Objective:To observe the effect of the early intervention with moxibustion at "Shènshū(肾俞BL23)" on learning and memory of Alzheimer’s disease(AD) rats modeled by ovariectomy and D-Galactose(D-Gal)in...Objective:To observe the effect of the early intervention with moxibustion at "Shènshū(肾俞BL23)" on learning and memory of Alzheimer’s disease(AD) rats modeled by ovariectomy and D-Galactose(D-Gal)injection and the effect on Ca2+/CaMKII/CREB signaling pathway so as to provide the evidence for the acupoint selection in the early intervention with acupuncture and moxibustion for AD.Methods:Sixty female SD rats at the age of 3 months were selected and randomly divided into a normal group,a sham-operation group,a model group,a "BL23" group,a "Wèishū"(胃俞BL21) group,and a non-acupoint group,10 rats in each group.Except in the normal group and the sham-operation group,bilateral ovaries were removed in the other 4 groups.3 days after the operation,D-Gal was injected intraperitoneally at a dose of 150 mg/kg/d for 90 days to establish AD rat model.In the sham-operation group,only the peripheral fat of ovarian was removed and 0.9% normal saline was injected intraperitoneally of the same dose for 90 days.In the "BL23" group,the "BL21" group and the non-acupoints group,since the 2 nd day after ovariectomy,moxibustion was applied at 9:00 a.m.every day in the rats.The rats were fixed on a fixed table,the mild moxibustion with self-prepared moxa stick was applied to bilateral "BL23""BL21" and the non-acupoint of each rat,the ignited end of moxa stick was 2 cm to 3 cm far from skin surface.The infrared thermometer was adopted to measure the temperature of the epidermis of each acupoint from time to time so as to maintain the local temperature to be(41 ± 0.5)℃.Mild moxibustion lasted for 10 min each time,consecutively for 5 days a week and at an interval of 2 days.The total consecutive intervention duration was 12 weeks.In the normal group and the shamoperation group,no moxibustion intervention was performed,but the rats were restricted in a same way during the experiment.At the end of modeling and intervention,water maze test was adopted to detect the learning and memory abilities of rats in each group.The western blotting was used to detect the expressions of p-CREB,p-CaMKII,CaMKII and CREB in hippocampus of rats in each group.Results:(1)Navigation test:since Day 72,compared with the sham-operation group,the escape latencies in all of the consecutive 5 days were longer in the model group(all P <0.05).Since Day 73,compared with the normal group,the escape latency in all of the consecutive 4 days was shorter in the "BL23"group(all P <0.05).(2) Times across platform:compared with the normal group,the number of times consecutively crossing the platform was lower in the model group(P <0.05).After moxibustion,compared with the model group,the number of times across platform was higher in the "BL23"group,indicating a statistical significance(P <0.05).(3)Moving track of water maze spatial probe:the "tendency"strategy was displayed in the normal group and the sham operation group.The "marginal" strategy,which is commonly seen in the AD rats,was displayed in the model group.The "random" strategy was displayed in the "BL23" group.The "marginal" strategy was presented in the "BL21" group and the non-acupoint group.(4) Expressions of hippocampal P-CAMKII,CAMKII,CREB and P-CREB in the rats:compared with the normal group,the phosphorylation degrees of CAMKII and CREB were significantly reduced(both P<0.05) in the model group.In 12 weeks of moxibustion,compared with the model group,the levels of P-CAMKII and P-CREB were increased in the "BL23" group and the "BL21" group(all P <0.05) and the levels in the "BL23" group were higher than that of the "BL21" group and the non-acupoint group(all P<0.05).Conclusion:Moxibustion at "BL23" achieves a better effect in improving the learning and memory of AD rats modeled by ovariectomy and D-Gal injection as compared with moxibustion at "BL21" and the non-acupoint.The effect mechanism is potentially related to the effective activation of Ca2+/CaMKII/CREB signaling pathway.展开更多
