6G is desired to support more intelligence networks and this trend attaches importance to the self-healing capability if degradation emerges in the cellular networks.As a primary component of selfhealing networks,faul...6G is desired to support more intelligence networks and this trend attaches importance to the self-healing capability if degradation emerges in the cellular networks.As a primary component of selfhealing networks,fault detection is investigated in this paper.Considering the fast response and low timeand-computational consumption,it is the first time that the Online Broad Learning System(OBLS)is applied to identify outages in cellular networks.In addition,the Automatic-constructed Online Broad Learning System(AOBLS)is put forward to rationalize its structure and consequently avoid over-fitting and under-fitting.Furthermore,a multi-layer classification structure is proposed to further improve the classification performance.To face the challenges caused by imbalanced data in fault detection problems,a novel weighting strategy is derived to achieve the Multilayer Automatic-constructed Weighted Online Broad Learning System(MAWOBLS)and ensemble learning with retrained Support Vector Machine(SVM),denoted as EMAWOBLS,for superior treatment with this imbalance issue.Simulation results show that the proposed algorithm has excellent performance in detecting faults with satisfactory time usage.展开更多
Objective This study aimed to comprehensively investigate the potential protective effects and underlying mechanisms of taurine against dihydrotestosterone(DHT)-induced androgenetic alopecia(AGA)in male C57BL/6 mice,w...Objective This study aimed to comprehensively investigate the potential protective effects and underlying mechanisms of taurine against dihydrotestosterone(DHT)-induced androgenetic alopecia(AGA)in male C57BL/6 mice,with a focus on hair follicle cycle modulation,cellular proliferation/apoptosis,and key related signaling pathways.Methods Six-week-old female C57BL/6 mice were initially used to assess the hair growth-promoting potential of taurine.After acclimatization,they were randomly assigned to three groups(n=8):control(regular drinking water),taurine(drinking water containing 1%taurine),and minoxidil(topical 2%minoxidil,positive control).For the AGA study,male C57BL/6 mice were randomly divided into five groups(n=8):control(physiological saline),DHT(model group,1 mg/d DHT),DHT+low-dose taurine(1 mg/d DHT+2 mg/d taurine),DHT+high-dose taurine(1 mg/d DHT+10 mg/d taurine),and DHT+minoxidil(positive control,1 mg/d DHT+topical 2%minoxidil).One day before treatment initiation,dorsal hair was shaved with scissors,and residual hair was removed using a depilatory cream.DHT and taurine were administered via daily intraperitoneal injection.Hair regrowth was assessed by photographing the depilated area at regular intervals and quantified using a four-point grading system(0-3).Dorsal skin samples were collected on day 14 for histological analysis(H&E staining),immunofluorescence staining(Ki67 for proliferation,TUNEL for apoptosis),ELISA(DHT quantification),RT-qPCR,and Western blot analysis to evaluate the expression of key genes and proteins(androgen receptor(AR),transforming growth factor(TGF)‑β1,TGF‑β2,Dickkopf-1(DKK1)).Results In female mice,taurine supplementation significantly accelerated hair growth,with effects comparable to minoxidil.This was evidenced by an earlier transition from pink(telogen)to black(anagen)skin and increased hair growth scores.Histological analysis showed that taurine increased hair follicle count and dermal thickness.Immunofluorescence confirmed enhanced keratinocyte proliferation in the hair matrix.In the DHTinduced AGA model,DHT significantly extended the telogen phase,inhibited hair growth,increased skin DHT content,and induced hair follicle miniaturization.Taurine treatment,particularly at the high dose,effectively counteracted these effects:it promoted the telogen-to-anagen transition and improved hair growth scores.Histomorphometric analysis showed that taurine significantly restored DHT-induced reductions in dermal thickness,hair follicle count,hair bulb depth,and follicle size.Taurine treatment also reduced apoptosis and promoted the proliferation of hair follicle cells,as demonstrated by Ki67 and TUNEL assays.Crucially,RT-qPCR and Western blot analyses revealed that DHT significantly up-regulated the expression of AR,TGF‑β1,TGF‑β2,and DKK1 at both mRNA and protein levels in dorsal skin.Taurine administration markedly down-regulated the expression of these pathogenic factors,bringing them closer to the levels observed in the control group.Conclusion Taurine demonstrates significant efficacy in alleviating DHT-induced AGA in male C57BL/6 mice.Its protective effects are mediated through multi-faceted mechanisms.