传统空间插值方法可获得福建省区域内降水的总体分布,但该地区气象站点较稀疏且分布不均,导致该区域内降水的空间插值结果误差较大。为提高插值精度,本文利用TRMM卫星数据以弥补站点数据的不足,尝试将TRMM数据作为"软数据"、...传统空间插值方法可获得福建省区域内降水的总体分布,但该地区气象站点较稀疏且分布不均,导致该区域内降水的空间插值结果误差较大。为提高插值精度,本文利用TRMM卫星数据以弥补站点数据的不足,尝试将TRMM数据作为"软数据"、台站数据作为"硬数据",两者相结合后采用贝叶斯最大熵(Bayesian Maximum Entropy,BME)方法对福建省降水的时空格局进行分析。以2000-2012年近13年20个气象站点的年降水量和月降水量为基础数据,分别利用普通克里格法(Ordinary Kriging,OK)和TRMM为"软数据"的BME插值法,分析福建省多年降水的时空分布格局,并对2种方法的插值结果进行比较。结果表明:在时空分布上,以TRMM数据为辅助变量的贝叶斯最大熵插值结果能更好地体现降水的局部差异特征;在误差评价上,以TRMM数据为辅助变量的贝叶斯最大熵插值结果的MAE和RMSE较小,表明TRMM数据作为"软数据"参与插值的BME方法可以在一定程度上弥补站点数据的不足,有效降低预测结果的绝对误差。通过对福建省降水插值的时空分布格局分析和误差评价可看出,BME插值法通过对基础台站数据,以及TRMM卫星产品数据的利用,使降水的时空分析结果更加真实客观,同时,为TRMM卫星降水数据的应用提供了一个新思路。展开更多
从本实验室构建的家蚕蛹cDNA文库中筛选到一条新的cDNA序列,生物信息学分析发现其编码的蛋白质序列与果蝇Enhancer of split基因[E(spl)]编码的DNA结合转录调控因子bHLH(helix-loop-helix)有较高的同源性,将该基因命名为BmE(spl)-like(B...从本实验室构建的家蚕蛹cDNA文库中筛选到一条新的cDNA序列,生物信息学分析发现其编码的蛋白质序列与果蝇Enhancer of split基因[E(spl)]编码的DNA结合转录调控因子bHLH(helix-loop-helix)有较高的同源性,将该基因命名为BmE(spl)-like(Bombyx mori Enhancer of split like)。该基因的ORF长606 bp,编码201个氨基酸残基,预测蛋白质分子质量22.3 kD,等电点9.7。构建重组表达质粒pET-28a-BmE(spl)-like并在E.coli BL21(DE3)菌株中表达BmE(spl)-like蛋白,经亲和层析和FPLC反向柱纯化融合蛋白His-BmE(spl)-like并制备了抗体效价1∶25 600以上的多克隆抗体。用qRT-PCR方法检测BmE(spl)-like在家蚕成虫发育期的转录水平最高,结合果蝇同源基因的功能,推测该基因与蚕蛾的翅发育相关;该基因在家蚕5龄幼虫各组织中的转录水平以表皮最高,马氏管中最低。Western blotting检测BmE(spl)-like在家蚕5龄幼虫性腺中的表达水平最高,其次是丝腺、表皮、脂肪体、肠组织,在头部、马氏管和气管中的表达水平极低,与qRT-PCR检测的BmE(spl)-like在各组织中的转录水平有些差异。展开更多
The preparation of fine copper powders by chemical reduction method was investigated. The reaction of [Cu（NH3）4]2^＋ complex with hydrazine hydrate gives spherical monodispersed fine copper powders. The spherical co...The preparation of fine copper powders by chemical reduction method was investigated. The reaction of [Cu（NH3）4]2^＋ complex with hydrazine hydrate gives spherical monodispersed fine copper powders. The spherical copper powder with a uniform size of 3.5 ± 0.5 μtm was processed to obtain flake copper powder having a uniform size of 8-10 μm, excellent dispersibility and uniform shape. The spherical copper powder of 2.5 ±0.3 μm in size, flake copper, glass frit and vehicle were mixed to prepare copper paste, which was fired in 910-920℃ to obtain BME-MLCC （base metal multilayer ceramic capacitor） with a dense surface of end termination, high adhesion and qualified electrical behavior. Polarized light photo and SEM were employed to observe the copper end termination of BME-MLCC. The rough interface from the interracial reaction between glass and chip gives high adhesion.展开更多
