Bacterial soft rot(BSR)caused by Pectobacterium carotovorum subsp.brasiliense(Pcb)is a serious bacterial disease which negatively impact yield and quality in cucumber.However,the genetic mechanism of BSR resistance in...Bacterial soft rot(BSR)caused by Pectobacterium carotovorum subsp.brasiliense(Pcb)is a serious bacterial disease which negatively impact yield and quality in cucumber.However,the genetic mechanism of BSR resistance in cucumber has not been reported.Here,we investigated the BSR resistance of 119 cucumber core germplasm worldwide at the seedling stage and identified 26 accessions highly resistant to BSR.A total of 1642740 single-nucleotide polymorphisms(SNPs)were used to conduct GWAS,and five loci associated with BSR resistance were detected on four chromosomes:gBSR2.1,gBSR2.2,gBSR3.1,gBSR4.1 and gBSR5.1.Based on haplotype analysis,sequence polymorphisms,functional annotation and qRT-PCR analysis,six candidate genes were identified within the five loci.CsaV3_2G014450,CsaV3_2G014490,CsaV3_2G016000,CsaV3_3G000850,CsaV3_4G033150,and CsaV3_5G000390 each had nonsynonymous SNPs,and were significantly up-regulated in the resistant genotypes after inoculation.And CsaV3_5G000390 in the susceptible genotype was significantly up-regulated after inoculation.The identification of these candidate genes lays a foundation for understanding the genetic mechanism of BSR resistance in cucumber.Generally,our study mined genes associated with BSR resistance in cucumber seedlings and will assist the breeding of BSR-resistant cucumber cultivars.展开更多
采用最优线性无偏估计(Best Linear Unbiased Prediction,BLUP)方法对甘蔗亲本的单性状育种值进行估计,目的在于为亲本的正确选择与科学组配提供重要的理论基础。对2010年YT系列的4个母性亲本21个杂交组合的实生苗后代单丛产量的单性状...采用最优线性无偏估计(Best Linear Unbiased Prediction,BLUP)方法对甘蔗亲本的单性状育种值进行估计,目的在于为亲本的正确选择与科学组配提供重要的理论基础。对2010年YT系列的4个母性亲本21个杂交组合的实生苗后代单丛产量的单性状育种值进行计算,结果发现,4个母本的优劣顺序为YT00-236>YT01-125>YT00-319>YT83-257,而3个区组效应差异不大。建议结合甘蔗生产实际,进一步探索适宜的亲本评价与选择方法。展开更多
基金supported by the National Key Research and Development Program of China(Grant No.2023YFD1200101)the Earmarked Fund for Modern Agro-industry Technology Research System(Grant No.CARS-23)Science and Technology Innovation Program of the Chinese Academy of Agricultural Science(Grant No.CAAS-ASTIP-IVFCAAS).
文摘Bacterial soft rot(BSR)caused by Pectobacterium carotovorum subsp.brasiliense(Pcb)is a serious bacterial disease which negatively impact yield and quality in cucumber.However,the genetic mechanism of BSR resistance in cucumber has not been reported.Here,we investigated the BSR resistance of 119 cucumber core germplasm worldwide at the seedling stage and identified 26 accessions highly resistant to BSR.A total of 1642740 single-nucleotide polymorphisms(SNPs)were used to conduct GWAS,and five loci associated with BSR resistance were detected on four chromosomes:gBSR2.1,gBSR2.2,gBSR3.1,gBSR4.1 and gBSR5.1.Based on haplotype analysis,sequence polymorphisms,functional annotation and qRT-PCR analysis,six candidate genes were identified within the five loci.CsaV3_2G014450,CsaV3_2G014490,CsaV3_2G016000,CsaV3_3G000850,CsaV3_4G033150,and CsaV3_5G000390 each had nonsynonymous SNPs,and were significantly up-regulated in the resistant genotypes after inoculation.And CsaV3_5G000390 in the susceptible genotype was significantly up-regulated after inoculation.The identification of these candidate genes lays a foundation for understanding the genetic mechanism of BSR resistance in cucumber.Generally,our study mined genes associated with BSR resistance in cucumber seedlings and will assist the breeding of BSR-resistant cucumber cultivars.
文摘采用最优线性无偏估计(Best Linear Unbiased Prediction,BLUP)方法对甘蔗亲本的单性状育种值进行估计,目的在于为亲本的正确选择与科学组配提供重要的理论基础。对2010年YT系列的4个母性亲本21个杂交组合的实生苗后代单丛产量的单性状育种值进行计算,结果发现,4个母本的优劣顺序为YT00-236>YT01-125>YT00-319>YT83-257,而3个区组效应差异不大。建议结合甘蔗生产实际,进一步探索适宜的亲本评价与选择方法。