(1)Promoting hair follicle cycle progression:it accelerates the transition from telogen to anagen,counteracting DHT-induced prolongation of the telogen phase.(2)Modulating cellular dynamics:it stimulates the proliferation of hair matrix keratinocytes and reduces DHT-induced apoptosis within hair follicle cells.(3)Suppressing androgen-driven pathogenic pathways:it downregulates the expression of critical molecules in the AGA pathway,including AR,the cytokines TGF-β1 and TGF-β2,and the Wnt pathway inhibitor DKK1.Given its favorable safety profile and multi-targeted action,taurine emerges as a promising novel therapeutic candidate or adjunct for treating AGA.Further investigation into its clinical potential and precise molecular mechanisms is warranted.This study provides a robust preclinical foundation for considering taurine supplementation or topical application in hair loss management strategies.展开更多
为解决光伏发电功率预测中存在的高波动性问题,文章提出基于门控循环单元-贝叶斯最小二乘法(gated recurrent unit-bayesian least squares,GRU-BLS)的超短期光伏发电功率预测方法,构建GRU-BLS数学模型,GRU提取光伏发电功率时间序列特征...为解决光伏发电功率预测中存在的高波动性问题,文章提出基于门控循环单元-贝叶斯最小二乘法(gated recurrent unit-bayesian least squares,GRU-BLS)的超短期光伏发电功率预测方法,构建GRU-BLS数学模型,GRU提取光伏发电功率时间序列特征,BLS进行回归预测。同时,分析其预测流程,以提高超短期光伏发电功率预测准确性。结合案例分析可知,相比传统预测方法,GRU-BLS能显著提高预测精度。展开更多
Objective:Arrhythmia-induced cardiomyopathy(AIC)is a reversible dilated cardiomyopathy induced by rapid or irregular heartbeat.Acupuncture has a long history of use in the treatment of cardiac diseases,and Xinshu(BL15...Objective:Arrhythmia-induced cardiomyopathy(AIC)is a reversible dilated cardiomyopathy induced by rapid or irregular heartbeat.Acupuncture has a long history of use in the treatment of cardiac diseases,and Xinshu(BL15)is a key acupoint.However,the underlying mechanism of acupuncture at BL15 in the treatment of AIC has not yet been elucidated.Methods:AIC was induced in adult male Sprague-Dawley(SD)rats by continuous administration of acetylcholine(ACh)-CaCl2 and treatment with electroacupuncture(EA)at bilateral BL15.Echocardiography was used to evaluate cardiac function;the rotarod test for motor coordination and performance;hematoxylin and eosin(HE)staining for the morphology of ventricles;electrocardiogram for susceptibility,inducibility,and duration of atrial fibrillation(AF);and electrical and optical mapping in isolated rat hearts maintained by the Langendorff perfusion system for electrical conduction and intracellular handling,respectively.Reverse transcription quantitative polymerase chain reaction(RT-qPCR)and Western blotting were used to determine the levels of cardiac conduction and intracellular calcium-handling proteins in the ventricle.Results:The results showed that EA improved the ejection factor and morphological indices on echocardiography,restored motor coordination and performance,and alleviated ventricular dilation and AF onset.EA alleviates atrial conduction disorders,shortens APD80,and decreases calcium handling in rats with AIC.Cx43 was downregulated and CaMKII was upregulated,and both effects were reversed by EA treatment.Conclusion:Our study provides a novel AIC model with abnormal electrical propagation and calcium handling that can be protected by EA at BL15.This potential mechanism may be associated with the modulation of Cx43 and CaMKII expression.展开更多
Background:There is a gap in understanding the effects of different acupoints and treatment methods(acupuncture and moxibustion)on microcirculatory changes in the lumbar region.Objective:This study aimed to assess the...Background:There is a gap in understanding the effects of different acupoints and treatment methods(acupuncture and moxibustion)on microcirculatory changes in the lumbar region.Objective:This study aimed to assess the thermal effects of acupuncture at Weizhong(BL40),with acupuncture at Chize(LU5)and moxibustion at both acupoints as control interventions.Design,setting,participants and interventions:In this randomized controlled trial,140 healthy participants were equally divided into four groups:acupuncture at BL40(Acu-BL40),acupuncture at LU5(Acu-LU5),moxibustion at BL40(Mox-BL40)and moxibustion at LU5(Mox-LU5).Participants underwent a 30-minute session of their assigned treatment.Infrared thermal imaging was used to collect temperature data on the areas of interest for analysis.Main outcome measures:The primary measure was the change in