OBJECTIVE:To examine whether specific stimulation of Shenshu(BL23)affects sympathetic nervous activity(SNA)-associated plasma renin concentration(PRC).METHODS:Eight healthy volunteers participated in three pattern con...OBJECTIVE:To examine whether specific stimulation of Shenshu(BL23)affects sympathetic nervous activity(SNA)-associated plasma renin concentration(PRC).METHODS:Eight healthy volunteers participated in three pattern conditions in random order:control(Cont),stimulation of Shenshu(BL23),and stimulation of sham point(Sham).All participants were initially in the supine position for>60 min,and then remained in the standing position during the experimental procedure to increase SNA.An electrocardiogram was used to calculate low frequency/high frequency(LF/HF)ratio;blood was collected to analyze PRC.RESULTS:The LF/HF ratio was significantly increased in the standing position when compared with the supine position(P<0.01).There was no difference in LF/HF ratio during or after stimulation of Shenshu(BL23)in the standing position when compared with before the stimulation in the supine position;however,the LF/HF ratio was significantly increased in Cont and Sham conditions(P<0.01).There was no difference in PRC after stimulation of Shenshu(BL23)in the standing position when compared with before the stimulation in the supine position;however,there was a significant increase in PRC in the Cont and Sham conditions(Cont P<0.05,Sham P<0.01).CONCLUSION:Our results demonstrated that specific acupuncture stimulation of Shenshu(BL23)in the standing position decreased SNA-associated PRC,which was not observed during acupuncture stimulation of the sham point.展开更多
OBJECTIVE： It is unknown whether the psoas major muscle, thought to be a key muscle for treatment of lower back pain, can be punctured at Shenshu（BL23）.METHODS： Twelve dissected specimens were used for studying th...OBJECTIVE： It is unknown whether the psoas major muscle, thought to be a key muscle for treatment of lower back pain, can be punctured at Shenshu（BL23）.METHODS： Twelve dissected specimens were used for studying the needling pathway of BL23 by perpendicularly inserting the depth-measuring blade of a vernier caliper at BL23. Dimensions of psoas muscle were measured. Correlation studies were conducted. In addition, our samples were grouped by gender and underlying medical conditions for analysis.RESULTS： Half（50%） of the needle insertions successfully punctured psoas muscle. The mean depth of needle insertion to puncture psoas muscle（D_（min）） in the group with short-term underlying medical conditions was 38.0 mm（interquartile range 29.0–51.8 mm）, approximately 6 mm deeper than 32.0（29.3–42.5） mm in the group with long-term health problems（P = 0.041）. The cross-sectional area（CSA） of psoas muscle in the former group was on average approximately 1.5 times that of the latter group（P = 0.04）. When the data were analysed by gender, the thickness of psoas muscle in the male group was 19.0（6.5–24.0） mm compared to 19.5（5.8–34.8） mm in the female group（P = 0.02）. The age in the female group（P = 0.04） and the body length of the total group（P = 0.04） negatively correlated to D_（min）.CONCLUSION： Needle insertion at BL23 might be able to puncture psoas muscle. Differences in the CSA of psoas muscle and D_（min） were observed in groups with short-term and long-term underlying medical conditions.展开更多
Objective Sepsis,a life-threatening condition caused by a dysregulated host response to infection,continues to be a major cause of mortality in critical care despite medical advancements.This study aimed to investigat...Objective Sepsis,a life-threatening condition caused by a dysregulated host response to infection,continues to be a major cause of mortality in critical care despite medical advancements.This study aimed to investigate the therapeutic effects and neuroimmune mechanisms of electroacupuncture(EA)at the Shenshu(BL23)acupoint combined with antibiotic therapy in sepsis management.Methods A cecal ligation and puncture(CLP)-induced murine sepsis model was used to evaluate the combined therapy.The study employed enzyme-linked immunosorbent assays(ELISA)and histological analysis to assess systemic inflammation and intestinal damage.Three-dimensional immunolabeling of c-Fos neurons and chemogenetic modulation of hypothalamic paraventricular nucleus(PVN)neurons were performed to investigate neural mechanisms.Additionally,serum catecholamine levels were measured using ultra-performance liquid chromatography tandem mass