In the article“BME 2.0:Engineering the Future of Medicine”[1],the competing interests statement was inadvertently omitted by the publisher from the published version of the article.This has now been corrected in the...In the article“BME 2.0:Engineering the Future of Medicine”[1],the competing interests statement was inadvertently omitted by the publisher from the published version of the article.This has now been corrected in the PDF and HTML(full text).展开更多
文摘传统空间插值方法可获得福建省区域内降水的总体分布,但该地区气象站点较稀疏且分布不均,导致该区域内降水的空间插值结果误差较大。为提高插值精度,本文利用TRMM卫星数据以弥补站点数据的不足,尝试将TRMM数据作为"软数据"、台站数据作为"硬数据",两者相结合后采用贝叶斯最大熵(Bayesian Maximum Entropy,BME)方法对福建省降水的时空格局进行分析。以2000-2012年近13年20个气象站点的年降水量和月降水量为基础数据,分别利用普通克里格法(Ordinary Kriging,OK)和TRMM为"软数据"的BME插值法,分析福建省多年降水的时空分布格局,并对2种方法的插值结果进行比较。结果表明:在时空分布上,以TRMM数据为辅助变量的贝叶斯最大熵插值结果能更好地体现降水的局部差异特征;在误差评价上,以TRMM数据为辅助变量的贝叶斯最大熵插值结果的MAE和RMSE较小,表明TRMM数据作为"软数据"参与插值的BME方法可以在一定程度上弥补站点数据的不足,有效降低预测结果的绝对误差。通过对福建省降水插值的时空分布格局分析和误差评价可看出,BME插值法通过对基础台站数据,以及TRMM卫星产品数据的利用,使降水的时空分析结果更加真实客观,同时,为TRMM卫星降水数据的应用提供了一个新思路。
文摘从本实验室构建的家蚕蛹cDNA文库中筛选到一条新的cDNA序列,生物信息学分析发现其编码的蛋白质序列与果蝇Enhancer of split基因[E(spl)]编码的DNA结合转录调控因子bHLH(helix-loop-helix)有较高的同源性,将该基因命名为BmE(spl)-like(Bombyx mori Enhancer of split like)。该基因的ORF长606 bp,编码201个氨基酸残基,预测蛋白质分子质量22.3 kD,等电点9.7。构建重组表达质粒pET-28a-BmE(spl)-like并在E.coli BL21(DE3)菌株中表达BmE(spl)-like蛋白,经亲和层析和FPLC反向柱纯化融合蛋白His-BmE(spl)-like并制备了抗体效价1∶25 600以上的多克隆抗体。用qRT-PCR方法检测BmE(spl)-like在家蚕成虫发育期的转录水平最高,结合果蝇同源基因的功能,推测该基因与蚕蛾的翅发育相关;该基因在家蚕5龄幼虫各组织中的转录水平以表皮最高,马氏管中最低。Western blotting检测BmE(spl)-like在家蚕5龄幼虫性腺中的表达水平最高,其次是丝腺、表皮、脂肪体、肠组织,在头部、马氏管和气管中的表达水平极低,与qRT-PCR检测的BmE(spl)-like在各组织中的转录水平有些差异。
文摘The preparation of fine copper powders by chemical reduction method was investigated. The reaction of [Cu（NH3）4]2^＋ complex with hydrazine hydrate gives spherical monodispersed fine copper powders. The spherical copper powder with a uniform size of 3.5 ± 0.5 μtm was processed to obtain flake copper powder having a uniform size of 8-10 μm, excellent dispersibility and uniform shape. The spherical copper powder of 2.5 ±0.3 μm in size, flake copper, glass frit and vehicle were mixed to prepare copper paste, which was fired in 910-920℃ to obtain BME-MLCC （base metal multilayer ceramic capacitor） with a dense surface of end termination, high adhesion and qualified electrical behavior. Polarized light photo and SEM were employed to observe the copper end termination of BME-MLCC. The rough interface from the interracial reaction between glass and chip gives high adhesion.
文摘In the article“BME 2.0:Engineering the Future of Medicine”[1],the competing interests statement was inadvertently omitted by the publisher from the published version of the article.This has now been corrected in the PDF and HTML(full text).