average temperature of the observed area after the intervention.The secondary measures included periodic temperature changes every5 min and the temperature changes of the Governor Vessel and Bladder Meridian in the observed area after the intervention.Results:Significant interactions were observed between treatments and acupoints affecting temperature(P<0.001).The Acu-BL40 group showed a notably higher increase in mean temperature after 30 min compared to the Acu-LU5 and Mox-BL40 groups,with increases of 0.29(95%confidence interval[CI]=0.17 to 0.41)and 0.24(95%CI=0.08 to 0.41)℃,respectively.Conclusion:Acupuncture at BL40 acupoint can significantly increase the mean temperature in the observed area,highlighting the specific thermal effect of acupuncture compared to moxibustion in the lumbar area.This suggests a potential therapeutic benefit of acupuncture at BL40 for managing lumbar conditions.展开更多
We present an analysis of the cross-correlation between optical brightness and polarization degree in different types of blazars.The aim is to identify objects with simultaneous and consistent changes in characteristi...We present an analysis of the cross-correlation between optical brightness and polarization degree in different types of blazars.The aim is to identify objects with simultaneous and consistent changes in characteristics and to determine if this behavior relates to the types of objects studied.The analysis includes 23 objects:11 Flat Spectrum Radio Quasars(FSRQs),11 BL Lacs,and 1 radio galaxy.Dense overlapping observation series in the R band were used,collected over more than 10 yr as part of a monitoring program for bright blazars at St.Petersburg State University.The cross-correlation analysis procedure is detailed,including a method for assessing significance based on Monte Carlo simulations of synthetic light curves modeled using a Damped Random Walk.Significant correlations were found for 5 FSRQs and 1 BL Lac.No significant correlation was detected for 10 BL Lacs and 6 FSRQs.One object did not yield a reliable estimate.Based on the current results,we cannot claim that the observed difference in the behavior of these emission characteristics for different classes of blazars is significant.It is possible that observed correlations may be explained by the contribution of simultaneous flare events to the changes in flux and polarization degree curves,which occur more frequently in FSRQs.展开更多
Experimental mice play a critical role in biomedical research.The phenotype and application of different substrains vary due to genetic differentiation and variation.To ensure validity and reliability of results,it is...Experimental mice play a critical role in biomedical research.The phenotype and application of different substrains vary due to genetic differentiation and variation.To ensure validity and reliability of results,it is imperative to adhere to standardized experiments and controls.This paper objectively reviews the origin,differentiation,and phenotypic and genetic differences between the C57BL/6 and BALB/c mouse substrains.Furthermore,an optimal selection strategy is proposed based on the genetic quality control technology to facilitate the precise application of these two mouse substrains.展开更多
基金supported in part by the National Key Research and Development Project under Grant 2020YFB1806805partially funded through a grant from Qualcomm。
文摘6G is desired to support more intelligence networks and this trend attaches importance to the self-healing capability if degradation emerges in the cellular networks.As a primary component of selfhealing networks,fault detection is investigated in this paper.Considering the fast response and low timeand-computational consumption,it is the first time that the Online Broad Learning System(OBLS)is applied to identify outages in cellular networks.In addition,the Automatic-constructed Online Broad Learning System(AOBLS)is put forward to rationalize its structure and consequently avoid over-fitting and under-fitting.Furthermore,a multi-layer classification structure is proposed to further improve the classification performance.To face the challenges caused by imbalanced data in fault detection problems,a novel weighting strategy is derived to achieve the Multilayer Automatic-constructed Weighted Online Broad Learning System(MAWOBLS)and ensemble learning with retrained Support Vector Machine(SVM),denoted as EMAWOBLS,for superior treatment with this imbalance issue.Simulation results show that the proposed algorithm has excellent performance in detecting faults with satisfactory time usage.