spectrometry(UPLC-MS/MS).Results The combination of EA and antibiotics significantly improved survival rates and reduced sepsis-induced weight loss.The therapy lowered systemic levels of proinflammatory cytokines(TNF-αand IL-6)and mitigated intestinal inflammatory infiltration.EA at BL23 suppressed neuronal activation in the PVN by reducing c-Fos expression.Chemogenetic inhibition of corticotropin-releasing hormone(CRH)neurons replicated the anti-inflammatory effects of the therapy,while their activation diminished therapeutic benefits.Conversely,activation of oxytocin(OXT)neurons reproduced the anti-inflammatory effects,and their inhibition reversed these benefits.The combined therapy also elevated serum catecholamine levels,indicating sympathetic-mediated immunomodulation.Conclusion This study demonstrates that EA at BL23 enhances antibiotic efficacy in sepsis by modulating PVN activity—suppressing CRH neurons and activating OXT neurons—leading to increased catecholamine secretion and systemic inflammation control.These findings reveal a novel neuroimmunological pathway for acupuncture’s therapeutic role in sepsis,supporting its potential as a noninvasive adjunctive therapy in critical care.展开更多
Objective:To investigate the distribution characteristics of sensory,sympathetic,and motor neurons as-sociated with the"Shenshu(BL 23)"and"Guanyuan(CV 4)"acupoints and the ovary,and to elucidate th...Objective:To investigate the distribution characteristics of sensory,sympathetic,and motor neurons as-sociated with the"Shenshu(BL 23)"and"Guanyuan(CV 4)"acupoints and the ovary,and to elucidate the neural anatomical connections between these acupoints and the ovary.Methods:In this study,dual fluorescent neural tracing was utilized,with twelve female Sprague-Dawley rats being randomly allocated into two groups(n=6 per group)for the purpose of administering tracer injections at distinct anatomical sites:the"BL 23"-ovary group received injections of Alexa Fluor 488-conjugated cholera toxin subunit B(AF488-CTB)at the left"BL 23"and Fluoro-Gold(FG)in the left ovary;the"CV 4"-ovary group received AF488-CTB at“CV 4”and FG in the left ovary.Three days post-injection,the distribution of AF488-CTB and FG-labeled neural components was observed in the dorsal root ganglia(DRGs),sympathetic chain,and spinal cord tissues.Results:①Sensory neurons labeled with AF488-CTB associated with"BL 23"were primarily distributed in the thoracic(T)10 to lumbar(L)2 DRGs,concentrated in T12-T13;postganglionic sympathetic neurons were mainly located in the thoracolumbar sympathetic chain;motor neurons were predominantly found in the anterior horn of the T11-L1 spinal cord segments.②Sensory neurons labeled with AF488-CTB associated with"CV 4"were primarily located in T10-L3 DRGs,concentrated in T13-L2 DRGs;postgan-glionic sympathetic neurons were mainly distributed in the lumbar sympathetic chain;motor neurons were primarily found in the anterior horn of T11-L2 spinal cord segments.③FG-labeled sensory neu-rons associated with the ovary were mainly distributed in the T9-L3 DRGs,concentrated in the T12-L2 DRGs;postganglionic sympathetic neurons were primarily located in the lumbar sympathetic chain.④Double-labeled(AF488-CTB and FG)sensory neurons associated with the"BL 23"and ovary were mainly distributed in the T12-T13 DRGs,with double-labeled postganglionic sympathetic neurons primarily in the lumbar sympathetic chain;double-labeled sensory neurons associated with the"CV 4"and ovary were mainly distributed in the T13-L1 DRGs,with double-labeled postganglionic sympathetic neurons primar-ily in the lumbar sympathetic chain.No double-labeled motor neurons were observed for either acupoint with the ovary.Conclusion:"BL 23"and"CV 4"and the ovary in rats exhibit segmental connections in sensory and sympathetic innervation,which may serve as the neural anatomical pathway and basis for acupuncture at these acupoints to regulate ovarian function.展开更多
目的观察电针委中穴对腰部多裂肌损伤后的干预作用及对胰岛素样生长因子1(insulin like growth factor 1,IGF-1)表达的影响,探求委中穴对大鼠腰肌损伤后再生修复的可能机制。方法雄性SD大鼠120只,随机分为空白组、模型对照组、模型组、...目的观察电针委中穴对腰部多裂肌损伤后的干预作用及对胰岛素样生长因子1(insulin like growth factor 1,IGF-1)表达的影响,探求委中穴对大鼠腰肌损伤后再生修复的可能机制。方法雄性SD大鼠120只,随机分为空白组、模型对照组、模型组、电针委中组、电针肾俞组,共5组,观察3个时间点(4天、7天、14天)的变化,每个时间点8个样本。将0.5%布比卡因盐酸盐溶液按每点100μL注射于模型组和电针组大鼠L4、L5水平的多裂肌上。模型对照组采用同样方法注射生理盐水,空白组不做处理。造模后进行电针双侧委中穴或肾俞穴分别治疗4天、7天、14天,HE染色观察肌细胞形态学的改变,免疫组化方法检测肌细胞IGF-1的表达。结果造模前后多裂肌形态学改变显著,损伤后第14天仍未完全恢复。电针委中组与电针肾俞组从形态学上优于模型组。第4、7天模型组多裂肌IGF-1的表达显著高于空白组(P<0.01);第4天电针委中组表达显著高于模型组(P<0.01),电针委中组表达高于电针肾俞组(P<0.05),电针肾俞组表达高于模型组(P<0.05),而在第14天,电针肾俞组表达显著高于模型组与电针委中组(P<0.01)。结论电针委中穴和电针肾俞穴均能够促进大鼠腰多裂肌损伤后的再生,电针委中穴在肌肉损伤的早期效果显著。展开更多
