出的复合体分别与2种已知的蛋白质复合体数据集进行比对,结果表明BMM算法具有很好的识别性能.此外,与其他最新的识别算法相比,BMM算法的识别准确率较高.展开更多
Cyathulae Radix,a traditional Chinese medicine and a common vegetable,boasts a history spanning millennia.It enhances bone density,boosts metabolism,and effectively alleviates osteoporosis-induced pain.Despite its his...Cyathulae Radix,a traditional Chinese medicine and a common vegetable,boasts a history spanning millennia.It enhances bone density,boosts metabolism,and effectively alleviates osteoporosis-induced pain.Despite its historical use,the molecular mechanisms behind Cyathulae Radix’s impact on osteoporosis remain unexplored.In this study,we investigated the effects and mechanisms of Cyathulae Radix ethanol extract(CEE)in inhibiting osteoporosis and osteoclastogenesis.Eight-week-old female mice underwent ovariectomy and were treated with CEE for eight weeks.Micro-computed tomography(micro-CT)assessed histomorphometric parameters,bone tissue staining observed distal femur histomorphology,and three-point bending tests evaluated tibia mechanical properties.Enzyme-linked immunosorbent assay(ELISA)measured serum estradiol(E2),receptor activator for nuclear factor B ligand(RANKL),and osteoprotegerin(OPG)levels.Osteoclastogenesis-related markers were analyzed via Western blotting(WB)and quantitative real-time polymerase chain reaction(qRT-PCR).Additionally,CEE effects on RANKL-induced osteoclast formation and bone resorption were investigated in vitro using tartrate-resistant acid phosphatase(TRAP)staining,qRT-PCR,and WB assay.Compared with the ovariectomy(OVX)group,CEE treatment enhanced trabecular bone density,maximal load-bearing capacity,and various histomorphometric parameters.Serum E2 and OPG levels significantly increased,while Receptor activator of nuclear factor-κB(RANK)decreased in the CEE group.CEE downregulated matrix metallopeptidase 9(MMP-9),Cathepsin K(CTSK),and TRAP gene and protein expression.In bone marrow macrophages(BMMs),CEE reduced mature osteoclasts,bone resorption pit areas,and MMP-9,CTSK,and TRAP expression during osteoclast differentiation.Compared with DMSO treatment,CEE markedly inhibited RANK,TNF receptor associated factor 6(TRAF6),Proto-oncogene c-Fos(c-Fos),Nuclear factor of activated T-cells cytoplasmic 1(NFATc1)expressions,and Extracellular regulated protein kinases(ERK),c-Jun N-terminal kinase(JNK),NF-kappa B-p65(p65)phosphorylation in osteoclasts.In conclusion,CEE significantly inhibits OVX-induced osteoporosis and RANKL-induced osteoclastogenesis,potentially through modulating the Estrogen Receptor(ER)/RANK/NFATc1 signaling pathway.展开更多
文摘针对载荷谱时域外推中极值载荷预测的难点——厚尾特征估计,以及极值载荷的确定与识别,系统阐述了区组极大值模型(Block maximum method,BMM)、超阈值模型(Peak over threshold,POT),独立风暴理论模型(Method of independent storms,MIS)3种极值模型的研究进展、数学理论、应用实践及对比分析,得出不同极值模型的优缺点及适用范围,为车辆载荷谱外推和耐久性评估提供了一定的理论参考。
文摘蛋白质复合体对于研究细胞活动具有重要意义.随着新的生物实验技术的不断出现,产生了大量的蛋白质相互作用网络.通过对蛋白质相互作用网络进行聚类识别蛋白质复合体是当前研究热点.然而,目前大多数蛋白质复合体识别算法的性能不够理想.为此,提出了蛋白质复合体模块度函数(PQ),并在此基础上提出了基于蛋白质复合体模块度函数的模块合并(based on protein complexes modularity function for merging modules,BMM)算法.BMM算法首先识别网络中一些稠密子图作为初始模块,然后依据PQ函数对这些初始模块进行合并,最终得到了质量较高的蛋白质复合体.将识别出的复合体分别与2种已知的蛋白质复合体数据集进行比对,结果表明BMM算法具有很好的识别性能.此外,与其他最新的识别算法相比,BMM算法的识别准确率较高.
基金supported by the National Natural Science Foundation of China(Nos.81273816,81774379,and 81370974)the Natural Science Foundation of Hunan Province,China(Nos.2017JJ2338 and 2020JJ4860)+1 种基金the Fundamental Research Funds for the Central Universities of Central South University(No.502211706)the Fund for the Key Laboratory of Hunan Province,China(No.2017TP1004).
文摘Cyathulae Radix,a traditional Chinese medicine and a common vegetable,boasts a history spanning millennia.It enhances bone density,boosts metabolism,and effectively alleviates osteoporosis-induced pain.Despite its historical use,the molecular mechanisms behind Cyathulae Radix’s impact on osteoporosis remain unexplored.In this study,we investigated the effects and mechanisms of Cyathulae Radix ethanol extract(CEE)in inhibiting osteoporosis and osteoclastogenesis.Eight-week-old female mice underwent ovariectomy and were treated with CEE for eight weeks.Micro-computed tomography(micro-CT)assessed histomorphometric parameters,bone tissue staining observed distal femur histomorphology,and three-point bending tests evaluated tibia mechanical properties.Enzyme-linked immunosorbent assay(ELISA)measured serum estradiol(E2),receptor activator for nuclear factor B ligand(RANKL),and osteoprotegerin(OPG)levels.Osteoclastogenesis-related markers were analyzed via Western blotting(WB)and quantitative real-time polymerase chain reaction(qRT-PCR).Additionally,CEE effects on RANKL-induced osteoclast formation and bone resorption were investigated in vitro using tartrate-resistant acid phosphatase(TRAP)staining,qRT-PCR,and WB assay.Compared with the ovariectomy(OVX)group,CEE treatment enhanced trabecular bone density,maximal load-bearing capacity,and various histomorphometric parameters.Serum E2 and OPG levels significantly increased,while Receptor activator of nuclear factor-κB(RANK)decreased in the CEE group.CEE downregulated matrix metallopeptidase 9(MMP-9),Cathepsin K(CTSK),and TRAP gene and protein expression.In bone marrow macrophages(BMMs),CEE reduced mature osteoclasts,bone resorption pit areas,and MMP-9,CTSK,and TRAP expression during osteoclast differentiation.Compared with DMSO treatment,CEE markedly inhibited RANK,TNF receptor associated factor 6(TRAF6),Proto-oncogene c-Fos(c-Fos),Nuclear factor of activated T-cells cytoplasmic 1(NFATc1)expressions,and Extracellular regulated protein kinases(ERK),c-Jun N-terminal kinase(JNK),NF-kappa B-p65(p65)phosphorylation in osteoclasts.In conclusion,CEE significantly inhibits OVX-induced osteoporosis and RANKL-induced osteoclastogenesis,potentially through modulating the Estrogen Receptor(ER)/RANK/NFATc1 signaling pathway.