基金supported by grants from The National Natural Science Foundation of China(31772690)the Natural Science Foundation of Shanxi Province(201701D121106)PhD Research Startup Foundation of Changzhi Medical College(BS202308)。
文摘Objective This study aimed to comprehensively investigate the potential protective effects and underlying mechanisms of taurine against dihydrotestosterone(DHT)-induced androgenetic alopecia(AGA)in male C57BL/6 mice,with a focus on hair follicle cycle modulation,cellular proliferation/apoptosis,and key related signaling pathways.Methods Six-week-old female C57BL/6 mice were initially used to assess the hair growth-promoting potential of taurine.After acclimatization,they were randomly assigned to three groups(n=8):control(regular drinking water),taurine(drinking water containing 1%taurine),and minoxidil(topical 2%minoxidil,positive control).For the AGA study,male C57BL/6 mice were randomly divided into five groups(n=8):control(physiological saline),DHT(model group,1 mg/d DHT),DHT+low-dose taurine(1 mg/d DHT+2 mg/d taurine),DHT+high-dose taurine(1 mg/d DHT+10 mg/d taurine),and DHT+minoxidil(positive control,1 mg/d DHT+topical 2%minoxidil).One day before treatment initiation,dorsal hair was shaved with scissors,and residual hair was removed using a depilatory cream.DHT and taurine were administered via daily intraperitoneal injection.Hair regrowth was assessed by photographing the depilated area at regular intervals and quantified using a four-point grading system(0-3).Dorsal skin samples were collected on day 14 for histological analysis(H&E staining),immunofluorescence staining(Ki67 for proliferation,TUNEL for apoptosis),ELISA(DHT quantification),RT-qPCR,and Western blot analysis to evaluate the expression of key genes and proteins(androgen receptor(AR),transforming growth factor(TGF)‑β1,TGF‑β2,Dickkopf-1(DKK1)).Results In female mice,taurine supplementation significantly accelerated hair growth,with effects comparable to minoxidil.This was evidenced by an earlier transition from pink(telogen)to black(anagen)skin and increased hair growth scores.Histological analysis showed that taurine increased hair follicle count and dermal thickness.Immunofluorescence confirmed enhanced keratinocyte proliferation in the hair matrix.In the DHTinduced AGA model,DHT significantly extended the telogen phase,inhibited hair growth,increased skin DHT content,and induced hair follicle miniaturization.Taurine treatment,particularly at the high dose,effectively counteracted these effects:it promoted the telogen-to-anagen transition and improved hair growth scores.Histomorphometric analysis showed that taurine significantly restored DHT-induced reductions in dermal thickness,hair follicle count,hair bulb depth,and follicle size.Taurine treatment also reduced apoptosis and promoted the proliferation of hair follicle cells,as demonstrated by Ki67 and TUNEL assays.Crucially,RT-qPCR and Western blot analyses revealed that DHT significantly up-regulated the expression of AR,TGF‑β1,TGF‑β2,and DKK1 at both mRNA and protein levels in dorsal skin.Taurine administration markedly down-regulated the expression of these pathogenic factors,bringing them closer to the levels observed in the control group.Conclusion Taurine demonstrates significant efficacy in alleviating DHT-induced AGA in male C57BL/6 mice.Its protective effects are mediated through multi-faceted mechanisms.(1)Promoting hair follicle cycle progression:it accelerates the transition from telogen to anagen,counteracting DHT-induced prolongation of the telogen phase.(2)Modulating cellular dynamics:it stimulates the proliferation of hair matrix keratinocytes and reduces DHT-induced apoptosis within hair follicle cells.(3)Suppressing androgen-driven pathogenic pathways:it downregulates the expression of critical molecules in the AGA pathway,including AR,the cytokines TGF-β1 and TGF-β2,and the Wnt pathway inhibitor DKK1.Given its favorable safety profile and multi-targeted action,taurine emerges as a promising novel therapeutic candidate or adjunct for treating AGA.Further investigation into its clinical potential and precise molecular mechanisms is warranted.This study provides a robust preclinical foundation for considering taurine supplementation or topical application in hair loss management strategies.
文摘为解决光伏发电功率预测中存在的高波动性问题,文章提出基于门控循环单元-贝叶斯最小二乘法(gated recurrent unit-bayesian least squares,GRU-BLS)的超短期光伏发电功率预测方法,构建GRU-BLS数学模型,GRU提取光伏发电功率时间序列特征,BLS进行回归预测。同时,分析其预测流程,以提高超短期光伏发电功率预测准确性。结合案例分析可知,相比传统预测方法,GRU-BLS能显著提高预测精度。
基金supported by the National Key R&D Program of China(2022YFC3500405,2019YFC1712105)The National Science Foundation of China(82374075)+1 种基金The National Comprehensive Traditional Chinese Medicine Reform Demonstration Zone Science and Technology Collaborative Development Project(GZY-KJS-SD-2024-046)Taishan Scholar Youth Project of Shandong Province(tsqn202306188).
