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BLOC-3介导Rab32线粒体定位改变对肝癌细胞生长的作用研究 被引量:1
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作者 刘小丽 廖达文 +7 位作者 王星琛 许小君 魏元元 王楠 张志刚 孙夏承 黄启超 季乐乐 《中国癌症防治杂志》 CAS 2023年第2期129-137,共9页
目的观察溶酶体相关细胞器生物发生复合体-3(biogenesis of lysosome-related organelles complex-3,BLOC-3)亚基Hps1和Hps4对肝癌细胞中Rab32线粒体定位的影响及在肝癌细胞生长中的作用。方法通过公共数据库GenDoma,String和InBio Disc... 目的观察溶酶体相关细胞器生物发生复合体-3(biogenesis of lysosome-related organelles complex-3,BLOC-3)亚基Hps1和Hps4对肝癌细胞中Rab32线粒体定位的影响及在肝癌细胞生长中的作用。方法通过公共数据库GenDoma,String和InBio Discover分析Hps1和Hps4与Rab32的相互作用情况。体外培养人肝癌细胞系SNU-739和Hep-3B,利用脂质体转染方法分别转染Hps1和Hps4相关的siRNAs和质粒,采用免疫荧光和Western blot观察Hps1和Hps4对Rab32线粒体定位的影响,细胞划痕、克隆形成、EdU、MTS及Transwell侵袭实验检测Hps1和Hps4调控Rab32线粒体定位后肝癌细胞迁移、增殖和侵袭的变化情况。通过公共数据库UALCAN中的数据集CPTAC分析Rab32蛋白在肝癌和正常肝脏组织中的表达差异。结果数据库分析结果显示,Hps1和Hps4均可以与Rab32相互作用;与正常肝脏组织相比,Rab32蛋白在肝癌组织中的表达显著降低(P<0.001)。在肝癌细胞系SNU-739中干涉Hps1或Hps4或同时干涉Hps1和Hps4后,Rab32线粒体定位均减少(均P<0.01),细胞线粒体Rab32蛋白表达均降低(均P<0.001),细胞增殖、迁移和侵袭能力增强(均P<0.05);在肝癌细胞系Hep-3B中过表达Hps1和Hps4后,Rab32线粒体定位增多(P<0.01),细胞线粒体Rab32蛋白表达增加(P<0.001),细胞增殖、迁移和侵袭能力均受抑制(均P<0.01),而单独过表达Hps1或Hps4时无显著抑制作用(均P>0.05)。结论BLOC-3亚基Hps1和Hps4均可与Rab32相互作用并增加Rab32线粒体定位,进而抑制肝癌细胞生长。 展开更多
关键词 肝癌 bloc-3 Rab32 线粒体 迁移 侵袭
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RMB Internationalization and Its Implications for Financial and Monetary Cooperation in East Asia 被引量:35
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作者 Yung Ckul Park 《China & World Economy》 SCIE 2010年第2期1-21,共21页
This paper emphasizes the importance of the denomination of financial assets rather than trade invoicing as a long-term determinant of an international currency. China needs to liberalize and open its financial system... This paper emphasizes the importance of the denomination of financial assets rather than trade invoicing as a long-term determinant of an international currency. China needs to liberalize and open its financial system and make the RMB fully convertible, and to adopt a moreflexible exchange rate system to speed up its currency internationalization, for which China could follow either a global or regional approach. The global approach is a riskier and more unpredictable strategy because it would demand a scope and timeframe of financial liberalization that is likely to be beyond China's institutional capacity over the coming decade. There are two options that could be taken in following the regional approach. One is creating an ASEAN+ New 3 (the Chinese Mainland, Hong Kong, and Taiwan) RMB bloc and the other is liberalizing China's financial industries and internationalizing the RMB by playing a leading role in East Asia's economic integration within the framework of ASEAN+ 3, This paper concludes that the latter is a more realistic and effective approach for China. 展开更多
关键词 ASEAN+New 3 ASEAN+3 financial liberalization RMB bloc RMB internationalization
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