基金Supported by National Natural Science Foundation of China Project:No.81473786No.81873380。
文摘Objective:To observe the effect of the early intervention with moxibustion at "Shènshū(肾俞BL23)" on learning and memory of Alzheimer’s disease(AD) rats modeled by ovariectomy and D-Galactose(D-Gal)injection and the effect on Ca2+/CaMKII/CREB signaling pathway so as to provide the evidence for the acupoint selection in the early intervention with acupuncture and moxibustion for AD.Methods:Sixty female SD rats at the age of 3 months were selected and randomly divided into a normal group,a sham-operation group,a model group,a "BL23" group,a "Wèishū"(胃俞BL21) group,and a non-acupoint group,10 rats in each group.Except in the normal group and the sham-operation group,bilateral ovaries were removed in the other 4 groups.3 days after the operation,D-Gal was injected intraperitoneally at a dose of 150 mg/kg/d for 90 days to establish AD rat model.In the sham-operation group,only the peripheral fat of ovarian was removed and 0.9% normal saline was injected intraperitoneally of the same dose for 90 days.In the "BL23" group,the "BL21" group and the non-acupoints group,since the 2 nd day after ovariectomy,moxibustion was applied at 9:00 a.m.every day in the rats.The rats were fixed on a fixed table,the mild moxibustion with self-prepared moxa stick was applied to bilateral "BL23""BL21" and the non-acupoint of each rat,the ignited end of moxa stick was 2 cm to 3 cm far from skin surface.The infrared thermometer was adopted to measure the temperature of the epidermis of each acupoint from time to time so as to maintain the local temperature to be(41 ± 0.5)℃.Mild moxibustion lasted for 10 min each time,consecutively for 5 days a week and at an interval of 2 days.The total consecutive intervention duration was 12 weeks.In the normal group and the shamoperation group,no moxibustion intervention was performed,but the rats were restricted in a same way during the experiment.At the end of modeling and intervention,water maze test was adopted to detect the learning and memory abilities of rats in each group.The western blotting was used to detect the expressions of p-CREB,p-CaMKII,CaMKII and CREB in hippocampus of rats in each group.Results:(1)Navigation test:since Day 72,compared with the sham-operation group,the escape latencies in all of the consecutive 5 days were longer in the model group(all P <0.05).Since Day 73,compared with the normal group,the escape latency in all of the consecutive 4 days was shorter in the "BL23"group(all P <0.05).(2) Times across platform:compared with the normal group,the number of times consecutively crossing the platform was lower in the model group(P <0.05).After moxibustion,compared with the model group,the number of times across platform was higher in the "BL23"group,indicating a statistical significance(P <0.05).(3)Moving track of water maze spatial probe:the "tendency"strategy was displayed in the normal group and the sham operation group.The "marginal" strategy,which is commonly seen in the AD rats,was displayed in the model group.The "random" strategy was displayed in the "BL23" group.The "marginal" strategy was presented in the "BL21" group and the non-acupoint group.(4) Expressions of hippocampal P-CAMKII,CAMKII,CREB and P-CREB in the rats:compared with the normal group,the phosphorylation degrees of CAMKII and CREB were significantly reduced(both P<0.05) in the model group.In 12 weeks of moxibustion,compared with the model group,the levels of P-CAMKII and P-CREB were increased in the "BL23" group and the "BL21" group(all P <0.05) and the levels in the "BL23" group were higher than that of the "BL21" group and the non-acupoint group(all P<0.05).Conclusion:Moxibustion at "BL23" achieves a better effect in improving the learning and memory of AD rats modeled by ovariectomy and D-Gal injection as compared with moxibustion at "BL21" and the non-acupoint.The effect mechanism is potentially related to the effective activation of Ca2+/CaMKII/CREB signaling pathway.