文摘Objective:Arrhythmia-induced cardiomyopathy(AIC)is a reversible dilated cardiomyopathy induced by rapid or irregular heartbeat.Acupuncture has a long history of use in the treatment of cardiac diseases,and Xinshu(BL15)is a key acupoint.However,the underlying mechanism of acupuncture at BL15 in the treatment of AIC has not yet been elucidated.Methods:AIC was induced in adult male Sprague-Dawley(SD)rats by continuous administration of acetylcholine(ACh)-CaCl2 and treatment with electroacupuncture(EA)at bilateral BL15.Echocardiography was used to evaluate cardiac function;the rotarod test for motor coordination and performance;hematoxylin and eosin(HE)staining for the morphology of ventricles;electrocardiogram for susceptibility,inducibility,and duration of atrial fibrillation(AF);and electrical and optical mapping in isolated rat hearts maintained by the Langendorff perfusion system for electrical conduction and intracellular handling,respectively.Reverse transcription quantitative polymerase chain reaction(RT-qPCR)and Western blotting were used to determine the levels of cardiac conduction and intracellular calcium-handling proteins in the ventricle.Results:The results showed that EA improved the ejection factor and morphological indices on echocardiography,restored motor coordination and performance,and alleviated ventricular dilation and AF onset.EA alleviates atrial conduction disorders,shortens APD80,and decreases calcium handling in rats with AIC.Cx43 was downregulated and CaMKII was upregulated,and both effects were reversed by EA treatment.Conclusion:Our study provides a novel AIC model with abnormal electrical propagation and calcium handling that can be protected by EA at BL15.This potential mechanism may be associated with the modulation of Cx43 and CaMKII expression.
基金funded by the National Key Research and Development Program of China(No.2018YFC1704600)Top-notch Innovative Talent Cultivation Program of Zhejiang Chinese Medicine University(No.741100G00726)。
文摘Background:There is a gap in understanding the effects of different acupoints and treatment methods(acupuncture and moxibustion)on microcirculatory changes in the lumbar region.Objective:This study aimed to assess the thermal effects of acupuncture at Weizhong(BL40),with acupuncture at Chize(LU5)and moxibustion at both acupoints as control interventions.Design,setting,participants and interventions:In this randomized controlled trial,140 healthy participants were equally divided into four groups:acupuncture at BL40(Acu-BL40),acupuncture at LU5(Acu-LU5),moxibustion at BL40(Mox-BL40)and moxibustion at LU5(Mox-LU5).Participants underwent a 30-minute session of their assigned treatment.Infrared thermal imaging was used to collect temperature data on the areas of interest for analysis.Main outcome measures:The primary measure was the change in average temperature of the observed area after the intervention.The secondary measures included periodic temperature changes every5 min and the temperature changes of the Governor Vessel and Bladder Meridian in the observed area after the intervention.Results:Significant interactions were observed between treatments and acupoints affecting temperature(P<0.001).The Acu-BL40 group showed a notably higher increase in mean temperature after 30 min compared to the Acu-LU5 and Mox-BL40 groups,with increases of 0.29(95%confidence interval[CI]=0.17 to 0.41)and 0.24(95%CI=0.08 to 0.41)℃,respectively.Conclusion:Acupuncture at BL40 acupoint can significantly increase the mean temperature in the observed area,highlighting the specific thermal effect of acupuncture compared to moxibustion in the lumbar area.This suggests a potential therapeutic benefit of acupuncture at BL40 for managing lumbar conditions.
基金the National Science Foundation grant AST-2108622several NASA Fermi vip Investigator grants,the latest is 80NSSC23K1507supported by Fermi vip Investigator grants NNX08AW56G,NNX09AU10G,NNX12AO93G,and NNX15AU81G.
文摘We present an analysis of the cross-correlation between optical brightness and polarization degree in different types of blazars.The aim is to identify objects with simultaneous and consistent changes in characteristics and to determine if this behavior relates to the types of objects studied.The analysis includes 23 objects:11 Flat Spectrum Radio Quasars(FSRQs),11 BL Lacs,and 1 radio galaxy.Dense overlapping observation series in the R band were used,collected over more than 10 yr as part of a monitoring program for bright blazars at St.Petersburg State University.The cross-correlation analysis procedure is detailed,including a method for assessing significance based on Monte Carlo simulations of synthetic light curves modeled using a Damped Random Walk.Significant correlations were found for 5 FSRQs and 1 BL Lac.No significant correlation was detected for 10 BL Lacs and 6 FSRQs.One object did not yield a reliable estimate.Based on the current results,we cannot claim that the observed difference in the behavior of these emission characteristics for different classes of blazars is significant.It is possible that observed correlations may be explained by the contribution of simultaneous flare events to the changes in flux and polarization degree curves,which occur more frequently in FSRQs.
基金National Key R&D Program of China,Grant/Award Number:2021YFF0703200Key Technology Fund of the National Institutes for Food and Drug Control,Grant/Award Number:GJJS-2022-1-5。
文摘Experimental mice play a critical role in biomedical research.The phenotype and application of different substrains vary due to genetic differentiation and variation.To ensure validity and reliability of results,it is imperative to adhere to standardized experiments and controls.This paper objectively reviews the origin,differentiation,and phenotypic and genetic differences between the C57BL/6 and BALB/c mouse substrains.Furthermore,an optimal selection strategy is proposed based on the genetic quality control technology to facilitate the precise application of these two mouse substrains.