文摘OBJECTIVE:To examine whether specific stimulation of Shenshu(BL23)affects sympathetic nervous activity(SNA)-associated plasma renin concentration(PRC).METHODS:Eight healthy volunteers participated in three pattern conditions in random order:control(Cont),stimulation of Shenshu(BL23),and stimulation of sham point(Sham).All participants were initially in the supine position for>60 min,and then remained in the standing position during the experimental procedure to increase SNA.An electrocardiogram was used to calculate low frequency/high frequency(LF/HF)ratio;blood was collected to analyze PRC.RESULTS:The LF/HF ratio was significantly increased in the standing position when compared with the supine position(P<0.01).There was no difference in LF/HF ratio during or after stimulation of Shenshu(BL23)in the standing position when compared with before the stimulation in the supine position;however,the LF/HF ratio was significantly increased in Cont and Sham conditions(P<0.01).There was no difference in PRC after stimulation of Shenshu(BL23)in the standing position when compared with before the stimulation in the supine position;however,there was a significant increase in PRC in the Cont and Sham conditions(Cont P<0.05,Sham P<0.01).CONCLUSION:Our results demonstrated that specific acupuncture stimulation of Shenshu(BL23)in the standing position decreased SNA-associated PRC,which was not observed during acupuncture stimulation of the sham point.
文摘OBJECTIVE： It is unknown whether the psoas major muscle, thought to be a key muscle for treatment of lower back pain, can be punctured at Shenshu（BL23）.METHODS： Twelve dissected specimens were used for studying the needling pathway of BL23 by perpendicularly inserting the depth-measuring blade of a vernier caliper at BL23. Dimensions of psoas muscle were measured. Correlation studies were conducted. In addition, our samples were grouped by gender and underlying medical conditions for analysis.RESULTS： Half（50%） of the needle insertions successfully punctured psoas muscle. The mean depth of needle insertion to puncture psoas muscle（D_（min）） in the group with short-term underlying medical conditions was 38.0 mm（interquartile range 29.0–51.8 mm）, approximately 6 mm deeper than 32.0（29.3–42.5） mm in the group with long-term health problems（P = 0.041）. The cross-sectional area（CSA） of psoas muscle in the former group was on average approximately 1.5 times that of the latter group（P = 0.04）. When the data were analysed by gender, the thickness of psoas muscle in the male group was 19.0（6.5–24.0） mm compared to 19.5（5.8–34.8） mm in the female group（P = 0.02）. The age in the female group（P = 0.04） and the body length of the total group（P = 0.04） negatively correlated to D_（min）.CONCLUSION： Needle insertion at BL23 might be able to puncture psoas muscle. Differences in the CSA of psoas muscle and D_（min） were observed in groups with short-term and long-term underlying medical conditions.
基金the National Key Research and Development Program of China(2022YFC3500704).
文摘Objective Sepsis,a life-threatening condition caused by a dysregulated host response to infection,continues to be a major cause of mortality in critical care despite medical advancements.This study aimed to investigate the therapeutic effects and neuroimmune mechanisms of electroacupuncture(EA)at the Shenshu(BL23)acupoint combined with antibiotic therapy in sepsis management.Methods A cecal ligation and puncture(CLP)-induced murine sepsis model was used to evaluate the combined therapy.The study employed enzyme-linked immunosorbent assays(ELISA)and histological analysis to assess systemic inflammation and intestinal damage.Three-dimensional immunolabeling of c-Fos neurons and chemogenetic modulation of hypothalamic paraventricular nucleus(PVN)neurons were performed to investigate neural mechanisms.Additionally,serum catecholamine levels were measured using ultra-performance liquid chromatography tandem mass spectrometry(UPLC-MS/MS).Results The combination of EA and antibiotics significantly improved survival rates and reduced sepsis-induced weight loss.The therapy lowered systemic levels of proinflammatory cytokines(TNF-αand IL-6)and mitigated intestinal inflammatory infiltration.EA at BL23 suppressed neuronal activation in the PVN by reducing c-Fos expression.Chemogenetic inhibition of corticotropin-releasing hormone(CRH)neurons replicated the anti-inflammatory effects of the therapy,while their activation diminished therapeutic benefits.Conversely,activation of oxytocin(OXT)neurons reproduced the anti-inflammatory effects,and their inhibition reversed these benefits.The combined therapy also elevated serum catecholamine levels,indicating sympathetic-mediated immunomodulation.Conclusion This study demonstrates that EA at BL23 enhances antibiotic efficacy in sepsis by modulating PVN activity—suppressing CRH neurons and activating OXT neurons—leading to increased catecholamine secretion and systemic inflammation control.These findings reveal a novel neuroimmunological pathway for acupuncture’s therapeutic role in sepsis,supporting its potential as a noninvasive adjunctive therapy in critical care.
基金Supported by the National Natural Science Foundation of China:82305409National Key Research and Development Program of China:2022YFC3500504。
文摘Objective:To investigate the distribution characteristics of sensory,sympathetic,and motor neurons as-sociated with the"Shenshu(BL 23)"and"Guanyuan(CV 4)"acupoints and the ovary,and to elucidate the neural anatomical connections between these acupoints and the ovary.Methods:In this study,dual fluorescent neural tracing was utilized,with twelve female Sprague-Dawley rats being randomly allocated into two groups(n=6 per group)for the purpose of administering tracer injections at distinct anatomical sites:the"BL 23"-ovary group received injections of Alexa Fluor 488-conjugated cholera toxin subunit B(AF488-CTB)at the left"BL 23"and Fluoro-Gold(FG)in the left ovary;the"CV 4"-ovary group received AF488-CTB at“CV 4”and FG in the left ovary.Three days post-injection,the distribution of AF488-CTB and FG-labeled neural components was observed in the dorsal root ganglia(DRGs),sympathetic chain,and spinal cord tissues.Results:①Sensory neurons labeled with AF488-CTB associated with"BL 23"were primarily distributed in the thoracic(T)10 to lumbar(L)2 DRGs,concentrated in T12-T13;postganglionic sympathetic neurons were mainly located in the thoracolumbar sympathetic chain;motor neurons were predominantly found in the anterior horn of the T11-L1 spinal cord segments.②Sensory neurons labeled with AF488-CTB associated with"CV 4"were primarily located in T10-L3 DRGs,concentrated in T13-L2 DRGs;postgan-glionic sympathetic neurons were mainly distributed in the lumbar sympathetic chain;motor neurons were primarily found in the anterior horn of T11-L2 spinal cord segments.③FG-labeled sensory neu-rons associated with the ovary were mainly distributed in the T9-L3 DRGs,concentrated in the T12-L2 DRGs;postganglionic sympathetic neurons were primarily located in the lumbar sympathetic chain.④Double-labeled(AF488-CTB and FG)sensory neurons associated with the"BL 23"and ovary were mainly distributed in the T12-T13 DRGs,with double-labeled postganglionic sympathetic neurons primarily in the lumbar sympathetic chain;double-labeled sensory neurons associated with the"CV 4"and ovary were mainly distributed in the T13-L1 DRGs,with double-labeled postganglionic sympathetic neurons primar-ily in the lumbar sympathetic chain.No double-labeled motor neurons were observed for either acupoint with the ovary.Conclusion:"BL 23"and"CV 4"and the ovary in rats exhibit segmental connections in sensory and sympathetic innervation,which may serve as the neural anatomical pathway and basis for acupuncture at these acupoints to regulate ovarian function.
文摘目的观察电针委中穴对腰部多裂肌损伤后的干预作用及对胰岛素样生长因子1(insulin like growth factor 1,IGF-1)表达的影响,探求委中穴对大鼠腰肌损伤后再生修复的可能机制。方法雄性SD大鼠120只,随机分为空白组、模型对照组、模型组、电针委中组、电针肾俞组,共5组,观察3个时间点(4天、7天、14天)的变化,每个时间点8个样本。将0.5%布比卡因盐酸盐溶液按每点100μL注射于模型组和电针组大鼠L4、L5水平的多裂肌上。模型对照组采用同样方法注射生理盐水,空白组不做处理。造模后进行电针双侧委中穴或肾俞穴分别治疗4天、7天、14天,HE染色观察肌细胞形态学的改变,免疫组化方法检测肌细胞IGF-1的表达。结果造模前后多裂肌形态学改变显著,损伤后第14天仍未完全恢复。电针委中组与电针肾俞组从形态学上优于模型组。第4、7天模型组多裂肌IGF-1的表达显著高于空白组(P<0.01);第4天电针委中组表达显著高于模型组(P<0.01),电针委中组表达高于电针肾俞组(P<0.05),电针肾俞组表达高于模型组(P<0.05),而在第14天,电针肾俞组表达显著高于模型组与电针委中组(P<0.01)。结论电针委中穴和电针肾俞穴均能够促进大鼠腰多裂肌损伤后的再生,电针委中穴在肌肉损伤的早期效果显著。
